Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 125,440 C→T F808F (TTC→TTT)  aceE → pyruvate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913125,4400CT99.6% 954.5 / ‑5.9 268F808F (TTC→TTT) aceEpyruvate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (140/126);  total (140/128)
Fisher's exact test for biased strand distribution p-value = 4.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 273 total aligned reads displayed.

GCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAACCT  >  NC_000913/125350‑125529
                                                                                          |                                                                                         
 cACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCt                                                                                        <  1:3151586/93‑1 (MQ=255)
 cACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCt                                                                                        <  2:5089079/93‑1 (MQ=255)
  aCCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTg                                                                                       >  1:4969370/1‑93 (MQ=255)
   cccGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGa                                                                                      <  1:2723042/93‑1 (MQ=255)
    ccGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAg                                                                                     <  2:6241902/93‑1 (MQ=255)
    ccGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAg                                                                                     >  1:5784150/1‑93 (MQ=255)
     cGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGc                                                                                    >  2:3570845/1‑93 (MQ=255)
       cTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAg                                                                                  <  2:5784455/93‑1 (MQ=255)
        tGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAgg                                                                                 >  1:2178077/1‑93 (MQ=255)
        tGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACACTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAgg                                                                                 >  1:3840524/1‑93 (MQ=255)
        gggAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAgg                                                                                 <  1:3288304/92‑1 (MQ=255)
         ggAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGt                                                                                <  1:1553194/93‑1 (MQ=255)
         ggAAACTCCGCGCGTTCCGTATATCGCTCAGGGGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGACCAGGt                                                                                >  2:2712695/1‑93 (MQ=255)
          gAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTc                                                                               <  2:1537674/93‑1 (MQ=255)
          gAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTc                                                                               >  2:4331397/1‑93 (MQ=255)
            aaCTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCg                                                                             <  1:2322399/93‑1 (MQ=255)
            aaCTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCg                                                                             >  2:1038198/1‑93 (MQ=255)
               tCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGtgc                                                                          <  1:4112223/93‑3 (MQ=255)
               tCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTAc                                                                          <  1:853585/93‑1 (MQ=255)
                ccGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACt                                                                         <  1:2452125/93‑1 (MQ=255)
                ccGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACt                                                                         >  2:5550658/1‑93 (MQ=255)
                 cgcgcgTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACtt                                                                        <  2:3451890/93‑1 (MQ=255)
                 cgcgcgTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACtt                                                                        <  1:950380/93‑1 (MQ=255)
                  gcgcgTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTa                                                                       <  1:4541277/93‑1 (MQ=255)
                  gcgcgTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTa                                                                       <  1:4237286/93‑1 (MQ=255)
                  gcgcgTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTa                                                                       <  2:5303044/93‑1 (MQ=255)
                  gcgcgTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTa                                                                       <  1:1395499/93‑1 (MQ=255)
                     cgTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTCTTTGCTGAGCAGGTCCGTACTTatga                                                                    >  2:2094715/1‑90 (MQ=255)
                      gTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTa                                                                   >  1:5365083/1‑93 (MQ=255)
                      gTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTa                                                                   >  1:6313585/1‑93 (MQ=255)
                       ttCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTAc                                                                  >  2:2719696/1‑93 (MQ=255)
                       ttCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTAc                                                                  >  1:5141733/1‑93 (MQ=255)
                        tCCGTATATCTCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTAcc                                                                 <  1:2105474/93‑1 (MQ=255)
                        tCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTAcc                                                                 <  1:5474424/93‑1 (MQ=255)
                        tCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTAcc                                                                 >  1:2683608/1‑93 (MQ=255)
                         ccGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCg                                                                >  2:4151041/1‑93 (MQ=255)
                         ccGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCg                                                                >  2:3310675/1‑93 (MQ=255)
                         ccGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCg                                                                >  1:2904645/1‑93 (MQ=255)
                          cGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCgg                                                               <  1:3789898/93‑1 (MQ=255)
                          cGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCgg                                                               <  2:1602403/93‑1 (MQ=255)
                            tataTCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCt                                                             >  1:5313346/1‑93 (MQ=255)
                            tataTCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCt                                                             >  2:1314009/1‑93 (MQ=255)
                               atCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTgac                                                          <  2:3783096/93‑1 (MQ=255)
                               atCGCTCAGGTGATGAACGACGCTCCGGCAGTAGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTgac                                                          <  1:3778028/93‑1 (MQ=255)
                                   cTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACt                                                      >  1:1357878/1‑93 (MQ=255)
                                    tCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTa                                                     <  1:671492/93‑1 (MQ=255)
                                     cAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTAc                                                    <  2:2857336/93‑1 (MQ=255)
                                      aGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTAcc                                                   >  2:4535666/1‑93 (MQ=255)
                                      aGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTAcc                                                   <  1:2140811/93‑1 (MQ=255)
                                       ggTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACcg                                                  >  1:1726915/1‑93 (MQ=255)
                                       ggTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACcg                                                  >  2:2271425/1‑93 (MQ=255)
                                         tgatgaACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACcgcg                                                >  1:832351/1‑93 (MQ=255)
                                         tgatgaACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACcgcg                                                >  1:3225857/1‑93 (MQ=255)
                                          gatgaACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGt                                               >  2:3893945/1‑93 (MQ=255)
                                             gaACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACt                                            >  2:499216/1‑93 (MQ=255)
                                             gaACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACt                                            <  1:3970780/93‑1 (MQ=255)
                                              aaCGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTg                                           >  2:898450/1‑93 (MQ=255)
                                               acgacgCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTgg                                          >  1:1860384/1‑93 (MQ=255)
                                               acgacgCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTgg                                          >  2:3736557/1‑93 (MQ=255)
                                                 gacgCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGt                                        >  2:4194498/1‑93 (MQ=255)
                                                    gCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACt                                     >  1:2970947/1‑93 (MQ=255)
                                                    gCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACt                                     <  1:3378472/93‑1 (MQ=255)
                                                    gCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGAAGACTACCGCGTACTGGGTAcg                                     >  1:1818506/1‑92 (MQ=255)
                                                     cTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTg                                    <  2:245443/93‑1 (MQ=255)
                                                      tCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGa                                   <  1:4012037/93‑1 (MQ=255)
                                                       ccGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGAt                                  >  1:2500508/1‑93 (MQ=255)
                                                        cGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATg                                 <  2:2360353/93‑1 (MQ=255)
                                                           cAGTGGCATCTAGCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCt                              <  1:4998466/93‑1 (MQ=255)
                                                              tGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCg                           <  1:1007354/93‑1 (MQ=255)
                                                               ggCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCgg                          <  1:1512579/93‑1 (MQ=255)
                                                                gCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGt                         >  2:3233476/1‑93 (MQ=255)
                                                                gCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGt                         <  2:805190/93‑1 (MQ=255)
                                                                gCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGt                         <  1:4781718/93‑1 (MQ=255)
                                                                gCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGt                         <  1:5865795/93‑1 (MQ=255)
                                                                  aTCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCg                       <  1:1253048/93‑1 (MQ=255)
                                                                   tCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGt                      >  1:441578/1‑93 (MQ=255)
                                                                    cTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGtt                     <  2:1955950/93‑1 (MQ=255)
                                                                     tACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTc                    <  2:5875454/93‑1 (MQ=255)
                                                                     tACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTc                    >  2:514752/1‑93 (MQ=255)
                                                                       acGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCg                  <  2:4679950/92‑1 (MQ=255)
                                                                        cGACTATATTAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTTTCGCGTACTGGGTACTGATGGCTTCGGTTGTTTCgt                 >  2:5947144/1‑92 (MQ=255)
                                                                        cGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCgc                 >  2:2001046/1‑92 (MQ=255)
                                                                        cGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGa                 >  1:4159242/1‑93 (MQ=255)
                                                                         gACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGAc                >  2:1084281/1‑93 (MQ=255)
                                                                          aCTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACa               >  2:6079407/1‑93 (MQ=255)
                                                                          aCTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACa               >  2:2928221/1‑93 (MQ=255)
                                                                           cTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAg              >  2:4824608/1‑93 (MQ=255)
                                                                            tataTGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGGACTGATGGCTTCGGTCGTTCCGACAGc             >  1:1598067/1‑93 (MQ=255)
                                                                              tatGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCg           >  1:1694003/1‑93 (MQ=255)
                                                                               atGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGCACTGATGGCTTCGGTCGTTCCGGCAGCCgc          >  1:1498605/1‑92 (MQ=255)
                                                                                 gAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGCACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTga        >  1:5945328/1‑93 (MQ=255)
                                                                                  aaaCTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTTCGGCAGCCGTgag       >  2:4583077/1‑93 (MQ=255)
                                                                                  aaaCTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTgag       >  2:4058136/1‑93 (MQ=255)
                                                                                   aaCTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTgaga      <  1:3544701/93‑1 (MQ=255)
                                                                                   aaCTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTCCCGACAGCCGTgaga      <  2:6411818/93‑1 (MQ=255)
                                                                                     cTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAAc    <  1:1302437/93‑1 (MQ=255)
                                                                                     cTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAAc    <  2:6107510/93‑1 (MQ=255)
                                                                                      tGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTTGTTCCGACAGGCGTGACAAcc   >  2:5027832/1‑93 (MQ=255)
                                                                                      tGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAAcc   >  2:2615392/1‑93 (MQ=255)
                                                                                       gTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAACCt  <  1:3643898/93‑1 (MQ=255)
                                                                                          |                                                                                         
GCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAACCT  >  NC_000913/125350‑125529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAACCTGCGT  >  NC_000913/125347‑125533
                                                                                             |                                                                                             
GCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTT                                                                                               <  SRR4302537.2900221/94‑1 (MQ=60)
 CTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTG                                                                                              >  SRR4302537.356987/1‑94 (MQ=60)
   GCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCT                                                                                            <  SRR4302537.2324918/94‑1 (MQ=60)
    CACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTG                                                                                           >  SRR4302537.1591900/1‑94 (MQ=60)
    CACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTG                                                                                           >  SRR4302537.5003619/1‑94 (MQ=60)
    CACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTG                                                                                           >  SRR4302537.182587/1‑94 (MQ=60)
    CACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTG                                                                                           <  SRR4302537.1969097/94‑1 (MQ=60)
    CACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTG                                                                                           <  SRR4302537.3173719/94‑1 (MQ=60)
     CCCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGA                                                                                          <  SRR4302537.5369600/94‑1 (MQ=60)
      CCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAG                                                                                         <  SRR4302537.2742059/94‑1 (MQ=60)
      CCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAG                                                                                         >  SRR4302537.5823804/1‑94 (MQ=60)
      CCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAG                                                                                         <  SRR4302537.3655329/94‑1 (MQ=60)
        CGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCA                                                                                       >  SRR4302537.4254963/1‑94 (MQ=60)
        CGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCA                                                                                       >  SRR4302537.4426648/1‑94 (MQ=60)
        CGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCA                                                                                       >  SRR4302537.4909256/1‑94 (MQ=60)
         GCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAG                                                                                      <  SRR4302537.1294491/94‑1 (MQ=60)
          CTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGG                                                                                     >  SRR4302537.2193429/1‑94 (MQ=60)
          CTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGG                                                                                     <  SRR4302537.5896772/94‑1 (MQ=60)
          CTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACACTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGG                                                                                     >  SRR4302537.3867428/1‑94 (MQ=60)
           TGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGT                                                                                    >  SRR4302537.1954701/1‑94 (MQ=60)
           TGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGT                                                                                    >  SRR4302537.85406/1‑94 (MQ=60)
           GGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGT                                                                                    <  SRR4302537.3311543/94‑1 (MQ=60)
            GGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTC                                                                                   <  SRR4302537.1564029/94‑1 (MQ=60)
            GGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTC                                                                                   >  SRR4302537.2845021/1‑94 (MQ=60)
             GAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCC                                                                                  <  SRR4302537.5971857/94‑1 (MQ=60)
               AACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGT                                                                                <  SRR4302537.2338797/94‑1 (MQ=60)
               AACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGT                                                                                >  SRR4302537.322100/1‑94 (MQ=60)
                ACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCGCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTA                                                                               <  SRR4302537.4054090/94‑1 (MQ=60)
                ACGCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTA                                                                               <  SRR4302537.1517783/94‑1 (MQ=60)
                 CTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTAC                                                                              >  SRR4302537.5163457/1‑94 (MQ=60)
                 CTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTAC                                                                              >  SRR4302537.4352004/1‑94 (MQ=60)
                 CTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTAC                                                                              <  SRR4302537.2130556/94‑1 (MQ=60)
                  TCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTGCT                                                                             <  SRR4302537.4140947/94‑1 (MQ=60)
                  TCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACT                                                                             <  SRR4302537.1542348/94‑1 (MQ=60)
                  TCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACT                                                                             <  SRR4302537.859441/94‑1 (MQ=60)
                   CCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTT                                                                            <  SRR4302537.6079445/94‑1 (MQ=60)
                   CCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTT                                                                            <  SRR4302537.2469378/94‑1 (MQ=60)
                    CGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTA                                                                           <  SRR4302537.956950/94‑1 (MQ=60)
                     GCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTAC                                                                          <  SRR4302537.1405230/94‑1 (MQ=60)
                     GCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTAC                                                                          <  SRR4302537.4266760/94‑1 (MQ=60)
                     GCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTAC                                                                          <  SRR4302537.4572712/94‑1 (MQ=60)
                      CGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACG                                                                         <  SRR4302537.1641745/94‑1 (MQ=60)
                        CGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTA                                                                       >  SRR4302537.5401799/1‑94 (MQ=60)
                        CGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTA                                                                       >  SRR4302537.6356798/1‑94 (MQ=60)
                         GTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTAC                                                                      >  SRR4302537.1681924/1‑94 (MQ=60)
                         GTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTAC                                                                      >  SRR4302537.5177161/1‑94 (MQ=60)
                          TTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACC                                                                     >  SRR4302537.2702344/1‑94 (MQ=60)
                           TCCGTATATCTCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCG                                                                    <  SRR4302537.2120315/94‑1 (MQ=60)
                           TCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCG                                                                    <  SRR4302537.5511785/94‑1 (MQ=60)
                           TCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCG                                                                    >  SRR4302537.2925050/1‑94 (MQ=60)
                           TCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCG                                                                    >  SRR4302537.2437942/1‑94 (MQ=60)
                             CGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGC                                                                  <  SRR4302537.3816497/94‑1 (MQ=60)
                              GTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCT                                                                 <  SRR4302537.5017266/94‑1 (MQ=60)
                              GTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCT                                                                 >  SRR4302537.5349838/1‑94 (MQ=60)
                                 TATCTCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGAC                                                              <  SRR4302537.2815201/94‑1 (MQ=60)
                                 TATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGAC                                                              >  SRR4302537.3979847/1‑94 (MQ=60)
                                 AATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGAC                                                              <  SRR4302537.1194510/94‑1 (MQ=60)
                                  ATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACG                                                             <  SRR4302537.1002134/94‑1 (MQ=60)
                                  ATCGCTCAGGTGATGAACGACGCTCCGGCAGTAGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACG                                                             <  SRR4302537.3804558/94‑1 (MQ=60)
                                     GCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACT                                                          >  SRR4302537.1367333/1‑94 (MQ=60)
                                     GCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACT                                                          <  SRR4302537.692959/94‑1 (MQ=60)
                                      CTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTC                                                         >  SRR4302537.4709018/1‑94 (MQ=60)
                                      CTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTA                                                         >  SRR4302537.4213467/1‑94 (MQ=60)
                                      CTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTA                                                         >  SRR4302537.3397351/1‑94 (MQ=60)
                                      CTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTA                                                         >  SRR4302537.6490593/1‑94 (MQ=60)
                                       TCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTAC                                                        <  SRR4302537.676085/94‑1 (MQ=60)
                                         AGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCG                                                      >  SRR4302537.1738979/1‑94 (MQ=60)
                                         AGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCG                                                      <  SRR4302537.2155880/94‑1 (MQ=60)
                                         AGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCG                                                      >  SRR4302537.130542/1‑94 (MQ=60)
                                         AGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCG                                                      <  SRR4302537.3259766/94‑1 (MQ=60)
                                         AGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCG                                                      <  SRR4302537.1718893/94‑1 (MQ=60)
                                          GGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGC                                                     >  SRR4302537.2390585/1‑94 (MQ=60)
                                           GTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCG                                                    <  SRR4302537.1481997/94‑1 (MQ=60)
                                           GTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCG                                                    >  SRR4302537.3248559/1‑94 (MQ=60)
                                           GTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCG                                                    >  SRR4302537.838044/1‑94 (MQ=60)
                                            TGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGT                                                   <  SRR4302537.1735247/94‑1 (MQ=60)
                                             GATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTA                                                  >  SRR4302537.5456718/1‑94 (MQ=60)
                                              ATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTAC                                                 >  SRR4302537.3830473/1‑94 (MQ=60)
                                               TGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACT                                                <  SRR4302537.5377888/94‑1 (MQ=60)
                                                GAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTG                                               <  SRR4302537.3998569/94‑1 (MQ=60)
                                                 AACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGG                                              >  SRR4302537.3949484/1‑94 (MQ=60)
                                                 AACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGG                                              >  SRR4302537.4472715/1‑94 (MQ=60)
                                                 AACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGG                                              >  SRR4302537.1873338/1‑94 (MQ=60)
                                                   CGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGT                                            <  SRR4302537.887330/94‑1 (MQ=60)
                                                   CGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGT                                            <  SRR4302537.1751562/94‑1 (MQ=60)
                                                     ACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTAC                                          <  SRR4302537.2725627/94‑1 (MQ=60)
                                                      CGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACT                                         >  SRR4302537.2991770/1‑94 (MQ=60)
                                                      CGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGAAGACTACCGCGTACTGGGTACG                                         >  SRR4302537.1831179/1‑94 (MQ=60)
                                                       GCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTG                                        <  SRR4302537.3402412/94‑1 (MQ=60)
                                                       GCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTG                                        <  SRR4302537.3780391/94‑1 (MQ=60)
                                                       GCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTG                                        >  SRR4302537.2257155/1‑94 (MQ=60)
                                                        CTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGA                                       <  SRR4302537.5171406/94‑1 (MQ=60)
                                                         TCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGAT                                      >  SRR4302537.2518101/1‑94 (MQ=60)
                                                         TCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGAT                                      >  SRR4302537.6492656/1‑94 (MQ=60)
                                                         TCCGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGAT                                      <  SRR4302537.4040131/94‑1 (MQ=60)
                                                           CGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGG                                    <  SRR4302537.2940236/94‑1 (MQ=60)
                                                           CGGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGG                                    <  SRR4302537.3617054/94‑1 (MQ=60)
                                                            GGCAGTGGCATCTACCGACTATATGAAGCTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGC                                   <  SRR4302537.3262394/94‑1 (MQ=60)
                                                            GGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGC                                   >  SRR4302537.5540315/1‑94 (MQ=60)
                                                            GGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGC                                   >  SRR4302537.2496516/1‑94 (MQ=60)
                                                            GGCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGC                                   <  SRR4302537.4182220/94‑1 (MQ=60)
                                                             GCAGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCT                                  >  SRR4302537.3453961/1‑94 (MQ=60)
                                                              CAGTGGCATCTAGCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTT                                 <  SRR4302537.5032909/94‑1 (MQ=60)
                                                               AGTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTC                                <  SRR4302537.1509300/94‑1 (MQ=60)
                                                                GTGGCATCTACCGACTATATGAAACTGTTTTCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGAGGGCTTCG                               >  SRR4302537.2585152/1‑94 (MQ=60)
                                                                GTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCG                               >  SRR4302537.2186667/1‑94 (MQ=60)
                                                                GTGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCG                               <  SRR4302537.1328136/94‑1 (MQ=60)
                                                                 TGGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGG                              <  SRR4302537.1014307/94‑1 (MQ=60)
                                                                 GGGCATCTCCCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGG                              <  SRR4302537.1322606/94‑1 (MQ=60)
                                                                  GGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGT                             <  SRR4302537.1523150/94‑1 (MQ=60)
                                                                  GGCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGAAGACTACCGCGTACTGGGTACTGTTGGCTTCGGG                             >  SRR4302537.2704045/1‑94 (MQ=60)
                                                                   GCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTC                            <  SRR4302537.5906037/94‑1 (MQ=60)
                                                                   GCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTC                            <  SRR4302537.4814727/94‑1 (MQ=60)
                                                                   GCATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTAATGATGGCTTCGGTC                            >  SRR4302537.5931344/1‑94 (MQ=60)
                                                                    CATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCG                           <  SRR4302537.1427616/94‑1 (MQ=60)
                                                                    CATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCG                           >  SRR4302537.5650835/1‑94 (MQ=60)
                                                                    CATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCG                           <  SRR4302537.5265352/94‑1 (MQ=60)
                                                                     ATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGT                          <  SRR4302537.1261767/94‑1 (MQ=60)
                                                                     ATCTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGT                          >  SRR4302537.444644/1‑94 (MQ=60)
                                                                       CTACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTC                        <  SRR4302537.4605819/94‑1 (MQ=60)
                                                                        TACCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCC                       <  SRR4302537.5320953/94‑1 (MQ=60)
                                                                         ACCGACTATATGAAACTGTTTGCTGAGCATGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCG                      <  SRR4302537.1401195/94‑1 (MQ=60)
                                                                          CCGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGA                     >  SRR4302537.4188288/1‑94 (MQ=60)
                                                                           CGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGAC                    >  SRR4302537.1557678/1‑94 (MQ=60)
                                                                           CGACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGCTCCGAC                    >  SRR4302537.867025/1‑94 (MQ=60)
                                                                             ACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAG                  >  SRR4302537.4143577/1‑94 (MQ=60)
                                                                             ACTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAG                  >  SRR4302537.3999350/1‑94 (MQ=60)
                                                                              CTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGC                 >  SRR4302537.2925165/1‑94 (MQ=60)
                                                                              CTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGC                 <  SRR4302537.1838387/94‑1 (MQ=60)
                                                                              CTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGGCGTTCCGACAGC                 >  SRR4302537.4014454/1‑94 (MQ=60)
                                                                              CTATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGGACTGATGGCTTCGGTCGTTCCGACAGC                 >  SRR4302537.1609232/1‑94 (MQ=60)
                                                                               TATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCC                <  SRR4302537.3814458/94‑1 (MQ=60)
                                                                               TATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCC                <  SRR4302537.3072258/94‑1 (MQ=60)
                                                                               TATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCC                >  SRR4302537.3459985/1‑94 (MQ=60)
                                                                                ATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCG               >  SRR4302537.1705858/1‑94 (MQ=60)
                                                                                ATATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCG               >  SRR4302537.247911/1‑94 (MQ=60)
                                                                                 TATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGCACTGATGGCTTCGGTCGTTCCGGCAGCCGC              >  SRR4302537.1509064/1‑94 (MQ=60)
                                                                                  ATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTTGGGCGTTCCGACAGCCGTG             >  SRR4302537.3760036/1‑94 (MQ=60)
                                                                                  ATGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTG             <  SRR4302537.4874366/94‑1 (MQ=60)
                                                                                   TGAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGCACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGA            >  SRR4302537.5986135/1‑94 (MQ=60)
                                                                                    TAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAG           <  SRR4302537.3202196/94‑1 (MQ=60)
                                                                                    GAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAG           <  SRR4302537.2138313/94‑1 (MQ=60)
                                                                                    GAAACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAG           >  SRR4302537.383963/1‑94 (MQ=60)
                                                                                      AACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAA         <  SRR4302537.3569740/94‑1 (MQ=60)
                                                                                       ACTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAAC        >  SRR4302537.72931/1‑94 (MQ=60)
                                                                                       ACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAAC        <  SRR4302537.5846303/94‑1 (MQ=60)
                                                                                        CTGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAACC       <  SRR4302537.1311491/94‑1 (MQ=60)
                                                                                         TGTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGCTACTGATGGCTTCGGTCGTTTCGACAGCCGTGAGgagct      >  SRR4302537.2372663/1‑89 (MQ=60)
                                                                                          GTTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAACCTG     <  SRR4302537.3669558/94‑1 (MQ=60)
                                                                                           TTTGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAACCTGC    <  SRR4302537.2940599/94‑1 (MQ=60)
                                                                                             TGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAACCTGCGT  <  SRR4302537.3216123/94‑1 (MQ=60)
                                                                                             TGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAACCTGCGT  <  SRR4302537.2006666/94‑1 (MQ=60)
                                                                                             |                                                                                             
GCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAACCTGCGT  >  NC_000913/125347‑125533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑