Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,307,603 T→C E319E (GAA→GAG)  yjcW ← putative ATP‑binding component of a transport system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,307,6030TC99.6% 735.6 / ‑5.9 231E319E (GAA→GAG) yjcWputative ATP‑binding component of a transport system
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (115/115);  minor base A (1/0);  total (116/115)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 245 total aligned reads displayed.

ATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTC  >  NC_000913/4307513‑4307692
                                                                                          |                                                                                         
   ccGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTcc                                                                                      >  1:1189589/1‑93 (MQ=255)
      gcggcTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGcc                                                                                   >  1:1480369/1‑93 (MQ=255)
       cgtcTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCa                                                                                  <  2:2472248/90‑1 (MQ=255)
       cggcTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTTCGCCa                                                                                  >  2:2400434/1‑93 (MQ=255)
         gcTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAgc                                                                                >  2:1157914/1‑93 (MQ=255)
            ttCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGcgcg                                                                             >  1:4875809/1‑93 (MQ=255)
             tCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGt                                                                            >  2:5289398/1‑93 (MQ=255)
             tCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGt                                                                            <  2:4114874/93‑1 (MQ=255)
              cAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGtt                                                                           >  1:3290175/1‑93 (MQ=255)
              cAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGtt                                                                           <  2:3330422/93‑1 (MQ=255)
                gTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTa                                                                         <  1:4506856/93‑1 (MQ=255)
                gTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTa                                                                         <  2:5618405/93‑1 (MQ=255)
                  gATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATc                                                                       <  2:4695580/93‑1 (MQ=255)
                  gATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATc                                                                       >  2:481678/1‑93 (MQ=255)
                  gATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATc                                                                       <  1:4089962/93‑1 (MQ=255)
                  gATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATc                                                                       <  1:3104667/93‑1 (MQ=255)
                        aGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGcc                                                                 >  1:3987489/1‑93 (MQ=255)
                         gCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCa                                                                >  2:6175639/1‑93 (MQ=255)
                           cATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCaaa                                                              >  1:2995542/1‑93 (MQ=255)
                            aTCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAAc                                                             >  1:981585/1‑93 (MQ=255)
                             tCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACa                                                            <  1:3444068/93‑1 (MQ=255)
                             tCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACa                                                            >  1:4157028/1‑93 (MQ=255)
                             tCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACa                                                            <  2:2618509/93‑1 (MQ=255)
                             tCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACa                                                            >  1:4735852/1‑93 (MQ=255)
                               ccTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGa                                                          >  1:3568016/1‑93 (MQ=255)
                               ccTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGa                                                          <  1:5538919/93‑1 (MQ=255)
                               ccTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGa                                                          <  1:5468458/93‑1 (MQ=255)
                               ccTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGa                                                          <  1:2105110/93‑1 (MQ=255)
                               ccTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGa                                                          <  1:6106251/93‑1 (MQ=255)
                                cTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGAc                                                         <  1:6106090/93‑1 (MQ=255)
                                 ttttttCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACa                                                        <  2:2111754/93‑1 (MQ=255)
                                  tttttCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACaa                                                       >  1:4661062/1‑93 (MQ=255)
                                    tttCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAAtt                                                     <  2:4955625/93‑1 (MQ=255)
                                    tttCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAAtt                                                     <  1:115291/93‑1 (MQ=255)
                                     ttCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATtc                                                    >  1:5213216/1‑93 (MQ=255)
                                     ttCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATtc                                                    >  1:2633268/1‑93 (MQ=255)
                                        aCGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATtcatc                                                 >  2:4441049/1‑93 (MQ=255)
                                         cGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATtcatca                                                <  2:4608214/93‑1 (MQ=255)
                                            cATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCAtcagtt                                             >  1:1366228/1‑93 (MQ=255)
                                              tCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCAtcagttca                                           >  2:3690915/1‑93 (MQ=255)
                                               ccAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCAtcagttcag                                          <  2:204959/93‑1 (MQ=255)
                                               ccAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCAtcagttcag                                          >  2:6093983/1‑93 (MQ=255)
                                                cAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCAtcagttcagt                                         <  2:651390/93‑1 (MQ=255)
                                                cAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCAtcagttcagt                                         >  1:4389363/1‑93 (MQ=255)
                                                cAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCAtcagttcaga                                         >  2:4260656/1‑92 (MQ=255)
                                                 aGGGGTGACCGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTa                                        >  2:6159792/1‑93 (MQ=255)
                                                 aGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTa                                        <  2:3409722/93‑1 (MQ=255)
                                                 aGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTa                                        >  2:4345501/1‑93 (MQ=255)
                                                 aGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTa                                        <  2:5553361/93‑1 (MQ=255)
                                                 aGGGGTGAACGTGGACAGATATCTTTGCCTGTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTa                                        <  2:3074840/93‑1 (MQ=255)
                                                  ggggTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCCGTTCAGTAc                                       >  2:3633440/1‑93 (MQ=255)
                                                  ggggTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTAc                                       >  2:2240334/1‑93 (MQ=255)
                                                  ggggTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTAc                                       <  2:5727639/93‑1 (MQ=255)
                                                  ggggTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTAc                                       >  2:2522483/1‑93 (MQ=255)
                                                  ggggTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTAc                                       >  2:5065317/1‑93 (MQ=255)
                                                      tGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTcc                                   >  2:521646/1‑93 (MQ=255)
                                                      tGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTcc                                   <  2:3940322/93‑1 (MQ=255)
                                                       gAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCg                                  >  1:4232297/1‑93 (MQ=255)
                                                        aaCGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCgg                                 <  1:5926778/93‑1 (MQ=255)
                                                           gTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAAc                              <  1:2914135/93‑1 (MQ=255)
                                                           gTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAAc                              <  2:5679877/93‑1 (MQ=255)
                                                           gTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAAc                              <  2:1494504/93‑1 (MQ=255)
                                                            tGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAAcc                             >  2:2867486/1‑93 (MQ=255)
                                                             ggAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCg                            >  2:2640494/1‑93 (MQ=255)
                                                             ggAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCg                            >  1:5121464/1‑93 (MQ=255)
                                                              gagagaTATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGa                           >  2:1332425/1‑93 (MQ=255)
                                                               agagaTATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGAc                          >  2:1053748/1‑93 (MQ=255)
                                                                gagaTATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGAcc                         <  1:117473/93‑1 (MQ=255)
                                                                 agaTATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCa                        >  1:799082/1‑93 (MQ=255)
                                                                 agaTATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCa                        <  1:924301/93‑1 (MQ=255)
                                                                  gaTATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAg                       >  1:2385202/1‑93 (MQ=255)
                                                                    tatCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTc                     >  2:4059748/1‑93 (MQ=255)
                                                                    tatCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTc                     >  1:1088425/1‑93 (MQ=255)
                                                                     atCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTcc                    <  2:3794362/93‑1 (MQ=255)
                                                                     atCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTcc                    <  1:3688145/93‑1 (MQ=255)
                                                                     atCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTcc                    >  1:4330418/1‑93 (MQ=255)
                                                                     atCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGACAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTcc                    >  2:2909877/1‑93 (MQ=255)
                                                                      tCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCg                   >  2:1946441/1‑93 (MQ=255)
                                                                      tCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCg                   >  1:2548743/1‑93 (MQ=255)
                                                                      tCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCg                   <  1:6191723/93‑1 (MQ=255)
                                                                       cTTTGCCAGTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCgg                  <  1:698507/93‑1 (MQ=255)
                                                                        tttGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGc                 >  1:5063778/1‑93 (MQ=255)
                                                                           gCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCaaa              >  1:1000050/1‑93 (MQ=255)
                                                                           gCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCaaa              >  1:1943288/1‑93 (MQ=255)
                                                                            ccATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAg             >  1:2245050/1‑93 (MQ=255)
                                                                            ccATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAg             >  1:5777353/1‑93 (MQ=255)
                                                                             cATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGc            <  2:4877737/93‑1 (MQ=255)
                                                                              aTTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGcc           >  1:4676748/1‑93 (MQ=255)
                                                                               ttAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCt          <  2:799893/93‑1 (MQ=255)
                                                                                tAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTa         <  2:2712232/93‑1 (MQ=255)
                                                                                tAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTa         <  2:3147813/93‑1 (MQ=255)
                                                                                 aaGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTaa        <  2:5910588/93‑1 (MQ=255)
                                                                                  aGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAat       >  2:5976773/1‑93 (MQ=255)
                                                                                   gACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAata      <  1:1893326/93‑1 (MQ=255)
                                                                                   aaCGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAata      <  1:216347/92‑1 (MQ=255)
                                                                                    aCGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAatat     <  2:1880165/93‑1 (MQ=255)
                                                                                    aCGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAatat     <  2:3552090/93‑1 (MQ=255)
                                                                                     cGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATAtt    <  2:1307735/93‑1 (MQ=255)
                                                                                       gATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTtc  >  2:5274812/1‑93 (MQ=255)
                                                                                       gATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTtc  <  2:4456261/93‑1 (MQ=255)
                                                                                          |                                                                                         
ATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTC  >  NC_000913/4307513‑4307692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCC  >  NC_000913/4307510‑4307696
                                                                                             |                                                                                             
GTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATC                                                                                               >  SRR4302533.1053867/1‑94 (MQ=60)
GTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATC                                                                                               <  SRR4302533.5598025/94‑1 (MQ=60)
GTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATC                                                                                               <  SRR4302533.5723201/94‑1 (MQ=60)
GTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATC                                                                                               <  SRR4302533.1363923/94‑1 (MQ=60)
GTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATC                                                                                               >  SRR4302533.1214792/1‑94 (MQ=60)
 TTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCT                                                                                              <  SRR4302533.749551/94‑1 (MQ=60)
  TATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTC                                                                                             <  SRR4302533.2781765/94‑1 (MQ=60)
     CCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCC                                                                                          >  SRR4302533.1196730/1‑94 (MQ=60)
      CCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCG                                                                                         <  SRR4302533.4405714/94‑1 (MQ=60)
       CGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGC                                                                                        >  SRR4302533.3351691/1‑94 (MQ=60)
        GGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCC                                                                                       >  SRR4302533.1489308/1‑94 (MQ=60)
         GCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCA                                                                                      <  SRR4302533.511485/94‑1 (MQ=60)
          CGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAG                                                                                     >  SRR4302533.3578169/1‑94 (MQ=60)
           GGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGC                                                                                    >  SRR4302533.4380371/1‑94 (MQ=60)
           GGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGC                                                                                    >  SRR4302533.6157281/1‑94 (MQ=60)
           GGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGC                                                                                    >  SRR4302533.1124472/1‑94 (MQ=60)
            GCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCG                                                                                   >  SRR4302533.2693634/1‑94 (MQ=60)
            GCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCG                                                                                   <  SRR4302533.51184/94‑1 (MQ=60)
              TTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCG                                                                                 >  SRR4302533.4905350/1‑94 (MQ=60)
              TTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCG                                                                                 >  SRR4302533.4905353/1‑94 (MQ=60)
                TCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTT                                                                               >  SRR4302533.3310489/1‑94 (MQ=60)
                 CAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTT                                                                              <  SRR4302533.2958681/94‑1 (MQ=60)
                  AGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTA                                                                             >  SRR4302533.206820/1‑94 (MQ=60)
                  AGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTA                                                                             >  SRR4302533.5638731/1‑94 (MQ=60)
                   GTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTAT                                                                            <  SRR4302533.4534152/94‑1 (MQ=60)
                   GTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTAT                                                                            <  SRR4302533.4733552/94‑1 (MQ=60)
                    TGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATC                                                                           <  SRR4302533.921919/94‑1 (MQ=60)
                     GATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCC                                                                          <  SRR4302533.4114860/94‑1 (MQ=60)
                     GATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCC                                                                          <  SRR4302533.3123753/94‑1 (MQ=60)
                     GATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCC                                                                          >  SRR4302533.4704479/1‑94 (MQ=60)
                      ATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCA                                                                         <  SRR4302533.4299917/94‑1 (MQ=60)
                       TGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCAC                                                                        <  SRR4302533.5310064/94‑1 (MQ=60)
                          AAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCC                                                                     >  SRR4302533.4011778/1‑94 (MQ=60)
                          AAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCC                                                                     <  SRR4302533.2469688/94‑1 (MQ=60)
                           AGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGAAGGATATCTTCGCCAGCGCGTTTATCCACGCCA                                                                    >  SRR4302533.1752667/1‑94 (MQ=60)
                             CCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAA                                                                  >  SRR4302533.3896671/1‑94 (MQ=60)
                             CCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAA                                                                  >  SRR4302533.3013861/1‑94 (MQ=60)
                              CATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGCTATCTTTGCCATTAAGACAGATCTCTCCGCCAGCGCGGCTATCCACGCCAAAC                                                                 >  SRR4302533.2631030/1‑94 (MQ=60)
                              CATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAAC                                                                 >  SRR4302533.987338/1‑94 (MQ=60)
                              CATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAAC                                                                 >  SRR4302533.2630154/1‑94 (MQ=60)
                               ATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACA                                                                >  SRR4302533.4182217/1‑94 (MQ=60)
                               ATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACA                                                                >  SRR4302533.4764560/1‑94 (MQ=60)
                                TCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAG                                                               >  SRR4302533.2992486/1‑94 (MQ=60)
                                TCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAG                                                               <  SRR4302533.3465339/94‑1 (MQ=60)
                                 CCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGA                                                              >  SRR4302533.3590004/1‑94 (MQ=60)
                                  CCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGAC                                                             <  SRR4302533.6143122/94‑1 (MQ=60)
                                  CCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGAC                                                             <  SRR4302533.5823419/94‑1 (MQ=60)
                                  CCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGAC                                                             <  SRR4302533.2118091/94‑1 (MQ=60)
                                  CCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGAC                                                             <  SRR4302533.5572365/94‑1 (MQ=60)
                                  CCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGAC                                                             <  SRR4302533.5501416/94‑1 (MQ=60)
                                   CTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACA                                                            <  SRR4302533.6142962/94‑1 (MQ=60)
                                   CTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACA                                                            <  SRR4302533.6142961/94‑1 (MQ=60)
                                    TTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAA                                                           >  SRR4302533.4689306/1‑94 (MQ=60)
                                     TTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAAT                                                          <  SRR4302533.5548965/94‑1 (MQ=60)
                                      TTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATT                                                         <  SRR4302533.4161681/94‑1 (MQ=60)
                                       TTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTC                                                        <  SRR4302533.116028/94‑1 (MQ=60)
                                       TTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTC                                                        >  SRR4302533.2649451/1‑94 (MQ=60)
                                       TTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTC                                                        >  SRR4302533.5244609/1‑94 (MQ=60)
                                       TTTCACGGCATCCAGGGGTAAACGTGCAGAGATATCTTTGCCATTAAGCCGGTTCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTC                                                        <  SRR4302533.4853761/94‑1 (MQ=60)
                                        TTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCA                                                       >  SRR4302533.6099099/1‑94 (MQ=60)
                                              GCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTT                                                 >  SRR4302533.1374441/1‑94 (MQ=60)
                                                ATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGATGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCA                                               >  SRR4302533.4604340/1‑94 (MQ=60)
                                                ATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCA                                               >  SRR4302533.5633794/1‑94 (MQ=60)
                                                ATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCA                                               <  SRR4302533.4188856/94‑1 (MQ=60)
                                                ATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCA                                               <  SRR4302533.3086310/94‑1 (MQ=60)
                                                 TCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAG                                              >  SRR4302533.2368785/1‑94 (MQ=60)
                                                  CCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGT                                             >  SRR4302533.4083891/1‑94 (MQ=60)
                                                  CCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGT                                             >  SRR4302533.2174953/1‑94 (MQ=60)
                                                  CCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGT                                             >  SRR4302533.4415863/1‑94 (MQ=60)
                                                  CCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGT                                             <  SRR4302533.1164861/94‑1 (MQ=60)
                                                    AGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTAC                                           <  SRR4302533.1171733/94‑1 (MQ=60)
                                                     GGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACG                                          <  SRR4302533.3273060/94‑1 (MQ=60)
                                                     GGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACG                                          <  SRR4302533.1791824/94‑1 (MQ=60)
                                                         TGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCG                                      >  SRR4302533.4257878/1‑94 (MQ=60)
                                                          GAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGG                                     >  SRR4302533.5236645/1‑94 (MQ=60)
                                                           AACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGA                                    <  SRR4302533.5962605/94‑1 (MQ=60)
                                                             CGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAAC                                  >  SRR4302533.951258/1‑94 (MQ=60)
                                                              GTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACC                                 <  SRR4302533.2932012/94‑1 (MQ=60)
                                                              GTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACC                                 >  SRR4302533.4065072/1‑94 (MQ=60)
                                                               TGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCG                                >  SRR4302533.5152407/1‑94 (MQ=60)
                                                                GGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGA                               >  SRR4302533.4843852/1‑94 (MQ=60)
                                                                  AGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACC                             <  SRR4302533.1320610/94‑1 (MQ=60)
                                                                  AGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACC                             >  SRR4302533.5342928/1‑94 (MQ=60)
                                                                   GAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCA                            >  SRR4302533.803785/1‑94 (MQ=60)
                                                                   GAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCA                            >  SRR4302533.801953/1‑94 (MQ=60)
                                                                   GAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCA                            >  SRR4302533.1365470/1‑94 (MQ=60)
                                                                   GAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCA                            <  SRR4302533.118220/94‑1 (MQ=60)
                                                                    AGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAG                           >  SRR4302533.220197/1‑94 (MQ=60)
                                                                    AGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAG                           <  SRR4302533.929729/94‑1 (MQ=60)
                                                                    AGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAG                           <  SRR4302533.6239169/94‑1 (MQ=60)
                                                                    AGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAG                           >  SRR4302533.2399942/1‑94 (MQ=60)
                                                                      ATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTC                         >  SRR4302533.1094836/1‑94 (MQ=60)
                                                                       TATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGATCAGTCC                        >  SRR4302533.687776/1‑94 (MQ=60)
                                                                       TATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCC                        >  SRR4302533.1376708/1‑94 (MQ=60)
                                                                       TATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCC                        >  SRR4302533.4356507/1‑94 (MQ=60)
                                                                        ATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCG                       <  SRR4302533.3710810/94‑1 (MQ=60)
                                                                        ATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCG                       >  SRR4302533.2564434/1‑94 (MQ=60)
                                                                        ATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCG                       <  SRR4302533.4235941/94‑1 (MQ=60)
                                                                         TCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGG                      <  SRR4302533.6228881/94‑1 (MQ=60)
                                                                          CTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGC                     >  SRR4302533.5094370/1‑94 (MQ=60)
                                                                          CTTTGCCAGTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGC                     <  SRR4302533.702639/94‑1 (MQ=60)
                                                                             TGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAA                  >  SRR4302533.1955141/1‑94 (MQ=60)
                                                                             TGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAA                  <  SRR4302533.1187508/94‑1 (MQ=60)
                                                                             TGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAA                  >  SRR4302533.1005887/1‑94 (MQ=60)
                                                                             TGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAA                  <  SRR4302533.3677519/94‑1 (MQ=60)
                                                                              GCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAG                 >  SRR4302533.2258923/1‑94 (MQ=60)
                                                                              GCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAG                 >  SRR4302533.5812248/1‑94 (MQ=60)
                                                                               CCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGC                <  SRR4302533.2090147/94‑1 (MQ=60)
                                                                                CATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCC               >  SRR4302533.4705092/1‑94 (MQ=60)
                                                                                CATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCC               >  SRR4302533.1923894/1‑94 (MQ=60)
                                                                                 ATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCT              <  SRR4302533.1052092/94‑1 (MQ=60)
                                                                                  TTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTA             <  SRR4302533.3501671/94‑1 (MQ=60)
                                                                                    AAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAAT           >  SRR4302533.2271135/1‑94 (MQ=60)
                                                                                    AAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAAT           <  SRR4302533.4611531/94‑1 (MQ=60)
                                                                                    AAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAAT           <  SRR4302533.895057/94‑1 (MQ=60)
                                                                                      GACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATAT         <  SRR4302533.4466301/94‑1 (MQ=60)
                                                                                      GACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATAT         <  SRR4302533.1904886/94‑1 (MQ=60)
                                                                                      AACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATAT         <  SRR4302533.217706/94‑1 (MQ=60)
                                                                                       ACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATT        <  SRR4302533.3125203/94‑1 (MQ=60)
                                                                                       ACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATT        >  SRR4302533.6001364/1‑94 (MQ=60)
                                                                                          GATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCT     <  SRR4302533.4817644/94‑1 (MQ=60)
                                                                                          GATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCT     >  SRR4302533.6274905/1‑94 (MQ=60)
                                                                                           ATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTC    <  SRR4302533.2441747/94‑1 (MQ=60)
                                                                                            TCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCC   <  SRR4302533.5468745/94‑1 (MQ=60)
                                                                                             CTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCACAGCCTAATATTTCTCCC  >  SRR4302533.1807080/1‑94 (MQ=60)
                                                                                             CTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCC  >  SRR4302533.843617/1‑94 (MQ=60)
                                                                                             CTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCC  >  SRR4302533.3219003/1‑94 (MQ=60)
                                                                                             CTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCC  >  SRR4302533.5890124/1‑94 (MQ=60)
                                                                                             |                                                                                             
GTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCC  >  NC_000913/4307510‑4307696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑