Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,031,984 +C coding (1250/1299 nt) trkH → TrkH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,031,9831.C99.8% 1400.3 / ‑4.5 435coding (1249/1299 nt)trkHTrkH
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base C (264/170);  total (264/171)
Fisher's exact test for biased strand distribution p-value = 3.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 477 total aligned reads displayed.

ATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑C‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAA  >  NC_000913/4031891‑4032070
                                                                                             |                                                                                        
 tCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑c                                                                                         <  2:2356657/93‑1 (MQ=255)
 tCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑c                                                                                         >  1:3627048/1‑93 (MQ=255)
   gcgcACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑cca                                                                                       >  2:3934820/1‑91 (MQ=255)
   gcgcACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑cca                                                                                       >  2:6762247/1‑91 (MQ=255)
   gcgcACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑cca                                                                                       <  1:1967055/93‑3 (MQ=255)
         tGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCg                                                                                 >  1:2625900/1‑93 (MQ=255)
         tGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCg                                                                                 >  2:4914329/1‑93 (MQ=255)
         tGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCg                                                                                 >  2:6287969/1‑93 (MQ=255)
          gCCGTAACGCATGCTTGAACCTGTGTGGGGATTTTTCTCCTCCTTTCAATTGGTGTTTATTGTCAGTATGCTGGCCATTATCGCC‑ACGGGCGt                                                                                <  2:3228112/93‑1 (MQ=255)
          gCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGt                                                                                <  2:6149247/93‑1 (MQ=255)
           ccGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTg                                                                               >  1:4508122/1‑93 (MQ=255)
            cGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCgggg                                                                              >  2:4665008/1‑93 (MQ=255)
             ggAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGa                                                                             >  2:9869702/1‑93 (MQ=255)
             ggAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGa                                                                             >  1:9750702/1‑93 (MQ=255)
             ggAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGa                                                                             <  1:5588835/93‑1 (MQ=255)
              gAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGAt                                                                            >  1:8818223/1‑93 (MQ=255)
                aCGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGa                                                                          >  2:4349621/1‑93 (MQ=255)
                   tATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACtt                                                                       <  1:779745/93‑1 (MQ=255)
                    aTCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACttt                                                                      >  1:7252523/1‑93 (MQ=255)
                    aTCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACttt                                                                      >  1:8650363/1‑93 (MQ=255)
                    aTCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACttt                                                                      <  1:7606811/93‑1 (MQ=255)
                      ccTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTc                                                                    <  1:4823398/93‑1 (MQ=255)
                       cTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCt                                                                   >  2:5225800/1‑93 (MQ=255)
                           aaGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCt                                                               <  2:3765523/93‑1 (MQ=255)
                            aGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCtt                                                              <  2:1361577/93‑1 (MQ=255)
                             gCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCttt                                                             >  1:7004174/1‑93 (MQ=255)
                             gCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCttt                                                             <  2:420234/93‑1 (MQ=255)
                               cGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGc                                                           <  1:2981310/93‑1 (MQ=255)
                                gTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCg                                                          >  1:5326494/1‑93 (MQ=255)
                                gTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCg                                                          <  2:930021/93‑1 (MQ=255)
                                gTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCg                                                          >  2:7378673/1‑93 (MQ=255)
                                gTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTCCCTTTGCg                                                          >  2:504477/1‑93 (MQ=255)
                                 tttGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATTACTTTTCTGCCTTTGCGt                                                         >  2:7467775/1‑93 (MQ=255)
                                 tttGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGt                                                         <  2:3425327/93‑1 (MQ=255)
                                 tttGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGt                                                         >  1:10016115/1‑93 (MQ=255)
                                   tGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCg                                                       >  2:5540864/1‑93 (MQ=255)
                                     gggATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGgt                                                     <  2:8731167/93‑1 (MQ=255)
                                      ggATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGgtt                                                    >  1:4400448/1‑93 (MQ=255)
                                       gATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGgttg                                                   >  1:2364531/1‑93 (MQ=255)
                                       gATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGgttg                                                   <  1:312009/93‑1 (MQ=255)
                                        aTTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGgtttt                                                  <  2:8397274/93‑3 (MQ=255)
                                        aTTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGgttgt                                                  <  2:6433304/93‑1 (MQ=255)
                                         tttttCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGgttgtt                                                 >  1:7365908/1‑93 (MQ=255)
                                          ttttCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGttgttg                                                >  1:4204285/1‑93 (MQ=255)
                                           tttCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGc                                               >  1:8156715/1‑93 (MQ=255)
                                              ctcCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGAc                                            <  1:6272622/93‑1 (MQ=255)
                                               tcCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACa                                           >  1:4994507/1‑93 (MQ=255)
                                                  gCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTg                                        <  1:1523473/93‑1 (MQ=255)
                                                   ccTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGa                                       <  1:6614852/93‑1 (MQ=255)
                                                   ccTATGCATGGGTGTTTATTGTCAGTATGCTGGCGAGTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGa                                       <  1:6067645/93‑1 (MQ=255)
                                                    ctttGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑CTACGTGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTCCGACATTGaa                                      <  2:8538798/90‑1 (MQ=255)
                                                    cTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGaa                                      <  2:213830/93‑1 (MQ=255)
                                                     tATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAAt                                     >  2:7269039/1‑93 (MQ=255)
                                                      aTGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATa                                    >  1:1408258/1‑93 (MQ=255)
                                                       tGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATaa                                   >  2:7725766/1‑93 (MQ=255)
                                                       tGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATaa                                   >  2:3540657/1‑93 (MQ=255)
                                                       tGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATaa                                   >  1:8770492/1‑93 (MQ=255)
                                                        gCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAAc                                  <  1:6466263/93‑1 (MQ=255)
                                                          aTTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCt                                <  2:2510103/93‑1 (MQ=255)
                                                           ttGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTg                               <  1:8401854/93‑1 (MQ=255)
                                                           ttGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTg                               >  1:4340921/1‑93 (MQ=255)
                                                            tGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTgg                              >  1:7953636/1‑93 (MQ=255)
                                                             ggTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTggg                             >  1:9559499/1‑93 (MQ=255)
                                                               tgtTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGc                           >  1:2458652/1‑93 (MQ=255)
                                                               tgtTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGc                           >  1:1737517/1‑93 (MQ=255)
                                                               tgtTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGc                           >  2:7134814/1‑93 (MQ=255)
                                                               tgtTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTAGCGTCGGTTGTTGCGACATTGAATAACCTGGGGc                           >  2:1400445/1‑93 (MQ=255)
                                                                 tttATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCa                         <  2:916661/93‑1 (MQ=255)
                                                                  ttATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAg                        >  2:8005210/1‑93 (MQ=255)
                                                                   tATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAgg                       >  2:8136887/1‑93 (MQ=255)
                                                                   tATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAgg                       >  2:9475013/1‑93 (MQ=255)
                                                                    aTTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAggg                      >  2:8246324/1‑93 (MQ=255)
                                                                     ttGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGc                     <  2:6612569/93‑1 (MQ=255)
                                                                     ttGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGc                     >  1:1349684/1‑93 (MQ=255)
                                                                      tGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCt                    >  1:9333753/1‑93 (MQ=255)
                                                                       gTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCtt                   >  2:6918478/1‑93 (MQ=255)
                                                                       gTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCtt                   <  1:5085370/93‑1 (MQ=255)
                                                                        tCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTg                  >  2:2657822/1‑93 (MQ=255)
                                                                        tCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTg                  <  2:9006935/93‑1 (MQ=255)
                                                                         cAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTgg                 >  2:2775789/1‑93 (MQ=255)
                                                                          aGTATGCTGGCGATTATCGCC‑ACGGGAGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGc                >  2:1116270/1‑93 (MQ=255)
                                                                           gTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCg               <  2:2904752/93‑1 (MQ=255)
                                                                            tATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGt              <  2:9674172/93‑1 (MQ=255)
                                                                            gaTGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGt              <  1:3943858/92‑1 (MQ=255)
                                                                             aTGCTGGCGATTATCGCC‑ACGGGCGTGGCTGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGTGGACAGGGCTTGGCGTg             >  1:9514050/1‑93 (MQ=255)
                                                                             aTGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTg             <  1:8571785/93‑1 (MQ=255)
                                                                             aTGCTAGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTg             >  2:4479432/1‑93 (MQ=255)
                                                                              tGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTgg            >  2:3047151/1‑93 (MQ=255)
                                                                              tGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTgg            >  1:3378671/1‑93 (MQ=255)
                                                                              tGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTgg            >  1:9972515/1‑93 (MQ=255)
                                                                                cTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGtt          <  1:7026786/93‑1 (MQ=255)
                                                                                cTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGtt          >  1:9128671/1‑93 (MQ=255)
                                                                                 tGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTg         <  1:7495311/93‑1 (MQ=255)
                                                                                  ggCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGc        >  1:5243893/1‑93 (MQ=255)
                                                                                  ggCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGc        >  2:5833655/1‑93 (MQ=255)
                                                                                   gCGATTATCGCC‑ACGGGCGGCGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCt       <  2:1828498/93‑1 (MQ=255)
                                                                                    cGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTg      >  1:3572828/1‑93 (MQ=255)
                                                                                     gATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGa     >  1:376826/1‑93 (MQ=255)
                                                                                      aTTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTCGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGAt    >  1:8337619/1‑93 (MQ=255)
                                                                                        tATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATaa  >  2:169834/1‑93 (MQ=255)
                                                                                             |                                                                                        
ATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑C‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAA  >  NC_000913/4031891‑4032070

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 261 total aligned reads displayed.
ATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑CACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACTTTACC  >  NC_000913/4031891‑4032077
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ATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑C                                                                                               >  SRR4302540.3666742/1‑94 (MQ=60)
 TCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑CC                                                                                              >  SRR4302540.8875433/1‑94 (MQ=60)
   GCGCACTGCCGGACCGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑Ccac                                                                                            <  SRR4302540.5707183/94‑4 (MQ=60)
   GCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑Ccac                                                                                            >  SRR4302540.6596877/1‑91 (MQ=60)
   GCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑Ccac                                                                                            <  SRR4302540.1988422/94‑4 (MQ=60)
     GCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGG                                                                                           <  SRR4302540.7488913/94‑1 (MQ=60)
     GCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGG                                                                                           <  SRR4302540.3032777/94‑1 (MQ=60)
      ccgtcgcggatcgtctcctcacAGCCGTGTGGGTATTTTTCTCCGCCGATGTGTTGTAGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGG                                                                                          <  SRR4302540.4282537/72‑1 (MQ=20)
      CACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGG                                                                                          >  SRR4302540.8065894/1‑94 (MQ=60)
        CTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCG                                                                                        <  SRR4302540.942161/94‑1 (MQ=60)
        CTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCG                                                                                        >  SRR4302540.2654301/1‑94 (MQ=60)
        CTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCG                                                                                        >  SRR4302540.9762029/1‑94 (MQ=60)
        CTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCG                                                                                        >  SRR4302540.7980076/1‑94 (MQ=60)
         TGCCGGAACTGATCCTCGAAGCCGTTTGGGGATTTTTCTCCCCCTATGCATTGGTGTTTATTGTCAGTATGCGGGCGATTATCGCCACGGGCGT                                                                                       <  SRR4302540.2451223/94‑1 (MQ=60)
         TGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGT                                                                                       <  SRR4302540.2451222/94‑1 (MQ=60)
         TGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGT                                                                                       <  SRR4302540.3032864/94‑1 (MQ=60)
          TCCGGAACGTATCCTCGAAGCTGTTTGGGTATCTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCTATTATCGCCACGGGCGTG                                                                                      <  SRR4302540.4442652/94‑1 (MQ=60)
          GCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTG                                                                                      >  SRR4302540.6261295/1‑94 (MQ=60)
          GCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTG                                                                                      >  SRR4302540.7098181/1‑94 (MQ=60)
          GCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTG                                                                                      >  SRR4302540.4556974/1‑94 (MQ=60)
           CCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGG                                                                                     >  SRR4302540.1152967/1‑94 (MQ=60)
            CGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGA                                                                                    >  SRR4302540.4446792/1‑94 (MQ=60)
            CGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGA                                                                                    >  SRR4302540.9865120/1‑94 (MQ=60)
            CGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCCTGGA                                                                                    >  SRR4302540.1775847/1‑94 (MQ=60)
             GGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGAT                                                                                   >  SRR4302540.5493722/1‑94 (MQ=60)
             GGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGAT                                                                                   <  SRR4302540.5650343/94‑1 (MQ=60)
             GGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGAT                                                                                   >  SRR4302540.8920179/1‑94 (MQ=60)
             GGAACGTATCCGCGAAGCCGGTTGGGGATTTGTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGAT                                                                                   <  SRR4302540.9821905/94‑1 (MQ=60)
              GAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATG                                                                                  >  SRR4302540.1888830/1‑94 (MQ=60)
                 CGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACT                                                                               >  SRR4302540.9377551/1‑94 (MQ=60)
                  GTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTT                                                                              >  SRR4302540.8025586/1‑94 (MQ=60)
                  GTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTT                                                                              >  SRR4302540.3403910/1‑94 (MQ=60)
                   TATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTT                                                                             <  SRR4302540.9928569/94‑1 (MQ=60)
                   TATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTT                                                                             >  SRR4302540.3125842/1‑94 (MQ=60)
                   TATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTT                                                                             >  SRR4302540.7333456/1‑94 (MQ=60)
                   TATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTT                                                                             <  SRR4302540.788051/94‑1 (MQ=60)
                   TATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTT                                                                             <  SRR4302540.9928566/94‑1 (MQ=60)
                   TATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTT                                                                             >  SRR4302540.8750851/1‑94 (MQ=60)
                   TATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTT                                                                             <  SRR4302540.9508396/94‑1 (MQ=60)
                    CTCCTGGAAGCCGTTTGGGGATGTTTCGTCGCTTATGCATTGGTTTTTATTGTCAGTATGCTGGCGATGATCGCCACGGGCGTGGATGACTTTT                                                                            <  SRR4302540.1519691/94‑1 (MQ=55)
                    ATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTT                                                                            <  SRR4302540.7692290/94‑1 (MQ=60)
                    ATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTT                                                                            >  SRR4302540.1381153/1‑94 (MQ=60)
                    ATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTT                                                                            <  SRR4302540.1519690/94‑1 (MQ=60)
                      CCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCT                                                                          >  SRR4302540.8680253/1‑94 (MQ=60)
                      CCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCT                                                                          <  SRR4302540.4875038/94‑1 (MQ=60)
                      CCGCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCT                                                                          <  SRR4302540.4875311/94‑1 (MQ=60)
                       CTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTGTTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTG                                                                         >  SRR4302540.10057680/1‑94 (MQ=60)
                       CTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTG                                                                         >  SRR4302540.7101771/1‑94 (MQ=60)
                       CTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTG                                                                         >  SRR4302540.4094358/1‑94 (MQ=60)
                        TCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGC                                                                        <  SRR4302540.2111623/94‑1 (MQ=60)
                         CGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCC                                                                       >  SRR4302540.5362266/1‑94 (MQ=60)
                          GAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCT                                                                      >  SRR4302540.4996764/1‑94 (MQ=60)
                          GAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCT                                                                      >  SRR4302540.8688665/1‑94 (MQ=60)
                           AAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTT                                                                     >  SRR4302540.8611314/1‑94 (MQ=60)
                            AGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTT                                                                    >  SRR4302540.7081905/1‑94 (MQ=60)
                            AGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTT                                                                    >  SRR4302540.3388962/1‑94 (MQ=60)
                            AGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTT                                                                    <  SRR4302540.6841511/94‑1 (MQ=60)
                             GCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATGGCCACGGGCGTGGATGACTTTTCTGCCTTTG                                                                   >  SRR4302540.8782805/1‑94 (MQ=60)
                             GCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTG                                                                   <  SRR4302540.8893161/94‑1 (MQ=60)
                             GCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTG                                                                   <  SRR4302540.3541451/94‑1 (MQ=60)
                               CGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGTCACGGGCGTGGCTGACTGTTCTGCCTTTGgt                                                                 >  SRR4302540.1374752/1‑92 (MQ=60)
                               CGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCG                                                                 >  SRR4302540.5384840/1‑94 (MQ=60)
                               CGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCG                                                                 <  SRR4302540.3014227/94‑1 (MQ=60)
                               CGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCG                                                                 >  SRR4302540.6604771/1‑94 (MQ=60)
                                GTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGT                                                                >  SRR4302540.10132706/1‑94 (MQ=60)
                                  TTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCG                                                              >  SRR4302540.174396/1‑94 (MQ=60)
                                  TTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCG                                                              >  SRR4302540.7605085/1‑94 (MQ=60)
                                  TTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCG                                                              >  SRR4302540.7605084/1‑94 (MQ=60)
                                  TTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCG                                                              <  SRR4302540.5797664/94‑1 (MQ=60)
                                   TGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGG                                                             >  SRR4302540.7118227/1‑94 (MQ=60)
                                   TGGGGATTTTTCTCCGCCTATGCATTGGTGTCTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGG                                                             <  SRR4302540.9214083/94‑1 (MQ=60)
                                    GGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGT                                                            <  SRR4302540.4949634/94‑1 (MQ=60)
                                     GGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTT                                                           >  SRR4302540.4448171/1‑94 (MQ=60)
                                      GGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTG                                                          >  SRR4302540.8406772/1‑94 (MQ=60)
                                       GATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGT                                                         >  SRR4302540.5335617/1‑94 (MQ=60)
                                       GATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGT                                                         <  SRR4302540.315110/94‑1 (MQ=60)
                                       GATTTTTCTCCGCCTATGCATTGGTGTTTAGTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGT                                                         <  SRR4302540.6146749/94‑1 (MQ=60)
                                        ATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTT                                                        >  SRR4302540.2768497/1‑94 (MQ=60)
                                        ATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTT                                                        >  SRR4302540.7448032/1‑94 (MQ=60)
                                        ATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTT                                                        >  SRR4302540.9484666/1‑94 (MQ=60)
                                         TTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTG                                                       >  SRR4302540.5267758/1‑94 (MQ=60)
                                         TTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTG                                                       >  SRR4302540.4250043/1‑94 (MQ=60)
                                          TTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGC                                                      >  SRR4302540.4136418/1‑94 (MQ=60)
                                          TTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGC                                                      >  SRR4302540.8251415/1‑94 (MQ=60)
                                            TTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGA                                                    >  SRR4302540.1721715/1‑94 (MQ=60)
                                             TCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGAC                                                   <  SRR4302540.929433/94‑1 (MQ=60)
                                              CTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACA                                                  <  SRR4302540.6342134/94‑1 (MQ=60)
                                              CTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACA                                                  <  SRR4302540.6342412/94‑1 (MQ=60)
                                              CTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACA                                                  >  SRR4302540.5048577/1‑94 (MQ=60)
                                              CTCCGCCTATGCATTGGTGTGTATTGTTAGCTTGCTGGCGATTAGCGCCTCGGGCGTGGATGACTCGGCTGCCTTTGCGTCGGTTGTGGCGACT                                                  >  SRR4302540.7812814/1‑94 (MQ=25)
                                               TCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACAT                                                 <  SRR4302540.7433606/94‑1 (MQ=60)
                                               TCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACAT                                                 >  SRR4302540.7238163/1‑94 (MQ=60)
                                                CCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATT                                                >  SRR4302540.3962659/1‑94 (MQ=60)
                                                CCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATT                                                >  SRR4302540.5235867/1‑94 (MQ=60)
                                                CCGCCTATGCATTGGTGGTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGGCTTTGCGTCGGTTGTTGCGACATT                                                <  SRR4302540.3593779/94‑1 (MQ=60)
                                                 CGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTG                                               >  SRR4302540.477757/1‑94 (MQ=60)
                                                   CCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGCA                                             >  SRR4302540.2651804/1‑94 (MQ=60)
                                                   CCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAA                                             >  SRR4302540.3991142/1‑94 (MQ=60)
                                                   CCTATGCATGGGTGTTTATTGTCAGTATGCTGGCGAGTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAA                                             <  SRR4302540.6135106/94‑1 (MQ=60)
                                                    CTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAAT                                            <  SRR4302540.5111963/94‑1 (MQ=60)
                                                    CTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAAT                                            <  SRR4302540.6381532/94‑1 (MQ=60)
                                                     TATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATA                                           <  SRR4302540.8620418/94‑1 (MQ=60)
                                                     TATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATA                                           <  SRR4302540.3926305/94‑1 (MQ=60)
                                                     TATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATA                                           >  SRR4302540.1423418/1‑94 (MQ=60)
                                                     TATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATA                                           >  SRR4302540.1423417/1‑94 (MQ=60)
                                                      ATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAA                                          >  SRR4302540.8872130/1‑94 (MQ=60)
                                                      ATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAA                                          <  SRR4302540.3813692/94‑1 (MQ=60)
                                                      ATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAA                                          >  SRR4302540.2621578/1‑94 (MQ=60)
                                                       TGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAAC                                         <  SRR4302540.7358410/94‑1 (MQ=60)
                                                        GCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACC                                        >  SRR4302540.4288999/1‑94 (MQ=60)
                                                        GCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACC                                        <  SRR4302540.6538220/94‑1 (MQ=60)
                                                        GCATTGGTGTTTATGGTCAGTATGCTGTCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACC                                        <  SRR4302540.6538219/94‑1 (MQ=60)
                                                         CATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCT                                       >  SRR4302540.5487782/1‑94 (MQ=60)
                                                         CATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCT                                       >  SRR4302540.101336/1‑94 (MQ=60)
                                                         CATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCT                                       >  SRR4302540.844098/1‑94 (MQ=60)
                                                          ATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTG                                      >  SRR4302540.5138365/1‑94 (MQ=60)
                                                          ATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTG                                      <  SRR4302540.3617545/94‑1 (MQ=60)
                                                          ATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTG                                      >  SRR4302540.4388179/1‑94 (MQ=60)
                                                          ATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTG                                      >  SRR4302540.7530965/1‑94 (MQ=60)
                                                           TTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGG                                     >  SRR4302540.432422/1‑94 (MQ=60)
                                                           TTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGG                                     <  SRR4302540.8499365/94‑1 (MQ=60)
                                                           TTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGG                                     <  SRR4302540.8499374/94‑1 (MQ=60)
                                                           TTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGG                                     >  SRR4302540.8046348/1‑94 (MQ=60)
                                                           TTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGG                                     <  SRR4302540.2838166/94‑1 (MQ=60)
                                                            TGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGG                                    >  SRR4302540.6551786/1‑94 (MQ=60)
                                                            TGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGG                                    <  SRR4302540.5458943/94‑1 (MQ=60)
                                                            TGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGG                                    >  SRR4302540.7780642/1‑94 (MQ=60)
                                                            TGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGGTGTTGCGGCGTTGAATAACCTGGG                                    >  SRR4302540.2319986/1‑94 (MQ=60)
                                                             GGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGG                                   >  SRR4302540.5768950/1‑94 (MQ=60)
                                                             GGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGG                                   <  SRR4302540.9692622/94‑1 (MQ=60)
                                                             GGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGG                                   <  SRR4302540.3702361/94‑1 (MQ=60)
                                                             GGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGG                                   <  SRR4302540.6491233/94‑1 (MQ=60)
                                                              GTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGC                                  >  SRR4302540.8591234/1‑94 (MQ=60)
                                                              GTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGC                                  >  SRR4302540.1756278/1‑94 (MQ=60)
                                                              GTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGC                                  >  SRR4302540.3767158/1‑94 (MQ=60)
                                                              GTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGC                                  >  SRR4302540.737379/1‑94 (MQ=60)
                                                              GTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGC                                  >  SRR4302540.1724721/1‑94 (MQ=60)
                                                              GTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGC                                  >  SRR4302540.7007166/1‑94 (MQ=60)
                                                              GTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGC                                  >  SRR4302540.2485106/1‑94 (MQ=60)
                                                               TGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCC                                 >  SRR4302540.8082280/1‑94 (MQ=60)
                                                                GTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCA                                >  SRR4302540.7910115/1‑94 (MQ=60)
                                                                GTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCA                                >  SRR4302540.7582896/1‑94 (MQ=60)
                                                                GTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCA                                <  SRR4302540.2496986/94‑1 (MQ=60)
                                                                  TTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGG                              >  SRR4302540.7133724/1‑94 (MQ=60)
                                                                  TTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGG                              >  SRR4302540.3561056/1‑94 (MQ=60)
                                                                  TTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGG                              <  SRR4302540.4282576/94‑1 (MQ=60)
                                                                  TTATTGTCAGTATGCGGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGG                              <  SRR4302540.2094694/94‑1 (MQ=60)
                                                                    ATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGC                            >  SRR4302540.9119729/1‑94 (MQ=60)
                                                                    ATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGC                            >  SRR4302540.1363872/1‑94 (MQ=60)
                                                                     TTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCT                           >  SRR4302540.9445034/1‑94 (MQ=60)
                                                                     TTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCT                           >  SRR4302540.9631992/1‑94 (MQ=60)
                                                                     TTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCT                           >  SRR4302540.6913710/1‑94 (MQ=60)
                                                                      TGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTT                          >  SRR4302540.8671984/1‑94 (MQ=60)
                                                                      TGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTT                          >  SRR4302540.4511896/1‑94 (MQ=60)
                                                                       GTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTG                         >  SRR4302540.8408910/1‑94 (MQ=60)
                                                                       GTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTG                         <  SRR4302540.5140348/94‑1 (MQ=60)
                                                                        TCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGG                        <  SRR4302540.2404065/94‑1 (MQ=60)
                                                                        TCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATCGAATAACCTGGGGCCAGGGCTTGG                        <  SRR4302540.1513933/94‑1 (MQ=60)
                                                                          AGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCG                      >  SRR4302540.6620573/1‑94 (MQ=60)
                                                                          AGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCG                      >  SRR4302540.8490302/1‑94 (MQ=60)
                                                                          AGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCG                      <  SRR4302540.5203877/94‑1 (MQ=60)
                                                                           GTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTAGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGG                     >  SRR4302540.4706998/1‑94 (MQ=60)
                                                                            TATGCTGGCGATTATCGCCACGGGCGTGGCTGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGTGGACAGGGCTTGGCGTG                    >  SRR4302540.9626744/1‑94 (MQ=60)
                                                                            TATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTG                    <  SRR4302540.4514955/94‑1 (MQ=60)
                                                                            TATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTG                    >  SRR4302540.9626745/1‑94 (MQ=60)
                                                                            TATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTG                    <  SRR4302540.3954075/94‑1 (MQ=60)
                                                                             ATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGG                   <  SRR4302540.8845290/94‑1 (MQ=60)
                                                                             ATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGG                   >  SRR4302540.10088832/1‑94 (MQ=60)
                                                                             ATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGG                   >  SRR4302540.4670547/1‑94 (MQ=60)
                                                                             ATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGG                   >  SRR4302540.4355379/1‑94 (MQ=60)
                                                                               GCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTT                 >  SRR4302540.9238393/1‑94 (MQ=60)
                                                                                CTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTG                >  SRR4302540.8340473/1‑94 (MQ=60)
                                                                                CTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTG                >  SRR4302540.363431/1‑94 (MQ=60)
                                                                                CTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTG                <  SRR4302540.7104848/94‑1 (MQ=60)
                                                                                 TGGCGATTATCGCCACGGGCGTGGATTACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGC               <  SRR4302540.7578735/94‑1 (MQ=60)
                                                                                 TGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGC               >  SRR4302540.5301091/1‑94 (MQ=60)
                                                                                  GGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCT              <  SRR4302540.9462286/94‑1 (MQ=60)
                                                                                  GGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCT              >  SRR4302540.87947/1‑94 (MQ=60)
                                                                                   GCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTG             >  SRR4302540.3611853/1‑94 (MQ=60)
                                                                                    CGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGA            >  SRR4302540.380724/1‑94 (MQ=60)
                                                                                    CGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGA            >  SRR4302540.3483497/1‑94 (MQ=60)
                                                                                    CGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGA            >  SRR4302540.3402655/1‑94 (MQ=60)
                                                                                     GATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGAT           >  SRR4302540.4487724/1‑94 (MQ=60)
                                                                                     GATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGAT           >  SRR4302540.7770245/1‑94 (MQ=60)
                                                                                     GATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGAT           >  SRR4302540.3626593/1‑94 (MQ=60)
                                                                                     GATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTCGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGAT           >  SRR4302540.8434388/1‑94 (MQ=60)
                                                                                       TTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAA         >  SRR4302540.915259/1‑94 (MQ=60)
                                                                                        TATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAAT        >  SRR4302540.323402/1‑94 (MQ=60)
                                                                                        TATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAAC        <  SRR4302540.1480397/94‑1 (MQ=60)
                                                                                         ATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACT       <  SRR4302540.669464/94‑1 (MQ=60)
                                                                                          TCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGGCGGTTGTTGCGACATTGAATAACCTGGGGCCCGGGCTTGGCGTGGTTGCgcagaaatt      >  SRR4302540.2226575/1‑85 (MQ=60)
                                                                                           cgcCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACTTT     >  SRR4302540.8346962/4‑94 (MQ=60)
                                                                                           cgcCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCGTGGCGTGGGTGCTGAGAACTTT     >  SRR4302540.9099336/4‑94 (MQ=60)
                                                                                            gcCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTTGCGTGGTTGCTGATAACTTTA    >  SRR4302540.4594942/3‑94 (MQ=60)
                                                                                            gcCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATCACTTTA    >  SRR4302540.3733851/3‑94 (MQ=60)
                                                                                            gcCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACTTTA    >  SRR4302540.468684/3‑94 (MQ=60)
                                                                                            gcCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGCCCAGGGCTggcctggttgccggaaaactttc    >  SRR4302540.1701798/3‑71 (MQ=60)
                                                                                            C‑CACGGGCGTGGATGTCTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACTTTAC   <  SRR4302540.2353695/94‑1 (MQ=60)
                                                                                            C‑CACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACTTTAC   <  SRR4302540.4694462/94‑1 (MQ=60)
                                                                                              CACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACTTTACC  >  SRR4302540.530771/1‑94 (MQ=60)
                                                                                              |                                                                                             
ATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑CACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACTTTACC  >  NC_000913/4031891‑4032077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑