Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,013,772 +C intergenic (‑53/‑246) ysgA ← / → udp hypothetical protein/uridine phosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,013,7701.C99.1% 691.6 / ‑1.2 215intergenic (‑51/‑248)ysgA/udphypothetical protein/uridine phosphorylase
Reads supporting (aligned to +/- strand):  ref base . (0/2);  new base C (104/109);  total (104/111)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.75e-01

Only 100 of 234 total aligned reads displayed.

GCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGC‑ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTG  >  NC_000913/4013679‑4013857
                                                                                             |                                                                                       
gcgTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTa                                                                                          >  1:4520248/1‑93 (MQ=255)
  gtcgtcCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑cc                                                                                       >  2:5755551/1‑93 (MQ=255)
   tcgtcCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑ccc                                                                                      <  2:3988178/93‑2 (MQ=255)
      tcCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑ccctta                                                                                   <  1:1493378/93‑5 (MQ=255)
      tcCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑ccctta                                                                                   >  1:915033/1‑89 (MQ=255)
        cGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTtata                                                                                  >  2:4959555/1‑93 (MQ=255)
        cGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTtata                                                                                  >  2:1096075/1‑93 (MQ=255)
           ggTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATaaaa                                                                               >  1:6240077/1‑93 (MQ=255)
            gTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATaaaaa                                                                              <  1:5328840/93‑1 (MQ=255)
            gTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATaaaaa                                                                              >  2:5829914/1‑93 (MQ=255)
             tCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAAt                                                                             >  2:4790467/1‑93 (MQ=255)
             tCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAAt                                                                             <  2:2825021/93‑1 (MQ=255)
              cTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATg                                                                            >  1:4407772/1‑93 (MQ=255)
              cTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATg                                                                            >  1:2632840/1‑93 (MQ=255)
               tGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGt                                                                           >  1:964059/1‑93 (MQ=255)
                gAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGtt                                                                          >  2:3494040/1‑93 (MQ=255)
                 aaCGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTCCCCTTATAAAAATGTTg                                                                         <  1:2800959/93‑1 (MQ=255)
                 aaCGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTg                                                                         <  2:5636082/93‑1 (MQ=255)
                 aaCGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTg                                                                         >  1:4878047/1‑93 (MQ=255)
                 aaCGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTg                                                                         <  1:1234062/93‑1 (MQ=255)
                  aCGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTgc                                                                        >  2:3191796/1‑93 (MQ=255)
                  aCGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTgc                                                                        <  1:5755426/93‑1 (MQ=255)
                   cGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTgcg                                                                       >  1:4103263/1‑93 (MQ=255)
                   cGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTgcg                                                                       >  2:3456413/1‑93 (MQ=255)
                     aTGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCa                                                                     >  1:1028847/1‑93 (MQ=255)
                      tGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCaa                                                                    <  2:5555360/93‑1 (MQ=255)
                         tCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGt                                                                 <  2:1568641/93‑1 (MQ=255)
                          cGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGtt                                                                <  2:5169311/93‑1 (MQ=255)
                          cGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGtt                                                                <  2:4881143/93‑1 (MQ=255)
                          cGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGtt                                                                <  1:1748760/93‑1 (MQ=255)
                           gAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTa                                                               >  1:3548567/1‑93 (MQ=255)
                             aGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAAc                                                             >  1:2222562/1‑93 (MQ=255)
                               cGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACta                                                           >  2:430227/1‑93 (MQ=255)
                                 agagGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACtata                                                         >  1:5068678/1‑93 (MQ=255)
                                 agagGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACtata                                                         <  1:801822/93‑1 (MQ=255)
                                 agagGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACtata                                                         >  1:1678927/1‑93 (MQ=255)
                                 agagGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACtata                                                         <  2:223745/93‑1 (MQ=255)
                                    ggcgaTGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTc                                                      <  2:2760932/89‑1 (MQ=255)
                                    ggcgaTGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTc                                                      <  2:4349040/89‑1 (MQ=255)
                                     gcgaTGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCa                                                     <  2:1123093/90‑1 (MQ=255)
                                     gcgaTGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCa                                                     <  2:4405018/90‑1 (MQ=255)
                                     gcgaTGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCa                                                     <  2:4531841/90‑1 (MQ=255)
                                      cgaTGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAg                                                    <  1:3299934/91‑1 (MQ=255)
                                       gaTGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGc                                                   >  1:6110375/2‑93 (MQ=255)
                                       gaTGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGc                                                   >  1:77129/2‑93 (MQ=255)
                                       gaTGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGc                                                   >  1:2524655/2‑93 (MQ=255)
                                        aTGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCa                                                  >  2:1422140/1‑93 (MQ=255)
                                        aTGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCa                                                  >  2:509310/1‑93 (MQ=255)
                                        aTGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCa                                                  <  2:5295883/93‑1 (MQ=255)
                                        aTGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGGATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCa                                                  >  1:2380867/1‑93 (MQ=255)
                                         tGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCAt                                                 <  1:4684344/93‑1 (MQ=255)
                                         tGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCAt                                                 <  2:4622057/93‑1 (MQ=255)
                                         tGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCAt                                                 <  1:1374003/93‑1 (MQ=255)
                                          gcagcaGGTGCAAATCCAGATTGTAGTGTTGTTGCCATGTTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATg                                                <  1:6080080/93‑1 (MQ=255)
                                           cagcagGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGc                                               >  1:1907584/1‑93 (MQ=255)
                                            agcagGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGca                                              >  2:6233065/1‑93 (MQ=255)
                                             gcagGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGcaa                                             >  2:3715507/1‑93 (MQ=255)
                                             gcagGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGcaa                                             >  2:341098/1‑93 (MQ=255)
                                             gcagGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGcaa                                             >  2:2106399/1‑93 (MQ=255)
                                              cagGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGcaac                                            <  2:1300727/93‑1 (MQ=255)
                                                 gTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACaaa                                         <  1:5805136/93‑1 (MQ=255)
                                                 gTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACaaa                                         <  1:5245090/93‑1 (MQ=255)
                                                  tGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAAt                                        >  2:2183224/1‑93 (MQ=255)
                                                  tGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAAt                                        <  2:461053/93‑1 (MQ=255)
                                                   gCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATc                                       <  2:2581664/93‑1 (MQ=255)
                                                    cAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATca                                      <  2:1867373/93‑1 (MQ=255)
                                                    cAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATca                                      <  2:6194631/93‑1 (MQ=255)
                                                      aaTCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATcaca                                    <  2:1179637/93‑1 (MQ=255)
                                                      aaTCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATcaca                                    <  2:3298290/93‑1 (MQ=255)
                                                        tCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACAtt                                  <  1:5843890/93‑1 (MQ=255)
                                                        tCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACAtt                                  <  2:2969071/93‑1 (MQ=255)
                                                        tCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACAtt                                  <  1:538958/93‑1 (MQ=255)
                                                         ccAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACAttc                                 >  1:3731758/1‑92 (MQ=255)
                                                         ccAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTg                                 >  1:2515875/1‑93 (MQ=255)
                                                          cAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGc                                >  1:4955515/1‑93 (MQ=255)
                                                               tgttgtgttgTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGaa                           <  1:620274/93‑1 (MQ=255)
                                                                 ttgtgttgtTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATc                         >  1:425462/1‑93 (MQ=255)
                                                                    tgttgtTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGc                      <  1:1623435/93‑1 (MQ=255)
                                                                     gttgttGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCt                     >  1:6075524/1‑93 (MQ=255)
                                                                     gttgttGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCt                     >  1:5927054/1‑93 (MQ=255)
                                                                        gttgCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCAt                  >  1:4361492/1‑93 (MQ=255)
                                                                         ttgCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATc                 <  2:6005490/93‑1 (MQ=255)
                                                                         ttgCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATc                 <  2:72853/93‑1 (MQ=255)
                                                                          tgCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCt                <  1:3419286/93‑1 (MQ=255)
                                                                            ccATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCttt              >  1:568166/1‑93 (MQ=255)
                                                                             cATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCtttt             <  1:4622117/93‑1 (MQ=255)
                                                                             cATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCtttt             >  2:1625990/1‑93 (MQ=255)
                                                                              aTGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTa            <  1:1788255/93‑1 (MQ=255)
                                                                              aTGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTa            >  1:5571633/1‑93 (MQ=255)
                                                                              aTGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTa            >  2:3243995/1‑93 (MQ=255)
                                                                               tGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTAt           <  1:6183430/93‑1 (MQ=255)
                                                                               tGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTAt           >  2:1939896/1‑93 (MQ=255)
                                                                               tGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTAt           <  2:2410314/93‑1 (MQ=255)
                                                                               tGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTAt           >  1:4648580/1‑93 (MQ=255)
                                                                                 gTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGc         <  1:2905827/93‑1 (MQ=255)
                                                                                  tATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCa        >  1:3006503/1‑93 (MQ=255)
                                                                                    ttCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGt      >  1:870229/1‑93 (MQ=255)
                                                                                    ttCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGt      <  2:4494763/93‑1 (MQ=255)
                                                                                       tcCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCt   <  2:2898832/93‑1 (MQ=255)
                                                                                        ccGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTg  <  1:4861265/93‑1 (MQ=255)
                                                                                             |                                                                                       
GCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGC‑ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTG  >  NC_000913/4013679‑4013857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGC‑ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑A‑CCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATGA  >  NC_000913/4013683‑4013865
                                                                                            |                                                                                             
CGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑A‑CCctt                                                                                            >  SRR4302533.5158267/1‑91 (MQ=60)
CGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑A‑CCctt                                                                                            <  SRR4302533.856239/94‑4 (MQ=60)
 GTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTA                                                                                            >  SRR4302533.920412/1‑94 (MQ=60)
  TCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTAT                                                                                           >  SRR4302533.2817580/1‑94 (MQ=60)
  TCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTAT                                                                                           <  SRR4302533.1502382/94‑1 (MQ=60)
  TCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTAT                                                                                           <  SRR4302533.289915/94‑1 (MQ=60)
   CCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATA                                                                                          >  SRR4302533.688525/1‑94 (MQ=60)
   CCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATA                                                                                          <  SRR4302533.6166285/94‑1 (MQ=60)
   CCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGGGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATA                                                                                          >  SRR4302533.2003581/1‑94 (MQ=60)
     GGNGNCNGNACGATGGTCGAAGNGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAA                                                                                        <  SRR4302533.4509231/94‑1 (MQ=60)
     GGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAA                                                                                        >  SRR4302533.3409081/1‑94 (MQ=60)
      GGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAA                                                                                       >  SRR4302533.6277442/1‑94 (MQ=60)
       GGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAA                                                                                      <  SRR4302533.4196124/94‑1 (MQ=60)
       GGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGCTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAA                                                                                      >  SRR4302533.5373267/1‑94 (MQ=60)
        GTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAAT                                                                                     <  SRR4302533.5360775/94‑1 (MQ=60)
         TCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATG                                                                                    >  SRR4302533.4434389/1‑94 (MQ=60)
         TCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATG                                                                                    >  SRR4302533.2649020/1‑94 (MQ=60)
          CTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGT                                                                                   >  SRR4302533.969721/1‑94 (MQ=60)
          CTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGT                                                                                   >  SRR4302533.1644966/1‑94 (MQ=60)
           TGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTT                                                                                  <  SRR4302533.4669326/94‑1 (MQ=60)
           TGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTT                                                                                  >  SRR4302533.5524535/1‑94 (MQ=60)
           TGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTT                                                                                  <  SRR4302533.4350484/94‑1 (MQ=60)
           TGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTT                                                                                  <  SRR4302533.6221205/94‑1 (MQ=60)
            GAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTG                                                                                 >  SRR4302533.4907600/1‑94 (MQ=60)
             AACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTCC‑CCTTATAAAAATGTTGC                                                                                <  SRR4302533.2818188/94‑1 (MQ=60)
             AACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGC                                                                                >  SRR4302533.2429730/1‑94 (MQ=60)
             AACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGC                                                                                <  SRR4302533.1241442/94‑1 (MQ=60)
              ACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCG                                                                               >  SRR4302533.5610288/1‑94 (MQ=60)
              ACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCG                                                                               >  SRR4302533.4128225/1‑94 (MQ=60)
              ACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCG                                                                               <  SRR4302533.5790154/94‑1 (MQ=60)
              ACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCG                                                                               <  SRR4302533.2822073/94‑1 (MQ=60)
                GATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCA                                                                             >  SRR4302533.1034889/1‑94 (MQ=60)
                 ATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAA                                                                            >  SRR4302533.3184554/1‑94 (MQ=60)
                  TGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAAT                                                                           <  SRR4302533.4541646/94‑1 (MQ=60)
                  TGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAAT                                                                           <  SRR4302533.2531663/94‑1 (MQ=60)
                     TCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTT                                                                        <  SRR4302533.6211135/94‑1 (MQ=60)
                     TCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTT                                                                        <  SRR4302533.4691819/94‑1 (MQ=60)
                      CGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTA                                                                       <  SRR4302533.1759407/94‑1 (MQ=60)
                      CGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTA                                                                       <  SRR4302533.5047211/94‑1 (MQ=60)
                      CGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTA                                                                       <  SRR4302533.1782120/94‑1 (MQ=60)
                      CGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTA                                                                       >  SRR4302533.3570435/1‑94 (MQ=60)
                        AAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAAC                                                                     >  SRR4302533.2236292/1‑94 (MQ=60)
                         AGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACT                                                                    >  SRR4302533.123520/1‑94 (MQ=60)
                            GAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATA                                                                 >  SRR4302533.1689096/1‑94 (MQ=60)
                            GAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATA                                                                 >  SRR4302533.5099284/1‑94 (MQ=60)
                             AGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAG                                                                <  SRR4302533.3347612/94‑1 (MQ=60)
                             AGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAG                                                                <  SRR4302533.3462660/94‑1 (MQ=60)
                             AGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAG                                                                >  SRR4302533.4981529/1‑94 (MQ=60)
                             AGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAG                                                                <  SRR4302533.806535/94‑1 (MQ=60)
                             AGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAG                                                                >  SRR4302533.3762779/1‑94 (MQ=60)
                              GAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGT                                                               <  SRR4302533.1613249/94‑1 (MQ=60)
                              GAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGT                                                               >  SRR4302533.5142117/1‑94 (MQ=60)
                              GAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGT                                                               >  SRR4302533.2228510/1‑94 (MQ=60)
                               AGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTC                                                              >  SRR4302533.2617645/1‑94 (MQ=60)
                               AGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTC                                                              >  SRR4302533.5557292/1‑94 (MQ=60)
                               AGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTC                                                              <  SRR4302533.2675009/94‑1 (MQ=60)
                               AGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTC                                                              >  SRR4302533.586114/1‑94 (MQ=60)
                                GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCA                                                             <  SRR4302533.1406103/94‑1 (MQ=60)
                                 gcgATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAG                                                            <  SRR4302533.6245999/91‑1 (MQ=60)
                                  cgATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGC                                                           >  SRR4302533.77627/3‑94 (MQ=60)
                                  cgATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGC                                                           >  SRR4302533.6147259/3‑94 (MQ=60)
                                  cgATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGC                                                           >  SRR4302533.2540203/3‑94 (MQ=60)
                                  cgATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGC                                                           <  SRR4302533.3320304/92‑1 (MQ=60)
                                  G‑ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCA                                                          >  SRR4302533.6091995/1‑94 (MQ=60)
                                  G‑ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGGATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCA                                                          >  SRR4302533.2395594/1‑94 (MQ=60)
                                    ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCAT                                                         <  SRR4302533.1443583/94‑1 (MQ=60)
                                     TGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATG                                                        <  SRR4302533.1382269/94‑1 (MQ=60)
                                     TGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATG                                                        <  SRR4302533.4712724/94‑1 (MQ=60)
                                      GCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGC                                                       >  SRR4302533.1919222/1‑94 (MQ=60)
                                      GCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGC                                                       <  SRR4302533.6116851/94‑1 (MQ=60)
                                      GCAGCAGGTGCAAATCCAGATTGTAGTGTTGTTGCCATGTTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGC                                                       <  SRR4302533.6116850/94‑1 (MQ=60)
                                       CAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTGC‑CCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCA                                                      <  SRR4302533.1742284/94‑1 (MQ=60)
                                       CAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCA                                                      >  SRR4302533.1717250/1‑94 (MQ=60)
                                        AGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAA                                                     >  SRR4302533.1846880/1‑94 (MQ=60)
                                           AGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAA                                                  >  SRR4302533.807195/1‑94 (MQ=60)
                                            GGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAA                                                 >  SRR4302533.1919692/1‑94 (MQ=60)
                                             GTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAAT                                                <  SRR4302533.5840191/94‑1 (MQ=60)
                                             GTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAAT                                                <  SRR4302533.5276618/94‑1 (MQ=60)
                                               GCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCA                                              <  SRR4302533.2814995/94‑1 (MQ=60)
                                                CAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACACATCAC                                             >  SRR4302533.2291543/1‑94 (MQ=60)
                                                 AAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACA                                            >  SRR4302533.4893264/1‑94 (MQ=60)
                                                   ATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATT                                          >  SRR4302533.597766/1‑94 (MQ=60)
                                                   ATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATT                                          <  SRR4302533.6112868/94‑1 (MQ=60)
                                                    TCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTG                                         <  SRR4302533.542163/94‑1 (MQ=60)
                                                    TCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTG                                         <  SRR4302533.5879222/94‑1 (MQ=60)
                                                    TCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTG                                         >  SRR4302533.2531379/1‑94 (MQ=60)
                                                    TCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTC                                         >  SRR4302533.3754647/1‑94 (MQ=60)
                                                     CCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGC                                        >  SRR4302533.4985532/1‑94 (MQ=60)
                                                     CCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGC                                        >  SRR4302533.5296101/1‑94 (MQ=60)
                                                       AGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCT                                      <  SRR4302533.3827150/94‑1 (MQ=60)
                                                        GATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTG                                     >  SRR4302533.4560600/1‑94 (MQ=60)
                                                        GATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTG                                     <  SRR4302533.5807964/94‑1 (MQ=60)
                                                         ATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGA                                    >  SRR4302533.4656035/1‑94 (MQ=60)
                                                           TGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAAT                                  <  SRR4302533.623916/94‑1 (MQ=60)
                                                           TGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAAT                                  >  SRR4302533.5827426/1‑94 (MQ=60)
                                                           TGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAAT                                  >  SRR4302533.2959083/1‑94 (MQ=60)
                                                            GTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATC                                 >  SRR4302533.152434/1‑94 (MQ=60)
                                                            GTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATC                                 >  SRR4302533.428000/1‑94 (MQ=60)
                                                             TTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCG                                <  SRR4302533.5945753/94‑1 (MQ=60)
                                                                TGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCT                             <  SRR4302533.912543/94‑1 (MQ=60)
                                                                TGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCT                             >  SRR4302533.6112273/1‑94 (MQ=60)
                                                                TGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCT                             >  SRR4302533.5962885/1‑94 (MQ=60)
                                                                TGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCT                             <  SRR4302533.1633268/94‑1 (MQ=60)
                                                                  TTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCA                           <  SRR4302533.5161443/94‑1 (MQ=60)
                                                                   TGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCAT                          >  SRR4302533.4387819/1‑94 (MQ=60)
                                                                     TTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCT                        <  SRR4302533.5737124/94‑1 (MQ=60)
                                                                     TTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCT                        <  SRR4302533.6202222/94‑1 (MQ=60)
                                                                      TGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTT                       <  SRR4302533.3440413/94‑1 (MQ=60)
                                                                       GCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTT                      >  SRR4302533.571526/1‑94 (MQ=60)
                                                                       GCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCACGCAACAAATCACATTGCCTGAATCGGCTCATCTTT                      <  SRR4302533.1369982/94‑1 (MQ=60)
                                                                         CATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTA                    >  SRR4302533.5605306/1‑94 (MQ=60)
                                                                         CATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTA                    >  SRR4302533.4395191/1‑94 (MQ=60)
                                                                         CATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTA                    <  SRR4302533.4650129/94‑1 (MQ=60)
                                                                          ATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTAT                   >  SRR4302533.441826/1‑94 (MQ=60)
                                                                          ATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTAT                   >  SRR4302533.2539723/1‑94 (MQ=60)
                                                                          ATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTAT                   >  SRR4302533.4676746/1‑94 (MQ=60)
                                                                          ATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTAT                   <  SRR4302533.1799126/94‑1 (MQ=60)
                                                                           TGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATG                  <  SRR4302533.6220556/94‑1 (MQ=60)
                                                                             GTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCA                <  SRR4302533.2923670/94‑1 (MQ=60)
                                                                             GTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCA                >  SRR4302533.3024893/1‑94 (MQ=60)
                                                                              TATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTTCCTGAATCGGCTCATCTTTTATGCAG               <  SRR4302533.3960626/94‑1 (MQ=60)
                                                                              TATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAG               <  SRR4302533.4906558/94‑1 (MQ=60)
                                                                              TATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAG               <  SRR4302533.4937953/94‑1 (MQ=60)
                                                                               ATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGT              >  SRR4302533.875337/1‑94 (MQ=60)
                                                                               ATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGT              >  SRR4302533.3571787/1‑94 (MQ=60)
                                                                                  CTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCT           >  SRR4302533.4751081/1‑94 (MQ=60)
                                                                                  CTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCT           <  SRR4302533.927179/94‑1 (MQ=60)
                                                                                  CTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCT           >  SRR4302533.804044/1‑94 (MQ=60)
                                                                                    CCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGC         >  SRR4302533.1603799/1‑94 (MQ=60)
                                                                                    CCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGC         <  SRR4302533.4890728/94‑1 (MQ=60)
                                                                                    CCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGC         <  SRR4302533.706808/94‑1 (MQ=60)
                                                                                      GT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATTACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAG       >  SRR4302533.2676979/1‑94 (MQ=60)
                                                                                        tacCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGA      >  SRR4302533.4816739/4‑94 (MQ=60)
                                                                                        tacCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGA      >  SRR4302533.4327119/4‑94 (MQ=60)
                                                                                         acCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAA     >  SRR4302533.3899114/3‑94 (MQ=60)
                                                                                         acCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAA     >  SRR4302533.2065201/3‑94 (MQ=60)
                                                                                         C‑CCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAAT    <  SRR4302533.901486/94‑1 (MQ=60)
                                                                                         C‑CCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAAT    >  SRR4302533.4235477/1‑94 (MQ=60)
                                                                                           CCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATG   <  SRR4302533.5106154/94‑1 (MQ=60)
                                                                                           CCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATG   >  SRR4302533.2952390/1‑94 (MQ=60)
                                                                                            CTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATGA  >  SRR4302533.642745/1‑94 (MQ=60)
                                                                                            CTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATGA  >  SRR4302533.2350337/1‑94 (MQ=60)
                                                                                            CTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATGA  >  SRR4302533.6341203/1‑94 (MQ=60)
                                                                                            |                                                                                             
CGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGC‑ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑A‑CCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATGA  >  NC_000913/4013683‑4013865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑