Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,080,195	A→G	C49R (TGT→CGT)	fdoH ←	formate dehydrogenase‑O, iron‑sulfur subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,080,195	0	A	G	100.0%	862.0 / NA	268	C49R (TGT→CGT)	fdoH	formate dehydrogenase‑O, iron‑sulfur subunit
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (154/114); total (154/114)

Only 100 of 279 total aligned reads displayed.

CCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGG  >  NC_000913/4080105‑4080284

ccGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTCTCGCGAATGTCGTTCCACTCTGAAcgcg                                                                                         >  1:3827364/1‑93 (MQ=255)

  gTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGcc                                                                                       >  1:2439181/1‑93 (MQ=255)

   tCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCa                                                                                      >  1:3646535/1‑93 (MQ=255)

    ccACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCAc                                                                                     >  1:3260007/1‑93 (MQ=255)

      aCGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCt                                                                                   <  1:5771607/93‑1 (MQ=255)

         aTTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGAc                                                                                >  1:3392681/1‑93 (MQ=255)

         aTTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGAc                                                                                >  2:1216757/1‑93 (MQ=255)

         aTTTGGCGCTTAAACCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGAc                                                                                >  1:4824263/1‑93 (MQ=255)

          tttGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACa                                                                               <  1:2629984/93‑1 (MQ=255)

          tttGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACa                                                                               <  2:3578079/93‑1 (MQ=255)

           ttGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAg                                                                              <  2:3085146/93‑1 (MQ=255)

             ggCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGc                                                                            >  1:40444/1‑93 (MQ=255)

               cgcTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCtt                                                                          >  1:5255031/1‑93 (MQ=255)

               cgcTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCtt                                                                          >  2:2562265/1‑93 (MQ=255)

                gcTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCttt                                                                         >  1:1314643/1‑93 (MQ=255)

                gcTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCttt                                                                         >  1:1820144/1‑93 (MQ=255)

                gcTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCttt                                                                         >  2:3883111/1‑93 (MQ=255)

                 cTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTa                                                                        >  1:5603987/1‑93 (MQ=255)

                 cTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTa                                                                        <  1:982584/93‑1 (MQ=255)

                  ttAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTAc                                                                       <  1:2400037/93‑1 (MQ=255)

                  ttAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTAc                                                                       <  1:287808/93‑1 (MQ=255)

                  ttAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTAc                                                                       >  2:643570/1‑93 (MQ=255)

                   tAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACa                                                                      <  2:1050995/93‑1 (MQ=255)

                   tAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACa                                                                      <  2:5876502/93‑1 (MQ=255)

                    aaaTCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAg                                                                     >  2:1786828/1‑93 (MQ=255)

                    aaaTCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAg                                                                     <  1:2545883/93‑1 (MQ=255)

                     aaTCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGc                                                                    <  2:2736834/93‑1 (MQ=255)

                      aTCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGcc                                                                   <  2:397808/93‑1 (MQ=255)

                        cATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGa                                                                 >  1:3589887/1‑93 (MQ=255)

                        cATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGa                                                                 <  1:5712490/93‑1 (MQ=255)

                         aTTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGAt                                                                <  1:1912034/93‑1 (MQ=255)

                          ttGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATa                                                               <  1:2700014/93‑1 (MQ=255)

                          ttGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATa                                                               <  1:1738355/93‑1 (MQ=255)

                          ttGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATa                                                               >  2:2514136/1‑93 (MQ=255)

                            ggggTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCGACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACa                                                             >  1:4117830/1‑93 (MQ=255)

                             gggTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAg                                                            >  1:1625939/1‑93 (MQ=255)

                              ggTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAgg                                                           >  2:5564034/1‑93 (MQ=255)

                              ggTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGCTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAgg                                                           >  1:1563867/1‑93 (MQ=255)

                               gTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAggt                                                          >  1:3693809/1‑93 (MQ=255)

                               gTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAggt                                                          <  2:3411823/93‑1 (MQ=255)

                               gTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAggt                                                          >  1:4451245/1‑93 (MQ=255)

                               gTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAggg                                                          >  2:4141022/1‑92 (MQ=255)

                                   tCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTa                                                      >  2:1677896/1‑93 (MQ=255)

                                    cGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTGCAGCCGATACCGGTGGTaa                                                     <  2:6152224/93‑1 (MQ=25)

                                    cGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTaa                                                     <  2:2062475/93‑1 (MQ=255)

                                    cGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTaa                                                     <  2:1616166/93‑1 (MQ=255)

                                     gTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAAc                                                    <  2:5274652/93‑1 (MQ=255)

                                     ctACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAAc                                                    <  1:895701/92‑1 (MQ=255)

                                       tcacCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCTCCTGACAGGCTTTACAGCCGATACAGGTGGTAACGt                                                  <  1:723182/92‑1 (MQ=255)

                                        cacCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTc                                                 <  2:2591118/93‑1 (MQ=255)

                                        cacCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTc                                                 <  2:4359471/93‑1 (MQ=255)

                                          ccccAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGa                                               <  2:1302373/93‑1 (MQ=255)

                                           cccAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGAt                                              >  2:5650005/1‑93 (MQ=255)

                                            ccAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATg                                             >  2:4798644/1‑93 (MQ=255)

                                             cAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGa                                            >  1:4549893/1‑93 (MQ=255)

                                             cAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGa                                            >  1:5834224/1‑93 (MQ=255)

                                               aTGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGt                                          >  1:1390248/1‑93 (MQ=255)

                                               aTGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGt                                          <  1:531133/93‑1 (MQ=255)

                                               aTGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGt                                          >  2:2249936/1‑93 (MQ=255)

                                                 gTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGttt                                        >  2:1532918/1‑93 (MQ=255)

                                                 gTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGttt                                        >  1:619144/1‑93 (MQ=255)

                                                  ttATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTc                                       <  2:1398896/93‑1 (MQ=255)

                                                  ttATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTc                                       >  1:6248169/1‑93 (MQ=255)

                                                       gCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCAc                                  >  2:27690/1‑93 (MQ=255)

                                                          gACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCActtc                               <  1:2670930/93‑1 (MQ=255)

                                                           aCGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGCGTTTCGCCActtct                              >  1:4789061/1‑93 (MQ=255)

                                                           aCGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCActtct                              >  2:5697256/1‑93 (MQ=255)

                                                            cGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCActtctt                             >  2:895379/1‑93 (MQ=255)

                                                               tATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCt                          <  2:452736/93‑1 (MQ=255)

                                                               tATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCt                          <  1:4659815/93‑1 (MQ=255)

                                                                aTCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTGCTg                         >  1:2235645/1‑93 (MQ=255)

                                                                aTCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTg                         <  2:4986657/93‑1 (MQ=255)

                                                                aTCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTg                         >  1:4105355/1‑93 (MQ=255)

                                                                 tCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTgg                        >  2:197687/1‑93 (MQ=255)

                                                                 tCGCGAATGTCGTCCCACTTGGAACGCGCCCCCGGACAGCCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTgg                        <  2:5223673/93‑1 (MQ=18)

                                                                   gcgAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGaa                      <  2:2632958/93‑1 (MQ=255)

                                                                   gcgAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGaa                      >  2:3246725/1‑93 (MQ=255)

                                                                    cgAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAg                     >  2:5165080/1‑93 (MQ=255)

                                                                    cgAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAg                     >  2:2976673/1‑93 (MQ=255)

                                                                    cgAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAg                     >  2:117412/1‑93 (MQ=255)

                                                                    cgAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAg                     >  2:2869183/1‑93 (MQ=255)

                                                                       aTGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTcc                  >  2:2994674/1‑93 (MQ=255)

                                                                       aTGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTcc                  <  2:1917297/93‑1 (MQ=255)

                                                                       aTGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTcc                  >  1:4908190/1‑93 (MQ=255)

                                                                       aTGTCGTTCCACTCTGAACGCCCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTcc                  <  2:4286947/93‑1 (MQ=255)

                                                                        tGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTccc                 <  1:5078507/93‑1 (MQ=255)

                                                                         gTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCcg                <  1:657363/93‑1 (MQ=255)

                                                                                cACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGa         <  1:317298/93‑1 (MQ=255)

                                                                                cACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGa         >  2:3676333/1‑93 (MQ=255)

                                                                                cACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGa         <  1:1514374/93‑1 (MQ=255)

                                                                                cACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTATTCCTGGAAGTCCCGCGCCTGa         >  1:2165308/1‑93 (MQ=255)

                                                                                 cctctGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAg        <  1:1080421/92‑1 (MQ=255)

                                                                                 aCTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGCAGTCCCGCGCCTGAg        >  2:3078186/1‑93 (MQ=255)

                                                                                 aCTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAg        >  2:2416847/1‑93 (MQ=255)

                                                                                 aCTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAg        >  1:5997933/1‑93 (MQ=255)

                                                                                  ctctGGACGCGCAACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAgg       <  1:2779915/93‑1 (MQ=255)

                                                                                   tctGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGgc      >  1:3791893/1‑93 (MQ=255)

                                                                                     tGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGgcgc    >  1:6212104/1‑93 (MQ=255)

                                                                                      gAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGcgcg   >  1:5378342/1‑93 (MQ=255)

                                                                                       aaCGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCgg  <  1:5757231/93‑1 (MQ=255)

CCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGG  >  NC_000913/4080105‑4080284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGG  >  NC_000913/4080103‑4080285

TACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGC                                                                                           >  SRR4302533.4274914/1‑94 (MQ=60)

 ACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTCTCGCGAATGTCGTTCCACTCTGAACGCG                                                                                          >  SRR4302533.3850762/1‑94 (MQ=60)

 ACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCG                                                                                          >  SRR4302533.6127088/1‑94 (MQ=60)

 ACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCG                                                                                          >  SRR4302533.5804058/1‑94 (MQ=60)

   CGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCC                                                                                        >  SRR4302533.2454245/1‑94 (MQ=60)

    GTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCA                                                                                       >  SRR4302533.3668956/1‑94 (MQ=60)

     TCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCAC                                                                                      >  SRR4302533.3280117/1‑94 (MQ=60)

      CCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACC                                                                                     >  SRR4302533.4913389/1‑94 (MQ=60)

       CACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCT                                                                                    <  SRR4302533.5744811/94‑1 (MQ=60)

        ACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTG                                                                                   <  SRR4302533.5806458/94‑1 (MQ=60)

          GATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGAC                                                                                 >  SRR4302533.3413632/1‑94 (MQ=60)

          GATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGAC                                                                                 >  SRR4302533.5048122/1‑94 (MQ=60)

          GATTTGGCGCTTAAACCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGAC                                                                                 >  SRR4302533.4853485/1‑94 (MQ=60)

           ATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACA                                                                                <  SRR4302533.989507/94‑1 (MQ=60)

           ATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACA                                                                                <  SRR4302533.4665251/94‑1 (MQ=60)

           ATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACA                                                                                <  SRR4302533.1613232/94‑1 (MQ=60)

            TTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAG                                                                               <  SRR4302533.2646147/94‑1 (MQ=60)

            TTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAG                                                                               <  SRR4302533.1514643/94‑1 (MQ=60)

             TTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGG                                                                              <  SRR4302533.5978147/94‑1 (MQ=60)

              TGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGC                                                                             >  SRR4302533.40718/1‑94 (MQ=60)

              TGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGC                                                                             <  SRR4302533.697631/94‑1 (MQ=60)

                GCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTT                                                                           <  SRR4302533.3823213/94‑1 (MQ=60)

                GCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTT                                                                           <  SRR4302533.3712532/94‑1 (MQ=60)

                GCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTT                                                                           >  SRR4302533.5286599/1‑94 (MQ=60)

                 CGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTT                                                                          >  SRR4302533.5838922/1‑94 (MQ=60)

                 CGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTT                                                                          >  SRR4302533.1831261/1‑94 (MQ=60)

                 CGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTT                                                                          >  SRR4302533.1322530/1‑94 (MQ=60)

                  GCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTA                                                                         <  SRR4302533.1875675/94‑1 (MQ=60)

                  GCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTA                                                                         >  SRR4302533.5637844/1‑94 (MQ=60)

                   CTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTAC                                                                        <  SRR4302533.1662682/94‑1 (MQ=60)

                   CTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTAC                                                                        <  SRR4302533.988341/94‑1 (MQ=60)

                   CTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTAC                                                                        >  SRR4302533.5321878/1‑94 (MQ=60)

                    TTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACA                                                                       <  SRR4302533.2414857/94‑1 (MQ=60)

                    TTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACA                                                                       <  SRR4302533.289567/94‑1 (MQ=60)

                    TTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACA                                                                       >  SRR4302533.639294/1‑94 (MQ=60)

                     TAAATCATTGGGGTTGTCGTACGCCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAG                                                                      <  SRR4302533.5497979/94‑1 (MQ=60)

                      AAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGC                                                                     <  SRR4302533.2561547/94‑1 (MQ=60)

                      AAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGC                                                                     <  SRR4302533.3718662/94‑1 (MQ=60)

                      AAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGC                                                                     <  SRR4302533.5755084/94‑1 (MQ=60)

                       AATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCC                                                                    >  SRR4302533.5970567/1‑94 (MQ=60)

                       AATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCC                                                                    <  SRR4302533.265053/94‑1 (MQ=60)

                        ATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCG                                                                   >  SRR4302533.320466/1‑94 (MQ=60)

                         TCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGA                                                                  >  SRR4302533.4558280/1‑94 (MQ=60)

                         TCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGA                                                                  >  SRR4302533.3611987/1‑94 (MQ=60)

                         TCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGA                                                                  <  SRR4302533.5388130/94‑1 (MQ=60)

                          CATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGAT                                                                 <  SRR4302533.5746997/94‑1 (MQ=60)

                          CATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGAT                                                                 >  SRR4302533.2635201/1‑94 (MQ=60)

                          CATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGAT                                                                 <  SRR4302533.4591675/94‑1 (MQ=60)

                          CATTGGGGTTCTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGAT                                                                 <  SRR4302533.285429/94‑1 (MQ=60)

                           ATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATA                                                                <  SRR4302533.1923699/94‑1 (MQ=60)

                           ATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATA                                                                >  SRR4302533.732973/1‑94 (MQ=60)

                            TTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATAC                                                               <  SRR4302533.1748900/94‑1 (MQ=60)

                            TTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATAC                                                               <  SRR4302533.2716580/94‑1 (MQ=60)

                             TGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCGACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACA                                                              >  SRR4302533.4142842/1‑94 (MQ=60)

                              GGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAG                                                             >  SRR4302533.1635788/1‑94 (MQ=60)

                               GGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGCTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGG                                                            >  SRR4302533.1573281/1‑94 (MQ=60)

                                GGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGT                                                           >  SRR4302533.3716500/1‑94 (MQ=60)

                                GGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGT                                                           >  SRR4302533.4478158/1‑94 (MQ=60)

                                GGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGT                                                           >  SRR4302533.3855620/1‑94 (MQ=60)

                                  TTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGG                                                         <  SRR4302533.906369/94‑1 (MQ=60)

                                   TGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGagga                                                        >  SRR4302533.2861189/1‑90 (MQ=60)

                                   TGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGT                                                        <  SRR4302533.2198929/94‑1 (MQ=60)

                                       CTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACG                                                    <  SRR4302533.900962/94‑1 (MQ=60)

                                         TCACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCTCCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTC                                                  <  SRR4302533.727458/94‑1 (MQ=60)

                                         ACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTC                                                  <  SRR4302533.1528736/94‑1 (MQ=60)

                                          CACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTTG                                                 >  SRR4302533.2771942/1‑94 (MQ=60)

                                          CACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCG                                                 >  SRR4302533.3506123/1‑94 (MQ=60)

                                          CACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCG                                                 >  SRR4302533.2416775/1‑94 (MQ=60)

                                           ACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGA                                                >  SRR4302533.821583/1‑94 (MQ=60)

                                            CCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGAT                                               >  SRR4302533.4966344/1‑94 (MQ=60)

                                            CCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGAT                                               >  SRR4302533.1952351/1‑94 (MQ=60)

                                            CCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGAT                                               <  SRR4302533.5324184/94‑1 (MQ=60)

                                             CCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATG                                              >  SRR4302533.1323372/1‑94 (MQ=60)

                                             CCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATG                                              >  SRR4302533.474366/1‑94 (MQ=60)

                                             CCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATG                                              >  SRR4302533.5660201/1‑94 (MQ=60)

                                             CCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATG                                              >  SRR4302533.1648254/1‑94 (MQ=60)

                                              CCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGA                                             >  SRR4302533.5256665/1‑94 (MQ=60)

                                              CCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGA                                             >  SRR4302533.5869483/1‑94 (MQ=60)

                                              CCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGA                                             <  SRR4302533.6303362/94‑1 (MQ=60)

                                              CCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGA                                             >  SRR4302533.3486425/1‑94 (MQ=60)

                                              CCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGA                                             >  SRR4302533.4577482/1‑94 (MQ=60)

                                               CAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAG                                            <  SRR4302533.2405898/94‑1 (MQ=60)

                                               CAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAG                                            <  SRR4302533.4878068/94‑1 (MQ=60)

                                               CAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAG                                            >  SRR4302533.679437/1‑94 (MQ=60)

                                               CAATGTTATTGCCGACGGCATCGCGAATGTAGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTCACGTCGATGAG                                            >  SRR4302533.5598476/1‑94 (MQ=60)

                                                AATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGT                                           >  SRR4302533.1398622/1‑94 (MQ=60)

                                                AATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGT                                           <  SRR4302533.1447154/94‑1 (MQ=60)

                                                 ATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTT                                          >  SRR4302533.5128402/1‑94 (MQ=60)

                                                 ATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTT                                          <  SRR4302533.534291/94‑1 (MQ=60)

                                                 ATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGGT                                          >  SRR4302533.451899/1‑94 (MQ=60)

                                                  TGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTT                                         >  SRR4302533.622783/1‑94 (MQ=60)

                                                  TGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTT                                         >  SRR4302533.622782/1‑94 (MQ=60)

                                                   GTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTC                                        >  SRR4302533.6285557/1‑94 (MQ=60)

                                                   GTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTC                                        >  SRR4302533.6285556/1‑94 (MQ=60)

                                                   GTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTC                                        >  SRR4302533.4743395/1‑94 (MQ=60)

                                                   GTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTC                                        >  SRR4302533.2752294/1‑94 (MQ=60)

                                                    TTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCG                                       >  SRR4302533.4121120/1‑94 (MQ=60)

                                                    TTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCG                                       <  SRR4302533.6247853/94‑1 (MQ=60)

                                                    TTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCG                                       <  SRR4302533.1881284/94‑1 (MQ=60)

                                                    TTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCG                                       >  SRR4302533.904986/1‑94 (MQ=60)

                                                    TTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCG                                       >  SRR4302533.4189858/1‑94 (MQ=60)

                                                     TATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGC                                      >  SRR4302533.3822123/1‑94 (MQ=60)

                                                     TATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGC                                      >  SRR4302533.3356909/1‑94 (MQ=60)

                                                       TTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCA                                    <  SRR4302533.5831929/94‑1 (MQ=60)

                                                         GCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACT                                  <  SRR4302533.2689456/94‑1 (MQ=60)

                                                          CCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTT                                 >  SRR4302533.2069372/1‑94 (MQ=60)

                                                           CGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTC                                <  SRR4302533.2440994/94‑1 (MQ=60)

                                                           CGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTC                                >  SRR4302533.2705143/1‑94 (MQ=60)

                                                            GACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGCGTTTCGCCACTTCT                               >  SRR4302533.4818067/1‑94 (MQ=60)

                                                            GACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCT                               >  SRR4302533.2600859/1‑94 (MQ=60)

                                                            GACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCT                               <  SRR4302533.2687312/94‑1 (MQ=60)

                                                              CGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTC                             >  SRR4302533.1822360/1‑94 (MQ=60)

                                                                GTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCT                           <  SRR4302533.1345297/94‑1 (MQ=60)

                                                                 TATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTGCTG                          >  SRR4302533.2249467/1‑94 (MQ=60)

                                                                 TATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTG                          >  SRR4302533.4130324/1‑94 (MQ=60)

                                                                 TATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTG                          <  SRR4302533.4688055/94‑1 (MQ=60)

                                                                 TATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTG                          >  SRR4302533.4130323/1‑94 (MQ=60)

                                                                  ATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGG                         <  SRR4302533.3948100/94‑1 (MQ=60)

                                                                   TCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGA                        <  SRR4302533.2546668/94‑1 (MQ=60)

                                                                   TCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGA                        <  SRR4302533.254617/94‑1 (MQ=60)

                                                                     GCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAG                      <  SRR4302533.1181315/94‑1 (MQ=60)

                                                                       GAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTC                    <  SRR4302533.1542303/94‑1 (MQ=60)

                                                                       GAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTC                    >  SRR4302533.4109707/1‑94 (MQ=60)

                                                                        AATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCC                   >  SRR4302533.3056044/1‑94 (MQ=60)

                                                                        AATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCC                   >  SRR4302533.4937927/1‑94 (MQ=60)

                                                                         ATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCC                  >  SRR4302533.4249807/1‑94 (MQ=60)

                                                                         AGGTCTTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCCCTTCTTCCTGGAAGTCCC                  <  SRR4302533.2311353/94‑1 (MQ=60)

                                                                          TGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCG                 <  SRR4302533.1801367/94‑1 (MQ=60)

                                                                          TGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCG                 <  SRR4302533.2782627/94‑1 (MQ=60)

                                                                          TGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCG                 <  SRR4302533.5109202/94‑1 (MQ=60)

                                                                           GTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGC                <  SRR4302533.661240/94‑1 (MQ=60)

                                                                              GTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCC             >  SRR4302533.3801880/1‑94 (MQ=60)

                                                                               TTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCT            >  SRR4302533.3644497/1‑94 (MQ=60)

                                                                               TTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCT            >  SRR4302533.6064963/1‑94 (MQ=60)

                                                                                TCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCccgccca           >  SRR4302533.3294420/1‑87 (MQ=60)

                                                                                 CCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTATTCCTGGAAGTCCCGCGCCTGA          >  SRR4302533.2178627/1‑94 (MQ=60)

                                                                                  CACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAG         <  SRR4302533.319241/94‑1 (MQ=60)

                                                                                  CACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAG         >  SRR4302533.6034260/1‑94 (MQ=60)

                                                                                  CACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAG         <  SRR4302533.3649878/94‑1 (MQ=60)

                                                                                  CACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAG         <  SRR4302533.1523497/94‑1 (MQ=60)

                                                                                   CCTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGG        <  SRR4302533.1086793/94‑1 (MQ=60)

                                                                                   ACTCTGACCGCGCCACCTGACAGGCTTTGCAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGG        <  SRR4302533.5763521/94‑1 (MQ=60)

                                                                                    CTCTGGACGCGCAACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGC       <  SRR4302533.2796992/94‑1 (MQ=60)

                                                                                    CTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGC       >  SRR4302533.4839072/1‑94 (MQ=60)

                                                                                    CTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGC       >  SRR4302533.3815106/1‑94 (MQ=60)

                                                                                     TCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCG      <  SRR4302533.3061717/94‑1 (MQ=60)

                                                                                      CTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGC     >  SRR4302533.6249355/1‑94 (MQ=60)

                                                                                       TGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCG    >  SRR4302533.5410661/1‑94 (MQ=60)

                                                                                         AACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGG  <  SRR4302533.5791974/94‑1 (MQ=60)

TACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGG  >  NC_000913/4080103‑4080285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`