Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,008,491 2 bp→CG intergenic (+58/‑174) b4555 → / → yigM hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,008,4910GC100.0% 627.8 / NA 196intergenic (+58/‑175)b4555/yigMhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (100/96);  total (100/96)
*NC_0009134,008,4920CG100.0% 610.9 / NA 190intergenic (+59/‑174)b4555/yigMhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (94/96);  total (94/96)

Only 100 of 200 total aligned reads displayed.

AGCTGAAGGATTG‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑AAA‑GCTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACT  >  NC_000913/4008409‑4008577
                                                                                         ||                                                                                      
gccTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCgcacc                                                                                    >  2:3680671/3‑93 (MQ=255)
 ccTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCgcacca                                                                                   >  1:97290/2‑93 (MQ=255)
   tGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGc                                                                                 >  1:5249945/1‑93 (MQ=255)
   tGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGc                                                                                 >  1:6103955/1‑93 (MQ=255)
   tGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGc                                                                                 <  1:1744278/93‑1 (MQ=255)
   tGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGc                                                                                 <  2:3465136/93‑1 (MQ=255)
    gAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGG‑AAG‑GCTGCATTATGGGCAGGGCGCACCACCg                                                                                >  2:5814954/1‑93 (MQ=255)
    gAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCg                                                                                >  1:310058/1‑93 (MQ=255)
    gAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCg                                                                                <  1:4711253/93‑1 (MQ=255)
    gAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCg                                                                                >  2:2994525/1‑93 (MQ=255)
     aaGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGt                                                                               <  1:4623016/93‑1 (MQ=255)
       ggATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCt                                                                             <  1:5903364/93‑1 (MQ=255)
                    tttggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCAt                                                                >  2:3168991/5‑93 (MQ=255)
                    tttggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCAt                                                                >  2:4091185/5‑93 (MQ=255)
                     ttggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATc                                                               <  1:650055/90‑1 (MQ=255)
                      tggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATcc                                                              <  1:2731569/91‑1 (MQ=255)
                      tggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATcc                                                              <  1:1010049/91‑1 (MQ=255)
                       ggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATccc                                                             <  2:3869813/92‑1 (MQ=255)
                          gACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAg                                                          <  1:5351079/93‑1 (MQ=255)
                              ggATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑gg                                                     >  2:4893182/1‑92 (MQ=255)
                              ggATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGAAGTGCGCACCAGCGTCTGAAAGACCTTCATCCTGAGCT‑gg                                                     >  2:4325498/1‑92 (MQ=255)
                               gATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑gga                                                    <  1:1156714/93‑3 (MQ=255)
                                aTGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaa                                                   >  2:4231908/1‑90 (MQ=255)
                                aTGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaa                                                   <  2:711517/93‑4 (MQ=255)
                                aTGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaa                                                   <  2:5543605/93‑4 (MQ=255)
                                aTGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaa                                                   >  2:4369666/1‑90 (MQ=255)
                                 tGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaag                                                  >  1:2014182/1‑89 (MQ=255)
                                 tGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaag                                                  <  1:2099551/93‑5 (MQ=255)
                                 tGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaag                                                  <  2:1410130/93‑5 (MQ=255)
                                 tGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaag                                                  >  1:419879/1‑89 (MQ=255)
                                   aaCGACGCCGAAATGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTg                                                 <  1:2064397/93‑1 (MQ=255)
                                   aaCGACGCCGAAATGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTg                                                 >  2:674673/1‑93 (MQ=255)
                                    acgacgCCGAAATGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGa                                                <  1:3184550/93‑1 (MQ=255)
                                    acgacgCCGAAATGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGa                                                <  2:765178/93‑1 (MQ=255)
                                    acgacgCCGAAATGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGa                                                >  2:4794832/1‑93 (MQ=255)
                                     cgacgCCGAAATGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCTGTGCGCACCAGCGTCTGAAAGAGCTTCATCCCGAGCTGGAAGTGAt                                               <  2:5071661/93‑1 (MQ=255)
                                      gacgCCGAAATGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAtt                                              <  1:4825751/93‑1 (MQ=255)
                                      gacgCCGAAATGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAtt                                              >  1:3576486/1‑93 (MQ=255)
                                      gacgCCGAAATGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAtt                                              >  2:5321920/1‑93 (MQ=255)
                                      gacgCCGAAATGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAtt                                              <  1:236131/93‑1 (MQ=255)
                                      gacgCCGAAATGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAtt                                              >  2:6052959/1‑93 (MQ=255)
                                      gacgCCGAAATGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAtt                                              <  1:5994215/93‑1 (MQ=255)
                                       acgCCGAAATGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGGTTg                                             >  1:5588423/1‑93 (MQ=255)
                                       acgCCGAAATGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTg                                             >  1:709619/1‑93 (MQ=255)
                                        cgCCGAAATGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTgg                                            <  1:3051728/93‑1 (MQ=255)
                                          ccGAAATGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTa                                          >  2:5347387/1‑93 (MQ=255)
                                            gAAATGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACt                                        >  2:1499851/1‑93 (MQ=255)
                                            gAAATGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTACGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACt                                        >  1:3388088/1‑93 (MQ=255)
                                             aaaTGCTTTCAATGGC‑‑GGGTAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTACATCCCGAGCTGGAAGTGATTGGTACTa                                       <  2:1426094/93‑1 (MQ=255)
                                             aaaTGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTa                                       <  1:2326490/93‑1 (MQ=255)
                                             aaaTGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTa                                       >  1:2562391/1‑93 (MQ=255)
                                             aaaTGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTa                                       >  2:4967993/1‑93 (MQ=255)
                                              aaTGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTaa                                      <  1:1989626/93‑1 (MQ=255)
                                              aaTGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTaa                                      >  2:5952502/1‑93 (MQ=255)
                                              aaTGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTaa                                      <  1:4487952/93‑1 (MQ=255)
                                              aaTGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTaa                                      <  2:2978707/93‑1 (MQ=255)
                                              aaTGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTaa                                      <  2:5175516/93‑1 (MQ=255)
                                               aTGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAt                                     >  1:1059838/1‑93 (MQ=255)
                                               aTGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAt                                     <  2:2585033/93‑1 (MQ=255)
                                                tGCTGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATg                                    >  1:4356384/1‑93 (MQ=255)
                                                   tGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcg                                 >  2:4774860/1‑93 (MQ=255)
                                                   tGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcg                                 >  1:1575772/1‑93 (MQ=255)
                                                   tGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcg                                 >  2:5867509/1‑93 (MQ=255)
                                                   tGTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAGGCcg                                 >  2:2157613/1‑93 (MQ=255)
                                                    gTCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcga                                <  1:3292984/93‑1 (MQ=255)
                                                     tCGATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgac                               <  1:5511444/93‑1 (MQ=255)
                                                       gATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgacga                             >  2:447040/1‑93 (MQ=255)
                                                       gATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgacga                             <  1:1526869/93‑1 (MQ=255)
                                                       gATGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgacga                             <  2:3320626/93‑1 (MQ=255)
                                                       gATGGCG‑GGG‑AAAGGCTGCATGATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgacga                             <  1:1426193/93‑1 (MQ=255)
                                                        aTGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCgacgac                            >  1:3021932/1‑93 (MQ=255)
                                                        aTGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCgacgac                            <  1:3621465/93‑1 (MQ=255)
                                                        aTGGCG‑GGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCgacgac                            >  1:6233158/1‑93 (MQ=255)
                                                           ggcgggg‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAcgc                          <  1:5810938/89‑1 (MQ=255)
                                                           ggcgggg‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAcgc                          <  1:5180493/89‑1 (MQ=255)
                                                           ggcgggg‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGACGACGAcgc                          >  2:337178/5‑93 (MQ=255)
                                                             cgggg‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCgg                        <  2:2279895/91‑1 (MQ=255)
                                                              gggg‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCggg                       >  1:1342361/2‑92 (MQ=255)
                                                               ggg‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTg                      >  1:3581651/1‑93 (MQ=255)
                                                                gg‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGCGATTGGTACTAATGCCGACGACGCGGTGc                     >  1:3618696/1‑93 (MQ=255)
                                                                   aaaggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCg                   >  1:4072682/5‑93 (MQ=255)
                                                                   aaaggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCg                   >  2:24439/5‑93 (MQ=255)
                                                                    aaggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGc                  <  1:4967993/90‑1 (MQ=255)
                                                                      ggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAt                >  1:3854543/2‑93 (MQ=255)
                                                                       gCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAtt               <  1:2674585/93‑1 (MQ=255)
                                                                       gCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAtt               <  1:630739/93‑1 (MQ=255)
                                                                        cTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTa              <  1:5683007/93‑1 (MQ=255)
                                                                         tGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAt             <  2:1442960/93‑1 (MQ=255)
                                                                         tGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAt             <  2:4817850/93‑1 (MQ=255)
                                                                         tGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATACCGACGACGCGGTGCCGCATTAt             >  2:5721900/1‑93 (MQ=255)
                                                                          gCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATc            <  2:3735542/93‑1 (MQ=255)
                                                                           cATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCt           >  1:3558485/1‑93 (MQ=255)
                                                                             ttATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGc         >  2:1550013/1‑93 (MQ=255)
                                                                             ttATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGc         <  2:2877596/93‑1 (MQ=255)
                                                                              tATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCg        <  1:2923090/93‑1 (MQ=255)
                                                                              tATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCg        >  2:1077823/1‑93 (MQ=255)
                                                                                tGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTa      >  2:5128465/1‑93 (MQ=255)
                                                                                  ggCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaaa    <  2:4041485/93‑1 (MQ=255)
                                                                                   gCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAAc   <  2:1704532/93‑1 (MQ=255)
                                                                                    cAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAAct  >  1:3433719/1‑93 (MQ=255)
                                                                                         ||                                                                                      
AGCTGAAGGATTG‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑AAA‑GCTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACT  >  NC_000913/4008409‑4008577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCTACAG‑CTGAAGGATTG‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑‑AAA‑GCTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCT  >  NC_000913/4008404‑4008579
                                                                                              |                                                                                         
GCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGC                                                                                           >  SRR4302533.724731/1‑94 (MQ=60)
GCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGC                                                                                           >  SRR4302533.6199597/1‑94 (MQ=60)
     agcCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACC                                                                                      >  SRR4302533.1025366/4‑94 (MQ=60)
     agcCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACC                                                                                      >  SRR4302533.1562424/4‑94 (MQ=60)
     agcCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACC                                                                                      >  SRR4302533.5693748/4‑94 (MQ=60)
     agcCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACC                                                                                      <  SRR4302533.5237275/91‑1 (MQ=60)
      gcCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCA                                                                                     >  SRR4302533.97895/3‑94 (MQ=60)
      gcCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCA                                                                                     <  SRR4302533.3114813/92‑1 (MQ=60)
      C‑CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAG                                                                                    >  SRR4302533.2061001/1‑94 (MQ=60)
        CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAT‑GCTGCATTATGGGCAGTGCGCACCAGC                                                                                   >  SRR4302533.2649175/1‑94 (MQ=60)
        CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGC                                                                                   <  SRR4302533.5240768/94‑1 (MQ=60)
        CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGC                                                                                   >  SRR4302533.5281497/1‑94 (MQ=60)
        CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGC                                                                                   <  SRR4302533.5893172/94‑1 (MQ=60)
        CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGC                                                                                   >  SRR4302533.1691103/1‑94 (MQ=60)
        CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGC                                                                                   >  SRR4302533.6140799/1‑94 (MQ=60)
         TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCG                                                                                  <  SRR4302533.1754882/94‑1 (MQ=57)
         TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCG                                                                                  >  SRR4302533.311955/1‑94 (MQ=57)
          GAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGT                                                                                 <  SRR4302533.4739807/94‑1 (MQ=57)
           AAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTC                                                                                >  SRR4302533.1798484/1‑94 (MQ=57)
           AAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTC                                                                                >  SRR4302533.4802662/1‑94 (MQ=57)
           AAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTC                                                                                <  SRR4302533.4651037/94‑1 (MQ=57)
             GGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTG                                                                              >  SRR4302533.5093451/1‑94 (MQ=60)
             GGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTG                                                                              >  SRR4302533.5056168/1‑94 (MQ=60)
             GGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTG                                                                              <  SRR4302533.5939014/94‑1 (MQ=60)
             GGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTG                                                                              <  SRR4302533.3707720/94‑1 (MQ=60)
              GATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGA                                                                             <  SRR4302533.5872538/94‑1 (MQ=60)
                 tgtattgcgtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAG                                                                          >  SRR4302533.3367185/14‑94 (MQ=60)
                 tgtattgcgtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAG                                                                          <  SRR4302533.6149351/81‑1 (MQ=60)
                 tgtattgcgtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAG                                                                          >  SRR4302533.1405984/14‑94 (MQ=60)
                  gtattgcgtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGA                                                                         <  SRR4302533.251519/82‑1 (MQ=60)
                  gtattgcgtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGA                                                                         <  SRR4302533.4321293/82‑1 (MQ=60)
                  T‑ATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGAC                                                                        >  SRR4302533.331590/1‑94 (MQ=60)
                  T‑ATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGAC                                                                        >  SRR4302533.3030052/1‑94 (MQ=60)
                    ATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACC                                                                       >  SRR4302533.3945787/1‑94 (MQ=60)
                    ATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACC                                                                       <  SRR4302533.4518248/94‑1 (MQ=60)
                       GCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTC                                                                    <  SRR4302533.3420724/94‑1 (MQ=60)
                        CGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCA                                                                   >  SRR4302533.4302974/1‑94 (MQ=60)
                         gtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCAT                                                                  <  SRR4302533.4771578/89‑1 (MQ=60)
                          TG‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCC                                                               >  SRR4302533.1452865/1‑94 (MQ=60)
                          TG‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCC                                                               <  SRR4302533.1015955/94‑1 (MQ=60)
                          TG‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCC                                                               <  SRR4302533.2748331/94‑1 (MQ=60)
                           ttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCC                                                                <  SRR4302533.653891/91‑1 (MQ=60)
                              GTGACGGGATGAACGGCGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGA                                                             <  SRR4302533.638927/94‑1 (MQ=60)
                                GACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC                                                           <  SRR4302533.5383189/94‑1 (MQ=60)
                                GACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC                                                           <  SRR4302533.4875153/94‑1 (MQ=60)
                                 ACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCG                                                          >  SRR4302533.6273983/1‑94 (MQ=60)
                                  CGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑G                                                        <  SRR4302533.654173/94‑1 (MQ=60)
                                    GGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GGA                                                      >  SRR4302533.2700209/1‑94 (MQ=60)
                                     GATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑Ggaa                                                     <  SRR4302533.1163654/94‑4 (MQ=60)
                                      ATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAG                                                     >  SRR4302533.2026590/1‑94 (MQ=60)
                                      ATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAG                                                     >  SRR4302533.6118427/1‑94 (MQ=60)
                                      ATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAG                                                     >  SRR4302533.422394/1‑94 (MQ=60)
                                      ATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAG                                                     >  SRR4302533.3601632/1‑94 (MQ=60)
                                       TGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGT                                                    <  SRR4302533.2016301/94‑1 (MQ=60)
                                       TGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGT                                                    <  SRR4302533.2112503/94‑1 (MQ=60)
                                       TGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑Gggagt                                                   >  SRR4302533.2259585/1‑89 (MQ=60)
                                        GAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTG                                                   <  SRR4302533.404968/94‑1 (MQ=60)
                                         AACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGA                                                  <  SRR4302533.2077121/94‑1 (MQ=60)
                                         AACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGA                                                  >  SRR4302533.4603957/1‑94 (MQ=60)
                                          ACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAT                                                 <  SRR4302533.3204137/94‑1 (MQ=60)
                                           CGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATT                                                >  SRR4302533.3598527/1‑94 (MQ=60)
                                           CGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATT                                                >  SRR4302533.296066/1‑94 (MQ=60)
                                            GACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGGTTG                                               >  SRR4302533.5622187/1‑94 (MQ=60)
                                            GACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTG                                               <  SRR4302533.2760624/94‑1 (MQ=60)
                                            GACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTG                                               >  SRR4302533.315784/1‑94 (MQ=60)
                                            GACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTG                                               <  SRR4302533.4854994/94‑1 (MQ=60)
                                            GACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTG                                               <  SRR4302533.6030519/94‑1 (MQ=60)
                                            GACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTG                                               <  SRR4302533.237587/94‑1 (MQ=60)
                                            GACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTG                                               <  SRR4302533.3495127/94‑1 (MQ=60)
                                            GACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTG                                               >  SRR4302533.713810/1‑94 (MQ=60)
                                             ACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGG                                              <  SRR4302533.560469/94‑1 (MQ=60)
                                              CGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGT                                             <  SRR4302533.3903849/94‑1 (MQ=60)
                                              CGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGT                                             <  SRR4302533.3070417/94‑1 (MQ=60)
                                               GCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTA                                            <  SRR4302533.2509245/94‑1 (MQ=60)
                                               GCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTA                                            <  SRR4302533.3334030/94‑1 (MQ=60)
                                               GCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTA                                            >  SRR4302533.3587940/1‑94 (MQ=60)
                                                 CGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTACGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACT                                          >  SRR4302533.3409008/1‑94 (MQ=60)
                                                  GAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTA                                         >  SRR4302533.5033191/1‑94 (MQ=60)
                                                  GAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTA                                         >  SRR4302533.2578166/1‑94 (MQ=60)
                                                  GAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTA                                         >  SRR4302533.3833740/1‑94 (MQ=60)
                                                   AAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAA                                        <  SRR4302533.2340893/94‑1 (MQ=60)
                                                    AATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAT                                       >  SRR4302533.979437/1‑94 (MQ=60)
                                                    AATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAT                                       >  SRR4302533.1066069/1‑94 (MQ=60)
                                                    AATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAT                                       <  SRR4302533.4515122/94‑1 (MQ=60)
                                                    AATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAT                                       <  SRR4302533.2001805/94‑1 (MQ=60)
                                                    AATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAT                                       >  SRR4302533.1243837/1‑94 (MQ=60)
                                                     ATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATG                                      >  SRR4302533.4382678/1‑94 (MQ=60)
                                                     ATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATG                                      <  SRR4302533.1394158/94‑1 (MQ=60)
                                                     ATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATG                                      <  SRR4302533.5967395/94‑1 (MQ=60)
                                                       GCTGTCGATGGCGGGGAAAT‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCC                                    <  SRR4302533.1081131/94‑1 (MQ=60)
                                                        CTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                                   >  SRR4302533.1585259/1‑94 (MQ=60)
                                                        CTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                                   >  SRR4302533.3630606/1‑94 (MQ=60)
                                                         TGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGA                                  <  SRR4302533.872027/94‑1 (MQ=60)
                                                          GTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGAC                                 <  SRR4302533.3313310/94‑1 (MQ=60)
                                                          GTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGAC                                 <  SRR4302533.5462091/94‑1 (MQ=60)
                                                           TCGATGGCGGGGATAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACG                                <  SRR4302533.2176926/94‑1 (MQ=60)
                                                           TCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACG                                <  SRR4302533.5544718/94‑1 (MQ=60)
                                                            CGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGA                               >  SRR4302533.3244615/1‑94 (MQ=60)
                                                            CGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGA                               >  SRR4302533.4281337/1‑94 (MQ=60)
                                                             GATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAC                              <  SRR4302533.1536061/94‑1 (MQ=60)
                                                             GATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAC                              >  SRR4302533.6270481/1‑94 (MQ=60)
                                                             GATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAC                              >  SRR4302533.3040421/1‑94 (MQ=60)
                                                             GATGGCGGGGAAAG‑GCTGCATGATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAC                              <  SRR4302533.1434815/94‑1 (MQ=60)
                                                              ATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACG                             >  SRR4302533.4061225/1‑94 (MQ=60)
                                                              ATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACG                             <  SRR4302533.6248425/94‑1 (MQ=60)
                                                              ATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACG                             <  SRR4302533.3643750/94‑1 (MQ=60)
                                                                GGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCG                           <  SRR4302533.5211778/94‑1 (MQ=60)
                                                                GGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCG                           <  SRR4302533.3079970/94‑1 (MQ=60)
                                                                GGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCG                           <  SRR4302533.3415015/94‑1 (MQ=60)
                                                                GGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCG                           <  SRR4302533.5846030/94‑1 (MQ=60)
                                                                   cggggaaagGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGG                         >  SRR4302533.1350443/10‑94 (MQ=60)
                                                                   GGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGCGATTGGTACTAATGCCGACGACGCGGTGC                       >  SRR4302533.3640954/1‑94 (MQ=60)
                                                                    ggggaaagGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTG                        >  SRR4302533.3603714/9‑94 (MQ=60)
                                                                    GG‑‑AAAGGCTGCATTATGGGCAGTTCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACCACGCGGTGCC                      >  SRR4302533.1525870/1‑94 (MQ=60)
                                                                    GG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCC                      >  SRR4302533.512215/1‑94 (MQ=60)
                                                                     G‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCG                     >  SRR4302533.4097476/1‑94 (MQ=60)
                                                                        AAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCA                   <  SRR4302533.4998114/94‑1 (MQ=60)
                                                                         AG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAT                  >  SRR4302533.3878084/1‑94 (MQ=60)
                                                                         AG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAT                  >  SRR4302533.2369003/1‑94 (MQ=60)
                                                                            GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTA                <  SRR4302533.2690988/94‑1 (MQ=60)
                                                                            GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTA                <  SRR4302533.634448/94‑1 (MQ=60)
                                                                             CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAT               >  SRR4302533.4185549/1‑94 (MQ=60)
                                                                             CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAT               <  SRR4302533.5717337/94‑1 (MQ=60)
                                                                              TGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATC              <  SRR4302533.5346181/94‑1 (MQ=60)
                                                                               GCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCT             <  SRR4302533.4736611/94‑1 (MQ=60)
                                                                               GCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCT             >  SRR4302533.3580413/1‑94 (MQ=60)
                                                                               GCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATGGGTACTAATGCCGACGACGCGGTGCCGCATTATCT             <  SRR4302533.4412930/94‑1 (MQ=60)
                                                                                CATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTG            <  SRR4302533.2082373/94‑1 (MQ=60)
                                                                                  TTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCG          >  SRR4302533.3054878/1‑94 (MQ=60)
                                                                                   TATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGT         <  SRR4302533.2940988/94‑1 (MQ=60)
                                                                                    ATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTA        >  SRR4302533.5719269/1‑94 (MQ=60)
                                                                                        GCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACT    >  SRR4302533.3454933/1‑94 (MQ=60)
                                                                                         CAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTC   >  SRR4302533.3777504/1‑94 (MQ=60)
                                                                                          AGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACTACGCGGTGCCGCATTATCTGCGTAAACTCT  <  SRR4302533.2624621/94‑1 (MQ=60)
                                                                                          AGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCT  >  SRR4302533.892202/1‑94 (MQ=60)
                                                                                          AGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCT  <  SRR4302533.4790295/94‑1 (MQ=60)
                                                                                              |                                                                                         
GCTACAG‑CTGAAGGATTG‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑‑AAA‑GCTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCT  >  NC_000913/4008404‑4008579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑