Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,103,641	T→C	intergenic (‑390/‑408)	cpxR ← / → yiiP	transcriptional regulator in 2‑component system/putative transport system permease protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,103,641	0	T	C	99.6%	820.6 / ‑4.7	256	intergenic (‑390/‑408)	cpxR/yiiP	transcriptional regulator in 2‑component system/putative transport system permease protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (133/122); minor base A (0/1); total (133/123)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.48e-01

Only 100 of 261 total aligned reads displayed.

TAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAATGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGG  >  NC_000913/4103551‑4103730

 aaCAGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCa                                                                                        <  1:3338149/93‑1 (MQ=255)

   ccGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATc                                                                                      >  1:3583217/1‑93 (MQ=255)

     gAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGc                                                                                    <  2:1220858/93‑1 (MQ=255)

     gAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGc                                                                                    >  1:987445/1‑93 (MQ=255)

      aaCATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGcc                                                                                   >  2:5556035/1‑93 (MQ=255)

       aCATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCt                                                                                  >  1:6073688/1‑93 (MQ=255)

        cATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCtt                                                                                 <  2:446179/93‑1 (MQ=255)

         aTCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTg                                                                                <  1:2771282/93‑1 (MQ=255)

           cAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTc                                                                              >  1:6002537/1‑93 (MQ=255)

             gCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCAc                                                                            <  2:467812/93‑1 (MQ=255)

               gTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCg                                                                          >  1:742292/1‑93 (MQ=255)

                tCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGc                                                                         >  2:821038/1‑93 (MQ=255)

                tCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGc                                                                         <  1:1922986/93‑1 (MQ=255)

                 cagcagATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCa                                                                        >  1:116651/1‑93 (MQ=255)

                  agcagATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAg                                                                       >  2:674880/1‑93 (MQ=255)

                  agcagATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAg                                                                       <  1:1828453/93‑1 (MQ=255)

                  agcagATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATAGCCTTGTCACCGCAg                                                                       >  2:3070479/1‑93 (MQ=255)

                     agATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGaaa                                                                    >  2:1070646/1‑93 (MQ=255)

                      gATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGGTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGaaaa                                                                   >  2:3682808/1‑93 (MQ=255)

                         gCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAAttt                                                                >  2:1863402/1‑93 (MQ=255)

                          cGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAAtttt                                                               <  1:4819903/93‑1 (MQ=255)

                           gagaTCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTtg                                                              >  2:2038130/1‑93 (MQ=255)

                             gaTCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTtgat                                                            >  1:4491264/1‑93 (MQ=255)

                              aTCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTtgatg                                                           >  1:2654876/1‑93 (MQ=255)

                              aTCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTtgatg                                                           <  1:1414463/93‑1 (MQ=255)

                              aTCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTtgatg                                                           >  1:3426308/1‑93 (MQ=255)

                               tCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTtgatga                                                          >  2:2497648/1‑93 (MQ=255)

                               tCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTtgatga                                                          >  2:3276794/1‑93 (MQ=255)

                               tCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTtgatga                                                          >  1:4147833/1‑93 (MQ=255)

                               tCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTtgatga                                                          >  1:1258561/1‑93 (MQ=255)

                               tCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTtgatga                                                          <  2:5826164/93‑1 (MQ=255)

                               acTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTtgatga                                                          <  2:1501120/92‑1 (MQ=255)

                                cTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGaa                                                         <  2:4942017/93‑1 (MQ=255)

                                  tATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGaaaa                                                       <  2:5114728/93‑1 (MQ=255)

                                    tGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACg                                                     >  1:1750784/1‑93 (MQ=255)

                                      cAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCt                                                   <  2:5403354/93‑1 (MQ=255)

                                      cAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCt                                                   <  2:615498/93‑1 (MQ=255)

                                       aaCAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCtg                                                  >  2:2931329/1‑93 (MQ=255)

                                        aCAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCtgt                                                 >  2:6305273/1‑93 (MQ=255)

                                        aCAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCtgt                                                 >  2:1817798/1‑93 (MQ=255)

                                        aCAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCtgt                                                 >  1:2443746/1‑93 (MQ=255)

                                         cAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCtgtg                                                <  1:5577739/93‑1 (MQ=255)

                                          aGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTgc                                               >  2:2872805/1‑93 (MQ=255)

                                          aGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTgc                                               <  1:5275232/93‑1 (MQ=255)

                                          aGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTgc                                               <  2:4832542/93‑1 (MQ=255)

                                          aGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAAAGCTGTgc                                               >  2:3790411/1‑93 (MQ=255)

                                           ggCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTgcg                                              >  1:2038992/1‑93 (MQ=255)

                                            gCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTgcgc                                             >  2:3140425/1‑93 (MQ=255)

                                              ccGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTgcgcgc                                           <  1:1653825/93‑1 (MQ=255)

                                               cGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGca                                          <  2:3866993/93‑1 (MQ=255)

                                                 gCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGcaca                                        <  2:5286946/93‑1 (MQ=255)

                                                 gCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGcaca                                        >  1:1986894/1‑93 (MQ=255)

                                                   aCGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAgg                                      <  1:2594877/93‑1 (MQ=255)

                                                    cGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGc                                     <  1:372889/93‑1 (MQ=255)

                                                    cGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGc                                     >  2:2211710/1‑93 (MQ=255)

                                                     gAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCa                                    >  2:2574882/1‑93 (MQ=255)

                                                      aaCAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAg                                   >  1:5039132/1‑93 (MQ=255)

                                                       aCAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGa                                  >  1:93458/1‑93 (MQ=255)

                                                        cAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGaa                                 <  2:4423358/93‑1 (MQ=255)

                                                          gCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGaaaa                               >  2:1882505/1‑93 (MQ=255)

                                                           cctcctGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCACAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGaaaaa                              >  1:3854545/1‑93 (MQ=255)

                                                            ctcctGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGaaaaaa                             <  1:4362431/93‑1 (MQ=255)

                                                            ctcctGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGaaaaaa                             <  2:6053974/93‑1 (MQ=255)

                                                            ctcctGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGaaaaaa                             <  2:126694/93‑1 (MQ=255)

                                                             tcctGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAAt                            >  1:2289147/1‑93 (MQ=255)

                                                             tcctGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAAt                            >  2:3905520/1‑93 (MQ=255)

                                                               ctGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATgg                          <  2:477841/93‑1 (MQ=255)

                                                               ctGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATgg                          >  2:4345387/1‑93 (MQ=255)

                                                                tGTTAATGTTCGAGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAAtaga                         >  2:5067134/1‑90 (MQ=255)

                                                                tGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACGGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGc                         >  1:3387213/1‑93 (MQ=255)

                                                                tGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGc                         >  1:2360427/1‑93 (MQ=255)

                                                                tGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGc                         <  1:6240173/93‑1 (MQ=255)

                                                                  ttAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGa                       <  1:2911318/93‑1 (MQ=255)

                                                                    aaTGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAAt                     <  1:5363374/93‑1 (MQ=255)

                                                                     aTGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATg                    <  2:1619618/93‑1 (MQ=255)

                                                                     aTGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATg                    <  1:1938133/93‑1 (MQ=255)

                                                                         tAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCa                <  2:4565478/93‑1 (MQ=255)

                                                                           gCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCaaa              <  2:2112662/93‑1 (MQ=255)

                                                                            cGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAAt             >  1:5884629/1‑93 (MQ=255)

                                                                            cGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAAt             <  2:2746659/93‑1 (MQ=255)

                                                                            cGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAAt             <  1:4080891/93‑1 (MQ=255)

                                                                            cGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAAt             <  2:5900480/93‑1 (MQ=255)

                                                                             gAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACCGGCAGAAAAAATGGCGAATGAGCAAAtt            >  1:1010893/1‑93 (MQ=255)

                                                                             gAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAAtt            <  1:1576121/93‑1 (MQ=255)

                                                                              aaCTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTg           >  1:5487238/1‑93 (MQ=255)

                                                                               aCTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGc          <  2:4238843/93‑1 (MQ=255)

                                                                               aCTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGc          >  1:5386519/1‑93 (MQ=255)

                                                                               aCTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGc          <  2:6103501/93‑1 (MQ=255)

                                                                                 tGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTc        >  2:5974464/1‑93 (MQ=255)

                                                                                 tGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTc        <  2:5437536/93‑1 (MQ=255)

                                                                                  ggAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCg       <  2:4146519/93‑1 (MQ=255)

                                                                                  ggAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCg       >  2:5622035/1‑93 (MQ=255)

                                                                                  ggAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCg       >  2:44028/1‑93 (MQ=255)

                                                                                   gagaCAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGt      >  1:4687873/1‑93 (MQ=255)

                                                                                   gagaCAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGt      <  2:1688828/93‑1 (MQ=255)

                                                                                   gagaCAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGt      >  1:1488456/1‑93 (MQ=255)

                                                                                    agaCAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTc     >  1:1083691/1‑93 (MQ=255)

                                                                                    agaCAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTc     <  1:5049421/93‑1 (MQ=255)

                                                                                      aCAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAg   <  1:4535596/93‑1 (MQ=255)

                                                                                       cAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAgg  >  1:3026380/1‑93 (MQ=255)

TAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAATGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGG  >  NC_000913/4103551‑4103730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAATGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGA  >  NC_000913/4103549‑4103734

TTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACG                                                                                              <  SRR4302533.4087091/94‑1 (MQ=60)

 TTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGC                                                                                             <  SRR4302533.4534842/94‑1 (MQ=60)

  TAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCA                                                                                            <  SRR4302533.1938993/94‑1 (MQ=60)

   AACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCAT                                                                                           <  SRR4302533.1602944/94‑1 (MQ=60)

   AACAGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCAT                                                                                           <  SRR4302533.3358783/94‑1 (MQ=60)

    ACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATC                                                                                          >  SRR4302533.3605285/1‑94 (MQ=60)

      CGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGC                                                                                        >  SRR4302533.993224/1‑94 (MQ=60)

       GAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCC                                                                                       >  SRR4302533.6127200/1‑94 (MQ=60)

        AACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCT                                                                                      >  SRR4302533.6110429/1‑94 (MQ=60)

        AACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCT                                                                                      >  SRR4302533.2330860/1‑94 (MQ=60)

        AACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCT                                                                                      >  SRR4302533.4547130/1‑94 (MQ=60)

          CATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTG                                                                                    >  SRR4302533.6034402/1‑94 (MQ=60)

          CATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTG                                                                                    <  SRR4302533.3417089/94‑1 (MQ=60)

           ATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGT                                                                                   >  SRR4302533.2766290/1‑94 (MQ=60)

           ATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGT                                                                                   <  SRR4302533.2788285/94‑1 (MQ=60)

            TCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTC                                                                                  >  SRR4302533.6038904/1‑94 (MQ=60)

              AGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCAC                                                                                <  SRR4302533.2621433/94‑1 (MQ=60)

               GCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACC                                                                               >  SRR4302533.5930257/1‑94 (MQ=60)

                CGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCG                                                                              >  SRR4302533.746662/1‑94 (MQ=60)

                 GTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGC                                                                             <  SRR4302533.1663095/94‑1 (MQ=60)

                 GTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGC                                                                             >  SRR4302533.4216294/1‑94 (MQ=60)

                  TCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCA                                                                            <  SRR4302533.1934720/94‑1 (MQ=60)

                  TCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCA                                                                            >  SRR4302533.117394/1‑94 (MQ=60)

                   CAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAG                                                                           <  SRR4302533.5372880/94‑1 (MQ=60)

                    AGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGA                                                                          <  SRR4302533.5019074/94‑1 (MQ=60)

                    AGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGA                                                                          <  SRR4302533.1839619/94‑1 (MQ=60)

                    AGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGA                                                                          >  SRR4302533.6094477/1‑94 (MQ=60)

                     GCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAA                                                                         <  SRR4302533.5000747/94‑1 (MQ=60)

                      CAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAA                                                                        <  SRR4302533.196836/94‑1 (MQ=60)

                       AGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAA                                                                       >  SRR4302533.4151905/1‑94 (MQ=60)

                        GATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAAT                                                                      <  SRR4302533.766971/94‑1 (MQ=60)

                         ATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATT                                                                     >  SRR4302533.1679067/1‑94 (MQ=60)

                          TGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTT                                                                    >  SRR4302533.2344907/1‑94 (MQ=60)

                          TGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACCGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTT                                                                    >  SRR4302533.2810453/1‑94 (MQ=60)

                           GCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTT                                                                   <  SRR4302533.5869694/94‑1 (MQ=60)

                            CGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTG                                                                  <  SRR4302533.4849110/94‑1 (MQ=60)

                             GAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGA                                                                 <  SRR4302533.4531628/94‑1 (MQ=60)

                             GAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGA                                                                 >  SRR4302533.518017/1‑94 (MQ=60)

                              AGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGATACAACGCATCGCCTTGTCACCGCAGAAAATTTTGAT                                                                >  SRR4302533.392259/1‑94 (MQ=60)

                              AGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGAT                                                                >  SRR4302533.4518465/1‑94 (MQ=60)

                              AGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGAT                                                                >  SRR4302533.1446959/1‑94 (MQ=60)

                              AGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGAT                                                                >  SRR4302533.1160268/1‑94 (MQ=60)

                              AGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGAT                                                                <  SRR4302533.3364453/94‑1 (MQ=60)

                               GATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATG                                                               >  SRR4302533.3447474/1‑94 (MQ=60)

                               GATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATG                                                               >  SRR4302533.3371434/1‑94 (MQ=60)

                               GATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATG                                                               >  SRR4302533.2671184/1‑94 (MQ=60)

                                ATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGA                                                              >  SRR4302533.1266089/1‑94 (MQ=60)

                                ATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGA                                                              >  SRR4302533.4172963/1‑94 (MQ=60)

                                ATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGA                                                              <  SRR4302533.1423019/94‑1 (MQ=60)

                                    TATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAAC                                                          <  SRR4302533.4642947/94‑1 (MQ=60)

                                     ATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTTGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACG                                                         <  SRR4302533.1536792/94‑1 (MQ=60)

                                     ATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACG                                                         <  SRR4302533.3602438/94‑1 (MQ=60)

                                     ATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACG                                                         <  SRR4302533.3657129/94‑1 (MQ=60)

                                     ATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACG                                                         >  SRR4302533.1761445/1‑94 (MQ=60)

                                       GCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCT                                                       <  SRR4302533.5433553/94‑1 (MQ=60)

                                       GCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCT                                                       <  SRR4302533.4367072/94‑1 (MQ=60)

                                       GCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCT                                                       <  SRR4302533.2759514/94‑1 (MQ=60)

                                        CAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTG                                                      <  SRR4302533.1041778/94‑1 (MQ=60)

                                        CAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTG                                                      >  SRR4302533.5406614/1‑94 (MQ=60)

                                         AACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGT                                                     >  SRR4302533.3757478/1‑94 (MQ=60)

                                         AACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGT                                                     >  SRR4302533.2458830/1‑94 (MQ=60)

                                         AACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGT                                                     >  SRR4302533.4649802/1‑94 (MQ=60)

                                         AACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAAAGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGT                                                     <  SRR4302533.989873/94‑1 (MQ=60)

                                           CAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGC                                                   <  SRR4302533.5611448/94‑1 (MQ=60)

                                            AGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCG                                                  <  SRR4302533.5306862/94‑1 (MQ=60)

                                            AGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCG                                                  >  SRR4302533.2051567/1‑94 (MQ=60)

                                               CCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGC                                               >  SRR4302533.1315124/1‑94 (MQ=60)

                                                CCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCA                                              >  SRR4302533.5494747/1‑94 (MQ=60)

                                                CCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCA                                              <  SRR4302533.1663840/94‑1 (MQ=60)

                                                  GGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACA                                            >  SRR4302533.17744/1‑94 (MQ=60)

                                                  GGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACA                                            >  SRR4302533.1999049/1‑94 (MQ=60)

                                                   GCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAG                                           <  SRR4302533.1456379/94‑1 (MQ=60)

                                                   GCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAG                                           <  SRR4302533.384047/94‑1 (MQ=60)

                                                   GCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAG                                           <  SRR4302533.6311095/94‑1 (MQ=60)

                                                    CACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGG                                          >  SRR4302533.293954/1‑94 (MQ=60)

                                                     ACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGC                                         >  SRR4302533.5182430/1‑94 (MQ=60)

                                                     ACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGC                                         <  SRR4302533.2610825/94‑1 (MQ=60)

                                                     ACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGC                                         >  SRR4302533.5076631/1‑94 (MQ=60)

                                                      CGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCA                                        <  SRR4302533.375141/94‑1 (MQ=60)

                                                       GAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAG                                       >  SRR4302533.5069642/1‑94 (MQ=60)

                                                       GAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAG                                       <  SRR4302533.2904947/94‑1 (MQ=60)

                                                        AACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGA                                      >  SRR4302533.94047/1‑94 (MQ=60)

                                                            GCCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCACAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAA                                  >  SRR4302533.3878086/1‑94 (MQ=60)

                                                             CCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAA                                 >  SRR4302533.3099073/1‑94 (MQ=60)

                                                             CCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAA                                 >  SRR4302533.1999908/1‑94 (MQ=60)

                                                             CCTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAA                                 >  SRR4302533.2402159/1‑94 (MQ=60)

                                                              CTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAAT                                >  SRR4302533.2303321/1‑94 (MQ=60)

                                                              CTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAAT                                <  SRR4302533.4388763/94‑1 (MQ=60)

                                                              CTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAAT                                <  SRR4302533.1864821/94‑1 (MQ=60)

                                                              CTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAAT                                <  SRR4302533.3988412/94‑1 (MQ=60)

                                                              CTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAAT                                >  SRR4302533.964391/1‑94 (MQ=60)

                                                              CTCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAAT                                <  SRR4302533.1971364/94‑1 (MQ=60)

                                                               TCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATG                               <  SRR4302533.1205650/94‑1 (MQ=60)

                                                               TCCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATG                               <  SRR4302533.5545538/94‑1 (MQ=60)

                                                                CCTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAACCGCTGTGCGCGCACAGGCAGAAAAAATGG                              <  SRR4302533.3117115/94‑1 (MQ=60)

                                                                 CTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACGGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGC                             >  SRR4302533.3408128/1‑94 (MQ=60)

                                                                 CTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGC                             >  SRR4302533.6159317/1‑94 (MQ=60)

                                                                 CTGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGC                             >  SRR4302533.2375015/1‑94 (MQ=60)

                                                                  TGTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCG                            <  SRR4302533.6277538/94‑1 (MQ=60)

                                                                   GTTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGA                           >  SRR4302533.2477342/1‑94 (MQ=60)

                                                                    TTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAA                          <  SRR4302533.2929188/94‑1 (MQ=60)

                                                                    TTAATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAA                          <  SRR4302533.4803778/94‑1 (MQ=60)

                                                                      AATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATG                        <  SRR4302533.5395590/94‑1 (MQ=60)

                                                                       ATGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGA                       <  SRR4302533.1949948/94‑1 (MQ=60)

                                                                        TGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAG                      >  SRR4302533.1924982/1‑94 (MQ=60)

                                                                        TGTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAG                      >  SRR4302533.1365108/1‑94 (MQ=60)

                                                                         GTTAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGC                     <  SRR4302533.2241539/94‑1 (MQ=60)

                                                                           TAGCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAA                   <  SRR4302533.1833842/94‑1 (MQ=60)

                                                                             GCGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAAT                 >  SRR4302533.5920191/1‑94 (MQ=60)

                                                                              CGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACCGGCAGAAAAAATGGCGAATGAGCAAATT                >  SRR4302533.1016807/1‑94 (MQ=60)

                                                                              CGAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATT                <  SRR4302533.4105736/94‑1 (MQ=60)

                                                                               GAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTG               >  SRR4302533.5520315/1‑94 (MQ=60)

                                                                               GAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTG               <  SRR4302533.1585609/94‑1 (MQ=60)

                                                                               GAACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTG               <  SRR4302533.5326989/94‑1 (MQ=60)

                                                                                AACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGC              >  SRR4302533.5418883/1‑94 (MQ=60)

                                                                                AACTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGC              >  SRR4302533.2523875/1‑94 (MQ=60)

                                                                                  CTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTC            <  SRR4302533.1158127/94‑1 (MQ=60)

                                                                                  CTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTC            <  SRR4302533.6310383/94‑1 (MQ=60)

                                                                                  CTGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTC            <  SRR4302533.220130/94‑1 (MQ=60)

                                                                                   TGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCG           >  SRR4302533.3515233/1‑94 (MQ=60)

                                                                                   TGGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCG           <  SRR4302533.5523748/94‑1 (MQ=60)

                                                                                    GGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGT          >  SRR4302533.1497434/1‑94 (MQ=60)

                                                                                    GGAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGT          >  SRR4302533.4716287/1‑94 (MQ=60)

                                                                                     GAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTC         >  SRR4302533.1090082/1‑94 (MQ=60)

                                                                                     GAGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTC         <  SRR4302533.4400484/94‑1 (MQ=60)

                                                                                      AGACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCA        <  SRR4302533.5079963/94‑1 (MQ=60)

                                                                                        ACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGG      <  SRR4302533.4563059/94‑1 (MQ=60)

                                                                                        ACAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGG      >  SRR4302533.3044901/1‑94 (MQ=60)

                                                                                         CAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGT     >  SRR4302533.396017/1‑94 (MQ=60)

                                                                                         CAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGT     >  SRR4302533.6225680/1‑94 (MQ=60)

                                                                                         CAACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCCGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGT     >  SRR4302533.4456400/1‑94 (MQ=60)

                                                                                          AACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTT    >  SRR4302533.4437387/1‑94 (MQ=60)

                                                                                          AACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTT    <  SRR4302533.5505583/94‑1 (MQ=60)

                                                                                           ACGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTG   >  SRR4302533.2566281/1‑94 (MQ=60)

                                                                                            CGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGA  >  SRR4302533.4192473/1‑94 (MQ=60)

                                                                                            CGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGA  <  SRR4302533.4659340/94‑1 (MQ=60)

                                                                                            CGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGA  >  SRR4302533.2824369/1‑94 (MQ=60)

TTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCTCCTGTTAATGTTAGCGAACTGGAGACAATGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGA  >  NC_000913/4103549‑4103734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`