Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,437,861 A→G V311A (GTG→GCG)  ytfL ← putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,437,8610AG99.2% 1096.4 / ‑5.8 364V311A (GTG→GCG) ytfLputative transport protein
Reads supporting (aligned to +/- strand):  ref base A (1/2);  new base G (215/143);  total (217/147)
Fisher's exact test for biased strand distribution p-value = 5.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 378 total aligned reads displayed.

AGCCCCTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCACCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGT  >  NC_000913/4437771‑4437950
                                                                                          |                                                                                         
 gCCCCTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCcg                                                                                        >  1:8288555/1‑93 (MQ=255)
    ccTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCgaga                                                                                     >  1:5064524/1‑93 (MQ=255)
     cTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGaa                                                                                    <  1:6753636/93‑1 (MQ=255)
     cTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGaa                                                                                    <  2:8866459/93‑1 (MQ=255)
     cTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGaa                                                                                    >  1:4296836/1‑93 (MQ=255)
       gaccgaccAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGt                                                                                  >  2:8427671/1‑93 (MQ=255)
        accgaccAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAggc                                                                                 >  1:223970/1‑91 (MQ=255)
        accgaccAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTc                                                                                 >  2:9230276/1‑93 (MQ=255)
        accgaccAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCACCGCGAAGTc                                                                                 >  2:5321049/1‑93 (MQ=255)
           gaccAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTc                                                                              <  2:1919550/93‑1 (MQ=255)
            accAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCa                                                                             >  2:3137645/1‑93 (MQ=255)
            accAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCa                                                                             >  2:1467354/1‑93 (MQ=255)
            accAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCa                                                                             >  1:8229758/1‑93 (MQ=255)
            accAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCa                                                                             >  1:2527247/1‑93 (MQ=255)
              cAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGACGTCTTCAcc                                                                           >  2:7129750/1‑93 (MQ=255)
              cAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCAcc                                                                           <  1:1452757/93‑1 (MQ=255)
              cAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCAcc                                                                           <  1:5990622/93‑1 (MQ=255)
               aGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCt                                                                          <  2:3836010/93‑1 (MQ=255)
               aGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCt                                                                          >  1:835300/1‑93 (MQ=255)
               aGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCt                                                                          <  2:6526799/93‑1 (MQ=255)
                gATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTg                                                                         >  2:4288969/1‑93 (MQ=255)
                 aTCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGc                                                                        >  2:7455648/1‑93 (MQ=255)
                  tCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCg                                                                       <  1:9795795/93‑1 (MQ=255)
                   cGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCgg                                                                      <  2:3943380/93‑1 (MQ=255)
                      ccATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGttt                                                                   <  1:3671041/93‑1 (MQ=255)
                       cATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGtttt                                                                  >  2:8582830/1‑93 (MQ=255)
                       cATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGtttt                                                                  >  2:6308060/1‑93 (MQ=255)
                       cATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGtttt                                                                  >  1:1605630/1‑93 (MQ=255)
                        aTCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTa                                                                 <  1:1343460/93‑1 (MQ=255)
                        aTCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTa                                                                 >  1:2755843/1‑93 (MQ=255)
                        aTCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTAACCTGCGGTTTTa                                                                 >  1:3211154/1‑93 (MQ=255)
                         tCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTaa                                                                >  2:7834237/1‑93 (MQ=255)
                         tCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTaa                                                                >  1:9303494/1‑93 (MQ=255)
                          cAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTaaa                                                               >  2:8351626/1‑93 (MQ=255)
                           aGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTaaaa                                                              >  1:1235402/1‑93 (MQ=255)
                           aGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTaaaa                                                              <  1:4738650/93‑1 (MQ=255)
                             cGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACt                                                            <  1:934448/93‑1 (MQ=255)
                              gTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACtt                                                           <  1:1893101/93‑1 (MQ=255)
                              gTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACtt                                                           >  2:2323106/1‑93 (MQ=255)
                                 gTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTcc                                                        >  1:8459575/1‑93 (MQ=255)
                                 gTCATCACGTCATTGAGGGTGATCATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTcc                                                        <  2:5580638/93‑1 (MQ=255)
                                  tcatcaCGTCATTGAGGGTGATGATCCCCTCCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCa                                                       <  1:4124263/93‑1 (MQ=255)
                                  tcatcaCGCCATTGAGGGTGATGATCCCCCCCACCAGCGCGTACAAGTTCATGATCACCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCa                                                       <  2:5809991/93‑1 (MQ=255)
                                   catcaCGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCTCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCaa                                                      <  1:9263211/93‑1 (MQ=255)
                                   catcaCGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCaa                                                      <  1:796185/93‑1 (MQ=255)
                                   catcaCGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCaa                                                      >  1:6603929/1‑93 (MQ=255)
                                      caCGTCATTGAGGGTGATGATCCCCACCGCCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGc                                                   <  1:5544239/93‑1 (MQ=255)
                                      caCGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGc                                                   <  1:337453/93‑1 (MQ=255)
                                      caCGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGc                                                   >  2:1261063/1‑93 (MQ=255)
                                      caCGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGc                                                   >  1:5433126/1‑93 (MQ=255)
                                      caCGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGc                                                   <  1:8603357/93‑1 (MQ=255)
                                         gTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCctc                                                >  1:7336900/1‑93 (MQ=255)
                                         gTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCctc                                                >  2:4913757/1‑93 (MQ=255)
                                          tCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCctct                                               >  2:5231040/1‑93 (MQ=255)
                                          tCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCctct                                               >  1:185562/1‑93 (MQ=255)
                                          tCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCctct                                               <  2:2198673/93‑1 (MQ=255)
                                           cATTGAGGGCAATCATCCCCACCACCAGCGGGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTg                                              <  2:2552511/93‑1 (MQ=255)
                                            aTTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGa                                             >  2:9685825/1‑93 (MQ=255)
                                            aTTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGa                                             >  1:5171927/1‑93 (MQ=255)
                                             ttGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGaa                                            >  1:908754/1‑93 (MQ=255)
                                              tGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGaaa                                           >  2:5065982/1‑93 (MQ=255)
                                              tGAGGGTGAGGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCGGCGGTTTTAAAACTTTCCAACGCCTCTGaaa                                           <  1:3694845/93‑1 (MQ=255)
                                                aGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAgg                                         >  2:9435509/1‑93 (MQ=255)
                                                aGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAgg                                         >  2:9784196/1‑93 (MQ=255)
                                                aGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAgg                                         <  1:6742615/93‑1 (MQ=255)
                                                 gggTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAggg                                        >  1:5823768/1‑93 (MQ=255)
                                                   gTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGtt                                      <  1:9580472/93‑1 (MQ=255)
                                                    tgatgaTCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTa                                     <  1:9482945/93‑1 (MQ=255)
                                                     gatgatCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTaa                                    <  1:4471950/93‑1 (MQ=255)
                                                       tgatCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCCCCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACg                                  <  2:7584646/93‑1 (MQ=255)
                                                        gatCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGt                                 >  2:677791/1‑93 (MQ=255)
                                                          tCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTAt                               >  1:6355678/1‑93 (MQ=255)
                                                           ccccACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATc                              >  2:2653945/1‑93 (MQ=255)
                                                           ccccACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATc                              <  2:8741221/93‑1 (MQ=255)
                                                              caccaccaGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCgg                           <  2:9547749/93‑1 (MQ=255)
                                                              caccaccaGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCgg                           >  2:6146812/1‑93 (MQ=255)
                                                                ccaccaGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGca                         >  2:10037948/1‑93 (MQ=255)
                                                                ccaccaGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGca                         >  2:3112066/1‑93 (MQ=255)
                                                                 caccaGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGcac                        >  1:4047835/1‑93 (MQ=255)
                                                                   ccaGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACaa                      >  1:5719213/1‑93 (MQ=255)
                                                                   ccaGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACaa                      >  1:4654425/1‑93 (MQ=255)
                                                                     aGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATc                    <  1:7647831/93‑1 (MQ=255)
                                                                     aGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATc                    <  2:7314061/93‑1 (MQ=255)
                                                                      gcgcGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCa                   <  2:6666240/93‑1 (MQ=255)
                                                                         cgTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCg                <  2:370679/93‑1 (MQ=255)
                                                                          gTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCgt               <  2:478814/93‑1 (MQ=255)
                                                                           tACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCgtg              >  2:1694567/1‑93 (MQ=255)
                                                                            aCTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCgtgt             >  2:1165296/1‑93 (MQ=255)
                                                                             cTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCTGGCACAATCAGCGTGtt            >  1:2089365/1‑93 (MQ=255)
                                                                             cTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGtt            <  2:4640887/93‑1 (MQ=255)
                                                                             cTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATACGGCACAATCAGCGTGtt            >  2:3226565/1‑93 (MQ=255)
                                                                              tCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTg           >  1:30768/1‑93 (MQ=255)
                                                                               cGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGc          <  2:2807165/93‑1 (MQ=255)
                                                                                 ttCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGa        <  1:8664972/93‑1 (MQ=255)
                                                                                 ttCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAAAGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGa        >  2:2067556/1‑93 (MQ=255)
                                                                                 ttCATGATCGCCGCGAAGTCTTCACCTGCGGGTTTAAAACTTTCCAACGCCTCTGAAAAGGTTAACGTATCCGGCACAATCAGCGTGTTGCGa        >  2:3413511/1‑93 (MQ=255)
                                                                                  tCATGATCTCCGCGAAGTCTTCACCTGCGGTTTTAAAGCTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGaa       <  2:793293/93‑1 (MQ=255)
                                                                                  tCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGaa       <  1:4248971/93‑1 (MQ=255)
                                                                                     tGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAAttt    >  1:6965853/1‑93 (MQ=255)
                                                                                     tGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAAttt    >  2:2964527/1‑93 (MQ=255)
                                                                                          |                                                                                         
AGCCCCTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCACCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGT  >  NC_000913/4437771‑4437950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 203 total aligned reads displayed.
TCCAGCCCCTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCACCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGTACGC  >  NC_000913/4437768‑4437954
                                                                                             |                                                                                             
TCCAGCCCCTGACCGACCAGATCGCCCATGAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCG                                                                                               <  SRR4302540.1063912/94‑1 (MQ=60)
TCCAGCCCCTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCG                                                                                               >  SRR4302540.3987473/1‑94 (MQ=60)
  CAGCCCCTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCC                                                                                             <  SRR4302540.772324/94‑1 (MQ=60)
  CAGCCCCTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCC                                                                                             <  SRR4302540.9896338/94‑1 (MQ=60)
  CAGCCCCTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCC                                                                                             >  SRR4302540.1524760/1‑94 (MQ=60)
   AGCCCCTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCG                                                                                            >  SRR4302540.8384811/1‑94 (MQ=60)
    GCCCCTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGC                                                                                           <  SRR4302540.409780/94‑1 (MQ=60)
     CCCCTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCG                                                                                          <  SRR4302540.8415825/94‑1 (MQ=60)
      CCCTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGAGA                                                                                         >  SRR4302540.5119281/1‑94 (MQ=60)
       CCTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAA                                                                                        <  SRR4302540.4734059/94‑1 (MQ=60)
       CCTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAA                                                                                        >  SRR4302540.3917824/1‑94 (MQ=60)
       CCTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAA                                                                                        >  SRR4302540.4343595/1‑94 (MQ=60)
       CCTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGCGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAA                                                                                        >  SRR4302540.9675628/1‑94 (MQ=60)
        CTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAG                                                                                       <  SRR4302540.6828262/94‑1 (MQ=60)
        CTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAG                                                                                       <  SRR4302540.1855543/94‑1 (MQ=60)
          GACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTC                                                                                     >  SRR4302540.9114225/1‑94 (MQ=60)
          GACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGGC                                                                                     >  SRR4302540.226137/1‑94 (MQ=60)
           ACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCT                                                                                    >  SRR4302540.1678583/1‑94 (MQ=60)
            CCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTT                                                                                   >  SRR4302540.5944428/1‑94 (MQ=60)
             CGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTC                                                                                  >  SRR4302540.1884875/1‑94 (MQ=60)
             CGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTC                                                                                  <  SRR4302540.5820730/94‑1 (MQ=60)
              GACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCA                                                                                 >  SRR4302540.8325422/1‑94 (MQ=60)
              GACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCA                                                                                 <  SRR4302540.6521342/94‑1 (MQ=60)
              GACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCA                                                                                 >  SRR4302540.8325421/1‑94 (MQ=60)
              GACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCA                                                                                 >  SRR4302540.2554521/1‑94 (MQ=60)
              GACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCA                                                                                 >  SRR4302540.5350672/1‑94 (MQ=60)
                CCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGTGAAGTCTTCACC                                                                               <  SRR4302540.380489/94‑1 (MQ=60)
                CCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACC                                                                               <  SRR4302540.8262015/94‑1 (MQ=60)
                CCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACC                                                                               >  SRR4302540.803805/1‑94 (MQ=60)
                 CAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCT                                                                              >  SRR4302540.2144779/1‑94 (MQ=60)
                 CAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCT                                                                              <  SRR4302540.1468559/94‑1 (MQ=60)
                 CAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCT                                                                              <  SRR4302540.6056989/94‑1 (MQ=60)
                 CAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCT                                                                              >  SRR4302540.844170/1‑94 (MQ=60)
                 CAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGGGAGGATCCCCACCCCCAGCGCGCACTCGTTCATGATCGCCGCGAcggcgtcccct                                                                              >  SRR4302540.844171/1‑83 (MQ=60)
                  AGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTG                                                                             <  SRR4302540.9554179/94‑1 (MQ=60)
                   GATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGC                                                                            >  SRR4302540.2039035/1‑94 (MQ=60)
                   GATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGC                                                                            >  SRR4302540.282394/1‑94 (MQ=60)
                    ATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGTTCGCCGCGAAGTCTTCACCTGCG                                                                           >  SRR4302540.8752085/1‑94 (MQ=60)
                    ATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCG                                                                           >  SRR4302540.2566140/1‑94 (MQ=60)
                    ATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCG                                                                           >  SRR4302540.6801526/1‑94 (MQ=60)
                     TCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGG                                                                          <  SRR4302540.9910526/94‑1 (MQ=60)
                       agCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTT                                                                        <  SRR4302540.8753992/92‑1 (MQ=60)
                       GCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTT                                                                        <  SRR4302540.4610743/94‑1 (MQ=60)
                       GCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTT                                                                        >  SRR4302540.8998485/1‑94 (MQ=60)
                        CCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTT                                                                       <  SRR4302540.4119243/94‑1 (MQ=60)
                         CCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTT                                                                      >  SRR4302540.1623202/1‑94 (MQ=60)
                         CCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTT                                                                      <  SRR4302540.3711194/94‑1 (MQ=60)
                          CATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTA                                                                     <  SRR4302540.3242076/94‑1 (MQ=60)
                          CATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTA                                                                     >  SRR4302540.2785628/1‑94 (MQ=60)
                          CATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTA                                                                     >  SRR4302540.5494753/1‑94 (MQ=60)
                          CATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTA                                                                     >  SRR4302540.4245305/1‑94 (MQ=60)
                          CATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTAACCTGCGGTTTTA                                                                     >  SRR4302540.3246012/1‑94 (MQ=60)
                           ATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAA                                                                    <  SRR4302540.1357549/94‑1 (MQ=60)
                           ATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAA                                                                    >  SRR4302540.9414618/1‑94 (MQ=60)
                           ATCAGCGTGGTCATCACGACATTGAGGGAGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCTGTTTTAA                                                                    >  SRR4302540.4722973/1‑94 (MQ=60)
                             CAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAA                                                                  >  SRR4302540.1248021/1‑94 (MQ=60)
                             CAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAA                                                                  >  SRR4302540.8709850/1‑94 (MQ=60)
                             CAGCGGGGTCATCACGTCAGTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAA                                                                  <  SRR4302540.5443321/94‑1 (MQ=60)
                              AGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAAC                                                                 >  SRR4302540.5225157/1‑94 (MQ=60)
                              AGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAAC                                                                 <  SRR4302540.4789227/94‑1 (MQ=60)
                              AGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAAC                                                                 <  SRR4302540.210732/94‑1 (MQ=60)
                               GCGTGGTCATCACGCCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACT                                                                >  SRR4302540.6792333/1‑94 (MQ=60)
                                CGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTT                                                               >  SRR4302540.333079/1‑94 (MQ=60)
                                CGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTT                                                               <  SRR4302540.944247/94‑1 (MQ=60)
                                CGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTT                                                               >  SRR4302540.7253733/1‑94 (MQ=60)
                                 GTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTT                                                              >  SRR4302540.6162042/1‑94 (MQ=60)
                                 GTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTT                                                              <  SRR4302540.1913821/94‑1 (MQ=60)
                                   GGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCC                                                            >  SRR4302540.8557795/1‑94 (MQ=60)
                                   GGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCC                                                            <  SRR4302540.9784476/94‑1 (MQ=60)
                                    GTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCA                                                           <  SRR4302540.4308387/94‑1 (MQ=60)
                                    GTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCA                                                           <  SRR4302540.8586892/94‑1 (MQ=60)
                                    GTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCA                                                           <  SRR4302540.1868580/94‑1 (MQ=60)
                                    GTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCA                                                           <  SRR4302540.7666673/94‑1 (MQ=60)
                                    GTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCA                                                           <  SRR4302540.9767862/94‑1 (MQ=60)
                                     TCATCACGTCATTGAGGGTGATGATCCCCTCCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAA                                                          <  SRR4302540.4168807/94‑1 (MQ=60)
                                     TCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAA                                                          >  SRR4302540.6677057/1‑94 (MQ=60)
                                      CATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCTCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAAC                                                         <  SRR4302540.9374021/94‑1 (MQ=60)
                                      CATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAAC                                                         <  SRR4302540.9374022/94‑1 (MQ=60)
                                      CATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAAC                                                         <  SRR4302540.3714191/94‑1 (MQ=60)
                                      CATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAAC                                                         >  SRR4302540.3716467/1‑94 (MQ=60)
                                      CATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAAC                                                         <  SRR4302540.804642/94‑1 (MQ=60)
                                      CATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAAC                                                         >  SRR4302540.1529246/1‑94 (MQ=60)
                                      CATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAAC                                                         <  SRR4302540.156998/94‑1 (MQ=60)
                                      CATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAAC                                                         <  SRR4302540.4657590/94‑1 (MQ=60)
                                      CATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAAC                                                         <  SRR4302540.5978007/94‑1 (MQ=60)
                                       ATCACGTCATTGAGGGTGATGATCCCCAACACCAGCGCGCACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTCAAACTTTCAAACG                                                        >  SRR4302540.9503775/1‑94 (MQ=60)
                                        cccCGTCAATGAGGGTGATGATCCCCACCACCAGCGCGCCCTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGC                                                       <  SRR4302540.5694588/91‑1 (MQ=60)
                                        TCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGC                                                       >  SRR4302540.5492882/1‑94 (MQ=60)
                                        TCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGC                                                       <  SRR4302540.40911/94‑1 (MQ=60)
                                        TCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCACCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGC                                                       <  SRR4302540.5694589/94‑1 (MQ=60)
                                         CACGTCATTGAGGGTGATGATCCCCACCGCCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCC                                                      <  SRR4302540.5605312/94‑1 (MQ=60)
                                         CACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCC                                                      >  SRR4302540.2158825/1‑94 (MQ=60)
                                         CACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCC                                                      >  SRR4302540.6432612/1‑94 (MQ=60)
                                         CACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCC                                                      >  SRR4302540.5714938/1‑94 (MQ=60)
                                         CACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCC                                                      <  SRR4302540.8703345/94‑1 (MQ=60)
                                         CACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCC                                                      <  SRR4302540.340856/94‑1 (MQ=60)
                                         CACGTCATTGAGGGTGATGACCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCC                                                      <  SRR4302540.2629916/94‑1 (MQ=60)
                                          ACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCT                                                     >  SRR4302540.4297686/1‑94 (MQ=60)
                                           CGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTC                                                    >  SRR4302540.7418782/1‑94 (MQ=60)
                                            GTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCT                                                   >  SRR4302540.187407/1‑94 (MQ=60)
                                            GTCATTCAGGGTGATGATCCCCCCCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCT                                                   <  SRR4302540.849377/94‑1 (MQ=60)
                                             TCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTG                                                  >  SRR4302540.9744036/1‑94 (MQ=60)
                                              CATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGA                                                 >  SRR4302540.2351195/1‑94 (MQ=60)
                                              CATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGA                                                 >  SRR4302540.3366401/1‑94 (MQ=60)
                                              CATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGA                                                 >  SRR4302540.9232758/1‑94 (MQ=60)
                                              CATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGA                                                 >  SRR4302540.9232760/1‑94 (MQ=60)
                                              CATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGA                                                 >  SRR4302540.5228212/1‑94 (MQ=60)
                                               ATTGAGGGTGATGATCCCCACCGCCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAA                                                <  SRR4302540.9447030/94‑1 (MQ=60)
                                               ATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAA                                                >  SRR4302540.344971/1‑94 (MQ=60)
                                               ATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAA                                                >  SRR4302540.918390/1‑94 (MQ=60)
                                                TTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAA                                               >  SRR4302540.2111416/1‑94 (MQ=60)
                                                TTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAA                                               >  SRR4302540.4806857/1‑94 (MQ=60)
                                                TTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAA                                               <  SRR4302540.8828539/94‑1 (MQ=60)
                                                 TGAGGGTGAGGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCGGCGGTTTTAAAACTTTCCAACGCCTCTGAAAG                                              <  SRR4302540.3735191/94‑1 (MQ=60)
                                                  GAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGG                                             <  SRR4302540.3495855/94‑1 (MQ=60)
                                                  GAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGG                                             >  SRR4302540.9400775/1‑94 (MQ=60)
                                                   AGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGG                                            >  SRR4302540.5888143/1‑94 (MQ=60)
                                                   AGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGG                                            <  SRR4302540.6817110/94‑1 (MQ=60)
                                                   AGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCGGCGGTTTTAAAACTTTCCACCGCCTCTGAAcggt                                            >  SRR4302540.3297029/1‑90 (MQ=60)
                                                    GGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGT                                           >  SRR4302540.5024365/1‑94 (MQ=60)
                                                    GGGTGATGACCCCCACCTCCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGT                                           <  SRR4302540.8340021/94‑1 (MQ=60)
                                                     GGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTT                                          <  SRR4302540.2743849/94‑1 (MQ=60)
                                                     GGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTT                                          >  SRR4302540.347614/1‑94 (MQ=60)
                                                      GTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTA                                         <  SRR4302540.9693603/94‑1 (MQ=60)
                                                      GTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTA                                         <  SRR4302540.4508968/94‑1 (MQ=60)
                                                       TGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAA                                        >  SRR4302540.2266153/1‑94 (MQ=60)
                                                       TGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAA                                        >  SRR4302540.5176203/1‑94 (MQ=60)
                                                       TGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAA                                        <  SRR4302540.9595387/94‑1 (MQ=60)
                                                        GATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAAC                                       <  SRR4302540.4520451/94‑1 (MQ=60)
                                                        GATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAAC                                       >  SRR4302540.9511413/1‑94 (MQ=60)
                                                        GATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAAC                                       <  SRR4302540.5877563/94‑1 (MQ=60)
                                                         ATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACG                                      <  SRR4302540.8198992/94‑1 (MQ=60)
                                                           TATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTA                                    <  SRR4302540.3147358/94‑1 (MQ=60)
                                                           GATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTA                                    <  SRR4302540.4983631/94‑1 (MQ=60)
                                                           GATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCAAACGCCTCTGGAAGGGTTAACGTA                                    >  SRR4302540.7035778/1‑94 (MQ=60)
                                                            ATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTAT                                   >  SRR4302540.739544/1‑94 (MQ=60)
                                                            ATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTAT                                   >  SRR4302540.6426452/1‑94 (MQ=60)
                                                            ATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTAT                                   >  SRR4302540.8410924/1‑94 (MQ=60)
                                                              CCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCC                                 <  SRR4302540.2839912/94‑1 (MQ=60)
                                                               CCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCG                                >  SRR4302540.8439914/1‑94 (MQ=60)
                                                               CCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCG                                >  SRR4302540.8522141/1‑94 (MQ=60)
                                                               CCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCG                                >  SRR4302540.6348717/1‑94 (MQ=60)
                                                               CCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGGATCCG                                >  SRR4302540.8522142/1‑94 (MQ=60)
                                                                CCACCACCAGCGCGTACTCGTTCGTGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGG                               >  SRR4302540.6832386/1‑94 (MQ=60)
                                                                  CCCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCA                             <  SRR4302540.5815544/94‑1 (MQ=60)
                                                                  ACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCA                             >  SRR4302540.9256945/1‑94 (MQ=60)
                                                                   CCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCAC                            >  SRR4302540.4091479/1‑94 (MQ=60)
                                                                   CCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCAC                            >  SRR4302540.1321211/1‑94 (MQ=60)
                                                                   CCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACATATCCGGCAC                            >  SRR4302540.9948555/1‑94 (MQ=60)
                                                                    CACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACA                           >  SRR4302540.4660215/1‑94 (MQ=60)
                                                                     ACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAA                          >  SRR4302540.7830089/1‑94 (MQ=60)
                                                                     ACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAA                          >  SRR4302540.4704232/1‑94 (MQ=60)
                                                                     ACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAA                          >  SRR4302540.5782164/1‑94 (MQ=60)
                                                                       CAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATC                        >  SRR4302540.7901681/1‑94 (MQ=60)
                                                                       CAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATC                        >  SRR4302540.10114145/1‑94 (MQ=60)
                                                                        AGCGCGTCCTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCA                       <  SRR4302540.4738878/94‑1 (MQ=60)
                                                                        AGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCA                       <  SRR4302540.141714/94‑1 (MQ=60)
                                                                        AGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCA                       <  SRR4302540.3920329/94‑1 (MQ=60)
                                                                        AGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCA                       >  SRR4302540.7612175/1‑94 (MQ=60)
                                                                        AGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCA                       <  SRR4302540.1373937/94‑1 (MQ=60)
                                                                        AGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCA                       <  SRR4302540.1373936/94‑1 (MQ=60)
                                                                        AGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCA                       <  SRR4302540.7733921/94‑1 (MQ=60)
                                                                         GCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAG                      >  SRR4302540.9672852/1‑94 (MQ=60)
                                                                         GCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAG                      <  SRR4302540.2200296/94‑1 (MQ=60)
                                                                           GCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCG                    >  SRR4302540.4218019/1‑94 (MQ=60)
                                                                            CGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGT                   <  SRR4302540.7688867/94‑1 (MQ=60)
                                                                            CGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGT                   >  SRR4302540.6520311/1‑94 (MQ=60)
                                                                            CGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGT                   <  SRR4302540.9447147/94‑1 (MQ=60)
                                                                            CGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGT                   <  SRR4302540.1909048/94‑1 (MQ=60)
                                                                             GTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCGACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTG                  >  SRR4302540.631196/1‑94 (MQ=60)
                                                                             GTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTG                  <  SRR4302540.6710429/94‑1 (MQ=60)
                                                                             GTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTG                  >  SRR4302540.6823559/1‑94 (MQ=60)
                                                                             GTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTG                  >  SRR4302540.6823855/1‑94 (MQ=60)
                                                                             GTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTG                  >  SRR4302540.1277313/1‑94 (MQ=60)
                                                                               ACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCTGGCACAATCAGCGTGTT                >  SRR4302540.2111811/1‑94 (MQ=60)
                                                                               ACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTT                <  SRR4302540.4389022/94‑1 (MQ=60)
                                                                               ACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTT                <  SRR4302540.937414/94‑1 (MQ=60)
                                                                                CTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTG               <  SRR4302540.4312599/94‑1 (MQ=60)
                                                                                CTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTG               >  SRR4302540.31093/1‑94 (MQ=60)
                                                                                 TCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGC              >  SRR4302540.8680053/1‑94 (MQ=60)
                                                                                   GTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGA            >  SRR4302540.2391199/1‑94 (MQ=60)
                                                                                   GTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGA            >  SRR4302540.5564379/1‑94 (MQ=60)
                                                                                   GTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGA            >  SRR4302540.9623599/1‑94 (MQ=60)
                                                                                    TTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAA           <  SRR4302540.8765615/94‑1 (MQ=60)
                                                                                     TCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAAT          <  SRR4302540.4295201/94‑1 (MQ=60)
                                                                                     TCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAAT          >  SRR4302540.7023427/1‑94 (MQ=60)
                                                                                       ATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTT        <  SRR4302540.8340327/94‑1 (MQ=60)
                                                                                       ATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTT        >  SRR4302540.5534091/1‑94 (MQ=60)
                                                                                       ATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTT        >  SRR4302540.7043307/1‑94 (MQ=60)
                                                                                        TGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTG       >  SRR4302540.5250963/1‑94 (MQ=60)
                                                                                         GATCGCTGCGAAGTCTTCACCTGCGGTTTTTAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGTATAATCAGCGTGTTGCGAATTTGT      >  SRR4302540.9081627/1‑94 (MQ=60)
                                                                                          ATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGTA     >  SRR4302540.6956782/1‑94 (MQ=60)
                                                                                          ATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGTA     <  SRR4302540.3211292/94‑1 (MQ=60)
                                                                                           TCTCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGTAC    <  SRR4302540.6608533/94‑1 (MQ=60)
                                                                                           TCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGTAC    <  SRR4302540.6292902/94‑1 (MQ=60)
                                                                                           TCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGTAC    <  SRR4302540.7630852/94‑1 (MQ=60)
                                                                                           TCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGTAC    <  SRR4302540.2242556/94‑1 (MQ=60)
                                                                                            CGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGTACG   >  SRR4302540.1650977/1‑94 (MQ=60)
                                                                                             GCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGTACGC  >  SRR4302540.5869971/1‑94 (MQ=60)
                                                                                             GCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGTACGC  >  SRR4302540.6435875/1‑94 (MQ=60)
                                                                                             |                                                                                             
TCCAGCCCCTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCACCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGTACGC  >  NC_000913/4437768‑4437954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑