Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,205,438 T→C intergenic (‑328/‑287) purH ← / → rrsE phosphoribosylaminoimidazolecarboxamideformyltra nsferase/16S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,205,4380TC100.0% 534.3 / NA 170intergenic (‑328/‑287)purH/rrsEphosphoribosylaminoimidazolecarboxamideformyltra nsferase/16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (97/73);  total (97/73)

Only 100 of 179 total aligned reads displayed.

TAAAGCGGGCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAG  >  NC_000913/4205348‑4205512
                                                                                          |                                                                          
 aaaGCGGGCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCa                                                                         >  2:442313/1‑93 (MQ=255)
  aaGCGGGCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCAt                                                                        >  1:4852183/1‑93 (MQ=255)
  aaGCGGGCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCAt                                                                        <  1:5631518/93‑1 (MQ=255)
   aGCGGGCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATc                                                                       >  2:5786467/1‑93 (MQ=255)
    gCGGGCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCg                                                                      >  1:1811781/1‑93 (MQ=255)
     cGGGCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGa                                                                     >  2:2494025/1‑93 (MQ=255)
       ggCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGaca                                                                   >  1:92206/1‑93 (MQ=255)
        gCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGCGAACTCCCTATAATGCGCCCCCATCGacac                                                                  >  1:3420215/1‑93 (MQ=255)
        gCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGacac                                                                  >  2:4848683/1‑93 (MQ=255)
        gCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGacac                                                                  <  1:1648495/93‑1 (MQ=255)
        gCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGacac                                                                  <  1:3562654/93‑1 (MQ=255)
        gCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGacac                                                                  <  2:1026808/93‑1 (MQ=255)
          ttttGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACAcgg                                                                >  2:1759986/1‑93 (MQ=255)
          ttttGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACAcgg                                                                >  2:1621134/1‑93 (MQ=255)
          ttttGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACAcgg                                                                >  2:2174708/1‑93 (MQ=255)
          ttttGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACAcgg                                                                >  2:730668/1‑93 (MQ=255)
           tttGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGACCTCCCTATAATGCGCCCCCATCGACAcggc                                                               <  1:5549703/93‑1 (MQ=255)
           tttGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACAcggc                                                               >  1:5159658/1‑93 (MQ=255)
           tttGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACAcggc                                                               <  2:1406430/93‑1 (MQ=255)
             tGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACAcggcgg                                                             >  1:1518193/1‑93 (MQ=255)
              gCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACAcggcggt                                                            >  2:1239331/1‑92 (MQ=255)
                tGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGAtg                                                          >  2:4835552/1‑93 (MQ=255)
                tGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATAGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGAtg                                                          >  1:3384955/1‑93 (MQ=255)
                  gggAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGAtgtg                                                        >  2:4998759/1‑93 (MQ=255)
                   ggAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGa                                                       >  2:3487637/1‑93 (MQ=255)
                    gAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGaa                                                      >  2:3298306/1‑93 (MQ=255)
                      aTGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGGAGTGAATc                                                    >  2:4342512/1‑93 (MQ=255)
                      aTGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATc                                                    >  2:3640970/1‑93 (MQ=255)
                       tGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCa                                                   <  1:2885511/93‑1 (MQ=255)
                        gcagcaGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCAc                                                  <  1:4341287/93‑1 (MQ=255)
                         cagcagTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACt                                                 <  1:3826655/93‑1 (MQ=255)
                          agcagTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACtt                                                >  2:4004059/1‑93 (MQ=255)
                           gcagTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTc                                               >  1:5030961/1‑93 (MQ=255)
                           gcagTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTc                                               <  1:6264596/93‑1 (MQ=255)
                            cagtcagtCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTca                                              <  1:4083179/93‑1 (MQ=255)
                             agtcagtcATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGGACTCCCTATAATGCGACCCCATCGACACGGCCGATGTGAATCCCTTcac                                             >  2:5413718/1‑93 (MQ=25)
                             agtcagtcATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTcac                                             <  1:1787125/93‑1 (MQ=255)
                              gtcagtcaTTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTcaca                                            <  1:4519718/93‑1 (MQ=255)
                               tcagtcaTTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTcacac                                           >  1:3188885/1‑93 (MQ=255)
                                cagtcaTTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTcacaca                                          >  2:520901/1‑93 (MQ=255)
                                cagtcaTTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTcacaca                                          >  1:3304507/1‑93 (MQ=255)
                                 agtcaTTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACaa                                         >  2:16595/1‑93 (MQ=255)
                                 agtcaTTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACaa                                         <  2:6015582/93‑1 (MQ=255)
                                  gtcaTTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACaaa                                        >  1:4189848/1‑93 (MQ=255)
                                   tcaTTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAAc                                       >  1:337315/1‑93 (MQ=255)
                                   tcaTTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAAc                                       <  1:2505462/93‑1 (MQ=255)
                                   tcaTTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAAc                                       <  2:1249534/93‑1 (MQ=255)
                                    caTTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACa                                      <  1:6232672/93‑1 (MQ=255)
                                    caTTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACa                                      <  2:2947582/93‑1 (MQ=255)
                                     aTTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAg                                     >  2:2346036/1‑93 (MQ=255)
                                     aTTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAg                                     >  1:1016623/1‑93 (MQ=255)
                                     aTTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAg                                     >  1:3925755/1‑93 (MQ=255)
                                      tttttCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGc                                    <  1:2419908/93‑1 (MQ=255)
                                      tttttCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGc                                    >  2:4910932/1‑93 (MQ=255)
                                       ttttCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGcc                                   >  1:3472870/1‑93 (MQ=255)
                                        tttCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCcg                                  >  1:265710/1‑93 (MQ=255)
                                          tCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCcggt                                >  2:5599369/1‑93 (MQ=255)
                                           cTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCcggtt                               <  2:403698/93‑1 (MQ=255)
                                           cTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCcggtt                               >  2:2606350/1‑93 (MQ=255)
                                           cTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCcggtt                               <  2:2893685/93‑1 (MQ=255)
                                           cTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCcggtt                               >  2:4304011/1‑93 (MQ=255)
                                            tgtaaTTTTTATTTTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCCCTTCACACAAACAGCcggttc                              <  2:2018193/90‑1 (MQ=17)
                                            tGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCcggttc                              <  2:2579569/93‑1 (MQ=255)
                                            tGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCcggttc                              <  1:1853561/93‑1 (MQ=255)
                                             gCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTACAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCcggttcg                             >  1:760911/1‑93 (MQ=255)
                                              cAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCcggttcgg                            <  1:2327804/93‑1 (MQ=255)
                                               aaTTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCcgggtcggt                           >  2:5444530/1‑93 (MQ=255)
                                                 tttttCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTg                         >  2:6252539/1‑93 (MQ=255)
                                                   tttCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGaa                       >  2:2071626/1‑93 (MQ=255)
                                                   tttCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGaa                       >  1:5919811/1‑93 (MQ=255)
                                                    ttCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAAAAGCCTGTTCGGTTgtaa                      >  2:591361/1‑90 (MQ=18)
                                                     tCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAaga                     >  2:1571449/1‑93 (MQ=255)
                                                     tCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAaga                     >  2:3699287/1‑93 (MQ=255)
                                                       tATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAgaga                   >  1:1057831/1‑93 (MQ=255)
                                                        aTTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGaa                  >  2:3736859/1‑93 (MQ=255)
                                                        aTTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGaa                  >  2:3287594/1‑93 (MQ=255)
                                                         ttGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGaaa                 >  2:4439761/1‑93 (MQ=255)
                                                         ttGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGGGAATCACTTCACACAAACAGCCGGCTCGGTTGAAGAGaaa                 >  1:1671177/1‑93 (MQ=255)
                                                          tGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGaaaa                >  2:4741123/1‑93 (MQ=255)
                                                             ggCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATc             >  1:1407808/1‑93 (MQ=255)
                                                             ggCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATc             <  2:2792985/93‑1 (MQ=255)
                                                             ggCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATc             >  1:4118514/1‑93 (MQ=255)
                                                              gCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATcc            >  1:4003682/1‑93 (MQ=255)
                                                              gCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATcc            >  2:5908401/1‑93 (MQ=255)
                                                              gCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATcc            >  1:1878618/1‑93 (MQ=255)
                                                               ccTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCt           <  2:4526458/93‑1 (MQ=255)
                                                                cTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAACCAGCCGGTTCGGTTGAAGAGAAAAATCCTg          >  1:1546141/1‑93 (MQ=255)
                                                                 tGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGa         <  2:3908697/93‑1 (MQ=255)
                                                                 tGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGa         >  1:3708750/1‑93 (MQ=255)
                                                                 tGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGa         <  2:1363559/93‑1 (MQ=255)
                                                                    ggAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAAt      <  2:4581302/93‑1 (MQ=25)
                                                                    ggAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAAt      >  2:4531476/1‑93 (MQ=25)
                                                                    ggAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAAt      <  1:4720345/93‑1 (MQ=25)
                                                                    ggAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAAt      >  2:5648446/1‑93 (MQ=25)
                                                                      agaACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTc    >  1:911295/1‑93 (MQ=18)
                                                                      agaACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTc    <  2:5432290/93‑1 (MQ=18)
                                                                      agaACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTc    <  1:3556691/93‑1 (MQ=18)
                                                                       gaACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCa   <  2:3607078/93‑1 (MQ=17)
                                                                       gaACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCa   >  2:1513531/1‑93 (MQ=17)
                                                                        aaCTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAg  <  1:2062707/93‑1 (MQ=14)
                                                                                          |                                                                          
TAAAGCGGGCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAG  >  NC_000913/4205348‑4205512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTATAAAGCGGGCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGA  >  NC_000913/4205345‑4205531
                                                                                             |                                                                                             
GTATAAAGCGGGCTTTTGCTGGGGAGTGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCC                                                                                               >  SRR4302533.5021456/1‑94 (MQ=60)
 TATAAAGCGGGCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCC                                                                                              >  SRR4302533.5876145/1‑94 (MQ=60)
 TATAAAGCGGGCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCC                                                                                              <  SRR4302533.3640989/94‑1 (MQ=60)
  ATAAAGCGGGCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCC                                                                                             <  SRR4302533.564472/94‑1 (MQ=60)
  ATAAAGCGGGCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCC                                                                                             <  SRR4302533.2145019/94‑1 (MQ=60)
  ATAAAGCGGGCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCC                                                                                             >  SRR4302533.5043037/1‑94 (MQ=60)
   TAAAGCGGGCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCA                                                                                            <  SRR4302533.3777831/94‑1 (MQ=60)
    AAAGCGGGCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCAT                                                                                           >  SRR4302533.4881586/1‑94 (MQ=60)
     AAGCGGGCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATC                                                                                          <  SRR4302533.5665574/94‑1 (MQ=60)
      AGCGGGCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCG                                                                                         >  SRR4302533.1822851/1‑94 (MQ=60)
       GCGGGCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGA                                                                                        >  SRR4302533.153195/1‑94 (MQ=60)
       GCGGGCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGA                                                                                        >  SRR4302533.3479321/1‑94 (MQ=60)
        CGGGCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGAC                                                                                       <  SRR4302533.4519370/94‑1 (MQ=60)
        CGGGCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGAC                                                                                       >  SRR4302533.1127842/1‑94 (MQ=60)
         GGGCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACA                                                                                      >  SRR4302533.92791/1‑94 (MQ=60)
          GGCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGCGAACTCCCTATAATGCGCCCCCATCGACAC                                                                                     >  SRR4302533.3441347/1‑94 (MQ=60)
           GCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACG                                                                                    <  SRR4302533.1658483/94‑1 (MQ=60)
           GCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACG                                                                                    <  SRR4302533.3584605/94‑1 (MQ=60)
           GCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACG                                                                                    <  SRR4302533.1816278/94‑1 (MQ=60)
            CTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGG                                                                                   <  SRR4302533.576895/94‑1 (MQ=60)
             TTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGC                                                                                  >  SRR4302533.5190799/1‑94 (MQ=60)
              TTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGACCTCCCTATAATGCGCCCCCATCGACACGGCG                                                                                 <  SRR4302533.5583215/94‑1 (MQ=60)
               TTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGG                                                                                >  SRR4302533.1527331/1‑94 (MQ=60)
                TGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGA                                                                               >  SRR4302533.3427105/1‑94 (MQ=60)
                  CTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGACCCCATCGACACGGCGGATG                                                                             >  SRR4302533.3318219/1‑94 (MQ=60)
                  CTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATAGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATG                                                                             >  SRR4302533.3405853/1‑94 (MQ=60)
                      GGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAA                                                                         <  SRR4302533.412299/94‑1 (MQ=60)
                          TGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCAC                                                                     <  SRR4302533.2903253/94‑1 (MQ=60)
                           GCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACT                                                                    >  SRR4302533.4806109/1‑94 (MQ=60)
                           GCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACT                                                                    <  SRR4302533.4367451/94‑1 (MQ=60)
                            CAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTT                                                                   <  SRR4302533.3850049/94‑1 (MQ=60)
                             AGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTC                                                                  >  SRR4302533.5061422/1‑94 (MQ=60)
                              GCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCA                                                                 <  SRR4302533.6302037/94‑1 (MQ=60)
                              GCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCA                                                                 >  SRR4302533.3622597/1‑94 (MQ=60)
                               CAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCAC                                                                <  SRR4302533.4108034/94‑1 (MQ=60)
                               CAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCAC                                                                >  SRR4302533.211959/1‑94 (MQ=60)
                               CAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCAC                                                                >  SRR4302533.183566/1‑94 (MQ=60)
                                AGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACA                                                               >  SRR4302533.2444905/1‑94 (MQ=60)
                                AGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACA                                                               <  SRR4302533.1797987/94‑1 (MQ=60)
                                 GTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACAC                                                              <  SRR4302533.4022407/94‑1 (MQ=60)
                                 GTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACAC                                                              >  SRR4302533.3208501/1‑94 (MQ=60)
                                 GTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACAC                                                              <  SRR4302533.4547087/94‑1 (MQ=60)
                                  TCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACA                                                             >  SRR4302533.3324913/1‑94 (MQ=60)
                                    AGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTGACCCAAA                                                           >  SRR4302533.1671855/1‑94 (MQ=60)
                                    AGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAA                                                           >  SRR4302533.4215211/1‑94 (MQ=60)
                                     GTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAAC                                                          >  SRR4302533.339375/1‑94 (MQ=60)
                                      TCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACA                                                         <  SRR4302533.2520907/94‑1 (MQ=60)
                                       CATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAG                                                        >  SRR4302533.3949725/1‑94 (MQ=60)
                                       CATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAG                                                        >  SRR4302533.1022599/1‑94 (MQ=60)
                                       CATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAG                                                        <  SRR4302533.6269995/94‑1 (MQ=60)
                                        ATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGC                                                       >  SRR4302533.3336730/1‑94 (MQ=60)
                                         TTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCC                                                      >  SRR4302533.3494300/1‑94 (MQ=60)
                                         TTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCC                                                      <  SRR4302533.2434854/94‑1 (MQ=60)
                                         TTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCC                                                      <  SRR4302533.939155/94‑1 (MQ=60)
                                          TTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCG                                                     >  SRR4302533.267327/1‑94 (MQ=60)
                                          TTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCG                                                     <  SRR4302533.144918/94‑1 (MQ=60)
                                            TTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGT                                                   <  SRR4302533.204228/94‑1 (MQ=60)
                                             TCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTT                                                  <  SRR4302533.6279648/94‑1 (MQ=60)
                                               TGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCG                                                <  SRR4302533.1864861/94‑1 (MQ=60)
                                               TGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTACAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCG                                                >  SRR4302533.765388/1‑94 (MQ=60)
                                                GCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGG                                               >  SRR4302533.4363738/1‑94 (MQ=60)
                                                 CAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGT                                              <  SRR4302533.81078/94‑1 (MQ=60)
                                                 CAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGT                                              <  SRR4302533.2342213/94‑1 (MQ=60)
                                                     TTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAA                                          >  SRR4302533.5955594/1‑94 (MQ=58)
                                                        TCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAG                                       <  SRR4302533.176212/94‑1 (MQ=50)
                                                         CTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGA                                      >  SRR4302533.1064053/1‑94 (MQ=48)
                                                           ATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGGGAATCACTTCACACAAACAGCCGGCTCGGTTGAAGAGAAA                                    >  SRR4302533.1681278/1‑94 (MQ=39)
                                                             TGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAA                                  <  SRR4302533.5244391/94‑1 (MQ=38)
                                                               CGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATC                                >  SRR4302533.4143529/1‑94 (MQ=33)
                                                               CGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATC                                <  SRR4302533.2365517/94‑1 (MQ=33)
                                                               CGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATC                                >  SRR4302533.1416320/1‑94 (MQ=33)
                                                               CGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATC                                >  SRR4302533.6267924/1‑94 (MQ=33)
                                                                GGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCC                               >  SRR4302533.4028058/1‑94 (MQ=30)
                                                                GGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCC                               >  SRR4302533.2870660/1‑94 (MQ=30)
                                                                GGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCC                               >  SRR4302533.1890067/1‑94 (MQ=30)
                                                                  CCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAACCAGCCGGTTCGGTTGAAGAGAAAAATCCTG                             >  SRR4302533.1555459/1‑94 (MQ=24)
                                                                  CCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTG                             <  SRR4302533.3853947/94‑1 (MQ=25)
                                                                   CTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGA                            >  SRR4302533.3731529/1‑94 (MQ=23)
                                                                     GCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAA                          <  SRR4302533.5086940/94‑1 (MQ=15)
                                                                       GGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATT                        <  SRR4302533.4748966/94‑1 (MQ=10)
                                                                       GGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATT                        <  SRR4302533.3512431/94‑1 (MQ=10)
                                                                        GAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTC                       >  SRR4302533.916638/1‑94 (MQ=7)
                                                                        GAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTC                       <  SRR4302533.927311/94‑1 (MQ=7)
                                                                         AGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCA                      <  SRR4302533.3578611/94‑1 (MQ=5)
                                                                         AGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCA                      >  SRR4302533.2972651/1‑94 (MQ=5)
                                                                           AACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGG                    <  SRR4302533.2075425/94‑1 (MQ=0)
                                                                            ACTCCCTATAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGG                   <  SRR4302533.4899390/94‑1 (MQ=0)
                                                                            ACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGG                   >  SRR4302533.314707/1‑94 (MQ=0)
                                                                             CTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGT                  >  SRR4302533.6196613/1‑94 (MQ=0)
                                                                              TCCCTATAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTT                 >  SRR4302533.3390376/1‑94 (MQ=0)
                                                                              TCCCTATAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTT                 <  SRR4302533.3371916/94‑1 (MQ=0)
                                                                              TCCCTATAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTT                 >  SRR4302533.3431317/1‑94 (MQ=0)
                                                                              TCCCTATAATGCGCCTCCATCGACACGGCGGATGTAAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTT                 >  SRR4302533.5089248/1‑94 (MQ=0)
                                                                              TCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTT                 >  SRR4302533.5179489/1‑94 (MQ=0)
                                                                               CCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGCTG                >  SRR4302533.359930/1‑94 (MQ=0)
                                                                                CCTATAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGA               <  SRR4302533.2094465/94‑1 (MQ=0)
                                                                                 CTATAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGAC              >  SRR4302533.4356261/1‑94 (MQ=0)
                                                                                  TATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACT             >  SRR4302533.2876216/1‑94 (MQ=0)
                                                                                    TAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCT           >  SRR4302533.4301159/1‑94 (MQ=0)
                                                                                    TAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCT           <  SRR4302533.2238721/94‑1 (MQ=0)
                                                                                    TAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCT           <  SRR4302533.6055003/94‑1 (MQ=0)
                                                                                    TAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCT           <  SRR4302533.295958/94‑1 (MQ=0)
                                                                                     AATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTG          >  SRR4302533.4830367/1‑94 (MQ=0)
                                                                                         CGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAG      <  SRR4302533.1971550/94‑1 (MQ=0)
                                                                                          GCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGA     >  SRR4302533.484911/1‑94 (MQ=0)
                                                                                          GCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGGTGACTCTGAAAGA     >  SRR4302533.1152117/1‑94 (MQ=0)
                                                                                           CCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAG    <  SRR4302533.3532141/94‑1 (MQ=0)
                                                                                           CCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAG    >  SRR4302533.5477953/1‑94 (MQ=0)
                                                                                           CCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAG    <  SRR4302533.5460960/94‑1 (MQ=0)
                                                                                           CCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAG    >  SRR4302533.6288097/1‑94 (MQ=0)
                                                                                            CTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGG   <  SRR4302533.4701774/94‑1 (MQ=0)
                                                                                            CTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGG   <  SRR4302533.4841616/94‑1 (MQ=0)
                                                                                            CTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGG   >  SRR4302533.5504227/1‑94 (MQ=0)
                                                                                            CTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGCTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGG   <  SRR4302533.4457610/94‑1 (MQ=0)
                                                                                            CCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGG   <  SRR4302533.4520739/94‑1 (MQ=0)
                                                                                             TCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGA  <  SRR4302533.86439/94‑1 (MQ=0)
                                                                                             TCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGA  >  SRR4302533.4792477/1‑94 (MQ=0)
                                                                                             CCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGA  <  SRR4302533.1147070/94‑1 (MQ=0)
                                                                                             |                                                                                             
GTATAAAGCGGGCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGA  >  NC_000913/4205345‑4205531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑