Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 125,440 C→T F808F (TTC→TTT)  aceE → pyruvate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913125,4400CT98.6% 1617.3 / 1.0 428F808F (TTC→TTT) aceEpyruvate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (4/2);  new base T (216/205);  total (220/208)
Fisher's exact test for biased strand distribution p-value = 6.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 438 total aligned reads displayed.

TGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAACCTG  >  NC_000913/125349‑125530
                                                                                           |                                                                                          
 gcACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCt                                                                                         >  2:7266641/1‑94 (MQ=255)
 gcACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCt                                                                                         <  1:6507957/94‑1 (MQ=255)
  cACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTg                                                                                        <  2:8479708/94‑1 (MQ=255)
   aCCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGa                                                                                       >  1:7062853/1‑94 (MQ=255)
    cccGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAg                                                                                      >  1:1397318/1‑94 (MQ=255)
     ccGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGc                                                                                     >  2:296503/1‑94 (MQ=255)
     ccGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGc                                                                                     >  2:7842099/1‑94 (MQ=255)
      cGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCa                                                                                    <  2:519129/94‑1 (MQ=255)
      cGCTGCAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCa                                                                                    <  2:9866551/94‑1 (MQ=255)
       gCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAg                                                                                   >  1:2877189/1‑94 (MQ=255)
        cTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAgg                                                                                  >  1:241795/1‑94 (MQ=255)
        cTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAgg                                                                                  >  1:4781127/1‑94 (MQ=255)
         tGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGt                                                                                 >  1:3813297/1‑94 (MQ=255)
         tGGAAACTCCGCGCATTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGt                                                                                 >  2:6101350/1‑94 (MQ=255)
          ggAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTc                                                                                >  1:10518246/1‑94 (MQ=255)
           gAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTcc                                                                               <  1:186042/94‑1 (MQ=255)
             aaCTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGt                                                                             <  2:2663661/94‑1 (MQ=255)
             aaCTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGt                                                                             >  1:6074935/1‑94 (MQ=255)
             aaCTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGt                                                                             >  1:4616714/1‑94 (MQ=255)
               cTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTAc                                                                           >  1:6960103/1‑94 (MQ=255)
               cTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTAc                                                                           >  2:6658383/1‑94 (MQ=255)
               cTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTAc                                                                           <  2:4351155/94‑1 (MQ=255)
               cTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTAc                                                                           >  2:4763801/1‑94 (MQ=255)
                 ccGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACtt                                                                         <  1:8236754/94‑1 (MQ=255)
                  cgcgcgTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTa                                                                        <  2:3300087/94‑1 (MQ=255)
                   gcgcgTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTAc                                                                       >  1:7445666/1‑94 (MQ=255)
                    cgcgTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACg                                                                      <  2:8534302/94‑1 (MQ=255)
                     gcgTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGt                                                                     >  2:111910/1‑94 (MQ=255)
                     gcgTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGt                                                                     <  2:3676522/94‑1 (MQ=255)
                      cgTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTa                                                                    <  1:6231565/94‑1 (MQ=255)
                       gTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTAc                                                                   >  1:8900014/1‑94 (MQ=255)
                        ttCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTAcc                                                                  <  2:2833797/94‑1 (MQ=255)
                        ttCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTAcc                                                                  >  2:6348667/1‑94 (MQ=255)
                        ttCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTAcc                                                                  <  1:3052758/94‑1 (MQ=255)
                         tCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCg                                                                 >  2:8250600/1‑94 (MQ=255)
                         tCCGTATATCGCCCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGCCCGTAATTACGTACCg                                                                 >  2:9461230/1‑94 (MQ=255)
                          ccGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCgg                                                                >  2:1894411/1‑94 (MQ=255)
                           cGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGc                                                               >  2:391340/1‑94 (MQ=255)
                            gTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCt                                                              <  2:5305812/94‑1 (MQ=255)
                             tataTCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTg                                                             <  1:2743083/94‑1 (MQ=255)
                             tataTCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTg                                                             >  1:8822221/1‑94 (MQ=255)
                              atatCGCTCAGGTGATGAACGACGCTCCGGCATGGGCATCTACAGACTATATGAAACTGTTTGCTGAGCAGGTGCGTACTTACGTACGGGTTga                                                            >  2:6921402/1‑94 (MQ=255)
                              atatCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTga                                                            >  1:1241538/1‑94 (MQ=255)
                              atatCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTga                                                            <  2:360078/94‑1 (MQ=255)
                               tatCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTgac                                                           <  2:5548510/94‑1 (MQ=255)
                                atCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTgacg                                                          <  2:312742/94‑1 (MQ=255)
                                   gCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGCCTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACt                                                       >  1:6746981/1‑94 (MQ=255)
                                      cAGGTGATGAACGCCGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACCACtcac                                                    >  1:5517965/1‑91 (MQ=255)
                                      cAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTAcc                                                    <  1:6818539/94‑1 (MQ=255)
                                      cAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTAcc                                                    <  1:8557990/94‑1 (MQ=255)
                                      cAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTAcc                                                    <  1:2669259/94‑1 (MQ=255)
                                      cAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTAcc                                                    <  2:1633544/94‑1 (MQ=255)
                                         gTGATGAACGACGCTCCGGAAGTGGCATATACCGCCTATATGAAACTGTTCGCTGAGCAGGTCGGTACTTACGTACCGGCTGACGACTACcggg                                                 >  1:8468790/1‑92 (MQ=255)
                                             tgaACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTAACCCGTACt                                             >  1:10248819/1‑94 (MQ=255)
                                               aaCGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTgg                                           <  2:9171755/94‑1 (MQ=255)
                                                  gacgCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTa                                        >  2:2472467/1‑94 (MQ=255)
                                                   acgCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTCCTGGGTAc                                       >  1:4108241/1‑94 (MQ=255)
                                                     gCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTg                                     >  2:2303175/1‑94 (MQ=255)
                                                         cGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATgg                                 >  2:4188441/1‑94 (MQ=255)
                                                          ggCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGc                                >  1:7189674/1‑94 (MQ=255)
                                                           gCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCt                               <  1:4301871/94‑1 (MQ=255)
                                                           gCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCt                               <  2:10028516/94‑1 (MQ=255)
                                                            cAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCtt                              >  1:1080159/1‑94 (MQ=255)
                                                             aGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTc                             <  1:198982/94‑1 (MQ=255)
                                                             aGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTc                             >  1:5239919/1‑94 (MQ=255)
                                                               tGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCgg                           <  2:10167446/94‑1 (MQ=255)
                                                               tGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGGACTGATGGCTTCgg                           >  2:6003242/1‑94 (MQ=255)
                                                                 gCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTc                         <  2:4063419/94‑1 (MQ=255)
                                                                 gCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTc                         >  2:9662470/1‑94 (MQ=255)
                                                                 gCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCAGTc                         <  1:6175682/94‑1 (MQ=255)
                                                                  cATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCg                        >  1:9359664/1‑94 (MQ=255)
                                                                     cTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTc                     >  1:8112748/1‑94 (MQ=255)
                                                                     cTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTc                     >  1:3381913/1‑94 (MQ=255)
                                                                      tACCGNCNATNTGAAACTGTTNGNTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTcc                    <  2:9312443/94‑1 (MQ=255)
                                                                      tACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGtttc                    >  2:10531225/1‑92 (MQ=255)
                                                                      tACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTcc                    >  2:6234244/1‑94 (MQ=255)
                                                                       aCCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCg                   <  1:10355002/94‑1 (MQ=255)
                                                                          gACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACa                <  1:9186826/94‑1 (MQ=255)
                                                                           aCTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTg                                                             >  1:7728717/1‑48 (MQ=255)
                                                                           aCTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTg                                                             <  2:7728717/48‑1 (MQ=255)
                                                                           aCTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGGCGTTTCgcgag               >  1:1162138/1‑90 (MQ=255)
                                                                            cTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGc              <  2:1979240/94‑1 (MQ=255)
                                                                             tataTGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGcc             >  1:7917320/1‑94 (MQ=255)
                                                                              atatGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCg            >  2:4573425/1‑94 (MQ=255)
                                                                               tatGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCgg           >  1:4527421/1‑93 (MQ=255)
                                                                               tatGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCgg           >  2:8043244/1‑93 (MQ=255)
                                                                                atGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTg          <  1:4027865/94‑1 (MQ=255)
                                                                                  gAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTgag        >  2:9046740/1‑94 (MQ=255)
                                                                                   aaaCTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTgaga       >  2:10346400/1‑94 (MQ=255)
                                                                                   aaaCTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTgaga       >  1:5947891/1‑94 (MQ=255)
                                                                                    aaCTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGaa      <  1:6602774/94‑1 (MQ=255)
                                                                                    aaCTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGaa      >  1:1563012/1‑94 (MQ=255)
                                                                                    aaCTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCCTACTGGGTACTGATTGCTTCGGTTGGTCCGACAGACGCGAGaa      >  1:7105429/1‑94 (MQ=255)
                                                                                     aCTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAAc     <  2:9388616/94‑1 (MQ=255)
                                                                                     aCTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAAc     <  1:864157/94‑1 (MQ=255)
                                                                                      cTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAAcc    <  1:5582933/94‑1 (MQ=255)
                                                                                      cTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAAcc    >  2:480215/1‑94 (MQ=255)
                                                                                       tGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAACCt   >  1:5707943/1‑94 (MQ=255)
                                                                                       tGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAACCt   <  1:4945920/94‑1 (MQ=255)
                                                                                       tGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAACCt   <  2:499625/94‑1 (MQ=255)
                                                                                           |                                                                                          
TGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAACCTG  >  NC_000913/125349‑125530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 237 total aligned reads displayed.
GCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAACCTGCGT  >  NC_000913/125347‑125533
                                                                                             |                                                                                             
GCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTT                                                                                               <  SRR4302545.1027997/94‑1 (MQ=60)
GCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGCCAGTGGCATCTACCGACTATATGAAACTGTTT                                                                                               >  SRR4302545.5490999/1‑94 (MQ=60)
 CTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTG                                                                                              >  SRR4302545.8577810/1‑94 (MQ=60)
  TGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGC                                                                                             >  SRR4302545.623930/1‑94 (MQ=60)
   GCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCT                                                                                            <  SRR4302545.7902337/94‑1 (MQ=60)
   GCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCT                                                                                            <  SRR4302545.6685917/94‑1 (MQ=60)
   GCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCT                                                                                            <  SRR4302545.8996399/94‑1 (MQ=60)
   GCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCT                                                                                            <  SRR4302545.5315962/94‑1 (MQ=60)
    CACCCGCTGGAATCTCCGCGGGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTG                                                                                           <  SRR4302545.8718425/94‑1 (MQ=60)
    CACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTG                                                                                           <  SRR4302545.9723886/94‑1 (MQ=60)
    CACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTG                                                                                           <  SRR4302545.8718426/94‑1 (MQ=60)
    CACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTG                                                                                           <  SRR4302545.8985547/94‑1 (MQ=60)
    CACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTG                                                                                           <  SRR4302545.1137790/94‑1 (MQ=60)
    CACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTG                                                                                           >  SRR4302545.6238161/1‑94 (MQ=60)
    CACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTG                                                                                           <  SRR4302545.9452894/94‑1 (MQ=60)
     ACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGA                                                                                          >  SRR4302545.7258165/1‑94 (MQ=60)
      CCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAG                                                                                         >  SRR4302545.9117469/1‑94 (MQ=60)
      CCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAG                                                                                         >  SRR4302545.1428758/1‑94 (MQ=60)
       CCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGC                                                                                        <  SRR4302545.6719463/94‑1 (MQ=60)
       CCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGC                                                                                        <  SRR4302545.2751237/94‑1 (MQ=60)
        CGCTGGAATCTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCA                                                                                       <  SRR4302545.10692024/94‑1 (MQ=60)
        CGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCA                                                                                       >  SRR4302545.4922480/1‑94 (MQ=60)
        CGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCA                                                                                       <  SRR4302545.693039/94‑1 (MQ=60)
        CGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCA                                                                                       <  SRR4302545.6832387/94‑1 (MQ=60)
         GCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAG                                                                                      <  SRR4302545.3533380/94‑1 (MQ=60)
         GCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAG                                                                                      >  SRR4302545.2946805/1‑94 (MQ=60)
         GCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAG                                                                                      >  SRR4302545.3158735/1‑94 (MQ=60)
         GCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAG                                                                                      >  SRR4302545.7477247/1‑94 (MQ=60)
         GCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAG                                                                                      >  SRR4302545.4548281/1‑94 (MQ=60)
         GCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAG                                                                                      <  SRR4302545.2374596/94‑1 (MQ=60)
         GCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAG                                                                                      <  SRR4302545.2234905/94‑1 (MQ=60)
         GCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAG                                                                                      <  SRR4302545.10680190/94‑1 (MQ=60)
         GCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAG                                                                                      >  SRR4302545.1583853/1‑94 (MQ=60)
          CTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGG                                                                                     >  SRR4302545.246778/1‑94 (MQ=60)
          CTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGG                                                                                     >  SRR4302545.4903370/1‑94 (MQ=60)
           TGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGT                                                                                    >  SRR4302545.1981060/1‑94 (MQ=60)
           TGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGT                                                                                    >  SRR4302545.7502826/1‑94 (MQ=60)
             GAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACCGTTTGCTGAGCAGGTCC                                                                                  >  SRR4302545.10594424/1‑94 (MQ=60)
              AAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCG                                                                                 >  SRR4302545.693926/1‑94 (MQ=60)
              AAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCG                                                                                 >  SRR4302545.5445559/1‑94 (MQ=60)
              AAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTAAGCAGGTCCG                                                                                 >  SRR4302545.327441/1‑94 (MQ=60)
               AACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGT                                                                                >  SRR4302545.6411980/1‑94 (MQ=60)
               AACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGT                                                                                >  SRR4302545.6239888/1‑94 (MQ=60)
               AACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGT                                                                                <  SRR4302545.6345259/94‑1 (MQ=60)
               AACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGT                                                                                >  SRR4302545.4734805/1‑94 (MQ=60)
                ACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTA                                                                               <  SRR4302545.480185/94‑1 (MQ=60)
                 CTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTAC                                                                              >  SRR4302545.5643706/1‑94 (MQ=60)
                 CTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTAC                                                                              >  SRR4302545.7151970/1‑94 (MQ=60)
                 CTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTAC                                                                              >  SRR4302545.9478182/1‑94 (MQ=60)
                 CTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTAC                                                                              <  SRR4302545.9486360/94‑1 (MQ=60)
                 CTCCGCGCGTGCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTAC                                                                              <  SRR4302545.5753188/94‑1 (MQ=60)
                   CCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTT                                                                            <  SRR4302545.8475213/94‑1 (MQ=60)
                   CCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTT                                                                            <  SRR4302545.8475214/94‑1 (MQ=60)
                   CCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTT                                                                            <  SRR4302545.1896985/94‑1 (MQ=60)
                    CGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTA                                                                           >  SRR4302545.9060549/1‑94 (MQ=60)
                     GCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTAC                                                                          >  SRR4302545.2797897/1‑94 (MQ=60)
                     GCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTAC                                                                          >  SRR4302545.7656455/1‑94 (MQ=60)
                     GCGCGTTCCGTATATCGCTAAGGAGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCggtgcctac                                                                          >  SRR4302545.3101961/1‑85 (MQ=60)
                     CCGCGTTCCGTATATCGCTCAGGTGATGAACTACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTAC                                                                          <  SRR4302545.1140610/94‑1 (MQ=60)
                        CGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTA                                                                       <  SRR4302545.6401376/94‑1 (MQ=60)
                         GTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTAC                                                                      <  SRR4302545.775472/94‑1 (MQ=60)
                         GTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTAC                                                                      >  SRR4302545.7168051/1‑94 (MQ=60)
                         GTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTAC                                                                      >  SRR4302545.9158706/1‑94 (MQ=60)
                         GTTCCGTATATCGCTCAGGCGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCACGTCCGTACTTACGTAC                                                                      >  SRR4302545.1534292/1‑94 (MQ=60)
                          TTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACC                                                                     <  SRR4302545.2003626/94‑1 (MQ=60)
                          TTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACC                                                                     <  SRR4302545.5782475/94‑1 (MQ=60)
                          TTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACC                                                                     <  SRR4302545.3127613/94‑1 (MQ=60)
                           TCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCG                                                                    >  SRR4302545.10593435/1‑94 (MQ=60)
                           TCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCG                                                                    <  SRR4302545.9416704/94‑1 (MQ=60)
                            CCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGG                                                                   <  SRR4302545.9417009/94‑1 (MQ=60)
                            CCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGG                                                                   >  SRR4302545.7004987/1‑94 (MQ=60)
                            CCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGG                                                                   <  SRR4302545.4083346/94‑1 (MQ=60)
                            CCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGG                                                                   >  SRR4302545.2774389/1‑94 (MQ=60)
                            CCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCAGTACTTACGTACCGG                                                                   >  SRR4302545.1338711/1‑94 (MQ=60)
                            CCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGACCAGGTCCGTACTTACGTACCGG                                                                   >  SRR4302545.249356/1‑94 (MQ=60)
                             CGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGC                                                                  >  SRR4302545.4091093/1‑94 (MQ=60)
                             CGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGC                                                                  >  SRR4302545.2596215/1‑94 (MQ=60)
                              GTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCT                                                                 >  SRR4302545.8359491/1‑94 (MQ=60)
                              GTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCT                                                                 >  SRR4302545.6374708/1‑94 (MQ=60)
                              GTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCT                                                                 >  SRR4302545.6374707/1‑94 (MQ=60)
                              GTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCT                                                                 <  SRR4302545.1082516/94‑1 (MQ=60)
                               TATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTG                                                                <  SRR4302545.2809242/94‑1 (MQ=60)
                               TATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTG                                                                >  SRR4302545.9078381/1‑94 (MQ=60)
                               TATATCGCTCAGGTGATGAACAACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTG                                                                <  SRR4302545.3709407/94‑1 (MQ=60)
                                ATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGA                                                               >  SRR4302545.1269494/1‑94 (MQ=60)
                                 TATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGAC                                                              <  SRR4302545.7274448/94‑1 (MQ=60)
                                 TATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGAC                                                              <  SRR4302545.4787849/94‑1 (MQ=60)
                                 TATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGAC                                                              <  SRR4302545.8418766/94‑1 (MQ=60)
                                  ATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACG                                                             <  SRR4302545.9558166/94‑1 (MQ=60)
                                     GCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACT                                                          >  SRR4302545.1816199/1‑94 (MQ=60)
                                     GCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACT                                                          >  SRR4302545.4219861/1‑94 (MQ=60)
                                      CTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGCCGACTA                                                         >  SRR4302545.2952352/1‑94 (MQ=60)
                                      CTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTA                                                         <  SRR4302545.7293640/94‑1 (MQ=60)
                                      CTCAGGTGATGAACGACGCTCCAGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTA                                                         <  SRR4302545.7293639/94‑1 (MQ=60)
                                        CAGGTGATGAACGCCGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACcactcac                                                       >  SRR4302545.5663559/1‑87 (MQ=60)
                                        CAGGTGATGAACGACGCTCCGGCAGTTGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACC                                                       <  SRR4302545.8881688/94‑1 (MQ=60)
                                        CAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACC                                                       <  SRR4302545.8805415/94‑1 (MQ=60)
                                        CAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACC                                                       >  SRR4302545.5663560/1‑94 (MQ=60)
                                        CAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACC                                                       <  SRR4302545.5819621/94‑1 (MQ=60)
                                        CAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACC                                                       <  SRR4302545.7005902/94‑1 (MQ=60)
                                        CAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACC                                                       <  SRR4302545.2733560/94‑1 (MQ=60)
                                         AGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCG                                                      >  SRR4302545.7950483/1‑94 (MQ=60)
                                         AGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCA                                                      <  SRR4302545.841141/94‑1 (MQ=60)
                                           GTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCG                                                    >  SRR4302545.4710836/1‑94 (MQ=60)
                                           GTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACCGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCG                                                    <  SRR4302545.6479765/94‑1 (MQ=60)
                                           GTGATGAACGACGCTCCGGAAGTGGCATATACCGCCTATATGAAACTGTTCGCTGAGCAGGTCGGTACTTACGTACCGGCTGACGACTACCGGG                                                    >  SRR4302545.8713531/1‑94 (MQ=60)
                                            TGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGT                                                   >  SRR4302545.9356310/1‑94 (MQ=60)
                                            TGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGT                                                   <  SRR4302545.8228809/94‑1 (MQ=60)
                                             GATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTA                                                  <  SRR4302545.626783/94‑1 (MQ=60)
                                              ATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTAC                                                 >  SRR4302545.7167846/1‑94 (MQ=60)
                                              ATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTAC                                                 <  SRR4302545.4829906/94‑1 (MQ=60)
                                               TGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTAACCCGTACT                                                >  SRR4302545.10551360/1‑94 (MQ=60)
                                                GAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTG                                               <  SRR4302545.10789084/94‑1 (MQ=60)
                                                  ACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCccctactgtg                                             >  SRR4302545.6171182/1‑84 (MQ=60)
                                                    GACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTA                                           >  SRR4302545.1337161/1‑94 (MQ=60)
                                                     ACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTCCTGGGTAC                                          >  SRR4302545.4211056/1‑94 (MQ=60)
                                                     ACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTAC                                          >  SRR4302545.3374467/1‑94 (MQ=60)
                                                       GCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTG                                        >  SRR4302545.6228109/1‑94 (MQ=60)
                                                         TCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGAT                                      <  SRR4302545.4645410/94‑1 (MQ=60)
                                                         TCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGGACTGGGTAacgat                                      >  SRR4302545.4501136/1‑89 (MQ=60)
                                                            GGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGC                                   >  SRR4302545.7389729/1‑94 (MQ=60)
                                                            GGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGC                                   >  SRR4302545.6791163/1‑94 (MQ=60)
                                                             GCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCT                                  <  SRR4302545.7033271/94‑1 (MQ=60)
                                                              CAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTT                                 >  SRR4302545.1103573/1‑94 (MQ=60)
                                                              CAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTT                                 >  SRR4302545.5362806/1‑94 (MQ=60)
                                                               AGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTC                                <  SRR4302545.203138/94‑1 (MQ=60)
                                                               AGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTC                                >  SRR4302545.5375143/1‑94 (MQ=60)
                                                                GTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCG                               <  SRR4302545.5700410/94‑1 (MQ=60)
                                                                 TGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGG                              >  SRR4302545.9089573/1‑94 (MQ=60)
                                                                 TCGCATCTACCGACTATATGAGTCTGTTTGCTGAGCAGGTCCGTACTTGCGTACCGGCTGACGACTCCCGCGTGCTGGGTACTGATGGCTTCGG                              <  SRR4302545.7467228/94‑1 (MQ=60)
                                                                  TGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGT                             <  SRR4302545.3639066/94‑1 (MQ=60)
                                                                  GGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGT                             >  SRR4302545.6443368/1‑94 (MQ=60)
                                                                  GGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGT                             >  SRR4302545.7177353/1‑94 (MQ=60)
                                                                   GCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTC                            <  SRR4302545.1225779/94‑1 (MQ=60)
                                                                   GCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTC                            <  SRR4302545.10915478/94‑1 (MQ=60)
                                                                   GCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCAGTC                            <  SRR4302545.6343763/94‑1 (MQ=60)
                                                                    CATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCG                           <  SRR4302545.2018504/94‑1 (MQ=60)
                                                                    CATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCG                           >  SRR4302545.9634215/1‑94 (MQ=60)
                                                                    CATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCG                           <  SRR4302545.247154/94‑1 (MQ=60)
                                                                    CATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGAACTCGGTACCGCTGGCTTCGGcgc                           >  SRR4302545.8196927/1‑91 (MQ=60)
                                                                     ATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGT                          <  SRR4302545.3026543/94‑1 (MQ=60)
                                                                     ATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGT                          >  SRR4302545.8703153/1‑94 (MQ=60)
                                                                     ATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGT                          >  SRR4302545.4283034/1‑94 (MQ=60)
                                                                     ATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGT                          >  SRR4302545.2886164/1‑94 (MQ=60)
                                                                      TCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTT                         <  SRR4302545.8851265/94‑1 (MQ=60)
                                                                      TCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGGACTGATGGCTTCGGTCGTT                         >  SRR4302545.5305772/1‑94 (MQ=60)
                                                                       CTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTC                        >  SRR4302545.3464335/1‑94 (MQ=60)
                                                                        TACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGTTTCGGTCGTTCC                       >  SRR4302545.6895639/1‑94 (MQ=60)
                                                                        TACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTGGTTTC                       >  SRR4302545.8082288/1‑94 (MQ=60)
                                                                         ACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCG                      <  SRR4302545.4468169/94‑1 (MQ=60)
                                                                         ACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCG                      <  SRR4302545.10660217/94‑1 (MQ=60)
                                                                         ACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCG                      <  SRR4302545.4989098/94‑1 (MQ=60)
                                                                         ACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCG                      <  SRR4302545.5936098/94‑1 (MQ=60)
                                                                         ACCGACTATATGAAACTGTTTGCTGAGCAGGTACGTACTTACGTACCGGCTGACGACTACCGCGGACTGGGTACTGATGGCTTCGGTCGGTCCG                      >  SRR4302545.6985678/1‑94 (MQ=60)
                                                                          CCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGA                     >  SRR4302545.8836877/1‑94 (MQ=60)
                                                                           CGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGAC                    <  SRR4302545.1774569/94‑1 (MQ=60)
                                                                             ACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGAgatcagagatcggaagagcgtcgtgtagggaaagagtgtagatct                  >  SRR4302545.7947473/1‑49 (MQ=60)
                                                                             ACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGgcgtttcgcgag                  >  SRR4302545.1187764/1‑82 (MQ=60)
                                                                             ACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAG                  <  SRR4302545.7360522/94‑1 (MQ=60)
                                                                             ACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAG                  >  SRR4302545.10842313/1‑94 (MQ=60)
                                                                               TATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCC                >  SRR4302545.4066057/1‑94 (MQ=60)
                                                                               TATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCC                >  SRR4302545.8142098/1‑94 (MQ=60)
                                                                               TATATGAAACTGTTTGCTCAGCAGGTCCGTACTTACGTACCTGCTGACGACTACCGCGGACTGGGTACTGATGGCTTCGGccgttgcggcaggc                >  SRR4302545.7700164/1‑80 (MQ=60)
                                                                                ATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGGTTCGGTCGTTCCGACAGCCG               >  SRR4302545.1205601/1‑94 (MQ=60)
                                                                                ATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCG               >  SRR4302545.185706/1‑94 (MQ=60)
                                                                                ATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCG               >  SRR4302545.8607384/1‑94 (MQ=60)
                                                                                ATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCG               <  SRR4302545.8325760/94‑1 (MQ=60)
                                                                                 TATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGT              >  SRR4302545.5845490/1‑94 (MQ=60)
                                                                                 TATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGT              >  SRR4302545.4643413/1‑94 (MQ=60)
                                                                                 TATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGG              >  SRR4302545.4643414/1‑94 (MQ=60)
                                                                                 TATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTAACGGCTGACGAATAACGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGGCGT              >  SRR4302545.4910710/1‑94 (MQ=60)
                                                                                  ATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTG             >  SRR4302545.5907532/1‑94 (MQ=60)
                                                                                  ATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTG             <  SRR4302545.4128668/94‑1 (MQ=60)
                                                                                    GAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAG           <  SRR4302545.4164636/94‑1 (MQ=60)
                                                                                    GAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAG           <  SRR4302545.10849927/94‑1 (MQ=60)
                                                                                    GAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAG           >  SRR4302545.3569946/1‑94 (MQ=60)
                                                                                     AAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGA          >  SRR4302545.1269437/1‑94 (MQ=60)
                                                                                     AAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGA          >  SRR4302545.6108372/1‑94 (MQ=60)
                                                                                     AAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGGGAGA          >  SRR4302545.4161026/1‑94 (MQ=60)
                                                                                     AAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTCCCGACAGCCGTGGGA          <  SRR4302545.664259/94‑1 (MQ=60)
                                                                                      AACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTGGTTCCGGCAGACGTGTGAA         >  SRR4302545.3017587/1‑94 (MQ=60)
                                                                                      AACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGGA         >  SRR4302545.1359132/1‑94 (MQ=60)
                                                                                      AACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAA         >  SRR4302545.5703887/1‑94 (MQ=60)
                                                                                      AACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAA         >  SRR4302545.1599780/1‑94 (MQ=60)
                                                                                      AACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAA         <  SRR4302545.6783563/94‑1 (MQ=60)
                                                                                      AACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCCTACTGGGTACTGATTGCTTCGGTTGGTCCGACAGACGCGAGAA         >  SRR4302545.7302271/1‑94 (MQ=60)
                                                                                      AACTGTTTGCTGAGCAGGGCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAA         <  SRR4302545.6783562/94‑1 (MQ=60)
                                                                                       ACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAAC        <  SRR4302545.883538/94‑1 (MQ=60)
                                                                                        CTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAACC       >  SRR4302545.2911958/1‑94 (MQ=60)
                                                                                         TGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAACCT      <  SRR4302545.5071275/94‑1 (MQ=60)
                                                                                         TGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAACCT      >  SRR4302545.5860900/1‑94 (MQ=60)
                                                                                          GTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGCCAGCCGCGAGAACCTG     >  SRR4302545.4331404/1‑94 (MQ=60)
                                                                                          GTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAACCTG     <  SRR4302545.7016839/94‑1 (MQ=60)
                                                                                          GTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAACCTG     >  SRR4302545.4331405/1‑94 (MQ=60)
                                                                                          GTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTAGGTCGTTCCGACAGCCGTGAGAACCTG     >  SRR4302545.7313060/1‑94 (MQ=60)
                                                                                           TTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAACCTGC    <  SRR4302545.5835045/94‑1 (MQ=60)
                                                                                            TTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAACCTGCG   <  SRR4302545.10205447/94‑1 (MQ=60)
                                                                                            TTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAACCTGCG   <  SRR4302545.10205448/94‑1 (MQ=60)
                                                                                             TGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAACCTGCGT  >  SRR4302545.2042003/1‑94 (MQ=60)
                                                                                             TGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAACCTGCGT  >  SRR4302545.6171756/1‑94 (MQ=60)
                                                                                             |                                                                                             
GCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAACCTGCGT  >  NC_000913/125347‑125533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑