Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,408,560	G→A	A144T (GCC→ACC)	yjfJ →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,408,560	0	G	A	99.6%	934.4 / NA	264	A144T (GCC→ACC)	yjfJ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (131/132); minor base T (0/1); total (131/133)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 270 total aligned reads displayed.

CTGGAAGTCTCCCGTGATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAGCCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGACAGCGGCAGAATCCTTA  >  NC_000913/4408470‑4408649

 tGGAAGTCTCCCGTGATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCa                                                                                        <  1:3925392/93‑1 (MQ=255)

 tGGAAGTCTCCCGTGATAGCGGGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCa                                                                                        >  2:2949006/1‑93 (MQ=255)

  ggAAGTCTCCCGTGATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCAc                                                                                       <  1:3008574/93‑1 (MQ=255)

   gAAGTCTCCCGTGATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACt                                                                                      <  1:73042/93‑1 (MQ=255)

   gAAGTCTCCCGTGATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACt                                                                                      >  2:586480/1‑93 (MQ=255)

   gAAGTCTCCCGTGATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACt                                                                                      <  2:5425822/93‑1 (MQ=255)

    aaGTCTCCCGTGATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTg                                                                                     <  1:3969297/93‑1 (MQ=255)

     aGTCTCCCGTGATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGa                                                                                    <  1:5079378/93‑1 (MQ=255)

      gTCTCCCGTGATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGaa                                                                                   <  2:1515441/93‑1 (MQ=255)

      gTCTCCCGTGATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGaa                                                                                   <  2:7458/93‑1 (MQ=255)

      gTCTCCCGTGATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGaa                                                                                   <  2:4352275/93‑1 (MQ=255)

         tcCCGTGATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGcc                                                                                >  1:1709582/1‑93 (MQ=255)

         tcCCGTGATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGcc                                                                                >  1:191233/1‑93 (MQ=255)

          cccGTGATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCa                                                                               <  1:1153211/93‑1 (MQ=255)

          cccGTGATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCa                                                                               <  2:3198193/93‑1 (MQ=255)

            cGTGATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATg                                                                             <  2:2741695/93‑1 (MQ=255)

             gTGATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGTCATGc                                                                            >  2:4095181/1‑93 (MQ=255)

             gTGATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGc                                                                            >  2:2398755/1‑93 (MQ=255)

             gTGATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGc                                                                            >  2:2236217/1‑93 (MQ=255)

             gTGATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGc                                                                            >  2:5952797/1‑93 (MQ=255)

               gATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAg                                                                          <  1:4800896/93‑1 (MQ=255)

                aTGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGc                                                                         >  1:127818/1‑93 (MQ=255)

                 tGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCg                                                                        <  1:5460848/93‑1 (MQ=255)

                   gCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTg                                                                      >  2:5683467/1‑93 (MQ=255)

                    cGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGc                                                                     >  2:3839089/1‑93 (MQ=255)

                       ggAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGcaca                                                                  <  1:5213490/93‑1 (MQ=255)

                        gAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCacaa                                                                 <  1:4723345/93‑1 (MQ=255)

                           aaaGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAg                                                              <  2:2519525/93‑1 (MQ=255)

                            aaGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAgg                                                             >  2:3548433/1‑93 (MQ=255)

                            aaGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAgg                                                             >  1:780957/1‑93 (MQ=255)

                             aGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGc                                                            >  2:3682743/1‑93 (MQ=255)

                              gCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCt                                                           >  1:3629410/1‑93 (MQ=255)

                                aGTGACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGt                                                         <  1:1384161/93‑1 (MQ=255)

                                 gTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTa                                                        >  1:2226039/1‑93 (MQ=255)

                                 gTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTa                                                        <  2:2378336/93‑1 (MQ=255)

                                 gTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTa                                                        <  1:3178037/93‑1 (MQ=255)

                                 gTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTa                                                        >  1:2971610/1‑93 (MQ=255)

                                  ttACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTaa                                                       <  1:944878/93‑1 (MQ=255)

                                  ttACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTaa                                                       >  1:2708965/1‑93 (MQ=255)

                                      agcgacagcgCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTaacaac                                                   <  1:3299540/93‑1 (MQ=255)

                                         gacagcgCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACctc                                                <  2:2115843/93‑1 (MQ=255)

                                         gacagcgCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACctc                                                >  2:1988961/1‑93 (MQ=255)

                                         gacagcgCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACctc                                                <  1:2339092/93‑1 (MQ=255)

                                          acagcgCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAGCctct                                               <  2:3285832/93‑1 (MQ=255)

                                          acagcgCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACctct                                               >  2:3352566/1‑93 (MQ=255)

                                          acagcgCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACctct                                               >  2:361613/1‑93 (MQ=255)

                                          acagcgCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACctct                                               >  1:325657/1‑93 (MQ=255)

                                          acagcgCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGGCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACctct                                               >  2:5134919/1‑93 (MQ=255)

                                           cagcgcagcgTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTa                                              <  2:607160/93‑1 (MQ=255)

                                            agcgcagcgTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTAc                                             <  2:2580632/93‑1 (MQ=255)

                                            agcgcagcgTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTAc                                             >  1:814490/1‑93 (MQ=255)

                                            agcgcagcgTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTAc                                             <  2:1127748/93‑1 (MQ=255)

                                                 agcgTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTg                                        >  1:1760620/1‑93 (MQ=255)

                                                 agcgTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTg                                        <  1:2040213/93‑1 (MQ=255)

                                                 agcgTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTg                                        <  2:5720564/93‑1 (MQ=255)

                                                  gcgTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAGACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTgg                                       <  2:824168/93‑1 (MQ=255)

                                                  gcgTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTgg                                       >  1:5493845/1‑93 (MQ=255)

                                                    gTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGgcg                                     >  1:3869643/1‑93 (MQ=255)

                                                    gTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGgcg                                     <  1:4944235/93‑1 (MQ=255)

                                                     tATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGgcgc                                    >  2:4131727/1‑93 (MQ=255)

                                                     tATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGgcgc                                    >  2:1101200/1‑93 (MQ=255)

                                                      aTCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCa                                   <  2:2315001/93‑1 (MQ=255)

                                                      aTCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCa                                   >  2:336837/1‑93 (MQ=255)

                                                      aTCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCa                                   <  2:2780688/93‑1 (MQ=255)

                                                       tCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCAt                                  <  2:4295173/93‑1 (MQ=255)

                                                        ctcTCAGTTTGAGCAGCAAATGGAAGTCGTAAAAACCACTGAGGCCGTGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATc                                 <  2:5399499/91‑1 (MQ=255)

                                                        cGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATc                                 >  1:1427920/1‑93 (MQ=255)

                                                           tCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTc                              >  1:5541330/1‑93 (MQ=255)

                                                           tCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTc                              <  1:3402064/93‑1 (MQ=255)

                                                            cAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTcc                             <  1:2464161/93‑1 (MQ=255)

                                                            cAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTcc                             <  1:4673033/93‑1 (MQ=255)

                                                            cAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTcc                             >  1:4393889/1‑93 (MQ=255)

                                                              gTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAg                           >  1:5595642/1‑93 (MQ=255)

                                                              gTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAg                           >  2:2989538/1‑93 (MQ=255)

                                                              gTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAg                           >  1:1997388/1‑93 (MQ=255)

                                                               tttGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGc                          >  1:4167135/1‑93 (MQ=255)

                                                                 tGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGt                        >  1:1568872/1‑93 (MQ=255)

                                                                 tGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGt                        <  1:3896627/93‑1 (MQ=255)

                                                                 tGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGt                        <  2:2078229/93‑1 (MQ=255)

                                                                  gAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGtt                       >  1:1094439/1‑93 (MQ=255)

                                                                  gAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGtt                       <  1:5813752/93‑1 (MQ=255)

                                                                    gcagcaAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTc                     >  1:6060827/1‑93 (MQ=255)

                                                                     cagcaAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCg                    >  1:1847488/1‑93 (MQ=255)

                                                                      agcaAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGa                   >  1:5869265/1‑93 (MQ=255)

                                                                       gcaAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGAc                  >  2:1416875/1‑93 (MQ=255)

                                                                        caAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGACa                 <  2:6212815/93‑1 (MQ=255)

                                                                         aaaTGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGACAg                >  2:1871711/1‑93 (MQ=255)

                                                                         aaaTGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGACAg                <  1:5035734/93‑1 (MQ=255)

                                                                          aaTGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGACAGc               >  2:4586289/1‑93 (MQ=255)

                                                                          aaTGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGACAGc               <  2:54763/93‑1 (MQ=255)

                                                                          aaTGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGACAGc               >  2:3732456/1‑93 (MQ=255)

                                                                           aTGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGACAGCg              >  1:3079773/1‑93 (MQ=255)

                                                                           aTGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGACAGCg              <  2:4900839/93‑1 (MQ=255)

                                                                            tGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTATCAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGACAGCgg             <  1:4444876/93‑1 (MQ=255)

                                                                            tGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGACAGCgg             <  2:634693/93‑1 (MQ=255)

                                                                                 gTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGACAGCGGCAGaa        <  2:557688/93‑1 (MQ=255)

                                                                                  tCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGACAGCGGCAGAAt       <  2:5659616/93‑1 (MQ=255)

                                                                                  tCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGACAGCGGCAGAAt       >  2:4715721/1‑93 (MQ=255)

                                                                                   cGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGACAGCGGCAGAATc      <  2:5376518/93‑1 (MQ=255)

                                                                                     ttAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGACAGCGGCAGAATCCt    >  1:3579441/1‑93 (MQ=255)

CTGGAAGTCTCCCGTGATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAGCCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGACAGCGGCAGAATCCTTA  >  NC_000913/4408470‑4408649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AATCTGGAAGTCTCCCGTGATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAGCCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGACAGCGGCAGAATCCTTAAAAC  >  NC_000913/4408467‑4408653

AATCTGGAAGTCTCCCGTGATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAA                                                                                               >  SRR4302533.4612495/1‑94 (MQ=60)

 ATCTGGAAGTCTCCCGTGATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAAC                                                                                              >  SRR4302533.4751208/1‑94 (MQ=60)

 ATCTGGAAGTCTCCCGTGATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAAC                                                                                              <  SRR4302533.3887777/94‑1 (MQ=60)

  TCTGGAAGTCTCCCGTGATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACC                                                                                             <  SRR4302533.687911/94‑1 (MQ=60)

   CTGGAAGTCTCCCGTGATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCA                                                                                            >  SRR4302533.1119338/1‑94 (MQ=60)

    TGGAAGTCTCCCGTGATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCAC                                                                                           <  SRR4302533.1901901/94‑1 (MQ=60)

    TGGAAGTCTCCCGTGATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCAC                                                                                           <  SRR4302533.3949362/94‑1 (MQ=60)

    TGGAAGTCTCCCGTGATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCAC                                                                                           <  SRR4302533.3908309/94‑1 (MQ=60)

     GGAAGTCTCCCGTGATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACT                                                                                          <  SRR4302533.3026979/94‑1 (MQ=60)

      GAAGTCTCCCGTGATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTG                                                                                         <  SRR4302533.73511/94‑1 (MQ=60)

      GAAGTCTCCCGTGATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTG                                                                                         >  SRR4302533.2251827/1‑94 (MQ=60)

       AAGTCTCCCGTGATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGA                                                                                        <  SRR4302533.3993474/94‑1 (MQ=60)

       AAGTCTCCCGTGATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGA                                                                                        >  SRR4302533.130066/1‑94 (MQ=60)

        AGTCTCCCGTGATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAA                                                                                       <  SRR4302533.5110077/94‑1 (MQ=60)

           CTCCCGTGATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCC                                                                                    >  SRR4302533.1719942/1‑94 (MQ=60)

           CTCCCGTGATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCC                                                                                    <  SRR4302533.317358/94‑1 (MQ=60)

           CTCCCGTGATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCC                                                                                    >  SRR4302533.4862173/1‑94 (MQ=60)

           CTCCCGTGATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCC                                                                                    >  SRR4302533.192434/1‑94 (MQ=60)

            TCCCGTGATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGCTAAAACCACTGAAGCCA                                                                                   >  SRR4302533.3219466/1‑94 (MQ=60)

             CCCGTGATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCAT                                                                                  <  SRR4302533.1160118/94‑1 (MQ=60)

              CCGTGATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATG                                                                                 <  SRR4302533.5946016/94‑1 (MQ=60)

               CGTGATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGC                                                                                >  SRR4302533.2127326/1‑94 (MQ=60)

                 TGATGGTGTTGAAAAAACTGTTACAGCGACGGCGCAGCGTATCGTTCAGTCTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAG                                                                              <  SRR4302533.3395930/94‑1 (MQ=60)

                 TGATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAG                                                                              >  SRR4302533.4003861/1‑94 (MQ=60)

                 TGATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAG                                                                              <  SRR4302533.4677800/94‑1 (MQ=60)

                  GATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGC                                                                             <  SRR4302533.4829976/94‑1 (MQ=60)

                  GATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGC                                                                             >  SRR4302533.128616/1‑94 (MQ=60)

                   ATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCG                                                                            <  SRR4302533.3089702/94‑1 (MQ=60)

                   ATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCG                                                                            <  SRR4302533.3413489/94‑1 (MQ=60)

                   ATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCG                                                                            <  SRR4302533.4055220/94‑1 (MQ=60)

                    TGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGT                                                                           <  SRR4302533.5493748/94‑1 (MQ=60)

                    TGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGT                                                                           <  SRR4302533.1755634/94‑1 (MQ=60)

                     GGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTG                                                                          <  SRR4302533.1533872/94‑1 (MQ=60)

                     GGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTG                                                                          >  SRR4302533.203310/1‑94 (MQ=60)

                       CGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCA                                                                        <  SRR4302533.3881006/94‑1 (MQ=60)

                        GTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCAC                                                                       >  SRR4302533.835201/1‑94 (MQ=60)

                         TGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACA                                                                      >  SRR4302533.85208/1‑94 (MQ=60)

                         TGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACA                                                                      <  SRR4302533.3318615/94‑1 (MQ=60)

                          GGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAA                                                                     <  SRR4302533.5244886/94‑1 (MQ=60)

                           GAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGCGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAAC                                                                    <  SRR4302533.3633603/94‑1 (MQ=60)

                           GAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAATCCACTGAAGCCATGCAGCGTGCACAAC                                                                    <  SRR4302533.5263832/94‑1 (MQ=60)

                           GAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAAC                                                                    <  SRR4302533.4751982/94‑1 (MQ=60)

                             AAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAG                                                                  <  SRR4302533.5518621/94‑1 (MQ=60)

                             AAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAG                                                                  >  SRR4302533.5892775/1‑94 (MQ=60)

                              AAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGG                                                                 >  SRR4302533.785557/1‑94 (MQ=60)

                               AAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGC                                                                >  SRR4302533.4360896/1‑94 (MQ=60)

                                AGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCT                                                               >  SRR4302533.3651742/1‑94 (MQ=60)

                                  CAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTAGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGT                                                             >  SRR4302533.4468121/1‑94 (MQ=60)

                                   AGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTA                                                            >  SRR4302533.2239780/1‑94 (MQ=60)

                                   AGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTA                                                            >  SRR4302533.2989791/1‑94 (MQ=60)

                                   AGTGACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTA                                                            <  SRR4302533.1392489/94‑1 (MQ=60)

                                    GTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAA                                                           >  SRR4302533.2725587/1‑94 (MQ=60)

                                    GTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAA                                                           <  SRR4302533.3197585/94‑1 (MQ=60)

                                     TTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAAC                                                          <  SRR4302533.950435/94‑1 (MQ=60)

                                     TTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAAC                                                          <  SRR4302533.3979945/94‑1 (MQ=60)

                                     TTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAAC                                                          <  SRR4302533.1250750/94‑1 (MQ=60)

                                         AGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACC                                                      <  SRR4302533.3319902/94‑1 (MQ=60)

                                            GACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCT                                                   <  SRR4302533.2353576/94‑1 (MQ=60)

                                            GACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCT                                                   >  SRR4302533.327643/1‑94 (MQ=60)

                                             ACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTA                                                  <  SRR4302533.4852482/94‑1 (MQ=60)

                                              CAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTAC                                                 >  SRR4302533.819257/1‑94 (MQ=60)

                                               AGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACGACAGGCTGTAACAACCTCTACC                                                >  SRR4302533.4410885/1‑94 (MQ=60)

                                                GCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCG                                               <  SRR4302533.5781876/94‑1 (MQ=60)

                                                 CGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGT                                              >  SRR4302533.1667900/1‑94 (MQ=60)

                                                 CGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGT                                              >  SRR4302533.4573751/1‑94 (MQ=60)

                                                 CGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGT                                              >  SRR4302533.5951110/1‑94 (MQ=60)

                                                  GCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTT                                             >  SRR4302533.3376665/1‑94 (MQ=60)

                                                  GCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTT                                             >  SRR4302533.5239207/1‑94 (MQ=60)

                                                  CCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTT                                             <  SRR4302533.142651/94‑1 (MQ=60)

                                                   CAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTG                                            >  SRR4302533.1771351/1‑94 (MQ=60)

                                                   CAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTG                                            <  SRR4302533.320870/94‑1 (MQ=60)

                                                   CAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTG                                            <  SRR4302533.2268136/94‑1 (MQ=60)

                                                    AGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGG                                           >  SRR4302533.5526984/1‑94 (MQ=60)

                                                    AGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGG                                           <  SRR4302533.2527357/94‑1 (MQ=60)

                                                    AGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGG                                           <  SRR4302533.5620010/94‑1 (MQ=60)

                                                    AGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGG                                           <  SRR4302533.2052791/94‑1 (MQ=60)

                                                    AGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGG                                           >  SRR4302533.2969991/1‑94 (MQ=60)

                                                    AGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCAGGCAGCGTGCACAACGGGCTGTAACAACCTCTACCGTTGG                                           <  SRR4302533.1875190/94‑1 (MQ=60)

                                                     GCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGC                                          <  SRR4302533.3903384/94‑1 (MQ=60)

                                                     GCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGC                                          <  SRR4302533.5533989/94‑1 (MQ=60)

                                                      CGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCG                                         >  SRR4302533.3893267/1‑94 (MQ=60)

                                                       GTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGC                                        <  SRR4302533.4974163/94‑1 (MQ=60)

                                                         ATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCAT                                      <  SRR4302533.4717209/94‑1 (MQ=60)

                                                         ATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCAT                                      >  SRR4302533.2536919/1‑94 (MQ=60)

                                                          TCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATC                                     <  SRR4302533.630364/94‑1 (MQ=60)

                                                          TCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATC                                     >  SRR4302533.1436551/1‑94 (MQ=60)

                                                           CGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCT                                    >  SRR4302533.3555349/1‑94 (MQ=60)

                                                            GCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTT                                   >  SRR4302533.3967481/1‑94 (MQ=60)

                                                             CTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTC                                  >  SRR4302533.2427593/1‑94 (MQ=60)

                                                             CTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTC                                  >  SRR4302533.5574793/1‑94 (MQ=60)

                                                             CTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTC                                  >  SRR4302533.800556/1‑94 (MQ=60)

                                                              TCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCC                                 >  SRR4302533.4420415/1‑94 (MQ=60)

                                                              TCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCC                                 <  SRR4302533.2094011/94‑1 (MQ=60)

                                                              TCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCC                                 <  SRR4302533.3423070/94‑1 (MQ=60)

                                                              TCAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCC                                 >  SRR4302533.3480329/1‑94 (MQ=60)

                                                               CAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCA                                <  SRR4302533.2479361/94‑1 (MQ=60)

                                                               CAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCA                                <  SRR4302533.4701350/94‑1 (MQ=60)

                                                               CAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCA                                <  SRR4302533.185015/94‑1 (MQ=60)

                                                               CAGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCA                                <  SRR4302533.3928078/94‑1 (MQ=60)

                                                                AGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAG                               >  SRR4302533.5629454/1‑94 (MQ=60)

                                                                AGTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAG                               >  SRR4302533.2009676/1‑94 (MQ=60)

                                                                 GTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGTAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGC                              >  SRR4302533.5138443/1‑94 (MQ=60)

                                                                 GTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGC                              >  SRR4302533.4192386/1‑94 (MQ=60)

                                                                 GTTTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGC                              <  SRR4302533.1472858/94‑1 (MQ=60)

                                                                   TTGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGT                            >  SRR4302533.1578320/1‑94 (MQ=60)

                                                                    TGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTT                           <  SRR4302533.3920403/94‑1 (MQ=60)

                                                                    TGAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGCT                           >  SRR4302533.1100917/1‑94 (MQ=60)

                                                                     GAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTT                          <  SRR4302533.5848864/94‑1 (MQ=60)

                                                                     GAGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTT                          >  SRR4302533.3774430/1‑94 (MQ=60)

                                                                      AGCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTC                         >  SRR4302533.6097517/1‑94 (MQ=60)

                                                                       GCAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCG                        >  SRR4302533.1858754/1‑94 (MQ=60)

                                                                        CAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGA                       >  SRR4302533.5904739/1‑94 (MQ=60)

                                                                        CAGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGA                       >  SRR4302533.2915589/1‑94 (MQ=60)

                                                                         AGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGAC                      <  SRR4302533.4762657/94‑1 (MQ=60)

                                                                         AGCAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGAC                      >  SRR4302533.799800/1‑94 (MQ=60)

                                                                           CAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGACAG                    <  SRR4302533.1597587/94‑1 (MQ=60)

                                                                           CAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGACAG                    <  SRR4302533.1950613/94‑1 (MQ=60)

                                                                           CAAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGCTGGCGCATCTTCCAGCGTTTCGACAG                    >  SRR4302533.5103354/1‑94 (MQ=60)

                                                                            AAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGccggg                   >  SRR4302533.4758181/1‑89 (MQ=60)

                                                                            AAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGACAGC                   <  SRR4302533.763613/94‑1 (MQ=60)

                                                                            AAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGACAGC                   <  SRR4302533.506037/94‑1 (MQ=60)

                                                                            AAATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGACAGC                   <  SRR4302533.5066234/94‑1 (MQ=60)

                                                                             AATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGACAGCG                  <  SRR4302533.6226159/94‑1 (MQ=60)

                                                                             AATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGACAGCG                  >  SRR4302533.3098671/1‑94 (MQ=60)

                                                                             AATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGACAGCG                  <  SRR4302533.1810222/94‑1 (MQ=60)

                                                                              ATGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGACAGCGG                 <  SRR4302533.3076557/94‑1 (MQ=60)

                                                                               TGGAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTATCAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGACAGCGGC                <  SRR4302533.4471760/94‑1 (MQ=60)

                                                                                 GAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGACAGCGGCAG              <  SRR4302533.3116595/94‑1 (MQ=60)

                                                                                 GAAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGACAGCGGCAG              <  SRR4302533.5060533/94‑1 (MQ=60)

                                                                                  AAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGACAGCGGCAGA             >  SRR4302533.5579647/1‑94 (MQ=60)

                                                                                  AAGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGACAGCGGCAGA             <  SRR4302533.3005618/94‑1 (MQ=60)

                                                                                   AGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGACAGCGGCAGAA            >  SRR4302533.5737705/1‑94 (MQ=60)

                                                                                   AGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGACAGCGGCAGAA            >  SRR4302533.1970487/1‑94 (MQ=60)

                                                                                   AGTCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGACAGCGGCAGAA            >  SRR4302533.5298373/1‑94 (MQ=60)

                                                                                     TCGTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGACAGCGGCAGAATC          >  SRR4302533.4704309/1‑94 (MQ=60)

                                                                                       GTTAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGACAGCGGCAGAATCCT        >  SRR4302533.3601497/1‑94 (MQ=60)

                                                                                         TAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGACAGCGGCAGAATCCTTA      >  SRR4302533.1246803/1‑94 (MQ=60)

                                                                                         TAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGACAGCGGCAGAATCCTTA      <  SRR4302533.5514925/94‑1 (MQ=60)

                                                                                         TAAAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGACAGCGGCAGAATCCTTA      <  SRR4302533.136186/94‑1 (MQ=60)

                                                                                           AAACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGACAGCGGCAGAATCCTTAAA    >  SRR4302533.2958593/1‑94 (MQ=60)

                                                                                            AACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGACAGCGGCAGAATCCTTAAAA   <  SRR4302533.2120251/94‑1 (MQ=60)

                                                                                            AACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGACAGCGGCAGAATCCTTAAAA   >  SRR4302533.3869621/1‑94 (MQ=60)

                                                                                            AACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGACAGCGGCAGAATCCTTAAAA   >  SRR4302533.155095/1‑94 (MQ=60)

                                                                                             ACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGACAGCGGCAGAATCCTTAAAAC  <  SRR4302533.5352936/94‑1 (MQ=60)

                                                                                             ACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGACAGCGGCAGAATCCTTAAAAC  <  SRR4302533.661582/94‑1 (MQ=60)

                                                                                             ACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGACAGCGGCAGAATCCTTAAAAC  >  SRR4302533.5155353/1‑94 (MQ=60)

                                                                                             ACCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGGTTCGACAGCGGCAGCATCCTTAAAAC  >  SRR4302533.5007858/1‑94 (MQ=60)

AATCTGGAAGTCTCCCGTGATGGCGTGGAAAAAGCAGTTACAGCGACAGCGCAGCGTATCGCTCAGTTTGAGCAGCAAATGGAAGTCGTTAAAGCCACTGAAGCCATGCAGCGTGCACAACAGGCTGTAACAACCTCTACCGTTGGCGCATCTTCCAGCGTTTCGACAGCGGCAGAATCCTTAAAAC  >  NC_000913/4408467‑4408653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`