Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,175,949	+G	intergenic (+82/‑76)	nusG → / → rplK	NusG/50S ribosomal subunit protein L11

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,175,947	1	.	G	99.0%	626.1 / ‑1.3	195	intergenic (+80/‑78)	nusG/rplK	NusG/50S ribosomal subunit protein L11
Reads supporting (aligned to +/- strand): ref base . (0/2); new base G (106/87); total (106/89)
Fisher's exact test for biased strand distribution p-value = 2.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 215 total aligned reads displayed.

TGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGA  >  NC_000913/4175855‑4176034

tGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAt                                                                                          <  1:372950/93‑1 (MQ=255)

    aaaGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑gggc                                                                                     >  2:4940622/1‑91 (MQ=255)

     aaGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑gggcc                                                                                    >  1:2820616/1‑90 (MQ=255)

      aGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑gggcct                                                                                   >  1:5819600/1‑89 (MQ=255)

      aGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑gggcct                                                                                   <  1:837110/93‑5 (MQ=255)

      aGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑gggcct                                                                                   <  2:3816671/93‑5 (MQ=255)

         cTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGc                                                                                 >  1:4913935/1‑93 (MQ=255)

         cTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGc                                                                                 >  2:5759198/1‑93 (MQ=255)

          tAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGcc                                                                                >  1:1199935/1‑93 (MQ=255)

          tAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGcc                                                                                >  1:5706360/1‑93 (MQ=255)

          tAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGcc                                                                                >  2:2373100/1‑93 (MQ=255)

            aCCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCg                                                                              >  1:5242544/1‑93 (MQ=255)

            aCCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCg                                                                              >  2:1875190/1‑93 (MQ=255)

              ccAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTa                                                                            >  2:2271217/1‑93 (MQ=255)

               cAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCGCGCaa                                                                           >  1:6016937/1‑93 (MQ=255)

               cAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTaa                                                                           >  1:4738346/1‑93 (MQ=255)

                aGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTaaa                                                                          <  1:4522048/93‑1 (MQ=255)

                   gATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACg                                                                       >  2:3371686/1‑93 (MQ=255)

                   gATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACg                                                                       <  2:1749514/93‑1 (MQ=255)

                    aTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGa                                                                      >  2:1593979/1‑93 (MQ=255)

                     tCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGAt                                                                     >  1:1000279/1‑93 (MQ=255)

                      cAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGAtt                                                                    <  2:4416258/93‑1 (MQ=255)

                      cAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGAtt                                                                    <  1:3865109/93‑1 (MQ=255)

                      cAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGAtt                                                                    <  1:935252/93‑1 (MQ=255)

                      cAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGAtt                                                                    <  2:1324801/93‑1 (MQ=255)

                       aaaaaaGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGAttt                                                                   >  1:2320361/1‑93 (MQ=255)

                        aaaaaGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGAtttt                                                                  >  1:3667471/1‑93 (MQ=255)

                        aaaaaGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGAtttt                                                                  <  2:4623310/93‑1 (MQ=255)

                        aaaaaGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGAtttt                                                                  <  2:6134931/93‑1 (MQ=255)

                           aaGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTta                                                               <  1:6112644/93‑1 (MQ=255)

                             gcggcgATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTtata                                                             <  2:841997/93‑1 (MQ=255)

                               ggcgATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATc                                                           >  2:952014/1‑93 (MQ=255)

                               ggcgATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATc                                                           <  1:1667113/93‑1 (MQ=255)

                               ggcgATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATc                                                           >  2:2850703/1‑93 (MQ=255)

                               ggcgATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATc                                                           <  1:2611829/93‑1 (MQ=255)

                                gcgATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCa                                                          >  2:2732698/1‑93 (MQ=255)

                                 cgATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCAc                                                         <  1:794930/93‑1 (MQ=255)

                                 cgATTTAATCGTTGCACAAGGAGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCAc                                                         >  1:1779014/1‑93 (MQ=255)

                                  gATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACg                                                        <  2:1345509/93‑1 (MQ=255)

                                   aTTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACgg                                                       <  2:3857308/93‑1 (MQ=255)

                                   aTTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACgg                                                       >  1:4626588/1‑93 (MQ=255)

                                   aTTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACgg                                                       >  2:5169715/1‑93 (MQ=255)

                                   aTTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACgg                                                       >  1:4106801/1‑93 (MQ=255)

                                   aTTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACgg                                                       >  1:1515884/1‑93 (MQ=255)

                                    tttAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACggg                                                      >  1:2267582/1‑93 (MQ=255)

                                     ttAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACgggg                                                     <  1:1248772/93‑1 (MQ=255)

                                      tAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTGATATCGCGGGGa                                                    <  1:3405500/93‑1 (MQ=255)

                                        aTCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGc                                                  <  2:3538733/93‑1 (MQ=255)

                                          cGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCct                                                <  2:1467239/93‑1 (MQ=255)

                                           gTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCctc                                               >  1:2026441/1‑93 (MQ=255)

                                            ttGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCctct                                              <  2:3323381/93‑1 (MQ=255)

                                              gCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCGTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCa                                            <  1:4840961/93‑1 (MQ=255)

                                              gCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCa                                            <  1:1560487/93‑1 (MQ=255)

                                               cacaAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCag                                           >  1:5906051/1‑93 (MQ=255)

                                               cacaAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCag                                           <  2:2342098/93‑1 (MQ=255)

                                                acaAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCaga                                          <  2:5993229/93‑1 (MQ=255)

                                                acaAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCaga                                          >  1:5257342/1‑93 (MQ=255)

                                                 caAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCagag                                         <  2:2488547/93‑1 (MQ=255)

                                                  aaGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAgg                                        <  2:3572741/93‑1 (MQ=255)

                                                  aaGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAgg                                        >  2:3214232/1‑93 (MQ=255)

                                                   aGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGc                                       >  2:4265375/1‑93 (MQ=255)

                                                   aGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGc                                       >  2:5622967/1‑93 (MQ=255)

                                                       gTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGtta                                   <  2:1950262/93‑1 (MQ=255)

                                                       gTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGtta                                   >  2:2148396/1‑93 (MQ=255)

                                                       gTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGtta                                   >  1:4310692/1‑93 (MQ=255)

                                                        tGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGttat                                  >  2:5420391/1‑93 (MQ=255)

                                                           gaTTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTAc                               >  1:3537107/1‑93 (MQ=255)

                                                            aTTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTAcc                              <  1:1333233/93‑1 (MQ=255)

                                                             ttGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTAccc                             <  2:2084171/93‑1 (MQ=255)

                                                              tGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCa                            >  2:367650/1‑93 (MQ=255)

                                                              tGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCa                            <  2:5113506/93‑1 (MQ=255)

                                                              tGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCa                            <  1:2490351/93‑1 (MQ=255)

                                                               ggAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCaa                           <  1:6024436/93‑1 (MQ=255)

                                                               ggAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCaa                           >  1:564708/1‑93 (MQ=255)

                                                               ggAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCaa                           >  2:3696230/1‑93 (MQ=255)

                                                                gAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAAc                          >  1:1073939/1‑93 (MQ=255)

                                                                gAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAAc                          >  1:315402/1‑93 (MQ=255)

                                                                 aaTATAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACt                         >  2:2994212/1‑93 (MQ=255)

                                                                 aaTACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACt                         >  1:967459/1‑93 (MQ=255)

                                                                   tgcAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTg                       <  1:6294697/91‑1 (MQ=255)

                                                                    aCAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGa                      <  2:4528025/93‑1 (MQ=255)

                                                                    aCAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGa                      <  1:4109890/93‑1 (MQ=255)

                                                                     cAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAg                     <  2:684912/93‑1 (MQ=255)

                                                                     cAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAg                     <  1:4057074/93‑1 (MQ=255)

                                                                     cAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAg                     <  2:547013/93‑1 (MQ=255)

                                                                          tCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAAtt                <  1:3563871/93‑1 (MQ=255)

                                                                          tCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAAtt                >  2:5368155/1‑93 (MQ=255)

                                                                           cgcgCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAAttt               <  1:4042494/93‑1 (MQ=255)

                                                                            gcgcCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTta              >  2:5898106/1‑93 (MQ=255)

                                                                             cgcCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTtat             >  2:479400/1‑93 (MQ=255)

                                                                              gcCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTtata            >  1:3216243/1‑93 (MQ=255)

                                                                              gcCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTtata            >  1:1741018/1‑93 (MQ=255)

                                                                               ccTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATaa           <  1:3779378/93‑1 (MQ=255)

                                                                                cTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAAt          >  1:2060810/1‑93 (MQ=255)

                                                                                cTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAAt          >  1:6095577/1‑93 (MQ=255)

                                                                                 ttttGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATg         >  1:3799306/1‑93 (MQ=255)

                                                                                 ttttGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATg         <  1:5394552/93‑1 (MQ=255)

                                                                                      tttttATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTaa    >  2:1313025/1‑93 (MQ=255)

                                                                                      tttttATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTaa    <  1:5108855/93‑1 (MQ=255)

                                                                                        tttATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGa  >  2:4040482/1‑93 (MQ=255)

TGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGA  >  NC_000913/4175855‑4176034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTACAA  >  NC_000913/4175857‑4176042

AAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GGG                                                                                                <  SRR4302533.1050917/94‑1 (MQ=60)

AAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GGG                                                                                                >  SRR4302533.5283349/1‑94 (MQ=60)

AAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GGG                                                                                                <  SRR4302533.1789353/94‑1 (MQ=60)

AAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GGG                                                                                                <  SRR4302533.1653333/94‑1 (MQ=60)

 AAAGGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GGGC                                                                                               <  SRR4302533.2298238/94‑1 (MQ=60)

  AAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GGgcc                                                                                              >  SRR4302533.2837973/1‑91 (MQ=60)

   AAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑T                                                                                            >  SRR4302533.5854756/1‑94 (MQ=60)

    AGCCTAACCCAGCGATCAAAAACGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TT                                                                                           <  SRR4302533.6147615/94‑1 (MQ=60)

    AGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TT                                                                                           <  SRR4302533.3012752/94‑1 (MQ=60)

    AGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TT                                                                                           <  SRR4302533.2338555/94‑1 (MQ=60)

    AGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TT                                                                                           <  SRR4302533.842014/94‑1 (MQ=60)

     GCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTG                                                                                          <  SRR4302533.4926323/94‑1 (MQ=60)

      CCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGC                                                                                         >  SRR4302533.4943699/1‑94 (MQ=60)

       CTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCC                                                                                        >  SRR4302533.1207141/1‑94 (MQ=60)

       CTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCC                                                                                        >  SRR4302533.4614608/1‑94 (MQ=60)

       CTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCC                                                                                        >  SRR4302533.5740827/1‑94 (MQ=60)

        TAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCC                                                                                       >  SRR4302533.1244783/1‑94 (MQ=60)

         AACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCG                                                                                      >  SRR4302533.5274056/1‑94 (MQ=60)

          ACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGT                                                                                     <  SRR4302533.3579239/94‑1 (MQ=60)

           CCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTA                                                                                    <  SRR4302533.3816039/94‑1 (MQ=60)

            CCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAA                                                                                   >  SRR4302533.961217/1‑94 (MQ=60)

            CCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAA                                                                                   >  SRR4302533.4767076/1‑94 (MQ=60)

            CCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAA                                                                                   >  SRR4302533.5780059/1‑94 (MQ=60)

            CCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GGgccttgcgcgcaa                                                                                    >  SRR4302533.6053394/1‑81 (MQ=60)

              AGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAA                                                                                 <  SRR4302533.4549426/94‑1 (MQ=60)

                CGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACG                                                                               >  SRR4302533.778767/1‑94 (MQ=60)

                  ATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGAT                                                                             >  SRR4302533.1006116/1‑94 (MQ=60)

                  ATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGAT                                                                             >  SRR4302533.246496/1‑94 (MQ=60)

                    CAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTT                                                                           <  SRR4302533.3888704/94‑1 (MQ=60)

                    CAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTT                                                                           <  SRR4302533.940742/94‑1 (MQ=60)

                    CAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTT                                                                           >  SRR4302533.2334726/1‑94 (MQ=60)

                     AAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTT                                                                          >  SRR4302533.3690013/1‑94 (MQ=60)

                        AAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTA                                                                       >  SRR4302533.4917979/1‑94 (MQ=60)

                         AAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTAT                                                                      <  SRR4302533.6149536/94‑1 (MQ=60)

                         AAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTAT                                                                      <  SRR4302533.523420/94‑1 (MQ=60)

                         AAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTAT                                                                      <  SRR4302533.829708/94‑1 (MQ=60)

                            CGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATC                                                                   >  SRR4302533.2082044/1‑94 (MQ=60)

                             GGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCA                                                                  <  SRR4302533.1677184/94‑1 (MQ=60)

                             GGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCA                                                                  <  SRR4302533.2627860/94‑1 (MQ=60)

                              GCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCAC                                                                 <  SRR4302533.3938804/94‑1 (MQ=60)

                              GCGATTTAATCGTTGCACAAGGAGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCAC                                                                 >  SRR4302533.1789845/1‑94 (MQ=60)

                               CGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACG                                                                <  SRR4302533.2069559/94‑1 (MQ=60)

                               CGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACG                                                                <  SRR4302533.3749544/94‑1 (MQ=60)

                               CGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACG                                                                <  SRR4302533.799607/94‑1 (MQ=60)

                                GATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGG                                                               >  SRR4302533.4654628/1‑94 (MQ=60)

                                GATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGG                                                               >  SRR4302533.4131773/1‑94 (MQ=60)

                                GATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGG                                                               >  SRR4302533.1525012/1‑94 (MQ=60)

                                 ATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGG                                                              >  SRR4302533.4126971/1‑94 (MQ=60)

                                 ATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGG                                                              >  SRR4302533.2281608/1‑94 (MQ=60)

                                  TTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGG                                                             >  SRR4302533.6058998/1‑94 (MQ=60)

                                   TTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGA                                                            <  SRR4302533.1256232/94‑1 (MQ=60)

                                    TAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAG                                                           >  SRR4302533.2598558/1‑94 (MQ=60)

                                    TAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTGATATCGCGGGGAG                                                           <  SRR4302533.3426533/94‑1 (MQ=60)

                                      ATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCC                                                         <  SRR4302533.1394219/94‑1 (MQ=60)

                                       TCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCT                                                        <  SRR4302533.5867457/94‑1 (MQ=60)

                                        CGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTC                                                       >  SRR4302533.3630637/1‑94 (MQ=60)

                                        CGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTC                                                       >  SRR4302533.1622604/1‑94 (MQ=60)

                                        CGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTC                                                       >  SRR4302533.2038952/1‑94 (MQ=60)

                                            GCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCG‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAG                                                   <  SRR4302533.4870303/94‑1 (MQ=60)

                                            GCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAG                                                   <  SRR4302533.1569885/94‑1 (MQ=60)

                                            GCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAG                                                   >  SRR4302533.5941716/1‑94 (MQ=60)

                                            GCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAG                                                   >  SRR4302533.3405990/1‑94 (MQ=60)

                                             CACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGA                                                  >  SRR4302533.4071753/1‑94 (MQ=60)

                                             CACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGA                                                  >  SRR4302533.5288913/1‑94 (MQ=60)

                                              ACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAG                                                 <  SRR4302533.4756291/94‑1 (MQ=60)

                                              ACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAG                                                 <  SRR4302533.5072129/94‑1 (MQ=60)

                                              ACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAG                                                 >  SRR4302533.6208391/1‑94 (MQ=60)

                                                AAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGC                                               <  SRR4302533.4726165/94‑1 (MQ=60)

                                                AAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGC                                               <  SRR4302533.4132264/94‑1 (MQ=60)

                                                    CGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTA                                           >  SRR4302533.4196401/1‑94 (MQ=60)

                                                    CGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTA                                           >  SRR4302533.4336641/1‑94 (MQ=60)

                                                     GTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTAT                                          >  SRR4302533.5389522/1‑94 (MQ=60)

                                                      TGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATT                                         >  SRR4302533.902780/1‑94 (MQ=60)

                                                        AGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTAC                                       >  SRR4302533.3558907/1‑94 (MQ=60)

                                                          ATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCC                                     <  SRR4302533.6127080/94‑1 (MQ=60)

                                                          ATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCC                                     <  SRR4302533.1341236/94‑1 (MQ=60)

                                                           TTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCA                                    <  SRR4302533.267812/94‑1 (MQ=60)

                                                           TTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCA                                    >  SRR4302533.3857930/1‑94 (MQ=60)

                                                            TGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAA                                   <  SRR4302533.2505689/94‑1 (MQ=60)

                                                            TGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAA                                   >  SRR4302533.568054/1‑94 (MQ=60)

                                                            TGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAA                                   >  SRR4302533.568437/1‑94 (MQ=60)

                                                             GGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAAC                                  >  SRR4302533.3763379/1‑94 (MQ=60)

                                                             GGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAAC                                  >  SRR4302533.1080270/1‑94 (MQ=60)

                                                             GGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAAC                                  >  SRR4302533.317327/1‑94 (MQ=60)

                                                             GGAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAAC                                  <  SRR4302533.6060943/94‑1 (MQ=60)

                                                              GAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACT                                 >  SRR4302533.5609320/1‑94 (MQ=60)

                                                              GAATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACT                                 >  SRR4302533.973133/1‑94 (MQ=60)

                                                                ATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTG                               >  SRR4302533.10924/1‑94 (MQ=60)

                                                                ATACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTG                               >  SRR4302533.3929071/1‑94 (MQ=60)

                                                                 TGCAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGA                              <  SRR4302533.6332219/94‑1 (MQ=60)

                                                                  ACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAG                             <  SRR4302533.3553069/94‑1 (MQ=60)

                                                                  ACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAG                             <  SRR4302533.3553070/94‑1 (MQ=60)

                                                                  ACAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAG                             <  SRR4302533.4134874/94‑1 (MQ=60)

                                                                   CAATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGG                            <  SRR4302533.4081771/94‑1 (MQ=60)

                                                                     ATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAA                          >  SRR4302533.1326645/1‑94 (MQ=60)

                                                                     ATTTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAA                          >  SRR4302533.5403808/1‑94 (MQ=60)

                                                                       TTCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATT                        <  SRR4302533.6079685/94‑1 (MQ=60)

                                                                        TCGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTT                       <  SRR4302533.3585831/94‑1 (MQ=60)

                                                                         CGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGTGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATCTA                      >  SRR4302533.3450440/1‑94 (MQ=60)

                                                                         CGCGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTA                      <  SRR4302533.4067099/94‑1 (MQ=60)

                                                                         CGCGCCTTTCGTTTTTAT‑GTCCCTTTG‑CCGTAAAACGATTTTTGATATCACGGGTCGCCTCTCAGAGGCGTTATTACCCAACTTTAGGAATTTA                      <  SRR4302533.4198879/94‑1 (MQ=48)

                                                                           CGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATA                    <  SRR4302533.3217604/94‑1 (MQ=60)

                                                                           CGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATA                    >  SRR4302533.1751574/1‑94 (MQ=60)

                                                                           CGCCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATA                    >  SRR4302533.3236072/1‑94 (MQ=60)

                                                                             CCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAAT                  >  SRR4302533.2073521/1‑94 (MQ=60)

                                                                             CCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAAT                  <  SRR4302533.3802531/94‑1 (MQ=60)

                                                                             CCTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAAT                  >  SRR4302533.6132400/1‑94 (MQ=60)

                                                                              CTTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATG                 >  SRR4302533.3822565/1‑94 (MQ=60)

                                                                               TTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGG                <  SRR4302533.5426968/94‑1 (MQ=60)

                                                                               TTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGG                <  SRR4302533.2037323/94‑1 (MQ=60)

                                                                               TTTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGG                <  SRR4302533.1715567/94‑1 (MQ=60)

                                                                                TTTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGC               <  SRR4302533.4358924/94‑1 (MQ=60)

                                                                                 TTGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCT              <  SRR4302533.4788627/94‑1 (MQ=60)

                                                                                  TGTTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTA             >  SRR4302533.6035388/1‑94 (MQ=60)

                                                                                    TTTTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAG           <  SRR4302533.5139726/94‑1 (MQ=60)

                                                                                      TTTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAA         >  SRR4302533.903217/1‑94 (MQ=60)

                                                                                       TTATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAA        <  SRR4302533.3333266/94‑1 (MQ=60)

                                                                                        TATGGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAG       >  SRR4302533.5234010/1‑94 (MQ=60)

                                                                                          tgGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTA     >  SRR4302533.357854/3‑94 (MQ=60)

                                                                                          tgGGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTA     >  SRR4302533.1372167/3‑94 (MQ=60)

                                                                                          G‑GGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTAC    <  SRR4302533.2811830/94‑1 (MQ=60)

                                                                                          G‑GGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTAC    <  SRR4302533.1545133/94‑1 (MQ=60)

                                                                                          G‑GGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTAC    >  SRR4302533.496974/1‑94 (MQ=60)

                                                                                            GGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTACA   >  SRR4302533.6110428/1‑94 (MQ=60)

                                                                                            GGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTACA   <  SRR4302533.3891254/94‑1 (MQ=60)

                                                                                            GGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTACA   <  SRR4302533.5232325/94‑1 (MQ=60)

                                                                                            GGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTACA   <  SRR4302533.4698311/94‑1 (MQ=60)

                                                                                             GCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTACAA  >  SRR4302533.430162/1‑94 (MQ=60)

                                                                                             GCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTACAA  <  SRR4302533.1166188/94‑1 (MQ=60)

AAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GGCC‑‑TTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTACAA  >  NC_000913/4175857‑4176042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`