Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	144,667	C→T	R31W (CGG→TGG)	yadI →	putative PTS enzyme IIA component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	144,667	0	C	T	99.5%	770.9 / NA	218	R31W (CGG→TGG)	yadI	putative PTS enzyme IIA component
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (104/113); minor base A (1/0); total (105/113)
Fisher's exact test for biased strand distribution p-value = 4.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 221 total aligned reads displayed.

ATGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCCGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGT  >  NC_000913/144577‑144756

aTGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTgg                                                                                         <  2:2159112/93‑1 (MQ=255)

aTGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTgg                                                                                         <  2:1173941/93‑1 (MQ=255)

  gTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGc                                                                                       >  2:969532/1‑93 (MQ=255)

  gTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGc                                                                                       >  1:1172205/1‑93 (MQ=255)

   ttAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGcc                                                                                      >  1:3646114/1‑93 (MQ=255)

      ggTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTg                                                                                   >  2:1908561/1‑93 (MQ=255)

        ttGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGaa                                                                                 >  1:4719767/1‑93 (MQ=255)

         tGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAAt                                                                                <  2:677814/93‑1 (MQ=255)

          gggTAATTACCTGTCGCGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAAtt                                                                               <  2:4352750/93‑1 (MQ=255)

          gggTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAAtt                                                                               <  1:5097527/93‑1 (MQ=255)

            gTAATTCCCTGTCACGATGACCGGGCCCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCATTGCTGCGCCGTGAATTTc                                                                             <  2:3161211/93‑1 (MQ=255)

            gTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTc                                                                             <  2:118078/93‑1 (MQ=255)

            gTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTc                                                                             <  1:3951658/93‑1 (MQ=255)

             tAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCt                                                                            <  2:5214865/93‑1 (MQ=255)

                ttACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGgc                                                                         >  1:148680/1‑93 (MQ=255)

                  aCCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGgcgc                                                                       >  1:2710714/1‑93 (MQ=255)

                    cTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCgg                                                                     >  1:3541433/1‑93 (MQ=255)

                     tGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGa                                                                    >  2:867920/1‑93 (MQ=255)

                     cgTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGa                                                                    <  1:6151133/92‑1 (MQ=255)

                      gTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGAt                                                                   <  1:6425144/93‑1 (MQ=255)

                       tCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGAtt                                                                  >  2:5538537/1‑93 (MQ=255)

                          cGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAg                                                               <  1:1398193/93‑1 (MQ=255)

                           gATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGc                                                              <  2:4090793/93‑1 (MQ=255)

                           gATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGc                                                              >  1:4567042/1‑93 (MQ=255)

                               aCCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTa                                                          <  1:3306596/93‑1 (MQ=255)

                                  gggCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAata                                                       <  1:413761/93‑1 (MQ=255)

                                   ggCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAatat                                                      <  2:4926526/93‑1 (MQ=255)

                                   ggCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAatat                                                      >  2:4978148/1‑93 (MQ=255)

                                    gcgcAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATg                                                     <  2:2892708/93‑1 (MQ=255)

                                     cgcAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGc                                                    <  2:2273039/93‑1 (MQ=255)

                                     cgcAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGc                                                    <  2:3267965/93‑1 (MQ=255)

                                      gcAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCt                                                   >  1:2542637/1‑93 (MQ=255)

                                        aaGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCa                                                 >  2:1715413/1‑93 (MQ=255)

                                        aaGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCa                                                 >  1:233402/1‑93 (MQ=255)

                                        aaGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCa                                                 <  1:4819877/93‑1 (MQ=255)

                                        aaGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCa                                                 >  1:2684454/1‑93 (MQ=255)

                                         agagATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAg                                                <  1:5777036/93‑1 (MQ=255)

                                          gagaTACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGc                                               >  2:3177178/1‑93 (MQ=255)

                                          gagaTACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGc                                               <  1:5620247/93‑1 (MQ=255)

                                           agaTACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGcc                                              <  1:2262975/93‑1 (MQ=255)

                                            gaTACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCg                                             <  1:5842835/93‑1 (MQ=255)

                                            gaTACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCg                                             >  1:573291/1‑93 (MQ=255)

                                              tACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCa                                           >  2:2488535/1‑93 (MQ=255)

                                              tACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCa                                           >  2:566994/1‑93 (MQ=255)

                                              tACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCa                                           <  2:4665052/93‑1 (MQ=255)

                                               aCTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCat                                          <  1:4940670/93‑1 (MQ=255)

                                               aCTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCat                                          <  2:580569/93‑1 (MQ=255)

                                                cTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCatg                                         >  1:3111119/1‑93 (MQ=255)

                                                 tGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCatga                                        >  2:5643112/1‑93 (MQ=255)

                                                 tGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCatga                                        >  1:1785057/1‑93 (MQ=255)

                                                 tGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCatga                                        >  2:2776481/1‑93 (MQ=255)

                                                   gATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCatgatg                                      <  2:630511/93‑1 (MQ=255)

                                                    aTGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGAtgt                                     <  2:3929488/93‑1 (MQ=255)

                                                     tGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGAtgtg                                    >  1:4245933/1‑93 (MQ=255)

                                                      gcgcTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGc                                   <  1:2994619/93‑1 (MQ=255)

                                                      gcgcTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGc                                   <  2:608731/93‑1 (MQ=255)

                                                         cTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGAt                                <  2:3453782/93‑1 (MQ=255)

                                                          tGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATg                               >  2:1056375/1‑93 (MQ=255)

                                                          tGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATg                               >  1:4358512/1‑93 (MQ=255)

                                                           ggAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGc                              >  1:379601/1‑93 (MQ=255)

                                                           ggAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGc                              >  2:3320078/1‑93 (MQ=255)

                                                              gaAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGctct                           >  1:4066701/1‑93 (MQ=255)

                                                               aaaaaaCATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCATCCGCATGATGTGCGATGCTCTg                          >  1:6105256/1‑93 (MQ=255)

                                                                aaaaaCATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGc                         <  1:2157222/93‑1 (MQ=255)

                                                                aaaaaCATGGGGCACTTCTTCAGAGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGc                         >  1:1053679/1‑93 (MQ=255)

                                                                 aaaaCATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCa                        <  2:2017023/93‑1 (MQ=255)

                                                                   aaCATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATg                      >  2:4343960/1‑93 (MQ=255)

                                                                    aCATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGa                     <  1:6200257/93‑1 (MQ=255)

                                                                      aTGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAg                   >  1:202780/1‑93 (MQ=255)

                                                                      aTGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAg                   <  1:6334140/93‑1 (MQ=255)

                                                                      aTGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAg                   <  2:3378755/93‑1 (MQ=255)

                                                                      aTGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAg                   >  1:5272318/1‑93 (MQ=255)

                                                                       tGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGc                  >  1:448436/1‑93 (MQ=255)

                                                                       tGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGc                  <  1:2597044/93‑1 (MQ=255)

                                                                       tGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGc                  <  1:4596303/93‑1 (MQ=255)

                                                                       tGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGc                  <  2:135898/93‑1 (MQ=255)

                                                                           gCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGAc              <  2:1566816/93‑1 (MQ=255)

                                                                           gCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGAc              <  2:877830/93‑1 (MQ=255)

                                                                            cACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGAct             >  1:1368697/1‑93 (MQ=255)

                                                                            cACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGAct             <  1:707597/93‑1 (MQ=255)

                                                                             aCTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGActc            <  2:222825/93‑1 (MQ=255)

                                                                             aCTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGActc            <  2:5262451/93‑1 (MQ=255)

                                                                             aCTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGActc            <  2:5701681/93‑1 (MQ=255)

                                                                              cttcttCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGActct           >  1:2830753/1‑93 (MQ=255)

                                                                               ttcttcAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTg          <  2:5128778/93‑1 (MQ=255)

                                                                                tcttcAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTgg         >  1:3427404/1‑93 (MQ=255)

                                                                                tcttcAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTgg         <  2:95383/93‑1 (MQ=255)

                                                                                tcttcAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGGGCGATGCTCTTCATGAAGCGGACTCTgg         <  1:1616288/93‑1 (MQ=255)

                                                                                 cttcAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGt        >  1:4858846/1‑93 (MQ=255)

                                                                                 cttcAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGt        <  1:3845789/93‑1 (MQ=255)

                                                                                  ttcAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTg       <  2:5898081/93‑1 (MQ=255)

                                                                                   tcAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGa      >  2:3616231/1‑93 (MQ=255)

                                                                                   tcAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGa      <  1:5803276/93‑1 (MQ=255)

                                                                                     aGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAgg    >  1:416403/1‑93 (MQ=255)

                                                                                      gTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAggg   <  1:765043/93‑1 (MQ=255)

                                                                                      gTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAggg   <  1:519406/93‑1 (MQ=255)

                                                                                      gTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAggg   <  2:3532940/93‑1 (MQ=255)

                                                                                       tGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGgt  >  1:2776779/1‑93 (MQ=255)

                                                                                       tGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGgt  <  1:1886676/93‑1 (MQ=255)

                                                                                       tGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGgt  >  1:1027076/1‑93 (MQ=255)

ATGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCCGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGT  >  NC_000913/144577‑144756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCGATGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCCGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCA  >  NC_000913/144574‑144760

GCGATGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCT                                                                                               <  SRR4302537.598392/94‑1 (MQ=60)

GCGATGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCT                                                                                               <  SRR4302537.4792115/94‑1 (MQ=60)

 CGATGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTG                                                                                              >  SRR4302537.794388/1‑94 (MQ=60)

 CGATGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTG                                                                                              <  SRR4302537.1682798/94‑1 (MQ=60)

  GATGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGG                                                                                             <  SRR4302537.4904838/94‑1 (MQ=60)

   ATGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGG                                                                                            <  SRR4302537.2237201/94‑1 (MQ=60)

   ATGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGG                                                                                            >  SRR4302537.5538870/1‑94 (MQ=60)

    TGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGC                                                                                           >  SRR4302537.1180363/1‑94 (MQ=60)

     GTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCC                                                                                          >  SRR4302537.3671781/1‑94 (MQ=60)

      TTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCG                                                                                         <  SRR4302537.3024706/94‑1 (MQ=60)

        AGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTG                                                                                       >  SRR4302537.6216909/1‑94 (MQ=60)

         GGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGA                                                                                      >  SRR4302537.5646613/1‑94 (MQ=60)

          GTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAA                                                                                     >  SRR4302537.4752402/1‑94 (MQ=60)

           TTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAAT                                                                                    <  SRR4302537.6159826/94‑1 (MQ=60)

            TGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATT                                                                                   <  SRR4302537.3596239/94‑1 (MQ=60)

             GGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTT                                                                                  <  SRR4302537.5132632/94‑1 (MQ=60)

               GTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCT                                                                                <  SRR4302537.3979299/94‑1 (MQ=60)

                TAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTG                                                                               <  SRR4302537.4424001/94‑1 (MQ=60)

                 AATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGG                                                                              >  SRR4302537.883868/1‑94 (MQ=60)

                  ATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGC                                                                             >  SRR4302537.149743/1‑94 (MQ=60)

                    TACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGC                                                                           >  SRR4302537.2729646/1‑94 (MQ=60)

                    TACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGC                                                                           <  SRR4302537.1514197/94‑1 (MQ=60)

                      CCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGG                                                                         >  SRR4302537.3566450/1‑94 (MQ=60)

                      CCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGG                                                                         >  SRR4302537.4360032/1‑94 (MQ=60)

                        CGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGAT                                                                       <  SRR4302537.6193442/94‑1 (MQ=60)

                         GTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATT                                                                      <  SRR4302537.6468805/94‑1 (MQ=60)

                          TCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTA                                                                     <  SRR4302537.1730458/94‑1 (MQ=60)

                          TCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTA                                                                     <  SRR4302537.3441780/94‑1 (MQ=60)

                           CACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAA                                                                    >  SRR4302537.4608095/1‑94 (MQ=60)

                             CGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGC                                                                  >  SRR4302537.4598653/1‑94 (MQ=60)

                             CGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGC                                                                  <  SRR4302537.1407939/94‑1 (MQ=60)

                              GATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTGAGCT                                                                 >  SRR4302537.5210475/1‑94 (MQ=60)

                              GATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCT                                                                 >  SRR4302537.4946147/1‑94 (MQ=60)

                                TGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCT                                                               <  SRR4302537.3784465/94‑1 (MQ=60)

                                TGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCT                                                               <  SRR4302537.627196/94‑1 (MQ=60)

                                 GACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTA                                                              <  SRR4302537.4210700/94‑1 (MQ=60)

                                 GACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTA                                                              >  SRR4302537.5831795/1‑94 (MQ=60)

                                 GACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTA                                                              >  SRR4302537.1044513/1‑94 (MQ=60)

                                  ACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAA                                                             <  SRR4302537.3329973/94‑1 (MQ=60)

                                    CGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATA                                                           <  SRR4302537.3930796/94‑1 (MQ=60)

                                     GGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATAT                                                          <  SRR4302537.416627/94‑1 (MQ=60)

                                        CGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCT                                                       >  SRR4302537.2560460/1‑94 (MQ=60)

                                         GCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTC                                                      >  SRR4302537.1822839/1‑94 (MQ=60)

                                         GCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTC                                                      >  SRR4302537.3457457/1‑94 (MQ=60)

                                          CAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCA                                                     >  SRR4302537.235051/1‑94 (MQ=60)

                                          CAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCA                                                     >  SRR4302537.2703197/1‑94 (MQ=60)

                                           AAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAG                                                    <  SRR4302537.4853156/94‑1 (MQ=60)

                                            AGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGC                                                   <  SRR4302537.5816636/94‑1 (MQ=60)

                                             GAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCC                                                  <  SRR4302537.5658677/94‑1 (MQ=60)

                                              AGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCG                                                 >  SRR4302537.5787387/1‑94 (MQ=60)

                                              AGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCG                                                 <  SRR4302537.2278944/94‑1 (MQ=60)

                                              AGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCG                                                 >  SRR4302537.577229/1‑94 (MQ=60)

                                               GATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGC                                                <  SRR4302537.5882896/94‑1 (MQ=60)

                                                ATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCA                                               >  SRR4302537.2717267/1‑94 (MQ=60)

                                                  ACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATG                                             >  SRR4302537.3132846/1‑94 (MQ=60)

                                                  ACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATG                                             <  SRR4302537.4974743/94‑1 (MQ=60)

                                                  ACTGGATGCGCTGGAGAAAAAACATGGGGAACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATG                                             <  SRR4302537.2375057/94‑1 (MQ=60)

                                                   CTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGA                                            >  SRR4302537.1797506/1‑94 (MQ=60)

                                                   CTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGA                                            >  SRR4302537.657220/1‑94 (MQ=60)

                                                     GGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATG                                          >  SRR4302537.732259/1‑94 (MQ=60)

                                                       ATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTTAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTG                                        >  SRR4302537.1448749/1‑94 (MQ=60)

                                                       ATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTG                                        >  SRR4302537.4275441/1‑94 (MQ=60)

                                                         GCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCG                                      <  SRR4302537.3015508/94‑1 (MQ=60)

                                                          CGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGA                                     >  SRR4302537.1993820/1‑94 (MQ=60)

                                                          CGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGA                                     >  SRR4302537.3008475/1‑94 (MQ=60)

                                                          CGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGA                                     >  SRR4302537.4918070/1‑94 (MQ=60)

                                                            CTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATG                                   <  SRR4302537.1404969/94‑1 (MQ=60)

                                                            CTGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATG                                   >  SRR4302537.4388674/1‑94 (MQ=60)

                                                             TGGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGC                                  >  SRR4302537.382231/1‑94 (MQ=60)

                                                              GGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCT                                 <  SRR4302537.5235475/94‑1 (MQ=60)

                                                              GGAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCT                                 <  SRR4302537.2677942/94‑1 (MQ=60)

                                                               GAGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTC                                >  SRR4302537.1192466/1‑94 (MQ=60)

                                                                AGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCT                               <  SRR4302537.3026372/94‑1 (MQ=60)

                                                                AGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCT                               <  SRR4302537.5697088/94‑1 (MQ=60)

                                                                AGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCT                               <  SRR4302537.4532594/94‑1 (MQ=60)

                                                                AGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCT                               >  SRR4302537.4095115/1‑94 (MQ=60)

                                                                AGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCT                               <  SRR4302537.486307/94‑1 (MQ=60)

                                                                AGAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCT                               >  SRR4302537.5882306/1‑94 (MQ=60)

                                                                 GAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCATCCGCATGATGTGCGATGCTCTG                              >  SRR4302537.6147200/1‑94 (MQ=60)

                                                                 GAAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTG                              <  SRR4302537.2585913/94‑1 (MQ=60)

                                                                  AAAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGC                             >  SRR4302537.410602/1‑94 (MQ=60)

                                                                  AAAAAACATGGGGCACTTCTTCAGAGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGC                             >  SRR4302537.1060949/1‑94 (MQ=60)

                                                                   AAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCA                            >  SRR4302537.6394413/1‑94 (MQ=60)

                                                                   AAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCA                            <  SRR4302537.2172426/94‑1 (MQ=60)

                                                                   AAAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCA                            <  SRR4302537.3170624/94‑1 (MQ=60)

                                                                    AAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCAT                           <  SRR4302537.4026770/94‑1 (MQ=60)

                                                                    AAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCAT                           >  SRR4302537.2772023/1‑94 (MQ=60)

                                                                    AAAACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCAT                           >  SRR4302537.2628232/1‑94 (MQ=60)

                                                                      AACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGA                         <  SRR4302537.6058634/94‑1 (MQ=60)

                                                                       ACATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAA                        <  SRR4302537.6242989/94‑1 (MQ=60)

                                                                        CATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAG                       >  SRR4302537.204233/1‑94 (MQ=60)

                                                                        CATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAG                       >  SRR4302537.5308601/1‑94 (MQ=60)

                                                                         ATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGC                      <  SRR4302537.6377447/94‑1 (MQ=60)

                                                                         ATGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGC                      >  SRR4302537.451545/1‑94 (MQ=60)

                                                                          TGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCG                     <  SRR4302537.4628115/94‑1 (MQ=60)

                                                                          TGGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCG                     <  SRR4302537.2615209/94‑1 (MQ=60)

                                                                           GGGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGG                    <  SRR4302537.650182/94‑1 (MQ=60)

                                                                            GGGCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGA                   >  SRR4302537.2842992/1‑94 (MQ=60)

                                                                              GCACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACT                 >  SRR4302537.1378234/1‑94 (MQ=60)

                                                                               CACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTC                >  SRR4302537.1408178/1‑94 (MQ=60)

                                                                               CACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTC                <  SRR4302537.712438/94‑1 (MQ=60)

                                                                               CACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTC                <  SRR4302537.2134607/94‑1 (MQ=60)

                                                                                ACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCT               >  SRR4302537.2850534/1‑94 (MQ=60)

                                                                                ACTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCT               <  SRR4302537.4766879/94‑1 (MQ=60)

                                                                                 CTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGTATGATGTGCGATGCTCTGCATGAAGCGGACTCTG              <  SRR4302537.4720418/94‑1 (MQ=60)

                                                                                 CTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTG              <  SRR4302537.4143669/94‑1 (MQ=60)

                                                                                 CTTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTG              >  SRR4302537.1168497/1‑94 (MQ=60)

                                                                                  TTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGG             <  SRR4302537.507115/94‑1 (MQ=60)

                                                                                  TTCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGG             >  SRR4302537.3451662/1‑94 (MQ=60)

                                                                                   TCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGT            >  SRR4302537.4892370/1‑94 (MQ=60)

                                                                                   TCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGT            >  SRR4302537.4574940/1‑94 (MQ=60)

                                                                                   TCTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGGGCGATGCTCTTCATGAAGCGGACTCTGGT            <  SRR4302537.1627579/94‑1 (MQ=60)

                                                                                    CTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTG           <  SRR4302537.3872714/94‑1 (MQ=60)

                                                                                    CTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTG           <  SRR4302537.2054293/94‑1 (MQ=60)

                                                                                    CTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTG           >  SRR4302537.358246/1‑94 (MQ=60)

                                                                                    CTTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTG           >  SRR4302537.3859128/1‑94 (MQ=60)

                                                                                     TTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGA          >  SRR4302537.2332863/1‑94 (MQ=60)

                                                                                     TTCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGA          >  SRR4302537.4181029/1‑94 (MQ=60)

                                                                                      TCAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAG         <  SRR4302537.5843071/94‑1 (MQ=60)

                                                                                       CAGTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGG        >  SRR4302537.419294/1‑94 (MQ=60)

                                                                                         GTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGT      <  SRR4302537.770279/94‑1 (MQ=60)

                                                                                         GTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGT      >  SRR4302537.1034145/1‑94 (MQ=60)

                                                                                         GTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGT      >  SRR4302537.2796192/1‑94 (MQ=60)

                                                                                         GTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGT      <  SRR4302537.5885732/94‑1 (MQ=60)

                                                                                         GTGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGT      <  SRR4302537.522979/94‑1 (MQ=60)

                                                                                          TGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTG     >  SRR4302537.697617/1‑94 (MQ=60)

                                                                                          TGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTG     >  SRR4302537.6011040/1‑94 (MQ=60)

                                                                                          TGCTGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTG     <  SRR4302537.1899796/94‑1 (MQ=60)

                                                                                             TGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCA  <  SRR4302537.4828020/94‑1 (MQ=60)

GCGATGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCACTTCTTCAGTGCCGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCA  >  NC_000913/144574‑144760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`