Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	126,053	T→C	V120A (GTG→GCG)	aceF →	pyruvate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	126,053	0	T	C	100.0%	839.3 / NA	241	V120A (GTG→GCG)	aceF	pyruvate dehydrogenase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (103/138); total (103/138)

Only 100 of 256 total aligned reads displayed.

AGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTC  >  NC_000913/125963‑126142

agaagaAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGa                                                                                         <  2:4986682/93‑1 (MQ=255)

 gaagaaAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGAc                                                                                        <  2:768563/93‑1 (MQ=255)

 gaagaaAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGAc                                                                                        <  2:5367888/93‑1 (MQ=255)

 gaagaaAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGAc                                                                                        >  1:5070945/1‑93 (MQ=255)

  aagaaagaAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGAcc                                                                                       >  2:3696506/1‑93 (MQ=255)

   agaaagaaGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCg                                                                                      <  2:4244245/93‑1 (MQ=255)

    gaaagaaGCAGCGCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGa                                                                                     <  1:5921540/93‑1 (MQ=255)

      aagaagCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGaaa                                                                                   >  1:887681/1‑93 (MQ=255)

      aagaagCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGaaa                                                                                   <  2:3291779/93‑1 (MQ=255)

         aagCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATcc                                                                                <  2:121247/93‑1 (MQ=255)

          agcagcTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCt                                                                               >  2:1937761/1‑93 (MQ=255)

          agcagcTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCt                                                                               <  2:6463469/93‑1 (MQ=255)

          agcagcTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCt                                                                               <  1:2519479/93‑1 (MQ=255)

          agcagcTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGAGACCGCAATCCt                                                                               >  2:1517206/1‑93 (MQ=255)

           gcagcTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTg                                                                              <  2:2239651/93‑1 (MQ=255)

           gcagcTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTg                                                                              >  1:4599682/1‑93 (MQ=255)

             agcTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTggg                                                                            >  1:1617959/1‑92 (MQ=255)

             agcTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTggg                                                                            >  2:5806968/1‑92 (MQ=255)

              gcTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGGCGAAGTTGAAGCGACCGAAATCCTGGTg                                                                           >  1:3443759/1‑93 (MQ=255)

                tCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGaa                                                                         <  1:54926/93‑1 (MQ=255)

                tCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGGGaa                                                                         >  2:3377255/1‑93 (MQ=255)

                 ccGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGaaa                                                                        <  1:4109673/93‑1 (MQ=255)

                  cGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAg                                                                       <  1:763424/93‑1 (MQ=255)

                   ggCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGt                                                                      >  1:4349316/1‑93 (MQ=255)

                    gcagcagcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGtt                                                                     <  2:686358/93‑1 (MQ=255)

                     cagcagcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTg                                                                    <  2:6207970/93‑1 (MQ=255)

                      agcagcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTgg                                                                   <  1:101553/93‑1 (MQ=255)

                      agcagcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTgg                                                                   >  1:4833089/1‑93 (MQ=255)

                       gcagcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGc                                                                  <  2:4841573/93‑1 (MQ=255)

                       gcagcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGc                                                                  >  1:1126964/1‑93 (MQ=255)

                       gcagcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGc                                                                  >  2:5881374/1‑93 (MQ=255)

                       gcagcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGc                                                                  >  1:544200/1‑93 (MQ=255)

                          gcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGAt                                                               >  1:657214/1‑93 (MQ=255)

                          acaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGAt                                                               <  1:6203695/92‑1 (MQ=255)

                            aCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATaa                                                             >  1:2748328/1‑93 (MQ=255)

                            aCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATaa                                                             <  1:1963450/93‑1 (MQ=255)

                            aCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATaa                                                             <  1:4706881/93‑1 (MQ=255)

                             ccAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATaaa                                                            <  1:978823/93‑1 (MQ=255)

                             ccAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATaaa                                                            <  2:6153996/93‑1 (MQ=255)

                             ccAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATaaa                                                            <  1:2938699/93‑1 (MQ=255)

                             ccAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATaaa                                                            >  1:386912/1‑93 (MQ=255)

                             ccAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATaaa                                                            <  2:606027/93‑1 (MQ=255)

                               aGCGGCTGCGGCGGCAAAAGACTTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGt                                                          <  2:1406474/93‑1 (MQ=255)

                               aGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGt                                                          <  2:3286284/93‑1 (MQ=255)

                                 cGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTg                                                        >  2:1663170/1‑93 (MQ=255)

                                  ggCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGa                                                       <  2:2480330/93‑1 (MQ=255)

                                  ggCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGa                                                       >  2:2751721/1‑93 (MQ=255)

                                   gCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGaa                                                      <  1:4424860/93‑1 (MQ=255)

                                   gCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGaa                                                      >  1:2185788/1‑93 (MQ=255)

                                   gCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGaa                                                      >  2:2850706/1‑93 (MQ=255)

                                    cTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAg                                                     <  2:4689744/93‑1 (MQ=255)

                                     tGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGc                                                    <  1:4446663/93‑1 (MQ=255)

                                      gcggcgGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCt                                                   <  2:1106855/93‑1 (MQ=255)

                                         gcggcAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGaa                                                >  2:1794020/1‑93 (MQ=255)

                                            gcAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGAGCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAg                                             <  1:3747148/93‑1 (MQ=255)

                                            gcAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAg                                             <  2:3099293/93‑1 (MQ=255)

                                             cAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGt                                            <  2:27172/93‑1 (MQ=255)

                                             cAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGt                                            >  1:1927123/1‑93 (MQ=255)

                                             cAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGt                                            <  2:5670589/93‑1 (MQ=255)

                                             cAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGt                                            <  2:5720996/93‑1 (MQ=255)

                                              aaaaGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTc                                           <  1:3141581/93‑1 (MQ=255)

                                              aaaaGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTc                                           <  1:1148754/93‑1 (MQ=255)

                                              aaaaGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTc                                           <  1:5962833/93‑1 (MQ=255)

                                               aaaGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCg                                          >  1:3277329/1‑93 (MQ=255)

                                                 aGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCt                                        >  1:4937954/1‑93 (MQ=255)

                                                 aGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCt                                        >  1:2965790/1‑93 (MQ=255)

                                                      ttAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCa                                   >  1:5212665/1‑93 (MQ=255)

                                                      ttAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCa                                   >  2:3883209/1‑93 (MQ=255)

                                                      ttAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCa                                   >  1:3628122/1‑93 (MQ=255)

                                                         aCGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCg                                >  2:189851/1‑93 (MQ=255)

                                                         aCGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCg                                >  2:4947390/1‑93 (MQ=255)

                                                          cGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGt                               <  1:4222511/93‑1 (MQ=255)

                                                           gTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTa                              <  2:2165017/93‑1 (MQ=255)

                                                            ttCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAg                             >  2:1233985/1‑93 (MQ=255)

                                                            ttCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAg                             >  1:1884588/1‑93 (MQ=255)

                                                             tCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGa                            <  1:2098955/93‑1 (MQ=255)

                                                             tCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGa                            >  2:2439364/1‑93 (MQ=255)

                                                              ccGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGaa                           <  1:697987/93‑1 (MQ=255)

                                                               cGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAg                          >  1:40704/1‑93 (MQ=255)

                                                                 gATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGc                        <  2:2898663/93‑1 (MQ=255)

                                                                 gATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGc                        >  2:390647/1‑93 (MQ=255)

                                                                    atCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGAc                     >  1:2225354/1‑93 (MQ=255)

                                                                     tCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACa                    >  1:2649378/1‑93 (MQ=255)

                                                                     tCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACa                    <  1:1453504/93‑1 (MQ=255)

                                                                     tCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACa                    >  1:1238708/1‑93 (MQ=255)

                                                                      cGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACaa                   <  2:310454/93‑1 (MQ=255)

                                                                        gCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAgg                 >  2:5223367/1‑93 (MQ=255)

                                                                        gCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAgg                 >  2:4196811/1‑93 (MQ=255)

                                                                         cAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGc                <  2:3778948/93‑1 (MQ=255)

                                                                           gCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCtt              >  1:6252074/1‑93 (MQ=255)

                                                                           gCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCtt              <  1:2383819/93‑1 (MQ=255)

                                                                           gCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCtt              >  1:1857919/1‑93 (MQ=255)

                                                                            cgacgaAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTc             <  1:5335221/93‑1 (MQ=255)

                                                                             gacgaAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCt            >  1:2822882/1‑93 (MQ=255)

                                                                              acgaAGTTGAAGCGACCGAAATCCTGGTGAAAGTGGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTa           <  2:5614153/93‑1 (MQ=255)

                                                                                 aaGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATgg        >  2:2651563/1‑93 (MQ=255)

                                                                                 aaGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATgg        <  2:4481793/93‑1 (MQ=255)

                                                                                 aaGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATgg        >  2:6255415/1‑93 (MQ=255)

                                                                                     tGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGt    <  1:563382/93‑1 (MQ=255)

                                                                                       aaGCGACCGAAATCCTGGTGAAAGTTAGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAATGCTTCTATGGAAGTTc  <  1:6077853/93‑1 (MQ=255)

AGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTC  >  NC_000913/125963‑126142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGC  >  NC_000913/125960‑126146

AAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGC                                                                                               <  SRR4302537.5872340/94‑1 (MQ=60)

 AGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCG                                                                                              <  SRR4302537.5307362/94‑1 (MQ=60)

 AGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCG                                                                                              <  SRR4302537.5313798/94‑1 (MQ=60)

 AGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCG                                                                                              <  SRR4302537.3874245/94‑1 (MQ=60)

   AGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGAC                                                                                            >  SRR4302537.5105855/1‑94 (MQ=60)

   AGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGAC                                                                                            <  SRR4302537.3791455/94‑1 (MQ=60)

    GAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACC                                                                                           <  SRR4302537.3281114/94‑1 (MQ=60)

      AGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGA                                                                                         >  SRR4302537.6364300/1‑94 (MQ=60)

      AGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGA                                                                                         <  SRR4302537.4207074/94‑1 (MQ=60)

       GAAAGAAGCAGCGCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAA                                                                                        <  SRR4302537.5962171/94‑1 (MQ=60)

        AAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAA                                                                                       >  SRR4302537.893787/1‑94 (MQ=60)

         AAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAAT                                                                                      <  SRR4302537.293267/94‑1 (MQ=60)

          AGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGGAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATC                                                                                     >  SRR4302537.3912921/1‑94 (MQ=60)

          AGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATC                                                                                     <  SRR4302537.4843015/94‑1 (MQ=60)

          AGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGGTGAAGCGACCGAAATC                                                                                     >  SRR4302537.1137383/1‑94 (MQ=60)

            AAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCT                                                                                   <  SRR4302537.4300121/94‑1 (MQ=60)

             AGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTG                                                                                  >  SRR4302537.4631517/1‑94 (MQ=60)

             AGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTG                                                                                  <  SRR4302537.2537173/94‑1 (MQ=60)

               CAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGG                                                                                >  SRR4302537.1629262/1‑94 (MQ=60)

                AGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGGCGAAGTTGAAGCGACCGAAATCCTGGTG                                                                               >  SRR4302537.3468123/1‑94 (MQ=60)

                AGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTG                                                                               >  SRR4302537.765352/1‑94 (MQ=60)

                AGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTG                                                                               <  SRR4302537.265060/94‑1 (MQ=60)

                  CTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGCAGTTGAAGCGACCGAAATCCTGGTGAA                                                                             >  SRR4302537.2188048/1‑94 (MQ=60)

                   TCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAA                                                                            <  SRR4302537.55332/94‑1 (MQ=60)

                   TCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAA                                                                            >  SRR4302537.5982394/1‑94 (MQ=60)

                    CCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAG                                                                           <  SRR4302537.4138384/94‑1 (MQ=60)

                    CCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAG                                                                           <  SRR4302537.2837778/94‑1 (MQ=60)

                    CCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTCGTGAAAG                                                                           >  SRR4302537.209451/1‑94 (MQ=60)

                     CGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGT                                                                          <  SRR4302537.768646/94‑1 (MQ=60)

                     CGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGT                                                                          >  SRR4302537.4379451/1‑94 (MQ=60)

                     CGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGCTAACGTTCCGGATATCGGCAGCGACGAAATTGAAGCGACCCAAATACTGGTGAAAGT                                                                          >  SRR4302537.5681885/1‑94 (MQ=60)

                        CAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGG                                                                       >  SRR4302537.4866462/1‑94 (MQ=60)

                         AGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGC                                                                      <  SRR4302537.102282/94‑1 (MQ=60)

                         AGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGC                                                                      >  SRR4302537.1134781/1‑94 (MQ=60)

                         AGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGC                                                                      >  SRR4302537.547942/1‑94 (MQ=60)

                          GCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCG                                                                     >  SRR4302537.4475276/1‑94 (MQ=60)

                           CAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGA                                                                    >  SRR4302537.599030/1‑94 (MQ=60)

                            AGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGAT                                                                   >  SRR4302537.661692/1‑94 (MQ=60)

                             GCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATA                                                                  <  SRR4302537.4694976/94‑1 (MQ=60)

                             ACACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATA                                                                  <  SRR4302537.6246455/94‑1 (MQ=60)

                              CACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAA                                                                 >  SRR4302537.2767499/1‑94 (MQ=60)

                               ACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAA                                                                <  SRR4302537.1977102/94‑1 (MQ=60)

                               ACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAA                                                                >  SRR4302537.389586/1‑94 (MQ=60)

                               ACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAA                                                                <  SRR4302537.4739445/94‑1 (MQ=60)

                                CCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTTAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAG                                                               <  SRR4302537.1780418/94‑1 (MQ=60)

                                CCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAG                                                               <  SRR4302537.985580/94‑1 (MQ=60)

                                CCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAG                                                               <  SRR4302537.2959329/94‑1 (MQ=60)

                                  AGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTT                                                             >  SRR4302537.3341969/1‑94 (MQ=60)

                                   GCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTG                                                            <  SRR4302537.2249281/94‑1 (MQ=60)

                                   GCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTG                                                            >  SRR4302537.934930/1‑94 (MQ=60)

                                    CGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGA                                                           >  SRR4302537.1069377/1‑94 (MQ=60)

                                     GGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAA                                                          <  SRR4302537.4384550/94‑1 (MQ=60)

                                     GGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAA                                                          >  SRR4302537.2201187/1‑94 (MQ=60)

                                     GGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAA                                                          <  SRR4302537.3317673/94‑1 (MQ=60)

                                     GGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAA                                                          <  SRR4302537.1947277/94‑1 (MQ=60)

                                     GGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAA                                                          <  SRR4302537.5362201/94‑1 (MQ=60)

                                     GGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAA                                                          <  SRR4302537.6411017/94‑1 (MQ=60)

                                      GCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAG                                                         <  SRR4302537.4455421/94‑1 (MQ=60)

                                      GCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAG                                                         <  SRR4302537.583956/94‑1 (MQ=60)

                                       CTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGC                                                        <  SRR4302537.218956/94‑1 (MQ=60)

                                       CTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGC                                                        >  SRR4302537.1900023/1‑94 (MQ=60)

                                       CTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGGTGAAGC                                                        >  SRR4302537.1876016/1‑94 (MQ=60)

                                        TGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCT                                                       <  SRR4302537.4477372/94‑1 (MQ=60)

                                         GCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTG                                                      <  SRR4302537.3685576/94‑1 (MQ=60)

                                           GGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAA                                                    >  SRR4302537.1453565/1‑94 (MQ=60)

                                            GCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAAC                                                   >  SRR4302537.5342118/1‑94 (MQ=60)

                                              GGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAG                                                 <  SRR4302537.661820/94‑1 (MQ=60)

                                               GCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGAGCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGT                                                <  SRR4302537.3773467/94‑1 (MQ=60)

                                               GCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGT                                                >  SRR4302537.1940497/1‑94 (MQ=60)

                                                CAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTC                                               >  SRR4302537.3088413/1‑94 (MQ=60)

                                                 AAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCG                                              <  SRR4302537.6003760/94‑1 (MQ=60)

                                                 AAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCG                                              >  SRR4302537.3570785/1‑94 (MQ=60)

                                                 AAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCG                                              >  SRR4302537.2964624/1‑94 (MQ=60)

                                                 AAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCG                                              <  SRR4302537.1156741/94‑1 (MQ=60)

                                                 AAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCG                                              >  SRR4302537.3300459/1‑94 (MQ=60)

                                                 AAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCG                                              <  SRR4302537.3163627/94‑1 (MQ=60)

                                                 AAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGGTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAAAAGTCG                                              >  SRR4302537.1144570/1‑94 (MQ=60)

                                                  AAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGC                                             <  SRR4302537.311186/94‑1 (MQ=60)

                                                   AAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCT                                            >  SRR4302537.6254408/1‑94 (MQ=60)

                                                   AAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCT                                            <  SRR4302537.3840365/94‑1 (MQ=60)

                                                   AAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCT                                            >  SRR4302537.551933/1‑94 (MQ=60)

                                                   AAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCT                                            <  SRR4302537.2822116/94‑1 (MQ=60)

                                                   AAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCT                                            >  SRR4302537.4972012/1‑94 (MQ=60)

                                                   AAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCT                                            >  SRR4302537.2986596/1‑94 (MQ=60)

                                                     GACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGA                                          <  SRR4302537.1463604/94‑1 (MQ=60)

                                                      ACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGAT                                         >  SRR4302537.197044/1‑94 (MQ=60)

                                                       CGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCTAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATC                                        >  SRR4302537.4580082/1‑94 (MQ=60)

                                                       CGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATC                                        <  SRR4302537.6213280/94‑1 (MQ=60)

                                                       CGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATC                                        <  SRR4302537.6006899/94‑1 (MQ=60)

                                                        GTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCA                                       >  SRR4302537.3653693/1‑94 (MQ=60)

                                                        GTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCA                                       >  SRR4302537.3772114/1‑94 (MQ=60)

                                                        GTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCA                                       >  SRR4302537.5248506/1‑94 (MQ=60)

                                                        GTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCA                                       >  SRR4302537.4472618/1‑94 (MQ=60)

                                                          TAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACC                                     <  SRR4302537.2919116/94‑1 (MQ=60)

                                                            ACGTTCCGGATATCGGCAACGGCGAAGTTGAAGCGGCCGAAATCCTGGTGAAAGTTGGGGATAAAGGTGAAGCTGAACAGTCGCTGATCACCGT                                   <  SRR4302537.3699730/94‑1 (MQ=60)

                                                             CGTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTA                                  <  SRR4302537.4251924/94‑1 (MQ=60)

                                                              GTTCCGGATATCGGCATCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAG                                 >  SRR4302537.193407/1‑94 (MQ=60)

                                                              GTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAG                                 >  SRR4302537.1897695/1‑94 (MQ=60)

                                                              GTTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAG                                 <  SRR4302537.577594/94‑1 (MQ=60)

                                                               TTCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGA                                <  SRR4302537.2606533/94‑1 (MQ=60)

                                                                TCCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAA                               <  SRR4302537.2113755/94‑1 (MQ=60)

                                                                 CCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAG                              <  SRR4302537.1529312/94‑1 (MQ=60)

                                                                 CCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAG                              >  SRR4302537.41010/1‑94 (MQ=60)

                                                                 CCGGATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAG                              <  SRR4302537.702768/94‑1 (MQ=60)

                                                                    GATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCG                           <  SRR4302537.893448/94‑1 (MQ=60)

                                                                     ATATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGA                          >  SRR4302537.2440481/1‑94 (MQ=60)

                                                                      TATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGAC                         >  SRR4302537.2241028/1‑94 (MQ=60)

                                                                       ATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACA                        >  SRR4302537.1247323/1‑94 (MQ=60)

                                                                       ATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACA                        >  SRR4302537.2667906/1‑94 (MQ=60)

                                                                       ATCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACA                        <  SRR4302537.5074902/94‑1 (MQ=60)

                                                                        TCGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAA                       <  SRR4302537.1463637/94‑1 (MQ=60)

                                                                         CGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAG                      <  SRR4302537.5086186/94‑1 (MQ=60)

                                                                         CGGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAG                      <  SRR4302537.969836/94‑1 (MQ=60)

                                                                          GGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGG                     <  SRR4302537.1211840/94‑1 (MQ=60)

                                                                          GGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGG                     >  SRR4302537.4535119/1‑94 (MQ=60)

                                                                          GGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGG                     <  SRR4302537.4953086/94‑1 (MQ=60)

                                                                          GGCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGG                     >  SRR4302537.2300243/1‑94 (MQ=60)

                                                                           GCAGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGC                    >  SRR4302537.1729918/1‑94 (MQ=60)

                                                                             AGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTT                  >  SRR4302537.1870858/1‑94 (MQ=60)

                                                                             AGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTT                  >  SRR4302537.871930/1‑94 (MQ=60)

                                                                             AGCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTT                  >  SRR4302537.6295024/1‑94 (MQ=60)

                                                                              GCGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTC                 <  SRR4302537.2400611/94‑1 (MQ=60)

                                                                               CGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCT                <  SRR4302537.4130247/94‑1 (MQ=60)

                                                                               CGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCT                <  SRR4302537.5371802/94‑1 (MQ=60)

                                                                               CGACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCT                >  SRR4302537.2842611/1‑94 (MQ=60)

                                                                                GACGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTA               >  SRR4302537.5459725/1‑94 (MQ=60)

                                                                                  CGAAGTTGAAGCGACCGAAATCGTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATG             <  SRR4302537.2228392/94‑1 (MQ=60)

                                                                                  CGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATG             >  SRR4302537.6229435/1‑94 (MQ=60)

                                                                                  CGAAGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATG             <  SRR4302537.4678530/94‑1 (MQ=60)

                                                                                     AGTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAA          >  SRR4302537.5644921/1‑94 (MQ=60)

                                                                                      GTTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAG         >  SRR4302537.2767316/1‑94 (MQ=60)

                                                                                       TTGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGT        >  SRR4302537.5490702/1‑94 (MQ=60)

                                                                                        TGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTT       <  SRR4302537.716216/94‑1 (MQ=60)

                                                                                        TGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTT       <  SRR4302537.567257/94‑1 (MQ=60)

                                                                                        TGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTT       <  SRR4302537.4110081/94‑1 (MQ=60)

                                                                                        TGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTT       <  SRR4302537.4812319/94‑1 (MQ=60)

                                                                                        TGAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTT       >  SRR4302537.4282264/1‑94 (MQ=60)

                                                                                         GAAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTC      <  SRR4302537.5135488/94‑1 (MQ=60)

                                                                                          AAGCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCC     >  SRR4302537.170786/1‑94 (MQ=60)

                                                                                          AAGCGACCGAAATCCTGGTGAAAGTTAGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAATGCTTCTATGGAAGTTCC     <  SRR4302537.6119619/94‑1 (MQ=60)

                                                                                            GCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGG   >  SRR4302537.3489278/1‑94 (MQ=60)

                                                                                            GCGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGG   <  SRR4302537.4127603/94‑1 (MQ=60)

                                                                                             CGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGC  >  SRR4302537.338934/1‑94 (MQ=60)

                                                                                             CGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGC  >  SRR4302537.5216959/1‑94 (MQ=60)

AAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGC  >  NC_000913/125960‑126146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`