Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,017,557	A→G	E116G (GAG→GGG)	yigP →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,017,557	0	A	G	100.0%	777.8 / NA	244	E116G (GAG→GGG)	yigP	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (99/145); total (99/145)

Only 100 of 250 total aligned reads displayed.

AGCTTACCGCACTGATTCGCAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGAGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAGGCGCAAAGTTCC  >  NC_000913/4017467‑4017646

agcTTACCGCACTGATTCGCAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGt                                                                                         <  2:4979523/93‑1 (MQ=255)

  cTTACCGCACTGATTCGCAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTc                                                                                       >  1:4981818/1‑93 (MQ=255)

   ttACCGCACTGATTCGCAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCg                                                                                      >  2:4429398/1‑93 (MQ=255)

   ttACCGCACTGATTCGCAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCg                                                                                      >  2:1245858/1‑93 (MQ=255)

    tACCGCACTGATTCGCAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGa                                                                                     >  1:3891853/1‑93 (MQ=255)

    tACCGCACTGATTCGCAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGa                                                                                     >  2:5586057/1‑93 (MQ=255)

    tACCGCACTGATTCGCAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGa                                                                                     >  1:75894/1‑93 (MQ=255)

       cGCACTGATTCGCAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGAccc                                                                                  >  2:2924477/1‑93 (MQ=255)

       cGCACTGATTCGCAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGAccc                                                                                  <  2:1823840/93‑1 (MQ=255)

        gCACTGATTCGCAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCt                                                                                 >  1:5743511/1‑93 (MQ=255)

        gCACTGATTCGCAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCt                                                                                 <  2:581942/93‑1 (MQ=255)

        gCACTGATTCGCAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCt                                                                                 <  2:4056361/93‑1 (MQ=255)

          aCTGATTCGCAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGc                                                                               <  2:2480300/93‑1 (MQ=255)

           cTGATTCGCAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCg                                                                              >  2:2747058/1‑93 (MQ=255)

            tGATTCGCAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCgg                                                                             <  1:268637/93‑1 (MQ=255)

              aTTCGCAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGaa                                                                           <  2:3761303/93‑1 (MQ=255)

               ttCGCAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAAc                                                                          <  2:1509848/93‑1 (MQ=255)

               ttCGCAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAAc                                                                          <  1:1001450/93‑1 (MQ=255)

               ttCGCAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAAc                                                                          <  1:3308736/93‑1 (MQ=255)

                   cAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAActgct                                                                      <  1:5360695/93‑1 (MQ=255)

                   cAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAActgct                                                                      <  2:3969773/93‑1 (MQ=255)

                   cAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAActgct                                                                      <  2:4883044/93‑1 (MQ=255)

                    aGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAActgctg                                                                     >  1:2962287/1‑93 (MQ=255)

                    aGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAActgctg                                                                     >  2:1927339/1‑93 (MQ=255)

                      tggtgAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGc                                                                   >  2:3099580/1‑93 (MQ=255)

                         tgAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGcccc                                                                <  1:2204774/93‑1 (MQ=255)

                         tgAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGcccc                                                                <  1:1829250/93‑1 (MQ=255)

                         tgAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGcccc                                                                <  2:5025085/93‑1 (MQ=255)

                         tgAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGcccc                                                                >  1:1514826/1‑93 (MQ=255)

                           aGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCtt                                                              >  2:1203702/1‑93 (MQ=255)

                            gCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTta                                                             <  1:5227457/93‑1 (MQ=255)

                             ctcgAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGAAGGGTTCGACCCTGCGGAACTGCTGGCCCCTtat                                                            >  1:1647795/4‑93 (MQ=255)

                             cTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTtat                                                            <  1:2375669/93‑1 (MQ=255)

                             cTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTtat                                                            <  2:531338/93‑1 (MQ=255)

                              tGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTtata                                                           >  2:1647561/1‑93 (MQ=255)

                              tGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTtata                                                           <  1:5105742/93‑1 (MQ=255)

                              tGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTtata                                                           >  1:3255832/1‑93 (MQ=255)

                                gAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATAcc                                                         <  2:5228058/93‑1 (MQ=255)

                                gAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATAcc                                                         <  1:1104388/93‑1 (MQ=255)

                                gAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATAcc                                                         >  1:2062275/1‑93 (MQ=255)

                                     gCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGTCCCCTTATACCGGTGa                                                    <  2:75963/93‑1 (MQ=255)

                                     gCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGa                                                    <  2:294644/93‑1 (MQ=255)

                                       aGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGata                                                  <  2:4490199/93‑1 (MQ=255)

                                        gggCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGatat                                                 <  2:525409/93‑1 (MQ=255)

                                        gggCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGatat                                                 >  2:4741757/1‑93 (MQ=255)

                                        gggCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGatat                                                 <  1:2613851/93‑1 (MQ=255)

                                         ggCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATc                                                <  2:68293/93‑1 (MQ=255)

                                         ggCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATc                                                >  1:6111145/1‑93 (MQ=255)

                                             atatTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGCTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATcgccg                                            >  2:5687903/1‑93 (MQ=255)

                                              tatTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATcgccgc                                           >  1:81425/1‑93 (MQ=255)

                                               atTCAGGCGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATAACGGTGATATCGCCGCt                                          >  2:174012/1‑93 (MQ=255)

                                                ttCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTg                                         >  2:787506/1‑93 (MQ=255)

                                                ttCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTg                                         >  1:1759825/1‑93 (MQ=255)

                                                   aGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAg                                      <  2:1093689/93‑1 (MQ=255)

                                                    ggtggtGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAgg                                     <  2:4326159/93‑1 (MQ=255)

                                                    ggtggtGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAgg                                     <  2:2870260/93‑1 (MQ=255)

                                                      tggtgCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGaa                                   <  2:2373456/93‑1 (MQ=255)

                                                       ggtgCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAAt                                  <  1:4850343/93‑1 (MQ=255)

                                                       ggtgCAAAACGTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCTGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAAt                                  <  1:3074177/93‑1 (MQ=255)

                                                        gtgCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATc                                 <  1:4786731/93‑1 (MQ=255)

                                                         tgCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCa                                <  2:1421818/93‑1 (MQ=255)

                                                          gCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAg                               >  1:1794594/1‑93 (MQ=255)

                                                          gCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAg                               <  2:548461/93‑1 (MQ=255)

                                                          gCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAg                               <  1:4561191/93‑1 (MQ=255)

                                                          gCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAg                               >  1:1726189/1‑93 (MQ=255)

                                                            aaaaCTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCa                             <  2:4185377/93‑1 (MQ=255)

                                                            aaaaCTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCa                             >  2:3600934/1‑93 (MQ=255)

                                                              aaCTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCaaa                           >  1:4144541/1‑93 (MQ=255)

                                                               aCTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAg                          <  1:3594355/93‑1 (MQ=255)

                                                                 ttCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGcc                        >  2:2895747/1‑93 (MQ=255)

                                                                  tCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGTCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCa                       <  1:2013127/93‑1 (MQ=255)

                                                                  tCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCa                       >  2:4003115/1‑93 (MQ=255)

                                                                   cGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCAt                      <  2:2114193/93‑1 (MQ=255)

                                                                    gTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATg                     <  1:853706/93‑1 (MQ=255)

                                                                    gTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATg                     <  1:5441799/93‑1 (MQ=255)

                                                                    gTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATg                     >  2:2214329/1‑93 (MQ=255)

                                                                    gTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATg                     <  1:1607473/93‑1 (MQ=255)

                                                                    gTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATg                     <  1:5863992/93‑1 (MQ=255)

                                                                     ttGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATgc                    >  1:1923548/1‑93 (MQ=255)

                                                                      tGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATgcg                   <  2:1680203/93‑1 (MQ=255)

                                                                        cgcTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGcgcg                 <  1:2867376/93‑1 (MQ=255)

                                                                        cgcTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGcgcg                 <  1:4328328/93‑1 (MQ=255)

                                                                         gcTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCCTGCGCgg                >  2:596458/1‑93 (MQ=255)

                                                                         gcTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCgg                >  2:4874664/1‑93 (MQ=255)

                                                                         gcTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCgg                >  1:3537974/1‑93 (MQ=255)

                                                                           tGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAg              <  2:6268309/93‑1 (MQ=255)

                                                                            ggCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAgg             >  1:1381288/1‑93 (MQ=255)

                                                                            cgCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAgg             <  1:5313805/92‑1 (MQ=255)

                                                                             gCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAGgc            <  1:5907648/93‑1 (MQ=255)

                                                                              cAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAGgcg           >  2:4362079/1‑93 (MQ=255)

                                                                              cAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAGgcg           >  2:3294131/1‑93 (MQ=255)

                                                                                 aTCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATCTCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAGGCGCaa        <  1:4432831/93‑1 (MQ=255)

                                                                                  tCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAGGCGCaaa       <  2:4724016/93‑1 (MQ=255)

                                                                                  tCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAGGCGCaaa       >  2:1004708/1‑93 (MQ=255)

                                                                                  tCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAGGCGCaaa       >  1:4064859/1‑93 (MQ=255)

                                                                                   cTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAGGCGCAAAg      >  1:2523735/1‑93 (MQ=255)

                                                                                   cTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAGGCGCAAAg      <  2:2579524/93‑1 (MQ=255)

                                                                                   cTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAGGCGCAAAg      <  2:1615166/93‑1 (MQ=255)

                                                                                       cAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAGGCGCAAAGTTcc  <  1:3132381/93‑1 (MQ=255)

                                                                                       cAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAGGCGCAAAGTTcc  <  1:5028643/93‑1 (MQ=255)

AGCTTACCGCACTGATTCGCAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGAGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAGGCGCAAAGTTCC  >  NC_000913/4017467‑4017646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AGCAGCTTACCGCACTGATTCGCAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGAGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAGGCGCAAAGTTCCTGC  >  NC_000913/4017464‑4017649

AGCAGCTTACCGCACTGATTCGCAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGG                                                                                              <  SRR4302533.4742212/94‑1 (MQ=60)

  CAGCTTACCGCACTGATTCGCAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGT                                                                                            <  SRR4302533.3301790/94‑1 (MQ=60)

  CAGCTTACCGCACTGATTCGCAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGT                                                                                            <  SRR4302533.2703813/94‑1 (MQ=60)

  CAGCTTACCGCACTGATTCGCAGTGGTGAGCTGGAAGTGCAGAGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTAGCAGGGT                                                                                            >  SRR4302533.3592379/1‑94 (MQ=60)

   AGCTTACCGCACTGATTCGCAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTT                                                                                           <  SRR4302533.6120418/94‑1 (MQ=60)

    GCTTACCGCACTGATTCGCAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTC                                                                                          >  SRR4302533.5012021/1‑94 (MQ=60)

      TTACCGCACTGATTCGCAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGA                                                                                        >  SRR4302533.76386/1‑94 (MQ=60)

      TTACCGCACTGATTCGCAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGA                                                                                        >  SRR4302533.3915601/1‑94 (MQ=60)

       TACCGCACTGATTCGCAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGAC                                                                                       <  SRR4302533.4358669/94‑1 (MQ=60)

        ACCGCACTGATTCGCAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACC                                                                                      <  SRR4302533.2603950/94‑1 (MQ=60)

        ACCGCACTGATTCGCAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACC                                                                                      >  SRR4302533.1772543/1‑94 (MQ=60)

        ACCGCACTGATTCGCAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACC                                                                                      <  SRR4302533.2614212/94‑1 (MQ=60)

          CGCACTGATTCGCAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCT                                                                                    <  SRR4302533.3111458/94‑1 (MQ=60)

          CGCACTGATTCGCAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCT                                                                                    <  SRR4302533.6146643/94‑1 (MQ=60)

          CGCACTGATTCGCAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCT                                                                                    >  SRR4302533.5778171/1‑94 (MQ=60)

            CACTGATTCGCAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGC                                                                                  >  SRR4302533.3690992/1‑94 (MQ=60)

              CTGATTCGCAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGG                                                                                >  SRR4302533.3252131/1‑94 (MQ=60)

              CTGATTCGCAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGG                                                                                >  SRR4302533.5224363/1‑94 (MQ=60)

               TGATTCGCAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGA                                                                               <  SRR4302533.270267/94‑1 (MQ=60)

                 ATTCGCAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAAC                                                                             <  SRR4302533.3958949/94‑1 (MQ=60)

                  TTCGCAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACT                                                                            <  SRR4302533.1007297/94‑1 (MQ=60)

                  TTCGCAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACT                                                                            <  SRR4302533.3329165/94‑1 (MQ=60)

                    CGCAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGC                                                                          <  SRR4302533.1592766/94‑1 (MQ=60)

                     GCAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCT                                                                         <  SRR4302533.4840073/94‑1 (MQ=60)

                     GCAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCT                                                                         >  SRR4302533.3738892/1‑94 (MQ=60)

                     GCAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCT                                                                         <  SRR4302533.1143532/94‑1 (MQ=60)

                      CAGTGGTGAGCTGGAAGTGCAGGGCTATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTG                                                                        >  SRR4302533.5440601/1‑94 (MQ=60)

                      CAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTG                                                                        <  SRR4302533.5392891/94‑1 (MQ=60)

                      CAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTG                                                                        >  SRR4302533.2980397/1‑94 (MQ=60)

                      CAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTG                                                                        <  SRR4302533.4888100/94‑1 (MQ=60)

                      CAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTG                                                                        >  SRR4302533.573659/1‑94 (MQ=60)

                          GGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCC                                                                    <  SRR4302533.5338328/94‑1 (MQ=60)

                           GTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCC                                                                   >  SRR4302533.5292935/1‑94 (MQ=60)

                           GTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCC                                                                   >  SRR4302533.5390408/1‑94 (MQ=60)

                           GTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCC                                                                   >  SRR4302533.1523950/1‑94 (MQ=60)

                           GTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCC                                                                   <  SRR4302533.868186/94‑1 (MQ=60)

                            TGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCT                                                                  <  SRR4302533.2218367/94‑1 (MQ=60)

                            TGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCT                                                                  <  SRR4302533.1840418/94‑1 (MQ=60)

                             GAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTT                                                                 >  SRR4302533.1639528/1‑94 (MQ=60)

                               GCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTAT                                                               <  SRR4302533.5258909/94‑1 (MQ=60)

                               GCTCGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGAAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTAT                                                               >  SRR4302533.1657775/1‑94 (MQ=60)

                                CTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATA                                                              <  SRR4302533.2390369/94‑1 (MQ=60)

                                CTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATA                                                              >  SRR4302533.3275909/1‑94 (MQ=60)

                                 TGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATAC                                                             <  SRR4302533.5136586/94‑1 (MQ=60)

                                 TGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATAC                                                             <  SRR4302533.833017/94‑1 (MQ=60)

                                  GGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACC                                                            >  SRR4302533.2074992/1‑94 (MQ=60)

                                   GAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCG                                                           <  SRR4302533.1110929/94‑1 (MQ=60)

                                    AAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGG                                                          >  SRR4302533.1922997/1‑94 (MQ=60)

                                     AGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGT                                                         >  SRR4302533.2741989/1‑94 (MQ=60)

                                         CAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATA                                                     <  SRR4302533.743652/94‑1 (MQ=60)

                                          AGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATAT                                                    >  SRR4302533.1716472/1‑94 (MQ=60)

                                          AGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATAT                                                    <  SRR4302533.2309923/94‑1 (MQ=60)

                                           GGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATC                                                   <  SRR4302533.2629895/94‑1 (MQ=60)

                                           GGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATC                                                   >  SRR4302533.2036061/1‑94 (MQ=60)

                                           GGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATC                                                   >  SRR4302533.1990687/1‑94 (MQ=60)

                                           GGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATC                                                   >  SRR4302533.6148033/1‑94 (MQ=60)

                                            GGCGATATTCAGGTGGTGCAAAGCTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCG                                                  <  SRR4302533.4907845/94‑1 (MQ=60)

                                            GGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCG                                                  >  SRR4302533.5044601/1‑94 (MQ=60)

                                              CGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCC                                                <  SRR4302533.2989918/94‑1 (MQ=60)

                                              CGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCC                                                >  SRR4302533.5884799/1‑94 (MQ=60)

                                               GATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGTCCCCTTATACCGGTGATATCGCCG                                               <  SRR4302533.3282888/94‑1 (MQ=60)

                                                ATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGC                                              >  SRR4302533.81942/1‑94 (MQ=60)

                                                ATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGC                                              >  SRR4302533.2187574/1‑94 (MQ=60)

                                                  ATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTG                                            >  SRR4302533.1770551/1‑94 (MQ=60)

                                                    TCAGGTGGTGCAAAACCTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAA                                          <  SRR4302533.3726804/94‑1 (MQ=60)

                                                     TAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGCAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAG                                         <  SRR4302533.4102556/94‑1 (MQ=60)

                                                        GTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAA                                      >  SRR4302533.2065981/1‑94 (MQ=60)

                                                          GGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATC                                    <  SRR4302533.4879736/94‑1 (MQ=60)

                                                          GGTGCAAAACGTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCTGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATC                                    <  SRR4302533.3093048/94‑1 (MQ=60)

                                                           GTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCA                                   <  SRR4302533.4439848/94‑1 (MQ=60)

                                                           GTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCA                                   <  SRR4302533.4815719/94‑1 (MQ=60)

                                                           GTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCA                                   >  SRR4302533.5900347/1‑94 (MQ=60)

                                                            TGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAG                                  >  SRR4302533.1736662/1‑94 (MQ=60)

                                                            TGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAG                                  >  SRR4302533.4680183/1‑94 (MQ=60)

                                                            TGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAG                                  >  SRR4302533.1805515/1‑94 (MQ=60)

                                                             GCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGC                                 <  SRR4302533.4588854/94‑1 (MQ=60)

                                                             GCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGC                                 <  SRR4302533.1714253/94‑1 (MQ=60)

                                                             GCAAAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGC                                 >  SRR4302533.807526/1‑94 (MQ=60)

                                                                AAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAA                              >  SRR4302533.4169662/1‑94 (MQ=60)

                                                                AAACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAA                              <  SRR4302533.1063517/94‑1 (MQ=60)

                                                                 aactttcgtgcggtggcaggtccGGCAGGGTTCGGCCCTGCGGAACTGCCGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAG                             <  SRR4302533.5210554/71‑1 (MQ=60)

                                                                  ACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGC                            <  SRR4302533.1840394/94‑1 (MQ=60)

                                                                  ACTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGC                            <  SRR4302533.3616470/94‑1 (MQ=60)

                                                                   CTTCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCC                           <  SRR4302533.800400/94‑1 (MQ=60)

                                                                     TCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGTCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCAT                         <  SRR4302533.2025526/94‑1 (MQ=60)

                                                                     TCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCAT                         <  SRR4302533.1534323/94‑1 (MQ=60)

                                                                     TCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCAT                         <  SRR4302533.3498269/94‑1 (MQ=60)

                                                                     TCGTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCAT                         <  SRR4302533.1934128/94‑1 (MQ=60)

                                                                       GTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGC                       <  SRR4302533.5474576/94‑1 (MQ=60)

                                                                       GTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGC                       <  SRR4302533.5899445/94‑1 (MQ=60)

                                                                       GTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGC                       <  SRR4302533.1617227/94‑1 (MQ=60)

                                                                       GTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGC                       <  SRR4302533.858706/94‑1 (MQ=60)

                                                                       GTTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGC                       >  SRR4302533.1935285/1‑94 (MQ=60)

                                                                        TTGCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCG                      <  SRR4302533.4593351/94‑1 (MQ=60)

                                                                          GCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCG                    <  SRR4302533.3552033/94‑1 (MQ=60)

                                                                          GCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCG                    <  SRR4302533.4145185/94‑1 (MQ=60)

                                                                          GCGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCG                    <  SRR4302533.2524203/94‑1 (MQ=60)

                                                                           CGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGCAGGAATCAGCAAAGCCATGCGCGG                   >  SRR4302533.1802299/1‑94 (MQ=60)

                                                                           CGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGG                   <  SRR4302533.2885018/94‑1 (MQ=60)

                                                                           CGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGG                   >  SRR4302533.3559777/1‑94 (MQ=60)

                                                                           CGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGG                   <  SRR4302533.4354404/94‑1 (MQ=60)

                                                                           CGCTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGG                   >  SRR4302533.5111057/1‑94 (MQ=60)

                                                                             CTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAG                 <  SRR4302533.4259586/94‑1 (MQ=60)

                                                                             CTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAG                 >  SRR4302533.3161043/1‑94 (MQ=60)

                                                                             CTGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAG                 <  SRR4302533.2660058/94‑1 (MQ=60)

                                                                              TGGCAGATCTGGGAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAGG                >  SRR4302533.134582/1‑94 (MQ=60)

                                                                              TGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAGG                >  SRR4302533.1389604/1‑94 (MQ=60)

                                                                              TGGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAGG                <  SRR4302533.176060/94‑1 (MQ=60)

                                                                               CGCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAGGC               <  SRR4302533.5345648/94‑1 (MQ=60)

                                                                                GCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAGGCG              <  SRR4302533.5943321/94‑1 (MQ=60)

                                                                                GCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAGGCG              >  SRR4302533.5800537/1‑94 (MQ=60)

                                                                                GCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAGGCG              >  SRR4302533.2305821/1‑94 (MQ=60)

                                                                                GCAGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAGGCG              <  SRR4302533.2994129/94‑1 (MQ=60)

                                                                                  AGATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAGGCGCA            <  SRR4302533.9756/94‑1 (MQ=60)

                                                                                   GATCTGGCAGGGTTCGACCCTGCGGAACTGTTGGCTCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAGGCGCAA           <  SRR4302533.141470/94‑1 (MQ=60)

                                                                                   GATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAGGCGCAA           <  SRR4302533.5074540/94‑1 (MQ=60)

                                                                                    ATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATCTCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAGGCGCAAA          <  SRR4302533.4459627/94‑1 (MQ=60)

                                                                                    ATCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAGGCGCAAA          >  SRR4302533.4089602/1‑94 (MQ=60)

                                                                                     TCTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAGGCGCAAAG         >  SRR4302533.2539280/1‑94 (MQ=60)

                                                                                     TCTGGCAGGGTTCGACCCTGCGGAACTGCTGACCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAGGCGCAAAG         <  SRR4302533.2768055/94‑1 (MQ=60)

                                                                                      CTGGCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAGGCGCAAAGT        >  SRR4302533.5456315/1‑94 (MQ=60)

                                                                                         GCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAGGCGCAAAGTTCC     <  SRR4302533.2956484/94‑1 (MQ=60)

                                                                                         GCAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAGGCGCAAAGTTCC     <  SRR4302533.4560995/94‑1 (MQ=60)

                                                                                          CAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAGGCGCAAAGTTCCT    >  SRR4302533.2518600/1‑94 (MQ=60)

                                                                                          CAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAGGCGCAAAGTTCCT    <  SRR4302533.3151640/94‑1 (MQ=60)

                                                                                          CAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAGGCGCAAAGTTCCT    <  SRR4302533.5059091/94‑1 (MQ=60)

                                                                                          CAGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAGGCGCAAAGTTCCT    >  SRR4302533.4779285/1‑94 (MQ=60)

                                                                                           AGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATGCCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAGGCGCAAAGTTCCTG   <  SRR4302533.1856825/94‑1 (MQ=60)

                                                                                           AGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAGGCGCAGAGTTCCTG   >  SRR4302533.2084830/1‑94 (MQ=60)

                                                                                           AGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAGGCGCAAAGTTCCTG   <  SRR4302533.5856887/94‑1 (MQ=60)

                                                                                           AGGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAGGCGCAAAGTTCCTG   >  SRR4302533.4904346/1‑94 (MQ=60)

                                                                                            GGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAGGCGCAAAGTTCCTGC  >  SRR4302533.2722793/1‑94 (MQ=60)

                                                                                            GGGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAGGCGCAAAGTTCCTGC  >  SRR4302533.1980894/1‑94 (MQ=60)

AGCAGCTTACCGCACTGATTCGCAGTGGTGAGCTGGAAGTGCAGGGCGATATTCAGGTGGTGCAAAACTTCGTTGCGCTGGCAGATCTGGCAGAGTTCGACCCTGCGGAACTGCTGGCCCCTTATACCGGTGATATCGCCGCTGAAGGAATCAGCAAAGCCATGCGCGGAGGCGCAAAGTTCCTGC  >  NC_000913/4017464‑4017649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`