Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,437,861	A→G	V311A (GTG→GCG)	ytfL ←	putative transport protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,437,861	0	A	G	99.6%	759.8 / NA	245	V311A (GTG→GCG)	ytfL	putative transport protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (125/119); minor base T (0/1); total (125/120)
Fisher's exact test for biased strand distribution p-value = 4.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 252 total aligned reads displayed.

AGCCCCTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCACCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGT  >  NC_000913/4437771‑4437950

aGCCCCTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGcc                                                                                         >  2:3781029/1‑93 (MQ=255)

aGCCCCTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGcc                                                                                         >  1:970210/1‑93 (MQ=255)

   cccTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCcgcg                                                                                      >  1:1122924/1‑93 (MQ=255)

    ccTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGa                                                                                     >  2:34728/1‑93 (MQ=255)

     cTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGaa                                                                                    >  1:52207/1‑93 (MQ=255)

        accgaccAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTc                                                                                 <  1:2893413/93‑1 (MQ=255)

         ccgaccAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCt                                                                                <  2:3749164/93‑1 (MQ=255)

           gaccAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTc                                                                              >  1:1517078/1‑93 (MQ=255)

           gaccAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTc                                                                              >  1:5745901/1‑93 (MQ=255)

            accAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCa                                                                             >  2:112958/1‑93 (MQ=255)

            accAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCa                                                                             >  2:5250276/1‑93 (MQ=255)

            accAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCa                                                                             <  2:3581650/93‑1 (MQ=255)

             ccAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCAc                                                                            <  1:3934011/93‑1 (MQ=255)

              cAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCAcc                                                                           <  1:6128042/93‑1 (MQ=255)

               aGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCt                                                                          <  1:1185275/93‑1 (MQ=255)

               aGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCt                                                                          <  1:740026/93‑1 (MQ=255)

               aGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCt                                                                          >  2:664774/1‑93 (MQ=255)

                gATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTg                                                                         >  2:3931645/1‑93 (MQ=255)

                gATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTg                                                                         <  1:773206/93‑1 (MQ=255)

                  tCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCg                                                                       >  2:4602834/1‑93 (MQ=255)

                  tCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCg                                                                       >  2:2548705/1‑93 (MQ=255)

                    gCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGt                                                                     >  1:5031678/1‑93 (MQ=255)

                    ccccATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGt                                                                     <  2:4954797/92‑1 (MQ=255)

                      ccATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCTCCGCGAAGTCTTCACCTGCGGttt                                                                   <  2:4755768/93‑1 (MQ=255)

                      ccATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGttt                                                                   <  2:5503933/93‑1 (MQ=255)

                       cATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGtttt                                                                  >  2:1024535/1‑93 (MQ=255)

                          cAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTaaa                                                               >  2:1435558/1‑93 (MQ=255)

                          cAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTaaa                                                               >  1:3080196/1‑93 (MQ=255)

                            gCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAAc                                                             <  1:4030860/93‑1 (MQ=255)

                              gTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACtt                                                           <  2:3191024/93‑1 (MQ=255)

                               tGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACttt                                                          >  1:2781659/1‑93 (MQ=255)

                                ggTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTc                                                         <  1:404559/93‑1 (MQ=255)

                                ggTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTc                                                         <  1:2064539/93‑1 (MQ=255)

                                  tcatcaCGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCa                                                       >  1:1464355/1‑93 (MQ=255)

                                   catcaCGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCaa                                                      <  1:94635/93‑1 (MQ=255)

                                   catcaCGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCaa                                                      <  2:6035755/93‑1 (MQ=255)

                                    atcaCGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGTTCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAAc                                                     <  1:620251/93‑1 (MQ=255)

                                      caCGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGc                                                   <  2:2094189/93‑1 (MQ=255)

                                       ccGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGcc                                                  <  1:5417670/92‑1 (MQ=255)

                                       aCGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGcc                                                  >  2:1551768/1‑93 (MQ=255)

                                         gTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCctc                                                >  1:5725381/1‑93 (MQ=255)

                                          tCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCctct                                               >  1:1054903/1‑93 (MQ=255)

                                          tCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCctct                                               <  2:1424063/93‑1 (MQ=255)

                                            aTTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGa                                             <  1:3991398/93‑1 (MQ=255)

                                              tGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGaaa                                           <  2:2149747/93‑1 (MQ=255)

                                              tGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGaaa                                           <  2:2882421/93‑1 (MQ=255)

                                              tGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGaaa                                           <  1:330164/93‑1 (MQ=255)

                                                aGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAgg                                         >  1:4721140/1‑93 (MQ=255)

                                                 gggTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAggg                                        <  2:5078081/93‑1 (MQ=255)

                                                  ggTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGt                                       <  1:2158084/93‑1 (MQ=255)

                                                   gTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGtt                                      <  2:5948865/93‑1 (MQ=255)

                                                   gTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGtt                                      <  2:6014001/93‑1 (MQ=255)

                                                    tgatgaTCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTa                                     >  1:673925/1‑93 (MQ=255)

                                                      atgatCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAAc                                   >  1:5914107/1‑93 (MQ=255)

                                                        gatCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGt                                 >  1:183046/1‑93 (MQ=255)

                                                         atCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTa                                <  2:432803/93‑1 (MQ=255)

                                                           ccccACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATc                              <  2:840978/93‑1 (MQ=255)

                                                           ccccACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATc                              <  2:311917/93‑1 (MQ=255)

                                                            cccACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATcc                             <  2:2767592/93‑1 (MQ=255)

                                                            cccACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATcc                             >  2:2717809/1‑93 (MQ=255)

                                                             ccaccaccaGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCg                            >  2:5275379/1‑93 (MQ=255)

                                                             ccaccaccaGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCg                            >  2:1668587/1‑93 (MQ=255)

                                                              caccaccaGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCgg                           >  1:2560602/1‑93 (MQ=255)

                                                               accaccaGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGc                          <  1:5879627/93‑1 (MQ=255)

                                                                ccaccaGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGca                         >  1:5266868/1‑93 (MQ=255)

                                                                  accaGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGcaca                       >  1:4965574/1‑93 (MQ=255)

                                                                  accaGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGcaca                       >  2:778699/1‑93 (MQ=255)

                                                                    caGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAAt                     <  2:4677683/93‑1 (MQ=255)

                                                                     aGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATc                    >  2:4190611/1‑93 (MQ=255)

                                                                     aGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATc                    >  1:3325102/1‑93 (MQ=255)

                                                                      gcgcGTACTCGTTTATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCa                   <  2:2193181/93‑1 (MQ=255)

                                                                      gcgcGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCa                   <  2:5688717/93‑1 (MQ=255)

                                                                       cgcgTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAg                  <  1:5124618/93‑1 (MQ=255)

                                                                       cgcgTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAg                  <  1:1607985/93‑1 (MQ=255)

                                                                        gcgTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGc                 >  1:366097/1‑93 (MQ=255)

                                                                         cgTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCg                >  2:954741/1‑93 (MQ=255)

                                                                          gTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCgt               >  2:3323109/1‑93 (MQ=255)

                                                                          gTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCgt               <  1:4220485/93‑1 (MQ=255)

                                                                           tACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCgtg              >  2:246325/1‑93 (MQ=255)

                                                                           tACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCgtg              >  2:6265973/1‑93 (MQ=255)

                                                                            aCTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCgtgt             <  2:4557931/93‑1 (MQ=255)

                                                                            aCTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCgtgt             <  2:2404479/93‑1 (MQ=255)

                                                                             cTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGtt            <  2:3291630/93‑1 (MQ=255)

                                                                             cTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGtt            <  2:1956740/93‑1 (MQ=255)

                                                                             cTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGtt            <  2:1837481/93‑1 (MQ=255)

                                                                             cTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGtt            <  2:1243727/93‑1 (MQ=255)

                                                                             cTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGtt            >  1:2413393/1‑93 (MQ=255)

                                                                              tCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTg           >  2:5512508/1‑93 (MQ=255)

                                                                               cGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGc          >  1:3433206/1‑93 (MQ=255)

                                                                                 ttCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGa        >  2:5727230/1‑93 (MQ=255)

                                                                                  tCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGaa       <  1:3493095/93‑1 (MQ=255)

                                                                                   cATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAAt      >  1:1911613/1‑93 (MQ=255)

                                                                                   cATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAAt      <  1:5670314/93‑1 (MQ=255)

                                                                                   cATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAAt      <  1:4185783/93‑1 (MQ=255)

                                                                                    aTGATCTCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAAtt     <  1:3132081/93‑1 (MQ=255)

                                                                                    aTGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAAtt     >  2:2277049/1‑93 (MQ=255)

                                                                                      gATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTg   <  2:5842959/93‑1 (MQ=255)

                                                                                      gATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTg   <  1:4897228/93‑1 (MQ=255)

                                                                                       aTCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGt  >  1:4635631/1‑93 (MQ=255)

                                                                                       aTCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGt  <  1:6067566/93‑1 (MQ=255)

AGCCCCTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCACCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGT  >  NC_000913/4437771‑4437950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TCCAGCCCCTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCACCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGTACG  >  NC_000913/4437768‑4437953

TCCAGCCCCTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCG                                                                                              <  SRR4302533.2409350/94‑1 (MQ=60)

  CAGCCCCTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCC                                                                                            >  SRR4302533.975901/1‑94 (MQ=60)

   AGCCCCTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCG                                                                                           >  SRR4302533.2811650/1‑94 (MQ=60)

     CCCCTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCG                                                                                         >  SRR4302533.1129589/1‑94 (MQ=60)

     CCCCTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCG                                                                                         >  SRR4302533.3370884/1‑94 (MQ=60)

     CCCCTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCG                                                                                         >  SRR4302533.5746679/1‑94 (MQ=60)

      CCCTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGA                                                                                        >  SRR4302533.2061927/1‑94 (MQ=60)

       CCTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAA                                                                                       >  SRR4302533.52560/1‑94 (MQ=60)

           ACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCT                                                                                   <  SRR4302533.2911195/94‑1 (MQ=60)

            CCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTT                                                                                  >  SRR4302533.324345/1‑94 (MQ=60)

            CCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTT                                                                                  <  SRR4302533.1887196/94‑1 (MQ=60)

             CGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTC                                                                                 >  SRR4302533.1526212/1‑94 (MQ=60)

             CGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTC                                                                                 >  SRR4302533.5780573/1‑94 (MQ=60)

             CGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTC                                                                                 >  SRR4302533.6328258/1‑94 (MQ=60)

              GACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCA                                                                                >  SRR4302533.4533001/1‑94 (MQ=60)

              GACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCA                                                                                >  SRR4302533.5977336/1‑94 (MQ=60)

               ACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCCCCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCAC                                                                               <  SRR4302533.3905451/94‑1 (MQ=60)

               ACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCAC                                                                               <  SRR4302533.4034454/94‑1 (MQ=60)

                CCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACC                                                                              <  SRR4302533.3958023/94‑1 (MQ=60)

                 CAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCT                                                                             <  SRR4302533.615140/94‑1 (MQ=60)

                 CAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCT                                                                             <  SRR4302533.6164975/94‑1 (MQ=60)

                  AGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTG                                                                            <  SRR4302533.744382/94‑1 (MQ=60)

                  AGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTG                                                                            <  SRR4302533.1192389/94‑1 (MQ=60)

                   GATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGC                                                                           <  SRR4302533.777753/94‑1 (MQ=60)

                     TCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGG                                                                         >  SRR4302533.6218585/1‑94 (MQ=60)

                      CGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGT                                                                        >  SRR4302533.5062143/1‑94 (MQ=60)

                       GCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTT                                                                       >  SRR4302533.3032174/1‑94 (MQ=60)

                       GCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTT                                                                       <  SRR4302533.764499/94‑1 (MQ=60)

                        CCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTT                                                                      <  SRR4302533.4742195/94‑1 (MQ=60)

                          CATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTA                                                                    <  SRR4302533.2777149/94‑1 (MQ=60)

                            TCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAA                                                                  >  SRR4302533.3099094/1‑94 (MQ=60)

                               GCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACT                                                               <  SRR4302533.4220238/94‑1 (MQ=60)

                               GCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACT                                                               <  SRR4302533.4055395/94‑1 (MQ=60)

                                CGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTT                                                              <  SRR4302533.845000/94‑1 (MQ=60)

                                 GTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTT                                                             >  SRR4302533.2798746/1‑94 (MQ=60)

                                 GTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTT                                                             <  SRR4302533.3972735/94‑1 (MQ=60)

                                 GTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTT                                                             >  SRR4302533.3323552/1‑94 (MQ=60)

                                  TGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTC                                                            <  SRR4302533.1186071/94‑1 (MQ=60)

                                  TGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTC                                                            >  SRR4302533.5078981/1‑94 (MQ=60)

                                  TGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTC                                                            <  SRR4302533.50247/94‑1 (MQ=60)

                                   GGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCC                                                           <  SRR4302533.2077265/94‑1 (MQ=60)

                                   GGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCC                                                           <  SRR4302533.406994/94‑1 (MQ=60)

                                    GTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCA                                                          <  SRR4302533.1066832/94‑1 (MQ=60)

                                    GTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCA                                                          <  SRR4302533.4800712/94‑1 (MQ=60)

                                    GTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCA                                                          >  SRR4302533.1473182/1‑94 (MQ=60)

                                     TCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAA                                                         <  SRR4302533.2738693/94‑1 (MQ=60)

                                      CATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAAC                                                        <  SRR4302533.95231/94‑1 (MQ=60)

                                      CATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAAC                                                        <  SRR4302533.545000/94‑1 (MQ=60)

                                      CATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAAC                                                        <  SRR4302533.533797/94‑1 (MQ=60)

                                      CATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAAC                                                        >  SRR4302533.68428/1‑94 (MQ=60)

                                       ATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGTTCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACG                                                       <  SRR4302533.623893/94‑1 (MQ=60)

                                       ATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACG                                                       <  SRR4302533.4063489/94‑1 (MQ=60)

                                         CACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCC                                                     >  SRR4302533.5714936/1‑94 (MQ=60)

                                         CACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCC                                                     >  SRR4302533.2200596/1‑94 (MQ=60)

                                          CCGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCT                                                    <  SRR4302533.5450260/94‑1 (MQ=60)

                                           CGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTC                                                   >  SRR4302533.5759946/1‑94 (MQ=60)

                                            GTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCT                                                  >  SRR4302533.1061110/1‑94 (MQ=60)

                                            GTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCT                                                  >  SRR4302533.1004978/1‑94 (MQ=60)

                                               ATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAA                                               <  SRR4302533.3985612/94‑1 (MQ=60)

                                               ATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAA                                               <  SRR4302533.4015706/94‑1 (MQ=60)

                                               ATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAA                                               >  SRR4302533.955994/1‑94 (MQ=60)

                                                 TGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAG                                             <  SRR4302533.332182/94‑1 (MQ=60)

                                                  GAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGG                                            >  SRR4302533.4749765/1‑94 (MQ=60)

                                                  GAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGG                                            <  SRR4302533.5935989/94‑1 (MQ=60)

                                                   AGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGG                                           <  SRR4302533.1160466/94‑1 (MQ=60)

                                                    GGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGT                                          >  SRR4302533.5851208/1‑94 (MQ=60)

                                                     GGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTT                                         <  SRR4302533.2171369/94‑1 (MQ=60)

                                                      GTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTA                                        >  SRR4302533.677903/1‑94 (MQ=60)

                                                       TGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAA                                       <  SRR4302533.5009425/94‑1 (MQ=60)

                                                        GATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAAC                                      <  SRR4302533.3674856/94‑1 (MQ=60)

                                                        GATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAAC                                      <  SRR4302533.3131566/94‑1 (MQ=60)

                                                        GATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAAC                                      <  SRR4302533.476523/94‑1 (MQ=60)

                                                        GATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAAC                                      >  SRR4302533.5949847/1‑94 (MQ=60)

                                                         ATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACG                                     <  SRR4302533.5368178/94‑1 (MQ=60)

                                                         ATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACG                                     <  SRR4302533.798966/94‑1 (MQ=60)

                                                          TGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGT                                    >  SRR4302533.184182/1‑94 (MQ=60)

                                                          TGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGT                                    <  SRR4302533.4106014/94‑1 (MQ=60)

                                                           GATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTA                                   >  SRR4302533.4953527/1‑94 (MQ=60)

                                                            ATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTAT                                  >  SRR4302533.1945299/1‑94 (MQ=60)

                                                             TCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATC                                 >  SRR4302533.4064933/1‑94 (MQ=60)

                                                               CCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCG                               <  SRR4302533.5164600/94‑1 (MQ=60)

                                                               CCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCG                               >  SRR4302533.992866/1‑94 (MQ=60)

                                                                CCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGG                              >  SRR4302533.2576372/1‑94 (MQ=60)

                                                                  ACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCA                            >  SRR4302533.1808876/1‑94 (MQ=60)

                                                                  ACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCA                            >  SRR4302533.1604774/1‑94 (MQ=60)

                                                                  ACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCA                            <  SRR4302533.5915162/94‑1 (MQ=60)

                                                                  ACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCA                            >  SRR4302533.5298469/1‑94 (MQ=60)

                                                                    CACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACA                          >  SRR4302533.4995675/1‑94 (MQ=60)

                                                                    CACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACA                          >  SRR4302533.1367403/1‑94 (MQ=60)

                                                                       CAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATC                       >  SRR4302533.3345646/1‑94 (MQ=60)

                                                                        AGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCA                      >  SRR4302533.6196645/1‑94 (MQ=60)

                                                                        AGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCA                      <  SRR4302533.2952694/94‑1 (MQ=60)

                                                                        AGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCA                      <  SRR4302533.1482235/94‑1 (MQ=60)

                                                                         GCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAG                     <  SRR4302533.5998916/94‑1 (MQ=60)

                                                                          CGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGC                    <  SRR4302533.1617741/94‑1 (MQ=60)

                                                                          CGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGC                    >  SRR4302533.368314/1‑94 (MQ=60)

                                                                          CGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGC                    <  SRR4302533.5155575/94‑1 (MQ=60)

                                                                           GCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCG                   <  SRR4302533.699128/94‑1 (MQ=60)

                                                                            CGTACACGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGT                  >  SRR4302533.1675585/1‑94 (MQ=60)

                                                                             GTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTG                 <  SRR4302533.4246008/94‑1 (MQ=60)

                                                                               ACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTT               >  SRR4302533.2428303/1‑94 (MQ=60)

                                                                                CTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTG              <  SRR4302533.5162815/94‑1 (MQ=60)

                                                                                CTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTG              >  SRR4302533.5842238/1‑94 (MQ=60)

                                                                                 TCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAccgggttgg             >  SRR4302533.2536639/1‑85 (MQ=60)

                                                                                 TCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGC             >  SRR4302533.3454416/1‑94 (MQ=60)

                                                                                 TCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGC             >  SRR4302533.1496995/1‑94 (MQ=60)

                                                                                 TCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGC             >  SRR4302533.3192359/1‑94 (MQ=60)

                                                                                  CGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCG            >  SRR4302533.3666341/1‑94 (MQ=60)

                                                                                  CGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCG            <  SRR4302533.3459898/94‑1 (MQ=60)

                                                                                   GTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGCGTTGCGA           >  SRR4302533.2202185/1‑94 (MQ=60)

                                                                                    TTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAA          <  SRR4302533.2130637/94‑1 (MQ=60)

                                                                                    TTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAA          >  SRR4302533.529669/1‑94 (MQ=60)

                                                                                     TCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAAT         <  SRR4302533.3514638/94‑1 (MQ=60)

                                                                                     TCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAAT         >  SRR4302533.1923276/1‑94 (MQ=60)

                                                                                      CATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATT        <  SRR4302533.4211128/94‑1 (MQ=60)

                                                                                      CATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATT        <  SRR4302533.5704561/94‑1 (MQ=60)

                                                                                       ATGATCTCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTT       <  SRR4302533.3151339/94‑1 (MQ=60)

                                                                                       ATGAGCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTGTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTT       <  SRR4302533.3375802/94‑1 (MQ=60)

                                                                                        TGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTG      >  SRR4302533.4393357/1‑94 (MQ=60)

                                                                                        TGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTG      <  SRR4302533.4083047/94‑1 (MQ=60)

                                                                                         GATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGT     <  SRR4302533.4926902/94‑1 (MQ=60)

                                                                                         GATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGT     >  SRR4302533.4663719/1‑94 (MQ=60)

                                                                                          ATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAGCTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGTA    <  SRR4302533.5777456/94‑1 (MQ=60)

                                                                                          ATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGTA    <  SRR4302533.6104283/94‑1 (MQ=60)

                                                                                          ATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGTA    >  SRR4302533.4744017/1‑94 (MQ=60)

                                                                                           TCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGTAC   <  SRR4302533.1781197/94‑1 (MQ=60)

                                                                                           TCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGTAC   <  SRR4302533.3418047/94‑1 (MQ=60)

                                                                                           TCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGTAC   <  SRR4302533.5775435/94‑1 (MQ=60)

                                                                                           TCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGTAC   <  SRR4302533.2389847/94‑1 (MQ=60)

                                                                                           TCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGTAC   <  SRR4302533.5258071/94‑1 (MQ=60)

                                                                                            CGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGGTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGTACG  >  SRR4302533.445826/1‑94 (MQ=60)

TCCAGCCCCTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCACCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGTACG  >  NC_000913/4437768‑4437953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`