Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,968,566 A→G L287L (TTA→TTG wecB → UDP‑N‑acetyl glucosamine ‑2‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,968,5660AG100.0% 885.0 / NA 271L287L (TTA→TTGwecBUDP‑N‑acetyl glucosamine ‑2‑epimerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (158/113);  total (158/113)

Only 100 of 272 total aligned reads displayed.

GGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTACCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCTGT  >  NC_000913/3968476‑3968655
                                                                                          |                                                                                         
 gTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCg                                                                                        <  2:4427356/93‑1 (MQ=255)
  tGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGt                                                                                       <  1:1546279/93‑1 (MQ=255)
   gCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGtt                                                                                      <  2:48428/93‑1 (MQ=255)
    cATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGttt                                                                                     >  2:1503053/1‑93 (MQ=255)
     aTCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTg                                                                                    >  1:4054211/1‑93 (MQ=255)
     aTCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTg                                                                                    >  2:5924967/1‑93 (MQ=255)
     aTCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTg                                                                                    >  1:2478625/1‑93 (MQ=255)
      tctcAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGt                                                                                   <  1:2561758/93‑1 (MQ=255)
      tctcAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGt                                                                                   <  1:4609848/93‑1 (MQ=255)
       ctcAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTc                                                                                  <  2:3041061/93‑1 (MQ=255)
        tcACCCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCt                                                                                 <  2:1100063/93‑1 (MQ=255)
          aaCCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTgg                                                                               >  1:1449804/1‑93 (MQ=255)
          aaCCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTgg                                                                               <  1:63509/93‑1 (MQ=255)
           aCCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGc                                                                              >  2:4129913/1‑93 (MQ=255)
           aCCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGc                                                                              >  2:112188/1‑93 (MQ=255)
             ccGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCtg                                                                            >  1:6103125/1‑93 (MQ=255)
               gAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCtgat                                                                          >  2:766871/1‑93 (MQ=255)
                 aCGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCtgatga                                                                        >  2:2022154/1‑93 (MQ=255)
                 aCGACAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCtgatga                                                                        <  1:3185489/93‑1 (MQ=255)
                   gTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAAc                                                                      <  2:3535239/93‑1 (MQ=255)
                   gTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAAc                                                                      >  1:838757/1‑93 (MQ=255)
                   gTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAAc                                                                      <  2:2078077/93‑1 (MQ=255)
                   gTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAAc                                                                      >  2:5294745/1‑93 (MQ=255)
                    tCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAAcc                                                                     <  2:5531143/93‑1 (MQ=255)
                    tCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAAcc                                                                     <  1:1054641/93‑1 (MQ=255)
                    tCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAAcc                                                                     >  2:3101871/1‑93 (MQ=255)
                     cAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCa                                                                    <  2:3343586/93‑1 (MQ=255)
                      agagAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCAc                                                                   >  1:4242616/1‑93 (MQ=255)
                      agagAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCAc                                                                   <  1:564388/93‑1 (MQ=255)
                       gagaACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACg                                                                  >  2:2811014/1‑93 (MQ=255)
                         gaACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGcc                                                                <  1:4528281/93‑1 (MQ=255)
                         gaACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGcc                                                                <  1:3028409/93‑1 (MQ=255)
                          aaCCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCt                                                               >  2:219485/1‑93 (MQ=255)
                           aCCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTg                                                              <  2:671133/93‑1 (MQ=255)
                             cGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGc                                                            >  2:3492100/1‑93 (MQ=255)
                                tCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGa                                                         <  1:3092399/93‑1 (MQ=255)
                                 cAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGAt                                                        <  1:283706/93‑1 (MQ=255)
                                 cAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGAt                                                        <  2:457189/93‑1 (MQ=255)
                                  aaTCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGAtt                                                       >  1:2120226/1‑93 (MQ=255)
                                   aTCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGAttt                                                      <  1:5496201/93‑1 (MQ=255)
                                    tCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGAtttt                                                     <  2:5064452/93‑1 (MQ=255)
                                    tCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGAtttt                                                     <  2:1215113/93‑1 (MQ=255)
                                        aTTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGAcc                                                 >  1:3742176/1‑93 (MQ=255)
                                         ttCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCg                                                >  2:4374427/1‑93 (MQ=255)
                                         ttCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCg                                                >  1:4797362/1‑93 (MQ=255)
                                          tCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGa                                               >  2:2300803/1‑93 (MQ=255)
                                             ggggCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTc                                            >  2:5026532/1‑93 (MQ=255)
                                              gggCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCa                                           <  2:1967860/93‑1 (MQ=255)
                                                  aTGTGAAAAATGTCATTCTGATCGATCCCCAGGGGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTTGCTGATTTTGACCGACTCAggcg                                       >  2:2467988/1‑93 (MQ=255)
                                                  aTGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAggcg                                       >  2:101059/1‑93 (MQ=255)
                                                  aTGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAggcg                                       >  1:1802566/1‑93 (MQ=255)
                                                     tgAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACTCCTGGCTGATTTTGACCGACTCAGGCGGCa                                    >  1:381781/1‑93 (MQ=255)
                                                     tgAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCa                                    >  1:5226926/1‑93 (MQ=255)
                                                      gAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCAt                                   >  1:2343878/1‑93 (MQ=255)
                                                        aaaaTGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTc                                 >  1:3341017/1‑93 (MQ=255)
                                                         aaaTGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCa                                <  2:2843627/93‑1 (MQ=255)
                                                          aaTGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAg                               >  2:4083103/1‑93 (MQ=255)
                                                          aaTGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAg                               >  1:274252/1‑93 (MQ=255)
                                                             gTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACAGACTCAGGCGGCCTTCAGgaa                            >  2:608966/1‑93 (MQ=255)
                                                              tCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGgaag                           <  2:901026/93‑1 (MQ=255)
                                                               cATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGgaaga                          <  1:2663089/93‑1 (MQ=255)
                                                                aTTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGgaagaa                         >  1:1259218/1‑93 (MQ=255)
                                                                aTTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGgaagaa                         <  1:4126665/93‑1 (MQ=255)
                                                                 ttCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGaagaag                        <  2:4015808/93‑1 (MQ=255)
                                                                   ttGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAgcg                      <  2:3256832/92‑1 (MQ=255)
                                                                   cTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAgcg                      >  1:345938/1‑93 (MQ=255)
                                                                    tGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAgcgc                     >  2:1897340/1‑93 (MQ=255)
                                                                    tGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAgcgc                     >  2:3695363/1‑93 (MQ=255)
                                                                    tGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAgcgc                     >  2:1553908/1‑93 (MQ=255)
                                                                    tGATCGATCCCCAGGAGTATTTGCCGGTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAgcgc                     >  1:4457616/1‑93 (MQ=255)
                                                                     gatcgatcCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGcc                    >  1:2927537/1‑93 (MQ=255)
                                                                      atcgatcCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCt                   <  1:467324/93‑1 (MQ=255)
                                                                      atcgatcCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCt                   >  1:1830757/1‑93 (MQ=255)
                                                                       tcgatcCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCtt                  <  2:1871236/93‑1 (MQ=255)
                                                                       tcgatcCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCtt                  <  2:5487264/93‑1 (MQ=255)
                                                                       tcgatcCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCAtt                  >  2:5635774/1‑93 (MQ=255)
                                                                        cgatcCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTc                 <  2:4912801/93‑1 (MQ=255)
                                                                         gatcCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCg                >  2:2952776/1‑93 (MQ=255)
                                                                          atcCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGc               >  2:641528/1‑93 (MQ=255)
                                                                          atcCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGc               >  2:58980/1‑93 (MQ=255)
                                                                           tcCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCt              <  1:1580095/93‑1 (MQ=255)
                                                                           tcCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCt              >  1:5997562/1‑93 (MQ=255)
                                                                            ccccAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTg             >  1:611957/1‑93 (MQ=255)
                                                                             cccAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTgg            >  2:4714304/1‑93 (MQ=255)
                                                                             cccAGGAGTATTTGCCGTTTATCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTgg            <  2:2727669/93‑1 (MQ=255)
                                                                              ccAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTggg           <  1:6255628/93‑1 (MQ=255)
                                                                              ccAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTggg           >  1:740917/1‑93 (MQ=255)
                                                                               cAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTgggg          >  1:2196700/1‑93 (MQ=255)
                                                                                aGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGa         <  2:6199322/93‑1 (MQ=255)
                                                                                aGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGa         >  1:1016434/1‑93 (MQ=255)
                                                                                 ggAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGaa        >  1:1614670/1‑93 (MQ=255)
                                                                                 ggAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGaa        >  2:386814/1‑93 (MQ=255)
                                                                                    gTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAAAcc     <  1:6040554/93‑1 (MQ=255)
                                                                                    gTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAAAcc     >  1:753672/1‑93 (MQ=255)
                                                                                     tATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGTGAAACCt    >  1:5888697/1‑93 (MQ=255)
                                                                                     tATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCt    >  1:5618892/1‑93 (MQ=255)
                                                                                     tATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCt    >  2:5438567/1‑93 (MQ=255)
                                                                                     tATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCt    >  1:3944498/1‑93 (MQ=255)
                                                                                      aTTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCtg   <  1:5285858/93‑1 (MQ=255)
                                                                                       tttGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGGATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCtgt  >  2:5349584/1‑93 (MQ=255)
                                                                                          |                                                                                         
GGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTACCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCTGT  >  NC_000913/3968476‑3968655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTACCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCTGTGCT  >  NC_000913/3968473‑3968658
                                                                                             |                                                                                            
TCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTG                                                                                              <  SRR4302533.1054753/94‑1 (MQ=60)
TCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTG                                                                                              <  SRR4302533.2406674/94‑1 (MQ=60)
  CGGTGCATCTCAGCCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCC                                                                                            <  SRR4302533.38840/94‑1 (MQ=60)
  CGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCC                                                                                            <  SRR4302533.2498865/94‑1 (MQ=60)
    GTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGT                                                                                          >  SRR4302533.2858467/1‑94 (MQ=60)
    GTGCATCTCAACCCGAACGCCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGT                                                                                          >  SRR4302533.2940995/1‑94 (MQ=60)
     TGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTT                                                                                         <  SRR4302533.1555598/94‑1 (MQ=60)
     TGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTT                                                                                         >  SRR4302533.4525548/1‑94 (MQ=60)
     GGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTT                                                                                         <  SRR4302533.3950861/94‑1 (MQ=60)
      GCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTT                                                                                        >  SRR4302533.1579712/1‑94 (MQ=60)
       CATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTG                                                                                       >  SRR4302533.1214179/1‑94 (MQ=60)
       CATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTG                                                                                       <  SRR4302533.319739/94‑1 (MQ=60)
       CATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTG                                                                                       >  SRR4302533.4078897/1‑94 (MQ=60)
       CATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTG                                                                                       >  SRR4302533.2493911/1‑94 (MQ=60)
         TCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTC                                                                                     <  SRR4302533.4637765/94‑1 (MQ=60)
         TCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTC                                                                                     <  SRR4302533.2577531/94‑1 (MQ=60)
            CAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGG                                                                                  <  SRR4302533.2545308/94‑1 (MQ=60)
            CAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGG                                                                                  >  SRR4302533.6063501/1‑94 (MQ=60)
            CAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGG                                                                                  >  SRR4302533.1458552/1‑94 (MQ=60)
             AACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGC                                                                                 >  SRR4302533.424909/1‑94 (MQ=60)
             AACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGC                                                                                 <  SRR4302533.3077815/94‑1 (MQ=60)
             AACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGC                                                                                 <  SRR4302533.63924/94‑1 (MQ=60)
              ACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCT                                                                                <  SRR4302533.867812/94‑1 (MQ=60)
               CCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTG                                                                               >  SRR4302533.6139967/1‑94 (MQ=60)
                   AACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGCATTTGCCGTTTGTCTGGCTGATGA                                                                           >  SRR4302533.2087784/1‑94 (MQ=60)
                    ACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAA                                                                          <  SRR4302533.2241548/94‑1 (MQ=60)
                    ACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAA                                                                          <  SRR4302533.1610615/94‑1 (MQ=60)
                    ACGACAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAA                                                                          <  SRR4302533.3205081/94‑1 (MQ=60)
                     CGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAAC                                                                         >  SRR4302533.1828891/1‑94 (MQ=60)
                     CGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAAC                                                                         >  SRR4302533.843670/1‑94 (MQ=60)
                       TCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCA                                                                       <  SRR4302533.1060848/94‑1 (MQ=60)
                        CAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCAC                                                                      >  SRR4302533.4268232/1‑94 (MQ=60)
                        CAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCAC                                                                      <  SRR4302533.2445599/94‑1 (MQ=60)
                        CAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCAC                                                                      >  SRR4302533.5522362/1‑94 (MQ=60)
                        CAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCAC                                                                      >  SRR4302533.5846323/1‑94 (MQ=60)
                        CAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCAC                                                                      <  SRR4302533.1045304/94‑1 (MQ=60)
                        CAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATCTGCCGTTTGTCTGGCTGATGAACCAC                                                                      >  SRR4302533.906292/1‑94 (MQ=60)
                         AGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACG                                                                     <  SRR4302533.567731/94‑1 (MQ=60)
                         AGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACG                                                                     >  SRR4302533.281867/1‑94 (MQ=60)
                          GAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGC                                                                    >  SRR4302533.704069/1‑94 (MQ=60)
                            GAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCT                                                                  <  SRR4302533.4555705/94‑1 (MQ=60)
                            GAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCT                                                                  <  SRR4302533.3046942/94‑1 (MQ=60)
                             AACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTG                                                                 <  SRR4302533.1752220/94‑1 (MQ=60)
                              ACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGG                                                                <  SRR4302533.1660057/94‑1 (MQ=60)
                              ACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGG                                                                <  SRR4302533.6294697/94‑1 (MQ=60)
                               CCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGC                                                               <  SRR4302533.1128904/94‑1 (MQ=60)
                                 GGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTG                                                             >  SRR4302533.6133097/1‑94 (MQ=60)
                                   TCGATCGCATTCTGGGGCATGTGAACAATGTCATTCTGATCGATCCCCAGGAGTATTTGCAGTTTGTCTGGCTGATGAACCACGCCTGGCTGAT                                                           <  SRR4302533.6333486/94‑1 (MQ=60)
                                   TCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGAT                                                           <  SRR4302533.3111413/94‑1 (MQ=60)
                                    CAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATT                                                          >  SRR4302533.2133301/1‑94 (MQ=60)
                                    CAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATT                                                          <  SRR4302533.285442/94‑1 (MQ=60)
                                      ATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTT                                                        <  SRR4302533.4661325/94‑1 (MQ=60)
                                      ATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTT                                                        >  SRR4302533.5260468/1‑94 (MQ=60)
                                      ATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTT                                                        <  SRR4302533.5529352/94‑1 (MQ=60)
                                       TCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTG                                                       >  SRR4302533.5263501/1‑94 (MQ=60)
                                         GCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGAC                                                     <  SRR4302533.329749/94‑1 (MQ=60)
                                          CATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACC                                                    <  SRR4302533.828032/94‑1 (MQ=60)
                                          CATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACC                                                    >  SRR4302533.3765126/1‑94 (MQ=60)
                                          CATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACC                                                    <  SRR4302533.359627/94‑1 (MQ=60)
                                           ATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCG                                                   >  SRR4302533.4826422/1‑94 (MQ=60)
                                             TCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGAC                                                 <  SRR4302533.2850472/94‑1 (MQ=60)
                                              CTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACT                                                <  SRR4302533.4520293/94‑1 (MQ=60)
                                              CTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACT                                                >  SRR4302533.5243961/1‑94 (MQ=60)
                                               TGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGAATC                                               >  SRR4302533.5587382/1‑94 (MQ=60)
                                                GGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCA                                              >  SRR4302533.6193149/1‑94 (MQ=60)
                                                  GGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGG                                            <  SRR4302533.3187879/94‑1 (MQ=60)
                                                   GCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGC                                           <  SRR4302533.1613472/94‑1 (MQ=60)
                                                   GCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGC                                           <  SRR4302533.5609871/94‑1 (MQ=60)
                                                    CATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCG                                          <  SRR4302533.6023644/94‑1 (MQ=60)
                                                    CATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCG                                          >  SRR4302533.3785176/1‑94 (MQ=60)
                                                    CATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCG                                          >  SRR4302533.210552/1‑94 (MQ=60)
                                                    CATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCG                                          >  SRR4302533.1813538/1‑94 (MQ=60)
                                                    CATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCG                                          >  SRR4302533.4071006/1‑94 (MQ=60)
                                                     ATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGG                                         >  SRR4302533.4636787/1‑94 (MQ=60)
                                                     ATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGG                                         >  SRR4302533.1356896/1‑94 (MQ=60)
                                                     ATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGG                                         >  SRR4302533.3881023/1‑94 (MQ=60)
                                                     ATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGG                                         >  SRR4302533.3778047/1‑94 (MQ=60)
                                                       GTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACTCCTGGCTGATTTTGACCGACTCAGGCGGCA                                       >  SRR4302533.384083/1‑94 (MQ=60)
                                                       GTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCA                                       >  SRR4302533.5258376/1‑94 (MQ=60)
                                                        TGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCAT                                      >  SRR4302533.2358387/1‑94 (MQ=60)
                                                        TGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCAT                                      >  SRR4302533.6254775/1‑94 (MQ=60)
                                                          AAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTC                                    >  SRR4302533.3361665/1‑94 (MQ=60)
                                                          AAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTC                                    >  SRR4302533.1582888/1‑94 (MQ=60)
                                                            AAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAG                                  >  SRR4302533.4803896/1‑94 (MQ=60)
                                                            AAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAG                                  <  SRR4302533.2408048/94‑1 (MQ=60)
                                                            AAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAG                                  >  SRR4302533.275921/1‑94 (MQ=60)
                                                            AAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAG                                  >  SRR4302533.3016531/1‑94 (MQ=60)
                                                              ATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGA                                >  SRR4302533.2653200/1‑94 (MQ=60)
                                                              ATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGA                                >  SRR4302533.5086216/1‑94 (MQ=60)
                                                              ATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGA                                >  SRR4302533.2146351/1‑94 (MQ=60)
                                                               TGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAA                               >  SRR4302533.5783288/1‑94 (MQ=60)
                                                                GTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAG                              <  SRR4302533.786813/94‑1 (MQ=60)
                                                                  CATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAA                            >  SRR4302533.1266749/1‑94 (MQ=60)
                                                                  CATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAA                            >  SRR4302533.73775/1‑94 (MQ=60)
                                                                  CATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAA                            <  SRR4302533.2679439/94‑1 (MQ=60)
                                                                   ATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAG                           <  SRR4302533.4151715/94‑1 (MQ=60)
                                                                    TTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGC                          <  SRR4302533.2154897/94‑1 (MQ=60)
                                                                     TCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCG                         >  SRR4302533.348050/1‑94 (MQ=60)
                                                                      CTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGC                        >  SRR4302533.2705255/1‑94 (MQ=60)
                                                                      CTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGC                        >  SRR4302533.6040546/1‑94 (MQ=60)
                                                                      CTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGC                        <  SRR4302533.1510359/94‑1 (MQ=60)
                                                                      CTGATCGATCCCCAGGAGTATTTGCCGGTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGC                        >  SRR4302533.4484571/1‑94 (MQ=60)
                                                                       TGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCC                       >  SRR4302533.2945450/1‑94 (MQ=60)
                                                                        GATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCT                      >  SRR4302533.756490/1‑94 (MQ=60)
                                                                        GATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCT                      >  SRR4302533.1841932/1‑94 (MQ=60)
                                                                         ATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTT                     <  SRR4302533.1366358/94‑1 (MQ=60)
                                                                         ATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTT                     <  SRR4302533.470114/94‑1 (MQ=60)
                                                                          TCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTC                    >  SRR4302533.4692133/1‑94 (MQ=60)
                                                                          TCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTC                    <  SRR4302533.2570685/94‑1 (MQ=60)
                                                                           CGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCG                   <  SRR4302533.4577961/94‑1 (MQ=60)
                                                                             ATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCT                 >  SRR4302533.2676646/1‑94 (MQ=60)
                                                                             ATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCT                 >  SRR4302533.6033887/1‑94 (MQ=60)
                                                                             ATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCACGCGGCATTCAGGAATAAGCGCCTTCGCT                 >  SRR4302533.5655540/1‑94 (MQ=60)
                                                                              TCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTG                <  SRR4302533.1589613/94‑1 (MQ=60)
                                                                              TCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTG                >  SRR4302533.321646/1‑94 (MQ=60)
                                                                              TCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTG                >  SRR4302533.615554/1‑94 (MQ=60)
                                                                                CCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGG              >  SRR4302533.745277/1‑94 (MQ=60)
                                                                                CCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGG              <  SRR4302533.4241724/94‑1 (MQ=60)
                                                                                CCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGG              <  SRR4302533.6152399/94‑1 (MQ=60)
                                                                                 CCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGGTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGG             >  SRR4302533.3394410/1‑94 (MQ=60)
                                                                                 CCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGG             >  SRR4302533.2210236/1‑94 (MQ=60)
                                                                                 CCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGG             <  SRR4302533.6293038/94‑1 (MQ=60)
                                                                                  CAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGA            >  SRR4302533.1022408/1‑94 (MQ=60)
                                                                                   AGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAA           >  SRR4302533.1624459/1‑94 (MQ=60)
                                                                                     GAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAAAC         <  SRR4302533.438242/94‑1 (MQ=60)
                                                                                      AGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAAACC        <  SRR4302533.362542/94‑1 (MQ=60)
                                                                                      AGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAAACC        >  SRR4302533.758110/1‑94 (MQ=60)
                                                                                      AGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAAACC        >  SRR4302533.5674333/1‑94 (MQ=60)
                                                                                       GTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGTGAAACCT       >  SRR4302533.5924274/1‑94 (MQ=60)
                                                                                       GTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCT       >  SRR4302533.4144852/1‑94 (MQ=60)
                                                                                       GTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCT       >  SRR4302533.3968560/1‑94 (MQ=60)
                                                                                       GTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCT       <  SRR4302533.6077158/94‑1 (MQ=60)
                                                                                       GTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCT       >  SRR4302533.5652853/1‑94 (MQ=60)
                                                                                         ATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCTGT     <  SRR4302533.6106533/94‑1 (MQ=60)
                                                                                         ATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCTGT     <  SRR4302533.5317521/94‑1 (MQ=60)
                                                                                          TTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGCCTCAGGCGGCATTCAGGAAGAAGCGCCTTaccgccgggccagtcat    <  SRR4302533.3579881/94‑18 (MQ=60)
                                                                                          TTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCTGTG    <  SRR4302533.4140292/94‑1 (MQ=60)
                                                                                          TTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCTGTG    <  SRR4302533.310759/94‑1 (MQ=60)
                                                                                            TGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCTGTGCT  >  SRR4302533.3891571/1‑94 (MQ=60)
                                                                                             |                                                                                            
TCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTACCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCTGTGCT  >  NC_000913/3968473‑3968658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: