Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,054 A→G H573R (CAC→CGC)  thrA → aspartokinase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,0540AG99.8% 1765.2 / ‑6.1 509H573R (CAC→CGC) thrAaspartokinase I
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (259/249);  total (259/250)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 531 total aligned reads displayed.

GAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATT  >  NC_000913/1963‑2144
                                                                                           |                                                                                          
  atatCATCTGCTGAACCCGGTCATTGTTGCCTGCGCTTCCAGCCAGGCAGTGGCGGATCAATATGACGACTTCCTGCGCGAAGGTTCCCACGtt                                                                                        >  1:8755191/1‑94 (MQ=255)
  atatCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGtt                                                                                        >  1:3621135/1‑94 (MQ=255)
  atatCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGtt                                                                                        <  2:7061003/94‑1 (MQ=255)
  atatCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGtt                                                                                        <  2:7400098/94‑1 (MQ=255)
   tatCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGACGGTTTCCGCGTTg                                                                                       >  1:3052961/1‑94 (MQ=255)
   tatCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTg                                                                                       >  1:8209879/1‑94 (MQ=255)
   tatCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTg                                                                                       <  1:4340963/94‑1 (MQ=255)
    atcatcTGCTGACCCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGt                                                                                      <  2:9180777/94‑1 (MQ=255)
     tcatcTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTc                                                                                     >  2:470653/1‑94 (MQ=255)
      catcTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCa                                                                                    <  1:3990013/94‑1 (MQ=255)
      catcTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCa                                                                                    <  2:1030050/94‑1 (MQ=255)
       atcTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCAc                                                                                   <  2:4321717/94‑1 (MQ=255)
       atcTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCAc                                                                                   >  2:1795418/1‑94 (MQ=255)
        tcTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACg                                                                                  <  2:4118484/94‑1 (MQ=255)
          tgctgAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGcc                                                                                <  2:8817864/94‑1 (MQ=255)
          tgctgAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGACCAATATGCCGACTTCCTGTGCGACGGTTTCCGCGTTGTCACGcg                                                                                >  1:8998073/1‑93 (MQ=255)
           gctgAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCg                                                                               <  1:7676730/94‑1 (MQ=255)
           gctgAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCg                                                                               >  2:6800765/1‑94 (MQ=255)
           gctgAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCg                                                                               >  2:5799149/1‑94 (MQ=255)
            ctgAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGa                                                                              >  2:3365828/1‑94 (MQ=255)
            ctgAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGa                                                                              >  1:9177123/1‑94 (MQ=255)
            ctgAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGa                                                                              >  1:5580487/1‑94 (MQ=255)
             tgAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGaa                                                                             >  2:5963086/1‑94 (MQ=255)
               aaCCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACa                                                                           >  2:9595554/1‑94 (MQ=255)
                aCCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACaa                                                                          <  1:1952499/94‑1 (MQ=255)
                  ccGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACaaaa                                                                        <  2:9259233/94‑1 (MQ=255)
                  ccGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACaaaa                                                                        <  1:593874/94‑1 (MQ=255)
                   cGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACaaaaa                                                                       <  1:1686132/94‑1 (MQ=255)
                      tCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGGTCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGc                                                                    >  1:2032700/1‑94 (MQ=255)
                       cATTGTTGACTGCACTTCCAGCTAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAAGcc                                                                   >  1:10402475/1‑94 (MQ=255)
                       cATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGcc                                                                   >  1:4404209/1‑94 (MQ=255)
                       cATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGcc                                                                   <  1:5800074/94‑1 (MQ=255)
                        aTTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCa                                                                  >  2:860487/1‑94 (MQ=255)
                        aTTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCa                                                                  <  1:4723124/94‑1 (MQ=255)
                          tgttgACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAac                                                                >  1:5002236/1‑94 (MQ=255)
                           gttgACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAaca                                                               <  2:3625522/94‑1 (MQ=255)
                           gttgACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAaca                                                               >  1:1453568/1‑94 (MQ=255)
                              gACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCt                                                            >  2:1447446/1‑94 (MQ=255)
                              gACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCt                                                            >  1:1241631/1‑94 (MQ=255)
                              gACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCt                                                            <  1:10552206/94‑1 (MQ=255)
                               aCTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCtc                                                           >  1:8490201/1‑94 (MQ=255)
                                cTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCtcg                                                          <  2:1172244/94‑1 (MQ=255)
                                cTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCtcg                                                          >  1:3257065/1‑94 (MQ=255)
                                  gCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCtcgtc                                                        <  1:7799903/94‑1 (MQ=255)
                                   cACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCtcgtcg                                                       >  1:2225837/1‑94 (MQ=255)
                                   cACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCtcgtcg                                                       <  1:2672705/94‑1 (MQ=255)
                                     cTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGAt                                                     <  1:4558532/94‑1 (MQ=255)
                                     cTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGAt                                                     <  1:1018282/94‑1 (MQ=255)
                                     cTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGAt                                                     <  1:6225867/94‑1 (MQ=255)
                                        ccagccagGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGa                                                  <  2:7908715/94‑1 (MQ=255)
                                         cagccagGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGAt                                                 >  1:6842527/1‑94 (MQ=255)
                                         cagccagGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGAt                                                 <  2:218454/94‑1 (MQ=255)
                                          cgccagGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGAtt                                                <  1:7157021/93‑1 (MQ=255)
                                          agccagGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGAtt                                                <  1:2454740/94‑1 (MQ=255)
                                             cagGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATtact                                             <  2:6635408/94‑1 (MQ=255)
                                              agGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATtacta                                            >  1:153041/1‑94 (MQ=255)
                                              agGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATtacta                                            >  1:5181448/1‑94 (MQ=255)
                                              agGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATtacta                                            >  2:7278658/1‑94 (MQ=255)
                                               ggCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATtactac                                           <  2:8250213/94‑1 (MQ=255)
                                                gCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTAcc                                          >  2:1989038/1‑94 (MQ=255)
                                                  aGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCAt                                        >  2:2443777/1‑94 (MQ=255)
                                                   gTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATc                                       >  2:6403634/1‑94 (MQ=255)
                                                    tGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCa                                      >  1:1183518/1‑94 (MQ=255)
                                                       gggATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGtt                                   <  2:6517475/93‑1 (MQ=255)
                                                       cGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGtt                                   <  2:1561658/94‑1 (MQ=255)
                                                       cGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGtt                                   <  1:6408574/94‑1 (MQ=255)
                                                        ggATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTg                                  >  2:10092875/1‑94 (MQ=255)
                                                         gATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTgg                                 >  1:4176324/1‑93 (MQ=255)
                                                         gATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGc                                 >  2:8025905/1‑94 (MQ=255)
                                                         gATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGc                                 <  2:2809761/94‑1 (MQ=255)
                                                          aTCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCg                                >  1:7482370/1‑94 (MQ=255)
                                                          aTCAATATGCCGACTCCCTGCGCGAAGGTGTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCg                                <  1:6039728/94‑1 (MQ=255)
                                                            cAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGtt                              >  1:2837280/1‑94 (MQ=255)
                                                            cAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGtt                              >  1:3589519/1‑94 (MQ=255)
                                                             aaTATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTa                             >  2:7614910/1‑94 (MQ=255)
                                                             aaTATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTa                             >  1:10003949/1‑94 (MQ=255)
                                                              atatGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTAt                            >  2:4904031/1‑94 (MQ=255)
                                                              atatGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTAt                            <  1:9814570/94‑1 (MQ=255)
                                                                atGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATgc                          >  2:319764/1‑94 (MQ=255)
                                                                atGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATgc                          >  2:5168835/1‑94 (MQ=255)
                                                                atGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATgc                          >  2:2307422/1‑94 (MQ=255)
                                                                atGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATgc                          >  2:8381677/1‑94 (MQ=255)
                                                                 tGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATgcg                         <  2:6255082/94‑1 (MQ=255)
                                                                  gCCTACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATgcgg                        <  1:96409/94‑1 (MQ=255)
                                                                  gCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATgcgg                        <  2:5275155/94‑1 (MQ=255)
                                                                     gACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGcggcgg                     >  1:5471389/1‑94 (MQ=255)
                                                                      aCTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGa                    <  2:5444515/94‑1 (MQ=255)
                                                                      aCTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGa                    <  2:3950877/94‑1 (MQ=255)
                                                                       cTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACCCCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGaa                   <  1:9251298/94‑1 (MQ=255)
                                                                         tCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGaaaa                 <  2:294722/94‑1 (MQ=255)
                                                                           cTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAAt               <  2:10567175/94‑1 (MQ=255)
                                                                             gcgcGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATcg             <  2:1884739/94‑1 (MQ=255)
                                                                             gcgcGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATcg             <  1:10022699/94‑1 (MQ=255)
                                                                              cgcgAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATcgc            <  2:25936/94‑1 (MQ=255)
                                                                               gcgAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATcgcg           <  1:2343293/94‑1 (MQ=255)
                                                                                 gAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCgcggc         >  1:8018758/1‑94 (MQ=255)
                                                                                 gAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCgcggc         >  1:3374790/1‑94 (MQ=255)
                                                                                   aGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGt       >  1:9498118/1‑94 (MQ=255)
                                                                                       ttCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAAt   <  2:5673929/94‑1 (MQ=255)
                                                                                        tCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGt                               <  2:6944569/65‑1 (MQ=255)
                                                                                           |                                                                                          
GAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATT  >  NC_000913/1963‑2144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 294 total aligned reads displayed.
AAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCC  >  NC_000913/1961‑2146
                                                                                             |                                                                                            
AAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCG                                                                                              <  SRR4302545.6436323/94‑1 (MQ=60)
AAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCG                                                                                              >  SRR4302545.8917735/1‑94 (MQ=60)
 AGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGC                                                                                             >  SRR4302545.4416386/1‑94 (MQ=60)
 AGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGC                                                                                             >  SRR4302545.4496542/1‑94 (MQ=60)
 AGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGC                                                                                             <  SRR4302545.2536662/94‑1 (MQ=60)
 AGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGC                                                                                             >  SRR4302545.10801467/1‑94 (MQ=60)
   AATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGT                                                                                           >  SRR4302545.6131718/1‑94 (MQ=60)
   AATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGT                                                                                           >  SRR4302545.6131719/1‑94 (MQ=60)
    ATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTT                                                                                          >  SRR4302545.3709434/1‑94 (MQ=60)
    ATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTT                                                                                          <  SRR4302545.5950156/94‑1 (MQ=60)
    ATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTT                                                                                          <  SRR4302545.6133144/94‑1 (MQ=60)
    ATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTT                                                                                          <  SRR4302545.7160224/94‑1 (MQ=60)
    ATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTT                                                                                          <  SRR4302545.1548165/94‑1 (MQ=60)
    ATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTT                                                                                          >  SRR4302545.9265318/1‑94 (MQ=60)
     TATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGACGGTTTCCGCGTTG                                                                                         >  SRR4302545.3127818/1‑94 (MQ=60)
     TATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTG                                                                                         <  SRR4302545.4450523/94‑1 (MQ=60)
     TATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTG                                                                                         >  SRR4302545.10374083/1‑94 (MQ=60)
      ATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGT                                                                                        >  SRR4302545.8270695/1‑94 (MQ=60)
       TCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTC                                                                                       <  SRR4302545.9861812/94‑1 (MQ=60)
       TCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTC                                                                                       >  SRR4302545.2380697/1‑94 (MQ=60)
        CATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCA                                                                                      >  SRR4302545.6025904/1‑94 (MQ=60)
        CATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCA                                                                                      <  SRR4302545.4089810/94‑1 (MQ=60)
        CATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCA                                                                                      <  SRR4302545.5714604/94‑1 (MQ=60)
        CATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCA                                                                                      >  SRR4302545.6025903/1‑94 (MQ=60)
        CATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCA                                                                                      <  SRR4302545.9208376/94‑1 (MQ=60)
        CACCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCA                                                                                      <  SRR4302545.5571570/94‑1 (MQ=60)
         ATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCAC                                                                                     <  SRR4302545.7882875/94‑1 (MQ=60)
         ATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCAC                                                                                     >  SRR4302545.942460/1‑94 (MQ=60)
         ATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCAC                                                                                     <  SRR4302545.4269263/94‑1 (MQ=60)
         ATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCAC                                                                                     >  SRR4302545.3518800/1‑94 (MQ=60)
          TCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACG                                                                                    <  SRR4302545.2021466/94‑1 (MQ=60)
            TGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCC                                                                                  >  SRR4302545.9260061/1‑94 (MQ=60)
            TGCTGAACCCGGTCATTGTTGACTGCACTCCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCC                                                                                  <  SRR4302545.10230994/94‑1 (MQ=60)
             GCTGACCCCGCACATTATTGACTGCACTTCCAGCCAAGCCGTGGCGGATCAATATGCCGGCTTCCTGAGCGAAGGTTTCCGCGTTGTaacgaca                                                                                 <  SRR4302545.9646079/94‑8 (MQ=47)
             GCGGACCCCGGTCATTGTTGATTGCACTTCCAGCGAGGCAGTGG‑GGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGA                                                                                <  SRR4302545.8654809/94‑1 (MQ=60)
              CTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGA                                                                                >  SRR4302545.4758050/1‑94 (MQ=60)
              CTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGA                                                                                >  SRR4302545.9446306/1‑94 (MQ=60)
              CTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGA                                                                                >  SRR4302545.350164/1‑94 (MQ=60)
               TGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAA                                                                               >  SRR4302545.4012633/1‑94 (MQ=60)
               TGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAA                                                                               >  SRR4302545.10316437/1‑94 (MQ=60)
                GAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAAC                                                                              <  SRR4302545.8330765/94‑1 (MQ=60)
                GAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAAC                                                                              <  SRR4302545.8639929/94‑1 (MQ=60)
                 AACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACA                                                                             >  SRR4302545.2096847/1‑94 (MQ=60)
                  ACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAA                                                                            <  SRR4302545.2000912/94‑1 (MQ=60)
                  ACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAA                                                                            >  SRR4302545.3206861/1‑94 (MQ=60)
                  ACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAA                                                                            >  SRR4302545.5772726/1‑94 (MQ=60)
                   CCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAA                                                                           >  SRR4302545.6740295/1‑94 (MQ=60)
                   CCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAA                                                                           <  SRR4302545.10100315/94‑1 (MQ=60)
                    CCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAA                                                                          <  SRR4302545.607272/94‑1 (MQ=60)
                     CGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAA                                                                         <  SRR4302545.1726134/94‑1 (MQ=60)
                     CGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAA                                                                         <  SRR4302545.9035039/94‑1 (MQ=60)
                     CGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAA                                                                         <  SRR4302545.1825724/94‑1 (MQ=60)
                     CGGGCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGGTGTCACGCCGAACAAAAA                                                                         >  SRR4302545.1214827/1‑94 (MQ=60)
                       GTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGG                                                                       <  SRR4302545.3927106/94‑1 (MQ=60)
                        TCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGGTCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGC                                                                      >  SRR4302545.2082846/1‑94 (MQ=60)
                         CATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCC                                                                     <  SRR4302545.5956088/94‑1 (MQ=60)
                         CATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCC                                                                     >  SRR4302545.4515971/1‑94 (MQ=60)
                         CATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTACGGGTTGTAACACCGAACAAAAAGGCA                                                                     >  SRR4302545.3163901/1‑94 (MQ=60)
                          ATTGTTGACTGCTCTTCCAGCCAGGCAGTGGCGTATCAATATGCCGGCTTCGTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACCAAAAGGCCA                                                                    <  SRR4302545.7963823/94‑1 (MQ=60)
                          ATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCA                                                                    <  SRR4302545.4843558/94‑1 (MQ=60)
                          ATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCA                                                                    >  SRR4302545.4366447/1‑94 (MQ=60)
                            TGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAAC                                                                  >  SRR4302545.5128954/1‑94 (MQ=60)
                            TGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAAC                                                                  >  SRR4302545.10312895/1‑94 (MQ=60)
                            TGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAAC                                                                  >  SRR4302545.2430316/1‑94 (MQ=60)
                             GTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACA                                                                 >  SRR4302545.1486795/1‑94 (MQ=60)
                             GTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACA                                                                 <  SRR4302545.1452204/94‑1 (MQ=60)
                             GTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACA                                                                 >  SRR4302545.1486796/1‑94 (MQ=60)
                             GTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACA                                                                 >  SRR4302545.4180302/1‑94 (MQ=60)
                             GTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACA                                                                 <  SRR4302545.926872/94‑1 (MQ=60)
                              TTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACAC                                                                <  SRR4302545.1179760/94‑1 (MQ=60)
                              TTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACAC                                                                <  SRR4302545.1072217/94‑1 (MQ=60)
                              TTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACAC                                                                <  SRR4302545.4891500/94‑1 (MQ=60)
                               TGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACC                                                               <  SRR4302545.8063404/94‑1 (MQ=60)
                               TGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACC                                                               <  SRR4302545.423153/94‑1 (MQ=60)
                               TGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACC                                                               >  SRR4302545.8011194/1‑94 (MQ=60)
                               TGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACC                                                               >  SRR4302545.1018294/1‑94 (MQ=60)
                               TGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACC                                                               <  SRR4302545.7740624/94‑1 (MQ=60)
                               TGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACC                                                               >  SRR4302545.953027/1‑94 (MQ=60)
                                GACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCT                                                              >  SRR4302545.1269588/1‑94 (MQ=60)
                                GACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCT                                                              <  SRR4302545.10863019/94‑1 (MQ=60)
                                GACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATACCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAACAAGGCCAACACCT                                                              <  SRR4302545.9664191/94‑1 (MQ=60)
                                  CTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCG                                                            <  SRR4302545.10012185/94‑1 (MQ=60)
                                  CTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCG                                                            >  SRR4302545.3336999/1‑94 (MQ=60)
                                   TGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGT                                                           <  SRR4302545.3691769/94‑1 (MQ=60)
                                   TGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGT                                                           >  SRR4302545.1642967/1‑94 (MQ=60)
                                   TGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGT                                                           >  SRR4302545.1258823/1‑94 (MQ=60)
                                    GCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTC                                                          <  SRR4302545.8020733/94‑1 (MQ=60)
                                    GCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTC                                                          <  SRR4302545.8098492/94‑1 (MQ=60)
                                     CACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCG                                                         >  SRR4302545.2280146/1‑94 (MQ=60)
                                     CACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCG                                                         <  SRR4302545.2737101/94‑1 (MQ=60)
                                     CACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCG                                                         >  SRR4302545.4828220/1‑94 (MQ=60)
                                      CCTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGA                                                        <  SRR4302545.8794551/94‑1 (MQ=60)
                                       CTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGAT                                                       <  SRR4302545.5529655/94‑1 (MQ=60)
                                       CTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGAT                                                       <  SRR4302545.6395488/94‑1 (MQ=60)
                                       CTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGAT                                                       <  SRR4302545.1040719/94‑1 (MQ=60)
                                        TTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATG                                                      <  SRR4302545.4613995/94‑1 (MQ=60)
                                        TTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATG                                                      <  SRR4302545.1001098/94‑1 (MQ=60)
                                         TCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGG                                                     <  SRR4302545.1623754/94‑1 (MQ=60)
                                         TCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGG                                                     <  SRR4302545.1317468/94‑1 (MQ=60)
                                         TCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGG                                                     <  SRR4302545.4092345/94‑1 (MQ=60)
                                          CCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGA                                                    >  SRR4302545.2436226/1‑94 (MQ=60)
                                          CCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGA                                                    >  SRR4302545.2610168/1‑94 (MQ=60)
                                           CAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGAT                                                   >  SRR4302545.9593198/1‑94 (MQ=60)
                                           CAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGAT                                                   >  SRR4302545.7030475/1‑94 (MQ=60)
                                           CAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGAT                                                   <  SRR4302545.4710901/94‑1 (MQ=60)
                                           CAGCCAGGCAGTGGCGGATCAATATGCCGAATTCCTGCGCGAAGGTTTCCGCGTTGTCAAGACGAACAAAAAGGCCAACACCTCGTCGATGGAT                                                   >  SRR4302545.1925638/1‑94 (MQ=60)
                                            CGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATT                                                  <  SRR4302545.7355716/94‑1 (MQ=60)
                                            AGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGACGGTTTCCGCGTTGTCACGCCGAACAAAAATGCCAACACCTCGTCGATGGATT                                                  >  SRR4302545.5172701/1‑94 (MQ=60)
                                            AGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATT                                                  <  SRR4302545.2514196/94‑1 (MQ=60)
                                            AGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATT                                                  <  SRR4302545.7272122/94‑1 (MQ=60)
                                             GCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTA                                                 <  SRR4302545.3339901/94‑1 (MQ=60)
                                             GCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTA                                                 <  SRR4302545.4528356/94‑1 (MQ=60)
                                              CCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTAC                                                <  SRR4302545.143157/94‑1 (MQ=60)
                                              CCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTAC                                                >  SRR4302545.239218/1‑94 (MQ=60)
                                              CCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTAC                                                >  SRR4302545.1706255/1‑94 (MQ=60)
                                                AGGCAGTGGCGGATCAATATGCCTACTTCCTGCGCGAAGGTTGCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTA                                              <  SRR4302545.7562266/94‑1 (MQ=60)
                                                AGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTA                                              >  SRR4302545.6591777/1‑94 (MQ=60)
                                                AGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTA                                              >  SRR4302545.5314842/1‑94 (MQ=60)
                                                AGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTA                                              >  SRR4302545.4456762/1‑94 (MQ=60)
                                                 GGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTAC                                             <  SRR4302545.6454151/94‑1 (MQ=60)
                                                 GGCAGTGGCGGATCAATATGCAGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTAC                                             <  SRR4302545.2370285/94‑1 (MQ=60)
                                                  GCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACC                                            >  SRR4302545.6224916/1‑94 (MQ=60)
                                                    AGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCAT                                          <  SRR4302545.3509478/94‑1 (MQ=60)
                                                    AGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCAT                                          >  SRR4302545.3994409/1‑94 (MQ=60)
                                                     GTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATC                                         >  SRR4302545.1071522/1‑94 (MQ=60)
                                                        GCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGT                                      >  SRR4302545.10707084/1‑94 (MQ=60)
                                                         CGGATCAATATGCCGACTTCCTGCGCGAAGTTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTT                                     <  SRR4302545.5715776/94‑1 (MQ=60)
                                                         CGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGTATTACTACCATCAGTT                                     >  SRR4302545.9827740/1‑94 (MQ=60)
                                                         CGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTT                                     >  SRR4302545.3431693/1‑94 (MQ=60)
                                                         CGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTT                                     <  SRR4302545.3033831/94‑1 (MQ=60)
                                                         CGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTG                                     >  SRR4302545.7984862/1‑94 (MQ=60)
                                                         CGGATCAATATGCCGACTTCCTGCGCGAAAGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTT                                     <  SRR4302545.9014218/94‑1 (MQ=60)
                                                          GGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTG                                    >  SRR4302545.155177/1‑94 (MQ=60)
                                                          GGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTG                                    >  SRR4302545.4582862/1‑94 (MQ=60)
                                                           GATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGG                                   >  SRR4302545.4280571/1‑94 (MQ=60)
                                                           GATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGC                                   >  SRR4302545.5648312/1‑94 (MQ=60)
                                                           GATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGC                                   >  SRR4302545.10660965/1‑94 (MQ=60)
                                                           GATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGC                                   >  SRR4302545.6227248/1‑94 (MQ=60)
                                                            ATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCG                                  >  SRR4302545.7694138/1‑94 (MQ=60)
                                                            ATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCG                                  <  SRR4302545.3502622/94‑1 (MQ=60)
                                                            ATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCG                                  >  SRR4302545.5826421/1‑94 (MQ=60)
                                                             TCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGT                                 <  SRR4302545.942027/94‑1 (MQ=60)
                                                              CAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTT                                <  SRR4302545.2025904/94‑1 (MQ=60)
                                                              CAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTT                                >  SRR4302545.3677116/1‑94 (MQ=60)
                                                              CAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTT                                <  SRR4302545.2640318/94‑1 (MQ=60)
                                                               AATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTA                               <  SRR4302545.1177465/94‑1 (MQ=60)
                                                               AATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTA                               >  SRR4302545.2998030/1‑94 (MQ=60)
                                                               AATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTA                               >  SRR4302545.10300538/1‑94 (MQ=60)
                                                               AATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTA                               >  SRR4302545.10300537/1‑94 (MQ=60)
                                                               AATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAACGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTA                               >  SRR4302545.4653240/1‑94 (MQ=60)
                                                                ATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTAT                              >  SRR4302545.8901367/1‑94 (MQ=60)
                                                                ATATGCCGACTTCCTGCGAGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAGCACCTCGTCGATGGATTACTACCATCAGTTGCGTTAT                              <  SRR4302545.1785923/94‑1 (MQ=60)
                                                                 TATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATG                             <  SRR4302545.76025/94‑1 (MQ=60)
                                                                   TGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCG                           <  SRR4302545.2346632/94‑1 (MQ=60)
                                                                    GCCTACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGG                          <  SRR4302545.98637/94‑1 (MQ=60)
                                                                    GCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAGCACCTCGTCGATGAATTACTCCCATCAatttccatctccgg                          >  SRR4302545.8442085/1‑80 (MQ=60)
                                                                    GCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGTGTTATGCGG                          <  SRR4302545.366817/94‑1 (MQ=60)
                                                                     CCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGC                         >  SRR4302545.10548722/1‑94 (MQ=60)
                                                                      CGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCACCACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCG                        >  SRR4302545.7944149/1‑94 (MQ=60)
                                                                      CGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCG                        >  SRR4302545.3112236/1‑94 (MQ=60)
                                                                      CGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCG                        >  SRR4302545.3047604/1‑94 (MQ=60)
                                                                       GACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGG                       >  SRR4302545.7573783/1‑94 (MQ=60)
                                                                       GACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGG                       >  SRR4302545.2807790/1‑94 (MQ=60)
                                                                       GACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGG                       >  SRR4302545.5615003/1‑94 (MQ=60)
                                                                       GACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGG                       <  SRR4302545.6540678/94‑1 (MQ=60)
                                                                        ACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTAGTACCATCAGTTGCGTTATGCGGGGGA                      >  SRR4302545.5450552/1‑94 (MQ=60)
                                                                        ACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCAGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGA                      >  SRR4302545.1091267/1‑94 (MQ=60)
                                                                          TTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAA                    <  SRR4302545.5075798/94‑1 (MQ=60)
                                                                             CTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAAT                 <  SRR4302545.2222608/94‑1 (MQ=60)
                                                                             CTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAAT                 >  SRR4302545.10669090/1‑94 (MQ=60)
                                                                             CTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAAT                 <  SRR4302545.8609656/94‑1 (MQ=60)
                                                                              TGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATC                <  SRR4302545.7456662/94‑1 (MQ=60)
                                                                              TGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATC                <  SRR4302545.8503628/94‑1 (MQ=60)
                                                                              TGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATC                <  SRR4302545.5173428/94‑1 (MQ=60)
                                                                              TGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATC                <  SRR4302545.5234292/94‑1 (MQ=60)
                                                                               GCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCG               <  SRR4302545.1267680/94‑1 (MQ=60)
                                                                               GCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCG               <  SRR4302545.10319728/94‑1 (MQ=60)
                                                                               GCGCGAAGGTTTCCGCGTTCTCACGCCGAACAAAAAGGCCAACGCCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCG               <  SRR4302545.4495766/94‑1 (MQ=60)
                                                                                CGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGC              >  SRR4302545.6044709/1‑94 (MQ=60)
                                                                                CGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGC              >  SRR4302545.6582730/1‑94 (MQ=60)
                                                                                 GCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCG             >  SRR4302545.8922570/1‑94 (MQ=60)
                                                                                 GCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCG             <  SRR4302545.3441880/94‑1 (MQ=60)
                                                                                 GCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCG             >  SRR4302545.6968223/1‑94 (MQ=60)
                                                                                 GCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCG             >  SRR4302545.2872585/1‑94 (MQ=60)
                                                                                  CGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGG            >  SRR4302545.10806195/1‑94 (MQ=60)
                                                                                  CGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGG            >  SRR4302545.6382238/1‑94 (MQ=60)
                                                                                  CGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGG            >  SRR4302545.9153705/1‑94 (MQ=60)
                                                                                  CGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGG            <  SRR4302545.9454403/94‑1 (MQ=60)
                                                                                   GAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGC           >  SRR4302545.10366615/1‑94 (MQ=60)
                                                                                   GAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGC           >  SRR4302545.9820946/1‑94 (MQ=60)
                                                                                   GAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGC           >  SRR4302545.8247088/1‑94 (MQ=60)
                                                                                    AAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCG          <  SRR4302545.2201413/94‑1 (MQ=60)
                                                                                     AGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAACATCGCGGCGT         >  SRR4302545.9777695/1‑94 (MQ=60)
                                                                                     AGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGT         >  SRR4302545.9777696/1‑94 (MQ=60)
                                                                                      GGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTA        <  SRR4302545.2881488/94‑1 (MQ=60)
                                                                                       GTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAA       >  SRR4302545.7716784/1‑94 (MQ=60)
                                                                                         TTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAAT     >  SRR4302545.8367062/1‑94 (MQ=60)
                                                                                           CCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTC   <  SRR4302545.5011053/94‑1 (MQ=60)
                                                                                            CGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCC  <  SRR4302545.7922257/94‑1 (MQ=60)
                                                                                            CGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCC  <  SRR4302545.10326257/94‑1 (MQ=60)
                                                                                             |                                                                                            
AAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCC  >  NC_000913/1961‑2146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑