Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,972,641 G→A R145H (CGC→CAC)  wecD → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,972,6410GA99.5% 775.4 / ‑5.4 214R145H (CGC→CAC) wecDhypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (87/126);  total (87/127)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.66e-01

Only 100 of 219 total aligned reads displayed.

AATGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATCGTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAG  >  NC_000913/3972553‑3972724
                                                                                        |                                                                                   
aaTGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGt                                                                                 >  2:3670512/1‑93 (MQ=255)
 aTGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTa                                                                                <  2:1876845/93‑1 (MQ=255)
  tGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTaa                                                                               >  1:2636237/1‑93 (MQ=255)
   gCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCATACGGCGCTAAACTGGGCGTATGCTCACGGTaaa                                                                              <  1:6100815/93‑1 (MQ=255)
   gCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTaaa                                                                              <  1:3892424/93‑1 (MQ=255)
     gACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAaac                                                                            >  1:5014049/1‑93 (MQ=255)
     gACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAaac                                                                            >  2:4193222/1‑93 (MQ=255)
     gACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAaac                                                                            >  1:3232726/1‑93 (MQ=255)
      aCAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAaaca                                                                           >  2:8535/1‑93 (MQ=255)
      aCAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAaaca                                                                           <  1:5741783/93‑1 (MQ=255)
         gATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACt                                                                        >  2:3077631/1‑93 (MQ=255)
         gATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACt                                                                        <  1:1804062/93‑1 (MQ=255)
           tGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACttt                                                                      <  1:4119143/93‑1 (MQ=255)
           tGCGCGAATTGGCATGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACttt                                                                      <  1:3601669/93‑1 (MQ=255)
            gcgcGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTg                                                                     <  2:3750722/93‑1 (MQ=255)
             cgcgAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGc                                                                    <  1:1607318/93‑1 (MQ=255)
              gcgAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCg                                                                   >  1:76236/1‑93 (MQ=255)
               cgAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCgg                                                                  <  1:5598442/93‑1 (MQ=255)
               cgAATTGACCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCgg                                                                  <  2:2075015/93‑1 (MQ=255)
                 aaTTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGt                                                                <  1:4199707/93‑1 (MQ=255)
                 aaTTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGt                                                                <  2:1543113/93‑1 (MQ=255)
                  aTTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTg                                                               <  2:745453/93‑1 (MQ=255)
                  aTTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTg                                                               >  2:5075796/1‑93 (MQ=255)
                  aTTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTg                                                               <  2:1118480/93‑1 (MQ=255)
                   ttGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTgg                                                              <  2:5317211/93‑1 (MQ=255)
                   ttGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTgg                                                              <  2:1361123/93‑1 (MQ=255)
                     ggCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCg                                                            <  1:2133259/93‑1 (MQ=255)
                      gCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGa                                                           <  2:897732/93‑1 (MQ=255)
                        ctgctgGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGAcc                                                         <  1:3304267/93‑1 (MQ=255)
                         tgctgGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGCAAAACAACTTTGCGGGTGGCGAccc                                                        >  2:5004806/1‑93 (MQ=255)
                         tgctgGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGAccc                                                        <  1:661780/93‑1 (MQ=255)
                         tgctgGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGAccc                                                        >  1:2340690/1‑93 (MQ=255)
                           ctggctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCaa                                                      <  1:4819841/93‑1 (MQ=255)
                            tggctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCaaa                                                     <  1:5673008/93‑1 (MQ=255)
                             ggctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAAt                                                    >  2:4396487/1‑93 (MQ=255)
                             ggctagACGCGGTGCAGGTGCTGAGCTGATGCAAACGTCGCTAAACTGTGCGTATGCTCACGGTAAAACACCTTTGCGGGTGGCGACCCAAAt                                                    <  1:1815227/93‑1 (MQ=255)
                              gctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATg                                                   <  1:2322984/93‑1 (MQ=255)
                              gctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATg                                                   <  2:1064995/93‑1 (MQ=255)
                              gctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATg                                                   <  1:5705840/93‑1 (MQ=255)
                              gctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATg                                                   >  1:2707608/1‑93 (MQ=255)
                               ctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATgg                                                  <  1:6243738/93‑1 (MQ=255)
                               ctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATgg                                                  <  1:4483015/93‑1 (MQ=255)
                                tggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATggg                                                 <  2:1427926/93‑1 (MQ=255)
                                tggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATggg                                                 >  1:3524514/1‑93 (MQ=255)
                                tggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATggg                                                 >  2:3708717/1‑93 (MQ=255)
                                 ggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGc                                                >  1:3487763/1‑93 (MQ=255)
                                 ggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGc                                                <  2:552637/93‑1 (MQ=255)
                                 ggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGc                                                <  2:3242446/93‑1 (MQ=255)
                                 ggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGc                                                <  2:5837926/93‑1 (MQ=255)
                                 ggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGc                                                >  1:4555949/1‑93 (MQ=255)
                                  gACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCa                                               >  2:2594470/1‑93 (MQ=255)
                                  gACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCa                                               >  2:4038728/1‑93 (MQ=255)
                                   aCGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCaa                                              <  1:6128453/93‑1 (MQ=255)
                                     gcgGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAaca                                            <  2:1689923/93‑1 (MQ=255)
                                      cgGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAacac                                           >  1:5334523/1‑93 (MQ=255)
                                      cgGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAacac                                           <  2:3833332/93‑1 (MQ=255)
                                         tGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAccgc                                        <  1:3086773/93‑1 (MQ=255)
                                           cAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAccgccg                                      >  2:3536467/1‑93 (MQ=255)
                                            aGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACcgccgc                                     <  2:3933688/93‑1 (MQ=255)
                                              gTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCgcgc                                   <  1:1263472/93‑1 (MQ=255)
                                              gTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCgcgc                                   <  2:2504680/93‑1 (MQ=255)
                                              gTGCTGAGCTGATGCAAACCGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCgcgc                                   >  2:6140264/1‑93 (MQ=255)
                                                   gagctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTaaa                              <  1:3009863/93‑1 (MQ=255)
                                                    agctgaTGCAGACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAAc                             <  2:3312131/93‑1 (MQ=255)
                                                    agctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAAc                             <  1:5829370/93‑1 (MQ=255)
                                                      ctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGa                           >  2:1191622/1‑93 (MQ=255)
                                                      ctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGa                           >  1:2792232/1‑93 (MQ=255)
                                                       tgaTGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGat                          >  2:5683521/1‑93 (MQ=255)
                                                       tgaTGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGat                          >  2:684299/1‑93 (MQ=255)
                                                        gaTGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGata                         >  2:4696453/1‑93 (MQ=255)
                                                        gaTGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGata                         >  1:1337130/1‑93 (MQ=255)
                                                        gaTGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGata                         >  2:1028328/1‑93 (MQ=255)
                                                        gaTGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGata                         >  1:5880573/1‑93 (MQ=255)
                                                         aTGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGatac                        <  1:1073264/93‑1 (MQ=255)
                                                         aTGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGAGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGatac                        <  1:3389431/93‑1 (MQ=255)
                                                          tGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGataca                       <  2:2799147/93‑1 (MQ=255)
                                                          tGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGataca                       >  2:4540461/1‑93 (MQ=255)
                                                          tGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGataca                       <  1:5856793/93‑1 (MQ=255)
                                                          tGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGataca                       <  1:5116631/93‑1 (MQ=255)
                                                            cAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGatacata                     >  1:1483664/1‑93 (MQ=255)
                                                              aaCGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGAtacataca                   <  2:5726226/93‑1 (MQ=255)
                                                              aaCGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGAtacataca                   >  1:1909740/1‑93 (MQ=255)
                                                               aCGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACaa                  <  1:1690247/93‑1 (MQ=255)
                                                                 ggCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAg                >  2:3476136/1‑93 (MQ=255)
                                                                    gcTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgtgg             >  1:3117887/1‑93 (MQ=255)
                                                                     cTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgtggt            <  2:5909557/93‑1 (MQ=255)
                                                                     cTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgtggt            <  1:5243546/93‑1 (MQ=255)
                                                                      tAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAGACGATACATACAAAgtggtg           >  1:4203993/1‑93 (MQ=255)
                                                                      tAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgtggtg           <  2:1196511/93‑1 (MQ=255)
                                                                       aaaCTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGc          >  2:2447085/1‑93 (MQ=255)
                                                                       aaaCTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGc          <  2:2284423/93‑1 (MQ=255)
                                                                        aaCTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCg         >  1:1470502/1‑93 (MQ=255)
                                                                         aCTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGa        <  2:6290791/93‑1 (MQ=255)
                                                                           tgtgCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAAt      <  1:465365/90‑1 (MQ=255)
                                                                           tGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAAt      <  2:4279176/93‑1 (MQ=255)
                                                                            gggCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATg     <  2:3160017/93‑1 (MQ=255)
                                                                              gCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTa   <  1:3109213/93‑1 (MQ=255)
                                                                              gCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTa   <  1:435271/93‑1 (MQ=255)
                                                                              gCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTa   <  1:111028/93‑1 (MQ=255)
                                                                               cGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAg  <  1:3718171/93‑1 (MQ=255)
                                                                                        |                                                                                   
AATGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATCGTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAG  >  NC_000913/3972553‑3972724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATCGTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAGAAAGCACCGC  >  NC_000913/3972554‑3972734
                                                                                       |                                                                                             
ATGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAA                                                                                         >  SRR4302533.2652440/1‑94 (MQ=60)
  GCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCATACGGCGCTAAACTGGGCGTATGCTCACGGTAAAA                                                                                       <  SRR4302533.6137654/94‑1 (MQ=60)
  GCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAA                                                                                       <  SRR4302533.5361680/94‑1 (MQ=60)
  GCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAA                                                                                       <  SRR4302533.3916182/94‑1 (MQ=60)
   CGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAAC                                                                                      >  SRR4302533.3252645/1‑94 (MQ=60)
   CGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAAC                                                                                      >  SRR4302533.5044427/1‑94 (MQ=60)
    GACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACA                                                                                     <  SRR4302533.2114693/94‑1 (MQ=60)
    GACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACA                                                                                     >  SRR4302533.1969960/1‑94 (MQ=60)
    GACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGCATGCTCACGGTAAAACA                                                                                     >  SRR4302533.182251/1‑94 (MQ=60)
     ACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAA                                                                                    <  SRR4302533.5776433/94‑1 (MQ=60)
     ACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAA                                                                                    <  SRR4302533.5786075/94‑1 (MQ=60)
        GATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTT                                                                                 <  SRR4302533.1815042/94‑1 (MQ=60)
          TGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTG                                                                               <  SRR4302533.4144159/94‑1 (MQ=60)
          TGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTG                                                                               <  SRR4302533.547826/94‑1 (MQ=60)
          TGCGCGAATTGGCATGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTG                                                                               <  SRR4302533.3623833/94‑1 (MQ=60)
           GCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGC                                                                              <  SRR4302533.6254886/94‑1 (MQ=60)
            CGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCG                                                                             >  SRR4302533.76728/1‑94 (MQ=60)
            CGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCG                                                                             <  SRR4302533.1617072/94‑1 (MQ=60)
              CGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGG                                                                           <  SRR4302533.1714098/94‑1 (MQ=60)
              CGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGG                                                                           <  SRR4302533.6155246/94‑1 (MQ=60)
              CGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGG                                                                           <  SRR4302533.3929666/94‑1 (MQ=60)
              CGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGG                                                                           <  SRR4302533.5632266/94‑1 (MQ=60)
               GAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAGCAACTTTGCGGGT                                                                          >  SRR4302533.3626542/1‑94 (MQ=60)
                AATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTG                                                                         <  SRR4302533.1591576/94‑1 (MQ=60)
                AATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTG                                                                         >  SRR4302533.4150482/1‑94 (MQ=60)
                AATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTG                                                                         <  SRR4302533.4225121/94‑1 (MQ=60)
                 ATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGG                                                                        <  SRR4302533.3133806/94‑1 (MQ=60)
                  TTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGC                                                                       >  SRR4302533.2537606/1‑94 (MQ=60)
                    GGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGA                                                                     <  SRR4302533.2146415/94‑1 (MQ=60)
                       CTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCC                                                                  <  SRR4302533.3324671/94‑1 (MQ=60)
                       CTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCC                                                                  <  SRR4302533.2757904/94‑1 (MQ=60)
                       CTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCC                                                                  >  SRR4302533.2355182/1‑94 (MQ=60)
                       CTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCC                                                                  >  SRR4302533.1636805/1‑94 (MQ=60)
                       CTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGGGGCGACCC                                                                  >  SRR4302533.2341762/1‑94 (MQ=60)
                        TGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCA                                                                 <  SRR4302533.665685/94‑1 (MQ=60)
                          CTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAA                                                               <  SRR4302533.4849048/94‑1 (MQ=60)
                           TGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAAT                                                              >  SRR4302533.279507/1‑94 (MQ=60)
                           TGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAAT                                                              >  SRR4302533.744975/1‑94 (MQ=60)
                           TGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAAT                                                              <  SRR4302533.5707269/94‑1 (MQ=60)
                            GGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATG                                                             >  SRR4302533.2724218/1‑94 (MQ=60)
                            GGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATG                                                             >  SRR4302533.149205/1‑94 (MQ=60)
                            GGCTAGACGCGGTGCAGGTGCTGAGCTGATGCAAACGTCGCTAAACTGTGCGTATGCTCACGGTAAAACACCTTTGCGGGTGGCGACCCAAATG                                                             <  SRR4302533.1826311/94‑1 (MQ=60)
                             GCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGG                                                            <  SRR4302533.2337361/94‑1 (MQ=60)
                             GCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGG                                                            <  SRR4302533.5740303/94‑1 (MQ=60)
                              CTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGG                                                           <  SRR4302533.4510163/94‑1 (MQ=60)
                              CTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGG                                                           <  SRR4302533.6281112/94‑1 (MQ=60)
                              CTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGG                                                           >  SRR4302533.3546235/1‑94 (MQ=60)
                              CTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGG                                                           <  SRR4302533.4780420/94‑1 (MQ=60)
                               TGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGC                                                          >  SRR4302533.4583581/1‑94 (MQ=60)
                               TGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGC                                                          >  SRR4302533.3509268/1‑94 (MQ=60)
                                GGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCA                                                         >  SRR4302533.3861166/1‑94 (MQ=60)
                                GGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCA                                                         <  SRR4302533.226971/94‑1 (MQ=60)
                                  ACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAAC                                                       <  SRR4302533.6165386/94‑1 (MQ=60)
                                   CGCGGTGCAGGTGCTGAGCTGATGCACACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACA                                                      <  SRR4302533.3115535/94‑1 (MQ=60)
                                   CGCGGTGCAGGTGCTGAGCTAATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACA                                                      <  SRR4302533.3266296/94‑1 (MQ=60)
                                    GCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAC                                                     <  SRR4302533.1579356/94‑1 (MQ=60)
                                    GCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAC                                                     <  SRR4302533.3650621/94‑1 (MQ=60)
                                    GCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAC                                                     >  SRR4302533.5366499/1‑94 (MQ=60)
                                     CGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACC                                                    <  SRR4302533.3275955/94‑1 (MQ=60)
                                       GTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGC                                                  <  SRR4302533.3390349/94‑1 (MQ=60)
                                       GTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGC                                                  >  SRR4302533.4566418/1‑94 (MQ=60)
                                        TGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCC                                                 <  SRR4302533.3527537/94‑1 (MQ=60)
                                        TGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCC                                                 <  SRR4302533.3105751/94‑1 (MQ=60)
                                           AGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCG                                              <  SRR4302533.4228744/94‑1 (MQ=60)
                                             GTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCT                                            <  SRR4302533.1271031/94‑1 (MQ=60)
                                                CTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAA                                         <  SRR4302533.4905474/94‑1 (MQ=60)
                                                 TGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAA                                        >  SRR4302533.5000282/1‑94 (MQ=60)
                                                  GAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAAC                                       <  SRR4302533.3028280/94‑1 (MQ=60)
                                                  GAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAAC                                       <  SRR4302533.299740/94‑1 (MQ=60)
                                                  GAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAAC                                       <  SRR4302533.424155/94‑1 (MQ=60)
                                                   AGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACG                                      <  SRR4302533.1092988/94‑1 (MQ=60)
                                                   AGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACG                                      <  SRR4302533.5864596/94‑1 (MQ=60)
                                                    GCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGA                                     >  SRR4302533.2809406/1‑94 (MQ=60)
                                                     CTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGAT                                    >  SRR4302533.5109252/1‑94 (MQ=60)
                                                      TGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATA                                   >  SRR4302533.1345155/1‑94 (MQ=60)
                                                      TGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATA                                   >  SRR4302533.5916109/1‑94 (MQ=60)
                                                      TGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATA                                   >  SRR4302533.1830178/1‑94 (MQ=60)
                                                        ATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACA                                 <  SRR4302533.1079591/94‑1 (MQ=60)
                                                        ATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACA                                 <  SRR4302533.576528/94‑1 (MQ=60)
                                                        ATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGAGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACA                                 <  SRR4302533.3410362/94‑1 (MQ=60)
                                                         TGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACAT                                <  SRR4302533.5892199/94‑1 (MQ=60)
                                                         TGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACAT                                <  SRR4302533.5147553/94‑1 (MQ=60)
                                                          GCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATA                               >  SRR4302533.1492619/1‑94 (MQ=60)
                                                          GCAAACGGCGCCAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATA                               <  SRR4302533.3605576/94‑1 (MQ=60)
                                                            AAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACA                             >  SRR4302533.1921391/1‑94 (MQ=60)
                                                             AACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAA                            >  SRR4302533.2694656/1‑94 (MQ=60)
                                                              ACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAA                           <  SRR4302533.1700486/94‑1 (MQ=60)
                                                                GGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGT                         <  SRR4302533.446863/94‑1 (MQ=60)
                                                                 GCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTG                        <  SRR4302533.834054/94‑1 (MQ=60)
                                                                 GCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTG                        <  SRR4302533.3247038/94‑1 (MQ=60)
                                                                  CGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGG                       <  SRR4302533.518011/94‑1 (MQ=60)
                                                                  CGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGG                       >  SRR4302533.3137059/1‑94 (MQ=60)
                                                                  CGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGG                       <  SRR4302533.5865953/94‑1 (MQ=60)
                                                                   GCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGT                      >  SRR4302533.1315572/1‑94 (MQ=60)
                                                                   GCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGT                      >  SRR4302533.1418265/1‑94 (MQ=60)
                                                                   GCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGT                      >  SRR4302533.4593608/1‑94 (MQ=60)
                                                                   GCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGT                      >  SRR4302533.1216717/1‑94 (MQ=60)
                                                                   GCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGT                      >  SRR4302533.5900121/1‑94 (MQ=60)
                                                                    CTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAGACGATACATACAAAGTGGTG                     >  SRR4302533.4229424/1‑94 (MQ=60)
                                                                    CTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTG                     >  SRR4302533.6309021/1‑94 (MQ=60)
                                                                    CTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTG                     <  SRR4302533.5275063/94‑1 (MQ=60)
                                                                    CTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTG                     <  SRR4302533.3034809/94‑1 (MQ=60)
                                                                      AAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCG                   >  SRR4302533.1479364/1‑94 (MQ=60)
                                                                       AACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGA                  >  SRR4302533.3439545/1‑94 (MQ=60)
                                                                         CTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAAT                >  SRR4302533.4265152/1‑94 (MQ=60)
                                                                           GGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGT              >  SRR4302533.6025355/1‑94 (MQ=60)
                                                                            GGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTA             <  SRR4302533.595458/94‑1 (MQ=60)
                                                                             GCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAG            <  SRR4302533.111732/94‑1 (MQ=60)
                                                                             GCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAG            <  SRR4302533.437866/94‑1 (MQ=60)
                                                                             GCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAG            <  SRR4302533.3128325/94‑1 (MQ=60)
                                                                             GCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAG            >  SRR4302533.2260716/1‑94 (MQ=60)
                                                                             GCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAG            <  SRR4302533.5670375/94‑1 (MQ=60)
                                                                                      CACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAGAAAGCACCG   <  SRR4302533.6175873/94‑1 (MQ=60)
                                                                                      CACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAGAAAGCACCG   <  SRR4302533.2642874/94‑1 (MQ=60)
                                                                                       ACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAGAAAGCACCGC  >  SRR4302533.2641024/1‑94 (MQ=60)
                                                                                       |                                                                                             
ATGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATCGTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAGAAAGCACCGC  >  NC_000913/3972554‑3972734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑