Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 144,667 C→T R31W (CGG→TGG)  yadI → putative PTS enzyme IIA component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913144,6670CT98.9% 1347.2 / ‑1.3 355R31W (CGG→TGG) yadIputative PTS enzyme IIA component
Reads supporting (aligned to +/- strand):  ref base C (1/3);  new base T (159/192);  total (160/195)
Fisher's exact test for biased strand distribution p-value = 6.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01

Only 100 of 365 total aligned reads displayed.

GATGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCCGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTG  >  NC_000913/144576‑144757
                                                                                           |                                                                                          
gaggTTAGGTTGGGTAATTACCTGTCACGATGGCCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTgg                                                                                          <  1:7703773/91‑1 (MQ=255)
gATGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTgg                                                                                          <  2:5932648/94‑1 (MQ=255)
 aTGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTggg                                                                                         <  1:10380839/94‑1 (MQ=255)
 aTGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTggg                                                                                         >  2:5714415/1‑94 (MQ=255)
 aTGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTggg                                                                                         >  2:9240366/1‑94 (MQ=255)
  tGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGc                                                                                        >  1:5934954/1‑94 (MQ=255)
  tGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGc                                                                                        >  2:914513/1‑94 (MQ=255)
    ttAGGTTGGGGAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCg                                                                                      <  2:1513738/94‑1 (MQ=255)
         ttGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAAt                                                                                 <  1:1694658/94‑1 (MQ=255)
          tGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCCGGGCCGTGAAtt                                                                                >  2:9639034/1‑94 (MQ=255)
           gggTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAAttt                                                                               >  1:2812202/1‑94 (MQ=255)
           gggTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAAttt                                                                               >  2:2853761/1‑94 (MQ=255)
            ggTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTc                                                                              >  1:2126092/1‑94 (MQ=255)
            ggTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTc                                                                              <  2:4229972/94‑1 (MQ=255)
             gTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCTCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCt                                                                             <  2:10454463/94‑1 (MQ=255)
              tAATTCCCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTg                                                                            <  1:129608/94‑1 (MQ=255)
               aaTTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTgg                                                                           <  2:4663086/94‑1 (MQ=255)
               aaTTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTgg                                                                           >  1:8054565/1‑94 (MQ=255)
                 ttACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGgcg                                                                         <  1:2247628/94‑1 (MQ=255)
                  tACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGgcgc                                                                        >  1:1447197/1‑94 (MQ=255)
                  tACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGgcgc                                                                        >  1:3593345/1‑94 (MQ=255)
                   aCCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGcgcg                                                                       <  2:8269980/94‑1 (MQ=255)
                   aCCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGcgcg                                                                       <  1:10226453/94‑1 (MQ=255)
                   aCCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGcgcg                                                                       >  1:9033316/1‑94 (MQ=255)
                     cTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGa                                                                     >  1:2898876/1‑94 (MQ=255)
                      tGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGAt                                                                    <  1:5863371/94‑1 (MQ=255)
                      tGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGAt                                                                    >  1:5531948/1‑94 (MQ=255)
                       gTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGAtt                                                                   <  2:2660884/94‑1 (MQ=255)
                         cACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTaa                                                                 <  1:1859955/94‑1 (MQ=255)
                          aCGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAg                                                                <  1:8860069/94‑1 (MQ=255)
                           cGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGc                                                               >  1:6295157/1‑94 (MQ=255)
                             aTGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGctc                                                             >  1:5573457/1‑94 (MQ=255)
                             aTGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGctc                                                             >  1:7529950/1‑94 (MQ=255)
                              tGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGctct                                                            >  2:88306/1‑94 (MQ=255)
                               gACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTa                                                           <  2:4424960/94‑1 (MQ=255)
                               gACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTa                                                           <  1:1182289/94‑1 (MQ=255)
                                 ccGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAat                                                         <  1:450865/94‑1 (MQ=255)
                                 ccGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAat                                                         >  2:7055816/1‑94 (MQ=255)
                                 ccGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAat                                                         >  1:8449139/1‑94 (MQ=255)
                                  cGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAata                                                        <  1:4033401/94‑1 (MQ=255)
                                  cGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAata                                                        <  2:3160920/94‑1 (MQ=255)
                                   gggCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAatat                                                       <  1:4174547/94‑1 (MQ=255)
                                   gggCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAatat                                                       >  1:5703225/1‑94 (MQ=255)
                                   gggCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAatat                                                       <  2:7612097/94‑1 (MQ=255)
                                    ggCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATg                                                      <  2:4975722/94‑1 (MQ=255)
                                       gcAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTc                                                   >  2:8876044/1‑94 (MQ=255)
                                       gcAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTc                                                   >  2:6582833/1‑94 (MQ=255)
                                       gcAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTc                                                   <  1:2700528/94‑1 (MQ=255)
                                        cAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCa                                                  >  1:3128702/1‑94 (MQ=255)
                                        cAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCa                                                  <  1:1251335/94‑1 (MQ=255)
                                            agaTACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCg                                              >  2:678190/1‑94 (MQ=255)
                                             gaTACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATACTCAGCCGc                                             >  2:5121821/1‑94 (MQ=255)
                                              aTACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGct                                            >  1:2974972/1‑93 (MQ=255)
                                              aTACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCa                                            <  1:9869281/94‑1 (MQ=255)
                                                aCTGGATGCGCTGGAGAAAAAACATGGGGCAGTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCatg                                          <  2:4482126/94‑1 (MQ=255)
                                                aCTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCatg                                          >  1:9537519/1‑94 (MQ=255)
                                                  tGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCatgat                                        <  1:2752723/94‑1 (MQ=255)
                                                   ggATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCatgatg                                       >  1:5242222/1‑94 (MQ=255)
                                                    gATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGAtgt                                      <  2:1039035/94‑1 (MQ=255)
                                                    gATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGAtgt                                      <  1:915633/94‑1 (MQ=255)
                                                     aTGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGAtgtg                                     >  1:7743049/1‑94 (MQ=255)
                                                      tGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGc                                    >  1:9311959/1‑94 (MQ=255)
                                                      tGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGc                                    <  2:3957964/94‑1 (MQ=255)
                                                        cgcTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGa                                  >  1:8557880/1‑94 (MQ=255)
                                                        cgcTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGa                                  <  2:1900217/94‑1 (MQ=255)
                                                        cgcTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGa                                  >  1:3928852/1‑94 (MQ=255)
                                                         gcTGGAGAAAAAACATGGGGCAGTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTTCGAt                                 <  2:9892533/94‑1 (MQ=255)
                                                         gcTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGAt                                 <  2:6442893/94‑1 (MQ=255)
                                                         gcTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGAt                                 <  2:1135325/94‑1 (MQ=255)
                                                          cTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATg                                >  2:2212824/1‑94 (MQ=255)
                                                           tgaagaAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGc                               <  1:10583133/91‑1 (MQ=255)
                                                           tGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGc                               <  2:8635498/94‑1 (MQ=255)
                                                            ggAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGct                              <  1:7881027/94‑1 (MQ=255)
                                                            ggAGAAAAAACATGGGGCACTTCTTCAGTGATGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGct                              <  2:5286518/94‑1 (MQ=255)
                                                             gagaAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGctc                             <  2:5648825/94‑1 (MQ=255)
                                                              ggaAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGctct                            <  2:8053254/93‑1 (MQ=255)
                                                              agaAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGctct                            <  2:7502650/94‑1 (MQ=255)
                                                              agaAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGctct                            >  1:3056476/1‑94 (MQ=255)
                                                               gaAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTg                           <  2:3628608/94‑1 (MQ=255)
                                                                 aaaaaCATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCa                         <  1:666544/94‑1 (MQ=255)
                                                                  aaaaCATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCAt                        >  1:685821/1‑94 (MQ=255)
                                                                    aaCATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGa                      >  1:7112811/1‑94 (MQ=255)
                                                                      cATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAg                    >  2:232935/1‑94 (MQ=255)
                                                                       aTGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGc                   >  1:1990612/1‑94 (MQ=255)
                                                                          gggCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGa                <  1:10030891/94‑1 (MQ=255)
                                                                            gCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGAct              <  1:9276126/94‑1 (MQ=255)
                                                                            gCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGAct              >  1:3457533/1‑94 (MQ=255)
                                                                              aCTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGActct            >  2:3005134/1‑94 (MQ=255)
                                                                              aCTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGActct            <  1:6959427/94‑1 (MQ=255)
                                                                              aCTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGActct            >  2:1290457/1‑94 (MQ=255)
                                                                               cttcttCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTg           >  2:6184562/1‑94 (MQ=255)
                                                                               cttcttCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTg           <  1:6466584/94‑1 (MQ=255)
                                                                               cttctgCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTg           <  2:3930588/94‑1 (MQ=255)
                                                                                   ttcAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTgg       >  2:1598861/1‑93 (MQ=255)
                                                                                    tcATTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAg      <  1:4604881/94‑1 (MQ=255)
                                                                                    tcAGTGCTGGGCCGTGTCTTTCTGGCGCGGATTACGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAg      <  1:9680245/94‑1 (MQ=255)
                                                                                     cAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAgg     >  2:7951670/1‑94 (MQ=255)
                                                                                      aGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAggg    >  2:10177830/1‑94 (MQ=255)
                                                                                       gTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGgt   <  2:2470700/94‑1 (MQ=255)
                                                                                        tGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAgggag  >  1:8191781/1‑92 (MQ=255)
                                                                                           |                                                                                          
GATGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCCGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTG  >  NC_000913/144576‑144757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 217 total aligned reads displayed.
GCGATGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCCGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCA  >  NC_000913/144574‑144760
                                                                                             |                                                                                             
GCGATGTTATGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCCCTTCTTCAGTGCT                                                                                               <  SRR4302545.10838288/94‑1 (MQ=60)
GCGATGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCT                                                                                               <  SRR4302545.6860598/94‑1 (MQ=60)
GCGATGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCT                                                                                               <  SRR4302545.9477789/94‑1 (MQ=60)
GCGATGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCT                                                                                               <  SRR4302545.2593918/94‑1 (MQ=60)
GCGATGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCT                                                                                               >  SRR4302545.6063050/1‑94 (MQ=60)
 TGATGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTG                                                                                              >  SRR4302545.8717828/1‑94 (MQ=60)
 CGATGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTG                                                                                              >  SRR4302545.4981063/1‑94 (MQ=60)
 CGATGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTG                                                                                              <  SRR4302545.4904488/94‑1 (MQ=60)
 CGATGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTG                                                                                              <  SRR4302545.270639/94‑1 (MQ=60)
 CGATGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTG                                                                                              >  SRR4302545.2756243/1‑94 (MQ=60)
  GATGTTAGGTTGGGTAATTACCTGTCGCGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGG                                                                                             <  SRR4302545.1050817/94‑1 (MQ=60)
  GATGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGG                                                                                             <  SRR4302545.7921868/94‑1 (MQ=60)
  GATGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGG                                                                                             <  SRR4302545.2255027/94‑1 (MQ=60)
  GATGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGG                                                                                             <  SRR4302545.208111/94‑1 (MQ=60)
  GATGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGG                                                                                             >  SRR4302545.2886315/1‑94 (MQ=60)
  GATGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGG                                                                                             >  SRR4302545.8927040/1‑94 (MQ=60)
   ATGTTAGGTTGGGTAATTACCTGTCACGATGCCCGGTCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCCGGG                                                                                            <  SRR4302545.7615076/94‑1 (MQ=60)
   ATGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGG                                                                                            >  SRR4302545.1729944/1‑94 (MQ=60)
   ATGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGG                                                                                            <  SRR4302545.10686913/94‑1 (MQ=60)
   ATGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGG                                                                                            >  SRR4302545.3186051/1‑94 (MQ=60)
   ATGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGG                                                                                            <  SRR4302545.9821552/94‑1 (MQ=60)
    TGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGC                                                                                           >  SRR4302545.7929464/1‑94 (MQ=60)
    TGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGC                                                                                           >  SRR4302545.6095089/1‑94 (MQ=60)
    TGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGC                                                                                           <  SRR4302545.1819689/94‑1 (MQ=60)
    TGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGC                                                                                           >  SRR4302545.10034458/1‑94 (MQ=60)
     GTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCC                                                                                          <  SRR4302545.7059426/94‑1 (MQ=60)
     GTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCC                                                                                          >  SRR4302545.8175543/1‑94 (MQ=60)
       TAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGT                                                                                        >  SRR4302545.9019522/1‑94 (MQ=60)
        AGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTG                                                                                       <  SRR4302545.7793062/94‑1 (MQ=60)
         GGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGA                                                                                      >  SRR4302545.1731477/1‑94 (MQ=60)
           TTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAAT                                                                                    >  SRR4302545.9152765/1‑94 (MQ=60)
           TTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAAT                                                                                    <  SRR4302545.1734917/94‑1 (MQ=60)
            TGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATT                                                                                   >  SRR4302545.3674430/1‑94 (MQ=60)
            TGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATT                                                                                   >  SRR4302545.9318412/1‑94 (MQ=60)
            TGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGGGAAAT                                                                                   >  SRR4302545.9318126/1‑94 (MQ=60)
             GGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTT                                                                                  >  SRR4302545.3926945/1‑94 (MQ=60)
             GGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTT                                                                                  >  SRR4302545.2880269/1‑94 (MQ=60)
              GGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTC                                                                                 >  SRR4302545.2178266/1‑94 (MQ=60)
                TAATTCCCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTG                                                                               <  SRR4302545.132514/94‑1 (MQ=60)
                TAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTG                                                                               >  SRR4302545.6609038/1‑94 (MQ=60)
                TAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTG                                                                               >  SRR4302545.6609035/1‑94 (MQ=60)
                 AATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGG                                                                              <  SRR4302545.4100981/94‑1 (MQ=60)
                 AATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGG                                                                              >  SRR4302545.8284711/1‑94 (MQ=60)
                 AATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGG                                                                              <  SRR4302545.4100979/94‑1 (MQ=60)
                 AATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGG                                                                              >  SRR4302545.9798562/1‑94 (MQ=60)
                  ATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGC                                                                             >  SRR4302545.2080065/1‑94 (MQ=60)
                   TTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCG                                                                            <  SRR4302545.5770835/94‑1 (MQ=60)
                   TTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCG                                                                            <  SRR4302545.2302375/94‑1 (MQ=60)
                   TTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCG                                                                            <  SRR4302545.3094256/94‑1 (MQ=60)
                    TACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGC                                                                           >  SRR4302545.1480236/1‑94 (MQ=60)
                    TACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGC                                                                           >  SRR4302545.8149013/1‑94 (MQ=60)
                    TACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGC                                                                           >  SRR4302545.3681014/1‑94 (MQ=60)
                    TACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGC                                                                           >  SRR4302545.7342929/1‑94 (MQ=60)
                    TACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGC                                                                           >  SRR4302545.7342930/1‑94 (MQ=60)
                     ACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCG                                                                          >  SRR4302545.7749244/1‑94 (MQ=60)
                     ACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCG                                                                          >  SRR4302545.9296581/1‑94 (MQ=60)
                     ACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCG                                                                          <  SRR4302545.10528521/94‑1 (MQ=60)
                     ACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCG                                                                          <  SRR4302545.10528523/94‑1 (MQ=60)
                      CCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGG                                                                         >  SRR4302545.2932782/1‑94 (MQ=60)
                      CCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGG                                                                         <  SRR4302545.8774308/94‑1 (MQ=60)
                       CTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGA                                                                        >  SRR4302545.2969134/1‑94 (MQ=60)
                       CTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGA                                                                        <  SRR4302545.3172537/94‑1 (MQ=60)
                        TGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGAT                                                                       <  SRR4302545.6021338/94‑1 (MQ=60)
                        TGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGAT                                                                       >  SRR4302545.5736186/1‑94 (MQ=60)
                        TGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGAT                                                                       <  SRR4302545.2284861/94‑1 (MQ=60)
                        TGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGAT                                                                       >  SRR4302545.5677999/1‑94 (MQ=60)
                        TGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGAT                                                                       <  SRR4302545.6021340/94‑1 (MQ=60)
                        TGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGAT                                                                       >  SRR4302545.5677998/1‑94 (MQ=60)
                           CACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAA                                                                    >  SRR4302545.3659040/1‑94 (MQ=60)
                            ACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAG                                                                   <  SRR4302545.9460184/94‑1 (MQ=60)
                            ACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAG                                                                   <  SRR4302545.9117444/94‑1 (MQ=60)
                             CGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGC                                                                  >  SRR4302545.2021944/1‑94 (MQ=60)
                             CGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGC                                                                  >  SRR4302545.6466809/1‑94 (MQ=60)
                             CGATGACCGGACGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTACTTCAGTGCTGGGCCGCGAATTTCTGGCGCGCATTAAGC                                                                  >  SRR4302545.3270110/1‑94 (MQ=60)
                               ATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTC                                                                >  SRR4302545.7742960/1‑94 (MQ=60)
                               ATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTC                                                                >  SRR4302545.5721014/1‑94 (MQ=60)
                                AGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCT                                                               <  SRR4302545.8376450/94‑1 (MQ=60)
                                 GACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTA                                                              <  SRR4302545.1208570/94‑1 (MQ=60)
                                 GACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTA                                                              <  SRR4302545.2796032/94‑1 (MQ=60)
                                 GACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTA                                                              >  SRR4302545.4262576/1‑94 (MQ=60)
                                  ACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAA                                                             <  SRR4302545.4919324/94‑1 (MQ=60)
                                  ACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACATCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAA                                                             >  SRR4302545.1293016/1‑94 (MQ=60)
                                   CCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAAT                                                            <  SRR4302545.460855/94‑1 (MQ=60)
                                   CCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAAT                                                            >  SRR4302545.8693404/1‑94 (MQ=60)
                                    CGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATA                                                           <  SRR4302545.4134363/94‑1 (MQ=60)
                                     GGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTTGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATAT                                                          <  SRR4302545.10687887/94‑1 (MQ=60)
                                     GGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATAT                                                          <  SRR4302545.4278734/94‑1 (MQ=60)
                                     GGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATAT                                                          <  SRR4302545.5023401/94‑1 (MQ=60)
                                     GGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATAT                                                          <  SRR4302545.2784554/94‑1 (MQ=60)
                                     GGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATAT                                                          <  SRR4302545.300583/94‑1 (MQ=60)
                                     GGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATAT                                                          <  SRR4302545.300584/94‑1 (MQ=60)
                                     GGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATAT                                                          >  SRR4302545.5855984/1‑94 (MQ=60)
                                     GGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATAT                                                          >  SRR4302545.7192252/1‑94 (MQ=60)
                                      GGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATG                                                         >  SRR4302545.1961858/1‑94 (MQ=60)
                                        CGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCT                                                       >  SRR4302545.6390270/1‑94 (MQ=60)
                                         GCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTC                                                      >  SRR4302545.6023803/1‑94 (MQ=60)
                                         GCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTC                                                      <  SRR4302545.2765573/94‑1 (MQ=60)
                                         GCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTC                                                      >  SRR4302545.9560212/1‑94 (MQ=60)
                                          CAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCA                                                     >  SRR4302545.3205864/1‑94 (MQ=60)
                                          CAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCA                                                     <  SRR4302545.1393740/94‑1 (MQ=60)
                                          CAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCA                                                     >  SRR4302545.5881124/1‑94 (MQ=60)
                                          CAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCA                                                     <  SRR4302545.7140804/94‑1 (MQ=60)
                                             GAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCC                                                  >  SRR4302545.3421036/1‑94 (MQ=60)
                                              AGATACTGGATGCGCTGGAGAAAAGACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCG                                                 <  SRR4302545.3029651/94‑1 (MQ=60)
                                              AGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCG                                                 <  SRR4302545.2342479/94‑1 (MQ=60)
                                              AGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCG                                                 >  SRR4302545.1163353/1‑94 (MQ=60)
                                              AGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCG                                                 <  SRR4302545.1237054/94‑1 (MQ=60)
                                               GATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGC                                                <  SRR4302545.6888169/94‑1 (MQ=60)
                                               GATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGC                                                >  SRR4302545.4205687/1‑94 (MQ=60)
                                               GATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGC                                                <  SRR4302545.6888167/94‑1 (MQ=60)
                                               GATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGC                                                <  SRR4302545.8812604/94‑1 (MQ=60)
                                                ATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCT                                               >  SRR4302545.3047461/1‑94 (MQ=60)
                                                ATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCA                                               <  SRR4302545.10162569/94‑1 (MQ=60)
                                                 TACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCAT                                              >  SRR4302545.5715434/1‑94 (MQ=60)
                                                  ACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATG                                             >  SRR4302545.4293327/1‑94 (MQ=60)
                                                  ACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATG                                             <  SRR4302545.10533126/94‑1 (MQ=60)
                                                  ACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATG                                             >  SRR4302545.9818387/1‑94 (MQ=60)
                                                  ACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATG                                             >  SRR4302545.6281114/1‑94 (MQ=60)
                                                   CTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGA                                            >  SRR4302545.10817793/1‑94 (MQ=60)
                                                    TGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGAT                                           <  SRR4302545.2819134/94‑1 (MQ=60)
                                                     GGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATG                                          >  SRR4302545.5377528/1‑94 (MQ=60)
                                                     GGATGCGCTAGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATG                                          <  SRR4302545.831603/94‑1 (MQ=60)
                                                      GATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGT                                         <  SRR4302545.936172/94‑1 (MQ=60)
                                                      GATGAGCTGGAGAAAAAACGTGGGGCCCTTCTTCAGCGCTGGGCCGTGAACTTCTGGCGCGGATTAAGCTCTAAGATGCTCAGCCGCATGATGT                                         <  SRR4302545.2044639/94‑1 (MQ=60)
                                                       ATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTG                                        <  SRR4302545.6016297/94‑1 (MQ=60)
                                                       ATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTG                                        >  SRR4302545.7962179/1‑94 (MQ=60)
                                                       ATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCCCATGATGTG                                        <  SRR4302545.3737610/94‑1 (MQ=60)
                                                       ATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGAGCGGATTAAGCTCTAATATGCTCAACCGCATGATGTG                                        >  SRR4302545.7962180/1‑94 (MQ=60)
                                                        TGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGC                                       >  SRR4302545.9584972/1‑94 (MQ=60)
                                                        TGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGC                                       >  SRR4302545.9584971/1‑94 (MQ=60)
                                                          CGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTACGCTCTAATATGCTCAGCCGCATGATGTGCGA                                     >  SRR4302545.4276972/1‑94 (MQ=60)
                                                          CGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGA                                     >  SRR4302545.8805304/1‑94 (MQ=60)
                                                          CGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGA                                     >  SRR4302545.9964011/1‑94 (MQ=60)
                                                          CGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGA                                     >  SRR4302545.4026948/1‑94 (MQ=60)
                                                            CTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATG                                   >  SRR4302545.667761/1‑94 (MQ=60)
                                                            CTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATG                                   <  SRR4302545.513448/94‑1 (MQ=60)
                                                             TGAAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGC                                  <  SRR4302545.10894852/94‑1 (MQ=60)
                                                              GGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCT                                 <  SRR4302545.8104648/94‑1 (MQ=60)
                                                              GGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCT                                 <  SRR4302545.8104646/94‑1 (MQ=60)
                                                                AGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCT                               >  SRR4302545.3131421/1‑94 (MQ=60)
                                                                AGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCT                               <  SRR4302545.1397508/94‑1 (MQ=60)
                                                                 GAAAAAACATGGGGCCCTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTG                              <  SRR4302545.9879826/94‑1 (MQ=60)
                                                                 GAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGCTTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTG                              >  SRR4302545.8445450/1‑94 (MQ=60)
                                                                 GAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTG                              <  SRR4302545.4387137/94‑1 (MQ=60)
                                                                 GAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTG                              <  SRR4302545.1200773/94‑1 (MQ=60)
                                                                 GAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTG                              >  SRR4302545.1314158/1‑94 (MQ=60)
                                                                  AAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTACGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGC                             >  SRR4302545.568153/1‑94 (MQ=60)
                                                                   AAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCA                            <  SRR4302545.1221588/94‑1 (MQ=60)
                                                                   AAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCA                            <  SRR4302545.1221584/94‑1 (MQ=60)
                                                                   AAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCA                            <  SRR4302545.681395/94‑1 (MQ=60)
                                                                    AAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCAT                           >  SRR4302545.10138838/1‑94 (MQ=60)
                                                                    AAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCAT                           >  SRR4302545.701143/1‑94 (MQ=60)
                                                                     AAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATG                          >  SRR4302545.5842824/1‑94 (MQ=60)
                                                                      AACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGA                         >  SRR4302545.7744957/1‑94 (MQ=60)
                                                                      AACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGA                         >  SRR4302545.7309908/1‑94 (MQ=60)
                                                                       ACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAAGATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAA                        <  SRR4302545.3074965/94‑1 (MQ=60)
                                                                         ATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGC                      >  SRR4302545.2039860/1‑94 (MQ=60)
                                                                         ATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGC                      <  SRR4302545.2362066/94‑1 (MQ=60)
                                                                          TGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCG                     <  SRR4302545.9467956/94‑1 (MQ=60)
                                                                           GGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGG                    <  SRR4302545.5511002/94‑1 (MQ=60)
                                                                           GGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGG                    >  SRR4302545.9118348/1‑94 (MQ=60)
                                                                           GGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGG                    <  SRR4302545.8617073/94‑1 (MQ=60)
                                                                            GGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGA                   <  SRR4302545.10091208/94‑1 (MQ=60)
                                                                            GGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGA                   <  SRR4302545.10328086/94‑1 (MQ=60)
                                                                              GCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACT                 >  SRR4302545.3542169/1‑94 (MQ=60)
                                                                              GCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACT                 <  SRR4302545.8489531/94‑1 (MQ=60)
                                                                              GCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACT                 <  SRR4302545.9548094/94‑1 (MQ=60)
                                                                              GCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACT                 <  SRR4302545.10273251/94‑1 (MQ=60)
                                                                               CACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTC                <  SRR4302545.2805261/94‑1 (MQ=60)
                                                                                CCTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCT               <  SRR4302545.9778753/94‑1 (MQ=60)
                                                                                ACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCT               <  SRR4302545.9578266/94‑1 (MQ=60)
                                                                                ACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCACGATGTGCGATGCTCTGCATGAAGCGGACTCT               >  SRR4302545.6442024/1‑94 (MQ=60)
                                                                                 CTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTG              <  SRR4302545.195601/94‑1 (MQ=60)
                                                                                  TTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGG             <  SRR4302545.2843061/94‑1 (MQ=60)
                                                                                  TTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGG             <  SRR4302545.6754801/94‑1 (MQ=60)
                                                                                   TCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGT            >  SRR4302545.7130325/1‑94 (MQ=60)
                                                                                    CTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTG           >  SRR4302545.8694204/1‑94 (MQ=60)
                                                                                     TTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGA          <  SRR4302545.4560050/94‑1 (MQ=60)
                                                                                     TTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGA          >  SRR4302545.652625/1‑94 (MQ=60)
                                                                                      TCATTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAG         <  SRR4302545.4722734/94‑1 (MQ=60)
                                                                                      TCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGTAGCGGACTCTGGTGAG         <  SRR4302545.7985237/94‑1 (MQ=60)
                                                                                      TCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAG         <  SRR4302545.4722733/94‑1 (MQ=60)
                                                                                      TCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAG         >  SRR4302545.1093297/1‑94 (MQ=60)
                                                                                       CAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGG        <  SRR4302545.1749194/94‑1 (MQ=60)
                                                                                       CAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGG        >  SRR4302545.10185573/1‑94 (MQ=60)
                                                                                       CAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGG        <  SRR4302545.2932682/94‑1 (MQ=60)
                                                                                       CAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGG        >  SRR4302545.8656592/1‑94 (MQ=60)
                                                                                       CAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGG        >  SRR4302545.346550/1‑94 (MQ=60)
                                                                                        AGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGG       <  SRR4302545.9272037/94‑1 (MQ=60)
                                                                                        AGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGG       <  SRR4302545.7258258/94‑1 (MQ=60)
                                                                                        AGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGG       >  SRR4302545.10519774/1‑94 (MQ=60)
                                                                                         GTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGT      >  SRR4302545.6863812/1‑94 (MQ=60)
                                                                                          TGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTG     >  SRR4302545.1422962/1‑94 (MQ=60)
                                                                                          TGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGAG     >  SRR4302545.8428875/1‑94 (MQ=60)
                                                                                           GCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGT    <  SRR4302545.4977511/94‑1 (MQ=60)
                                                                                           GCTGGGCCGTAAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGT    <  SRR4302545.2087988/94‑1 (MQ=60)
                                                                                             TGGGCCGTTAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCA  <  SRR4302545.7727008/94‑1 (MQ=60)
                                                                                             TGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCA  <  SRR4302545.7089678/94‑1 (MQ=60)
                                                                                             TGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCA  >  SRR4302545.524769/1‑94 (MQ=60)
                                                                                             TGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCA  >  SRR4302545.5053780/1‑94 (MQ=60)
                                                                                             |                                                                                             
GCGATGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCCGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCA  >  NC_000913/144574‑144760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑