Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,067,548 T→C H137R (CAC→CGC)  b3879 ← putative aldose‑1‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,067,5480TC99.6% 753.8 / ‑4.4 234H137R (CAC→CGC) b3879putative aldose‑1‑epimerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (116/117);  minor base A (0/1);  total (116/118)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.35e-01

Only 100 of 266 total aligned reads displayed.

TTGCAGGTTAAATAAGGATGAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGTGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGGGTAACGCTGGTG  >  NC_000913/4067458‑4067637
                                                                                          |                                                                                         
 tGCAGGTTAAATAAGGATGAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCcgtgcgtg                                                                                        <  1:5812906/93‑1 (MQ=255)
 tGCAGGTTAAATAAGGATGAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCcgtgcgtg                                                                                        <  2:1626795/93‑1 (MQ=255)
 tGCAGGTTAAATAAGGATGAATACCCACGCCATAAGGCGCTGCGACACTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCcgtgcgtg                                                                                        <  1:4085148/93‑1 (MQ=255)
  gCAGGTAAAATAAGAATGAATCCCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCgtgcgtgc                                                                                       <  2:5422244/93‑1 (MQ=255)
  gCAGATTAAATAAAGATGAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCgtgcgtgc                                                                                       <  1:4469907/93‑1 (MQ=255)
   cAGGTTAAATAAGGATGAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGtgcgtgcg                                                                                      >  2:3815597/1‑93 (MQ=255)
      gTTAAATAAGGATGAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTg                                                                                   >  2:4225456/1‑93 (MQ=255)
      gTTAAATAAGGATGAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTg                                                                                   >  1:457417/1‑93 (MQ=255)
       ttAAATAAGGATGAATACCCACGCCATAAGGCGCTGCGGCAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGa                                                                                  <  1:2871499/93‑1 (MQ=255)
        tAAATAAGGATGAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAg                                                                                 >  1:5477117/1‑93 (MQ=255)
         aaaTAAGGATGAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGc                                                                                >  2:4859302/1‑93 (MQ=255)
            tAAGGATGAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGaa                                                                             >  2:1155220/1‑93 (MQ=255)
            tAAGGATGAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGaa                                                                             >  1:4639026/1‑93 (MQ=255)
             aaGGATGAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAAt                                                                            <  2:4500918/93‑1 (MQ=255)
               ggATGAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATaa                                                                          >  1:6094176/1‑93 (MQ=255)
               ggATGAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATaa                                                                          >  1:658877/1‑93 (MQ=255)
               ggATGAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATaa                                                                          >  2:4080442/1‑93 (MQ=255)
                gATGAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATaaa                                                                         >  1:3278857/1‑93 (MQ=255)
                  tGAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAaaca                                                                       <  1:4256861/93‑1 (MQ=255)
                   gAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAaacaa                                                                      <  2:2634201/93‑1 (MQ=255)
                   gAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAaacaa                                                                      >  1:3423194/1‑93 (MQ=255)
                    aaTACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAaacaac                                                                     >  1:4945864/1‑93 (MQ=255)
                     aTACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACt                                                                    <  1:3464621/93‑1 (MQ=255)
                     aTACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACt                                                                    <  2:1527558/93‑1 (MQ=255)
                       aCCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTtg                                                                  <  2:3497133/93‑1 (MQ=255)
                       aCCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTtg                                                                  >  1:1847675/1‑93 (MQ=255)
                        cccACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTtgt                                                                 <  1:256508/93‑1 (MQ=255)
                        cccACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTtgt                                                                 <  2:4013210/93‑1 (MQ=255)
                        cccACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGCGCGTGCGTTGAGCGAATAAACAACTtgt                                                                 >  1:1366726/1‑93 (MQ=255)
                          cACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGa                                                               >  1:1665742/1‑93 (MQ=255)
                           aCGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGaa                                                              <  2:2499190/93‑1 (MQ=255)
                           aCGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGaa                                                              <  1:869245/93‑1 (MQ=255)
                            cGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAg                                                             <  2:4571891/93‑1 (MQ=255)
                             gCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGc                                                            <  1:1545275/93‑1 (MQ=255)
                              ccATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGcc                                                           >  1:5030657/1‑93 (MQ=255)
                               cATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCa                                                          >  1:4207638/1‑93 (MQ=255)
                               cATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCa                                                          >  1:3802843/1‑93 (MQ=255)
                               cATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCa                                                          <  2:275907/93‑1 (MQ=255)
                               cATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCa                                                          <  1:5205979/93‑1 (MQ=255)
                                  aaGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCa                                                       <  2:222548/93‑1 (MQ=255)
                                     gcgcTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATaa                                                    <  1:1234580/93‑1 (MQ=255)
                                     gcgcTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATaa                                                    <  2:5255394/93‑1 (MQ=255)
                                      cgcTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATaaa                                                   <  2:4575892/93‑1 (MQ=255)
                                      cgcTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATaaa                                                   <  1:5998462/93‑1 (MQ=255)
                                        cTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTTCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACg                                                 >  1:3612496/1‑93 (MQ=255)
                                         tGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACgg                                                >  2:883042/1‑93 (MQ=255)
                                         tGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACgg                                                >  1:1753535/1‑93 (MQ=255)
                                         tGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACgg                                                <  1:5421907/93‑1 (MQ=255)
                                          gCGACAGTGCAGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGTGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGa                                               <  1:4335478/93‑1 (MQ=255)
                                           cGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGAt                                              <  1:5576947/93‑1 (MQ=255)
                                           cGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGAt                                              <  1:4707761/93‑1 (MQ=255)
                                           cGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGAt                                              <  1:2312599/93‑1 (MQ=255)
                                            gACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATa                                             <  2:440216/93‑1 (MQ=255)
                                            gACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATa                                             <  2:2958132/93‑1 (MQ=255)
                                             aCAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAg                                            <  2:3367199/93‑1 (MQ=255)
                                             aCAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAg                                            >  2:6292501/1‑93 (MQ=255)
                                             aCAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAg                                            >  2:202272/1‑93 (MQ=255)
                                               aGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGcc                                          >  1:2043404/1‑93 (MQ=255)
                                                gTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCg                                         >  1:1969668/1‑93 (MQ=255)
                                                 tGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGt                                        >  1:5871884/1‑93 (MQ=255)
                                                 tGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGt                                        <  2:1572530/93‑1 (MQ=255)
                                                  gCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACCGATAGCCgca                                       >  2:5224180/1‑91 (MQ=255)
                                                    cGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAAc                                     <  2:5619220/93‑1 (MQ=255)
                                                       tatTCTGGCTTTCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTgg                                  <  2:1871789/93‑1 (MQ=255)
                                                       tatTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTgg                                  <  2:1746537/93‑1 (MQ=255)
                                                       tatTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTgg                                  >  2:6308247/1‑93 (MQ=255)
                                                       tatTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTgg                                  <  2:1199234/93‑1 (MQ=255)
                                                        atTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTggg                                 >  1:130846/1‑93 (MQ=255)
                                                         ttCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGggc                                >  1:4861171/1‑93 (MQ=255)
                                                            tGGCTTGCAATCTCAACGGTAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGggcggc                             >  2:6119471/1‑93 (MQ=255)
                                                              gCTTGCGATCTCAACGGAAAGACCCGTGCGGGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCCGCAg                           >  2:3290476/1‑93 (MQ=255)
                                                              gCTTGCGATCTCAACGGAAAGACCCGTGAGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAg                           <  2:5884714/93‑1 (MQ=255)
                                                                ttGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGCATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGaa                         >  1:6226903/1‑93 (MQ=255)
                                                                 tGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGaaa                        >  2:5673500/1‑93 (MQ=255)
                                                                  gCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGaaaa                       <  1:3091302/93‑1 (MQ=255)
                                                                  gCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGaaaa                       >  2:1347244/1‑93 (MQ=255)
                                                                    gATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAGCGGATAGCCGTAACTGGGCGGCAGAAAAGc                     <  1:1010432/93‑1 (MQ=255)
                                                                     aTCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCt                    <  1:2649182/93‑1 (MQ=255)
                                                                     aTCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCt                    <  2:5099459/93‑1 (MQ=255)
                                                                       ctcAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGt                  <  2:779614/93‑1 (MQ=255)
                                                                        tcAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTc                 >  1:2467869/1‑93 (MQ=255)
                                                                        tcAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTc                 <  1:4481468/93‑1 (MQ=255)
                                                                        tcAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTc                 >  2:5524861/1‑93 (MQ=255)
                                                                           aCGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAgg              >  1:276585/1‑93 (MQ=255)
                                                                           aCGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAgg              >  1:1113317/1‑93 (MQ=255)
                                                                            cGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAggg             >  2:180880/1‑93 (MQ=255)
                                                                             ggAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAggga            >  1:1210632/1‑92 (MQ=255)
                                                                             ggAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGGGt            >  1:377191/1‑93 (MQ=255)
                                                                              gAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGGGTa           >  1:250897/1‑93 (MQ=255)
                                                                              gAAAGACCC‑TGGCTGCCTTTAGCCAATAAACCACTTGGGGAACCCGGAAAAACCGAATGGCGTAACTGGGCGGCAGAAAAGCTGTCAGGGTaa          <  2:4158641/93‑1 (MQ=255)
                                                                               aaaGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGGGTaa          <  2:598641/93‑1 (MQ=255)
                                                                               aaaGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGGGTaa          <  1:5755259/93‑1 (MQ=255)
                                                                                aaGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGGGTAAc         <  2:4308102/93‑1 (MQ=255)
                                                                                  gACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGGGTAACGc       >  2:3167234/1‑93 (MQ=255)
                                                                                  gACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGGGTAACGc       <  2:138653/93‑1 (MQ=255)
                                                                                    cccGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGGGTAACGCtg     >  1:1401491/1‑93 (MQ=255)
                                                                                     ccGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGGGTAACGCtgg    <  2:3558049/93‑1 (MQ=255)
                                                                                     ccGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGGGTAACGCtgg    >  1:2912863/1‑93 (MQ=255)
                                                                                      cgtgcgtgCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGGGTAACGCtggt   <  2:2095880/93‑1 (MQ=255)
                                                                                       gtgcgtgcGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGGGTAACGCtggtg  <  2:5084865/93‑1 (MQ=255)
                                                                                          |                                                                                         
TTGCAGGTTAAATAAGGATGAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGTGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGGGTAACGCTGGTG  >  NC_000913/4067458‑4067637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAGTTGCAGGTTAAATAAGGATGAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGTGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGGGTAACGCTGGTGGCGG  >  NC_000913/4067455‑4067641
                                                                                             |                                                                                             
AAGTTGCAGGTTAAATAAGGATGAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGC                                                                                               >  SRR4302533.3490691/1‑94 (MQ=60)
 AGTTGCAGGTTAAATAAGGATGAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCG                                                                                              >  SRR4302533.1650064/1‑94 (MQ=60)
   GTGCAGGTTAAATAAGGATGAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTG                                                                                            <  SRR4302533.2487914/94‑1 (MQ=60)
    TGCAGGTTAAATAAGGATGAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGC                                                                                           <  SRR4302533.5848014/94‑1 (MQ=60)
    TGCAGGTTAAATAAGGATGAATACCCACGCCATAAGGCGCTGCGACACTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGC                                                                                           <  SRR4302533.4110016/94‑1 (MQ=60)
     GCAGGTTAAATAAGGATGAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCG                                                                                          <  SRR4302533.6303350/94‑1 (MQ=60)
     GCAGATTAAATAAAGATGAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCG                                                                                          <  SRR4302533.4496941/94‑1 (MQ=60)
      CAGGTTAAATAAGGATGAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGT                                                                                         <  SRR4302533.1837094/94‑1 (MQ=60)
       AGGTTAAATAAGGATGAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTT                                                                                        <  SRR4302533.115951/94‑1 (MQ=60)
       AGGTTAAATAAGGATGAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTT                                                                                        <  SRR4302533.902851/94‑1 (MQ=60)
       AGGTTAAATAAGGATGAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTT                                                                                        >  SRR4302533.2871224/1‑94 (MQ=60)
        GGTTAAATAAGGATGAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTG                                                                                       >  SRR4302533.2892499/1‑94 (MQ=60)
        GGTTAAATAAGGATGAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTG                                                                                       >  SRR4302533.460153/1‑94 (MQ=60)
          TTAAATAAGGATGAATACCCACGCCATAAGGCGCTGCGGCAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAG                                                                                     <  SRR4302533.2889167/94‑1 (MQ=60)
          TTAAATAAGGATGAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAG                                                                                     >  SRR4302533.5510130/1‑94 (MQ=60)
          TTAAATAAGGATGAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAG                                                                                     <  SRR4302533.1584419/94‑1 (MQ=60)
           TAAATAAGGATGAATACCCACGCCATAAGGCGCTGCGACAATGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGC                                                                                    >  SRR4302533.4580855/1‑94 (MQ=60)
            AAATAAGGATGAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCG                                                                                   >  SRR4302533.42724/1‑94 (MQ=60)
            AAATAAGGATGAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCG                                                                                   <  SRR4302533.1429693/94‑1 (MQ=60)
             AATAAGGATGAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGAACCGTGCGTGCGTTGCGCGA                                                                                  >  SRR4302533.3699631/1‑94 (MQ=60)
              ATAAGGATGAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAA                                                                                 >  SRR4302533.2627020/1‑94 (MQ=60)
              ATAAGGATGAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAA                                                                                 >  SRR4302533.4667136/1‑94 (MQ=60)
                 AGGATGAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAA                                                                              >  SRR4302533.662759/1‑94 (MQ=60)
                 AGGATGAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAA                                                                              <  SRR4302533.4828099/94‑1 (MQ=60)
                 AGGATGAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAA                                                                              >  SRR4302533.6130996/1‑94 (MQ=60)
                  GGATGAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAA                                                                             >  SRR4302533.3299102/1‑94 (MQ=60)
                     TGAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAA                                                                          <  SRR4302533.4282526/94‑1 (MQ=60)
                     TGAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAA                                                                          >  SRR4302533.3444339/1‑94 (MQ=60)
                     TGAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAA                                                                          >  SRR4302533.2565286/1‑94 (MQ=60)
                      GAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAAC                                                                         >  SRR4302533.4975798/1‑94 (MQ=60)
                      GAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAAC                                                                         <  SRR4302533.6143947/94‑1 (MQ=60)
                        ATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTT                                                                       <  SRR4302533.3486007/94‑1 (MQ=60)
                         TACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTG                                                                      >  SRR4302533.1858943/1‑94 (MQ=60)
                         TACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTG                                                                      <  SRR4302533.3581061/94‑1 (MQ=60)
                         TACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTG                                                                      <  SRR4302533.5712007/94‑1 (MQ=60)
                          ACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGCGCGTGCGTTGAGCGAATAAACAACTTGT                                                                     >  SRR4302533.1374941/1‑94 (MQ=60)
                           CCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTG                                                                    <  SRR4302533.258073/94‑1 (MQ=60)
                            CCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGA                                                                   >  SRR4302533.1675808/1‑94 (MQ=60)
                             CACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAA                                                                  <  SRR4302533.6257364/94‑1 (MQ=60)
                             CACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAA                                                                  <  SRR4302533.4334661/94‑1 (MQ=60)
                             CACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAA                                                                  <  SRR4302533.4666640/94‑1 (MQ=60)
                              ACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAG                                                                 <  SRR4302533.2856738/94‑1 (MQ=60)
                              ACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAG                                                                 <  SRR4302533.4472292/94‑1 (MQ=60)
                              ACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAG                                                                 <  SRR4302533.2919765/94‑1 (MQ=60)
                              ACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAG                                                                 <  SRR4302533.874347/94‑1 (MQ=60)
                                GCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCC                                                               >  SRR4302533.2029824/1‑94 (MQ=60)
                                GCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCC                                                               >  SRR4302533.5061117/1‑94 (MQ=60)
                                GCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCC                                                               <  SRR4302533.1554585/94‑1 (MQ=60)
                                 CCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCA                                                              >  SRR4302533.3826112/1‑94 (MQ=60)
                                 CCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCA                                                              >  SRR4302533.244216/1‑94 (MQ=60)
                                 CCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCA                                                              >  SRR4302533.4233093/1‑94 (MQ=60)
                                 CCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCA                                                              >  SRR4302533.4005865/1‑94 (MQ=60)
                                  CATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAG                                                             >  SRR4302533.5696933/1‑94 (MQ=60)
                                  CATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAG                                                             <  SRR4302533.5237347/94‑1 (MQ=60)
                                   ATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGC                                                            >  SRR4302533.2105771/1‑94 (MQ=60)
                                   ATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGC                                                            <  SRR4302533.5597632/94‑1 (MQ=60)
                                   ATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGC                                                            >  SRR4302533.4924053/1‑94 (MQ=60)
                                    TAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCA                                                           <  SRR4302533.4511797/94‑1 (MQ=60)
                                     AAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGATCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCAT                                                          <  SRR4302533.4464726/94‑1 (MQ=60)
                                     AAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCAT                                                          >  SRR4302533.1796921/1‑94 (MQ=60)
                                     AAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCAT                                                          >  SRR4302533.338012/1‑94 (MQ=60)
                                     AAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCAT                                                          <  SRR4302533.5961436/94‑1 (MQ=60)
                                     AAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCAT                                                          <  SRR4302533.5874334/94‑1 (MQ=60)
                                     AAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGCGAAGCCAGCAA                                                          >  SRR4302533.1227557/1‑94 (MQ=60)
                                     AAGGCGCGGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCAT                                                          <  SRR4302533.3149812/94‑1 (MQ=60)
                                      AGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATA                                                         >  SRR4302533.4497329/1‑94 (MQ=60)
                                       GGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAA                                                        <  SRR4302533.2689360/94‑1 (MQ=60)
                                        GCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAA                                                       <  SRR4302533.1241961/94‑1 (MQ=60)
                                         CGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAAC                                                      <  SRR4302533.6034793/94‑1 (MQ=60)
                                          GCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTTCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACG                                                     >  SRR4302533.3634729/1‑94 (MQ=60)
                                          GCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACG                                                     <  SRR4302533.2247063/94‑1 (MQ=60)
                                           CTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGG                                                    >  SRR4302533.1764218/1‑94 (MQ=60)
                                            TGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGA                                                   >  SRR4302533.133768/1‑94 (MQ=60)
                                            TGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGA                                                   <  SRR4302533.5454532/94‑1 (MQ=60)
                                             GCGACAGTGCAGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGTGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGAT                                                  <  SRR4302533.4361602/94‑1 (MQ=60)
                                              CGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATA                                                 <  SRR4302533.4736296/94‑1 (MQ=60)
                                              CGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATA                                                 <  SRR4302533.4945130/94‑1 (MQ=60)
                                              CGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATA                                                 <  SRR4302533.5610652/94‑1 (MQ=60)
                                              CGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATA                                                 <  SRR4302533.2326925/94‑1 (MQ=60)
                                                 CAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCC                                              >  SRR4302533.2056003/1‑94 (MQ=60)
                                                  AGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCG                                             >  SRR4302533.3049979/1‑94 (MQ=60)
                                                  AGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCG                                             <  SRR4302533.5051324/94‑1 (MQ=60)
                                                  AGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCG                                             >  SRR4302533.1981714/1‑94 (MQ=60)
                                                   GTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGT                                            >  SRR4302533.5907378/1‑94 (MQ=60)
                                                      CCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAAC                                         >  SRR4302533.1306594/1‑94 (MQ=60)
                                                      CCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAAC                                         >  SRR4302533.5348596/1‑94 (MQ=60)
                                                       CGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACT                                        <  SRR4302533.6116980/94‑1 (MQ=60)
                                                        GATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTG                                       >  SRR4302533.52454/1‑94 (MQ=60)
                                                        GATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTG                                       >  SRR4302533.5890446/1‑94 (MQ=60)
                                                         ATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGG                                      >  SRR4302533.1073276/1‑94 (MQ=60)
                                                         ATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGG                                      >  SRR4302533.3475057/1‑94 (MQ=60)
                                                          TATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGG                                     >  SRR4302533.131666/1‑94 (MQ=60)
                                                          TATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGG                                     <  SRR4302533.17329/94‑1 (MQ=60)
                                                           ATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGC                                    >  SRR4302533.4890632/1‑94 (MQ=60)
                                                                GGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAG                               <  SRR4302533.1356202/94‑1 (MQ=60)
                                                                GGCTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAG                               <  SRR4302533.2351713/94‑1 (MQ=60)
                                                                  CTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGCATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAA                             >  SRR4302533.6264206/1‑94 (MQ=60)
                                                                  CTTGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAA                             >  SRR4302533.3657891/1‑94 (MQ=60)
                                                                    TGCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAA                           <  SRR4302533.4183292/94‑1 (MQ=60)
                                                                     GCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAG                          <  SRR4302533.769910/94‑1 (MQ=60)
                                                                     GCGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAG                          <  SRR4302533.3110311/94‑1 (MQ=60)
                                                                      CGATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGC                         >  SRR4302533.5810503/1‑94 (MQ=60)
                                                                       GATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAGCGGATAGCCGTAACTGGGCGGCAGAAAAGCT                        <  SRR4302533.1016341/94‑1 (MQ=60)
                                                                        ATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTG                       <  SRR4302533.2665456/94‑1 (MQ=60)
                                                                        ATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTG                       >  SRR4302533.159815/1‑94 (MQ=60)
                                                                        ATCTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTG                       >  SRR4302533.4619985/1‑94 (MQ=60)
                                                                          CTCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTC                     >  SRR4302533.2483089/1‑94 (MQ=60)
                                                                           TCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCA                    <  SRR4302533.993348/94‑1 (MQ=60)
                                                                           TCAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCA                    <  SRR4302533.4508578/94‑1 (MQ=60)
                                                                            CAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAG                   <  SRR4302533.2574166/94‑1 (MQ=60)
                                                                            CAACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAG                   <  SRR4302533.3291536/94‑1 (MQ=60)
                                                                             AACGGAAAGACGCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGG                  <  SRR4302533.4787594/94‑1 (MQ=60)
                                                                             AACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGG                  >  SRR4302533.1119920/1‑94 (MQ=60)
                                                                             AACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGG                  >  SRR4302533.278269/1‑94 (MQ=60)
                                                                             AACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGG                  <  SRR4302533.5510794/94‑1 (MQ=60)
                                                                              ACGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGGG                 >  SRR4302533.3808964/1‑94 (MQ=60)
                                                                               CGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGGGT                >  SRR4302533.4720780/1‑94 (MQ=60)
                                                                               CGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGGGT                >  SRR4302533.5919186/1‑94 (MQ=60)
                                                                               CGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGGGT                >  SRR4302533.379464/1‑94 (MQ=60)
                                                                               CGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGGGT                >  SRR4302533.4072392/1‑94 (MQ=60)
                                                                               CGGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGGGA                >  SRR4302533.1217900/1‑94 (MQ=60)
                                                                                GGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGGGTA               <  SRR4302533.1541896/94‑1 (MQ=60)
                                                                                GGAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGGGTA               >  SRR4302533.252444/1‑94 (MQ=60)
                                                                                 GAAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGAGGCAGAAAAGCTGTCAGGGTAA              >  SRR4302533.1403921/1‑94 (MQ=60)
                                                                                  AAAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGGGTAAC             <  SRR4302533.5789985/94‑1 (MQ=60)
                                                                                   AAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGGGTAACG            <  SRR4302533.1191191/94‑1 (MQ=60)
                                                                                   AAGACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGGGTAACG            >  SRR4302533.3594437/1‑94 (MQ=60)
                                                                                      GCCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGGGTAACGCTG         <  SRR4302533.5186338/94‑1 (MQ=60)
                                                                                      ACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGGGTAACGCTG         <  SRR4302533.1246482/94‑1 (MQ=60)
                                                                                      ACCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGGGTAACGCTG         >  SRR4302533.1409941/1‑94 (MQ=60)
                                                                                       CCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGGGTAACGCTGG        <  SRR4302533.4117744/94‑1 (MQ=60)
                                                                                       CCCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGGGTAACGCTGG        >  SRR4302533.2930738/1‑94 (MQ=60)
                                                                                        CCGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGGGTAACGCTGGT       >  SRR4302533.5070277/1‑94 (MQ=60)
                                                                                         CGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGGGTAACGCTGGTG      >  SRR4302533.2599304/1‑94 (MQ=60)
                                                                                         CGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGGGTAACGCTGGTG      >  SRR4302533.6292827/1‑94 (MQ=60)
                                                                                         CGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGGGTAACGCTGGTG      <  SRR4302533.5455628/94‑1 (MQ=60)
                                                                                         CGTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGCCGGCAGACAAGCTGTCAGGGAAACGCTGGTG      >  SRR4302533.1955846/1‑94 (MQ=60)
                                                                                          GTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGGGTAACGCTGGTGG     <  SRR4302533.1939734/94‑1 (MQ=60)
                                                                                          GTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGGGTAACGCTGGTGG     >  SRR4302533.6251399/1‑94 (MQ=60)
                                                                                          GTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGGGTAACGCTGGTGG     <  SRR4302533.4307421/94‑1 (MQ=60)
                                                                                          GTGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGGGTAACGCTGGTGG     <  SRR4302533.332032/94‑1 (MQ=60)
                                                                                           TGCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGGGTAACGCTGGTGGC    <  SRR4302533.4132097/94‑1 (MQ=60)
                                                                                            GCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGGGTAACGCTGGTGGCG   <  SRR4302533.5138385/94‑1 (MQ=60)
                                                                                            GCGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGGGTAACGCTGGTGGCG   <  SRR4302533.4132848/94‑1 (MQ=60)
                                                                                             CGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGGGTAACGCTGGTGGCGG  <  SRR4302533.6162048/94‑1 (MQ=60)
                                                                                             CGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGGGTAACGCTGGTGGCGG  <  SRR4302533.1936060/94‑1 (MQ=60)
                                                                                             CGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGGGTAACGCTGGTGGCGG  <  SRR4302533.2945928/94‑1 (MQ=60)
                                                                                             CGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGGGTAACGCTGGTGGCGG  <  SRR4302533.56679/94‑1 (MQ=60)
                                                                                             |                                                                                             
AAGTTGCAGGTTAAATAAGGATGAATACCCACGCCATAAGGCGCTGCGACAGTGCCGATATTCTGGCTTGCGATCTCAACGGAAAGACCCGTGTGTGCGTTGAGCGAATAAACAACTTGTGAAGCCAGCATAAACGGATAGCCGTAACTGGGCGGCAGAAAAGCTGTCAGGGTAACGCTGGTGGCGG  >  NC_000913/4067455‑4067641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑