Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,008,473 +G intergenic (+40/‑193) b4555 → / → yigM hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,008,4721.G98.9% 561.7 / ‑2.5 180intergenic (+39/‑194)b4555/yigMhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (1/1);  major base G (90/86);  minor base T (1/1);  total (92/88)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.77e-01

Only 100 of 183 total aligned reads displayed.

AACTGGCTACAG‑CTGAAGGATTG‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGG‑A‑AA‑GCTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGC  >  NC_000913/4008399‑4008558
                                                                                 |                                                                                       
actgggCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATggg                                                                            <  1:2656113/89‑1 (MQ=255)
actgggCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATggg                                                                            >  2:5517684/5‑93 (MQ=255)
 ctgggCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGc                                                                           <  1:4035461/90‑1 (MQ=255)
 ctgggCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGc                                                                           <  1:844949/90‑1 (MQ=255)
 ctgggCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGc                                                                           <  2:1450881/90‑1 (MQ=255)
 ctgggCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGc                                                                           >  2:2214314/4‑93 (MQ=255)
  tgggCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCa                                                                          >  1:1750273/3‑93 (MQ=255)
   gggCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAg                                                                         <  2:5597564/92‑1 (MQ=255)
    ggCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGt                                                                        >  1:1525946/1‑93 (MQ=255)
      cTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTgc                                                                      <  2:2350087/93‑2 (MQ=255)
      cTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTgc                                                                      >  1:6162543/1‑92 (MQ=255)
      cTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTgc                                                                      <  2:6200703/93‑2 (MQ=255)
       tACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTgcg                                                                     >  2:2417089/1‑91 (MQ=255)
           gccTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCgcacc                                                                 >  1:1019375/3‑93 (MQ=255)
           gccTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCgcacc                                                                 >  2:3680671/3‑93 (MQ=255)
           gccTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCgcacc                                                                 >  1:5659547/3‑93 (MQ=255)
           gccTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCgcacc                                                                 >  2:398669/3‑93 (MQ=255)
           gccTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCgcacc                                                                 >  1:1553073/3‑93 (MQ=255)
            ccTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCgcacca                                                                >  1:97290/2‑93 (MQ=255)
              tGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGc                                                              >  1:5249945/1‑93 (MQ=255)
              tGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGc                                                              >  2:2309666/1‑93 (MQ=255)
              tGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGc                                                              >  1:1680921/1‑93 (MQ=255)
              tGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGc                                                              >  2:3305289/1‑93 (MQ=255)
              tGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGc                                                              >  2:351796/1‑93 (MQ=255)
               gAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCg                                                             >  1:310058/1‑93 (MQ=255)
               gAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCg                                                             >  2:2994525/1‑93 (MQ=255)
                aaGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGt                                                            <  1:4623016/93‑1 (MQ=255)
                 aGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTc                                                           >  1:4773736/1‑93 (MQ=255)
                  ggATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCt                                                          <  1:5903364/93‑1 (MQ=255)
                               tttggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCAt                                             >  2:4091185/5‑93 (MQ=255)
                               tttggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCAt                                             >  2:3168991/5‑93 (MQ=255)
                                ttggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATc                                            <  2:4996354/90‑1 (MQ=255)
                                ttggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATc                                            <  1:650055/90‑1 (MQ=255)
                                 tggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATcc                                           <  1:1010049/91‑1 (MQ=255)
                                 tggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATcc                                           <  1:2731569/91‑1 (MQ=255)
                                  ggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATccc                                          >  1:1444153/2‑93 (MQ=255)
                                   gtgACGGGATGAACGGCGCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCg                                         <  1:635193/93‑1 (MQ=255)
                                     gACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAg                                       <  1:5351079/93‑1 (MQ=255)
                                     gACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAg                                       <  1:4845784/93‑1 (MQ=255)
                                          gATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑gga                                 >  1:2683761/1‑91 (MQ=255)
                                           aTGAACGACGCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaa                                >  2:5920600/1‑90 (MQ=255)
                                           aTGAACGACGCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaa                                <  2:711517/93‑4 (MQ=255)
                                            tGAACGACGCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaag                               <  1:2003960/93‑5 (MQ=255)
                                            tGAACGACGCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaag                               <  2:1410130/93‑5 (MQ=255)
                                            tGAACGACGCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaag                               >  1:2014182/1‑89 (MQ=255)
                                            tGAACGACGCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaag                               >  1:6081650/1‑89 (MQ=255)
                                              aaCGACGCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTg                              >  2:4270714/1‑93 (MQ=255)
                                              aaCGACGCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTg                              <  1:2064397/93‑1 (MQ=255)
                                               acgacgCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGa                             >  2:1913156/1‑93 (MQ=255)
                                               acgacgCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGa                             >  2:4794832/1‑93 (MQ=255)
                                               acgacgCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGa                             <  2:765178/93‑1 (MQ=255)
                                               acgacgCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGa                             <  1:3184550/93‑1 (MQ=255)
                                               acgacgCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGa                             >  1:4576222/1‑93 (MQ=255)
                                                 gacgCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAtt                           <  1:3473692/93‑1 (MQ=255)
                                                 gacgCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAtt                           >  1:294269/1‑93 (MQ=255)
                                                 gacgCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAtt                           >  1:3576486/1‑93 (MQ=255)
                                                 gacgCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAtt                           <  1:236131/93‑1 (MQ=255)
                                                 gacgCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAtt                           >  2:6052959/1‑93 (MQ=255)
                                                 gacgCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAtt                           <  2:5755891/93‑1 (MQ=255)
                                                  acgCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTg                          >  2:435163/1‑93 (MQ=255)
                                                  acgCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTg                          >  1:313866/1‑93 (MQ=255)
                                                  acgCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTg                          <  1:557163/93‑1 (MQ=255)
                                                  acgCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTg                          >  1:709619/1‑93 (MQ=255)
                                                   cgCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTgg                         <  2:5323175/93‑1 (MQ=255)
                                                   cgCCGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTgg                         <  1:3051728/93‑1 (MQ=255)
                                                     ccGAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTa                       >  2:5347387/1‑93 (MQ=255)
                                                       gAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACt                     >  2:1499851/1‑93 (MQ=255)
                                                       gAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACt                     >  2:5116289/1‑93 (MQ=255)
                                                       gAAATGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTACGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACt                     >  1:3388088/1‑93 (MQ=255)
                                                        aaaTGCTTTCAATGGC‑GGGTA‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTACATCCCGAGCTGGAAGTGATTGGTACTa                    <  2:1426094/93‑1 (MQ=255)
                                                        aaaTGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTa                    >  1:2562391/1‑93 (MQ=255)
                                                        aaaTGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTa                    >  2:4902070/1‑93 (MQ=255)
                                                        aaaTGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTa                    >  1:3810435/1‑93 (MQ=255)
                                                         aaTGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTaa                   >  2:5952502/1‑93 (MQ=255)
                                                         aaTGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTaa                   <  2:5175516/93‑1 (MQ=255)
                                                         aaTGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTaa                   <  2:2978707/93‑1 (MQ=255)
                                                         aaTGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTaa                   <  1:4487952/93‑1 (MQ=255)
                                                         aaTGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTaa                   <  1:1989626/93‑1 (MQ=255)
                                                          aTGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAt                  >  1:1236449/1‑93 (MQ=255)
                                                          aTGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAt                  <  1:5931535/93‑1 (MQ=255)
                                                          aTGCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAt                  <  1:1385819/93‑1 (MQ=255)
                                                            gCTGTCGATGGCGGGG‑A‑AATGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGc                <  1:1074795/93‑1 (MQ=255)
                                                            gCTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGc                >  2:4424484/1‑93 (MQ=255)
                                                             cTGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGcc               <  2:3390358/93‑1 (MQ=255)
                                                              tGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcg              >  2:4774860/1‑93 (MQ=255)
                                                              tGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcg              >  1:3608394/1‑93 (MQ=255)
                                                              tGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcg              >  2:5867509/1‑93 (MQ=255)
                                                              tGTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAGGCcg              >  2:2157613/1‑93 (MQ=255)
                                                               gTCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcga             <  1:3292984/93‑1 (MQ=255)
                                                                tCGATGGCGGGG‑ATAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgac            <  1:2163614/93‑1 (MQ=255)
                                                                tCGATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgac            <  1:5511444/93‑1 (MQ=255)
                                                                  gATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgacga          <  2:38650/93‑1 (MQ=255)
                                                                  gATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgacga          <  2:3320626/93‑1 (MQ=255)
                                                                  gATGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgacga          >  1:4255679/1‑93 (MQ=255)
                                                                   aTGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCgacgac         >  1:6233158/1‑93 (MQ=255)
                                                                   aTGGCGGGG‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCgacgac         <  1:3621465/93‑1 (MQ=255)
                                                                     ggcgggg‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAcgc       <  1:5180493/89‑1 (MQ=255)
                                                                     ggcgggg‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAcgc       <  1:5810938/89‑1 (MQ=255)
                                                                        gggg‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGt    <  2:4813315/92‑1 (MQ=255)
                                                                         ggg‑A‑AAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTg   >  1:3581651/1‑93 (MQ=255)
                                                                                 |                                                                                       
AACTGGCTACAG‑CTGAAGGATTG‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGG‑A‑AA‑GCTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGC  >  NC_000913/4008399‑4008558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGGAAGGCTGGCGAACT‑GGCTACAG‑CTGAAGGATTG‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGG‑‑AAA‑G‑CTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTA  >  NC_000913/4008386‑4008565
                                                                                                |                                                                                              
gcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAA‑G                                                                                                <  SRR4302533.2615802/76‑1 (MQ=53)
       cgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCAT                                                                                         >  SRR4302533.2915384/12‑94 (MQ=49)
       cgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCAT                                                                                         >  SRR4302533.4625322/12‑94 (MQ=49)
        gaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATT                                                                                        >  SRR4302533.3589461/11‑94 (MQ=49)
          agaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTAT                                                                                      <  SRR4302533.2666254/86‑1 (MQ=48)
          agaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTAT                                                                                      >  SRR4302533.3330090/9‑94 (MQ=48)
           gaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATG                                                                                     <  SRR4302533.1052833/87‑1 (MQ=49)
            aaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGG                                                                                    <  SRR4302533.2348022/88‑1 (MQ=51)
            aaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGG                                                                                    <  SRR4302533.4657144/88‑1 (MQ=51)
             AACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAA‑GTCTGCATTATGGG                                                                                   >  SRR4302533.3137206/1‑94 (MQ=48)
             AACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGG                                                                                   <  SRR4302533.1644244/94‑1 (MQ=50)
             AACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGG                                                                                   >  SRR4302533.2705082/1‑94 (MQ=50)
              ACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGC                                                                                  >  SRR4302533.4646378/1‑94 (MQ=53)
              ACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGC                                                                                  <  SRR4302533.2672427/94‑1 (MQ=53)
              ACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGC                                                                                  <  SRR4302533.2005247/94‑1 (MQ=53)
               ctgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCA                                                                                 <  SRR4302533.4060026/91‑1 (MQ=57)
               ctgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCA                                                                                 <  SRR4302533.849893/91‑1 (MQ=57)
               ctgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCA                                                                                 >  SRR4302533.1760930/4‑94 (MQ=57)
                G‑GGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGT                                                                               >  SRR4302533.1535130/1‑94 (MQ=60)
                   GCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGC                                                                             >  SRR4302533.724731/1‑94 (MQ=60)
                   GCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGC                                                                             >  SRR4302533.6199597/1‑94 (MQ=60)
                    CTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGcg                                                                            >  SRR4302533.5783651/1‑92 (MQ=60)
                    CTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGcg                                                                            <  SRR4302533.5572877/94‑3 (MQ=60)
                      ACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGcgca                                                                          <  SRR4302533.6277876/94‑5 (MQ=60)
                        agcCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACC                                                                        <  SRR4302533.5237275/91‑1 (MQ=60)
                        agcCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACC                                                                        >  SRR4302533.1562424/4‑94 (MQ=60)
                        agcCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACC                                                                        >  SRR4302533.5693748/4‑94 (MQ=60)
                        agcCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACC                                                                        >  SRR4302533.1025366/4‑94 (MQ=60)
                         gcCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCA                                                                       <  SRR4302533.3114813/92‑1 (MQ=60)
                         gcCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCA                                                                       >  SRR4302533.97895/3‑94 (MQ=60)
                         C‑CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAG                                                                      >  SRR4302533.2061001/1‑94 (MQ=60)
                           CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAT‑G‑CTGCATTATGGGCAGTGCGCACCAGC                                                                     >  SRR4302533.2649175/1‑94 (MQ=60)
                           CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGC                                                                     >  SRR4302533.5281497/1‑94 (MQ=60)
                           CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGC                                                                     <  SRR4302533.5240768/94‑1 (MQ=60)
                           CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGC                                                                     <  SRR4302533.5893172/94‑1 (MQ=60)
                           CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGC                                                                     >  SRR4302533.1691103/1‑94 (MQ=60)
                           CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGC                                                                     >  SRR4302533.6140799/1‑94 (MQ=60)
                            TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCG                                                                    >  SRR4302533.311955/1‑94 (MQ=57)
                            TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCG                                                                    <  SRR4302533.1754882/94‑1 (MQ=57)
                             GAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGT                                                                   <  SRR4302533.4739807/94‑1 (MQ=57)
                              AAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTC                                                                  <  SRR4302533.4651037/94‑1 (MQ=57)
                              AAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTC                                                                  >  SRR4302533.4802662/1‑94 (MQ=57)
                              AAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTC                                                                  >  SRR4302533.1798484/1‑94 (MQ=57)
                                GGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTG                                                                <  SRR4302533.3707720/94‑1 (MQ=60)
                                GGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTG                                                                >  SRR4302533.5056168/1‑94 (MQ=60)
                                GGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTG                                                                >  SRR4302533.5093451/1‑94 (MQ=60)
                                GGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTG                                                                <  SRR4302533.5939014/94‑1 (MQ=60)
                                 GATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGA                                                               <  SRR4302533.5872538/94‑1 (MQ=60)
                                    tgtattgcgtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAG                                                            <  SRR4302533.6149351/81‑1 (MQ=60)
                                    tgtattgcgtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAG                                                            >  SRR4302533.3367185/14‑94 (MQ=60)
                                    tgtattgcgtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAG                                                            >  SRR4302533.1405984/14‑94 (MQ=60)
                                     gtattgcgtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGA                                                           <  SRR4302533.251519/82‑1 (MQ=60)
                                     gtattgcgtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGA                                                           <  SRR4302533.4321293/82‑1 (MQ=60)
                                     T‑ATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGAC                                                          >  SRR4302533.331590/1‑94 (MQ=60)
                                     T‑ATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGAC                                                          >  SRR4302533.3030052/1‑94 (MQ=60)
                                       ATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACC                                                         >  SRR4302533.3945787/1‑94 (MQ=60)
                                       ATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACC                                                         <  SRR4302533.4518248/94‑1 (MQ=60)
                                          GCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTC                                                      <  SRR4302533.3420724/94‑1 (MQ=60)
                                           CGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCA                                                     >  SRR4302533.4302974/1‑94 (MQ=60)
                                            gtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCAT                                                    <  SRR4302533.4771578/89‑1 (MQ=60)
                                             TG‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCC                                                 >  SRR4302533.1452865/1‑94 (MQ=60)
                                             TG‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCC                                                 <  SRR4302533.1015955/94‑1 (MQ=60)
                                             TG‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCC                                                 <  SRR4302533.2748331/94‑1 (MQ=60)
                                              ttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCC                                                  <  SRR4302533.653891/91‑1 (MQ=60)
                                                 GTGACGGGATGAACGGCGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGA                                               <  SRR4302533.638927/94‑1 (MQ=60)
                                                   GACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC                                             <  SRR4302533.5383189/94‑1 (MQ=60)
                                                   GACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC                                             <  SRR4302533.4875153/94‑1 (MQ=60)
                                                    ACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCG                                            >  SRR4302533.6273983/1‑94 (MQ=60)
                                                     CGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑G                                          <  SRR4302533.654173/94‑1 (MQ=60)
                                                       GGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GGA                                        >  SRR4302533.2700209/1‑94 (MQ=60)
                                                        GATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑Ggaa                                       <  SRR4302533.1163654/94‑4 (MQ=60)
                                                         ATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAG                                       >  SRR4302533.3601632/1‑94 (MQ=60)
                                                         ATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAG                                       >  SRR4302533.422394/1‑94 (MQ=60)
                                                         ATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAG                                       >  SRR4302533.6118427/1‑94 (MQ=60)
                                                         ATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAG                                       >  SRR4302533.2026590/1‑94 (MQ=60)
                                                          TGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGT                                      <  SRR4302533.2016301/94‑1 (MQ=60)
                                                          TGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGT                                      <  SRR4302533.2112503/94‑1 (MQ=60)
                                                          TGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑Gggagt                                     >  SRR4302533.2259585/1‑89 (MQ=60)
                                                           GAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTG                                     <  SRR4302533.404968/94‑1 (MQ=60)
                                                            AACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGA                                    <  SRR4302533.2077121/94‑1 (MQ=60)
                                                            AACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGA                                    >  SRR4302533.4603957/1‑94 (MQ=60)
                                                             ACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAT                                   <  SRR4302533.3204137/94‑1 (MQ=60)
                                                              CGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATT                                  >  SRR4302533.3598527/1‑94 (MQ=60)
                                                              CGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATT                                  >  SRR4302533.296066/1‑94 (MQ=60)
                                                               GACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGGTTG                                 >  SRR4302533.5622187/1‑94 (MQ=60)
                                                               GACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTG                                 <  SRR4302533.4854994/94‑1 (MQ=60)
                                                               GACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTG                                 <  SRR4302533.6030519/94‑1 (MQ=60)
                                                               GACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTG                                 <  SRR4302533.237587/94‑1 (MQ=60)
                                                               GACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTG                                 <  SRR4302533.2760624/94‑1 (MQ=60)
                                                               GACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTG                                 >  SRR4302533.315784/1‑94 (MQ=60)
                                                               GACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTG                                 >  SRR4302533.713810/1‑94 (MQ=60)
                                                               GACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTG                                 <  SRR4302533.3495127/94‑1 (MQ=60)
                                                                ACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGG                                <  SRR4302533.560469/94‑1 (MQ=60)
                                                                 CGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGT                               <  SRR4302533.3070417/94‑1 (MQ=60)
                                                                 CGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGT                               <  SRR4302533.3903849/94‑1 (MQ=60)
                                                                  GCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTA                              <  SRR4302533.2509245/94‑1 (MQ=60)
                                                                  GCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTA                              >  SRR4302533.3587940/1‑94 (MQ=60)
                                                                  GCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTA                              <  SRR4302533.3334030/94‑1 (MQ=60)
                                                                    CGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTACGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACT                            >  SRR4302533.3409008/1‑94 (MQ=60)
                                                                     GAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTA                           >  SRR4302533.3833740/1‑94 (MQ=60)
                                                                     GAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTA                           >  SRR4302533.5033191/1‑94 (MQ=60)
                                                                     GAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTA                           >  SRR4302533.2578166/1‑94 (MQ=60)
                                                                      AAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAA                          <  SRR4302533.2340893/94‑1 (MQ=60)
                                                                       AATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAT                         >  SRR4302533.1066069/1‑94 (MQ=60)
                                                                       AATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAT                         <  SRR4302533.2001805/94‑1 (MQ=60)
                                                                       AATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAT                         >  SRR4302533.1243837/1‑94 (MQ=60)
                                                                       AATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAT                         <  SRR4302533.4515122/94‑1 (MQ=60)
                                                                       AATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAT                         >  SRR4302533.979437/1‑94 (MQ=60)
                                                                        ATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATG                        >  SRR4302533.4382678/1‑94 (MQ=60)
                                                                        ATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATG                        <  SRR4302533.5967395/94‑1 (MQ=60)
                                                                        ATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATG                        <  SRR4302533.1394158/94‑1 (MQ=60)
                                                                          GCTGTCGATGGC‑GGGGAAAT‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCC                      <  SRR4302533.1081131/94‑1 (MQ=60)
                                                                           CTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                     >  SRR4302533.3630606/1‑94 (MQ=60)
                                                                           CTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                     >  SRR4302533.1585259/1‑94 (MQ=60)
                                                                            TGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGA                    <  SRR4302533.872027/94‑1 (MQ=60)
                                                                             GTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGAC                   <  SRR4302533.5462091/94‑1 (MQ=60)
                                                                             GTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGAC                   <  SRR4302533.3313310/94‑1 (MQ=60)
                                                                              TCGATGGC‑GGGGATAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACG                  <  SRR4302533.2176926/94‑1 (MQ=60)
                                                                              TCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACG                  <  SRR4302533.5544718/94‑1 (MQ=60)
                                                                               CGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGA                 >  SRR4302533.4281337/1‑94 (MQ=60)
                                                                               CGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGA                 >  SRR4302533.3244615/1‑94 (MQ=60)
                                                                                GATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAC                >  SRR4302533.3040421/1‑94 (MQ=60)
                                                                                GATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAC                <  SRR4302533.1536061/94‑1 (MQ=60)
                                                                                GATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAC                >  SRR4302533.6270481/1‑94 (MQ=60)
                                                                                GATGGC‑GGGGAAAG‑G‑CTGCATGATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAC                <  SRR4302533.1434815/94‑1 (MQ=60)
                                                                                 ATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACG               <  SRR4302533.3643750/94‑1 (MQ=60)
                                                                                 ATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACG               <  SRR4302533.6248425/94‑1 (MQ=60)
                                                                                 ATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACG               >  SRR4302533.4061225/1‑94 (MQ=60)
                                                                                   GGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCG             <  SRR4302533.5846030/94‑1 (MQ=60)
                                                                                   GGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCG             <  SRR4302533.5211778/94‑1 (MQ=60)
                                                                                   GGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCG             <  SRR4302533.3079970/94‑1 (MQ=60)
                                                                                   GGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCG             <  SRR4302533.3415015/94‑1 (MQ=60)
                                                                                       cggggaaagG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGG           >  SRR4302533.1350443/10‑94 (MQ=60)
                                                                                       GGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGCGATTGGTACTAATGCCGACGACGCGGTGC         >  SRR4302533.3640954/1‑94 (MQ=60)
                                                                                        ggggaaagG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTG          >  SRR4302533.3603714/9‑94 (MQ=60)
                                                                                        GG‑‑AAAGG‑CTGCATTATGGGCAGTTCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACCACGCGGTGCC        >  SRR4302533.1525870/1‑94 (MQ=60)
                                                                                        GG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCC        >  SRR4302533.512215/1‑94 (MQ=60)
                                                                                         G‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCG       >  SRR4302533.4097476/1‑94 (MQ=60)
                                                                                            AAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCA     <  SRR4302533.4998114/94‑1 (MQ=60)
                                                                                             AG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAT    >  SRR4302533.2369003/1‑94 (MQ=60)
                                                                                             AG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAT    >  SRR4302533.3878084/1‑94 (MQ=60)
                                                                                                G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTA  <  SRR4302533.2690988/94‑1 (MQ=60)
                                                                                                G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTA  <  SRR4302533.634448/94‑1 (MQ=60)
                                                                                                |                                                                                              
TGGAAGGCTGGCGAACT‑GGCTACAG‑CTGAAGGATTG‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGG‑‑AAA‑G‑CTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTA  >  NC_000913/4008386‑4008565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑