Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	647	270.1	21258240	98.4%	20918108	94.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	176,432	A→G	K442K (AAA→AAG)	yadQ →	putative channel transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	176,432	0	A	G	97.8%	853.3 / 3.4	278	K442K (AAA→AAG)	yadQ	putative channel transporter
Reads supporting (aligned to +/- strand): ref base A (3/3); new base G (123/149); total (126/152)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-01

Only 100 of 348 total aligned reads displayed.

TTCTGGAGATGACCGATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAACCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCAGCGAGAAT  >  NC_000913/176341‑176522

ttctggAGATGACCGATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGcc                                                                                          <  1:1015257/94‑1 (MQ=255)

ttctggAGATGACCGATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGcc                                                                                          <  2:2947617/94‑1 (MQ=255)

 tctggAGATGACCGATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCg                                                                                         <  1:9886717/94‑1 (MQ=255)

  ctggAGATGACCGATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGc                                                                                        <  2:3696689/94‑1 (MQ=255)

   tggAGATGACCGATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCt                                                                                       >  2:430506/1‑94 (MQ=255)

      agagGACCGATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCtata                                                                                    <  2:3390496/94‑1 (MQ=255)

      agaTGACCGATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGGAGCCGCtata                                                                                    >  1:2416571/1‑94 (MQ=255)

        aTGACCGATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACt                                                                                  <  2:19776/94‑1 (MQ=255)

        aTGACCGATAACTACCAGCTCATTTCGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGGCGCTATACt                                                                                  >  2:9698620/1‑94 (MQ=255)

          tcccGATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAACCGCTATACTCg                                                                                <  1:5653584/92‑1 (MQ=255)

          gACCGATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCg                                                                                <  2:3665962/94‑1 (MQ=255)

           aCCGATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGGAGCCGCGATACTCgg                                                                               >  1:7314324/1‑94 (MQ=255)

            ccGATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGc                                                                              >  2:1440611/1‑94 (MQ=255)

            ccGATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGc                                                                              <  1:6772341/94‑1 (MQ=255)

            ccGATAAATACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGACGACATACTAGGc                                                                              >  2:4915268/1‑94 (MQ=255)

              gATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCgt                                                                            >  2:5678958/1‑93 (MQ=255)

                 aaCTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTc                                                                         <  2:4165593/94‑1 (MQ=255)

                  aCTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCTCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCt                                                                        <  1:1151217/94‑1 (MQ=255)

                  aCTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCt                                                                        <  1:9215234/94‑1 (MQ=255)

                   cTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTTGCGCAACACTATTAGCGCAATTTACCGGCGGGTAAACCCCCTAGTCGGCCAtttct                                                                       >  2:9782775/1‑91 (MQ=255)

                   cTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCtt                                                                       >  1:2018341/1‑94 (MQ=255)

                    tACCATCTCATTTTGCCAATTATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTg                                                                      <  1:5299585/94‑1 (MQ=255)

                     aCCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTgc                                                                     <  2:2518040/94‑1 (MQ=255)

                      ccAGCTCATTTTGCCAATGATTATTGCCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTgcg                                                                    >  1:8993955/1‑94 (MQ=255)

                      ccAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGGGATTCTTgcg                                                                    >  1:6211793/1‑94 (MQ=255)

                      ccAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTgcg                                                                    >  1:8088439/1‑94 (MQ=255)

                         gCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTgcgcgc                                                                 <  1:9455757/94‑1 (MQ=255)

                          cTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCa                                                                >  2:737773/1‑94 (MQ=255)

                          cTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCa                                                                >  2:5468853/1‑94 (MQ=255)

                           tCATTTTTCCAATGATTATTGCCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCAc                                                               <  1:36153/94‑1 (MQ=255)

                           tCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCAc                                                               >  2:9202090/1‑94 (MQ=255)

                           tCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCAc                                                               <  1:1752498/94‑1 (MQ=255)

                           tCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCAc                                                               <  1:9683532/94‑1 (MQ=255)

                            cATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACg                                                              >  1:2892927/1‑94 (MQ=255)

                             aTTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGc                                                             >  2:7122187/1‑94 (MQ=255)

                             aTTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGc                                                             >  2:7772732/1‑94 (MQ=255)

                              ttttGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCt                                                            <  1:9332147/94‑1 (MQ=255)

                               tttGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATTACCGGCGATTCTTGCGCGCACGCTg                                                           >  2:8459967/1‑94 (MQ=255)

                                ttGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTgg                                                          <  2:6581975/94‑1 (MQ=255)

                                ttGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTgg                                                          <  2:6970156/94‑1 (MQ=255)

                                   ccAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCCCGCTGGCaa                                                       >  1:459111/1‑94 (MQ=255)

                                   ccAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCCGAGATTCTTGCGCGCACGCCGGcca                                                       >  1:5837241/1‑92 (MQ=255)

                                     aaTGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCaaaa                                                     <  2:5359734/94‑1 (MQ=255)

                                      aTGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGGGCGCACGCTGGCaaaaa                                                    >  1:7007295/1‑93 (MQ=255)

                                      aTGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAAc                                                    <  2:1316144/94‑1 (MQ=255)

                                      aTGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAAc                                                    <  2:9910072/94‑1 (MQ=255)

                                         attattACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAgg                                                 >  1:5390896/1‑94 (MQ=255)

                                         attattACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAgg                                                 <  1:6748910/94‑1 (MQ=255)

                                          ttattaCCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGa                                                <  2:5836228/94‑1 (MQ=255)

                                          ttattaCCGGTCTTGGAGCAACACTATTAGCGCAATTTACAGGCGGGGAGGCGCTATACTCGGCGATTCTTGGGCGCGCGCTGGCCAAACATGa                                                >  1:5106323/1‑94 (MQ=255)

                                            attaCCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGGAGCCGCTATACTCGGCGATTCCTGCGCGCCACCTGGCAAAACCGGAAg                                              >  2:10229979/1‑94 (MQ=255)

                                            attaCCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAg                                              >  1:8171061/1‑94 (MQ=255)

                                             ttaCCGGTCTTGTCGCAACACTATTAGCGCAATTTCCCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGc                                             <  1:4787081/94‑1 (MQ=255)

                                              taCCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTTCGCGCGCGCTGGCCAAACAGGAAGCt                                            >  2:9069742/1‑94 (MQ=255)

                                               aCCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTg                                           >  2:1061023/1‑94 (MQ=255)

                                               aCCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTg                                           <  1:4083176/94‑1 (MQ=255)

                                                ccGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTTCGCGCACGCTGGCAAAACAGGAAGCTGa                                          >  2:4574384/1‑94 (MQ=255)

                                                     cTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAAc                                     <  2:10557635/94‑1 (MQ=255)

                                                      ttGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAAAAGGAAGCCGAGCaaat                                    >  1:5959894/1‑92 (MQ=255)

                                                       tGGCGCAAACCTATTAGCGCAATGTACCGGCGGGAAGCCGCTATACTCGGCGAGTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTg                                   <  2:8163122/94‑1 (MQ=255)

                                                           gcAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGgcgc                               <  1:6900206/94‑1 (MQ=255)

                                                            cAACACTATTAGCGCAATTTACCGGCGGGGAGCTGCTATACTCGGCGAGTTTTGCGCGCACGCCGTCAAAATACGAAGCCTAGCAACCGGcgcg                              >  2:9385582/1‑94 (MQ=255)

                                                            cAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGGGATTCTTGCGCGCACGCTGGGAAAACAGAAAGCTGAGAAACTGGcgcg                              >  1:3791205/1‑94 (MQ=255)

                                                            cAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGcgcg                              >  2:7178857/1‑94 (MQ=255)

                                                             aaCACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGa                             <  2:5072234/94‑1 (MQ=255)

                                                             aaCACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGa                             >  2:2628006/1‑94 (MQ=255)

                                                              acacTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGaa                            >  1:4671987/1‑94 (MQ=255)

                                                               cacTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAg                           <  1:841156/94‑1 (MQ=255)

                                                               cacTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACCGGCGCGAAg                           >  1:2118888/1‑94 (MQ=255)

                                                                acTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGc                          >  2:7564473/1‑94 (MQ=255)

                                                                acTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACCGGCGCGAAGc                          >  1:4397194/1‑94 (MQ=255)

                                                                 cTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCa                         <  1:340164/94‑1 (MQ=255)

                                                                   aTTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAg                       <  2:29120/94‑1 (MQ=255)

                                                                    ttAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAgg                      <  2:8842376/94‑1 (MQ=255)

                                                                      aGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGcc                    <  1:6360230/94‑1 (MQ=255)

                                                                      aGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGcc                    <  2:9472464/94‑1 (MQ=255)

                                                                      aGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGcc                    <  1:7542641/94‑1 (MQ=255)

                                                                        cgcAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCGAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGgcgc                  >  2:4502916/1‑94 (MQ=255)

                                                                         gcAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGACGAACTGGCGCGAAGCAAGGccccc                 >  1:8852810/1‑91 (MQ=255)

                                                                          cAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCAt                >  1:6892089/1‑94 (MQ=255)

                                                                            aTTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATca              <  1:1458755/94‑1 (MQ=255)

                                                                              ttACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATcagc            <  1:7821859/94‑1 (MQ=255)

                                                                               tACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATcagcc           <  1:5005389/94‑1 (MQ=255)

                                                                                aCCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATcagccc          >  1:3009531/1‑93 (MQ=255)

                                                                                aCCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATcagcca          <  1:8536423/94‑1 (MQ=255)

                                                                                aCCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATcagcca          <  2:321075/94‑1 (MQ=255)

                                                                                 ccGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATcagccag         <  2:6011150/94‑1 (MQ=255)

                                                                                   ggcggGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCAGCg       >  2:9590140/1‑94 (MQ=255)

                                                                                    gcggGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCAGCga      <  2:6731138/94‑1 (MQ=255)

                                                                                    gcggGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCAGCga      >  1:4226542/1‑94 (MQ=255)

                                                                                    gcggGAAACCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATAAGGCAGCgg      >  1:5547079/1‑93 (MQ=255)

                                                                                     tgggAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGaa                                               >  1:3151085/2‑52 (MQ=255)

                                                                                     cggGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCAGCgag     <  2:1855901/94‑1 (MQ=255)

                                                                                     cggGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCAGCgag     >  1:1346623/1‑94 (MQ=255)

                                                                                      gggAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCAGCgaga    >  2:635780/1‑94 (MQ=255)

                                                                                       ggAAGCCGCTATACTCGGCGATTCTTGCGCGCATGCTGGCAAAACAGGAAGCTGAGCAACAGGCGCGAAGCAAGGCCGCATCAGCCAGCGAGaa   <  1:6048763/94‑1 (MQ=255)

                                                                                        gAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCAGCGAGAAt  <  1:2762696/94‑1 (MQ=255)

                                                                                        gAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCAGCGAGAAt  <  1:2605011/94‑1 (MQ=255)

                                                                                        gAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCAGCGAGAAt  <  2:161187/94‑1 (MQ=255)

                                                                                        gAAACCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCAGCGAGAAt  <  1:10429705/94‑1 (MQ=255)

TTCTGGAGATGACCGATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAACCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCAGCGAGAAT  >  NC_000913/176341‑176522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 205 total aligned reads displayed.

GGTTCTGGAGATGACCGATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAACCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCAGCGAGAATACT  >  NC_000913/176339‑176525

GGTTCTGGAGATGACCGATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAG                                                                                               >  SRR4302545.2272403/1‑94 (MQ=60)

GGTTCTGGAGATGACCGATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAG                                                                                               <  SRR4302545.4126535/94‑1 (MQ=60)

  TTCTGGAGATGACCGATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCC                                                                                             <  SRR4302545.1037653/94‑1 (MQ=60)

   TGTGGAGATGACCGATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCG                                                                                            <  SRR4302545.10586252/94‑1 (MQ=60)

   TCTGGAGATGACCGATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCG                                                                                            <  SRR4302545.10180476/94‑1 (MQ=60)

    CTGGAGATGACCGATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGC                                                                                           <  SRR4302545.2658857/94‑1 (MQ=60)

    CTGGAGATGACCGATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGC                                                                                           >  SRR4302545.4152511/1‑94 (MQ=60)

     TGGAGATGACCGATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCT                                                                                          <  SRR4302545.7671199/94‑1 (MQ=60)

     TGGAGATGACCGATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCT                                                                                          <  SRR4302545.10117315/94‑1 (MQ=60)

      GGAGATGACCGATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTA                                                                                         >  SRR4302545.3858846/1‑94 (MQ=60)

       GAGATGACCGATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTAT                                                                                        <  SRR4302545.2390874/94‑1 (MQ=60)

        AGATGACCGATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGGAGCCGCTATA                                                                                       >  SRR4302545.2475127/1‑94 (MQ=60)

          ATGACCGATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACT                                                                                     <  SRR4302545.5553237/94‑1 (MQ=60)

           TGACCGATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTC                                                                                    <  SRR4302545.6091966/94‑1 (MQ=60)

           TGACCGATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTC                                                                                    >  SRR4302545.8350492/1‑94 (MQ=60)

            tcCCGATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAACCGCTATACTCG                                                                                   <  SRR4302545.5804241/92‑1 (MQ=60)

            GACCGATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTCTACTCG                                                                                   >  SRR4302545.6140018/1‑94 (MQ=60)

            GACCGATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCG                                                                                   <  SRR4302545.8721920/94‑1 (MQ=60)

             ACCGATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGGAGCCGCGATACTCGG                                                                                  >  SRR4302545.7519465/1‑94 (MQ=60)

              CCGATACCTCCCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGC                                                                                 <  SRR4302545.1523053/94‑1 (MQ=60)

              CCGATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCTCTATACTCGGC                                                                                 <  SRR4302545.6958111/94‑1 (MQ=60)

              CCGATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGC                                                                                 <  SRR4302545.6958112/94‑1 (MQ=60)

               CGATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGGAGCCGCTATACTCCGCG                                                                                >  SRR4302545.235392/1‑94 (MQ=60)

               CGATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCG                                                                                >  SRR4302545.906323/1‑94 (MQ=60)

               CGATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCG                                                                                <  SRR4302545.130520/94‑1 (MQ=60)

                GATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGGAGACGCGATACTCGGCGA                                                                               >  SRR4302545.5893163/1‑94 (MQ=60)

                GATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGA                                                                               >  SRR4302545.6702985/1‑94 (MQ=60)

                GATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATAATCGGCGA                                                                               >  SRR4302545.4985879/1‑94 (MQ=60)

                 ATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGAT                                                                              >  SRR4302545.2157778/1‑94 (MQ=60)

                  TAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAAGCGCTATTCTCGGgggtt                                                                             >  SRR4302545.6653780/1‑89 (MQ=60)

                   AACTACCAGCTCATTTTGCTAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTC                                                                            <  SRR4302545.5424306/94‑1 (MQ=60)

                    ACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCTCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCT                                                                           <  SRR4302545.1176469/94‑1 (MQ=60)

                    ACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCT                                                                           <  SRR4302545.9485382/94‑1 (MQ=60)

                    ACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCT                                                                           <  SRR4302545.9486276/94‑1 (MQ=60)

                    ACGACCAGCTCATTTTGCCAATGCTTCTTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATCCTCGGCGATTCT                                                                           <  SRR4302545.5203276/94‑1 (MQ=60)

                     CTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGGAGCCGCTATACTCGGCGGTTCTT                                                                          >  SRR4302545.8102836/1‑94 (MQ=60)

                     CTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTT                                                                          >  SRR4302545.2068183/1‑94 (MQ=60)

                     CTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTT                                                                          <  SRR4302545.501332/94‑1 (MQ=60)

                      TACCATCTCATTTTGCCAATTATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTG                                                                         <  SRR4302545.5436902/94‑1 (MQ=60)

                      TACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTG                                                                         <  SRR4302545.3424148/94‑1 (MQ=60)

                       ACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGC                                                                        <  SRR4302545.483424/94‑1 (MQ=60)

                        CCAGCTCATTTTGCCAATGATTATTGCCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGGAGACGCTATACTCCGCGATTTTTTCG                                                                       >  SRR4302545.9255814/1‑94 (MQ=60)

                        CCAGCTCATTTTGCCAATGATTATTGCCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCG                                                                       >  SRR4302545.9255815/1‑94 (MQ=60)

                        CCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTCTAATCAGCGATTCTTGCG                                                                       >  SRR4302545.6380885/1‑94 (MQ=60)

                        CCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGGGATTCTTGCG                                                                       >  SRR4302545.6380883/1‑94 (MQ=60)

                        CCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCG                                                                       >  SRR4302545.8321365/1‑94 (MQ=60)

                         CAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGGAGCCGCTATACTCGGCGATTCTTTCGC                                                                      >  SRR4302545.4480865/1‑94 (MQ=60)

                         CAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGC                                                                      <  SRR4302545.8481639/94‑1 (MQ=60)

                          AGATCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGACGCCATACTTGGCGATTCTTGCGCG                                                                     >  SRR4302545.6951185/1‑94 (MQ=60)

                           GCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGGGTCTTGCGCGC                                                                    >  SRR4302545.2168996/1‑94 (MQ=60)

                           GCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTTTTTCGCGC                                                                    >  SRR4302545.2068000/1‑94 (MQ=60)

                           GCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGC                                                                    <  SRR4302545.9733730/94‑1 (MQ=60)

                            CTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCC                                                                   >  SRR4302545.902439/1‑94 (MQ=60)

                             TCATTTTTCCAATGATTATTGCCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCAC                                                                  <  SRR4302545.36977/94‑1 (MQ=60)

                             TCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCAC                                                                  <  SRR4302545.9971069/94‑1 (MQ=60)

                             TCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCAC                                                                  >  SRR4302545.9720194/1‑94 (MQ=60)

                             TCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCAC                                                                  >  SRR4302545.1427453/1‑94 (MQ=60)

                             TCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCAC                                                                  <  SRR4302545.1794428/94‑1 (MQ=60)

                             TCATTTTGCCAATGATGATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTCCCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCAC                                                                  <  SRR4302545.6637718/94‑1 (MQ=60)

                              CATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACG                                                                 >  SRR4302545.2963025/1‑94 (MQ=60)

                              CATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACG                                                                 >  SRR4302545.2963024/1‑94 (MQ=60)

                              CATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACG                                                                 <  SRR4302545.967234/94‑1 (MQ=60)

                              CATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACG                                                                 <  SRR4302545.9322878/94‑1 (MQ=60)

                              CATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACG                                                                 >  SRR4302545.7748605/1‑94 (MQ=60)

                              CATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCCGCGATTCTTTCGCGCACC                                                                 >  SRR4302545.7968131/1‑94 (MQ=60)

                              CATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATAATCGGCGATTCTTGCGCGCACG                                                                 >  SRR4302545.3446432/1‑94 (MQ=60)

                                TTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCT                                                               <  SRR4302545.9605762/94‑1 (MQ=60)

                                 TTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGGAGCCGCTATACTAGGCGATTCTTGCGCGCACGCTG                                                              >  SRR4302545.5488916/1‑94 (MQ=60)

                                 TTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTG                                                              <  SRR4302545.4762419/94‑1 (MQ=60)

                                 TTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTG                                                              >  SRR4302545.599013/1‑94 (MQ=60)

                                  TTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGG                                                             >  SRR4302545.6140525/1‑94 (MQ=60)

                                  TTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGG                                                             <  SRR4302545.122231/94‑1 (MQ=60)

                                   tgccaataataaagACCGGTCTGGGCGCAACACTATTAACGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCACACGCTGGC                                                            <  SRR4302545.8965944/80‑1 (MQ=60)

                                   TGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGGAGGCGCTATAATCGGCGGTTTTTGCGCGCACGCTGGG                                                            >  SRR4302545.4072462/1‑94 (MQ=60)

                                     CCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGGCGCTATAATCCGCGATTTTTGCGCGCACGCTGGCAA                                                          >  SRR4302545.2854061/1‑94 (MQ=60)

                                     CCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCCCGCTGGCAA                                                          >  SRR4302545.469339/1‑94 (MQ=60)

                                     CCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCCGAGATTCTTGCGCGCACGCCGGCCA                                                          >  SRR4302545.5994387/1‑94 (MQ=60)

                                       AATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCGCGCTGGCAAAA                                                        >  SRR4302545.8724226/1‑94 (MQ=60)

                                       AATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAA                                                        <  SRR4302545.7366435/94‑1 (MQ=60)

                                       AATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAA                                                        <  SRR4302545.2979051/94‑1 (MQ=60)

                                        ATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTTGGCGGACGCTGGCAAAAC                                                       >  SRR4302545.7200631/1‑94 (MQ=60)

                                        ATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGGGCGCACGCTGGCAAAAA                                                       >  SRR4302545.7200632/1‑94 (MQ=60)

                                         TGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACA                                                      <  SRR4302545.6491840/94‑1 (MQ=60)

                                         TGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGACGCTCTAATCGGCGAGTCTTGCGCGCACGGTGGCGAGACA                                                      >  SRR4302545.4036079/1‑94 (MQ=60)

                                          ctcttccgatctctgatctGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAG                                                     <  SRR4302545.8563965/75‑1 (MQ=60)

                                          GATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCCCGCTGGCAAAACAG                                                     >  SRR4302545.819564/1‑94 (MQ=60)

                                           ATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGG                                                    >  SRR4302545.149762/1‑94 (MQ=60)

                                           ATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGG                                                    >  SRR4302545.7180699/1‑94 (MQ=60)

                                           ATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGG                                                    <  SRR4302545.6933983/94‑1 (MQ=60)

                                           ATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGG                                                    >  SRR4302545.5531331/1‑94 (MQ=60)

                                            TTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGGGGTTCTTTCGCGCGCGCTGGCAAAACAGGA                                                   >  SRR4302545.3211293/1‑94 (MQ=60)

                                            TTATTACCGGTCTTGGAGCAACACTATTAGCGCAATTTACAGGCGGGGAGGCGCTATACTCGGCGATTCTTGGGCGCGCGCTGGCCAAACATGA                                                   >  SRR4302545.5236861/1‑94 (MQ=57)

                                             TATTACCGGTCTTGGCGCAACCCTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACCCGGCGATTTTTGCGCGCCCGCTGGCAAAACAGGAA                                                  >  SRR4302545.7437564/1‑94 (MQ=60)

                                             TATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAA                                                  >  SRR4302545.435257/1‑94 (MQ=60)

                                              ATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCCCGCTGGCAAAACAGGAAG                                                 >  SRR4302545.5563026/1‑94 (MQ=60)

                                              ATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAG                                                 >  SRR4302545.8407346/1‑94 (MQ=60)

                                               TTACCGGTCTTGTCGCAACACTATTAGCGCAATTTCCCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGC                                                <  SRR4302545.4909478/94‑1 (MQ=60)

                                               TTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCCCGCTGGCAAAACAGGAAGC                                                >  SRR4302545.5776700/1‑94 (MQ=60)

                                                TACTGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCCATATTCGGCGATTCTTGCGCGCAtgccggctaaacatgtagct                                               >  SRR4302545.9697948/1‑74 (MQ=60)

                                                 ACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTG                                              <  SRR4302545.4185406/94‑1 (MQ=60)

                                                  CCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGCAGCTGA                                             >  SRR4302545.8836908/1‑94 (MQ=60)

                                                  CCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGA                                             >  SRR4302545.5298754/1‑94 (MQ=60)

                                                  CCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGA                                             <  SRR4302545.3538451/94‑1 (MQ=60)

                                                  CCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGA                                             <  SRR4302545.3538452/94‑1 (MQ=60)

                                                    GGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGA                                           >  SRR4302545.8544715/1‑94 (MQ=60)

                                                    GGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCGATACTCGGCGATTCTTGCGCGCCCTCTGGGAAAACAGGAAGCTGTGC                                           >  SRR4302545.5263363/1‑94 (MQ=60)

                                                      TCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAA                                         <  SRR4302545.3046521/94‑1 (MQ=60)

                                                       CTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAAC                                        <  SRR4302545.9515964/94‑1 (MQ=60)

                                                       CTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAAC                                        <  SRR4302545.2157369/94‑1 (MQ=60)

                                                        TTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAgaatcgccgagtatagcgg                                       <  SRR4302545.3034832/94‑20 (MQ=60)

                                                        TTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAAAAGGAAGCCGAGCAAAT                                       >  SRR4302545.6120671/1‑94 (MQ=60)

                                                           GCGCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGgcactggg                                    >  SRR4302545.4630428/1‑86 (MQ=60)

                                                             GCAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGC                                  <  SRR4302545.7090234/94‑1 (MQ=60)

                                                              CAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGGGATTCTTGCGCGCACGCTGGGAAAACAGAAAGCTGAGAAACTGGCGCG                                 >  SRR4302545.3885113/1‑94 (MQ=60)

                                                              CAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCG                                 <  SRR4302545.6652565/94‑1 (MQ=60)

                                                              CAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCG                                 <  SRR4302545.709160/94‑1 (MQ=60)

                                                              CAACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCG                                 >  SRR4302545.4782839/1‑94 (MQ=60)

                                                               AACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTTTTGCGCGCCAGCTTGCAAAACAGGAAACTGAGCAACTGGCGCGA                                >  SRR4302545.9098074/1‑94 (MQ=60)

                                                               AACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGA                                >  SRR4302545.2660151/1‑94 (MQ=60)

                                                               AACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGA                                >  SRR4302545.7577007/1‑94 (MQ=60)

                                                               AACACTATTAGCGCAATTTACCGGCGGGAAGCCGCCATACTCGGCGATTCTTGCGCGCACGCTGGCAACACAGGAAGATGAGCCACTGGgggcg                                >  SRR4302545.2293337/1‑89 (MQ=60)

                                                                ACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAA                               >  SRR4302545.10931833/1‑94 (MQ=60)

                                                                ACACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAA                               >  SRR4302545.4791213/1‑94 (MQ=60)

                                                                 CACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAG                              <  SRR4302545.860029/94‑1 (MQ=60)

                                                                 CACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAG                              >  SRR4302545.2880560/1‑94 (MQ=60)

                                                                 CACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACCGGCGCGAAG                              >  SRR4302545.2170911/1‑94 (MQ=60)

                                                                 CACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCCGGCAAAACAGGAAACTGAGCAACTCGCGCGAAG                              >  SRR4302545.1298973/1‑94 (MQ=60)

                                                                 CACTATTACCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAG                              <  SRR4302545.1995752/94‑1 (MQ=60)

                                                                  ACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACCGGCGCGAAGC                             >  SRR4302545.4508668/1‑94 (MQ=60)

                                                                  ACTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACCCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGC                             >  SRR4302545.4941155/1‑94 (MQ=60)

                                                                   CTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCA                            >  SRR4302545.9758081/1‑94 (MQ=60)

                                                                   CTATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCA                            <  SRR4302545.347432/94‑1 (MQ=60)

                                                                    TATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCCCGCTGGCCAAACAGGAAGCTGAGCAACTGGCGCGAAGCAA                           >  SRR4302545.6960011/1‑94 (MQ=60)

                                                                    TATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCCACTGGCGCGAAGAAA                           >  SRR4302545.6960012/1‑94 (MQ=60)

                                                                    TATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAA                           <  SRR4302545.4751143/94‑1 (MQ=60)

                                                                    TATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAA                           >  SRR4302545.5953336/1‑94 (MQ=60)

                                                                     ATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTTCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAG                          >  SRR4302545.8144272/1‑94 (MQ=60)

                                                                     ATTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAG                          >  SRR4302545.8376727/1‑94 (MQ=60)

                                                                      TTAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGG                         >  SRR4302545.1048368/1‑94 (MQ=60)

                                                                      TAAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGG                         <  SRR4302545.444748/94‑1 (MQ=60)

                                                                       TAGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGCTTCCTGCGCGCACGCCGGGAAAACACGAAGCTGAGCAACTGGCGCGAAGCAAAGC                        >  SRR4302545.9845191/1‑94 (MQ=60)

                                                                        AGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCCAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCC                       >  SRR4302545.7934465/1‑94 (MQ=60)

                                                                        AGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCC                       <  SRR4302545.7755931/94‑1 (MQ=60)

                                                                        AGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCC                       <  SRR4302545.6534020/94‑1 (MQ=60)

                                                                        AGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCC                       <  SRR4302545.4244321/94‑1 (MQ=60)

                                                                        AGCGCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCC                       <  SRR4302545.5705541/94‑1 (MQ=60)

                                                                         GCGCAATTTACCGGCGGGAAGCCGCTATCCTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCG                      <  SRR4302545.5723490/94‑1 (MQ=60)

                                                                           GCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCA                    >  SRR4302545.481371/1‑94 (MQ=60)

                                                                           GCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCA                    <  SRR4302545.9081077/94‑1 (MQ=60)

                                                                           GCAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGACGAACTGGCGCGAAGCAAGGCCccc                    >  SRR4302545.9109947/1‑91 (MQ=60)

                                                                            CAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTTTTGCGCGCACGCTGGCAAAAAAGGGAGCTGAGGAACTGGCGaggagccaggccggca                   >  SRR4302545.2162291/1‑78 (MQ=60)

                                                                            CAATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCAT                   >  SRR4302545.7081696/1‑94 (MQ=60)

                                                                             AATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATC                  <  SRR4302545.7006271/94‑1 (MQ=60)

                                                                              ATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCA                 <  SRR4302545.1492126/94‑1 (MQ=60)

                                                                              ATTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCA                 <  SRR4302545.1770994/94‑1 (MQ=60)

                                                                               TTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAG                <  SRR4302545.3851876/94‑1 (MQ=60)

                                                                               TTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAG                >  SRR4302545.4185804/1‑94 (MQ=60)

                                                                               TTTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAG                <  SRR4302545.6295141/94‑1 (MQ=60)

                                                                                TTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGC               <  SRR4302545.8043442/94‑1 (MQ=60)

                                                                                TTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGC               <  SRR4302545.6341659/94‑1 (MQ=60)

                                                                                TTACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCCCATCAGC               >  SRR4302545.7042005/1‑94 (MQ=60)

                                                                                 TACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCC              <  SRR4302545.1396526/94‑1 (MQ=60)

                                                                                 TACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCC              <  SRR4302545.5132240/94‑1 (MQ=60)

                                                                                  ACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGCAGCAAGGCCGCATCGGCCA             >  SRR4302545.3083025/1‑94 (MQ=60)

                                                                                  ACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCC             >  SRR4302545.3083024/1‑94 (MQ=60)

                                                                                  ACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCA             <  SRR4302545.8783222/94‑1 (MQ=60)

                                                                                  ACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCA             >  SRR4302545.9430221/1‑94 (MQ=60)

                                                                                  ACCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCA             <  SRR4302545.9351475/94‑1 (MQ=60)

                                                                                   CCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAGAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCAG            >  SRR4302545.548040/1‑94 (MQ=60)

                                                                                   CCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCAG            <  SRR4302545.704711/94‑1 (MQ=60)

                                                                                   CCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCAG            <  SRR4302545.704710/94‑1 (MQ=60)

                                                                                   CCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCAG            <  SRR4302545.1140461/94‑1 (MQ=60)

                                                                                   CCGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCAG            <  SRR4302545.3629725/94‑1 (MQ=60)

                                                                                    CGGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCAGC           >  SRR4302545.3084478/1‑94 (MQ=60)

                                                                                     GGCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCAGCG          <  SRR4302545.2818559/94‑1 (MQ=60)

                                                                                      GCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCAGCGA         >  SRR4302545.4332160/1‑94 (MQ=60)

                                                                                      GCGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCAGCGA         >  SRR4302545.2615517/1‑94 (MQ=60)

                                                                                      GCGGGAAACCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATaaggcagcgg         >  SRR4302545.5693609/1‑84 (MQ=60)

                                                                                       TGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAagatcagagatcggaagagcgtcgtgtagggaaagagtgtag        >  SRR4302545.3228634/1‑52 (MQ=60)

                                                                                       CGGGAAGCCGCTGTACTCGGCGATTCTTGCGCGCACGCTGGCAGAACAGGAAGCTGAGCAACTGGCGCGAAGCAAAACCGCATCAGCCAGCGAG        <  SRR4302545.9652530/94‑1 (MQ=60)

                                                                                       CGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGCGCAACTGGCGCGAAGCAAGGCCGCATCAGCCAGCGAG        >  SRR4302545.658594/1‑94 (MQ=60)

                                                                                       CGGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCAGCGAG        >  SRR4302545.1376686/1‑94 (MQ=60)

                                                                                        GGGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCAGCGAGA       <  SRR4302545.5225706/94‑1 (MQ=60)

                                                                                         GGAAGCCGCTATACTCGGCGATTCTTGCGCGCATGCTGGCAAAACAGGAAGCTGAGCAACAGGCGCGAAGCAAGGCCGCATCAGCCAGCGAGAA      <  SRR4302545.6212632/94‑1 (MQ=60)

                                                                                         GGAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCAGCGAGAA      <  SRR4302545.2809730/94‑1 (MQ=60)

                                                                                          GAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCAGCGAGAAT     <  SRR4302545.2667729/94‑1 (MQ=60)

                                                                                          GAAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCAGCGAGAAT     <  SRR4302545.2829364/94‑1 (MQ=60)

                                                                                          GAAACCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCAGCGAGAAT     <  SRR4302545.10737109/94‑1 (MQ=60)

                                                                                           AAGCCGCTATACTCGGCGATTCTTGCGCGCGCGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCAGCGAGAATA    <  SRR4302545.1305291/94‑1 (MQ=60)

                                                                                           AAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCAGCGAGAATA    <  SRR4302545.1304780/94‑1 (MQ=60)

                                                                                           AAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCAGCGAGAATA    >  SRR4302545.8390525/1‑94 (MQ=60)

                                                                                           AAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCAGCGAGAATA    >  SRR4302545.3715788/1‑94 (MQ=60)

                                                                                           AAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCAGCGAGAATA    >  SRR4302545.8847048/1‑94 (MQ=60)

                                                                                           AAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCAGCGAGAATA    <  SRR4302545.6458420/94‑1 (MQ=60)

                                                                                           AAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCAGCGAGAATA    >  SRR4302545.880170/1‑94 (MQ=60)

                                                                                           AAGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCAGCGAGAATA    >  SRR4302545.8566947/1‑94 (MQ=60)

                                                                                            AGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCAGCGAGAATAC   <  SRR4302545.3034207/94‑1 (MQ=60)

                                                                                            AGCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCAGCGAGAATAC   <  SRR4302545.6118115/94‑1 (MQ=60)

                                                                                             GCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCAGCGAGAATACT  <  SRR4302545.4198550/94‑1 (MQ=60)

                                                                                             GCCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCAGCGAGAATACT  <  SRR4302545.10141906/94‑1 (MQ=60)

GGTTCTGGAGATGACCGATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAACCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCAGCGAGAATACT  >  NC_000913/176339‑176525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`