Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 625,013 (C)5→4 coding (906/1176 nt) entC → isochorismate hydroxymutase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913625,0090C.97.3% 1288.9 / 17.9 299coding (902/1176 nt)entCisochorismate hydroxymutase 2
Reads supporting (aligned to +/- strand):  ref base C (6/2);  new base . (161/130);  total (167/132)
Fisher's exact test for biased strand distribution p-value = 4.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 344 total aligned reads displayed.

CCACGCCGACGCTGTGGCATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGACCCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTG  >  NC_000913/624916‑625098
                                                                                             |                                                                                         
 cacGCCGACGCTGTGGCATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCCGGCCTGTCTGCTGCATCCGAcc                                                                                          >  2:9406534/1‑94 (MQ=255)
  acGCCGACGCTGTGGCATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGAccc                                                                                         <  1:809586/94‑1 (MQ=255)
    gCCGACGCTGTGGCATCTCGCAACTCCCTTTGAAGGTACAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCCAcccgc                                                                                       >  2:711695/1‑92 (MQ=255)
     ccGACGCTGTGGCATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGAccccgc                                                                                      <  1:3376206/94‑3 (MQ=255)
     ccGACGCTGTGGCATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGAccccgc                                                                                      >  1:6175478/1‑92 (MQ=255)
      cGACGCTGTGGCATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGAccccgcg                                                                                     <  2:1315378/94‑4 (MQ=255)
       gACGCTGTGGCATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGAccccgcgc                                                                                    >  1:8438835/1‑90 (MQ=255)
       gACGCTGTGGCATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGACCCCcgcg                                                                                    >  2:9018330/1‑94 (MQ=255)
       gACGCTGTGGCATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCAGTCTGCTGCATCCGAccccgcgc                                                                                    >  1:5805704/1‑90 (MQ=255)
        gcGCTGTGGCATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCt                                                                                  <  1:8708497/93‑1 (MQ=255)
          gCTGTGGCATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGa                                                                                <  1:5096306/94‑1 (MQ=255)
          gCTGTGGCATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGa                                                                                <  2:10429954/94‑1 (MQ=255)
          gCTGTGGCATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCAGCTGCATCCGA‑CCCCGCGCTGa                                                                                <  1:6762503/94‑1 (MQ=255)
            tgtgGCATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGc                                                                              >  2:530882/1‑94 (MQ=255)
             gtgGCATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCg                                                                             >  1:3905667/1‑94 (MQ=255)
             gtgGCATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCg                                                                             <  1:776993/94‑1 (MQ=255)
              tgGCATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCgg                                                                            >  2:1597443/1‑94 (MQ=255)
               ggCATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGt                                                                           >  1:3445224/1‑94 (MQ=255)
                 cATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGttt                                                                         <  2:1119029/94‑1 (MQ=255)
                  aTCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTc                                                                        >  2:2796982/1‑94 (MQ=255)
                  aTCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTc                                                                        >  1:4908738/1‑94 (MQ=255)
                  aTCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTc                                                                        >  1:10024475/1‑94 (MQ=255)
                  aTCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCG‑CCCCCGCGCTGAGCGGTTTc                                                                        >  2:7440667/1‑94 (MQ=255)
                   tctcGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTcc                                                                       <  2:4737543/94‑1 (MQ=255)
                    ctcGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTccc                                                                      <  1:2228226/94‑1 (MQ=255)
                      cGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGc                                                                    >  1:5036572/1‑94 (MQ=255)
                      cGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGGTTCCCGc                                                                    >  1:982155/1‑94 (MQ=255)
                         aaCTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATc                                                                 >  1:4311525/1‑94 (MQ=255)
                           cTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAg                                                               <  1:4070648/94‑1 (MQ=255)
                              ccTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGcc                                                            >  2:4200635/1‑94 (MQ=255)
                              ccTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGcc                                                            >  1:10326431/1‑94 (MQ=255)
                              ccTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGcc                                                            <  1:9443295/94‑1 (MQ=255)
                                 ttGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCcgcg                                                         <  1:7146787/94‑1 (MQ=255)
                                   gAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGAc                                                       >  1:1300792/1‑94 (MQ=255)
                                     aGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGAccc                                                     >  1:3294654/1‑94 (MQ=255)
                                     aGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGAccc                                                     >  2:5821595/1‑94 (MQ=255)
                                     aGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGAccc                                                     <  2:66301/94‑1 (MQ=255)
                                      ggTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCa                                                    <  1:2759634/94‑1 (MQ=255)
                                        tAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAgg                                                  <  2:2249271/94‑1 (MQ=255)
                                         aaaGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGCCCCAGGt                                                 >  1:1446610/1‑94 (MQ=255)
                                         aaaGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGt                                                 >  1:1667872/1‑94 (MQ=255)
                                          aaGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCCGTTTCCCGCATCAGGCCGCGACCCCGGtt                                                >  2:2871925/1‑94 (MQ=255)
                                           aGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTa                                               <  1:3608844/94‑1 (MQ=255)
                                            gCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTAt                                              <  1:7737309/94‑1 (MQ=255)
                                             cGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTAtt                                             >  1:5347667/1‑94 (MQ=255)
                                              gAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTg                                            <  2:828722/94‑1 (MQ=255)
                                              gAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTg                                            >  1:3921127/1‑94 (MQ=255)
                                              gAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTg                                            >  2:7777885/1‑94 (MQ=255)
                                                aTTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCt                                          >  1:2328606/1‑94 (MQ=255)
                                                   cGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCCCGACCCAGGTTATTGCTGaa                                       >  1:1527093/1‑94 (MQ=255)
                                                    gCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAAc                                      >  2:3208018/1‑94 (MQ=255)
                                                    gCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAAc                                      >  1:10460399/1‑94 (MQ=255)
                                                    gCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAAc                                      >  2:2598245/1‑94 (MQ=255)
                                                     cAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACt                                     >  2:4864039/1‑94 (MQ=255)
                                                     cAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACt                                     >  1:2863964/1‑94 (MQ=255)
                                                     cAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACt                                     <  1:9564772/94‑1 (MQ=255)
                                                      aaGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTg                                    >  2:6850236/1‑94 (MQ=255)
                                                       aGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTgg                                   >  2:8166881/1‑94 (MQ=255)
                                                       aGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTga                                   >  2:8063116/1‑93 (MQ=255)
                                                        gAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGa                                  <  1:2462547/94‑1 (MQ=255)
                                                        gAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGa                                  >  1:1985195/1‑94 (MQ=255)
                                                         aaaaCGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGaa                                 <  2:2667288/94‑1 (MQ=255)
                                                         aaaaCGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGaa                                 <  2:6401369/94‑1 (MQ=255)
                                                         aaaaCGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGaa                                 <  2:10070931/94‑1 (MQ=255)
                                                          aaaCGCACTGACTCTGGCCTGTCTGCTGCATCCGACCCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGaa                                 >  2:5065011/1‑94 (MQ=255)
                                                           aaCGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAAcc                               <  1:179767/94‑1 (MQ=255)
                                                           aaCGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAAcc                               <  2:1355038/94‑1 (MQ=255)
                                                           aaCGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAAcc                               >  2:3558787/1‑94 (MQ=255)
                                                             cGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGt                             >  2:7642960/1‑94 (MQ=255)
                                                              gCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGtt                            >  2:4353939/1‑94 (MQ=255)
                                                              gCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGtt                            <  2:1087362/94‑1 (MQ=255)
                                                               cACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTc                           <  1:7957397/94‑1 (MQ=255)
                                                               cACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTc                           >  2:6563397/1‑94 (MQ=255)
                                                                aCTGACTCTGGCCTGTCTGCTGCATCCGACCCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTc                           <  1:7422699/94‑1 (MQ=255)
                                                                 cTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGa                         <  1:70308/94‑1 (MQ=255)
                                                                   gACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGAcc                       >  2:9616326/1‑94 (MQ=255)
                                                                    gctctGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACcg                      <  1:5394420/93‑1 (MQ=255)
                                                                    aCTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACcg                      <  1:9035339/94‑1 (MQ=255)
                                                                     ctctGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACcgc                     >  2:5953472/1‑94 (MQ=255)
                                                                     ctctGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACcgc                     >  1:693442/1‑94 (MQ=255)
                                                                      tctGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACcgcg                    >  2:1476361/1‑94 (MQ=255)
                                                                        tGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGaa                  >  1:10161571/1‑94 (MQ=255)
                                                                         ggCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAAc                 >  2:9810093/1‑94 (MQ=255)
                                                                         ggCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAAc                 <  1:5997075/94‑1 (MQ=255)
                                                                           gcTGGCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTg               <  2:3449337/93‑1 (MQ=255)
                                                                            cTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGt              >  1:825995/1‑94 (MQ=255)
                                                                            cTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGt              >  1:10087533/1‑94 (MQ=255)
                                                                            cTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGt              >  2:4641125/1‑94 (MQ=255)
                                                                             tGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGtt             >  1:8899237/1‑94 (MQ=255)
                                                                              ttCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGttt            <  2:2117161/93‑1 (MQ=255)
                                                                              gTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGttt            <  2:9149978/94‑1 (MQ=255)
                                                                               tCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTg           <  2:1828482/94‑1 (MQ=255)
                                                                                ctgctgCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGACCCCTTCTCCCCCGAACTGTTTgg          >  1:7842737/1‑94 (MQ=255)
                                                                                ctgctgCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTgg          <  1:6554771/94‑1 (MQ=255)
                                                                                   ctgcATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTggcgg       <  2:3962763/94‑1 (MQ=255)
                                                                                      cATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCAt    <  2:1744769/94‑1 (MQ=255)
                                                                                      cATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCAt    <  2:8419890/94‑1 (MQ=255)
                                                                                      cATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCAt    >  1:5702530/1‑94 (MQ=255)
                                                                                       aTCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCAtt   >  2:6812078/1‑94 (MQ=255)
                                                                                         tccgcccccGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATtg  <  1:373541/90‑1 (MQ=255)
                                                                                             |                                                                                         
CCACGCCGACGCTGTGGCATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGACCCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTG  >  NC_000913/624916‑625098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 203 total aligned reads displayed.
GCCGACGCTGTGGCATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGACCCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTGTGGGTTGG  >  NC_000913/624920‑625106
                                                                                             |                                                                                             
GCCGACGCTGTGGCATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGACCCCG                                                                                               <  SRR4302545.10894755/94‑1 (MQ=60)
GCCGACGCTGTGGCATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGACCCCG                                                                                               <  SRR4302545.7102432/94‑1 (MQ=60)
  CGACGCTGTGGCATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCG                                                                                            >  SRR4302545.10035251/1‑94 (MQ=60)
  CGACGCTGTGGCATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCG                                                                                            <  SRR4302545.1983669/94‑1 (MQ=60)
  CGACGCTGTGGCATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCG                                                                                            >  SRR4302545.4177449/1‑94 (MQ=60)
   GACGCTGTGGCATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGC                                                                                           >  SRR4302545.8682820/1‑94 (MQ=60)
   GACGCTGTGGCATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCAGTCTGCTGCATCCGA‑CCCCGCGC                                                                                           >  SRR4302545.5961886/1‑94 (MQ=60)
    GCGCTGTGGCATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCT                                                                                          <  SRR4302545.8961138/94‑1 (MQ=60)
    ACGCTGTGGCATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCT                                                                                          <  SRR4302545.3750296/94‑1 (MQ=60)
    ACGCTGTGGCATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCT                                                                                          <  SRR4302545.3463379/94‑1 (MQ=60)
     CGCTGTGGCATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTG                                                                                         >  SRR4302545.3644434/1‑94 (MQ=60)
     CGCTGTGGCATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTG                                                                                         >  SRR4302545.155360/1‑94 (MQ=60)
      GCTGTGGCATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGA                                                                                        <  SRR4302545.5226381/94‑1 (MQ=60)
      GCTGTGGCATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGA                                                                                        >  SRR4302545.2165250/1‑94 (MQ=60)
      GCTGTGGCATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGA                                                                                        <  SRR4302545.5226382/94‑1 (MQ=60)
      GCTGTGGCATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCAGCTGCATCCGA‑CCCCGCGCTGA                                                                                        <  SRR4302545.6947934/94‑1 (MQ=60)
       CTGTGGCATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAG                                                                                       >  SRR4302545.5904652/1‑94 (MQ=60)
       CTGTGGCATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAG                                                                                       <  SRR4302545.2407000/94‑1 (MQ=60)
       CTGTGGCATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAG                                                                                       >  SRR4302545.8105207/1‑94 (MQ=60)
       CTGTGGCATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAG                                                                                       <  SRR4302545.6931946/94‑1 (MQ=60)
         GTGGCATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCG                                                                                     >  SRR4302545.4003145/1‑94 (MQ=60)
         GTGGCATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCG                                                                                     <  SRR4302545.794470/94‑1 (MQ=60)
         GTGGAATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCG                                                                                     >  SRR4302545.7979149/1‑94 (MQ=60)
          TGGGATCTCGCAACTCCCTTTGAAGGCATTGCGAATTCGCAAGAAAACGCACTGACTCTTGCCTGTCTGCTGCATCCGACCCCCGCGCTGAGCG                                                                                     >  SRR4302545.7441166/1‑94 (MQ=60)
          TGGCATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGG                                                                                    >  SRR4302545.634146/1‑94 (MQ=60)
           GGCATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGT                                                                                   >  SRR4302545.3529531/1‑94 (MQ=60)
            GCATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGATGCATCCGA‑CCCCGCTCTGAGCGGTT                                                                                  >  SRR4302545.3613149/1‑94 (MQ=60)
             CATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTT                                                                                 <  SRR4302545.7141904/94‑1 (MQ=60)
             CATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTT                                                                                 <  SRR4302545.403218/94‑1 (MQ=60)
              ATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTC                                                                                >  SRR4302545.5033318/1‑94 (MQ=60)
              ATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTC                                                                                >  SRR4302545.10321540/1‑94 (MQ=60)
              ATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTC                                                                                >  SRR4302545.10622133/1‑94 (MQ=60)
                CTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCC                                                                              >  SRR4302545.9744553/1‑94 (MQ=60)
                CTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCC                                                                              <  SRR4302545.2282594/94‑1 (MQ=60)
                 TCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTAGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCG                                                                             >  SRR4302545.4106489/1‑94 (MQ=60)
                  CGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGC                                                                            >  SRR4302545.10170765/1‑94 (MQ=60)
                  CGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGC                                                                            >  SRR4302545.2485169/1‑94 (MQ=60)
                  CGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGC                                                                            >  SRR4302545.5164370/1‑94 (MQ=60)
                  CGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGGTTCCCGC                                                                            >  SRR4302545.1004038/1‑94 (MQ=60)
                    CAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCAT                                                                          >  SRR4302545.7150392/1‑94 (MQ=60)
                     AACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATC                                                                         >  SRR4302545.2730177/1‑94 (MQ=60)
                     AACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATC                                                                         >  SRR4302545.1944527/1‑94 (MQ=60)
                     AACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATC                                                                         >  SRR4302545.1703091/1‑94 (MQ=60)
                     AACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATC                                                                         >  SRR4302545.4420113/1‑94 (MQ=60)
                     AACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATC                                                                         >  SRR4302545.7097632/1‑94 (MQ=60)
                       CTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAG                                                                       <  SRR4302545.7972721/94‑1 (MQ=60)
                       CTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAG                                                                       <  SRR4302545.4172570/94‑1 (MQ=60)
                        TCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGG                                                                      >  SRR4302545.9726423/1‑94 (MQ=60)
                          CCTTTGAAGGTAAAGCGCATTCGCAAGAAAACGCACTGTCTCTGGCCTGTCTGCGCCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGACC                                                                    <  SRR4302545.1713443/94‑1 (MQ=60)
                          CCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGGCTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCC                                                                    <  SRR4302545.1713442/94‑1 (MQ=60)
                          CCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCC                                                                    <  SRR4302545.9720828/94‑1 (MQ=60)
                          CCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCC                                                                    >  SRR4302545.10630910/1‑94 (MQ=60)
                          CCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCC                                                                    <  SRR4302545.9720827/94‑1 (MQ=60)
                            TTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGC                                                                  >  SRR4302545.7802537/1‑94 (MQ=60)
                            TTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGC                                                                  <  SRR4302545.9166774/94‑1 (MQ=60)
                             TTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCG                                                                 <  SRR4302545.7345132/94‑1 (MQ=60)
                             TTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCG                                                                 <  SRR4302545.7345134/94‑1 (MQ=60)
                             TTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCG                                                                 >  SRR4302545.4744922/1‑94 (MQ=60)
                             TTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCG                                                                 <  SRR4302545.7345131/94‑1 (MQ=60)
                             TTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCAGCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCG                                                                 <  SRR4302545.10439085/94‑1 (MQ=60)
                              TGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGA                                                                >  SRR4302545.1364979/1‑94 (MQ=60)
                              TGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGA                                                                >  SRR4302545.5233303/1‑94 (MQ=60)
                              TGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCCCATCAGGCCGCGA                                                                >  SRR4302545.9072438/1‑94 (MQ=60)
                              TGAACCTAAAGCGAATTCGCAAGACAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGCTTCCCGCATCAGGCCGGGA                                                                >  SRR4302545.5233302/1‑94 (MQ=60)
                               GAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGAC                                                               >  SRR4302545.1329679/1‑94 (MQ=60)
                               GAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGAC                                                               >  SRR4302545.914579/1‑94 (MQ=60)
                               GAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGAC                                                               >  SRR4302545.1673315/1‑94 (MQ=60)
                               GAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGAC                                                               >  SRR4302545.3466975/1‑94 (MQ=60)
                               GAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGAC                                                               >  SRR4302545.4574797/1‑94 (MQ=60)
                                 TGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCCGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGTGGTCTCGCATCAGGCCTCGACCC                                                             >  SRR4302545.6799171/1‑94 (MQ=60)
                                 AGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCC                                                             <  SRR4302545.5038272/94‑1 (MQ=60)
                                 AGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCC                                                             >  SRR4302545.3375309/1‑94 (MQ=60)
                                  GGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCA                                                            <  SRR4302545.2826222/94‑1 (MQ=60)
                                  GGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCA                                                            <  SRR4302545.7994912/94‑1 (MQ=60)
                                  GGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCA                                                            <  SRR4302545.1537056/94‑1 (MQ=60)
                                  GGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCA                                                            <  SRR4302545.1537055/94‑1 (MQ=60)
                                   GTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAG                                                           <  SRR4302545.8263471/94‑1 (MQ=60)
                                    TAAAGCGAATTCGCAAGAAAACGCACTGCCTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGG                                                          <  SRR4302545.3147394/94‑1 (MQ=60)
                                    TAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGG                                                          <  SRR4302545.5288452/94‑1 (MQ=60)
                                     AAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGACCCCCGCGCTGAGCGGTTTCCTGCATCAGGCCGCGACCCAGG                                                          >  SRR4302545.10698912/1‑94 (MQ=60)
                                     AAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGCCCCAGGT                                                         >  SRR4302545.1479628/1‑94 (MQ=60)
                                     AAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGT                                                         >  SRR4302545.1707314/1‑94 (MQ=60)
                                      AAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTT                                                        <  SRR4302545.925790/94‑1 (MQ=60)
                                       AGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTA                                                       <  SRR4302545.3696819/94‑1 (MQ=60)
                                       AGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTA                                                       <  SRR4302545.3843075/94‑1 (MQ=60)
                                        GCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTAT                                                      <  SRR4302545.7956297/94‑1 (MQ=60)
                                        GCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTAT                                                      <  SRR4302545.7179378/94‑1 (MQ=60)
                                         CGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATT                                                     >  SRR4302545.5486581/1‑94 (MQ=60)
                                         CGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATT                                                     >  SRR4302545.5486580/1‑94 (MQ=60)
                                          GAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTG                                                    >  SRR4302545.4018992/1‑94 (MQ=60)
                                          GAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTG                                                    >  SRR4302545.132012/1‑94 (MQ=60)
                                          GAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGATATTG                                                    >  SRR4302545.4018991/1‑94 (MQ=60)
                                           ctgatgcggGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGC                                                   >  SRR4302545.7342672/10‑94 (MQ=60)
                                            ATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGCCCCAGGTTATTGCT                                                  <  SRR4302545.5171961/94‑1 (MQ=60)
                                            ATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCT                                                  >  SRR4302545.2385295/1‑94 (MQ=60)
                                               CGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCCCGACCCAGGTTATTGCTGAA                                               >  SRR4302545.1562772/1‑94 (MQ=60)
                                                GCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAAC                                              <  SRR4302545.2331037/94‑1 (MQ=60)
                                                GCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAAC                                              >  SRR4302545.10768806/1‑94 (MQ=60)
                                                 CAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACT                                             >  SRR4302545.2933189/1‑94 (MQ=60)
                                                 CAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACT                                             >  SRR4302545.1772560/1‑94 (MQ=60)
                                                 CAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACT                                             <  SRR4302545.793688/94‑1 (MQ=60)
                                                 CAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGGTTCCCGCATCAGGCGGCGACCCAGGTTATTGCTGAACT                                             >  SRR4302545.7715532/1‑94 (MQ=60)
                                                  aaaaacacgcactgaaaataGCCTGTATGCTGCATCAGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCaaggatatagatgttcag                                            <  SRR4302545.9685145/74‑19 (MQ=51)
                                                  AAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTG                                            >  SRR4302545.1965447/1‑94 (MQ=60)
                                                  AAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTG                                            <  SRR4302545.903588/94‑1 (MQ=60)
                                                  AAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTG                                            >  SRR4302545.2340616/1‑94 (MQ=60)
                                                  AAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTG                                            <  SRR4302545.10184957/94‑1 (MQ=60)
                                                    GAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGA                                          <  SRR4302545.2522168/94‑1 (MQ=60)
                                                    GAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGA                                          <  SRR4302545.5762096/94‑1 (MQ=60)
                                                    GAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGA                                          >  SRR4302545.2034337/1‑94 (MQ=60)
                                                    GAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGA                                          <  SRR4302545.2872841/94‑1 (MQ=60)
                                                     AAAAGGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGGTATTGCTGAACTGGAA                                         >  SRR4302545.3196427/1‑94 (MQ=60)
                                                     AAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAA                                         >  SRR4302545.10061569/1‑94 (MQ=60)
                                                     AAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAA                                         >  SRR4302545.3196426/1‑94 (MQ=60)
                                                      AAACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAAC                                        <  SRR4302545.3675526/94‑1 (MQ=60)
                                                       AACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACC                                       <  SRR4302545.183601/94‑1 (MQ=60)
                                                       AACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACC                                       <  SRR4302545.3411922/94‑1 (MQ=60)
                                                       AACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACC                                       <  SRR4302545.5496325/94‑1 (MQ=60)
                                                       AACGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCAGCATCAGGCCGCGACCCAGGTTATTGCTTAACTGGAACC                                       <  SRR4302545.5681052/94‑1 (MQ=60)
                                                       AACGCACTGACTATGGCCTGTCTGCTGCATCCGA‑CACCGCGCTGAGCGGTTTCCCGCATCATACAGCAAACGACGTTATTGCTGAACTGGAACC                                       >  SRR4302545.8393855/1‑94 (MQ=41)
                                                         CGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGT                                     <  SRR4302545.8632717/94‑1 (MQ=60)
                                                         CGCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGGTATTGCTGAACTGGAACCGT                                     >  SRR4302545.2208588/1‑94 (MQ=60)
                                                          GCACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCATGTTATTGCTGAACTGGAACCGTT                                    >  SRR4302545.6006555/1‑94 (MQ=60)
                                                          GCACTGACTCTGGCCTGTCTGATGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTT                                    >  SRR4302545.10491500/1‑94 (MQ=60)
                                                           CACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTC                                   <  SRR4302545.8183557/94‑1 (MQ=60)
                                                           CACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTC                                   <  SRR4302545.8394623/94‑1 (MQ=60)
                                                           CACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTC                                   <  SRR4302545.6171632/94‑1 (MQ=60)
                                                            ACTGACTCTGGCCTGTCTGCTGCATCCGACCCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTC                                   <  SRR4302545.7632556/94‑1 (MQ=60)
                                                            ACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCG                                  >  SRR4302545.3795800/1‑94 (MQ=60)
                                                            ACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCG                                  >  SRR4302545.7616372/1‑94 (MQ=60)
                                                            ACTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCG                                  >  SRR4302545.61259/1‑94 (MQ=60)
                                                             CTGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGA                                 <  SRR4302545.71880/94‑1 (MQ=60)
                                                              tccCTCTGGCCCGTCTGCTGCATCGGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCGGAACTGGAACCGTTCGAC                                <  SRR4302545.3150534/91‑1 (MQ=60)
                                                              TGTCTCTGGCCTGTCTGCTGCATCCGACCCACGCGCTGCGCTGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTAGA                                 <  SRR4302545.4555130/94‑1 (MQ=60)
                                                              TGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACTGTTCGAC                                >  SRR4302545.4754505/1‑94 (MQ=60)
                                                              TGACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGAC                                >  SRR4302545.10475989/1‑94 (MQ=60)
                                                               GACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACC                               <  SRR4302545.3330251/94‑1 (MQ=60)
                                                               GACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACC                               <  SRR4302545.755550/94‑1 (MQ=60)
                                                               GACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACC                               >  SRR4302545.5729091/1‑94 (MQ=60)
                                                                GCTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCG                              <  SRR4302545.5534961/94‑1 (MQ=60)
                                                                ACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCG                              >  SRR4302545.85295/1‑94 (MQ=60)
                                                                ACTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCG                              <  SRR4302545.9298682/94‑1 (MQ=60)
                                                                 CTCTGGCCTGTCTGCTGCATCCGACCCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCT                              >  SRR4302545.3932998/1‑94 (MQ=60)
                                                                 CTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGC                             >  SRR4302545.5333768/1‑94 (MQ=60)
                                                                 CTCTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGC                             >  SRR4302545.708976/1‑94 (MQ=60)
                                                                   CTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGA                           <  SRR4302545.2859009/94‑1 (MQ=60)
                                                                   CTGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGA                           >  SRR4302545.8407048/1‑94 (MQ=60)
                                                                    TGGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAA                          >  SRR4302545.10462030/1‑94 (MQ=60)
                                                                     GGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAAC                         <  SRR4302545.6158923/94‑1 (MQ=60)
                                                                     GGCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAAC                         >  SRR4302545.341366/1‑94 (MQ=60)
                                                                      GCCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACT                        <  SRR4302545.2292055/94‑1 (MQ=60)
                                                                       CCTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTG                       <  SRR4302545.5908717/94‑1 (MQ=60)
                                                                        CTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGT                      >  SRR4302545.10385873/1‑94 (MQ=60)
                                                                        CTGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGT                      >  SRR4302545.844525/1‑94 (MQ=60)
                                                                         TGTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTT                     >  SRR4302545.9157904/1‑94 (MQ=60)
                                                                          GTCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTT                    <  SRR4302545.8959707/94‑1 (MQ=60)
                                                                           TCTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTG                   >  SRR4302545.2565233/1‑94 (MQ=60)
                                                                            CTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGACCCCTTCTCCCCCGAACTGTTTGG                  >  SRR4302545.8065098/1‑94 (MQ=60)
                                                                            CTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGG                  >  SRR4302545.8065097/1‑94 (MQ=60)
                                                                            CTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGG                  <  SRR4302545.6734090/94‑1 (MQ=60)
                                                                            CTGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGG                  >  SRR4302545.6123929/1‑94 (MQ=60)
                                                                             ctgcggcaggcgaCCCCGCGCGGATCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGG                  <  SRR4302545.6734091/81‑1 (MQ=60)
                                                                             TGCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGC                 >  SRR4302545.5044687/1‑94 (MQ=60)
                                                                              GCTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCG                <  SRR4302545.4030582/94‑1 (MQ=60)
                                                                               tctgcacccgaCCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCG                <  SRR4302545.10326227/83‑1 (MQ=60)
                                                                               CTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGG               <  SRR4302545.10738555/94‑1 (MQ=60)
                                                                               CTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGG               >  SRR4302545.3874595/1‑94 (MQ=60)
                                                                               CTGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGG               >  SRR4302545.10852868/1‑94 (MQ=60)
                                                                                TGCATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGC              >  SRR4302545.8254838/1‑94 (MQ=60)
                                                                                  CATCCGACCCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCA             >  SRR4302545.10398934/1‑94 (MQ=60)
                                                                                  CATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCAT            >  SRR4302545.5855265/1‑94 (MQ=60)
                                                                                  CATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCAT            >  SRR4302545.10336451/1‑94 (MQ=60)
                                                                                  CATCCGA‑CCCCGCGCTGAGCGGGTTCCCGCATCAGGCCGCGACCCCGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCCT            >  SRR4302545.5345491/1‑94 (MQ=60)
                                                                                   ATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATT           >  SRR4302545.8646257/1‑94 (MQ=60)
                                                                                   ATCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATT           >  SRR4302545.9652293/1‑94 (MQ=60)
                                                                                    TCCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTG          >  SRR4302545.8111544/1‑94 (MQ=60)
                                                                                    TCCG‑CCCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTG          <  SRR4302545.381755/94‑1 (MQ=60)
                                                                                    TCCG‑CCCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTG          <  SRR4302545.2786833/94‑1 (MQ=60)
                                                                                     CCGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTGT         <  SRR4302545.6327543/94‑1 (MQ=60)
                                                                                      CGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTGTG        >  SRR4302545.28806/1‑94 (MQ=60)
                                                                                      CGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTGTG        >  SRR4302545.270222/1‑94 (MQ=60)
                                                                                      CGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTGTG        <  SRR4302545.1989420/94‑1 (MQ=60)
                                                                                      CGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTGTG        >  SRR4302545.7220203/1‑94 (MQ=60)
                                                                                      CGA‑CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTGTG        >  SRR4302545.1441480/1‑94 (MQ=60)
                                                                                      CGA‑CCCCGCGCTGAGCGGTTTCCCGCATATGGCCGCGACCCAGGCTATTGCTGAACTGGAACCGTTCGACCGCGAACTTTTTGGCcggattgcg        >  SRR4302545.9254420/1‑85 (MQ=60)
                                                                                        gaCCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTGTGG       <  SRR4302545.1053342/92‑1 (MQ=60)
                                                                                        gaCCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTGTGG       >  SRR4302545.8851633/3‑94 (MQ=60)
                                                                                        gaCCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTGTGG       <  SRR4302545.7521953/92‑1 (MQ=60)
                                                                                        gaCCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTGTGG       >  SRR4302545.10719798/3‑94 (MQ=60)
                                                                                        gaCCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTGTGG       >  SRR4302545.1909402/3‑94 (MQ=60)
                                                                                        gaCCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTGTGG       >  SRR4302545.8571332/3‑94 (MQ=60)
                                                                                        gaCCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTGTGG       <  SRR4302545.10592849/92‑1 (MQ=60)
                                                                                         ACCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTGTGGG      <  SRR4302545.1244026/94‑1 (MQ=60)
                                                                                         ACCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTGTGGG      <  SRR4302545.9156581/94‑1 (MQ=60)
                                                                                          CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTGTGGGT     >  SRR4302545.3318485/1‑94 (MQ=60)
                                                                                          CCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGGTCGACCGCGAACTGTTTGGCGGCATTGTGGGT     >  SRR4302545.9262173/1‑94 (MQ=60)
                                                                                           CCCGCGCTGAGCGGTTTCCCGCATCAGTCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTGTGGGTT    <  SRR4302545.10846356/94‑1 (MQ=60)
                                                                                           CCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTGTGGGTT    >  SRR4302545.813683/1‑94 (MQ=60)
                                                                                            CCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTGTGGGTTG   >  SRR4302545.4962748/1‑94 (MQ=60)
                                                                                             CGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTGTGGGTTGG  >  SRR4302545.5401016/1‑94 (MQ=60)
                                                                                             |                                                                                             
GCCGACGCTGTGGCATCTCGCAACTCCCTTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGACCCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTGTGGGTTGG  >  NC_000913/624920‑625106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑