Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,075,942 T→C V305A (GTG→GCG)  yiiD → putative acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,075,9420TC99.4% 560.6 / ‑5.7 178V305A (GTG→GCG) yiiDputative acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (42/135);  total (42/136)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 186 total aligned reads displayed.

TAAGCGGCGATCTCGACCGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGG  >  NC_000913/4075852‑4076031
                                                                                          |                                                                                         
 aaccggcgATCTCGACCCTCTGGCGCGCGGCCGACAAGCACGCGCGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGtt                                                                                        <  1:388663/90‑1 (MQ=255)
  aGCGGCGATCTCGACCGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGttt                                                                                       <  1:2924237/93‑1 (MQ=255)
  aGCGGCGATCTCGACCGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGttt                                                                                       <  1:1973369/93‑1 (MQ=255)
   gcggcgATCTCGACCGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGGGCAGCGTTTg                                                                                      >  1:5058245/1‑93 (MQ=255)
    cggcgATCTCGACCGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGa                                                                                     <  2:5561160/93‑1 (MQ=255)
    cggcgATCTCGACCGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGa                                                                                     <  1:3069515/93‑1 (MQ=255)
     ggcgATCTCGACCGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGaa                                                                                    <  1:1041211/93‑1 (MQ=255)
       cgATCTCGACCGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAgg                                                                                  >  2:961720/1‑93 (MQ=255)
        gATCTCGACCGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGc                                                                                 <  1:3855425/93‑1 (MQ=255)
        gATCTCGACCGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGc                                                                                 >  1:2827560/1‑93 (MQ=255)
         aTCTCGACCGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCa                                                                                >  1:3461894/1‑93 (MQ=255)
          tctcGACCGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCAc                                                                               >  1:548348/1‑93 (MQ=255)
          tctcGACCGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCAc                                                                               <  2:2166236/93‑1 (MQ=255)
           ctcGACCGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACg                                                                              >  2:2959081/1‑93 (MQ=255)
            tcGACCGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGt                                                                             <  2:6050864/93‑1 (MQ=255)
             cGACCGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGta                                                                            <  1:6137601/93‑1 (MQ=255)
             cGACCGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGta                                                                            <  2:309435/93‑1 (MQ=255)
              gACCGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGtat                                                                           >  2:3260885/1‑93 (MQ=255)
              gACCGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGtat                                                                           >  1:4919465/1‑93 (MQ=255)
               aCCGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGtata                                                                          >  2:5046414/1‑93 (MQ=255)
               aCCGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGtata                                                                          <  1:983677/93‑1 (MQ=255)
               aCCGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGtata                                                                          <  1:3523514/93‑1 (MQ=255)
                ccGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTatat                                                                         <  2:5676864/93‑1 (MQ=255)
                ccGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTatat                                                                         <  1:3298494/93‑1 (MQ=255)
                ccGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTatat                                                                         <  2:816078/93‑1 (MQ=255)
                 cGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATc                                                                        <  2:2312987/93‑1 (MQ=255)
                    cTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGtt                                                                     <  2:4961764/93‑1 (MQ=255)
                     tGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTTTATCGTTc                                                                    <  1:6009736/93‑1 (MQ=255)
                     tGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTc                                                                    <  2:2806563/93‑1 (MQ=255)
                     tGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTc                                                                    <  1:5222700/93‑1 (MQ=255)
                     tGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTc                                                                    <  1:4524315/93‑1 (MQ=255)
                     tGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTc                                                                    <  1:649530/93‑1 (MQ=255)
                      ggCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCt                                                                   <  2:6042468/93‑1 (MQ=255)
                       gcgcgcGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTg                                                                  <  1:2574505/93‑1 (MQ=255)
                        cgcgcgGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGc                                                                 <  1:1730212/93‑1 (MQ=255)
                         gcgcgGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCGGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGcc                                                                <  1:2364438/93‑1 (MQ=255)
                         gcgcgGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGcc                                                                <  1:430461/93‑1 (MQ=255)
                         gcgcgGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGcc                                                                <  1:5816099/93‑1 (MQ=255)
                          cgcgGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGccc                                                               <  1:3205377/93‑1 (MQ=255)
                              gACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGa                                                           <  2:6156996/93‑1 (MQ=255)
                              gACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGa                                                           <  1:5998613/93‑1 (MQ=255)
                               aCGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGaa                                                          >  1:4670724/1‑93 (MQ=255)
                               aCGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGaa                                                          <  1:5343935/93‑1 (MQ=255)
                                     aGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCAtt                                                    >  1:4824843/1‑93 (MQ=255)
                                      gCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCAttt                                                   <  2:4574053/93‑1 (MQ=255)
                                      gCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCAttt                                                   <  1:3344970/93‑1 (MQ=255)
                                       cACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTg                                                  >  2:1341607/1‑93 (MQ=255)
                                        aCGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTgg                                                 >  2:2413262/1‑93 (MQ=255)
                                        aCGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTgg                                                 >  2:333242/1‑93 (MQ=255)
                                           ggTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGccc                                              <  1:5768988/93‑1 (MQ=255)
                                           ggTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGccc                                              >  2:6237594/1‑93 (MQ=255)
                                            gTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCg                                             >  2:6033256/1‑93 (MQ=255)
                                              gCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCTCGTa                                           >  2:5164997/1‑93 (MQ=255)
                                              gCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTa                                           <  2:5903172/93‑1 (MQ=255)
                                              gCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTa                                           <  2:3275354/93‑1 (MQ=255)
                                               cAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTAt                                          <  2:1997430/93‑1 (MQ=255)
                                                aGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATg                                         <  1:4019139/93‑1 (MQ=255)
                                                aGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATg                                         >  2:755329/1‑93 (MQ=255)
                                                 gATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGa                                        <  1:4349695/93‑1 (MQ=255)
                                                    gCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAaga                                     <  2:1774469/93‑1 (MQ=255)
                                                    gCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAaga                                     <  2:936629/93‑1 (MQ=255)
                                                    gCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAaga                                     <  1:4782051/93‑1 (MQ=255)
                                                    gCAGGTCGAAATCGTTGGCGACGAGACGTCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAaga                                     <  1:2985221/93‑1 (MQ=255)
                                                     cAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAagag                                    <  2:2511622/93‑1 (MQ=255)
                                                       ggTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAggg                                  <  1:874449/93‑1 (MQ=255)
                                                       ggTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAggg                                  <  2:5369261/93‑1 (MQ=255)
                                                        gTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGc                                 <  1:1308377/93‑1 (MQ=255)
                                                        gTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGc                                 <  1:4587140/93‑1 (MQ=255)
                                                          cGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCgg                               <  1:2628713/93‑1 (MQ=255)
                                                            aaaTCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGa                             <  1:6190987/93‑1 (MQ=255)
                                                             aaTCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGaa                            <  2:1531398/93‑1 (MQ=255)
                                                             aaTCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGaa                            >  2:3823971/1‑93 (MQ=255)
                                                              aTCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAAc                           >  1:1158929/1‑93 (MQ=255)
                                                               tCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACg                          >  1:4248663/1‑93 (MQ=255)
                                                                cTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACga                         >  2:4761984/1‑93 (MQ=255)
                                                                 tttGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACgaa                        <  1:3942540/93‑1 (MQ=255)
                                                                 tgtGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACgaa                        >  1:6090790/3‑93 (MQ=255)
                                                                   tGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACgaaga                      >  2:5692922/1‑93 (MQ=255)
                                                                   tGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACgaaga                      <  1:2029399/93‑1 (MQ=255)
                                                                   tGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACgaaga                      <  2:4066710/93‑1 (MQ=255)
                                                                   tGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACgaaga                      <  2:2742102/93‑1 (MQ=255)
                                                                   tGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACgaaga                      <  1:225111/93‑1 (MQ=255)
                                                                   tGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACgaaga                      <  1:2847573/93‑1 (MQ=255)
                                                                     gCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCGCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGaagaag                    <  1:3736463/93‑1 (MQ=255)
                                                                       gacgaGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAagag                  <  2:2476268/93‑1 (MQ=255)
                                                                        acgaGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGt                 <  2:293941/93‑1 (MQ=255)
                                                                          gagaCGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAg               <  2:4624732/93‑1 (MQ=255)
                                                                           agaCGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGc              <  2:3616072/93‑1 (MQ=255)
                                                                           agaCGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGc              <  2:5285277/93‑1 (MQ=255)
                                                                            gaCGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCt             >  2:3458336/1‑93 (MQ=255)
                                                                            gaCGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCt             <  2:5401871/93‑1 (MQ=255)
                                                                            gaCGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCt             <  1:1959508/93‑1 (MQ=255)
                                                                               gCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGAt          <  1:1034810/93‑1 (MQ=255)
                                                                                gcGGGTGCAGCGTTTGAAGGCTCGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGTGAACGAAGAAGAGTAGCTGATg         <  2:1953423/92‑1 (MQ=255)
                                                                                ccGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATg         <  1:1199744/93‑1 (MQ=255)
                                                                                  gggTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGga       <  1:2486235/93‑1 (MQ=255)
                                                                                     tGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGagag    <  1:1448140/93‑1 (MQ=255)
                                                                                      gCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAgg   <  1:3802963/93‑1 (MQ=255)
                                                                                      gCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAgg   <  1:6079359/93‑1 (MQ=255)
                                                                                      gCAGCGTGTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAgg   <  1:4458606/93‑1 (MQ=255)
                                                                                          |                                                                                         
TAAGCGGCGATCTCGACCGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGG  >  NC_000913/4075852‑4076031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAGCGGCGATCTCGACCGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCG  >  NC_000913/4075853‑4076034
                                                                                         |                                                                                            
AAGCGGCGATCTCGACCGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTT                                                                                          <  SRR4302533.3302347/94‑1 (MQ=60)
AACCGGCGATCTCGACCCTCTGGCGCGCGGCCGACAAGCACGCGCGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTT                                                                                          <  SRR4302533.391009/94‑1 (MQ=60)
 AGCGGCGATCTCGACCGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTG                                                                                         <  SRR4302533.2942138/94‑1 (MQ=60)
 AGCGGCGATCTCGACCGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTG                                                                                         <  SRR4302533.1985450/94‑1 (MQ=60)
 AGCGGCGATCTCGACCGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGGGCAGCGTTTG                                                                                         >  SRR4302533.5088812/1‑94 (MQ=60)
  GCGGCGATCTCGACCGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGA                                                                                        <  SRR4302533.6343422/94‑1 (MQ=60)
   CGGCGATCTCGACCGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAA                                                                                       <  SRR4302533.3088360/94‑1 (MQ=60)
    GGCGATCTCGACCGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAG                                                                                      <  SRR4302533.1047334/94‑1 (MQ=60)
    GGCGATCTCGACCGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAG                                                                                      <  SRR4302533.4416290/94‑1 (MQ=60)
      CGATCTCGACCGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGC                                                                                    >  SRR4302533.2844957/1‑94 (MQ=60)
       GATCTCGACCGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCA                                                                                   <  SRR4302533.3878967/94‑1 (MQ=60)
       GATCTCGACCGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCA                                                                                   >  SRR4302533.3483262/1‑94 (MQ=60)
       GATCTCGACCGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCA                                                                                   <  SRR4302533.2424901/94‑1 (MQ=60)
        ATCTCGACCGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCTAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCAC                                                                                  <  SRR4302533.3409166/94‑1 (MQ=60)
        ATCTCGACCGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCAC                                                                                  >  SRR4302533.551602/1‑94 (MQ=60)
           TCGACCGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTA                                                                               <  SRR4302533.4533728/94‑1 (MQ=60)
            CGACCGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTAT                                                                              >  SRR4302533.4949268/1‑94 (MQ=60)
            CGACCGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTAT                                                                              <  SRR4302533.6174574/94‑1 (MQ=60)
            CGACCGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTAT                                                                              <  SRR4302533.1126335/94‑1 (MQ=60)
              ACCGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATAT                                                                            <  SRR4302533.3545230/94‑1 (MQ=60)
              ACCGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATAT                                                                            <  SRR4302533.989443/94‑1 (MQ=60)
               CCGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATC                                                                           <  SRR4302533.3318849/94‑1 (MQ=60)
                CGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCG                                                                          <  SRR4302533.5842611/94‑1 (MQ=60)
                   CTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTC                                                                       <  SRR4302533.1962391/94‑1 (MQ=60)
                   CTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGGGCAGCGGTTGAAGGCACGTATATCcttt                                                                       >  SRR4302533.3863632/1‑90 (MQ=60)
                    TGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTTTATCGTTCT                                                                      <  SRR4302533.6046147/94‑1 (MQ=60)
                    TGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCT                                                                      <  SRR4302533.4551705/94‑1 (MQ=60)
                    TGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCT                                                                      <  SRR4302533.4092309/94‑1 (MQ=60)
                    TGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCT                                                                      <  SRR4302533.653363/94‑1 (MQ=60)
                    TGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCT                                                                      <  SRR4302533.5254123/94‑1 (MQ=60)
                    CGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCGTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCT                                                                      <  SRR4302533.1690130/94‑1 (MQ=60)
                      GCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGC                                                                    <  SRR4302533.2590342/94‑1 (MQ=60)
                      GCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGC                                                                    >  SRR4302533.6119540/1‑94 (MQ=60)
                       CGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCC                                                                   <  SRR4302533.1740702/94‑1 (MQ=60)
                        GCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCGGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCC                                                                  <  SRR4302533.2379060/94‑1 (MQ=60)
                        GCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCC                                                                  <  SRR4302533.433028/94‑1 (MQ=60)
                        GCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCC                                                                  <  SRR4302533.5851226/94‑1 (MQ=60)
                         CGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCG                                                                 <  SRR4302533.4339099/94‑1 (MQ=60)
                         CGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCG                                                                 >  SRR4302533.3907000/1‑94 (MQ=60)
                         CGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCG                                                                 <  SRR4302533.3225110/94‑1 (MQ=60)
                             GACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAA                                                             >  SRR4302533.4699028/1‑94 (MQ=60)
                             GACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAA                                                             <  SRR4302533.6034945/94‑1 (MQ=60)
                              ACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAG                                                            <  SRR4302533.5375982/94‑1 (MQ=60)
                                GAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCC                                                          <  SRR4302533.4707874/94‑1 (MQ=60)
                                 AAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCA                                                         <  SRR4302533.4861053/94‑1 (MQ=60)
                                   AAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATT                                                       >  SRR4302533.4854068/1‑94 (MQ=60)
                                   AAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATT                                                       <  SRR4302533.2620489/94‑1 (MQ=60)
                                    AGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTT                                                      <  SRR4302533.5949241/94‑1 (MQ=60)
                                     GCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTG                                                     <  SRR4302533.3365638/94‑1 (MQ=60)
                                       ACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGC                                                   >  SRR4302533.3105097/1‑94 (MQ=60)
                                        CGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCC                                                  <  SRR4302533.198981/94‑1 (MQ=60)
                                          GGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCG                                                <  SRR4302533.5803814/94‑1 (MQ=60)
                                             GCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTAT                                             <  SRR4302533.5061525/94‑1 (MQ=60)
                                               AGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGA                                           <  SRR4302533.4043613/94‑1 (MQ=60)
                                               AGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGA                                           <  SRR4302533.2923796/94‑1 (MQ=60)
                                                GATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAA                                          <  SRR4302533.1278605/94‑1 (MQ=60)
                                                GATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAA                                          <  SRR4302533.4375917/94‑1 (MQ=60)
                                                   GCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAG                                       <  SRR4302533.4811025/94‑1 (MQ=60)
                                                   GCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAG                                       <  SRR4302533.1189210/94‑1 (MQ=60)
                                                   GCAGGTCGAAATCGTTGGCGACGAGACGTCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAG                                       <  SRR4302533.3003484/94‑1 (MQ=60)
                                                     AGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGG                                     <  SRR4302533.512027/94‑1 (MQ=60)
                                                      GGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGC                                    <  SRR4302533.879584/94‑1 (MQ=60)
                                                      GGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGC                                    >  SRR4302533.4622851/1‑94 (MQ=60)
                                                       GTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCG                                   <  SRR4302533.4614928/94‑1 (MQ=60)
                                                       GTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCG                                   <  SRR4302533.4827019/94‑1 (MQ=60)
                                                       GTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCG                                   <  SRR4302533.1316231/94‑1 (MQ=60)
                                                        TCGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGG                                  <  SRR4302533.4284252/94‑1 (MQ=60)
                                                         CGAAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGG                                 <  SRR4302533.2644866/94‑1 (MQ=60)
                                                           AAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAA                               <  SRR4302533.6041614/94‑1 (MQ=60)
                                                           AAATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAA                               <  SRR4302533.6228139/94‑1 (MQ=60)
                                                            AATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAAC                              <  SRR4302533.1336401/94‑1 (MQ=60)
                                                            AATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAAC                              >  SRR4302533.1165885/1‑94 (MQ=60)
                                                             ATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACG                             >  SRR4302533.4274305/1‑94 (MQ=60)
                                                             ATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACG                             >  SRR4302533.2518369/1‑94 (MQ=60)
                                                             ATCTTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACG                             <  SRR4302533.2942299/94‑1 (MQ=60)
                                                               CTGTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAA                           >  SRR4302533.6127603/1‑94 (MQ=60)
                                                                TTTGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAG                          <  SRR4302533.3966596/94‑1 (MQ=60)
                                                                  TGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCTGGAACGAAGAA                        <  SRR4302533.775367/94‑1 (MQ=60)
                                                                  TGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAA                        <  SRR4302533.226517/94‑1 (MQ=60)
                                                                  TGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAA                        <  SRR4302533.2865095/94‑1 (MQ=60)
                                                                  TGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAA                        <  SRR4302533.2041924/94‑1 (MQ=60)
                                                                  TGGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAA                        <  SRR4302533.3805553/94‑1 (MQ=60)
                                                                   GGCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAG                       <  SRR4302533.4028441/94‑1 (MQ=60)
                                                                    GCGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCGCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGA                      <  SRR4302533.3759382/94‑1 (MQ=60)
                                                                     CGACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAG                     <  SRR4302533.3606678/94‑1 (MQ=60)
                                                                       ACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTA                   <  SRR4302533.925799/94‑1 (MQ=60)
                                                                       ACGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTA                   <  SRR4302533.5699705/94‑1 (MQ=60)
                                                                        CGAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAG                  <  SRR4302533.3484060/94‑1 (MQ=60)
                                                                         GAGACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGC                 <  SRR4302533.5377521/94‑1 (MQ=60)
                                                                           GACGCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTG               <  SRR4302533.1971466/94‑1 (MQ=60)
                                                                              GCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATG            >  SRR4302533.2179672/1‑94 (MQ=60)
                                                                              GCCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATG            <  SRR4302533.1040897/94‑1 (MQ=60)
                                                                               CCGGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGG           <  SRR4302533.1206950/94‑1 (MQ=60)
                                                                                 GGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAG         <  SRR4302533.2501554/94‑1 (MQ=60)
                                                                                 GGGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAG         <  SRR4302533.6091413/94‑1 (MQ=60)
                                                                                  GGTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGA        <  SRR4302533.3056166/94‑1 (MQ=60)
                                                                                   GTGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAG       <  SRR4302533.618273/94‑1 (MQ=60)
                                                                                    TGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGG      >  SRR4302533.2855776/1‑94 (MQ=60)
                                                                                    TGCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGG      <  SRR4302533.1456877/94‑1 (MQ=60)
                                                                                     GCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGG     <  SRR4302533.6116126/94‑1 (MQ=60)
                                                                                     GCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGG     <  SRR4302533.3826232/94‑1 (MQ=60)
                                                                                     GCAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGG     >  SRR4302533.2349737/1‑94 (MQ=60)
                                                                                     GCAGCGTGTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGG     <  SRR4302533.4485573/94‑1 (MQ=60)
                                                                                      CAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGG    >  SRR4302533.1658568/1‑94 (MQ=60)
                                                                                      CAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGG    >  SRR4302533.5097089/1‑94 (MQ=60)
                                                                                      CAGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGG    >  SRR4302533.4715624/1‑94 (MQ=60)
                                                                                       AGCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGC   <  SRR4302533.4406706/94‑1 (MQ=60)
                                                                                       AGCGTGTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGC   <  SRR4302533.64656/94‑1 (MQ=60)
                                                                                        GCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCG  >  SRR4302533.6050853/1‑94 (MQ=60)
                                                                                        GCGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCG  <  SRR4302533.1210119/94‑1 (MQ=60)
                                                                                         |                                                                                            
AAGCGGCGATCTCGACCGTCTGGCGCGCGGACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCG  >  NC_000913/4075853‑4076034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑