Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 126,053 T→C V120A (GTG→GCG)  aceF → pyruvate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913126,0530TC98.7% 1331.6 / 1.5 392V120A (GTG→GCG) aceFpyruvate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (2/3);  new base C (179/207);  total (182/210)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

Only 100 of 405 total aligned reads displayed.

GAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCC  >  NC_000913/125962‑126143
                                                                                           |                                                                                          
 agaagaAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGAc                                                                                         >  2:9590684/1‑94 (MQ=255)
 agaagaAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGAc                                                                                         <  2:8628022/94‑1 (MQ=255)
 agaagaAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAATTGAAGAGAc                                                                                         >  1:2849644/1‑94 (MQ=255)
      aaagaaGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGaaa                                                                                    >  1:120403/1‑94 (MQ=255)
       aagaagCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAAt                                                                                   >  2:2408911/1‑94 (MQ=255)
       aagaagCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAAt                                                                                   <  1:5135667/94‑1 (MQ=255)
         gaagCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATcc                                                                                 <  1:5886841/94‑1 (MQ=255)
                cTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGaa                                                                          <  2:10269537/94‑1 (MQ=255)
                   cGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGCAGCGACCGAAATCCTGGTGAAAGt                                                                       >  2:10197647/1‑94 (MQ=255)
                   cGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGt                                                                       >  1:10117178/1‑94 (MQ=255)
                   cGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGt                                                                       >  1:9637694/1‑94 (MQ=255)
                    ggCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGtt                                                                      <  2:7775448/94‑1 (MQ=255)
                    ggCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGtt                                                                      <  2:6523673/94‑1 (MQ=255)
                     gcagcagcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTg                                                                     <  1:1372854/94‑1 (MQ=255)
                     gcagcagcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTg                                                                     <  1:7508320/94‑1 (MQ=255)
                      cagcagcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTgg                                                                    >  2:217446/1‑94 (MQ=255)
                      cagcagcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTgg                                                                    <  1:10544025/94‑1 (MQ=255)
                      cagcagcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTgg                                                                    >  1:3667915/1‑94 (MQ=255)
                      cagcagcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTgg                                                                    <  1:8328440/94‑1 (MQ=255)
                      cagcagcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTgg                                                                    >  1:4361968/1‑94 (MQ=255)
                        gcagcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCg                                                                  <  2:10071470/94‑1 (MQ=255)
                        gcagcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCg                                                                  <  2:7485886/94‑1 (MQ=255)
                          agcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGAt                                                                >  1:8523785/1‑94 (MQ=255)
                           gcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATa                                                               >  1:6699255/1‑94 (MQ=255)
                           gcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATa                                                               <  1:8857389/94‑1 (MQ=255)
                           gcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATa                                                               <  2:5236482/94‑1 (MQ=255)
                            caCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATaa                                                              >  2:2093791/1‑94 (MQ=255)
                            caCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATaa                                                              >  1:7109925/1‑94 (MQ=255)
                             aCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATaaa                                                             <  2:5730907/94‑1 (MQ=255)
                             aCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATaaa                                                             >  1:6828723/1‑94 (MQ=255)
                             aCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGATGGCGATaaa                                                             >  2:5104/1‑94 (MQ=255)
                               cAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGt                                                           <  1:9144656/94‑1 (MQ=255)
                                aGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGtt                                                          <  2:5466831/94‑1 (MQ=255)
                                 gCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTg                                                         <  2:2355353/94‑1 (MQ=255)
                                 gCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTg                                                         <  1:6082282/94‑1 (MQ=255)
                                    gCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAg                                                      <  1:8664225/94‑1 (MQ=255)
                                    gCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAg                                                      <  1:4207636/94‑1 (MQ=255)
                                     cTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGc                                                     <  2:3553853/94‑1 (MQ=255)
                                     cTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGc                                                     >  1:2057320/1‑94 (MQ=255)
                                      tGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCt                                                    <  2:4081842/94‑1 (MQ=255)
                                       gcggcgGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTg                                                   <  2:3611472/94‑1 (MQ=255)
                                          gcggcAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAAc                                                <  2:2887375/94‑1 (MQ=255)
                                          gcggcAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAAc                                                >  1:483638/1‑94 (MQ=255)
                                          gcggcAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAAc                                                >  2:94505/1‑94 (MQ=255)
                                           cggcAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACa                                               >  1:1080520/1‑94 (MQ=255)
                                           cggcAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACa                                               <  1:7813163/94‑1 (MQ=255)
                                           cggcAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACa                                               <  2:4465087/94‑1 (MQ=255)
                                            ggcAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAg                                              <  2:6192436/94‑1 (MQ=255)
                                            ggcAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAg                                              >  2:7342931/1‑94 (MQ=255)
                                             gcAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGt                                             <  2:1666519/94‑1 (MQ=255)
                                             gcAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGt                                             <  2:6056524/94‑1 (MQ=255)
                                              cAAAAGACGTTAACGTTCCGGATATCGGCAGCGCCGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGATGCACAGtg                                            >  2:3165386/1‑93 (MQ=25)
                                              cAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTc                                            >  1:8423998/1‑94 (MQ=255)
                                               aaaaGACGTTAACGTTCCGGATATCGGCAGCGACGACGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCg                                           <  2:6719988/94‑1 (MQ=255)
                                               aaaaGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCg                                           >  1:2435775/1‑94 (MQ=255)
                                                 aaGACGTTACCGTTCCGGATATCGGCAGCGACGCAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGAGATCAAGTTGAAGCTGAACAGTCGCt                                         <  1:9411461/94‑1 (MQ=25)
                                                  aGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTg                                        >  1:3393461/1‑94 (MQ=255)
                                                  aGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTg                                        <  2:3476530/94‑1 (MQ=255)
                                                  aGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTg                                        <  2:8240891/94‑1 (MQ=255)
                                                  aGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTg                                        >  1:8732912/1‑94 (MQ=255)
                                                  aGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTg                                        <  1:8106221/94‑1 (MQ=255)
                                                   gACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGa                                       >  1:9096512/1‑94 (MQ=255)
                                                    aCGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGAt                                      >  2:7259432/1‑94 (MQ=255)
                                                     cGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGGTGAAGCTGAACAGTCGCTGATc                                     >  2:9374202/1‑94 (MQ=255)
                                                      gTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCa                                    <  1:3213330/94‑1 (MQ=255)
                                                       ttAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCAc                                   <  2:7932171/94‑1 (MQ=255)
                                                         aaCGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCg                                 >  1:1760577/1‑94 (MQ=255)
                                                          aCGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGt                                <  2:10215625/94‑1 (MQ=255)
                                                           cGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTa                               <  1:9270290/94‑1 (MQ=255)
                                                            gTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAg                              >  1:3125725/1‑94 (MQ=255)
                                                            gTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAg                              <  2:4793613/94‑1 (MQ=255)
                                                              tCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGaa                            <  2:8037694/94‑1 (MQ=255)
                                                              tCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGaa                            <  2:5590993/94‑1 (MQ=255)
                                                              tCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGaa                            <  1:3980263/94‑1 (MQ=255)
                                                               ccGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAg                           <  1:4658270/94‑1 (MQ=255)
                                                                cGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAgg                          >  2:7038858/1‑94 (MQ=255)
                                                                    tatCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGAc                      <  2:1211190/94‑1 (MQ=255)
                                                                     atCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACa                     <  2:3802380/94‑1 (MQ=255)
                                                                     atCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACa                     >  1:2654462/1‑94 (MQ=255)
                                                                      tCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACaa                    <  2:15039/94‑1 (MQ=255)
                                                                      tCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACaa                    <  1:374495/94‑1 (MQ=255)
                                                                      tCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACaa                    <  1:3492698/94‑1 (MQ=255)
                                                                       cGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACCGTCGCTGATCACCGTAGAAGGCGACAAg                   <  1:7392139/94‑1 (MQ=255)
                                                                        ggCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAgg                  >  2:7122236/1‑94 (MQ=255)
                                                                          cAGCGCCGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCt                >  1:1330896/1‑94 (MQ=255)
                                                                          cAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCt                <  2:10574911/94‑1 (MQ=255)
                                                                           aGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCtt               >  2:1979437/1‑94 (MQ=255)
                                                                           aGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCtt               <  2:8490996/94‑1 (MQ=255)
                                                                             cgacgaAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCt             <  2:2646673/94‑1 (MQ=255)
                                                                             cgacgaAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCt             <  1:6947020/94‑1 (MQ=255)
                                                                              gacgaAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTa            >  2:11169/1‑94 (MQ=255)
                                                                              gacgaAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTa            >  2:8912370/1‑94 (MQ=255)
                                                                                cgaAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATg          >  1:6559252/1‑94 (MQ=255)
                                                                                 gaAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATgg         >  1:6350563/1‑94 (MQ=255)
                                                                                   aGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGaa       >  1:10197546/1‑94 (MQ=255)
                                                                                     ttGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGt     >  1:10303895/1‑94 (MQ=255)
                                                                                     ttGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGt     <  1:9812901/94‑1 (MQ=255)
                                                                                     gtGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGt     <  1:10013508/93‑1 (MQ=255)
                                                                                      tGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGtt    <  1:5646388/94‑1 (MQ=255)
                                                                                        aaGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTcc  >  2:6834749/1‑94 (MQ=255)
                                                                                           |                                                                                          
GAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCC  >  NC_000913/125962‑126143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 230 total aligned reads displayed.
AAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGC  >  NC_000913/125960‑126146
                                                                                             |                                                                                             
AAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGC                                                                                               <  SRR4302545.6779447/94‑1 (MQ=60)
AAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGC                                                                                               >  SRR4302545.4524642/1‑94 (MQ=60)
 AGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCG                                                                                              >  SRR4302545.7166704/1‑94 (MQ=60)
   AGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGAC                                                                                            <  SRR4302545.2324310/94‑1 (MQ=60)
   AGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGAC                                                                                            <  SRR4302545.7612241/94‑1 (MQ=60)
   AGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAATTGAAGAGAC                                                                                            >  SRR4302545.2918542/1‑94 (MQ=60)
    GAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACC                                                                                           <  SRR4302545.9583355/94‑1 (MQ=60)
     AAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCG                                                                                          <  SRR4302545.4706509/94‑1 (MQ=60)
      AGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGA                                                                                         >  SRR4302545.10649821/1‑94 (MQ=60)
      AGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGA                                                                                         <  SRR4302545.1024168/94‑1 (MQ=60)
       GAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGCCGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAA                                                                                        <  SRR4302545.3821947/94‑1 (MQ=60)
       GAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAA                                                                                        <  SRR4302545.9068136/94‑1 (MQ=60)
       GAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGAGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAA                                                                                        <  SRR4302545.3025686/94‑1 (MQ=60)
       GAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGAGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAA                                                                                        <  SRR4302545.3025688/94‑1 (MQ=60)
        AAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAA                                                                                       >  SRR4302545.123134/1‑94 (MQ=60)
        AAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAA                                                                                       <  SRR4302545.6976294/94‑1 (MQ=60)
         AAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAAT                                                                                      <  SRR4302545.5267520/94‑1 (MQ=60)
         AAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAAT                                                                                      <  SRR4302545.5267519/94‑1 (MQ=60)
         AAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAAT                                                                                      <  SRR4302545.6035152/94‑1 (MQ=60)
         AAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAAT                                                                                      <  SRR4302545.924297/94‑1 (MQ=60)
           GAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCC                                                                                    <  SRR4302545.6045525/94‑1 (MQ=60)
           GAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCAGGATATCGGCAGCGACGAAAGTGAAGCGACCGAAATCC                                                                                    >  SRR4302545.4654258/1‑94 (MQ=60)
            AAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCCAAAGACGTTAACGTTCCGGATATCGGCAGCGACGCAGTTGAAGCGACCGGAATCCT                                                                                   >  SRR4302545.8665609/1‑94 (MQ=60)
            AAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCT                                                                                   <  SRR4302545.3411160/94‑1 (MQ=60)
             AGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGGCCGAAATCCTG                                                                                  >  SRR4302545.9107975/1‑94 (MQ=60)
             AGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTG                                                                                  <  SRR4302545.7334050/94‑1 (MQ=60)
             AGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTG                                                                                  <  SRR4302545.7040833/94‑1 (MQ=60)
               CAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGT                                                                                <  SRR4302545.1315369/94‑1 (MQ=60)
                AGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTG                                                                               >  SRR4302545.9386376/1‑94 (MQ=60)
                AGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTG                                                                               >  SRR4302545.10932421/1‑94 (MQ=60)
                AGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTG                                                                               >  SRR4302545.7202980/1‑94 (MQ=60)
                AGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGGG                                                                               >  SRR4302545.7235628/1‑94 (MQ=60)
                AGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGGTCCGGATATCGGCAGGGGCGGAGTTGACGCGACCGGAATCCTGGTG                                                                               >  SRR4302545.1409275/1‑94 (MQ=60)
                 GCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGA                                                                              >  SRR4302545.10674280/1‑94 (MQ=60)
                  CTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTTCGGATATCGACAGCGACGAAGTTTTAGCCACCGAAATCCTGGTGCA                                                                             >  SRR4302545.9295549/1‑94 (MQ=60)
                   TCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAA                                                                            >  SRR4302545.3601453/1‑94 (MQ=60)
                   TCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAA                                                                            <  SRR4302545.9956867/94‑1 (MQ=60)
                    CCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAG                                                                           >  SRR4302545.3245849/1‑94 (MQ=60)
                    CCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAG                                                                           >  SRR4302545.5968536/1‑94 (MQ=60)
                     CGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGT                                                                          >  SRR4302545.9921828/1‑94 (MQ=60)
                     CGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGT                                                                          >  SRR4302545.10416397/1‑94 (MQ=60)
                      GGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTG                                                                         >  SRR4302545.3544740/1‑94 (MQ=60)
                      CGCAGCAGCACCAGCGGCTGCGGCGCCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTT                                                                         <  SRR4302545.1137242/94‑1 (MQ=60)
                       GCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTG                                                                        <  SRR4302545.1044410/94‑1 (MQ=60)
                       GCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTG                                                                        <  SRR4302545.7720753/94‑1 (MQ=60)
                       GCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTG                                                                        >  SRR4302545.8915397/1‑94 (MQ=60)
                       GCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTG                                                                        <  SRR4302545.9760943/94‑1 (MQ=60)
                       GCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTG                                                                        <  SRR4302545.1403602/94‑1 (MQ=60)
                       GCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTG                                                                        >  SRR4302545.138157/1‑94 (MQ=60)
                        CAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGG                                                                       >  SRR4302545.3757547/1‑94 (MQ=60)
                        CAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGG                                                                       <  SRR4302545.8569770/94‑1 (MQ=60)
                        CAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGG                                                                       <  SRR4302545.10854619/94‑1 (MQ=60)
                        CAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGG                                                                       >  SRR4302545.4472219/1‑94 (MQ=60)
                         AGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGC                                                                      >  SRR4302545.1319987/1‑94 (MQ=60)
                         AGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGC                                                                      >  SRR4302545.9265220/1‑94 (MQ=60)
                           CAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGA                                                                    >  SRR4302545.3076170/1‑94 (MQ=60)
                            AGCACCAGCGGCTGCGGCGGCAAGAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGAT                                                                   >  SRR4302545.6909762/1‑94 (MQ=60)
                            AGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGGT                                                                   >  SRR4302545.6274471/1‑94 (MQ=60)
                            AGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGAT                                                                   <  SRR4302545.4281067/94‑1 (MQ=60)
                            AGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGAT                                                                   >  SRR4302545.8770103/1‑94 (MQ=60)
                            AGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGAT                                                                   >  SRR4302545.8770102/1‑94 (MQ=60)
                             GCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATA                                                                  <  SRR4302545.6469126/94‑1 (MQ=60)
                             GCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATA                                                                  >  SRR4302545.6882933/1‑94 (MQ=60)
                             GCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATA                                                                  >  SRR4302545.6824917/1‑94 (MQ=60)
                              CACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAA                                                                 >  SRR4302545.6951513/1‑94 (MQ=60)
                              CACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAA                                                                 >  SRR4302545.7306908/1‑94 (MQ=60)
                              CACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAA                                                                 <  SRR4302545.10775600/94‑1 (MQ=60)
                               ACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAA                                                                >  SRR4302545.7016367/1‑94 (MQ=60)
                               ACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAA                                                                >  SRR4302545.10364990/1‑94 (MQ=60)
                                CCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAG                                                               <  SRR4302545.931303/94‑1 (MQ=60)
                                CCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAG                                                               <  SRR4302545.9837652/94‑1 (MQ=60)
                                 CAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGT                                                              <  SRR4302545.9413167/94‑1 (MQ=60)
                                 CAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGT                                                              <  SRR4302545.9413166/94‑1 (MQ=60)
                                 CAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGT                                                              <  SRR4302545.9423997/94‑1 (MQ=60)
                                 CAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGT                                                              <  SRR4302545.6053887/94‑1 (MQ=60)
                                 CAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGT                                                              <  SRR4302545.10115609/94‑1 (MQ=60)
                                  AGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTT                                                             <  SRR4302545.3856227/94‑1 (MQ=60)
                                   GCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTG                                                            <  SRR4302545.6247481/94‑1 (MQ=60)
                                    CGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGA                                                           >  SRR4302545.6009722/1‑94 (MQ=60)
                                     GGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAA                                                          >  SRR4302545.3542811/1‑94 (MQ=60)
                                      GCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAG                                                         <  SRR4302545.8915554/94‑1 (MQ=60)
                                      GCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAG                                                         <  SRR4302545.4312693/94‑1 (MQ=60)
                                      GCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAG                                                         >  SRR4302545.9090645/1‑94 (MQ=60)
                                       CTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGC                                                        >  SRR4302545.9476509/1‑94 (MQ=60)
                                       CTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGC                                                        >  SRR4302545.4103041/1‑94 (MQ=60)
                                       CTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGC                                                        <  SRR4302545.4825479/94‑1 (MQ=60)
                                       CTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGC                                                        >  SRR4302545.2108008/1‑94 (MQ=60)
                                       CTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGC                                                        <  SRR4302545.10601637/94‑1 (MQ=60)
                                       CTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGC                                                        <  SRR4302545.10601638/94‑1 (MQ=60)
                                         GCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTG                                                      <  SRR4302545.1528000/94‑1 (MQ=60)
                                         GCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTG                                                      <  SRR4302545.3584403/94‑1 (MQ=60)
                                         GCGGCGGCAAAAGACGTTAACGTTCCGGATATCAGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTG                                                      <  SRR4302545.8602945/94‑1 (MQ=60)
                                          CGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGA                                                     <  SRR4302545.2648328/94‑1 (MQ=60)
                                           GGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAA                                                    >  SRR4302545.8445208/1‑94 (MQ=60)
                                            GCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAAC                                                   <  SRR4302545.8833526/94‑1 (MQ=60)
                                            GCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAAC                                                   <  SRR4302545.1981267/94‑1 (MQ=60)
                                            GCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAAC                                                   >  SRR4302545.494423/1‑94 (MQ=60)
                                            GCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAAC                                                   <  SRR4302545.5003048/94‑1 (MQ=60)
                                             CGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACA                                                  <  SRR4302545.2764318/94‑1 (MQ=60)
                                             CGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACA                                                  >  SRR4302545.1103943/1‑94 (MQ=60)
                                             CGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACA                                                  >  SRR4302545.1596487/1‑94 (MQ=60)
                                             CGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACA                                                  <  SRR4302545.8034392/94‑1 (MQ=60)
                                              GGCAAAAGCCGTTAACCCTCCGGATATCGGCAGCGACGAAGTTGAAGCGAACGAAATCCAGGTGAAAGGTGGCGCTAAAGTTGAGGCTGAAAAG                                                 >  SRR4302545.5770200/1‑94 (MQ=46)
                                              GGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAG                                                 >  SRR4302545.6304212/1‑94 (MQ=60)
                                              GGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAG                                                 >  SRR4302545.5769904/1‑94 (MQ=60)
                                              GGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAG                                                 <  SRR4302545.4683761/94‑1 (MQ=60)
                                              GGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAG                                                 <  SRR4302545.3891872/94‑1 (MQ=60)
                                               GCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGT                                                >  SRR4302545.10027892/1‑94 (MQ=60)
                                                CAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTC                                               >  SRR4302545.8310470/1‑94 (MQ=60)
                                                CAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTC                                               >  SRR4302545.8667665/1‑94 (MQ=60)
                                                CAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTC                                               <  SRR4302545.4381280/94‑1 (MQ=60)
                                                 AAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCG                                              >  SRR4302545.2494791/1‑94 (MQ=60)
                                                 AAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCG                                              >  SRR4302545.8606919/1‑94 (MQ=60)
                                                 AAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGGCG                                              >  SRR4302545.8248422/1‑94 (MQ=60)
                                                 AAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAATTGGCGATAAAGTTGAAGCTGAACAGTAG                                              >  SRR4302545.8348927/1‑94 (MQ=60)
                                                  AAAGACGTTAACGTTCAGGATATCGGAAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTAGC                                             >  SRR4302545.9236813/1‑94 (MQ=60)
                                                   AAGACGTTACCGTTCCGGATATCGGCAGCGACGCAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGAGATCAAGTTGAAGCTGAACAGTCGCT                                            <  SRR4302545.9687799/94‑1 (MQ=60)
                                                   AAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCT                                            >  SRR4302545.7491855/1‑94 (MQ=60)
                                                    AGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTG                                           <  SRR4302545.8340012/94‑1 (MQ=60)
                                                    AGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTG                                           >  SRR4302545.3476173/1‑94 (MQ=60)
                                                    AGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTG                                           >  SRR4302545.8986409/1‑94 (MQ=60)
                                                    AGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTG                                           <  SRR4302545.4927458/94‑1 (MQ=60)
                                                     GACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGA                                          >  SRR4302545.9362971/1‑94 (MQ=60)
                                                     GACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGA                                          >  SRR4302545.7134444/1‑94 (MQ=60)
                                                      ACGTTAACGTTCCGGATATCGGCAGTGACGAAGTTGAAGCGACCGAAATCCTGGCGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTGGCTGAT                                         >  SRR4302545.1200569/1‑94 (MQ=60)
                                                       CGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATC                                        >  SRR4302545.5173178/1‑94 (MQ=60)
                                                       CGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATC                                        >  SRR4302545.6798968/1‑94 (MQ=60)
                                                       CGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATC                                        >  SRR4302545.6798969/1‑94 (MQ=60)
                                                       CGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATC                                        >  SRR4302545.1963670/1‑94 (MQ=60)
                                                       CGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATC                                        <  SRR4302545.3380605/94‑1 (MQ=60)
                                                        GTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCA                                       >  SRR4302545.7842673/1‑94 (MQ=60)
                                                        GTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCA                                       <  SRR4302545.7904330/94‑1 (MQ=60)
                                                        GTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCA                                       >  SRR4302545.731963/1‑94 (MQ=60)
                                                        GTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCA                                       <  SRR4302545.7128650/94‑1 (MQ=60)
                                                        GTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCA                                       <  SRR4302545.3292083/94‑1 (MQ=60)
                                                          TACCGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACC                                     <  SRR4302545.1877745/94‑1 (MQ=60)
                                                          TAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACC                                     <  SRR4302545.7718934/94‑1 (MQ=60)
                                                           AACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCG                                    <  SRR4302545.10762681/94‑1 (MQ=60)
                                                             CGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTA                                  <  SRR4302545.9542045/94‑1 (MQ=60)
                                                              GTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCCCCGTAG                                 >  SRR4302545.5641603/1‑94 (MQ=60)
                                                              GTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAG                                 >  SRR4302545.3202825/1‑94 (MQ=60)
                                                              GTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAG                                 >  SRR4302545.2564771/1‑94 (MQ=60)
                                                              GTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAG                                 >  SRR4302545.10864157/1‑94 (MQ=60)
                                                              GTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAG                                 <  SRR4302545.10427661/94‑1 (MQ=60)
                                                               TTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGA                                <  SRR4302545.8745107/94‑1 (MQ=60)
                                                                TCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAA                               <  SRR4302545.4079830/94‑1 (MQ=60)
                                                                 CCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAG                              <  SRR4302545.4436349/94‑1 (MQ=60)
                                                                 CCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAG                              >  SRR4302545.8606564/1‑94 (MQ=60)
                                                                 CCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAG                              <  SRR4302545.4777226/94‑1 (MQ=60)
                                                                  CGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGG                             >  SRR4302545.1547005/1‑94 (MQ=60)
                                                                  CGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGG                             >  SRR4302545.4950472/1‑94 (MQ=60)
                                                                     ATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGA                          <  SRR4302545.387625/94‑1 (MQ=60)
                                                                      TATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGAC                         >  SRR4302545.426994/1‑94 (MQ=60)
                                                                       ATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACA                        >  SRR4302545.8919602/1‑94 (MQ=60)
                                                                       ATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACA                        >  SRR4302545.8919601/1‑94 (MQ=60)
                                                                       ATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACA                        >  SRR4302545.2718386/1‑94 (MQ=60)
                                                                        TCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAA                       <  SRR4302545.9549077/94‑1 (MQ=60)
                                                                        TCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAA                       <  SRR4302545.382747/94‑1 (MQ=60)
                                                                        TCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAA                       <  SRR4302545.3578330/94‑1 (MQ=60)
                                                                         CGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACCGTCGCTGATCACCGTAGAAGGCGACAAG                      <  SRR4302545.7600756/94‑1 (MQ=60)
                                                                         CGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAG                      <  SRR4302545.2415596/94‑1 (MQ=60)
                                                                         CGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAG                      <  SRR4302545.101108/94‑1 (MQ=60)
                                                                         CGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAG                      >  SRR4302545.270004/1‑94 (MQ=60)
                                                                          GGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGG                     >  SRR4302545.6562646/1‑94 (MQ=60)
                                                                            CAGCGCCGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCT                   >  SRR4302545.1360520/1‑94 (MQ=60)
                                                                            CAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCT                   >  SRR4302545.1360522/1‑94 (MQ=60)
                                                                             AGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTT                  >  SRR4302545.369070/1‑94 (MQ=60)
                                                                             AGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTT                  <  SRR4302545.10498006/94‑1 (MQ=60)
                                                                              GCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTC                 >  SRR4302545.3809957/1‑94 (MQ=60)
                                                                               CGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCT                <  SRR4302545.8572139/94‑1 (MQ=60)
                                                                               CGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCT                >  SRR4302545.10463034/1‑94 (MQ=60)
                                                                               CGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCT                <  SRR4302545.7138541/94‑1 (MQ=60)
                                                                               CGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCT                >  SRR4302545.8550114/1‑94 (MQ=60)
                                                                               CGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGATGAACAGTCGCTGATCCCCGTAGAAGGCGACAAGGCTTCT                >  SRR4302545.5901643/1‑94 (MQ=60)
                                                                                GACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTA               >  SRR4302545.2805255/1‑94 (MQ=60)
                                                                                 ACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTAT              >  SRR4302545.1316380/1‑94 (MQ=60)
                                                                                 ACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTAT              <  SRR4302545.6688645/94‑1 (MQ=60)
                                                                                  CGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATG             >  SRR4302545.6738701/1‑94 (MQ=60)
                                                                                  CGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATG             <  SRR4302545.8897553/94‑1 (MQ=60)
                                                                                   GAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGG            <  SRR4302545.8057700/94‑1 (MQ=60)
                                                                                   GAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGG            >  SRR4302545.6524027/1‑94 (MQ=60)
                                                                                    AAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGA           <  SRR4302545.6150630/94‑1 (MQ=60)
                                                                                    AAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGA           <  SRR4302545.3291700/94‑1 (MQ=60)
                                                                                     AGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAA          >  SRR4302545.10498802/1‑94 (MQ=60)
                                                                                     AGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAA          <  SRR4302545.10926145/94‑1 (MQ=60)
                                                                                     AGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAA          <  SRR4302545.5558384/94‑1 (MQ=60)
                                                                                      GTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAG         <  SRR4302545.9911826/94‑1 (MQ=60)
                                                                                       TTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGT        <  SRR4302545.10104471/94‑1 (MQ=60)
                                                                                       TTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGT        >  SRR4302545.10607764/1‑94 (MQ=60)
                                                                                       GTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGT        <  SRR4302545.10310317/94‑1 (MQ=60)
                                                                                        TGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGTTTCTATGGAAGTC       >  SRR4302545.4463122/1‑94 (MQ=60)
                                                                                        TGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTT       <  SRR4302545.5796754/94‑1 (MQ=60)
                                                                                          AAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCC     >  SRR4302545.3454926/1‑94 (MQ=60)
                                                                                          AAGCGACCAAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCC     >  SRR4302545.7911850/1‑94 (MQ=60)
                                                                                           AGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCG    <  SRR4302545.10529051/94‑1 (MQ=60)
                                                                                           AGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCG    <  SRR4302545.1837127/94‑1 (MQ=60)
                                                                                           AGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGATCAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCG    >  SRR4302545.4669712/1‑94 (MQ=60)
                                                                                            GCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGG   <  SRR4302545.6914166/94‑1 (MQ=60)
                                                                                             CGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGC  >  SRR4302545.7373300/1‑94 (MQ=60)
                                                                                             CGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGC  >  SRR4302545.3501390/1‑94 (MQ=60)
                                                                                             |                                                                                             
AAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGC  >  NC_000913/125960‑126146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑