Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,031,984	+C	coding (1250/1299 nt)	trkH →	TrkH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,031,983	1	.	C	99.5%	696.2 / ‑5.7	220	A417P (GCA→CCA)	trkH	TrkH
Reads supporting (aligned to +/- strand): ref base . (1/0); major base C (117/101); minor base T (1/0); total (119/101)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 238 total aligned reads displayed.

ATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑CACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATA  >  NC_000913/4031891‑4032069

aTCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCg                                                                                         <  1:1005081/93‑1 (MQ=255)

 tCGCGCACTGCGGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑c                                                                                       <  2:444333/93‑1 (MQ=255)

 tCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑c                                                                                       >  2:3620580/1‑93 (MQ=255)

 tCGCGCACTGCCGAAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑c                                                                                       <  2:5710051/93‑1 (MQ=255)

   gcgcACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑cca                                                                                     <  2:2263025/93‑3 (MQ=255)

     gcACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑ccacg                                                                                   >  2:6032196/1‑89 (MQ=255)

       aCTGCCGCAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACggg                                                                                  <  1:5097037/93‑1 (MQ=255)

        cTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGc                                                                                 >  1:2710761/1‑93 (MQ=255)

         tGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCg                                                                                >  2:2324205/1‑93 (MQ=255)

          gCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCgg                                                                               >  2:3904696/1‑92 (MQ=255)

            cGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTgg                                                                             <  2:140607/93‑1 (MQ=255)

            cGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTgg                                                                             <  1:2567556/93‑1 (MQ=255)

                aCGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGa                                                                         >  1:4627569/1‑93 (MQ=255)

                 cGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGAc                                                                        <  2:1862446/93‑1 (MQ=255)

                  gTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACt                                                                       >  1:413492/1‑93 (MQ=255)

                  gTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACt                                                                       >  2:3372891/1‑93 (MQ=255)

                   tATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACtt                                                                      >  1:2618698/1‑93 (MQ=255)

                    aTCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACttt                                                                     >  2:3193618/1‑93 (MQ=255)

                     tCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACtttt                                                                    >  1:1760255/1‑93 (MQ=255)

                      ccTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTc                                                                   <  1:5844459/93‑1 (MQ=255)

                       cTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCt                                                                  >  2:5065235/1‑93 (MQ=255)

                        tCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTg                                                                 >  1:5200012/1‑93 (MQ=255)

                          gAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGcc                                                               >  1:1466819/1‑93 (MQ=255)

                           aaGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCt                                                              <  2:529943/93‑1 (MQ=255)

                           aaGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCt                                                              <  2:2683033/93‑1 (MQ=255)

                            aGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCtt                                                             >  2:3854664/1‑93 (MQ=255)

                             gCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCttt                                                            >  2:5614093/1‑93 (MQ=255)

                             gCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGGTTATTGTCAGTATGCTGGCGATTATCGTCACGGGCGTGGATGACTTTTCTGCCttt                                                            >  2:4770055/1‑93 (MQ=255)

                               cGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGc                                                          <  1:2956620/93‑1 (MQ=255)

                               cGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGc                                                          >  2:646008/1‑93 (MQ=255)

                               cGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGc                                                          <  1:2117946/93‑1 (MQ=255)

                                gTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCg                                                         >  1:3537002/1‑93 (MQ=255)

                                gTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCg                                                         >  2:4302860/1‑93 (MQ=255)

                                gTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCg                                                         <  1:2928427/93‑1 (MQ=255)

                                gTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCg                                                         <  2:801434/93‑1 (MQ=255)

                                 tttGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGt                                                        <  1:1134752/93‑1 (MQ=255)

                                 tttGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGt                                                        >  1:6007242/1‑93 (MQ=255)

                                  ttGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTc                                                       >  1:3260750/1‑93 (MQ=255)

                                  ttGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTc                                                       >  1:4911805/1‑93 (MQ=255)

                                  ttGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTc                                                       >  2:1034735/1‑93 (MQ=255)

                                   tGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCg                                                      >  1:14005/1‑93 (MQ=255)

                                   tGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCg                                                      <  2:5270725/93‑1 (MQ=255)

                                   tGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCg                                                      <  2:3303764/93‑1 (MQ=255)

                                    tgggATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCgg                                                     <  2:4712691/92‑1 (MQ=255)

                                    ggggATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCgg                                                     >  2:5824269/1‑93 (MQ=255)

                                     gggATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGgt                                                    <  2:2796412/93‑1 (MQ=255)

                                      ggATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGgtt                                                   <  1:536824/93‑1 (MQ=255)

                                        aTTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGgttgt                                                 <  1:1233039/93‑1 (MQ=255)

                                          ttttCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATAGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGttgttg                                               >  1:4018700/1‑93 (MQ=255)

                                             tctcCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGa                                            <  1:2220125/93‑1 (MQ=255)

                                             tctcCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGa                                            >  2:5942862/1‑93 (MQ=255)

                                              ctcCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGAc                                           <  1:243756/93‑1 (MQ=255)

                                               tcCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACa                                          >  2:880862/1‑93 (MQ=255)

                                               tcCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACa                                          >  2:2129287/1‑93 (MQ=255)

                                               tcCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACa                                          <  1:5810943/93‑1 (MQ=255)

                                                ccGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGTGTCCGTTGTTGCGACAt                                         >  1:96088/1‑93 (MQ=255)

                                                 cGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACAtt                                        <  2:1809327/93‑1 (MQ=255)

                                                 cGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACAtt                                        <  1:4106817/93‑1 (MQ=255)

                                                 cGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACAtt                                        <  1:237750/93‑1 (MQ=255)

                                                  gCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTg                                       <  1:859011/93‑1 (MQ=255)

                                                   ccTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGa                                      >  1:5738615/1‑93 (MQ=255)

                                                    cTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGaa                                     <  2:2903534/93‑1 (MQ=255)

                                                    cTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGaa                                     <  2:1527917/93‑1 (MQ=255)

                                                     tATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAAt                                    <  1:1822130/93‑1 (MQ=255)

                                                     tATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAAt                                    >  2:3164454/1‑93 (MQ=255)

                                                      aTGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATa                                   <  2:6248801/93‑1 (MQ=255)

                                                       tGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATaa                                  >  1:3485595/1‑93 (MQ=255)

                                                       tGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATaa                                  >  1:1612422/1‑93 (MQ=255)

                                                        gCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAAc                                 >  2:2508857/1‑93 (MQ=255)

                                                        gCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAAc                                 <  2:3448234/93‑1 (MQ=255)

                                                        gCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAAc                                 >  2:3561145/1‑93 (MQ=255)

                                                         gaTTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAAcc                                <  1:283985/92‑1 (MQ=255)

                                                         cATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAAcc                                <  1:3593239/93‑1 (MQ=255)

                                                         cATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAAcc                                <  2:161953/93‑1 (MQ=255)

                                                          aTTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCt                               <  2:5220305/93‑1 (MQ=255)

                                                          aTTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCt                               >  1:4570944/1‑93 (MQ=255)

                                                          aTTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGGTGTTGCGGCCTTGAATACCCt                               >  2:4546657/1‑93 (MQ=255)

                                                            tGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTAGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTgg                             <  2:1343150/93‑1 (MQ=255)

                                                             ggTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTggg                            <  2:3748772/93‑1 (MQ=255)

                                                             ggTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTggg                            <  2:1194968/93‑1 (MQ=255)

                                                              gtgtTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTgggg                           <  2:2202042/93‑1 (MQ=255)

                                                               tgtTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGGCTTTTCTGCCTTTGCGTCGGTTGTTTCCACCTTGAATAACCTAGGGc                          >  2:3504383/1‑93 (MQ=255)

                                                               tgtTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGc                          <  2:1270068/93‑1 (MQ=255)

                                                               tgtTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGc                          >  1:2311690/1‑93 (MQ=255)

                                                                gtTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGcc                         <  2:4852013/93‑1 (MQ=255)

                                                                       gTCAGTATGCGGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCGTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCtt                  <  2:2977619/93‑1 (MQ=255)

                                                                         cAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTgg                <  1:426365/93‑1 (MQ=255)

                                                                          aGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGc               >  1:1882124/1‑93 (MQ=255)

                                                                          aGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGc               <  2:2402793/93‑1 (MQ=255)

                                                                           gTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCg              <  1:251018/93‑1 (MQ=255)

                                                                             aTGCTGGCGATTATCGCCACGGGCGTGGATGACTATTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGCGCTTGGCGTg            >  2:4830623/1‑93 (MQ=255)

                                                                              tGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTgg           >  2:2481699/1‑93 (MQ=255)

                                                                              tGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTgg           <  1:6117229/93‑1 (MQ=255)

                                                                               gCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGt          <  1:3917099/93‑1 (MQ=255)

                                                                                cTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGtt         <  1:4751260/93‑1 (MQ=255)

                                                                                 tGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTg        >  1:2660389/1‑93 (MQ=255)

                                                                                  ggCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGc       <  1:3164454/93‑1 (MQ=255)

                                                                                   gCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCt      >  1:5455882/1‑93 (MQ=255)

                                                                                   gCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCt      <  2:6185922/93‑1 (MQ=255)

                                                                                   gCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCt      >  2:5172168/1‑93 (MQ=255)

ATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑CACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATA  >  NC_000913/4031891‑4032069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑CACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACTTTACC  >  NC_000913/4031891‑4032077

ATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑C                                                                                               <  SRR4302533.1010959/94‑1 (MQ=60)

ATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑C                                                                                               <  SRR4302533.2038964/94‑1 (MQ=60)

ATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑C                                                                                               <  SRR4302533.5798779/94‑1 (MQ=60)

 TCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑CC                                                                                              >  SRR4302533.5130444/1‑94 (MQ=60)

  CGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑Cca                                                                                             >  SRR4302533.4903937/1‑92 (MQ=60)

  CGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGATGGCGATTATCG‑Cca                                                                                             >  SRR4302533.5444257/1‑92 (MQ=60)

    CGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACG                                                                                            <  SRR4302533.420968/94‑1 (MQ=60)

      CACTGCCGGAACGTATCCTCGACGCCGTTTGGGGCTTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGG                                                                                          >  SRR4302533.1082009/1‑94 (MQ=60)

      CACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGG                                                                                          <  SRR4302533.3199451/94‑1 (MQ=60)

      CACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGG                                                                                          <  SRR4302533.230043/94‑1 (MQ=60)

      CACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTGCTCCGCCTATGCATTGGTATTTATTGTCAGTATGCTGGCGATTATCGCCACGGG                                                                                          <  SRR4302533.1651068/94‑1 (MQ=60)

       ACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGC                                                                                         >  SRR4302533.3283202/1‑94 (MQ=60)

       ACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGC                                                                                         <  SRR4302533.263655/94‑1 (MQ=60)

       ACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGC                                                                                         >  SRR4302533.2727400/1‑94 (MQ=60)

       ACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGC                                                                                         >  SRR4302533.4150248/1‑94 (MQ=60)

       ACTGCCGCAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGC                                                                                         <  SRR4302533.5127831/94‑1 (MQ=60)

           CGGGAACGTATCCTCGAAGCCGTTTGGCGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGG                                                                                     <  SRR4302533.3137154/94‑1 (MQ=60)

           CCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGG                                                                                     <  SRR4302533.2220191/94‑1 (MQ=60)

            CGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGA                                                                                    <  SRR4302533.2583356/94‑1 (MQ=60)

            CGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGA                                                                                    >  SRR4302533.4323919/1‑94 (MQ=60)

             GGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGAT                                                                                   <  SRR4302533.1855002/94‑1 (MQ=60)

              GAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATG                                                                                  >  SRR4302533.4066505/1‑94 (MQ=60)

               AACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGA                                                                                 >  SRR4302533.4655616/1‑94 (MQ=60)

                 CGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACT                                                                               >  SRR4302533.1861980/1‑94 (MQ=60)

                 CGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACT                                                                               >  SRR4302533.415973/1‑94 (MQ=60)

                  GTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTT                                                                              >  SRR4302533.4848459/1‑94 (MQ=60)

                  GTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTT                                                                              >  SRR4302533.2634774/1‑94 (MQ=60)

                   TATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTT                                                                             >  SRR4302533.351866/1‑94 (MQ=60)

                    ATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTT                                                                            >  SRR4302533.1770981/1‑94 (MQ=60)

                    ATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTT                                                                            >  SRR4302533.1588955/1‑94 (MQ=60)

                      CCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCT                                                                          <  SRR4302533.5879792/94‑1 (MQ=60)

                      CCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCT                                                                          >  SRR4302533.1678351/1‑94 (MQ=60)

                      CCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCT                                                                          <  SRR4302533.5199213/94‑1 (MQ=60)

                       CTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTG                                                                         >  SRR4302533.5231361/1‑94 (MQ=60)

                        TCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGC                                                                        <  SRR4302533.2312295/94‑1 (MQ=60)

                         CGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCC                                                                       <  SRR4302533.1167823/94‑1 (MQ=60)

                         CGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCC                                                                       >  SRR4302533.1475664/1‑94 (MQ=60)

                          GAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCT                                                                      >  SRR4302533.1817053/1‑94 (MQ=60)

                             GCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTG                                                                   >  SRR4302533.3272833/1‑94 (MQ=60)

                               CGTTTGGGGATTTTTCTCCTCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCG                                                                 <  SRR4302533.2708801/94‑1 (MQ=60)

                               CGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCG                                                                 >  SRR4302533.3558801/1‑94 (MQ=60)

                               CGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCG                                                                 <  SRR4302533.2974693/94‑1 (MQ=60)

                               CGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCG                                                                 <  SRR4302533.2131008/94‑1 (MQ=60)

                               CGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCG                                                                 >  SRR4302533.5713438/1‑94 (MQ=60)

                                GTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGT                                                                <  SRR4302533.2946340/94‑1 (MQ=60)

                                GTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGT                                                                >  SRR4302533.6043636/1‑94 (MQ=60)

                                 TTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTC                                                               >  SRR4302533.3280865/1‑94 (MQ=60)

                                 TTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTC                                                               >  SRR4302533.4941559/1‑94 (MQ=60)

                                 TTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTC                                                               <  SRR4302533.1141499/94‑1 (MQ=60)

                                  TTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCG                                                              >  SRR4302533.14109/1‑94 (MQ=60)

                                   TGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGG                                                             >  SRR4302533.749856/1‑94 (MQ=60)

                                    GGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGTATGACTTTTCTGCCTTTGCGTCGGT                                                            <  SRR4302533.2486699/94‑1 (MQ=60)

                                     GGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTT                                                           >  SRR4302533.5849637/1‑94 (MQ=60)

                                      GGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTG                                                          <  SRR4302533.540014/94‑1 (MQ=60)

                                       GATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGT                                                         <  SRR4302533.144700/94‑1 (MQ=60)

                                        ATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTT                                                        <  SRR4302533.1240417/94‑1 (MQ=60)

                                        ATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGAGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTT                                                        <  SRR4302533.6089800/94‑1 (MQ=60)

                                         TTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATAGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTG                                                       >  SRR4302533.4043171/1‑94 (MQ=60)

                                          TTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGC                                                      <  SRR4302533.1654012/94‑1 (MQ=60)

                                          TTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGC                                                      >  SRR4302533.259959/1‑94 (MQ=60)

                                           TTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCG                                                     <  SRR4302533.99467/94‑1 (MQ=60)

                                           TTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCG                                                     <  SRR4302533.3712844/94‑1 (MQ=60)

                                             TCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGAC                                                   <  SRR4302533.2233830/94‑1 (MQ=60)

                                             TCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGAC                                                   >  SRR4302533.2288729/1‑94 (MQ=60)

                                              CTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACA                                                  <  SRR4302533.245268/94‑1 (MQ=60)

                                              CTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACA                                                  <  SRR4302533.3732524/94‑1 (MQ=60)

                                               TCCGCGTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACAT                                                 <  SRR4302533.5957018/94‑1 (MQ=60)

                                               TCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGTGTCCGTTGTTGCGACAT                                                 >  SRR4302533.96691/1‑94 (MQ=60)

                                               TCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACAT                                                 <  SRR4302533.4978691/94‑1 (MQ=60)

                                               TCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACAT                                                 <  SRR4302533.5846035/94‑1 (MQ=60)

                                                CCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATT                                                >  SRR4302533.2553699/1‑94 (MQ=60)

                                                CCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATT                                                >  SRR4302533.2583580/1‑94 (MQ=60)

                                                 CGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTG                                               <  SRR4302533.239222/94‑1 (MQ=60)

                                                 CGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTG                                               <  SRR4302533.769302/94‑1 (MQ=60)

                                                 CGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTG                                               <  SRR4302533.4131789/94‑1 (MQ=60)

                                                  GCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGA                                              >  SRR4302533.5773248/1‑94 (MQ=60)

                                                  GCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGA                                              <  SRR4302533.864045/94‑1 (MQ=60)

                                                   CCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAA                                             <  SRR4302533.2392023/94‑1 (MQ=60)

                                                     TATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATA                                           <  SRR4302533.1833261/94‑1 (MQ=60)

                                                      ATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAA                                          >  SRR4302533.1622197/1‑94 (MQ=60)

                                                      ATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAA                                          >  SRR4302533.3507091/1‑94 (MQ=60)

                                                         GATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCT                                       <  SRR4302533.285722/94‑1 (MQ=60)

                                                         CATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCT                                       <  SRR4302533.3615351/94‑1 (MQ=60)

                                                         CATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCT                                       >  SRR4302533.962693/1‑94 (MQ=60)

                                                         CATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCT                                       <  SRR4302533.5311015/94‑1 (MQ=60)

                                                         CATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCT                                       >  SRR4302533.4598647/1‑94 (MQ=60)

                                                           TTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGG                                     >  SRR4302533.1325970/1‑94 (MQ=60)

                                                           TTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGG                                     >  SRR4302533.2940391/1‑94 (MQ=60)

                                                            TGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGG                                    >  SRR4302533.3433078/1‑94 (MQ=60)

                                                            TGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGG                                    <  SRR4302533.469300/94‑1 (MQ=60)

                                                            TGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGG                                    <  SRR4302533.2508383/94‑1 (MQ=60)

                                                             GGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGG                                   <  SRR4302533.2786825/94‑1 (MQ=60)

                                                              GTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGC                                  >  SRR4302533.2326011/1‑94 (MQ=60)

                                                                GTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCA                                >  SRR4302533.4074035/1‑94 (MQ=60)

                                                                 TTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAG                               <  SRR4302533.2425331/94‑1 (MQ=60)

                                                                    ATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGC                            >  SRR4302533.6066937/1‑94 (MQ=60)

                                                                       GTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTG                         >  SRR4302533.4606592/1‑94 (MQ=60)

                                                                        TCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGG                        <  SRR4302533.3583039/94‑1 (MQ=60)

                                                                         CAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGC                       >  SRR4302533.1893584/1‑94 (MQ=60)

                                                                         CAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGC                       <  SRR4302533.428908/94‑1 (MQ=60)

                                                                         CAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGC                       >  SRR4302533.3981146/1‑94 (MQ=60)

                                                                           GTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGT                     <  SRR4302533.252565/94‑1 (MQ=60)

                                                                             ATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGG                   >  SRR4302533.1348244/1‑94 (MQ=60)

                                                                              TGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGT                  <  SRR4302533.6154129/94‑1 (MQ=60)

                                                                               GCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTT                 <  SRR4302533.3941023/94‑1 (MQ=60)

                                                                               GCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCCGGGGCCAGGGCTTGGCGTGGTT                 >  SRR4302533.5699101/1‑94 (MQ=60)

                                                                                CTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTG                >  SRR4302533.2676725/1‑94 (MQ=60)

                                                                                CTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTG                <  SRR4302533.4780066/94‑1 (MQ=60)

                                                                                  GGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCT              >  SRR4302533.5488751/1‑94 (MQ=60)

                                                                                  GGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCT              <  SRR4302533.3183930/94‑1 (MQ=60)

                                                                                   GCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTG             <  SRR4302533.2803658/94‑1 (MQ=60)

                                                                                     GATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGAT           >  SRR4302533.5888483/1‑94 (MQ=60)

                                                                                     GATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGAT           >  SRR4302533.2965581/1‑94 (MQ=60)

                                                                                     GATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGCTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGAT           >  SRR4302533.4537015/1‑94 (MQ=60)

                                                                                      ATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATA          >  SRR4302533.2997840/1‑94 (MQ=60)

                                                                                        TATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAAC        >  SRR4302533.5207498/1‑94 (MQ=60)

                                                                                         ATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACT       <  SRR4302533.4150364/94‑1 (MQ=60)

                                                                                           cgcCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACTTT     <  SRR4302533.216386/91‑1 (MQ=60)

                                                                                           cgcCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACTTT     <  SRR4302533.1594316/91‑1 (MQ=60)

                                                                                            gcCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACTTTA    >  SRR4302533.1308/3‑94 (MQ=60)

                                                                                            gcCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACTTTA    >  SRR4302533.4873817/3‑94 (MQ=60)

                                                                                              CACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACTTTACC  <  SRR4302533.1086054/94‑1 (MQ=60)

ATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑CACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACTTTACC  >  NC_000913/4031891‑4032077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`