Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	647	270.1	21258240	98.4%	20918108	94.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	159,448	A→G	V222A (GTG→GCG)	yadB ←	putative tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	159,448	0	A	G	98.9%	1510.5 / 1.8	444	V222A (GTG→GCG)	yadB	putative tRNA synthetase
Reads supporting (aligned to +/- strand): ref base A (4/1); new base G (225/214); total (229/215)
Fisher's exact test for biased strand distribution p-value = 3.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.51e-01

Only 100 of 461 total aligned reads displayed.

GATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCACTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCTGGA  >  NC_000913/159356‑159538

  tCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCtt                                                                                         >  1:242968/1‑94 (MQ=255)

     ccTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTcc                                                                                      <  2:9750683/94‑1 (MQ=255)

     ccTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTcc                                                                                      >  2:5775306/1‑94 (MQ=255)

     ccTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTcc                                                                                      >  2:5125233/1‑94 (MQ=255)

     ccTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTcc                                                                                      >  2:2805382/1‑94 (MQ=255)

      cTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCa                                                                                     <  2:6452737/94‑1 (MQ=255)

      cTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCa                                                                                     <  2:2304871/94‑1 (MQ=255)

      cTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCa                                                                                     <  1:9564159/94‑1 (MQ=255)

       tttCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAg                                                                                    <  1:8539859/94‑1 (MQ=255)

          cGGCAACGCAGGCACATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCa                                                                                 >  2:1433417/1‑94 (MQ=255)

              aaCGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCaaaaa                                                                             >  1:2131697/1‑94 (MQ=255)

                cGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGc                                                                           >  2:2653014/1‑94 (MQ=255)

                  cAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAACGCTg                                                                         >  1:7368620/1‑94 (MQ=255)

                  cAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTg                                                                         >  2:3948973/1‑94 (MQ=255)

                   aGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTgg                                                                        >  1:5352763/1‑94 (MQ=255)

                    ggCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGt                                                                       <  2:1267686/94‑1 (MQ=255)

                     gcgcATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTa                                                                      <  1:2998632/94‑1 (MQ=255)

                      cgcATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTAc                                                                     >  2:6802760/1‑94 (MQ=255)

                      cgcATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTAc                                                                     >  1:3186279/1‑94 (MQ=255)

                       gcATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACa                                                                    <  1:3945706/94‑1 (MQ=255)

                        cATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAg                                                                   >  1:6243135/1‑94 (MQ=255)

                         aTGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGc                                                                  >  2:4274954/1‑94 (MQ=255)

                          tGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCg                                                                 <  1:3545356/94‑1 (MQ=255)

                          tGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCg                                                                 <  1:9505268/94‑1 (MQ=255)

                            aTTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCgag                                                               >  2:6896339/1‑94 (MQ=255)

                             ttCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCgaga                                                              >  1:9883391/1‑94 (MQ=255)

                              tCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGAt                                                             >  2:8500551/1‑94 (MQ=255)

                              tCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGAt                                                             <  2:6492298/94‑1 (MQ=255)

                              tCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGAt                                                             <  1:6356407/94‑1 (MQ=255)

                               cTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGAtt                                                            <  2:7263128/94‑1 (MQ=255)

                               cTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGAtt                                                            <  1:6990458/94‑1 (MQ=255)

                               cTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGAtt                                                            >  1:5508316/1‑94 (MQ=255)

                                tGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACGGCGAGAttt                                                           >  1:7975504/1‑94 (MQ=255)

                                tGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCACTTTCCAGCCAAAAAGCTGGTACAGCGAGAttt                                                           >  2:2118957/1‑94 (MQ=255)

                                 gCTTGTAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTg                                                          <  1:3436060/94‑1 (MQ=255)

                                 gCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTg                                                          >  2:10462034/1‑94 (MQ=255)

                                 gCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTg                                                          <  1:5668696/94‑1 (MQ=255)

                                  cTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGc                                                         >  1:4778973/1‑94 (MQ=255)

                                   ttGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGcc                                                        >  1:1920380/1‑94 (MQ=255)

                                    tGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAACAGCTGGTACAGCGAGATTTGCCt                                                       <  2:1839881/94‑1 (MQ=255)

                                    tGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCt                                                       <  2:916289/94‑1 (MQ=255)

                                    tGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCt                                                       <  2:9512006/94‑1 (MQ=255)

                                     ggAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCtt                                                      <  2:7863587/94‑1 (MQ=255)

                                     ggAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCtt                                                      <  2:5002564/94‑1 (MQ=255)

                                      gAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTa                                                     >  2:10128148/1‑94 (MQ=255)

                                       aaaGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTAc                                                    <  1:659892/94‑1 (MQ=255)

                                         aGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTg                                                  >  2:8100874/1‑94 (MQ=255)

                                           tttAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGtt                                                <  2:323848/94‑1 (MQ=255)

                                             tAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTgt                                              >  2:421631/1‑93 (MQ=255)

                                             tAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTgg                                              >  2:8771402/1‑94 (MQ=255)

                                              aGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGt                                             >  2:8049189/1‑94 (MQ=255)

                                               gcgcCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGtt                                            >  1:2231074/1‑94 (MQ=255)

                                                cgcCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTc                                           <  2:200818/94‑1 (MQ=255)

                                                cgcCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTc                                           >  2:5325599/1‑94 (MQ=255)

                                                cgcCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTc                                           <  2:7034958/94‑1 (MQ=255)

                                                  ccTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCaa                                         >  2:1619378/1‑94 (MQ=255)

                                                  ccTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCaa                                         >  2:7459732/1‑94 (MQ=255)

                                                   cTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAat                                        >  1:7120219/1‑94 (MQ=255)

                                                   cTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAat                                        >  2:6393697/1‑94 (MQ=255)

                                                   cTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAat                                        >  2:308593/1‑94 (MQ=255)

                                                      gtgGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAatcag                                     <  2:4642916/94‑1 (MQ=255)

                                                       tgGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAatcaga                                    <  1:9315625/94‑1 (MQ=255)

                                                        ggATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAatcagat                                   <  1:1809112/94‑1 (MQ=255)

                                                           ttAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAatcagatcag                                <  1:10580475/94‑1 (MQ=255)

                                                           ttAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAatcagatcag                                >  1:9166242/1‑94 (MQ=255)

                                                             aaGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGcc                              >  2:6597414/1‑94 (MQ=255)

                                                                 gcCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCAc                          >  1:100453/1‑94 (MQ=255)

                                                                 gcCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCAc                          >  1:7575252/1‑94 (MQ=255)

                                                                 gcCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCAc                          <  1:2565984/94‑1 (MQ=255)

                                                                  ccAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGCGATTTGCCTTACTGTTGGTTCAATCAGATCAGACACACg                         >  1:9259707/1‑94 (MQ=255)

                                                                   cAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGc                        >  2:5154740/1‑94 (MQ=255)

                                                                    aGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCTATCAGATCAGCCCCACGCa                       >  2:10298753/1‑94 (MQ=255)

                                                                    aGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCa                       <  1:10242565/94‑1 (MQ=255)

                                                                    aGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCa                       >  1:1400974/1‑94 (MQ=255)

                                                                    aGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCa                       >  1:3820933/1‑94 (MQ=255)

                                                                    aGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCa                       >  2:9224010/1‑94 (MQ=255)

                                                                      cGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACt                     >  1:4231195/1‑94 (MQ=255)

                                                                       ggCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTa                    <  1:1565126/94‑1 (MQ=255)

                                                                        gCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTAt                   >  1:2883696/1‑94 (MQ=255)

                                                                         cAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTAtt                  >  1:1152630/1‑94 (MQ=255)

                                                                          aGATGAATGTAATCTGTCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTAttt                 <  2:6232445/94‑1 (MQ=255)

                                                                          aGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTAttt                 >  1:7709902/1‑94 (MQ=255)

                                                                          aGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTAttt                 >  2:4446671/1‑94 (MQ=255)

                                                                            aTGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCt               >  2:2536874/1‑94 (MQ=255)

                                                                             tGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTTCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTg              <  1:10447477/94‑1 (MQ=255)

                                                                              gAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGt             >  1:6864896/1‑94 (MQ=255)

                                                                                aTGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTaa           >  2:3734885/1‑94 (MQ=255)

                                                                                aTGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTaa           <  1:9725053/94‑1 (MQ=255)

                                                                                 tGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAAc          <  2:7717874/94‑1 (MQ=255)

                                                                                  gTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACg         <  2:3402526/94‑1 (MQ=255)

                                                                                  gTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACg         >  1:6029950/1‑94 (MQ=255)

                                                                                   tAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGc        >  2:8361476/1‑94 (MQ=255)

                                                                                   tAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGc        <  2:8987422/94‑1 (MQ=255)

                                                                                     aTCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGccc      >  2:2388935/1‑94 (MQ=255)

                                                                                      tCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCt     <  1:1695566/94‑1 (MQ=255)

                                                                                      tCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCt     <  1:3356064/94‑1 (MQ=255)

                                                                                       cgggCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCTg    <  2:5984180/92‑1 (MQ=255)

                                                                                        tGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCTgg   <  2:569302/94‑1 (MQ=255)

                                                                                        tGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCTgg   <  2:8681443/94‑1 (MQ=255)

                                                                                         ggCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACCGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCTGGa  <  1:4705119/94‑1 (MQ=255)

GATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCACTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCTGGA  >  NC_000913/159356‑159538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 229 total aligned reads displayed.

GGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCACTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCTGGAAAT  >  NC_000913/159355‑159541

GGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCG                                                                                               <  SRR4302545.10296594/94‑1 (MQ=60)

 GATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGC                                                                                              >  SRR4302545.3324326/1‑94 (MQ=60)

 GATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGC                                                                                              >  SRR4302545.1799246/1‑94 (MQ=60)

 GATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCAC                                                                                              >  SRR4302545.1721903/1‑94 (MQ=60)

  ATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCT                                                                                             >  SRR4302545.10562085/1‑94 (MQ=60)

  ATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCT                                                                                             >  SRR4302545.10433/1‑94 (MQ=60)

  ATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCT                                                                                             <  SRR4302545.2821730/94‑1 (MQ=60)

   TCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGGGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTT                                                                                            <  SRR4302545.6482829/94‑1 (MQ=60)

   TCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTT                                                                                            >  SRR4302545.247972/1‑94 (MQ=60)

   TCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTT                                                                                            <  SRR4302545.7016752/94‑1 (MQ=60)

     GCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGGATGTAATCTGGCGCTTTC                                                                                          >  SRR4302545.3702110/1‑94 (MQ=60)

     GCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTC                                                                                          >  SRR4302545.9780695/1‑94 (MQ=60)

       CTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCA                                                                                        >  SRR4302545.787651/1‑94 (MQ=60)

       CTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCA                                                                                        >  SRR4302545.187954/1‑94 (MQ=60)

       CTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCA                                                                                        <  SRR4302545.9846079/94‑1 (MQ=60)

       CTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCA                                                                                        >  SRR4302545.5729798/1‑94 (MQ=60)

        TTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAG                                                                                       <  SRR4302545.8786765/94‑1 (MQ=60)

        TTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAG                                                                                       <  SRR4302545.9930629/94‑1 (MQ=60)

         TTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGC                                                                                      <  SRR4302545.1980679/94‑1 (MQ=60)

          TCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCC                                                                                     >  SRR4302545.7330664/1‑94 (MQ=60)

           CGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCA                                                                                    <  SRR4302545.3948733/94‑1 (MQ=60)

           CGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCA                                                                                    <  SRR4302545.4833190/94‑1 (MQ=60)

             GCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAA                                                                                  <  SRR4302545.2436439/94‑1 (MQ=60)

              CAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAA                                                                                 >  SRR4302545.7715525/1‑94 (MQ=60)

               AACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAA                                                                                <  SRR4302545.7730313/94‑1 (MQ=60)

               AACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAA                                                                                >  SRR4302545.2183976/1‑94 (MQ=60)

                ACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAG                                                                               <  SRR4302545.6629115/94‑1 (MQ=60)

                ACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAG                                                                               <  SRR4302545.3052664/94‑1 (MQ=60)

                ACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAG                                                                               <  SRR4302545.3019263/94‑1 (MQ=60)

                  GCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCT                                                                             >  SRR4302545.1503525/1‑94 (MQ=60)

                  GCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCT                                                                             <  SRR4302545.1869910/94‑1 (MQ=60)

                  GCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCT                                                                             <  SRR4302545.3468927/94‑1 (MQ=60)

                   CAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAACGCTG                                                                            >  SRR4302545.7576164/1‑94 (MQ=60)

                   CAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTG                                                                            >  SRR4302545.5425779/1‑94 (MQ=60)

                    atgccCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGTCGCTTTCCAGCCAAAAAGCTGG                                                                           <  SRR4302545.445466/89‑1 (MQ=60)

                    AGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGG                                                                           <  SRR4302545.10667599/94‑1 (MQ=60)

                    AGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGG                                                                           >  SRR4302545.10770082/1‑94 (MQ=60)

                     GGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGT                                                                          <  SRR4302545.5418848/94‑1 (MQ=60)

                     GGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGT                                                                          <  SRR4302545.4196778/94‑1 (MQ=60)

                      GCGCATGATTCTGCTTGTAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTGTCCAGCCAAAAAGCTGGTA                                                                         >  SRR4302545.9659373/1‑94 (MQ=60)

                      GCGCATGATTCTGCTTGGAAGTTTTAGCGCCTTGTGAATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTA                                                                         <  SRR4302545.9513119/94‑1 (MQ=60)

                      GCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTA                                                                         <  SRR4302545.3071771/94‑1 (MQ=60)

                      GCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTA                                                                         >  SRR4302545.3586277/1‑94 (MQ=60)

                      GCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTA                                                                         <  SRR4302545.10558120/94‑1 (MQ=60)

                      GCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTA                                                                         <  SRR4302545.7466508/94‑1 (MQ=60)

                      GCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTA                                                                         <  SRR4302545.9513120/94‑1 (MQ=60)

                        GCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACA                                                                       <  SRR4302545.10526127/94‑1 (MQ=60)

                        GCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACA                                                                       <  SRR4302545.4044266/94‑1 (MQ=60)

                        GCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACA                                                                       >  SRR4302545.5702024/1‑94 (MQ=60)

                         CATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAG                                                                      >  SRR4302545.6413294/1‑94 (MQ=60)

                         CATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAG                                                                      >  SRR4302545.2804490/1‑94 (MQ=60)

                          ATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGC                                                                     <  SRR4302545.3023797/94‑1 (MQ=60)

                          ATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGC                                                                     >  SRR4302545.4188427/1‑94 (MQ=60)

                          ATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGC                                                                     <  SRR4302545.10352367/94‑1 (MQ=60)

                           TGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCG                                                                    <  SRR4302545.3632118/94‑1 (MQ=60)

                           TGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCG                                                                    >  SRR4302545.9525705/1‑94 (MQ=60)

                           TGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCG                                                                    <  SRR4302545.7084635/94‑1 (MQ=60)

                           TGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCG                                                                    <  SRR4302545.7084636/94‑1 (MQ=60)

                             ATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAG                                                                  >  SRR4302545.444705/1‑94 (MQ=60)

                              TTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGA                                                                 >  SRR4302545.8454621/1‑94 (MQ=60)

                               TCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGAT                                                                >  SRR4302545.575062/1‑94 (MQ=60)

                               TCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGAT                                                                <  SRR4302545.6108418/94‑1 (MQ=60)

                               TCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGAT                                                                <  SRR4302545.6530060/94‑1 (MQ=60)

                                CTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATT                                                               >  SRR4302545.5653569/1‑94 (MQ=60)

                                CTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATT                                                               <  SRR4302545.9067695/94‑1 (MQ=60)

                                CTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGAGGCAGATGAATGTAATCTGGCGCTTTTCAGCCAAAAAGCTGGTACAGCGAGATT                                                               >  SRR4302545.3886338/1‑94 (MQ=60)

                                 TGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACGGCGAGATTT                                                              >  SRR4302545.8202268/1‑94 (MQ=60)

                                 TGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTT                                                              >  SRR4302545.4964767/1‑94 (MQ=60)

                                 TGCTTGGAAAGTTTAGCGCCTTGTGGATAAAGCTCCAGCTGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTT                                                              <  SRR4302545.5126477/94‑1 (MQ=60)

                                  GCTTGTAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTG                                                             <  SRR4302545.3520054/94‑1 (MQ=60)

                                  GCTTGGAAATTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTG                                                             <  SRR4302545.5819997/94‑1 (MQ=60)

                                  GCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGTCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTG                                                             <  SRR4302545.6393414/94‑1 (MQ=60)

                                  GCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTG                                                             <  SRR4302545.3520053/94‑1 (MQ=60)

                                  GCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTG                                                             <  SRR4302545.5819995/94‑1 (MQ=60)

                                  GCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTG                                                             <  SRR4302545.10141396/94‑1 (MQ=60)

                                   CTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGC                                                            >  SRR4302545.4901149/1‑94 (MQ=60)

                                   CTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGC                                                            <  SRR4302545.9557271/94‑1 (MQ=60)

                                    TTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCC                                                           >  SRR4302545.1967847/1‑94 (MQ=60)

                                    TTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCC                                                           >  SRR4302545.10028789/1‑94 (MQ=60)

                                    TTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCC                                                           <  SRR4302545.6470932/94‑1 (MQ=60)

                                     TGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCT                                                          <  SRR4302545.2578487/94‑1 (MQ=60)

                                      GGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTT                                                         >  SRR4302545.10913117/1‑94 (MQ=60)

                                      GGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTT                                                         >  SRR4302545.3264272/1‑94 (MQ=60)

                                      GGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTT                                                         <  SRR4302545.1777274/94‑1 (MQ=60)

                                      GGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTT                                                         >  SRR4302545.2207761/1‑94 (MQ=60)

                                      GGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGAGAGATTTGCCTT                                                         >  SRR4302545.10913116/1‑94 (MQ=60)

                                        AAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTAC                                                       <  SRR4302545.674590/94‑1 (MQ=60)

                                        AAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTAC                                                       >  SRR4302545.10181749/1‑94 (MQ=60)

                                        AAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTAC                                                       >  SRR4302545.191304/1‑94 (MQ=60)

                                        AAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTAC                                                       >  SRR4302545.10284415/1‑94 (MQ=60)

                                        AAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTAC                                                       <  SRR4302545.1592972/94‑1 (MQ=60)

                                         AAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACT                                                      >  SRR4302545.9790152/1‑94 (MQ=60)

                                          AGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTG                                                     >  SRR4302545.2225458/1‑94 (MQ=60)

                                           GTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGT                                                    <  SRR4302545.2697590/94‑1 (MQ=60)

                                           GTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGT                                                    <  SRR4302545.9794719/94‑1 (MQ=60)

                                              TAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGG                                                 >  SRR4302545.1072300/1‑94 (MQ=60)

                                               AGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGT                                                <  SRR4302545.1885522/94‑1 (MQ=60)

                                                GCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTCCTGTTGGTT                                               <  SRR4302545.2618524/94‑1 (MQ=60)

                                                GCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTT                                               <  SRR4302545.4889770/94‑1 (MQ=60)

                                                GCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTT                                               >  SRR4302545.2285503/1‑94 (MQ=60)

                                                 CGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTT                                              >  SRR4302545.2104567/1‑94 (MQ=60)

                                                 CGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTC                                              >  SRR4302545.1187356/1‑94 (MQ=60)

                                                 CGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTC                                              >  SRR4302545.2571930/1‑94 (MQ=60)

                                                   CCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAA                                            <  SRR4302545.7778120/94‑1 (MQ=60)

                                                   CCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAA                                            <  SRR4302545.10902581/94‑1 (MQ=60)

                                                    CTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAAT                                           >  SRR4302545.7317568/1‑94 (MQ=60)

                                                    CTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAAT                                           <  SRR4302545.1010560/94‑1 (MQ=60)

                                                     TTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATC                                          <  SRR4302545.8099352/94‑1 (MQ=60)

                                                     TTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATC                                          <  SRR4302545.8099353/94‑1 (MQ=60)

                                                      TGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCA                                         <  SRR4302545.9338273/94‑1 (MQ=60)

                                                      TGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCA                                         <  SRR4302545.2456147/94‑1 (MQ=60)

                                                      TGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCA                                         <  SRR4302545.3300790/94‑1 (MQ=60)

                                                       GTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAG                                        <  SRR4302545.10636344/94‑1 (MQ=60)

                                                       GTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAG                                        <  SRR4302545.1497708/94‑1 (MQ=60)

                                                        TGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGA                                       <  SRR4302545.9588746/94‑1 (MQ=60)

                                                        TGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGA                                       <  SRR4302545.2473038/94‑1 (MQ=60)

                                                         GGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGAT                                      >  SRR4302545.6478365/1‑94 (MQ=60)

                                                         GGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGAT                                      >  SRR4302545.10081442/1‑94 (MQ=60)

                                                         GGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGAT                                      <  SRR4302545.5508445/94‑1 (MQ=60)

                                                         GGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGAT                                      <  SRR4302545.1852910/94‑1 (MQ=60)

                                                           gtaAAGCGCCAGCGGCAGATGAATGTAAGCTAGCGATTTCCAAACAAAAAGCTGGTACAGCGAGATTTGCCTGAATGTTAATTCAATCAGAGCA                                    <  SRR4302545.8764255/91‑1 (MQ=33)

                                                           ATTAAGCGCCAGCGGCAGATGAATGTAATCTNGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGNTCA                                    <  SRR4302545.4111485/94‑1 (MQ=60)

                                                           ATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCA                                    <  SRR4302545.1392221/94‑1 (MQ=60)

                                                           ATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCA                                    <  SRR4302545.2965986/94‑1 (MQ=60)

                                                            TTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAG                                   <  SRR4302545.10892122/94‑1 (MQ=60)

                                                            TTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAG                                   >  SRR4302545.9435230/1‑94 (MQ=60)

                                                            TTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAG                                   <  SRR4302545.2105881/94‑1 (MQ=60)

                                                            TTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAG                                   <  SRR4302545.3588912/94‑1 (MQ=60)

                                                             TAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGC                                  <  SRR4302545.8030362/94‑1 (MQ=60)

                                                             TAAGCGCCAGCGGCAGATGAATGTAATCTGGCACTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGC                                  <  SRR4302545.7357126/94‑1 (MQ=60)

                                                              AAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCC                                 >  SRR4302545.1707055/1‑94 (MQ=60)

                                                               AGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATTAGATCAGCCC                                >  SRR4302545.3272211/1‑94 (MQ=60)

                                                               AGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCC                                >  SRR4302545.6833815/1‑94 (MQ=60)

                                                                GCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCC                               >  SRR4302545.1145023/1‑94 (MQ=60)

                                                                GCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCC                               >  SRR4302545.9641581/1‑94 (MQ=60)

                                                                 CGCCGGCGGAAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTTCCTTACTGTTGGTTCAATCAGATCAGCCCCA                              <  SRR4302545.7448545/94‑1 (MQ=60)

                                                                  GCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCAC                             <  SRR4302545.6328501/94‑1 (MQ=60)

                                                                  GCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCAC                             >  SRR4302545.7789356/1‑94 (MQ=60)

                                                                  GCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCAC                             >  SRR4302545.102763/1‑94 (MQ=60)

                                                                  GCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCAC                             <  SRR4302545.1340820/94‑1 (MQ=60)

                                                                  GCCAGCGGCAGATGAATGTAATCTGGCACTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGACTTACTGTTGGTTCAATAAGATAAGCCCCAC                             >  SRR4302545.4548366/1‑94 (MQ=60)

                                                                   ctgGCGGCAGATGAGTGTAATCTGGCGCTTACCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACG                            <  SRR4302545.7298784/91‑1 (MQ=60)

                                                                   GCAGCGGCAGATTAATTTAATCGGGCGATTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACG                            <  SRR4302545.2221939/94‑1 (MQ=60)

                                                                   CCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGCGATTTGCCTTACTGTTGGTTCAATCAGATCAGACACACG                            >  SRR4302545.9531185/1‑94 (MQ=60)

                                                                   CCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACG                            <  SRR4302545.7298791/94‑1 (MQ=60)

                                                                   CCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACG                            >  SRR4302545.9531184/1‑94 (MQ=60)

                                                                    CAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGC                           >  SRR4302545.8491511/1‑94 (MQ=60)

                                                                     AGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCC                          >  SRR4302545.9155270/1‑94 (MQ=60)

                                                                     AGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCA                          >  SRR4302545.1432532/1‑94 (MQ=60)

                                                                     AGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCA                          >  SRR4302545.3916033/1‑94 (MQ=60)

                                                                      GCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCAC                         >  SRR4302545.3913459/1‑94 (MQ=60)

                                                                       CGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACT                        >  SRR4302545.4336952/1‑94 (MQ=60)

                                                                       CGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACT                        >  SRR4302545.9576021/1‑94 (MQ=60)

                                                                       CGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACT                        <  SRR4302545.5650085/94‑1 (MQ=60)

                                                                        GGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTA                       <  SRR4302545.7143446/94‑1 (MQ=60)

                                                                        GGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTA                       <  SRR4302545.1601939/94‑1 (MQ=60)

                                                                         GCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTAT                      >  SRR4302545.9881317/1‑94 (MQ=60)

                                                                         GCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTAT                      <  SRR4302545.5874518/94‑1 (MQ=60)

                                                                         GCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTAT                      >  SRR4302545.2953506/1‑94 (MQ=60)

                                                                         GCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTAT                      >  SRR4302545.4795689/1‑94 (MQ=60)

                                                                          CAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATT                     >  SRR4302545.10074117/1‑94 (MQ=60)

                                                                          CAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATT                     <  SRR4302545.3172485/94‑1 (MQ=60)

                                                                          CAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATT                     >  SRR4302545.7796670/1‑94 (MQ=60)

                                                                          CAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATT                     >  SRR4302545.1177919/1‑94 (MQ=60)

                                                                           AGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTT                    >  SRR4302545.7928165/1‑94 (MQ=60)

                                                                            GATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTC                   <  SRR4302545.1749629/94‑1 (MQ=60)

                                                                            GATGAAAGTACTCTGGCGTTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTC                   <  SRR4302545.3154560/94‑1 (MQ=60)

                                                                             ATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCT                  <  SRR4302545.6637558/94‑1 (MQ=60)

                                                                              TGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTTCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTG                 <  SRR4302545.10755376/94‑1 (MQ=60)

                                                                              TGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTG                 >  SRR4302545.6569460/1‑94 (MQ=60)

                                                                               GAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGT                >  SRR4302545.7053529/1‑94 (MQ=60)

                                                                               GAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGT                <  SRR4302545.8118893/94‑1 (MQ=60)

                                                                               GAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGT                <  SRR4302545.3316290/94‑1 (MQ=60)

                                                                                AATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTA               >  SRR4302545.2885234/1‑94 (MQ=60)

                                                                                 ATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAA              <  SRR4302545.10014654/94‑1 (MQ=60)

                                                                                 ATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAA              <  SRR4302545.10014653/94‑1 (MQ=60)

                                                                                 ATGTAATCTGGCGATTTTCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGACACACGCCCTATTTCTGTAA              <  SRR4302545.3574239/94‑1 (MQ=60)

                                                                                  TGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTCCTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAAC             <  SRR4302545.7925054/94‑1 (MQ=60)

                                                                                   GTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACG            <  SRR4302545.6841035/94‑1 (MQ=60)

                                                                                   GTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACG            >  SRR4302545.6192887/1‑94 (MQ=60)

                                                                                   GTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACG            <  SRR4302545.9840722/94‑1 (MQ=60)

                                                                                    TAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGC           <  SRR4302545.4209877/94‑1 (MQ=60)

                                                                                    TAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGC           >  SRR4302545.3603696/1‑94 (MQ=60)

                                                                                     AATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCC          >  SRR4302545.3290606/1‑94 (MQ=60)

                                                                                       TCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCT        <  SRR4302545.5973590/94‑1 (MQ=60)

                                                                                       TCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCT        <  SRR4302545.3437976/94‑1 (MQ=60)

                                                                                       TCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCT        <  SRR4302545.1735843/94‑1 (MQ=60)

                                                                                       TCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCT        <  SRR4302545.5533553/94‑1 (MQ=60)

                                                                                        CTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCTG       >  SRR4302545.1726286/1‑94 (MQ=60)

                                                                                        CTGGCGCTATCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCTG       <  SRR4302545.9653044/94‑1 (MQ=60)

                                                                                         TGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCTGG      <  SRR4302545.7109021/94‑1 (MQ=60)

                                                                                          GGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCTGGA     <  SRR4302545.3956037/94‑1 (MQ=60)

                                                                                          GGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCTGGA     <  SRR4302545.716302/94‑1 (MQ=60)

                                                                                          GGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACCGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCTGGA     <  SRR4302545.4825012/94‑1 (MQ=60)

                                                                                            CGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCTGGAAA   <  SRR4302545.5222988/94‑1 (MQ=60)

                                                                                            CGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCTGGAAA   <  SRR4302545.5222989/94‑1 (MQ=60)

                                                                                            CGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCTGGAAA   <  SRR4302545.646356/94‑1 (MQ=60)

                                                                                             GCTTTGCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCTGGAAAT  <  SRR4302545.3724365/94‑1 (MQ=60)

                                                                                             GCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCTGGAAAT  >  SRR4302545.3868083/1‑94 (MQ=60)

                                                                                             GCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCTGGAAAT  <  SRR4302545.9001774/94‑1 (MQ=60)

GGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCACTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCTGGAAAT  >  NC_000913/159355‑159541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`