Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	159,448	A→G	V222A (GTG→GCG)	yadB ←	putative tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	159,448	0	A	G	100.0%	787.6 / NA	229	V222A (GTG→GCG)	yadB	putative tRNA synthetase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (106/123); total (106/123)

Only 100 of 244 total aligned reads displayed.

TCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCACTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCTGG  >  NC_000913/159358‑159537

 cGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCtt                                                                                        <  1:5368728/93‑1 (MQ=255)

   ccTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTc                                                                                      <  1:853605/93‑1 (MQ=255)

   ccTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTc                                                                                      >  1:6161827/1‑93 (MQ=255)

   ccTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTc                                                                                      <  1:4077910/93‑1 (MQ=255)

    cTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTcc                                                                                     <  2:4963712/93‑1 (MQ=255)

    cTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTcc                                                                                     >  2:3359717/1‑93 (MQ=255)

    cTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTcc                                                                                     >  1:3863833/1‑93 (MQ=255)

    cTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTcc                                                                                     <  1:2994208/93‑1 (MQ=255)

     tttCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCa                                                                                    >  1:1529627/1‑93 (MQ=255)

     tttCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCa                                                                                    >  1:3080622/1‑93 (MQ=255)

     tttCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCa                                                                                    <  1:3290924/93‑1 (MQ=255)

        cGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGcc                                                                                 >  2:552202/1‑93 (MQ=255)

         ggCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCa                                                                                <  2:3792379/93‑1 (MQ=255)

          gCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCaa                                                                               >  2:3403617/1‑93 (MQ=255)

           cAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCaaa                                                                              <  2:2242016/93‑1 (MQ=255)

            aaCGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCaaaa                                                                             <  1:4902982/93‑1 (MQ=255)

             aCGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCaaaaa                                                                            >  1:5802452/1‑93 (MQ=255)

             aCGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCaaaaa                                                                            <  1:345884/93‑1 (MQ=255)

             aCGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATATGGCGCTTTCCAGCCaaaag                                                                            >  2:1809978/1‑92 (MQ=255)

               gCAGGCGCATGATTCTGCTTGAAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGc                                                                          <  2:5597206/93‑1 (MQ=255)

                 aGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTg                                                                        <  2:4654836/93‑1 (MQ=255)

                  ggCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTgg                                                                       <  2:6461278/93‑1 (MQ=255)

                    cgcATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTa                                                                     <  1:1683773/93‑1 (MQ=255)

                       aTGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAg                                                                  >  2:2858189/1‑93 (MQ=255)

                         gATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCg                                                                >  2:5122262/1‑93 (MQ=255)

                         gATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCg                                                                >  2:2308355/1‑93 (MQ=255)

                         gATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCg                                                                >  1:978726/1‑93 (MQ=255)

                          aTTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCgg                                                               >  1:2398109/1‑92 (MQ=255)

                           ttCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCgag                                                              >  1:63963/1‑93 (MQ=255)

                           ttCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCgag                                                              >  1:4947290/1‑93 (MQ=255)

                            tCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCgaga                                                             <  2:2013961/93‑1 (MQ=255)

                             cTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGAt                                                            <  1:136406/93‑1 (MQ=255)

                             cTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGAt                                                            <  1:4457337/93‑1 (MQ=255)

                                cTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTg                                                         <  1:781204/93‑1 (MQ=255)

                                 ttGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGc                                                        <  2:3139595/93‑1 (MQ=255)

                                  tGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGcc                                                       >  1:2248224/1‑93 (MQ=255)

                                  tGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGcc                                                       <  1:417652/93‑1 (MQ=255)

                                   ggAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCt                                                      <  2:1591594/93‑1 (MQ=255)

                                   ggAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCt                                                      >  2:6363838/1‑93 (MQ=255)

                                    gAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCtt                                                     <  1:5754658/93‑1 (MQ=255)

                                    gAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCtt                                                     >  2:5811293/1‑93 (MQ=255)

                                     aaaGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTa                                                    <  2:4522172/93‑1 (MQ=255)

                                      aaGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTAc                                                   >  2:4474285/1‑93 (MQ=255)

                                      aaGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTAc                                                   <  1:5664794/93‑1 (MQ=255)

                                       aGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACt                                                  <  1:2690125/93‑1 (MQ=255)

                                       aGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACt                                                  <  2:2833526/93‑1 (MQ=255)

                                        gTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTg                                                 <  2:811374/93‑1 (MQ=255)

                                         tttAGGGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGATTTCCAGCAAAAAAGCTGGTCCAGCGAGATTTGCCTTACTGt                                                <  2:841657/93‑1 (MQ=255)

                                         tttAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGt                                                <  1:3425231/93‑1 (MQ=255)

                                          ttggcgcGTTGGGAATAAAGCGCCGGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGtt                                               <  2:5049029/90‑1 (MQ=255)

                                          ttAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGtt                                               <  2:3849133/93‑1 (MQ=255)

                                           tAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTg                                              >  2:2093937/1‑93 (MQ=255)

                                           tAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTGGCCTTACTGTTg                                              <  2:4235479/93‑1 (MQ=255)

                                                ccTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCa                                         >  1:5096529/1‑93 (MQ=255)

                                                 cTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCaa                                        <  1:4629514/93‑1 (MQ=255)

                                                  ttGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAACAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAat                                       >  2:1426788/1‑93 (MQ=255)

                                                  ttGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAat                                       >  1:2325282/1‑93 (MQ=255)

                                                   tgtgGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAatc                                      <  1:3596413/93‑1 (MQ=255)

                                                    gtgGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAatca                                     >  1:660257/1‑93 (MQ=255)

                                                    gtgGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAatca                                     <  2:61572/93‑1 (MQ=255)

                                                     tgGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTTCAGACAAAAAGCTGGTACAGCGAGAGTTGCCTTACTGATGGTTCAatcag                                    >  2:3301554/1‑93 (MQ=255)

                                                     tgGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAatcag                                    <  1:3518331/93‑1 (MQ=255)

                                                      ggATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAatcaga                                   <  1:1102777/93‑1 (MQ=255)

                                                      ggATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAatcaga                                   <  1:1946028/93‑1 (MQ=255)

                                                       gATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAatcagat                                  >  1:4647645/1‑93 (MQ=255)

                                                        aTTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAatcagatc                                 <  1:5547869/93‑1 (MQ=255)

                                                        aTTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAatcagatc                                 <  2:2610489/93‑1 (MQ=255)

                                                        aTTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAatcagatc                                 <  2:4110227/93‑1 (MQ=255)

                                                         ttAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAatcagatca                                >  1:3878781/1‑93 (MQ=255)

                                                          tAAACGCCAGCGACAGATGAATGTAACCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAatcagatcag                               <  2:1169018/93‑1 (MQ=255)

                                                             gcgcCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGccc                            <  2:6252176/93‑1 (MQ=255)

                                                             gcgcCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGccc                            <  1:6283021/93‑1 (MQ=255)

                                                                 cAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACg                        <  2:1173450/93‑1 (MQ=255)

                                                                     ggCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACt                    >  1:3772344/1‑93 (MQ=255)

                                                                      gCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTa                   <  2:1087301/93‑1 (MQ=255)

                                                                      gCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTa                   >  2:192338/1‑93 (MQ=255)

                                                                      gCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTa                   <  1:2017180/93‑1 (MQ=255)

                                                                       cAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTAt                  <  2:2483981/93‑1 (MQ=255)

                                                                        aGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTAtt                 >  1:6107174/1‑93 (MQ=255)

                                                                         gATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTAttt                >  1:3673255/1‑93 (MQ=255)

                                                                          aTGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTc               <  2:566066/93‑1 (MQ=255)

                                                                           tGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCt              >  2:3972906/1‑93 (MQ=255)

                                                                            gAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTg             >  1:5858700/1‑93 (MQ=255)

                                                                             aaTGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGt            <  2:1495188/93‑1 (MQ=255)

                                                                             aaTGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGt            >  2:5169469/1‑93 (MQ=255)

                                                                               tGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTaa          <  1:3273191/93‑1 (MQ=255)

                                                                               tGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTaa          >  2:3513878/1‑93 (MQ=255)

                                                                                gTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAAc         >  1:4424574/1‑93 (MQ=255)

                                                                                gTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAAc         <  2:2406310/93‑1 (MQ=255)

                                                                                 tAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACg        <  1:4247/93‑1 (MQ=255)

                                                                                  aaTCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGc       >  2:5859300/1‑93 (MQ=255)

                                                                                   aTCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGcc      >  2:2940175/1‑93 (MQ=255)

                                                                                   aTCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGcc      <  2:5068897/93‑1 (MQ=255)

                                                                                   aTCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGcc      <  1:3391433/93‑1 (MQ=255)

                                                                                   aTCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGcc      >  1:1182900/1‑93 (MQ=255)

                                                                                    tCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGccc     <  2:1806471/93‑1 (MQ=255)

                                                                                    tCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGccc     <  1:2602428/93‑1 (MQ=255)

                                                                                     cTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCt    <  2:5536726/93‑1 (MQ=255)

                                                                                     cTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCt    >  2:4829095/1‑93 (MQ=255)

                                                                                      tGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCTg   <  2:5477718/93‑1 (MQ=255)

TCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCACTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCTGG  >  NC_000913/159358‑159537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCACTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCTGGAAAT  >  NC_000913/159358‑159541

TCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTT                                                                                            >  SRR4302537.2073493/1‑94 (MQ=60)

TCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTT                                                                                            <  SRR4302537.580066/94‑1 (MQ=60)

 CGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTT                                                                                           >  SRR4302537.698047/1‑94 (MQ=60)

 CGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTT                                                                                           <  SRR4302537.5405462/94‑1 (MQ=60)

  GCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTC                                                                                          >  SRR4302537.3577900/1‑94 (MQ=60)

  GCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTC                                                                                          >  SRR4302537.6204231/1‑94 (MQ=60)

  GCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTC                                                                                          >  SRR4302537.3154525/1‑94 (MQ=60)

  GCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTC                                                                                          >  SRR4302537.5837674/1‑94 (MQ=60)

  GCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTC                                                                                          <  SRR4302537.1763773/94‑1 (MQ=60)

   CCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTTAGCGCC‑GCAGCAGATGGATGTAATCTGGCGCGTTCCA                                                                                        >  SRR4302537.139770/1‑94 (MQ=60)

   CCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCC                                                                                         >  SRR4302537.3890882/1‑94 (MQ=60)

   CCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCC                                                                                         <  SRR4302537.4106381/94‑1 (MQ=60)

   CCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCC                                                                                         <  SRR4302537.859461/94‑1 (MQ=60)

    CTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCA                                                                                        >  SRR4302537.1540317/1‑94 (MQ=60)

    CTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCA                                                                                        <  SRR4302537.3333042/94‑1 (MQ=60)

    CTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCA                                                                                        <  SRR4302537.3348550/94‑1 (MQ=60)

    CTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCA                                                                                        >  SRR4302537.3102076/1‑94 (MQ=60)

    CTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCA                                                                                        <  SRR4302537.3015095/94‑1 (MQ=60)

     TTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAG                                                                                       <  SRR4302537.3314178/94‑1 (MQ=60)

       TCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCC                                                                                     <  SRR4302537.4483013/94‑1 (MQ=60)

            AACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAA                                                                                <  SRR4302537.4936824/94‑1 (MQ=60)

            AACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAA                                                                                >  SRR4302537.5842234/1‑94 (MQ=60)

             ACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAG                                                                               <  SRR4302537.348289/94‑1 (MQ=60)

             ACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAG                                                                               >  SRR4302537.2447084/1‑94 (MQ=60)

                 AGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGG                                                                           >  SRR4302537.3914225/1‑94 (MQ=60)

                    CGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTAC                                                                        <  SRR4302537.1695559/94‑1 (MQ=60)

                      CATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAG                                                                      >  SRR4302537.4376338/1‑94 (MQ=60)

                       ATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGGTTTCCAGCCAAAAAGCTGGTACAGG                                                                     >  SRR4302537.6428522/1‑94 (MQ=60)

                       ATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGC                                                                     >  SRR4302537.873075/1‑94 (MQ=60)

                        TGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCG                                                                    >  SRR4302537.985482/1‑94 (MQ=60)

                         GATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGG                                                                   >  SRR4302537.2414999/1‑94 (MQ=60)

                         GATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGA                                                                   >  SRR4302537.2671923/1‑94 (MQ=60)

                         GATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGA                                                                   >  SRR4302537.597274/1‑94 (MQ=60)

                          ATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAG                                                                  >  SRR4302537.64438/1‑94 (MQ=60)

                          ATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAG                                                                  >  SRR4302537.4981398/1‑94 (MQ=60)

                          ATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAG                                                                  >  SRR4302537.2247303/1‑94 (MQ=60)

                           TTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGA                                                                 >  SRR4302537.3999101/1‑94 (MQ=60)

                            TCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGAT                                                                <  SRR4302537.3504947/94‑1 (MQ=60)

                             CTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATT                                                               <  SRR4302537.4488121/94‑1 (MQ=60)

                             CTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATT                                                               <  SRR4302537.137381/94‑1 (MQ=60)

                              TGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTT                                                              <  SRR4302537.5165106/94‑1 (MQ=60)

                              TGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTT                                                              <  SRR4302537.5165105/94‑1 (MQ=60)

                              TGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTT                                                              <  SRR4302537.78931/94‑1 (MQ=60)

                                CTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGC                                                            <  SRR4302537.786570/94‑1 (MQ=60)

                                 TTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCC                                                           >  SRR4302537.2264089/1‑94 (MQ=60)

                                  TGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCT                                                          <  SRR4302537.420552/94‑1 (MQ=60)

                                  TGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCT                                                          <  SRR4302537.4631817/94‑1 (MQ=60)

                                  TGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCT                                                          <  SRR4302537.1981471/94‑1 (MQ=60)

                                    GAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTA                                                        <  SRR4302537.1795219/94‑1 (MQ=60)

                                    GAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTA                                                        <  SRR4302537.5794073/94‑1 (MQ=60)

                                     AAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTAC                                                       >  SRR4302537.2290651/1‑94 (MQ=60)

                                      AAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACT                                                      <  SRR4302537.3303138/94‑1 (MQ=60)

                                      AAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACT                                                      <  SRR4302537.5703550/94‑1 (MQ=60)

                                       AGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTG                                                     <  SRR4302537.2708898/94‑1 (MQ=60)

                                         TTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTT                                                   >  SRR4302537.6364717/1‑94 (MQ=60)

                                         TTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTT                                                   <  SRR4302537.3449476/94‑1 (MQ=60)

                                            AGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGT                                                >  SRR4302537.3770547/1‑94 (MQ=60)

                                               GCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCA                                             >  SRR4302537.5131625/1‑94 (MQ=60)

                                                CCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAA                                            >  SRR4302537.3423426/1‑94 (MQ=60)

                                                 CTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAAT                                           >  SRR4302537.2341692/1‑94 (MQ=60)

                                                 CTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAAT                                           <  SRR4302537.4661543/94‑1 (MQ=60)

                                                 CTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAAT                                           <  SRR4302537.567024/94‑1 (MQ=60)

                                                   TGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCA                                         >  SRR4302537.605668/1‑94 (MQ=60)

                                                   TGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCA                                         <  SRR4302537.3621765/94‑1 (MQ=60)

                                                   TGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCA                                         >  SRR4302537.664754/1‑94 (MQ=60)

                                                     TGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGA                                       <  SRR4302537.4341627/94‑1 (MQ=60)

                                                     TGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGA                                       <  SRR4302537.3543224/94‑1 (MQ=60)

                                                      GGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGAT                                      <  SRR4302537.5014417/94‑1 (MQ=60)

                                                      GGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGAT                                      >  SRR4302537.5596617/1‑94 (MQ=60)

                                                      GGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGAT                                      <  SRR4302537.1959558/94‑1 (MQ=60)

                                                      GGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGAT                                      >  SRR4302537.4679777/1‑94 (MQ=60)

                                                      GGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGAT                                      <  SRR4302537.1110396/94‑1 (MQ=60)

                                                      GGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGAT                                      <  SRR4302537.3900650/94‑1 (MQ=60)

                                                       GATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATC                                     >  SRR4302537.2955004/1‑94 (MQ=60)

                                                        ATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCA                                    <  SRR4302537.5585749/94‑1 (MQ=60)

                                                        ATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCA                                    <  SRR4302537.3675281/94‑1 (MQ=60)

                                                        ATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCA                                    >  SRR4302537.3905908/1‑94 (MQ=60)

                                                        ATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCA                                    >  SRR4302537.3167787/1‑94 (MQ=60)

                                                        ATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCA                                    >  SRR4302537.1123338/1‑94 (MQ=60)

                                                         TTAAGCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAG                                   <  SRR4302537.38763/94‑1 (MQ=60)

                                                             GCGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCC                               <  SRR4302537.6326105/94‑1 (MQ=60)

                                                              CGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCA                              <  SRR4302537.4760213/94‑1 (MQ=60)

                                                              CGCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCA                              >  SRR4302537.619719/1‑94 (MQ=60)

                                                               GCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCAC                             >  SRR4302537.4255658/1‑94 (MQ=60)

                                                               GCCAGCGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCAC                             <  SRR4302537.783455/94‑1 (MQ=60)

                                                                 CAGCGGCAGATGAGTGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGC                           <  SRR4302537.6486110/94‑1 (MQ=60)

                                                                    CGGCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACT                        >  SRR4302537.3798827/1‑94 (MQ=60)

                                                                      GCAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTAT                      <  SRR4302537.2031242/94‑1 (MQ=60)

                                                                       CAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATT                     >  SRR4302537.6149134/1‑94 (MQ=60)

                                                                       CAGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATT                     >  SRR4302537.1556296/1‑94 (MQ=60)

                                                                        AGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTT                    >  SRR4302537.3699097/1‑94 (MQ=60)

                                                                        AGATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTT                    <  SRR4302537.1521573/94‑1 (MQ=60)

                                                                         GATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTC                   <  SRR4302537.3806210/94‑1 (MQ=60)

                                                                          ATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCT                  <  SRR4302537.1274497/94‑1 (MQ=60)

                                                                          ATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCT                  >  SRR4302537.2872223/1‑94 (MQ=60)

                                                                          ATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCT                  <  SRR4302537.4739849/94‑1 (MQ=60)

                                                                          ATGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCT                  <  SRR4302537.2501422/94‑1 (MQ=60)

                                                                          AGGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCT                  <  SRR4302537.3524825/94‑1 (MQ=60)

                                                                           TGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTG                 >  SRR4302537.3481748/1‑94 (MQ=60)

                                                                           TGAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTG                 >  SRR4302537.5898883/1‑94 (MQ=60)

                                                                            GAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGT                <  SRR4302537.5483748/94‑1 (MQ=60)

                                                                            GAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGT                >  SRR4302537.961159/1‑94 (MQ=60)

                                                                            GAATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGT                >  SRR4302537.3052524/1‑94 (MQ=60)

                                                                             AATGTAATCTGGCGCTTTCCAGCCAAAAGGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTA               >  SRR4302537.5898014/1‑94 (MQ=60)

                                                                             AATGTAATCGGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTA               <  SRR4302537.2362992/94‑1 (MQ=60)

                                                                              ATGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAA              >  SRR4302537.5717254/1‑94 (MQ=60)

                                                                               TGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAAC             >  SRR4302537.4455131/1‑94 (MQ=60)

                                                                               TGTAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAAC             <  SRR4302537.3296289/94‑1 (MQ=60)

                                                                                 TAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGC           <  SRR4302537.4282/94‑1 (MQ=60)

                                                                                 TAATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGC           >  SRR4302537.3630560/1‑94 (MQ=60)

                                                                                  AATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCC          >  SRR4302537.1191126/1‑94 (MQ=60)

                                                                                  AATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCC          >  SRR4302537.6264052/1‑94 (MQ=60)

                                                                                   ATCTGGCGCTTTCCAGCCAAAAAGCTTGTACAGCGAGATTTGCCTTTCTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCC         <  SRR4302537.4948527/94‑1 (MQ=60)

                                                                                   ATCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCC         <  SRR4302537.3415456/94‑1 (MQ=60)

                                                                                    TCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCT        <  SRR4302537.4559894/94‑1 (MQ=60)

                                                                                    TCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCT        <  SRR4302537.4677727/94‑1 (MQ=60)

                                                                                    TCTGGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCT        <  SRR4302537.2620623/94‑1 (MQ=60)

                                                                                    TCTGCCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCT        <  SRR4302537.4111209/94‑1 (MQ=60)

                                                                                       GGCGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCTGGA     >  SRR4302537.1174194/1‑94 (MQ=60)

                                                                                         CGCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTTTTACGCCCTGGAAA   >  SRR4302537.1331590/1‑94 (MQ=60)

                                                                                          GCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCTGGAAAT  <  SRR4302537.4934376/94‑1 (MQ=60)

                                                                                          GCTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCTGGAAAT  <  SRR4302537.4440988/94‑1 (MQ=60)

TCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCACTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCTGGAAAT  >  NC_000913/159358‑159541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`