Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,356,994	C→T	A206T (GCA→ACA)	cadB ←	transport of lysine/cadaverine

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,356,994	0	C	T	100.0%	940.7 / NA	254	A206T (GCA→ACA)	cadB	transport of lysine/cadaverine
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (116/138); total (116/138)

Only 100 of 258 total aligned reads displayed.

TGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTATTCCAGTTA  >  NC_000913/4356904‑4357082

tGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTgg                                                                                        >  1:4820758/1‑93 (MQ=255)

tGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTgg                                                                                        >  1:3843070/1‑93 (MQ=255)

 gTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGa                                                                                       >  1:5912093/1‑93 (MQ=255)

 gTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGa                                                                                       <  1:5472400/93‑1 (MQ=255)

  tAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGAt                                                                                      <  2:123340/93‑1 (MQ=255)

   aaaCAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGAtt                                                                                     <  1:1259372/93‑1 (MQ=255)

    aaCAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTc                                                                                    <  1:1484982/93‑1 (MQ=255)

    aaCAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTc                                                                                    >  1:2012130/1‑93 (MQ=255)

       aaTACCTGTTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAac                                                                                 >  2:6199800/1‑93 (MQ=255)

       aaTACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAac                                                                                 <  1:1739770/93‑1 (MQ=255)

       aaTACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAac                                                                                 >  1:2255041/1‑93 (MQ=255)

          aCCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACAcc                                                                              >  1:249881/1‑93 (MQ=255)

          aCCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACAcc                                                                              <  2:2842957/93‑1 (MQ=255)

          aCCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACAcc                                                                              >  1:1037039/1‑93 (MQ=255)

          aCCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACAcc                                                                              <  1:4714448/93‑1 (MQ=255)

           ccTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACAccc                                                                             >  1:1563479/1‑93 (MQ=255)

           ccTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACAccc                                                                             >  1:2359644/1‑93 (MQ=255)

           acTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACCGTACGATTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAacactc                                                                             >  2:1885217/2‑91 (MQ=255)

            cTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCa                                                                            >  1:4658243/1‑93 (MQ=255)

            cTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCa                                                                            >  2:5573564/1‑93 (MQ=255)

                tAACCCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGaa                                                                        <  1:2657930/93‑1 (MQ=255)

                tAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGaa                                                                        >  2:4843964/1‑93 (MQ=255)

                tAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGaa                                                                        <  2:2333761/93‑1 (MQ=255)

                 aaaCCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAg                                                                       >  2:4492404/1‑93 (MQ=255)

                  aaCCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAgg                                                                      >  2:704194/1‑93 (MQ=255)

                  aaCCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAgg                                                                      <  1:4103082/93‑1 (MQ=255)

                    ccAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGcc                                                                    >  2:2389168/1‑93 (MQ=255)

                     cAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGccc                                                                   <  2:2938483/93‑1 (MQ=255)

                      aGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCca                                                                  <  1:5034621/93‑1 (MQ=255)

                       gTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCcac                                                                 <  2:348809/93‑1 (MQ=255)

                       gTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCcac                                                                 <  1:4058546/93‑1 (MQ=255)

                         aCCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAg                                                               <  1:3133567/93‑1 (MQ=255)

                          cccAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAgg                                                              <  1:4444381/93‑1 (MQ=255)

                           ccAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGgc                                                             >  2:2972959/1‑93 (MQ=255)

                           ccAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGgc                                                             <  1:273248/93‑1 (MQ=255)

                           caaGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGgc                                                             <  2:4928042/91‑1 (MQ=255)

                              gCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGgcaga                                                          <  1:4137711/93‑1 (MQ=255)

                              gCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGgcaga                                                          >  1:6090940/1‑93 (MQ=255)

                               cATGGCTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGgcagag                                                         <  1:1329434/93‑1 (MQ=255)

                                  ggTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGgcagagcag                                                      <  1:2148679/93‑1 (MQ=255)

                                  ggTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGgcagagcag                                                      >  1:4765271/1‑93 (MQ=255)

                                    ttGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGaa                                                    <  1:6182894/93‑1 (MQ=255)

                                       ccAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATAc                                                 <  2:5312065/93‑1 (MQ=255)

                                       ccAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATAc                                                 <  2:4647114/93‑1 (MQ=255)

                                         aGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACtt                                               <  2:3655265/93‑1 (MQ=255)

                                           cGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACtttt                                             <  1:5419158/93‑1 (MQ=255)

                                            ggAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTa                                            <  1:2849301/93‑1 (MQ=255)

                                            ggAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTa                                            >  2:2633557/1‑93 (MQ=255)

                                            ggAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTa                                            >  2:1479526/1‑93 (MQ=255)

                                             gAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTaa                                           >  2:215664/1‑93 (MQ=255)

                                             gAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTAAAGCTGTGGATTCAACACCCACGACGGCCCACAGGCAGAGCAGAATACTTTTaa                                           >  1:3051527/1‑93 (MQ=255)

                                               aCGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATg                                         <  1:4983962/93‑1 (MQ=255)

                                                cGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGa                                        <  2:5449027/93‑1 (MQ=255)

                                                cGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGa                                        <  1:1098770/93‑1 (MQ=255)

                                                cGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGa                                        <  2:4308585/93‑1 (MQ=255)

                                                 ggTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGAt                                       >  1:1049045/1‑93 (MQ=255)

                                                 ggTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGAt                                       <  1:6070685/93‑1 (MQ=255)

                                                 ggTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGAt                                       >  1:3888128/1‑93 (MQ=255)

                                                    aCGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCga                                    >  1:3448840/1‑92 (MQ=255)

                                                    aCGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGc                                    >  1:5620579/1‑93 (MQ=255)

                                                    aCGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGc                                    >  1:2898053/1‑93 (MQ=255)

                                                     cGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCa                                   <  1:3466994/93‑1 (MQ=255)

                                                      gTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCAt                                  >  2:4132723/1‑93 (MQ=255)

                                                      cttgcGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCAt                                  <  1:3508887/89‑1 (MQ=255)

                                                       tttCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGGTTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATg                                 >  1:3933904/1‑93 (MQ=255)

                                                         tCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGAc                               <  2:3071253/93‑1 (MQ=255)

                                                           gggTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCa                             >  1:3277902/1‑93 (MQ=255)

                                                            ggTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCAt                            >  1:3853895/1‑93 (MQ=255)

                                                            ggTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCAt                            >  1:3213780/1‑93 (MQ=255)

                                                            ggTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCAt                            <  1:4608081/93‑1 (MQ=255)

                                                             gTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATc                           >  1:6145036/1‑93 (MQ=255)

                                                               ttttAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAg                         <  2:3168815/93‑1 (MQ=255)

                                                                tttAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGt                        >  1:5803351/1‑93 (MQ=255)

                                                                  tAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTgg                      >  1:1602781/1‑93 (MQ=255)

                                                                  tAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTgg                      <  1:871350/93‑1 (MQ=255)

                                                                  tAACCATACCAGTACTTACAGCTGTGAATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTgg                      <  2:1594315/93‑1 (MQ=255)

                                                                   aaCCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGt                     <  2:4530440/93‑1 (MQ=255)

                                                                    aCCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTa                    <  1:1187544/93‑1 (MQ=255)

                                                                    aCCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTa                    >  2:588704/1‑93 (MQ=255)

                                                                     ccATACCAGTACTTACAGCTGTGGATTCCACACCCACGGAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGGGGTAt                   >  1:3639779/1‑93 (MQ=255)

                                                                     ccATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTAt                   <  2:5902414/93‑1 (MQ=255)

                                                                     ccATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTAt                   <  1:5276229/93‑1 (MQ=255)

                                                                      cATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATc                  <  1:4965804/93‑1 (MQ=255)

                                                                      cATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATc                  <  2:510934/93‑1 (MQ=255)

                                                                       aTACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATcc                 <  1:4386714/93‑1 (MQ=255)

                                                                        tACCAGTACTTACAGCTGTGGCTTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGGATTCg                >  2:397215/1‑93 (MQ=255)

                                                                        tACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCg                >  2:919890/1‑93 (MQ=255)

                                                                        tACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCg                >  2:5530170/1‑93 (MQ=255)

                                                                        aaCCAGAACTTGCAGCAGTGAATTCAACCCCCACGAAGACCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCg                <  2:5703522/92‑1 (MQ=255)

                                                                         aCCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGc               >  2:2068049/1‑93 (MQ=255)

                                                                          ccAGTACTTACAGCTGTGGCTTCAACACCCACGAACGCCCACAGGCACAGCAGAATACTTTTAATGATCGCATGACCATCAGAGGTATCCGCa              >  2:4979065/1‑93 (MQ=255)

                                                                          ccAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCa              <  1:812063/93‑1 (MQ=255)

                                                                             gTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAgga           >  2:1641718/1‑91 (MQ=255)

                                                                               aCTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTAtt         >  1:5163850/1‑93 (MQ=255)

                                                                               aCTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTAtt         <  2:3698161/93‑1 (MQ=255)

                                                                                cTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTATTc        >  1:671431/1‑93 (MQ=255)

                                                                                  tACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTATTCCa      <  1:3739748/93‑1 (MQ=255)

                                                                                   aCAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTATTCCAg     <  2:3364261/93‑1 (MQ=255)

                                                                                    cAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTATTCCAGt    >  2:3591396/1‑93 (MQ=255)

                                                                                      gCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTATTCCAGTTa  <  2:3220307/93‑1 (MQ=255)

TGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTATTCCAGTTA  >  NC_000913/4356904‑4357082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGC  >  NC_000913/4356901‑4357087

CGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGT                                                                                               >  SRR4302533.4021973/1‑94 (MQ=60)

CGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGT                                                                                               <  SRR4302533.1297514/94‑1 (MQ=60)

 GATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTG                                                                                              >  SRR4302533.2757729/1‑94 (MQ=60)

  ATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGG                                                                                             >  SRR4302533.3866546/1‑94 (MQ=60)

  ATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGG                                                                                             <  SRR4302533.2052479/94‑1 (MQ=60)

  ATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGG                                                                                             >  SRR4302533.4849970/1‑94 (MQ=60)

  ATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGGTTTTAACCATACCAGTACTTACAGCTGTGG                                                                                             >  SRR4302533.1089246/1‑94 (MQ=60)

   TGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGA                                                                                            >  SRR4302533.5947819/1‑94 (MQ=60)

    GTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGAT                                                                                           <  SRR4302533.1314383/94‑1 (MQ=60)

    GTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGAT                                                                                           <  SRR4302533.5137320/94‑1 (MQ=60)

    GTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGAT                                                                                           <  SRR4302533.5505380/94‑1 (MQ=60)

     TAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATT                                                                                          <  SRR4302533.5759045/94‑1 (MQ=60)

      AAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTC                                                                                         <  SRR4302533.1266903/94‑1 (MQ=60)

      AAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTC                                                                                         >  SRR4302533.2024525/1‑94 (MQ=60)

       AACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCA                                                                                        <  SRR4302533.1493944/94‑1 (MQ=60)

        ACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAA                                                                                       <  SRR4302533.5015913/94‑1 (MQ=60)

         CAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAAC                                                                                      >  SRR4302533.2886973/1‑94 (MQ=60)

         CAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAAC                                                                                      >  SRR4302533.2268988/1‑94 (MQ=60)

          AATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACA                                                                                     <  SRR4302533.1750319/94‑1 (MQ=60)

           ATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACAC                                                                                    <  SRR4302533.6214768/94‑1 (MQ=60)

            TACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACC                                                                                   >  SRR4302533.251422/1‑94 (MQ=60)

            TACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACC                                                                                   >  SRR4302533.1043135/1‑94 (MQ=60)

            AACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACC                                                                                   <  SRR4302533.5011550/94‑1 (MQ=60)

             ACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCC                                                                                  <  SRR4302533.4743020/94‑1 (MQ=60)

             ACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCC                                                                                  >  SRR4302533.1572890/1‑94 (MQ=60)

             ACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCC                                                                                  >  SRR4302533.2374227/1‑94 (MQ=60)

             ACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCC                                                                                  >  SRR4302533.5154479/1‑94 (MQ=60)

              CCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCA                                                                                 >  SRR4302533.292500/1‑94 (MQ=60)

              CCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCA                                                                                 >  SRR4302533.4686472/1‑94 (MQ=60)

              CCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCA                                                                                 <  SRR4302533.611021/94‑1 (MQ=60)

               CTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCAC                                                                                <  SRR4302533.6293482/94‑1 (MQ=60)

               CTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCAC                                                                                <  SRR4302533.2214223/94‑1 (MQ=60)

                TGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAAGCATACCAGTACTTACAGCTGTGGATTCAACACCCACG                                                                               <  SRR4302533.3650242/94‑1 (MQ=60)

                TGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACG                                                                               >  SRR4302533.1413934/1‑94 (MQ=60)

                TGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACG                                                                               >  SRR4302533.6020946/1‑94 (MQ=60)

                  CTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAA                                                                             >  SRR4302533.3159904/1‑94 (MQ=60)

                   TAACCCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAG                                                                            <  SRR4302533.2674259/94‑1 (MQ=60)

                   TAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAG                                                                            <  SRR4302533.4791581/94‑1 (MQ=60)

                     AACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGCAGGC                                                                          >  SRR4302533.2727563/1‑94 (MQ=60)

                     AACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGC                                                                          <  SRR4302533.4128044/94‑1 (MQ=60)

                      ACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCC                                                                         >  SRR4302533.1148941/1‑94 (MQ=60)

                         AGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCAC                                                                      <  SRR4302533.5065116/94‑1 (MQ=60)

                          GTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACA                                                                     <  SRR4302533.3390169/94‑1 (MQ=60)

                          GTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACA                                                                     <  SRR4302533.4083253/94‑1 (MQ=60)

                          GTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACA                                                                     >  SRR4302533.305563/1‑94 (MQ=60)

                           TACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAG                                                                    <  SRR4302533.6074030/94‑1 (MQ=60)

                           TACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAG                                                                    >  SRR4302533.4119859/1‑94 (MQ=60)

                           TACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAG                                                                    <  SRR4302533.3887616/94‑1 (MQ=60)

                            ACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGG                                                                   <  SRR4302533.3152832/94‑1 (MQ=60)

                             CCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGC                                                                  <  SRR4302533.2379266/94‑1 (MQ=60)

                             CCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGC                                                                  <  SRR4302533.4471263/94‑1 (MQ=60)

                              CCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCA                                                                 >  SRR4302533.3077997/1‑94 (MQ=60)

                              CCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCA                                                                 <  SRR4302533.274910/94‑1 (MQ=60)

                              CCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCA                                                                 <  SRR4302533.6145252/94‑1 (MQ=60)

                              CCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCA                                                                 >  SRR4302533.1698818/1‑94 (MQ=60)

                              CCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCA                                                                 >  SRR4302533.1530737/1‑94 (MQ=60)

                               CAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAG                                                                <  SRR4302533.4796633/94‑1 (MQ=60)

                               CAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAG                                                                >  SRR4302533.2311967/1‑94 (MQ=60)

                                AGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGA                                                               >  SRR4302533.6127754/1‑94 (MQ=60)

                                AGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGA                                                               <  SRR4302533.1261864/94‑1 (MQ=60)

                                AGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGA                                                               <  SRR4302533.1882466/94‑1 (MQ=60)

                                 GCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAG                                                              >  SRR4302533.4979067/1‑94 (MQ=60)

                                 GCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAG                                                              <  SRR4302533.3484098/94‑1 (MQ=60)

                                 GCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAG                                                              <  SRR4302533.3635155/94‑1 (MQ=60)

                                 GCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAG                                                              <  SRR4302533.4162815/94‑1 (MQ=60)

                                 GCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAG                                                              <  SRR4302533.5418725/94‑1 (MQ=60)

                                 GCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAG                                                              <  SRR4302533.5644359/94‑1 (MQ=60)

                                  CATGGCTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGC                                                             <  SRR4302533.1337411/94‑1 (MQ=60)

                                   ATGGTTTCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCA                                                            <  SRR4302533.1045082/94‑1 (MQ=60)

                                   ATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCA                                                            <  SRR4302533.780026/94‑1 (MQ=60)

                                   ATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCA                                                            >  SRR4302533.5430591/1‑94 (MQ=60)

                                    TGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAG                                                           >  SRR4302533.4794168/1‑94 (MQ=60)

                                     GGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGA                                                          >  SRR4302533.4600625/1‑94 (MQ=60)

                                     GGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGA                                                          <  SRR4302533.2161911/94‑1 (MQ=60)

                                       TTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAAT                                                        <  SRR4302533.6220019/94‑1 (MQ=60)

                                       TTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAAT                                                        <  SRR4302533.6168254/94‑1 (MQ=60)

                                       TTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAAT                                                        <  SRR4302533.3879004/94‑1 (MQ=60)

                                         GCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATAC                                                      >  SRR4302533.1712193/1‑94 (MQ=60)

                                           CAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTT                                                    <  SRR4302533.5030108/94‑1 (MQ=60)

                                           CAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTT                                                    <  SRR4302533.714866/94‑1 (MQ=60)

                                            AGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTT                                                   <  SRR4302533.3243583/94‑1 (MQ=60)

                                            AGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTT                                                   >  SRR4302533.4633770/1‑94 (MQ=60)

                                            AGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTCTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTT                                                   >  SRR4302533.4712302/1‑94 (MQ=60)

                                             GCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTT                                                  <  SRR4302533.3844709/94‑1 (MQ=60)

                                              CGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTA                                                 >  SRR4302533.5243718/1‑94 (MQ=60)

                                              CGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTA                                                 <  SRR4302533.5451759/94‑1 (MQ=60)

                                               GGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAA                                                <  SRR4302533.2866833/94‑1 (MQ=60)

                                               GGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAA                                                >  SRR4302533.5946974/1‑94 (MQ=60)

                                               GGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTAAAGCTGTGGATTCAACACCCACGACGGCCCACAGGCAGAGCAGAATACTTTTAA                                                >  SRR4302533.3070211/1‑94 (MQ=60)

                                               GGAACGGTACGTTTCGGGTTTTTAACCATACCAGGACTTACAGCTGTGGCTTCAACACCCACGAAGGCCCACAGGCAGAAGAGAAAACTTTTAA                                                >  SRR4302533.4313926/1‑94 (MQ=60)

                                                 AACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATG                                              <  SRR4302533.4675759/94‑1 (MQ=60)

                                                  ACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGA                                             <  SRR4302533.5014181/94‑1 (MQ=60)

                                                   CGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGAT                                            <  SRR4302533.3172966/94‑1 (MQ=60)

                                                   CGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGAT                                            <  SRR4302533.1105280/94‑1 (MQ=60)

                                                   CGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGAT                                            >  SRR4302533.1443135/1‑94 (MQ=60)

                                                   CGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGAT                                            <  SRR4302533.1499531/94‑1 (MQ=60)

                                                   CGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGAT                                            >  SRR4302533.3911852/1‑94 (MQ=60)

                                                   CGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGAT                                            >  SRR4302533.1055213/1‑94 (MQ=60)

                                                   CGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGAT                                            >  SRR4302533.3525003/1‑94 (MQ=60)

                                                   CGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGAT                                            <  SRR4302533.3796409/94‑1 (MQ=60)

                                                    GGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATC                                           >  SRR4302533.3936188/1‑94 (MQ=60)

                                                    GGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATC                                           <  SRR4302533.6107416/94‑1 (MQ=60)

                                                     GTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCG                                          <  SRR4302533.4042351/94‑1 (MQ=60)

                                                      TACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGC                                         >  SRR4302533.5654551/1‑94 (MQ=60)

                                                      TACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGC                                         >  SRR4302533.2915865/1‑94 (MQ=60)

                                                      TACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGA                                         >  SRR4302533.3470133/1‑94 (MQ=60)

                                                        CGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCAT                                       <  SRR4302533.3488392/94‑1 (MQ=60)

                                                        CGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCAT                                       <  SRR4302533.4012187/94‑1 (MQ=60)

                                                         cttgCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATG                                      <  SRR4302533.3530521/90‑1 (MQ=60)

                                                         GTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGGTTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATG                                      >  SRR4302533.3957915/1‑94 (MQ=60)

                                                          TTTCGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGA                                     <  SRR4302533.2452248/94‑1 (MQ=60)

                                                             CGGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCA                                  >  SRR4302533.3298144/1‑94 (MQ=60)

                                                              GGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCAT                                 >  SRR4302533.2871347/1‑94 (MQ=60)

                                                              GGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCAT                                 >  SRR4302533.3877435/1‑94 (MQ=60)

                                                              GGGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCAT                                 >  SRR4302533.3233589/1‑94 (MQ=60)

                                                               GGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATC                                >  SRR4302533.5467590/1‑94 (MQ=60)

                                                               GGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATC                                <  SRR4302533.4635989/94‑1 (MQ=60)

                                                               GGTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATC                                >  SRR4302533.6182037/1‑94 (MQ=60)

                                                                GTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCA                               >  SRR4302533.4451704/1‑94 (MQ=60)

                                                                GTTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCA                               >  SRR4302533.2584974/1‑94 (MQ=60)

                                                                 TTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAG                              <  SRR4302533.4148149/94‑1 (MQ=60)

                                                                 TTTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAG                              <  SRR4302533.387539/94‑1 (MQ=60)

                                                                  TTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGT                             >  SRR4302533.5838404/1‑94 (MQ=60)

                                                                  TTTTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGT                             <  SRR4302533.6330214/94‑1 (MQ=60)

                                                                    TTAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGG                           >  SRR4302533.1612487/1‑94 (MQ=60)

                                                                     TAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGT                          <  SRR4302533.1072825/94‑1 (MQ=60)

                                                                     TAACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGT                          <  SRR4302533.876465/94‑1 (MQ=60)

                                                                       ACCATACCAGTACTTACAGCTGTGGATTCCACACCCACGGAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGGGGTAT                        >  SRR4302533.3662159/1‑94 (MQ=60)

                                                                       ACCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTAT                        <  SRR4302533.1194670/94‑1 (MQ=60)

                                                                        CCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATC                       <  SRR4302533.5307862/94‑1 (MQ=60)

                                                                        CCATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATC                       >  SRR4302533.580346/1‑94 (MQ=60)

                                                                         CATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCC                      >  SRR4302533.2046771/1‑94 (MQ=60)

                                                                         CATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCC                      >  SRR4302533.4780438/1‑94 (MQ=60)

                                                                         CATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCC                      >  SRR4302533.1215325/1‑94 (MQ=60)

                                                                         CATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCC                      <  SRR4302533.4995906/94‑1 (MQ=60)

                                                                          ATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCG                     <  SRR4302533.4413200/94‑1 (MQ=60)

                                                                          ATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCG                     <  SRR4302533.232505/94‑1 (MQ=60)

                                                                          ATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCG                     >  SRR4302533.4126881/1‑94 (MQ=60)

                                                                          ATACCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCG                     >  SRR4302533.5528937/1‑94 (MQ=60)

                                                                             CCAGTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAG                  <  SRR4302533.816822/94‑1 (MQ=60)

                                                                                GTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTAT               <  SRR4302533.952407/94‑1 (MQ=60)

                                                                                GTACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTAT               <  SRR4302533.3667830/94‑1 (MQ=60)

                                                                                 TACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTATT              >  SRR4302533.5195030/1‑94 (MQ=60)

                                                                                 TACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTATT              <  SRR4302533.4962082/94‑1 (MQ=60)

                                                                                 TACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTATT              <  SRR4302533.2770089/94‑1 (MQ=60)

                                                                                  ACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTATTC             >  SRR4302533.675397/1‑94 (MQ=60)

                                                                                  ACTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTATTC             >  SRR4302533.4650829/1‑94 (MQ=60)

                                                                                   CTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTATTCC            >  SRR4302533.3527569/1‑94 (MQ=60)

                                                                                   CTTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTATTCC            <  SRR4302533.4189369/94‑1 (MQ=60)

                                                                                    TTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTATTCCA           >  SRR4302533.871312/1‑94 (MQ=60)

                                                                                    TTACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTATTCCA           <  SRR4302533.1574228/94‑1 (MQ=60)

                                                                                     TACAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTATTCCAG          <  SRR4302533.3762685/94‑1 (MQ=60)

                                                                                       CAGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCTCAGTATTCCAGTT        <  SRR4302533.3427827/94‑1 (MQ=60)

                                                                                        AGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTATTCCAGTTA       >  SRR4302533.2716792/1‑94 (MQ=60)

                                                                                        AGCTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTATTCCAGTTA       >  SRR4302533.2043745/1‑94 (MQ=60)

                                                                                          CTGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTATTCCAGTTAGC     >  SRR4302533.3478974/1‑94 (MQ=60)

                                                                                           TGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTATTCCAGTTAGCT    >  SRR4302533.5432508/1‑94 (MQ=60)

                                                                                           TGTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTATTCCAGTTAGCT    >  SRR4302533.4091605/1‑94 (MQ=60)

                                                                                            GTGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTATTCCAGTTAGCTG   <  SRR4302533.6024743/94‑1 (MQ=60)

                                                                                             TGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGC  >  SRR4302533.5926986/1‑94 (MQ=60)

CGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGC  >  NC_000913/4356901‑4357087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`