Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,054	A→G	H573R (CAC→CGC)	thrA →	aspartokinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,054	0	A	G	100.0%	972.9 / NA	280	H573R (CAC→CGC)	thrA	aspartokinase I
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (147/133); total (147/133)

Only 100 of 294 total aligned reads displayed.

ATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAAT  >  NC_000913/1965‑2143

atatCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGt                                                                                        <  2:4578656/93‑1 (MQ=255)

atatCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGt                                                                                        <  1:5994748/93‑1 (MQ=255)

atatCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGt                                                                                        >  2:4875278/1‑93 (MQ=255)

 tatCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGtt                                                                                       <  1:5650822/93‑1 (MQ=255)

 tatCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGACAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGtt                                                                                       >  1:2617562/1‑93 (MQ=255)

  atcatcTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTg                                                                                      >  2:1300001/1‑93 (MQ=255)

    catcTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGAATTCCTGCGCGAAGGTTTCCGCGTTGTc                                                                                    >  2:5584084/1‑93 (MQ=255)

      tcTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCAc                                                                                  <  1:1089413/93‑1 (MQ=255)

        tgctgAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGc                                                                                <  1:1669641/93‑1 (MQ=255)

         gctgATCCCGGTCATTGTTGACTGCACATCCAGACAGGCAGTGGCGAATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGcc                                                                               <  2:2391070/93‑1 (MQ=255)

           tgATCCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGa                                                                             <  1:1920404/93‑1 (MQ=255)

            gAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGaa                                                                            >  1:3683098/1‑93 (MQ=255)

             aaCCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAAc                                                                           >  2:3683368/1‑93 (MQ=255)

             aaCCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAAc                                                                           >  2:439573/1‑93 (MQ=255)

             aaCCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAAc                                                                           >  1:5356887/1‑93 (MQ=255)

              aCCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACa                                                                          <  2:1608387/93‑1 (MQ=255)

              aCCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACa                                                                          >  2:547863/1‑93 (MQ=255)

              aCCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACa                                                                          >  1:2851113/1‑93 (MQ=255)

                ccGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACaaa                                                                        >  2:4769236/1‑93 (MQ=255)

                ccGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACaaa                                                                        >  1:2536856/1‑93 (MQ=255)

                 cGGTCATTGTTGACTGCACTTCCCGCCAGGCAGTGGCGGATCAATATGCCGACTTCATGAGCGGAGGCTTCCGCGGTGTCACGCCGAACaaca                                                                       >  2:2901437/1‑91 (MQ=255)

                 cGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACaaaa                                                                       >  1:1375371/1‑93 (MQ=255)

                  ggTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACaaaaa                                                                      <  2:2976621/93‑1 (MQ=255)

                  ggTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACaaaaa                                                                      <  1:3559010/93‑1 (MQ=255)

                  ggTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACaaaaa                                                                      >  2:1846686/1‑93 (MQ=255)

                     cATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGc                                                                   <  2:4837369/93‑1 (MQ=255)

                     cATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGc                                                                   <  2:3477474/93‑1 (MQ=255)

                      aTTGTTGACTGTACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGGGCGAAGGTTTCCGCGTTGTCACGCCGACAAAAAAGGca                                                                  >  1:1608315/1‑92 (MQ=255)

                      aTTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTACCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGcc                                                                  <  1:6473500/93‑1 (MQ=255)

                       ttgttgACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCa                                                                 <  2:2652719/93‑1 (MQ=255)

                       ttgttgACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCa                                                                 >  2:237510/1‑93 (MQ=255)

                       ttgttgACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACCCCGAACAAAAAGGCCa                                                                 >  2:3798572/1‑93 (MQ=255)

                         gttgACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAac                                                               <  2:5106801/93‑1 (MQ=255)

                            gACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACAcc                                                            <  1:6061072/93‑1 (MQ=255)

                             aCTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAGGGCCAACACCt                                                           <  2:499176/93‑1 (MQ=255)

                              cTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCtc                                                          >  1:4213502/1‑93 (MQ=255)

                                 cACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCtcgtc                                                       <  2:3144179/93‑1 (MQ=255)

                                  aCTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCtcgtcg                                                      >  2:1442097/1‑93 (MQ=255)

                                  aCTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCtcgtcg                                                      >  2:2558561/1‑93 (MQ=255)

                                   cTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGa                                                     <  1:4023581/93‑1 (MQ=255)

                                   cTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGa                                                     >  1:4478110/1‑93 (MQ=255)

                                     tCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATg                                                   <  1:2767031/93‑1 (MQ=255)

                                      ccagccagGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATgg                                                  <  2:1385416/93‑1 (MQ=255)

                                      ccagccagGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATgg                                                  >  1:5152688/1‑93 (MQ=255)

                                       cagccagGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGa                                                 <  2:5783500/93‑1 (MQ=255)

                                        agccagGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGAt                                                <  2:2582257/93‑1 (MQ=255)

                                        agccagGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGAt                                                <  2:3078321/93‑1 (MQ=255)

                                        agccagGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGAt                                                <  1:2035683/93‑1 (MQ=255)

                                           cagGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGAtttc                                             <  2:1956436/93‑3 (MQ=255)

                                           cagGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATtac                                             >  2:5247045/1‑93 (MQ=255)

                                              gCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATtactac                                          >  1:6268631/1‑93 (MQ=255)

                                               cAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTAcc                                         >  1:962197/1‑93 (MQ=255)

                                                aGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCa                                        <  2:2231286/93‑1 (MQ=255)

                                                 gTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCAt                                       >  1:2134651/1‑93 (MQ=255)

                                                 gTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCAt                                       >  2:3359650/1‑93 (MQ=255)

                                                 gTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCAt                                       >  2:4720467/1‑93 (MQ=255)

                                                 gTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCAt                                       >  2:281488/1‑93 (MQ=255)

                                                   ggCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCa                                     <  2:1722014/93‑1 (MQ=255)

                                                   ggCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCa                                     <  2:2136993/93‑1 (MQ=255)

                                                    gCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAg                                    <  1:1752482/93‑1 (MQ=255)

                                                     cGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGt                                   <  1:6410896/93‑1 (MQ=255)

                                                       gATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTg                                 >  2:2503101/1‑93 (MQ=255)

                                                        aTCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGc                                <  1:5295986/93‑1 (MQ=255)

                                                          cAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGt                              >  2:3449294/1‑93 (MQ=255)

                                                           aaTATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGtt                             >  2:4227588/1‑93 (MQ=255)

                                                           aaTATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGtt                             <  1:3007997/93‑1 (MQ=255)

                                                            atatGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTa                            >  1:4346187/1‑93 (MQ=255)

                                                            atatGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTa                            >  2:6053524/1‑93 (MQ=255)

                                                              atGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAATAGGGCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATg                          >  1:2667044/1‑93 (MQ=255)

                                                               tGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCGCGTCGATGGATTACTACCATCAGTTGCGTTATgc                         <  1:3936949/93‑1 (MQ=255)

                                                                gCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATgcg                        >  1:4572997/1‑93 (MQ=255)

                                                                gCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATgcg                        >  1:223953/1‑93 (MQ=255)

                                                                gCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATgcg                        >  2:672180/1‑93 (MQ=255)

                                                                gCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATgcg                        <  1:4871044/93‑1 (MQ=255)

                                                                  cGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATgcggc                      <  2:921847/93‑1 (MQ=255)

                                                                  cGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATgcggc                      >  1:3510420/1‑93 (MQ=255)

                                                                  cGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATgcggc                      >  1:5791758/1‑93 (MQ=255)

                                                                   gACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATgcggcg                     <  2:3200240/93‑1 (MQ=255)

                                                                   gACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATgcggcg                     >  2:2051314/1‑93 (MQ=255)

                                                                   gACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATgcggcg                     >  2:454283/1‑93 (MQ=255)

                                                                     cTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGa                   <  1:4734203/93‑1 (MQ=255)

                                                                      ttCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGaa                  >  2:1110465/1‑93 (MQ=255)

                                                                      ttCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGaa                  >  2:3629692/1‑93 (MQ=255)

                                                                       tCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGaaa                 >  1:5852893/1‑93 (MQ=255)

                                                                         cTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGaaaaa               >  1:5589384/1‑93 (MQ=255)

                                                                         cTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGaaaaa               >  2:807111/1‑93 (MQ=255)

                                                                         cTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGaaaaa               <  1:3205347/93‑1 (MQ=255)

                                                                          tGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAAt              >  2:1577651/1‑93 (MQ=255)

                                                                          tGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAAt              >  1:1239686/1‑93 (MQ=255)

                                                                          tGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAAt              >  2:2712591/1‑93 (MQ=255)

                                                                            cgcgAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATcg            >  1:4068377/1‑93 (MQ=255)

                                                                            cgcgAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATcg            >  1:108407/1‑93 (MQ=255)

                                                                             gcgAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATcgc           >  2:5904060/1‑93 (MQ=255)

                                                                              cgAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATcgcg          <  2:594893/93‑1 (MQ=255)

                                                                              cgAAGCTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATcgcg          <  1:2395425/93‑1 (MQ=255)

                                                                               gAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCgcgg         >  2:1763446/1‑93 (MQ=255)

                                                                                aaGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCgcggc        >  2:4496018/1‑93 (MQ=255)

                                                                                 aGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCgcggcg       >  1:792341/1‑93 (MQ=255)

                                                                                    tttCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTaa    <  1:5013900/93‑1 (MQ=255)

                                                                                    tttCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTaa    <  2:5361477/93‑1 (MQ=255)

ATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAAT  >  NC_000913/1965‑2143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCT  >  NC_000913/1961‑2147

AAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCG                                                                                               <  SRR4302537.4566968/94‑1 (MQ=60)

AAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCG                                                                                               <  SRR4302537.1252330/94‑1 (MQ=60)

AAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGAAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCG                                                                                               >  SRR4302537.6389693/1‑94 (MQ=60)

  GAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCG                                                                                             <  SRR4302537.6222848/94‑1 (MQ=60)

  AAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCG                                                                                             <  SRR4302537.118085/94‑1 (MQ=60)

   AATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGT                                                                                            >  SRR4302537.6056477/1‑94 (MQ=60)

    ATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTT                                                                                           <  SRR4302537.6035918/94‑1 (MQ=60)

    ATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTT                                                                                           >  SRR4302537.436727/1‑94 (MQ=60)

    ATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGACAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTT                                                                                           >  SRR4302537.2635864/1‑94 (MQ=60)

     TATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTG                                                                                          <  SRR4302537.5689494/94‑1 (MQ=60)

      ATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGT                                                                                         >  SRR4302537.4022632/1‑94 (MQ=60)

        CATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCA                                                                                       >  SRR4302537.801886/1‑94 (MQ=60)

        CATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCA                                                                                       <  SRR4302537.217096/94‑1 (MQ=60)

        CATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCA                                                                                       >  SRR4302537.2242377/1‑94 (MQ=60)

        CATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCA                                                                                       <  SRR4302537.1721841/94‑1 (MQ=60)

         ATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCAC                                                                                      <  SRR4302537.3904784/94‑1 (MQ=60)

         ATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCAC                                                                                      <  SRR4302537.3626052/94‑1 (MQ=60)

         ATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGCATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCAC                                                                                      <  SRR4302537.3490889/94‑1 (MQ=60)

          TCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACG                                                                                     <  SRR4302537.1096939/94‑1 (MQ=60)

          TCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACG                                                                                     >  SRR4302537.4691723/1‑94 (MQ=60)

           CTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGC                                                                                    >  SRR4302537.5508709/1‑94 (MQ=60)

            TGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCC                                                                                   <  SRR4302537.1681336/94‑1 (MQ=60)

            CGCTGACCCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCC                                                                                   <  SRR4302537.2012429/94‑1 (MQ=60)

             GCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCG                                                                                  <  SRR4302537.5754007/94‑1 (MQ=60)

             GCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGAGAAGGTTTCCGCGTTGTCACGCCG                                                                                  >  SRR4302537.4004139/1‑94 (MQ=60)

               TGATCCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAA                                                                                <  SRR4302537.1933742/94‑1 (MQ=60)

               TGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAA                                                                                >  SRR4302537.3708999/1‑94 (MQ=60)

               TGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAA                                                                                >  SRR4302537.3721064/1‑94 (MQ=60)

                TAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAAC                                                                               <  SRR4302537.1231377/94‑1 (MQ=60)

                GAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAAC                                                                               >  SRR4302537.2832894/1‑94 (MQ=60)

                GAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAAC                                                                               >  SRR4302537.5393575/1‑94 (MQ=60)

                GAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAAC                                                                               >  SRR4302537.3558955/1‑94 (MQ=60)

                 AACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACA                                                                              <  SRR4302537.2528008/94‑1 (MQ=60)

                 AACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACA                                                                              >  SRR4302537.5990600/1‑94 (MQ=60)

                 AACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACA                                                                              >  SRR4302537.2871084/1‑94 (MQ=60)

                 AACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACA                                                                              >  SRR4302537.873738/1‑94 (MQ=60)

                   CCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAA                                                                            >  SRR4302537.2554657/1‑94 (MQ=60)

                    CCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAA                                                                           >  SRR4302537.1384972/1‑94 (MQ=60)

                     CGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAA                                                                          <  SRR4302537.4419965/94‑1 (MQ=60)

                      GTTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACACCGAACAAAAAG                                                                         <  SRR4302537.778111/94‑1 (MQ=60)

                      GGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAG                                                                         <  SRR4302537.2105296/94‑1 (MQ=60)

                      GGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAG                                                                         <  SRR4302537.2898017/94‑1 (MQ=60)

                      GGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAG                                                                         <  SRR4302537.1347592/94‑1 (MQ=60)

                      GGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAG                                                                         <  SRR4302537.3584120/94‑1 (MQ=60)

                        TCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGC                                                                       <  SRR4302537.2308577/94‑1 (MQ=60)

                         CATTGTTGACTGTACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGGGCGAAGGTTTCCGCGTTGTCACGCCGACAAAAAAGGCA                                                                      >  SRR4302537.1619560/1‑94 (MQ=60)

                          ATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTACCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCA                                                                     <  SRR4302537.6517426/94‑1 (MQ=60)

                           TTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAA                                                                    <  SRR4302537.5250197/94‑1 (MQ=60)

                           TTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAA                                                                    >  SRR4302537.3555607/1‑94 (MQ=60)

                            TGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAAC                                                                   >  SRR4302537.4315142/1‑94 (MQ=60)

                            TGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAAC                                                                   >  SRR4302537.1819686/1‑94 (MQ=60)

                             GTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACA                                                                  <  SRR4302537.6039995/94‑1 (MQ=60)

                               TGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACC                                                                <  SRR4302537.6141711/94‑1 (MQ=60)

                                GACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCT                                                               >  SRR4302537.583157/1‑94 (MQ=60)

                                GACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCT                                                               <  SRR4302537.6102717/94‑1 (MQ=60)

                                GACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCT                                                               <  SRR4302537.130661/94‑1 (MQ=60)

                                GACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCT                                                               <  SRR4302537.4988140/94‑1 (MQ=60)

                                 ACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTC                                                              >  SRR4302537.4242887/1‑94 (MQ=60)

                                     CACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGcag                                                          >  SRR4302537.1091691/1‑91 (MQ=60)

                                     CACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCG                                                          >  SRR4302537.1600377/1‑94 (MQ=60)

                                     CACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCG                                                          <  SRR4302537.6234460/94‑1 (MQ=60)

                                      ACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGA                                                         >  SRR4302537.4509088/1‑94 (MQ=60)

                                      ACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGA                                                         >  SRR4302537.4553809/1‑94 (MQ=60)

                                       CTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGAT                                                        <  SRR4302537.4051753/94‑1 (MQ=60)

                                       CTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGAT                                                        <  SRR4302537.5979266/94‑1 (MQ=60)

                                       CTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGAT                                                        <  SRR4302537.206883/94‑1 (MQ=60)

                                        TTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATG                                                       >  SRR4302537.3352414/1‑94 (MQ=60)

                                        TTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATG                                                       >  SRR4302537.1915754/1‑94 (MQ=60)

                                        TTCCAGCCAGGCAGTGGCGGATCAATATGCCGAATTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAGGGCCAACACCTCGTCGATG                                                       >  SRR4302537.5074144/1‑94 (MQ=60)

                                         TCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGG                                                      <  SRR4302537.2786373/94‑1 (MQ=60)

                                         TCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGG                                                      >  SRR4302537.5188191/1‑94 (MQ=60)

                                          CCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGA                                                     >  SRR4302537.5762432/1‑94 (MQ=60)

                                            AGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATT                                                   <  SRR4302537.2049873/94‑1 (MQ=60)

                                            AGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATT                                                   <  SRR4302537.4830320/94‑1 (MQ=60)

                                             GCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTA                                                  <  SRR4302537.5153382/94‑1 (MQ=60)

                                                 GGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTAC                                              <  SRR4302537.2445674/94‑1 (MQ=60)

                                                 GGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTAC                                              >  SRR4302537.6311654/1‑94 (MQ=60)

                                                 GGCAGTGGCGGATCAATATGACGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTAC                                              <  SRR4302537.5672558/94‑1 (MQ=60)

                                                  GCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACC                                             >  SRR4302537.968840/1‑94 (MQ=60)

                                                   CAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCA                                            <  SRR4302537.4692599/94‑1 (MQ=60)

                                                    AGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCAT                                           >  SRR4302537.2149676/1‑94 (MQ=60)

                                                     GTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATC                                          <  SRR4302537.2869411/94‑1 (MQ=60)

                                                      TGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCA                                         >  SRR4302537.1963958/1‑94 (MQ=60)

                                                        GCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGT                                       <  SRR4302537.1764696/94‑1 (MQ=60)

                                                         CGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTT                                      <  SRR4302537.1407906/94‑1 (MQ=60)

                                                         CGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTT                                      <  SRR4302537.6454513/94‑1 (MQ=60)

                                                          GGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTG                                     <  SRR4302537.760904/94‑1 (MQ=60)

                                                            ATCAATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCG                                   <  SRR4302537.5332374/94‑1 (MQ=60)

                                                              CAATATGCCGACTTCCTGCGCGAAGGTTTCCGAGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTT                                 >  SRR4302537.2627767/1‑94 (MQ=60)

                                                               AATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTA                                >  SRR4302537.4376317/1‑94 (MQ=60)

                                                               AATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTA                                >  SRR4302537.2342039/1‑94 (MQ=60)

                                                               AATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTA                                >  SRR4302537.2766482/1‑94 (MQ=60)

                                                               AATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTA                                <  SRR4302537.3028937/94‑1 (MQ=60)

                                                                ATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTAT                               <  SRR4302537.5274982/94‑1 (MQ=60)

                                                                ATATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTAT                               >  SRR4302537.5560612/1‑94 (MQ=60)

                                                                 TATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAATAGGGCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATG                              >  SRR4302537.2685676/1‑94 (MQ=60)

                                                                 TATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATG                              <  SRR4302537.6407891/94‑1 (MQ=60)

                                                                  ATGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGC                             <  SRR4302537.6430053/94‑1 (MQ=60)

                                                                   TGCCGTCTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCG                            <  SRR4302537.4088635/94‑1 (MQ=60)

                                                                   TGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCG                            <  SRR4302537.6481422/94‑1 (MQ=60)

                                                                   TGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCG                            <  SRR4302537.2600881/94‑1 (MQ=60)

                                                                   TGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCG                            <  SRR4302537.5658320/94‑1 (MQ=60)

                                                                   TGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCG                            >  SRR4302537.225545/1‑94 (MQ=60)

                                                                   TGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCG                            >  SRR4302537.4604650/1‑94 (MQ=60)

                                                                   TGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCG                            >  SRR4302537.1633745/1‑94 (MQ=60)

                                                                   TGCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCGCGTCGATGGATTACTACCATCAGTTGCGTTATGCG                            <  SRR4302537.3964467/94‑1 (MQ=60)

                                                                    GCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGG                           <  SRR4302537.4790582/94‑1 (MQ=60)

                                                                    GCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGG                           <  SRR4302537.4904664/94‑1 (MQ=60)

                                                                    GCCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGG                           <  SRR4302537.3047828/94‑1 (MQ=60)

                                                                     CCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGC                          >  SRR4302537.3535252/1‑94 (MQ=60)

                                                                     CCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGC                          <  SRR4302537.1162323/94‑1 (MQ=60)

                                                                     CCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGC                          >  SRR4302537.5831467/1‑94 (MQ=60)

                                                                     CCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGC                          >  SRR4302537.1713217/1‑94 (MQ=60)

                                                                     CCGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGC                          >  SRR4302537.4936096/1‑94 (MQ=60)

                                                                      CGACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCG                         >  SRR4302537.5843294/1‑94 (MQ=60)

                                                                        ACTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGA                       >  SRR4302537.4447885/1‑94 (MQ=60)

                                                                         CTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAA                      <  SRR4302537.4766918/94‑1 (MQ=60)

                                                                         CTTCCTGCGCGAAGGTTTCCGCGTTGTCACGCAGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAA                      <  SRR4302537.417620/94‑1 (MQ=60)

                                                                          TTCCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAA                     >  SRR4302537.5893045/1‑94 (MQ=60)

                                                                            CCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAA                   >  SRR4302537.5627599/1‑94 (MQ=60)

                                                                            CCTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAA                   >  SRR4302537.3092358/1‑94 (MQ=60)

                                                                             CTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAAT                  >  SRR4302537.1248303/1‑94 (MQ=60)

                                                                             CTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAAT                  <  SRR4302537.6515770/94‑1 (MQ=60)

                                                                             CTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAAT                  <  SRR4302537.1622528/94‑1 (MQ=60)

                                                                             CTGCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAAT                  <  SRR4302537.3227880/94‑1 (MQ=60)

                                                                               GCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCG                >  SRR4302537.109200/1‑94 (MQ=60)

                                                                               GCGCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCG                >  SRR4302537.4096798/1‑94 (MQ=60)

                                                                                 GCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACCCCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCG              <  SRR4302537.5348352/94‑1 (MQ=60)

                                                                                 GCGAAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCG              <  SRR4302537.2681327/94‑1 (MQ=60)

                                                                                  CGAAGCTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGG             <  SRR4302537.2412293/94‑1 (MQ=60)

                                                                                    AAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCG           >  SRR4302537.1961571/1‑94 (MQ=60)

                                                                                    AAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCG           <  SRR4302537.2725420/94‑1 (MQ=60)

                                                                                    AAGGTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCG           >  SRR4302537.797778/1‑94 (MQ=60)

                                                                                       GTTTCCGCGTTGTCGCGCCGAACAAAAAGGCCAACCCCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAA        <  SRR4302537.4175380/94‑1 (MQ=60)

                                                                                       GTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGCAAAATCGCGGCGTAA        >  SRR4302537.3625391/1‑94 (MQ=60)

                                                                                       GTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAA        <  SRR4302537.2678442/94‑1 (MQ=60)

                                                                                       GTTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAA        <  SRR4302537.4059150/94‑1 (MQ=60)

                                                                                        TTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAA       <  SRR4302537.5048438/94‑1 (MQ=60)

                                                                                        TTTCCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAA       >  SRR4302537.4523372/1‑94 (MQ=60)

                                                                                           CCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTC    <  SRR4302537.2177324/94‑1 (MQ=60)

                                                                                           CCGCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTC    <  SRR4302537.7136/94‑1 (MQ=60)

                                                                                             GCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCT  <  SRR4302537.1953134/94‑1 (MQ=60)

AAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCT  >  NC_000913/1961‑2147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`