Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,106,773 A→G V120V (GTA→GTG)  sbp → periplasmic sulfate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,106,7730AG100.0% 755.5 / NA 233V120V (GTA→GTG) sbpperiplasmic sulfate‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (117/116);  total (117/116)

Only 100 of 240 total aligned reads displayed.

ATTGCGGAACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTACGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGCTCTGG  >  NC_000913/4106684‑4106862
                                                                                         |                                                                                         
aTTGCGGAACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGt                                                                                        >  2:4902567/1‑93 (MQ=255)
aTTGCGGAACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGt                                                                                        >  1:1319514/1‑93 (MQ=255)
 ttGCGGAACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTa                                                                                       >  2:2169949/1‑93 (MQ=255)
   gCGTAACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAg                                                                                     <  2:5693677/93‑1 (MQ=255)
   gCGGACCGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAg                                                                                     <  1:5668994/93‑1 (MQ=255)
    cGGAACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAgg                                                                                    >  2:6010112/1‑93 (MQ=255)
    cGGAACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAgg                                                                                    <  1:2242168/93‑1 (MQ=255)
     ggAACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAggg                                                                                   <  2:33433/93‑1 (MQ=255)
     ggAACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAggg                                                                                   <  1:4709666/93‑1 (MQ=255)
     ggAACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAggg                                                                                   <  1:5085143/93‑1 (MQ=255)
       aaCGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGaa                                                                                 >  2:5145611/1‑93 (MQ=255)
       aaCGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGaa                                                                                 <  2:6247139/93‑1 (MQ=255)
        aCGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGaaa                                                                                >  1:1793633/1‑93 (MQ=255)
          gcgggcggATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATc                                                                              <  2:5453407/93‑1 (MQ=255)
          gcgggcggATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATc                                                                              <  1:2461687/93‑1 (MQ=255)
            gggcggATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCg                                                                            >  1:2730236/1‑93 (MQ=255)
             ggcggATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGa                                                                           <  1:5412657/93‑1 (MQ=255)
              gcggATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCCCCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGaa                                                                          <  1:555511/93‑1 (MQ=255)
                ggATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGc                                                                        <  1:4862438/93‑1 (MQ=255)
                ggATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGc                                                                        >  2:3964684/1‑93 (MQ=255)
                 gATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCa                                                                       >  1:1945083/1‑93 (MQ=255)
                  aTTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAg                                                                      <  1:6111617/93‑1 (MQ=255)
                   ttGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGa                                                                     >  2:1202810/1‑93 (MQ=255)
                      aTAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATcc                                                                  <  1:4126547/93‑1 (MQ=255)
                       tAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCa                                                                 >  1:2999848/1‑93 (MQ=255)
                       tAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCa                                                                 <  2:3535391/93‑1 (MQ=255)
                         aaGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATg                                                               <  2:2364215/93‑1 (MQ=255)
                         aaGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATg                                                               <  1:4503761/93‑1 (MQ=255)
                          agagTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGa                                                              >  1:2534409/1‑93 (MQ=255)
                           gagTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGAc                                                             <  2:5195950/93‑1 (MQ=255)
                           gagTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGAc                                                             <  1:313103/93‑1 (MQ=255)
                             gTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTg                                                           <  2:4979364/93‑1 (MQ=255)
                              tGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTgg                                                          >  1:2137181/1‑93 (MQ=255)
                              tGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTgg                                                          <  2:2691970/93‑1 (MQ=255)
                               ggATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGa                                                         >  2:5215547/1‑93 (MQ=255)
                               ggATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGa                                                         <  1:2778994/93‑1 (MQ=255)
                                  tCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACg                                                      >  1:3638962/1‑93 (MQ=255)
                                     aaCGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATc                                                   <  2:4588038/93‑1 (MQ=255)
                                      aCGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGCTTTCCTGTTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCt                                                  >  1:4440144/1‑93 (MQ=255)
                                       cGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTg                                                 <  1:2603415/93‑1 (MQ=255)
                                        gTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGa                                                <  1:4278487/93‑1 (MQ=255)
                                          cTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGAtt                                              <  1:1251858/93‑1 (MQ=255)
                                           tGCCTGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTa                                             <  2:2568288/93‑1 (MQ=255)
                                           tGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTa                                             <  1:1859857/93‑1 (MQ=255)
                                           tGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTa                                             >  2:5865057/1‑93 (MQ=255)
                                           tGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTa                                             <  2:5523356/93‑1 (MQ=255)
                                           tGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTa                                             <  1:4370518/93‑1 (MQ=255)
                                            gCCGGATAATTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGCAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTaa                                            >  2:4500636/1‑93 (MQ=255)
                                            gCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTaa                                            >  2:4604708/1‑93 (MQ=255)
                                            gCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTaa                                            >  2:5722493/1‑93 (MQ=255)
                                              cGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAAc                                          <  2:3446105/93‑1 (MQ=255)
                                               ggATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAAcc                                         >  1:6182181/1‑93 (MQ=255)
                                               ggATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAAcc                                         >  1:5333480/1‑93 (MQ=255)
                                                gATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCg                                        >  2:3665105/1‑93 (MQ=255)
                                                gATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCg                                        >  2:468528/1‑93 (MQ=255)
                                                gATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCg                                        >  2:6144374/1‑93 (MQ=255)
                                                gATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCg                                        <  1:3278424/93‑1 (MQ=255)
                                                 aTAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCgg                                       >  2:2804134/1‑93 (MQ=255)
                                                 aTAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCgg                                       <  2:5903501/93‑1 (MQ=255)
                                                  tagctcctcACCGTCCACTTCCTCCATTGTTTCCCTGGTGCGTAAGGTAAAGCCAAAGCATACCCATGACTGTAACGATCTGATTAATCCggg                                      <  2:1898336/90‑1 (MQ=255)
                                                  tAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCggg                                      >  1:4602748/1‑93 (MQ=255)
                                                   aaCTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGgt                                     <  1:2834408/93‑1 (MQ=255)
                                                        cGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTc                                >  1:3721542/1‑93 (MQ=255)
                                                        cGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTc                                >  2:1551260/1‑93 (MQ=255)
                                                             cGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGa                           <  2:2209699/93‑1 (MQ=255)
                                                             cGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGa                           >  2:611739/1‑93 (MQ=255)
                                                              gTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGAt                          >  2:116528/1‑93 (MQ=255)
                                                               tACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATc                         <  2:4302422/93‑1 (MQ=255)
                                                               tACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATc                         >  2:1299386/1‑93 (MQ=255)
                                                               tACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGCTTCGGTGATc                         >  2:282772/1‑93 (MQ=255)
                                                                acacTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCa                        <  2:4221336/93‑1 (MQ=255)
                                                                acacTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCa                        >  2:3149047/1‑93 (MQ=255)
                                                                acacTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCa                        <  1:5733071/93‑1 (MQ=255)
                                                                acacTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCa                        <  1:733648/93‑1 (MQ=255)
                                                                 cacTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCAc                       <  2:1467846/93‑1 (MQ=255)
                                                                   cTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGc                     >  1:4966227/1‑93 (MQ=255)
                                                                    ttCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGcc                    <  1:5167460/93‑1 (MQ=255)
                                                                      ccaccaTTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTa                  <  1:1004741/93‑1 (MQ=255)
                                                                       caccaTTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTaa                 >  2:1961067/1‑93 (MQ=255)
                                                                       caccaTTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTaa                 <  1:4661007/93‑1 (MQ=255)
                                                                        accaTTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAAt                <  2:3015585/93‑1 (MQ=255)
                                                                        accaTTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAAt                >  2:212260/1‑93 (MQ=255)
                                                                        accaTTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCAATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAAt                >  1:3380879/1‑93 (MQ=255)
                                                                             tGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGaa           >  2:1779134/1‑93 (MQ=255)
                                                                             tGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGaa           >  1:2006067/1‑93 (MQ=255)
                                                                              gTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGaaa          >  1:6272135/1‑93 (MQ=255)
                                                                              gTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGaaa          <  2:5616950/93‑1 (MQ=255)
                                                                               ttttCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGaaaa         <  1:545565/93‑1 (MQ=255)
                                                                               ttttCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGaaaa         <  1:1202727/93‑1 (MQ=255)
                                                                                 ttCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGc       <  2:4057588/93‑1 (MQ=255)
                                                                                 ttCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGc       <  2:3761473/93‑1 (MQ=255)
                                                                                 ttCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGc       >  1:1633211/1‑93 (MQ=255)
                                                                                  tCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGct      >  1:1172710/1‑93 (MQ=255)
                                                                                   ccTGTTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGctc     <  1:974834/93‑1 (MQ=255)
                                                                                   ccTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGctc     <  2:633922/93‑1 (MQ=255)
                                                                                    cTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGctct    <  1:1955476/93‑1 (MQ=255)
                                                                                      ggTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGCTCtgg  >  2:3881816/1‑93 (MQ=255)
                                                                                      ggTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGCTCtgg  <  2:250460/93‑1 (MQ=255)
                                                                                      ggTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGCTCtgg  >  2:1039640/1‑93 (MQ=255)
                                                                                      ggTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGCTCtgg  <  1:58389/93‑1 (MQ=255)
                                                                                         |                                                                                         
ATTGCGGAACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTACGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGCTCTGG  >  NC_000913/4106684‑4106862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCAATTGCGGAACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTACGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGCTCTGGTGGC  >  NC_000913/4106680‑4106866
                                                                                             |                                                                                             
CGCAATTGCGGAACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTG                                                                                               >  SRR4302533.2806781/1‑94 (MQ=60)
CGCAATTGCGGAACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTG                                                                                               <  SRR4302533.4010346/94‑1 (MQ=60)
CGCAATTGCGGAACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTG                                                                                               >  SRR4302533.1706756/1‑94 (MQ=60)
 GCAATTGCGGAACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGC                                                                                              >  SRR4302533.4587034/1‑94 (MQ=60)
   AATTGCGGAACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGT                                                                                            >  SRR4302533.3429217/1‑94 (MQ=60)
   AATTGCGGAACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGT                                                                                            >  SRR4302533.1327433/1‑94 (MQ=60)
       GCGGACCGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGG                                                                                        <  SRR4302533.5703238/94‑1 (MQ=60)
        CGGAACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGG                                                                                       <  SRR4302533.2256026/94‑1 (MQ=60)
         GGAACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGA                                                                                      <  SRR4302533.4738210/94‑1 (MQ=60)
         GGAACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGA                                                                                      <  SRR4302533.5115873/94‑1 (MQ=60)
           AACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAA                                                                                    <  SRR4302533.4398909/94‑1 (MQ=60)
           AACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAA                                                                                    >  SRR4302533.1804549/1‑94 (MQ=60)
            ACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAAT                                                                                   <  SRR4302533.5315455/94‑1 (MQ=60)
              GCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCC                                                                                 <  SRR4302533.2476873/94‑1 (MQ=60)
               CGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCG                                                                                >  SRR4302533.5497930/1‑94 (MQ=60)
               CGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCG                                                                                >  SRR4302533.2746982/1‑94 (MQ=60)
                GGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGA                                                                               >  SRR4302533.5371531/1‑94 (MQ=60)
                 GGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAA                                                                              <  SRR4302533.5445204/94‑1 (MQ=60)
                  GCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCCCCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAG                                                                             <  SRR4302533.558807/94‑1 (MQ=60)
                    GGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCA                                                                           >  SRR4302533.546639/1‑94 (MQ=60)
                    GGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCA                                                                           <  SRR4302533.4891909/94‑1 (MQ=60)
                    GGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCA                                                                           >  SRR4302533.1956946/1‑94 (MQ=60)
                      ATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGA                                                                         <  SRR4302533.6148507/94‑1 (MQ=60)
                       TTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGAT                                                                        <  SRR4302533.5969045/94‑1 (MQ=60)
                        TGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATC                                                                       >  SRR4302533.1246220/1‑94 (MQ=60)
                         GATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCC                                                                      <  SRR4302533.446148/94‑1 (MQ=60)
                          ATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCA                                                                     >  SRR4302533.1967289/1‑94 (MQ=60)
                          ATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCA                                                                     >  SRR4302533.3018194/1‑94 (MQ=60)
                          ATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCA                                                                     <  SRR4302533.4151597/94‑1 (MQ=60)
                            AAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATG                                                                   <  SRR4302533.4603564/94‑1 (MQ=60)
                             AAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGA                                                                  <  SRR4302533.4531044/94‑1 (MQ=60)
                             AAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGA                                                                  >  SRR4302533.5423806/1‑94 (MQ=60)
                             AAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGA                                                                  >  SRR4302533.2550010/1‑94 (MQ=60)
                              AGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGAC                                                                 >  SRR4302533.4864815/1‑94 (MQ=60)
                               GAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACT                                                                <  SRR4302533.315014/94‑1 (MQ=60)
                                AGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGTTGCGTAAGGGAAATCCGAAGCAGATCCATGACTG                                                               <  SRR4302533.260020/94‑1 (MQ=60)
                                AGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTG                                                               >  SRR4302533.578238/1‑94 (MQ=60)
                                 GTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGG                                                              <  SRR4302533.1993788/94‑1 (MQ=60)
                                 GTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGG                                                              >  SRR4302533.2150361/1‑94 (MQ=60)
                                  TGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGA                                                             >  SRR4302533.1478281/1‑94 (MQ=60)
                                  TGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGA                                                             <  SRR4302533.6179031/94‑1 (MQ=60)
                                   GGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAA                                                            >  SRR4302533.4633342/1‑94 (MQ=60)
                                   GGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAA                                                            <  SRR4302533.2796064/94‑1 (MQ=60)
                                    GATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAAC                                                           <  SRR4302533.4743144/94‑1 (MQ=60)
                                    GATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAAC                                                           <  SRR4302533.5824019/94‑1 (MQ=60)
                                     ATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACG                                                          >  SRR4302533.3661337/1‑94 (MQ=60)
                                         AACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGCTTTCCTGTTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCT                                                      >  SRR4302533.4466986/1‑94 (MQ=60)
                                           CGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGA                                                    <  SRR4302533.2619409/94‑1 (MQ=60)
                                            GTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGAT                                                   >  SRR4302533.518055/1‑94 (MQ=60)
                                            GTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGAT                                                   <  SRR4302533.4304217/94‑1 (MQ=60)
                                             TCTGCCTGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATT                                                  <  SRR4302533.4035734/94‑1 (MQ=60)
                                             TCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATT                                                  <  SRR4302533.25138/94‑1 (MQ=60)
                                              CTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTA                                                 >  SRR4302533.4518093/1‑94 (MQ=60)
                                              CTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTA                                                 <  SRR4302533.1259337/94‑1 (MQ=60)
                                              CTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTA                                                 <  SRR4302533.1023635/94‑1 (MQ=60)
                                               TGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAA                                                <  SRR4302533.4396902/94‑1 (MQ=60)
                                               TGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAA                                                <  SRR4302533.1871200/94‑1 (MQ=60)
                                               TGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAA                                                <  SRR4302533.2896685/94‑1 (MQ=60)
                                                 CCGGTTAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAAC                                              <  SRR4302533.1970011/94‑1 (MQ=60)
                                                  CGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACC                                             >  SRR4302533.5365447/1‑94 (MQ=60)
                                                  CGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACC                                             >  SRR4302533.6219304/1‑94 (MQ=60)
                                                   GGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCG                                            >  SRR4302533.4988723/1‑94 (MQ=60)
                                                   GGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCG                                            >  SRR4302533.2445081/1‑94 (MQ=60)
                                                    GATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGG                                           <  SRR4302533.3298667/94‑1 (MQ=60)
                                                    GATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGG                                           <  SRR4302533.694225/94‑1 (MQ=60)
                                                     ATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGG                                          >  SRR4302533.4630631/1‑94 (MQ=60)
                                                     ATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGG                                          >  SRR4302533.318532/1‑94 (MQ=60)
                                                      TAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGCAGCAGATCCATGACTTGAACGATCTGATTAAACCGGGT                                         >  SRR4302533.1911142/1‑94 (MQ=60)
                                                       AACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTG                                        <  SRR4302533.2851855/94‑1 (MQ=60)
                                                          TCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTT                                     >  SRR4302533.6277377/1‑94 (MQ=60)
                                                           CCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTC                                    >  SRR4302533.3744382/1‑94 (MQ=60)
                                                           CCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGCGTTTC                                    >  SRR4302533.2819904/1‑94 (MQ=60)
                                                            CGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCG                                   <  SRR4302533.765301/94‑1 (MQ=60)
                                                              CACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGT                                 <  SRR4302533.6210743/94‑1 (MQ=60)
                                                              CACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGT                                 >  SRR4302533.5734771/1‑94 (MQ=60)
                                                               CCCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTG                                <  SRR4302533.3733008/94‑1 (MQ=60)
                                                                CCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGA                               <  SRR4302533.2189682/94‑1 (MQ=60)
                                                                 CGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGAT                              <  SRR4302533.3558284/94‑1 (MQ=60)
                                                                  GTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATC                             >  SRR4302533.1268133/1‑94 (MQ=60)
                                                                   TACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCA                            <  SRR4302533.457514/94‑1 (MQ=60)
                                                                   TACACTTCCACCATTGGTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGGGTTTCGGTGATCA                            <  SRR4302533.310240/94‑1 (MQ=60)
                                                                    ACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCAC                           <  SRR4302533.5767683/94‑1 (MQ=60)
                                                                    ACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCAC                           <  SRR4302533.737964/94‑1 (MQ=60)
                                                                     CACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACG                          <  SRR4302533.339746/94‑1 (MQ=60)
                                                                     CACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACG                          >  SRR4302533.933114/1‑94 (MQ=60)
                                                                      ACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGC                         >  SRR4302533.4996334/1‑94 (MQ=60)
                                                                       CTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCC                        <  SRR4302533.5607632/94‑1 (MQ=60)
                                                                       CTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCC                        >  SRR4302533.2614536/1‑94 (MQ=60)
                                                                       CTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCC                        >  SRR4302533.2317717/1‑94 (MQ=60)
                                                                       CTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCC                        >  SRR4302533.1875681/1‑94 (MQ=60)
                                                                       CTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCC                        >  SRR4302533.1740068/1‑94 (MQ=60)
                                                                        TTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCT                       >  SRR4302533.2791677/1‑94 (MQ=60)
                                                                        TTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCT                       <  SRR4302533.5090162/94‑1 (MQ=60)
                                                                        TTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCT                       <  SRR4302533.5198656/94‑1 (MQ=60)
                                                                         TCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTA                      >  SRR4302533.1200239/1‑94 (MQ=60)
                                                                          CCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAA                     <  SRR4302533.1010618/94‑1 (MQ=60)
                                                                           CACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAAT                    <  SRR4302533.4689251/94‑1 (MQ=60)
                                                                           CACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCAATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAAT                    >  SRR4302533.3401749/1‑94 (MQ=60)
                                                                            ACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATC                   >  SRR4302533.1477676/1‑94 (MQ=60)
                                                                             CCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGCGGTTCGGTGATCACGCCTAATCC                  >  SRR4302533.3018294/1‑94 (MQ=60)
                                                                              CATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCG                 >  SRR4302533.1257712/1‑94 (MQ=60)
                                                                                TTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAA               >  SRR4302533.2018422/1‑94 (MQ=60)
                                                                                TTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAA               >  SRR4302533.2718068/1‑94 (MQ=60)
                                                                                TTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAA               >  SRR4302533.4068962/1‑94 (MQ=60)
                                                                                 TGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAA              >  SRR4302533.6309600/1‑94 (MQ=60)
                                                                                 TGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAA              >  SRR4302533.4396363/1‑94 (MQ=60)
                                                                                   TTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAG            >  SRR4302533.5548207/1‑94 (MQ=60)
                                                                                   TTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAG            <  SRR4302533.548806/94‑1 (MQ=60)
                                                                                   TTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAG            >  SRR4302533.2831003/1‑94 (MQ=60)
                                                                                   TTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAG            <  SRR4302533.1209955/94‑1 (MQ=60)
                                                                                    TTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGC           >  SRR4302533.1643102/1‑94 (MQ=60)
                                                                                     TTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGCT          <  SRR4302533.4702172/94‑1 (MQ=60)
                                                                                     TTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGCT          >  SRR4302533.1179749/1‑94 (MQ=60)
                                                                                      TCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGCTC         >  SRR4302533.2048745/1‑94 (MQ=60)
                                                                                      TCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGCTC         <  SRR4302533.5050990/94‑1 (MQ=60)
                                                                                       CCTGTTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGCTCT        <  SRR4302533.980556/94‑1 (MQ=60)
                                                                                       CCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGCTCT        >  SRR4302533.1810920/1‑94 (MQ=60)
                                                                                       CCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGCTCT        <  SRR4302533.1000054/94‑1 (MQ=60)
                                                                                        CTGGTGCGTAAGTGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGCTCTG       <  SRR4302533.4192488/94‑1 (MQ=60)
                                                                                        CTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGCTCTG       <  SRR4302533.1967390/94‑1 (MQ=60)
                                                                                          GGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGCTCTGGT     <  SRR4302533.58774/94‑1 (MQ=60)
                                                                                          GGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGCTCTGGT     >  SRR4302533.6228808/1‑94 (MQ=60)
                                                                                          GGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGCTCTGGT     >  SRR4302533.2903894/1‑94 (MQ=60)
                                                                                             GCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGCTCTGGTGGC  <  SRR4302533.4333576/94‑1 (MQ=60)
                                                                                             GCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGCTCTGGTGGC  <  SRR4302533.4628360/94‑1 (MQ=60)
                                                                                             |                                                                                             
CGCAATTGCGGAACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTACGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGCTCTGGTGGC  >  NC_000913/4106680‑4106866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑