Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,363,427	T→C	T309A (ACT→GCT)	dcuA ←	anaerobic dicarboxylate transport

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,363,427	0	T	C	99.6%	820.1 / NA	252	T309A (ACT→GCT)	dcuA	anaerobic dicarboxylate transport
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (138/113); minor base G (1/0); total (139/113)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 259 total aligned reads displayed.

AGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGGTATCGA  >  NC_000913/4363337‑4363516

agaagaagaTGACGGCTAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCAt                                                                                         <  2:1646061/93‑1 (MQ=255)

 gaataagaCGCCGGCCAGCAGCGACGGATAAGACTGAATCCCTTCACCGGCGGTATCTTTGATCCCGTCGTTGTAGTTCGAAGAGAAAGCATc                                                                                        <  2:5226927/93‑1 (MQ=255)

 gaagaagaTGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATc                                                                                        >  2:1952640/1‑93 (MQ=255)

 gaagaagaTGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATc                                                                                        >  1:3508647/1‑93 (MQ=255)

 gaagaagaTGACGGCAAGCAGCCACGGACGACCCTGAATGACTTCACCAGCCGTATCTTTGATCCATGCGATGTTGTTGGAAACGAAAGTATc                                                                                        >  2:4089655/1‑93 (MQ=255)

  aagaagaTGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCg                                                                                       >  1:3285949/1‑93 (MQ=255)

   agaagaTGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGc                                                                                      <  2:4066615/93‑1 (MQ=255)

   agaagaTGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGCCGATGTTGTTGGAAACGAAAGCATCGc                                                                                      >  1:1490031/1‑93 (MQ=255)

    gaagaTGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGcc                                                                                     >  2:5750491/1‑93 (MQ=255)

     aagaTGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGccc                                                                                    <  1:5621825/93‑1 (MQ=255)

       gaTGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAg                                                                                  >  2:949685/1‑93 (MQ=255)

        aTGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGc                                                                                 >  1:1028771/1‑93 (MQ=255)

          gACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCa                                                                               >  2:3469869/1‑93 (MQ=255)

              gCCAGCAGCCACGGATGACCCTGAATCACTTCACCCGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCa                                                                           >  2:1863857/1‑93 (MQ=255)

              gCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCa                                                                           >  2:6022643/1‑93 (MQ=255)

              gCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCa                                                                           <  2:5318613/93‑1 (MQ=255)

                cagcagCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAac                                                                         <  2:1496392/93‑1 (MQ=255)

                   cagcCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACAcc                                                                      >  2:3375293/1‑93 (MQ=255)

                    agcAACGGATGACCCTGAATCACTACACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACAccc                                                                     <  2:4407553/93‑1 (MQ=255)

                     gcCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCa                                                                    >  1:4944457/1‑93 (MQ=255)

                     gcCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCa                                                                    >  1:3363861/1‑93 (MQ=255)

                     gcCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCa                                                                    <  2:2108589/93‑1 (MQ=255)

                        aCGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGa                                                                 >  1:3716827/1‑93 (MQ=255)

                         cGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGAt                                                                >  1:4894009/1‑93 (MQ=255)

                          ggATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATa                                                               >  2:3538025/1‑93 (MQ=255)

                          ggATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATa                                                               <  1:113332/93‑1 (MQ=255)

                           gATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCCCGCAACACCCAGGATAc                                                              >  1:5922608/1‑93 (MQ=255)

                           gATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATAc                                                              <  1:4702060/93‑1 (MQ=255)

                              gACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACaaa                                                           <  1:4231067/93‑1 (MQ=255)

                              gACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACaaa                                                           <  1:4814746/93‑1 (MQ=255)

                                cccTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATa                                                         >  1:3440787/1‑93 (MQ=255)

                                 ccTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATAc                                                        >  2:1775251/1‑93 (MQ=255)

                                 ccTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATAc                                                        <  1:587061/93‑1 (MQ=255)

                                 ccTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATAc                                                        <  1:5344637/93‑1 (MQ=255)

                                  cTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACa                                                       >  1:5872783/1‑93 (MQ=255)

                                  cTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACa                                                       <  1:2194733/93‑1 (MQ=255)

                                   tGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAg                                                      >  2:4144347/1‑93 (MQ=255)

                                    gAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAgg                                                     >  1:3264207/1‑93 (MQ=255)

                                    gAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAgg                                                     <  1:1262226/93‑1 (MQ=255)

                                    gAATCACTTCACCAGCGGTATCTGTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAgg                                                     <  1:2154923/93‑1 (MQ=255)

                                      aTCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGgcg                                                   >  2:2474807/1‑93 (MQ=255)

                                      aTCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGgcg                                                   >  2:2227670/1‑93 (MQ=255)

                                       tCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGgcgc                                                  >  1:3256888/1‑93 (MQ=255)

                                       tCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGgcgc                                                  >  1:594328/1‑93 (MQ=255)

                                       tCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGgcgc                                                  >  2:3752381/1‑93 (MQ=255)

                                        cACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCt                                                 <  1:2739411/93‑1 (MQ=255)

                                        cACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCt                                                 >  2:4844374/1‑93 (MQ=255)

                                         aCTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTc                                                >  2:4026187/1‑93 (MQ=255)

                                            tCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATa                                             >  2:1689812/1‑93 (MQ=255)

                                              aCCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATAcc                                           <  2:2380309/93‑1 (MQ=255)

                                                 aGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGc                                        >  1:3143163/1‑93 (MQ=255)

                                                 aGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGc                                        >  1:1762511/1‑93 (MQ=255)

                                                  gCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCt                                       >  1:719415/1‑93 (MQ=255)

                                                    ggTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCttt                                     >  1:2573978/1‑93 (MQ=255)

                                                    ggTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCttt                                     >  2:642835/1‑93 (MQ=255)

                                                    ggTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCttt                                     >  1:4413857/1‑93 (MQ=255)

                                                     gTATCTTTTATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATAACTGCTTTg                                    >  2:6141435/1‑93 (MQ=255)

                                                     gTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTg                                    >  2:2984959/1‑93 (MQ=255)

                                                     gTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTg                                    >  2:4507995/1‑93 (MQ=255)

                                                     gTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGAGACAAATACAGGCGCTCATACCTGCTTTg                                    >  1:1129835/1‑93 (MQ=255)

                                                      tATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGa                                   >  2:5866499/1‑93 (MQ=255)

                                                      tATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGa                                   <  1:2109854/93‑1 (MQ=255)

                                                       aTCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGaa                                  <  1:1388424/93‑1 (MQ=255)

                                                        tCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAg                                 >  2:2133656/1‑93 (MQ=255)

                                                        tCTGTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAg                                 <  2:5564433/93‑1 (MQ=255)

                                                         cTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATCCAGGCGCTCATACCTTCTTTGAAgg                                <  2:2825059/93‑1 (MQ=255)

                                                         cTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAgg                                <  2:1563713/93‑1 (MQ=255)

                                                          tttGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGt                               >  1:5050372/1‑93 (MQ=255)

                                                          tttGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGt                               <  1:1192345/93‑1 (MQ=255)

                                                          tttGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGt                               >  2:2694441/1‑93 (MQ=255)

                                                          tttGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGt                               >  2:1006311/1‑93 (MQ=255)

                                                             gATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCt                            >  1:663149/1‑93 (MQ=255)

                                                              aTCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTg                           >  1:696777/1‑93 (MQ=255)

                                                               tCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTgg                          >  2:2703833/1‑93 (MQ=255)

                                                                  aGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGt                       <  1:3764177/93‑1 (MQ=255)

                                                                  aGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGt                       <  1:2501643/93‑1 (MQ=255)

                                                                    tCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTg                     >  1:1003046/1‑93 (MQ=255)

                                                                        tgttgtTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGa                 <  2:3636994/93‑1 (MQ=255)

                                                                        tgttgtTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGa                 >  2:1965482/1‑93 (MQ=255)

                                                                        tgttgtTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGa                 >  2:668660/1‑93 (MQ=255)

                                                                        tgttgtTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGa                 >  1:4629337/1‑93 (MQ=255)

                                                                         gttgttGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGAt                <  1:1948767/93‑1 (MQ=255)

                                                                           tgttgGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGAtgt              >  2:2903812/1‑93 (MQ=255)

                                                                            gttgGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGAtgtt             >  2:4675571/1‑93 (MQ=255)

                                                                             ttgGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGAtgttg            >  2:4316026/1‑93 (MQ=255)

                                                                             ttgAAAACGAAAGCACCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGAtgttg            <  2:511058/93‑1 (MQ=255)

                                                                              tgGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGAtgttgt           >  2:5445538/1‑93 (MQ=255)

                                                                                 aaaCGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCgg        <  2:4916715/93‑1 (MQ=255)

                                                                                 aaaCGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCgg        <  2:1383475/93‑1 (MQ=255)

                                                                                  aaCGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGGt       <  1:3983793/93‑1 (MQ=255)

                                                                                   aCGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGGTa      <  2:3888395/93‑1 (MQ=255)

                                                                                   aCGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGGTa      >  1:4091939/1‑93 (MQ=255)

                                                                                   aCGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGGTa      <  1:1519772/93‑1 (MQ=255)

                                                                                   aCGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGGTa      <  1:5023092/93‑1 (MQ=255)

                                                                                     gAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGGTATc    <  2:5945905/93‑1 (MQ=255)

                                                                                      aaaGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGGTATCg   >  1:4330805/1‑93 (MQ=255)

                                                                                      aaaGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGGTATCg   >  1:5500009/1‑93 (MQ=255)

                                                                                      aaaGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGGTATCg   >  2:497209/1‑93 (MQ=255)

                                                                                       aaGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGGTATCGa  >  2:1155510/1‑93 (MQ=255)

                                                                                       aaGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGGTATCGa  >  1:888142/1‑93 (MQ=255)

AGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGGTATCGA  >  NC_000913/4363337‑4363516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGGTATCGACTTT  >  NC_000913/4363334‑4363520

gaaaaaaaaaagtgacggccaagaaccaaGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGC                                                                                               <  SRR4302533.257506/65‑1 (MQ=60)

CAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGC                                                                                               <  SRR4302533.1043084/94‑1 (MQ=60)

 AAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCA                                                                                              >  SRR4302533.3376972/1‑94 (MQ=60)

 AAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCA                                                                                              >  SRR4302533.4245647/1‑94 (MQ=60)

 AAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCA                                                                                              <  SRR4302533.3042988/94‑1 (MQ=60)

 AAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCA                                                                                              <  SRR4302533.5168791/94‑1 (MQ=60)

  AAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCAT                                                                                             >  SRR4302533.2145097/1‑94 (MQ=60)

  AAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCAT                                                                                             <  SRR4302533.2114363/94‑1 (MQ=60)

   gacccgctcttccgatctgctgtatCGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATC                                                                                            <  SRR4302533.5411902/69‑1 (MQ=60)

   AGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATC                                                                                            >  SRR4302533.4111249/1‑94 (MQ=60)

   AGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATC                                                                                            <  SRR4302533.5004284/94‑1 (MQ=60)

   AGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATC                                                                                            >  SRR4302533.3530279/1‑94 (MQ=60)

    GAAGAAGATGACGGCCAGCAGCCACGGATTACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAATCATCG                                                                                           <  SRR4302533.2222217/94‑1 (MQ=60)

    GAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCG                                                                                           >  SRR4302533.3306233/1‑94 (MQ=60)

     AAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGC                                                                                          <  SRR4302533.4976399/94‑1 (MQ=60)

     AAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGCCGATGTTGTTGGAAACGAAAGCATCGC                                                                                          >  SRR4302533.1499017/1‑94 (MQ=60)

        AAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCA                                                                                       <  SRR4302533.5655807/94‑1 (MQ=60)

         AGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTACCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAG                                                                                      <  SRR4302533.3397476/94‑1 (MQ=60)

          GATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGC                                                                                     >  SRR4302533.1034812/1‑94 (MQ=60)

           ATGACGGCCAGCAGCCACGGATGATCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCC                                                                                    >  SRR4302533.1078469/1‑94 (MQ=60)

           ATGACGCCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCC                                                                                    <  SRR4302533.4798221/94‑1 (MQ=60)

              ACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACG                                                                                 >  SRR4302533.5967510/1‑94 (MQ=60)

                GGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCA                                                                               >  SRR4302533.1490876/1‑94 (MQ=60)

                GGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCA                                                                               >  SRR4302533.3927401/1‑94 (MQ=60)

                 GCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAA                                                                              <  SRR4302533.1029289/94‑1 (MQ=60)

                 GCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAA                                                                              >  SRR4302533.5950832/1‑94 (MQ=60)

                 GCCAGCAGCCACGGATGACCCTGAATCACTTCAACAGCGGCATCTTTGATTCTGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAA                                                                              >  SRR4302533.2789897/1‑94 (MQ=60)

                  CCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAAC                                                                             >  SRR4302533.1942299/1‑94 (MQ=60)

                  CCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAAC                                                                             >  SRR4302533.3526501/1‑94 (MQ=60)

                   CAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACA                                                                            <  SRR4302533.2039733/94‑1 (MQ=60)

                   CAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACA                                                                            <  SRR4302533.710241/94‑1 (MQ=60)

                      CAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCC                                                                         <  SRR4302533.2730129/94‑1 (MQ=60)

                      CAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCC                                                                         <  SRR4302533.1136602/94‑1 (MQ=60)

                       AGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCA                                                                        >  SRR4302533.3384644/1‑94 (MQ=60)

                       AGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCA                                                                        >  SRR4302533.4974385/1‑94 (MQ=60)

                       AGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCA                                                                        <  SRR4302533.3277132/94‑1 (MQ=60)

                        GCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAG                                                                       <  SRR4302533.251340/94‑1 (MQ=60)

                         CCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATNGCCCAGCCACGCCACNCCCNGG                                                                      >  SRR4302533.5059065/1‑94 (MQ=60)

                         CCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGG                                                                      >  SRR4302533.1563573/1‑94 (MQ=60)

                         CCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGG                                                                      >  SRR4302533.3278911/1‑94 (MQ=60)

                          CACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGA                                                                     >  SRR4302533.3739642/1‑94 (MQ=60)

                          CACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGA                                                                     >  SRR4302533.797190/1‑94 (MQ=60)

                           ACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGAT                                                                    >  SRR4302533.400055/1‑94 (MQ=60)

                           ACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGAT                                                                    >  SRR4302533.4923670/1‑94 (MQ=60)

                           ACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGAT                                                                    <  SRR4302533.894488/94‑1 (MQ=60)

                            CGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATA                                                                   >  SRR4302533.1315348/1‑94 (MQ=60)

                             GGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCCCGCAACACCCAGGATAC                                                                  >  SRR4302533.5958409/1‑94 (MQ=60)

                             GGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATAC                                                                  >  SRR4302533.1721817/1‑94 (MQ=60)

                             GGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATAC                                                                  >  SRR4302533.427159/1‑94 (MQ=60)

                             GGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATAC                                                                  <  SRR4302533.114051/94‑1 (MQ=60)

                              GATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACA                                                                 >  SRR4302533.4668007/1‑94 (MQ=60)

                              GATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACA                                                                 <  SRR4302533.4730568/94‑1 (MQ=60)

                              GATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACA                                                                 >  SRR4302533.4967770/1‑94 (MQ=60)

                                 GACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAAT                                                              <  SRR4302533.4256642/94‑1 (MQ=60)

                                 GACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAAT                                                              <  SRR4302533.4843929/94‑1 (MQ=60)

                                  ACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATAgcaatc                                                             >  SRR4302533.685093/1‑88 (MQ=60)

                                  ACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATA                                                             <  SRR4302533.6016705/94‑1 (MQ=60)

                                  ACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATA                                                             >  SRR4302533.3462034/1‑94 (MQ=60)

                                  ACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATA                                                             <  SRR4302533.5116367/94‑1 (MQ=60)

                                    CCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACA                                                           >  SRR4302533.5312487/1‑94 (MQ=60)

                                    CCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACA                                                           <  SRR4302533.5376691/94‑1 (MQ=60)

                                    CCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACA                                                           <  SRR4302533.590528/94‑1 (MQ=60)

                                    CCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACA                                                           >  SRR4302533.5908279/1‑94 (MQ=60)

                                     CTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAG                                                          <  SRR4302533.2208257/94‑1 (MQ=60)

                                      TGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGG                                                         >  SRR4302533.3284341/1‑94 (MQ=60)

                                       GAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGC                                                        <  SRR4302533.1269775/94‑1 (MQ=60)

                                       GAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGC                                                        >  SRR4302533.4532305/1‑94 (MQ=60)

                                       GAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGC                                                        <  SRR4302533.1108091/94‑1 (MQ=60)

                                       GAATCACTTCACCAGCGGTATCTGTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGC                                                        <  SRR4302533.2168193/94‑1 (MQ=60)

                                        AATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCG                                                       >  SRR4302533.5617330/1‑94 (MQ=60)

                                        AATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCG                                                       >  SRR4302533.3578964/1‑94 (MQ=60)

                                         ATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGC                                                      >  SRR4302533.3276975/1‑94 (MQ=60)

                                         ATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGC                                                      <  SRR4302533.2522685/94‑1 (MQ=60)

                                         ATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGC                                                      >  SRR4302533.597833/1‑94 (MQ=60)

                                          TCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCT                                                     <  SRR4302533.3718709/94‑1 (MQ=60)

                                           CACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTC                                                    <  SRR4302533.2756208/94‑1 (MQ=60)

                                                CACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACC                                               <  SRR4302533.4406387/94‑1 (MQ=60)

                                                 ACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCT                                              <  SRR4302533.4604188/94‑1 (MQ=60)

                                                   CAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGC                                            >  SRR4302533.1773249/1‑94 (MQ=60)

                                                   CAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGC                                            >  SRR4302533.3162484/1‑94 (MQ=60)

                                                   CAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGC                                            >  SRR4302533.674012/1‑94 (MQ=60)

                                                    AGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCT                                           >  SRR4302533.723659/1‑94 (MQ=60)

                                                      CGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTT                                         >  SRR4302533.2398012/1‑94 (MQ=60)

                                                      CGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTT                                         <  SRR4302533.1247003/94‑1 (MQ=60)

                                                      CGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTT                                         <  SRR4302533.2173958/94‑1 (MQ=60)

                                                      CGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTT                                         >  SRR4302533.4440510/1‑94 (MQ=60)

                                                      CGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTT                                         >  SRR4302533.2589811/1‑94 (MQ=60)

                                                       GGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCCGGCTACAAATACCGGCGCTCATACCTGCTTTG                                        >  SRR4302533.5941243/1‑94 (MQ=60)

                                                       GGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTG                                        >  SRR4302533.1480963/1‑94 (MQ=60)

                                                       GGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGAGACAAATACAGGCGCTCATACCTGCTTTG                                        >  SRR4302533.1136550/1‑94 (MQ=60)

                                                         TATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAA                                      <  SRR4302533.2122869/94‑1 (MQ=60)

                                                          ATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAG                                     <  SRR4302533.5829802/94‑1 (MQ=60)

                                                          ATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAG                                     <  SRR4302533.1396783/94‑1 (MQ=60)

                                                          ATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAG                                     >  SRR4302533.3754578/1‑94 (MQ=60)

                                                            CTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGT                                   >  SRR4302533.5080916/1‑94 (MQ=60)

                                                             TTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTG                                  <  SRR4302533.1199511/94‑1 (MQ=60)

                                                             TTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTG                                  <  SRR4302533.4182328/94‑1 (MQ=60)

                                                               TGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCT                                >  SRR4302533.6138477/1‑94 (MQ=60)

                                                               TGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCT                                >  SRR4302533.667065/1‑94 (MQ=60)

                                                                GATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTG                               <  SRR4302533.320581/94‑1 (MQ=60)

                                                                GATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTG                               >  SRR4302533.700898/1‑94 (MQ=60)

                                                                GATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTG                               <  SRR4302533.4137018/94‑1 (MQ=60)

                                                                  TCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGA                             <  SRR4302533.1302458/94‑1 (MQ=60)

                                                                   CCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAG                            <  SRR4302533.1508849/94‑1 (MQ=60)

                                                                   CCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAG                            <  SRR4302533.2132151/94‑1 (MQ=60)

                                                                     AGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTT                          <  SRR4302533.3787248/94‑1 (MQ=60)

                                                                     AGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTT                          <  SRR4302533.2517072/94‑1 (MQ=60)

                                                                     AGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTT                          >  SRR4302533.669360/1‑94 (MQ=60)

                                                                      GTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTG                         >  SRR4302533.1142246/1‑94 (MQ=60)

                                                                      GTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTG                         >  SRR4302533.2780459/1‑94 (MQ=60)

                                                                      GTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTG                         >  SRR4302533.1008909/1‑94 (MQ=60)

                                                                      GTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTG                         <  SRR4302533.4892535/94‑1 (MQ=60)

                                                                      GTCGATGTTGTTGGAAACGAAAGCATAGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTG                         >  SRR4302533.3284414/1‑94 (MQ=60)

                                                                        CGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAG                       <  SRR4302533.3335257/94‑1 (MQ=60)

                                                                        CGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAG                       <  SRR4302533.2917139/94‑1 (MQ=60)

                                                                         GATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGG                      >  SRR4302533.4908377/1‑94 (MQ=60)

                                                                          ATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGA                     >  SRR4302533.4657395/1‑94 (MQ=60)

                                                                          ATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGA                     >  SRR4302533.350336/1‑94 (MQ=60)

                                                                            GTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATG                   >  SRR4302533.1178488/1‑94 (MQ=60)

                                                                            GTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATG                   <  SRR4302533.1960648/94‑1 (MQ=60)

                                                                               GTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTG                <  SRR4302533.874095/94‑1 (MQ=60)

                                                                               GTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTG                <  SRR4302533.4325841/94‑1 (MQ=60)

                                                                                  GGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCG             <  SRR4302533.5906649/94‑1 (MQ=60)

                                                                                   GAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGG            >  SRR4302533.2595171/1‑94 (MQ=60)

                                                                                     AACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGGTA          <  SRR4302533.4008050/94‑1 (MQ=60)

                                                                                     AACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGGTA          >  SRR4302533.4116847/1‑94 (MQ=60)

                                                                                      ACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGGTAT         <  SRR4302533.5053513/94‑1 (MQ=60)

                                                                                      ACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGGTAT         <  SRR4302533.1528922/94‑1 (MQ=60)

                                                                                      ACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGGTAT         <  SRR4302533.5488596/94‑1 (MQ=60)

                                                                                        GAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGGTATCG       <  SRR4302533.475601/94‑1 (MQ=60)

                                                                                        GAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGGTATCG       >  SRR4302533.2075955/1‑94 (MQ=60)

                                                                                        GAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGGTATCG       >  SRR4302533.4356897/1‑94 (MQ=60)

                                                                                        GAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGGTATCG       >  SRR4302533.3333074/1‑94 (MQ=60)

                                                                                        GAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGGTATCG       >  SRR4302533.5533187/1‑94 (MQ=60)

                                                                                         AAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGGTATCGA      >  SRR4302533.572836/1‑94 (MQ=60)

                                                                                         AAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGGTATCGA      >  SRR4302533.893356/1‑94 (MQ=60)

                                                                                          AAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGGTATCGAC     >  SRR4302533.3465892/1‑94 (MQ=60)

                                                                                           AGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGGTATCGACT    >  SRR4302533.5853674/1‑94 (MQ=60)

                                                                                             CATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGGTATCGACTTT  <  SRR4302533.2115743/94‑1 (MQ=60)

                                                                                             CATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGGTATCGACTTT  <  SRR4302533.3277811/94‑1 (MQ=60)

CAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGGTATCGACTTT  >  NC_000913/4363334‑4363520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`