Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,016,743	C→T	P101L (CCC→CTC)	ubiE →	UbiE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,016,743	0	C	T	100.0%	915.1 / NA	245	P101L (CCC→CTC)	ubiE	UbiE
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (107/138); total (107/138)

Only 100 of 247 total aligned reads displayed.

CTGGTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCCCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCG  >  NC_000913/4016653‑4016831

ctggTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCa                                                                                        <  2:517080/93‑1 (MQ=255)

ctggTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCa                                                                                        >  1:1909189/1‑93 (MQ=255)

 tggtggCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCaa                                                                                       >  1:4900063/1‑93 (MQ=255)

  ggtggCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATGCATGCTCaaa                                                                                      >  1:150667/1‑93 (MQ=255)

    tggCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAAt                                                                                    <  2:5764856/93‑1 (MQ=255)

     ggCCCCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATg                                                                                   <  1:1113113/93‑1 (MQ=255)

     ggCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATg                                                                                   <  1:3381471/93‑1 (MQ=255)

      gCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATgg                                                                                  >  1:4108871/1‑93 (MQ=255)

        cccGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGc                                                                                <  2:3176066/92‑1 (MQ=255)

        aCCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGc                                                                                <  2:1391350/93‑1 (MQ=255)

        aCCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGc                                                                                >  1:2816543/1‑93 (MQ=255)

         ccGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGcc                                                                               <  1:1104656/93‑1 (MQ=255)

         ccGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGcc                                                                               >  2:792534/1‑93 (MQ=255)

         ccGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGcc                                                                               <  2:5430517/93‑1 (MQ=255)

         ccGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGcc                                                                               <  1:1290877/93‑1 (MQ=255)

          cGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCcg                                                                              >  1:2721240/1‑93 (MQ=255)

           ggCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCcgc                                                                             <  1:1352259/93‑1 (MQ=255)

             cGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCga                                                                           <  2:936911/93‑1 (MQ=255)

             cGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCga                                                                           <  1:3671974/93‑1 (MQ=255)

              gACCTGACAGCGAAATTCTCCCGCCTGGTCAGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCgag                                                                          >  2:4765054/1‑93 (MQ=255)

               aCCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCgaga                                                                         >  1:5951655/1‑93 (MQ=255)

                ccTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGaa                                                                        <  2:2337253/93‑1 (MQ=255)

                ccTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGaa                                                                        >  2:3627542/1‑93 (MQ=255)

                ccTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGaa                                                                        >  2:2481495/1‑93 (MQ=255)

                 cTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAg                                                                       >  2:6133567/1‑93 (MQ=255)

                 cTGACAGCGAAATTCGCCCGCCTGGTCGGAGAAACTGGCAAAGTCGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAg                                                                       <  1:356609/93‑1 (MQ=255)

                 cTGACAGCGAAAGTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAg                                                                       <  1:5382426/93‑1 (MQ=255)

                  tGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGc                                                                      <  1:330828/93‑1 (MQ=255)

                  tGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGc                                                                      >  2:3965497/1‑93 (MQ=255)

                  tGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGc                                                                      <  1:3993454/93‑1 (MQ=255)

                         gAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTaa                                                               <  2:6309016/93‑1 (MQ=255)

                          aaaTTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAat                                                              <  1:297134/93‑1 (MQ=255)

                           aaTTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAata                                                             <  2:4095720/93‑1 (MQ=255)

                           aaTTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAata                                                             >  2:4287446/1‑93 (MQ=255)

                             ttCTCCCGCCTGGTCGGAGAAACTGGCACAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATc                                                           <  1:6061643/93‑1 (MQ=255)

                             ttCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATc                                                           <  1:1856749/93‑1 (MQ=255)

                             ttCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATc                                                           <  2:3859333/93‑1 (MQ=255)

                               ctcCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCgg                                                         <  1:4172293/93‑1 (MQ=255)

                                tcCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGgt                                                        <  1:1626293/93‑1 (MQ=255)

                                 cccGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGgtg                                                       >  2:304837/1‑93 (MQ=255)

                                 cccGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGgtg                                                       >  1:80518/1‑93 (MQ=255)

                                  ccGCCTGGTCGAAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCAGCGTAATTTCGgtgg                                                      <  2:4994531/93‑2 (MQ=255)

                                   cGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAACGGGCCGCGAGAAGCTGCGTAATATCGGtgtg                                                     >  1:3016236/1‑93 (MQ=255)

                                    gCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGa                                                    <  2:548481/93‑1 (MQ=255)

                                      cTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGAtt                                                  <  1:6101501/93‑1 (MQ=255)

                                       tGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTg                                                 >  2:2435833/1‑93 (MQ=255)

                                       tGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTg                                                 >  2:3225615/1‑93 (MQ=255)

                                       tGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTg                                                 >  1:1225826/1‑93 (MQ=255)

                                       tGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTg                                                 <  2:5547556/93‑1 (MQ=255)

                                         gTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGc                                               <  1:2612733/93‑1 (MQ=255)

                                         gTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGc                                               >  2:1999229/1‑93 (MQ=255)

                                           cGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCaa                                             <  1:656766/93‑1 (MQ=255)

                                           cGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCaa                                             <  2:1695340/93‑1 (MQ=255)

                                           cGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCaa                                             <  1:5461317/93‑1 (MQ=255)

                                            ggAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAAc                                            >  2:2754262/1‑93 (MQ=255)

                                            ggAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAAc                                            >  2:1472867/1‑93 (MQ=255)

                                            ggAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAAc                                            <  1:6266406/93‑1 (MQ=255)

                                             gagaAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACg                                           >  2:3160293/1‑93 (MQ=255)

                                               gaAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGtt                                         >  2:600238/1‑93 (MQ=255)

                                                aaaCTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTg                                        >  2:3101238/1‑93 (MQ=255)

                                                 aaCTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGa                                       <  1:2081560/93‑1 (MQ=255)

                                                  aCTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAg                                      <  1:6178665/93‑1 (MQ=255)

                                                  aCTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAg                                      >  2:2898371/1‑93 (MQ=255)

                                                   cTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGt                                     >  1:3859211/1‑93 (MQ=255)

                                                    tGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTa                                    <  1:4531643/93‑1 (MQ=255)

                                                      gCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATg                                  >  2:2223220/1‑93 (MQ=255)

                                                       cAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGt                                 <  2:945206/93‑1 (MQ=255)

                                                       cAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGt                                 <  1:3141931/93‑1 (MQ=255)

                                                        aaaGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGtt                                <  1:6002061/93‑1 (MQ=255)

                                                         aaGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTc                               >  2:1605742/1‑93 (MQ=255)

                                                         aaGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTc                               <  2:1281476/93‑1 (MQ=255)

                                                          aGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCa                              <  2:2499679/93‑1 (MQ=255)

                                                          aGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCa                              <  2:1811494/93‑1 (MQ=255)

                                                            tGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAgg                            >  1:83991/1‑93 (MQ=255)

                                                             ggTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGc                           <  1:567621/93‑1 (MQ=255)

                                                                ccTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGaa                        <  2:2598596/93‑1 (MQ=255)

                                                                ccTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGaa                        >  1:2262682/1‑93 (MQ=255)

                                                                ccTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGaa                        >  2:5213391/1‑93 (MQ=255)

                                                                ccTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGaa                        <  2:180041/93‑1 (MQ=255)

                                                                 cTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCTAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAAc                       <  2:5635603/93‑1 (MQ=255)

                                                                 cTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAAc                       >  2:4191567/1‑93 (MQ=255)

                                                                  ttGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACg                      >  1:4677280/1‑93 (MQ=255)

                                                                  ttGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACg                      >  1:5041662/1‑93 (MQ=255)

                                                                    gCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCt                    <  1:2225618/93‑1 (MQ=255)

                                                                     cTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTg                   <  2:3551626/93‑1 (MQ=255)

                                                                      tGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGa                  <  2:4599461/93‑1 (MQ=255)

                                                                      tGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGa                  >  1:2426627/1‑93 (MQ=255)

                                                                      tGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGa                  <  1:5152514/93‑1 (MQ=255)

                                                                      tGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGa                  >  2:4363418/1‑93 (MQ=255)

                                                                      ctatatCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGa                  <  2:2835011/91‑1 (MQ=255)

                                                                       gATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAg                 <  1:1030326/93‑1 (MQ=255)

                                                                          atCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGgcg              >  2:4967407/1‑93 (MQ=255)

                                                                            cAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATCGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCt            <  2:5080313/93‑1 (MQ=255)

                                                                             aaTGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTg           >  1:3704762/1‑93 (MQ=255)

                                                                                gAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCg        >  1:177718/1‑93 (MQ=255)

                                                                                gAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCg        >  2:5895857/1‑93 (MQ=255)

                                                                                   tCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTc     <  2:6039407/93‑1 (MQ=255)

                                                                                   tCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTc     >  1:2526887/1‑93 (MQ=255)

                                                                                    ccATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTcc    >  2:5288618/1‑93 (MQ=255)

                                                                                    ccATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTcc    >  1:1655561/1‑93 (MQ=255)

CTGGTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCCCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCG  >  NC_000913/4016653‑4016831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGGCTGGTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCCCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAA  >  NC_000913/4016650‑4016836

TGTCTGGTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCT                                                                                               <  SRR4302533.426960/94‑1 (MQ=60)

TGGCTGGTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCT                                                                                               >  SRR4302533.4072321/1‑94 (MQ=60)

TGGCTGGTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCT                                                                                               >  SRR4302533.4072320/1‑94 (MQ=60)

 GGCTGGTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTC                                                                                              <  SRR4302533.117674/94‑1 (MQ=60)

  GCTGGTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCA                                                                                             >  SRR4302533.5532135/1‑94 (MQ=60)

  GCTGGTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCA                                                                                             >  SRR4302533.1920835/1‑94 (MQ=60)

  GCTGGTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCA                                                                                             <  SRR4302533.1644149/94‑1 (MQ=60)

   CTGGTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAA                                                                                            >  SRR4302533.4929754/1‑94 (MQ=60)

   CTGGTGGCACAGGCGACCTGACAGCAAAATTCTCCCGCCTGGTCGGAGAAACTGGAAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAA                                                                                            <  SRR4302533.6066742/94‑1 (MQ=60)

    TGGTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATGCATGCTCAAA                                                                                           >  SRR4302533.151633/1‑94 (MQ=60)

       TGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATG                                                                                        <  SRR4302533.5268235/94‑1 (MQ=60)

        GGCCCCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGG                                                                                       <  SRR4302533.1119715/94‑1 (MQ=60)

        GGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGG                                                                                       <  SRR4302533.3402344/94‑1 (MQ=60)

        GGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGG                                                                                       >  SRR4302533.4133853/1‑94 (MQ=60)

         GCACCGGCGACCTGACAGCGAAATGCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGG                                                                                      <  SRR4302533.6202883/94‑1 (MQ=60)

          CACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGC                                                                                     >  SRR4302533.2833882/1‑94 (MQ=60)

           ACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCC                                                                                    <  SRR4302533.1355152/94‑1 (MQ=60)

           ACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCC                                                                                    >  SRR4302533.4010422/1‑94 (MQ=60)

           ACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCC                                                                                    <  SRR4302533.4145653/94‑1 (MQ=60)

            CGGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCG                                                                                   <  SRR4302533.1227689/94‑1 (MQ=60)

            CCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCG                                                                                   <  SRR4302533.1298652/94‑1 (MQ=60)

            CCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCG                                                                                   >  SRR4302533.2737942/1‑94 (MQ=60)

            CCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCG                                                                                   <  SRR4302533.4068345/94‑1 (MQ=60)

            CCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCG                                                                                   <  SRR4302533.1111200/94‑1 (MQ=60)

            CCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCG                                                                                   <  SRR4302533.505802/94‑1 (MQ=60)

              GGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCG                                                                                 <  SRR4302533.1360392/94‑1 (MQ=60)

              GGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCG                                                                                 <  SRR4302533.6023936/94‑1 (MQ=60)

                CGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAG                                                                               <  SRR4302533.3694542/94‑1 (MQ=60)

                 GACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGA                                                                              >  SRR4302533.639360/1‑94 (MQ=60)

                 GACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGA                                                                              >  SRR4302533.5987656/1‑94 (MQ=60)

                   CCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAG                                                                            >  SRR4302533.4980507/1‑94 (MQ=60)

                    CTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGC                                                                           >  SRR4302533.4530313/1‑94 (MQ=60)

                    CTGACAGCGAAATTCGCCCGCCTGGTCGGAGAAACTGGCAAAGTCGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGC                                                                           <  SRR4302533.358782/94‑1 (MQ=60)

                    CTGACAGCGAAAGTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGC                                                                           <  SRR4302533.5414764/94‑1 (MQ=60)

                     TGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCT                                                                          <  SRR4302533.3609818/94‑1 (MQ=60)

                     TGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCT                                                                          <  SRR4302533.332850/94‑1 (MQ=60)

                     TGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCT                                                                          <  SRR4302533.6112630/94‑1 (MQ=60)

                     TGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCT                                                                          <  SRR4302533.4017767/94‑1 (MQ=60)

                      GACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTG                                                                         <  SRR4302533.3348882/94‑1 (MQ=60)

                      GACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTG                                                                         <  SRR4302533.612949/94‑1 (MQ=60)

                        CAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCG                                                                       <  SRR4302533.314523/94‑1 (MQ=60)

                        CAGCGAAATCCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCG                                                                       <  SRR4302533.2627327/94‑1 (MQ=60)

                         AGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGT                                                                      <  SRR4302533.6036820/94‑1 (MQ=60)

                            GAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAAT                                                                   >  SRR4302533.2109669/1‑94 (MQ=60)

                            GAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAAT                                                                   >  SRR4302533.1297867/1‑94 (MQ=60)

                             AAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATA                                                                  <  SRR4302533.298954/94‑1 (MQ=60)

                                TTCTCCCGCCTGGTCGGAGAAACTGGCACAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCG                                                               <  SRR4302533.6098338/94‑1 (MQ=60)

                                TTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCG                                                               <  SRR4302533.1868076/94‑1 (MQ=60)

                                  CTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGT                                                             <  SRR4302533.4197575/94‑1 (MQ=60)

                                   TCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTG                                                            <  SRR4302533.1636142/94‑1 (MQ=60)

                                   TCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTG                                                            >  SRR4302533.2467470/1‑94 (MQ=60)

                                   TCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTG                                                            <  SRR4302533.1752642/94‑1 (MQ=60)

                                   TCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTG                                                            <  SRR4302533.5164268/94‑1 (MQ=60)

                                   TCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTG                                                            >  SRR4302533.81031/1‑94 (MQ=60)

                                     CCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAACGGGCCGCGAGAAGCTGCGTAATATCGGTGTG                                                          >  SRR4302533.3034684/1‑94 (MQ=60)

                                         CTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTG                                                      >  SRR4302533.1233170/1‑94 (MQ=60)

                                         CTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTG                                                      <  SRR4302533.6138341/94‑1 (MQ=60)

                                         CTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTG                                                      >  SRR4302533.4724672/1‑94 (MQ=60)

                                         CTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTG                                                      <  SRR4302533.4651758/94‑1 (MQ=60)

                                            GTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCA                                                   <  SRR4302533.300760/94‑1 (MQ=60)

                                            GTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCA                                                   <  SRR4302533.2628770/94‑1 (MQ=60)

                                              CGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAAC                                                 <  SRR4302533.5494219/94‑1 (MQ=60)

                                              CGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAAC                                                 <  SRR4302533.5616456/94‑1 (MQ=60)

                                              CGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAAC                                                 <  SRR4302533.660641/94‑1 (MQ=60)

                                              CGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAAC                                                 >  SRR4302533.6246377/1‑94 (MQ=60)

                                              CGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAAC                                                 >  SRR4302533.2184558/1‑94 (MQ=60)

                                               GGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACG                                                <  SRR4302533.6303849/94‑1 (MQ=60)

                                               GGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACG                                                >  SRR4302533.3721282/1‑94 (MQ=60)

                                               GGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACG                                                >  SRR4302533.1691613/1‑94 (MQ=60)

                                               GGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACG                                                >  SRR4302533.1340973/1‑94 (MQ=60)

                                                 AGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTT                                              <  SRR4302533.1588748/94‑1 (MQ=60)

                                                    AACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAG                                           <  SRR4302533.2094370/94‑1 (MQ=60)

                                                     ACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGT                                          <  SRR4302533.6215776/94‑1 (MQ=60)

                                                     ACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGT                                          >  SRR4302533.3882773/1‑94 (MQ=60)

                                                     ACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGT                                          >  SRR4302533.3175460/1‑94 (MQ=60)

                                                      CTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTA                                         <  SRR4302533.1916410/94‑1 (MQ=60)

                                                       TGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTAT                                        <  SRR4302533.4559083/94‑1 (MQ=60)

                                                          CAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTT                                     <  SRR4302533.3161246/94‑1 (MQ=60)

                                                          CAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTT                                     <  SRR4302533.5271546/94‑1 (MQ=60)

                                                           AAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTC                                    <  SRR4302533.6038423/94‑1 (MQ=60)

                                                             AGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAG                                  <  SRR4302533.546676/94‑1 (MQ=60)

                                                              GTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGG                                 >  SRR4302533.84525/1‑94 (MQ=60)

                                                               TGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGC                                <  SRR4302533.1653203/94‑1 (MQ=60)

                                                                GGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCG                               >  SRR4302533.696642/1‑94 (MQ=60)

                                                                GGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCG                               <  SRR4302533.2413447/94‑1 (MQ=60)

                                                                GGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCG                               <  SRR4302533.2246751/94‑1 (MQ=60)

                                                                GGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCG                               <  SRR4302533.2088655/94‑1 (MQ=60)

                                                                GGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCG                               <  SRR4302533.570981/94‑1 (MQ=60)

                                                                GGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCG                               <  SRR4302533.1855984/94‑1 (MQ=60)

                                                                  TCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAA                             >  SRR4302533.2276677/1‑94 (MQ=60)

                                                                   CCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAAC                            >  SRR4302533.3755933/1‑94 (MQ=60)

                                                                    CTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACG                           >  SRR4302533.4705626/1‑94 (MQ=60)

                                                                    CTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACG                           >  SRR4302533.5072175/1‑94 (MQ=60)

                                                                    CTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACG                           <  SRR4302533.3090679/94‑1 (MQ=60)

                                                                     TTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGC                          <  SRR4302533.4068431/94‑1 (MQ=60)

                                                                      TGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCT                         >  SRR4302533.5864104/1‑94 (MQ=60)

                                                                       GCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTG                        >  SRR4302533.387561/1‑94 (MQ=60)

                                                                       GCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTG                        <  SRR4302533.2239357/94‑1 (MQ=60)

                                                                        CTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGA                       <  SRR4302533.3647491/94‑1 (MQ=60)

                                                                        CTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGA                       >  SRR4302533.1580123/1‑94 (MQ=60)

                                                                        CTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGA                       >  SRR4302533.2441631/1‑94 (MQ=60)

                                                                        CTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGA                       >  SRR4302533.2705323/1‑94 (MQ=60)

                                                                         TGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAG                      <  SRR4302533.3540844/94‑1 (MQ=60)

                                                                         TGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAG                      <  SRR4302533.2956853/94‑1 (MQ=60)

                                                                         TGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAG                      >  SRR4302533.1892472/1‑94 (MQ=60)

                                                                         TGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAG                      <  SRR4302533.5183638/94‑1 (MQ=60)

                                                                          GATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGG                     <  SRR4302533.1036385/94‑1 (MQ=60)

                                                                           ATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGC                    <  SRR4302533.111422/94‑1 (MQ=60)

                                                                           ATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGC                    >  SRR4302533.6216293/1‑94 (MQ=60)

                                                                            TATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCG                   <  SRR4302533.737097/94‑1 (MQ=60)

                                                                             ATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGCTCAGGCGAACGCTGAGGCGC                  >  SRR4302533.828097/1‑94 (MQ=60)

                                                                              TGAATGAATCCATGCTCAAAAGGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCT                 <  SRR4302533.4544150/94‑1 (MQ=60)

                                                                               CAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTG                >  SRR4302533.3727515/1‑94 (MQ=60)

                                                                               CAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTG                <  SRR4302533.3399760/94‑1 (MQ=60)

                                                                               CAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTG                <  SRR4302533.1191145/94‑1 (MQ=60)

                                                                                 ATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCC              <  SRR4302533.2829286/94‑1 (MQ=60)

                                                                                 ATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCC              >  SRR4302533.2325076/1‑94 (MQ=60)

                                                                                 ATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCC              <  SRR4302533.1083077/94‑1 (MQ=60)

                                                                                  TGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCG             >  SRR4302533.178831/1‑94 (MQ=60)

                                                                                   GAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGT            >  SRR4302533.2735935/1‑94 (MQ=60)

                                                                                   GAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGT            <  SRR4302533.2881832/94‑1 (MQ=60)

                                                                                     ATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTC          >  SRR4302533.2542443/1‑94 (MQ=60)

                                                                                      TCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCC         >  SRR4302533.1665583/1‑94 (MQ=60)

                                                                                        CATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCG       >  SRR4302533.1502315/1‑94 (MQ=60)

                                                                                           GCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATTTTCAGCCGAACGCTGAGGCGTTGCCGTTCCCGGAT    >  SRR4302533.348998/1‑94 (MQ=60)

                                                                                           GCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGAT    <  SRR4302533.2885319/94‑1 (MQ=60)

                                                                                            CTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATA   <  SRR4302533.5937411/94‑1 (MQ=60)

                                                                                             TCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAA  <  SRR4302533.4631100/94‑1 (MQ=60)

TGGCTGGTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCCCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAA  >  NC_000913/4016650‑4016836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`