Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 967,279 T→C V479A (GTG→GCG)  msbA → ATP‑binding transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913967,2790TC99.6% 830.0 / ‑5.2 245V479A (GTG→GCG) msbAATP‑binding transport protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (111/133);  total (112/133)
Fisher's exact test for biased strand distribution p-value = 4.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01

Only 100 of 267 total aligned reads displayed.

CAAATTGAAGAAGCGGCGCGTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGTGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCTGGACGAAGCTACCTC  >  NC_000913/967188‑967369
                                                                                           |                                                                                          
cAAATTGAAGAAGCGGCGCGTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGgcgcgc                                                                                          <  2:2348109/94‑1 (MQ=255)
 aaaTTGAAGAAGCGGCGCGTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGcgct                                                                                         >  1:10579346/1‑94 (MQ=255)
 aaaTTGAAGAAGCGGCGCGTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGcgct                                                                                         <  1:3478457/94‑1 (MQ=255)
    ttGAAGAAGCGGCGCGTATGGCCTACGCCATGGACTTCATCAATAAGATGGATCACGGTCTCGATCCAGTGATTGGTGAAAACGGCGCgctgct                                                                                      >  2:5103061/1‑94 (MQ=255)
    ttGAAGAAGCGGCGCGTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCgctgct                                                                                      >  1:1736233/1‑94 (MQ=255)
        agaagCGGCGCGTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGctctct                                                                                  >  2:1466524/1‑94 (MQ=255)
         gaagCGGCGCGTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTg                                                                                 >  1:6646832/1‑94 (MQ=255)
          aagCGGCGCGTATGACCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAATCGGCGCGCTGCTCTCTgg                                                                                <  1:430440/94‑1 (MQ=255)
           agCGTCGCGTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGc                                                                               <  2:5023125/94‑1 (MQ=255)
           agCGGCGCGTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGc                                                                               >  2:10504460/1‑94 (MQ=255)
             cggcgCGTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCgg                                                                             <  1:114499/94‑1 (MQ=255)
             cggcgCGTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCgg                                                                             <  2:7574383/94‑1 (MQ=255)
             cggcgCGTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCgg                                                                             >  1:2597625/1‑94 (MQ=255)
             cggcgCGTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCgg                                                                             >  2:3027670/1‑94 (MQ=255)
                cgcgTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGgtca                                                                          >  2:70717/1‑94 (MQ=255)
                 gcgTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGgtcag                                                                         >  1:2767218/1‑94 (MQ=255)
                   gTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGgtcagcg                                                                       <  1:7156024/94‑1 (MQ=255)
                    tATGGCCTATGCCATGGTCTTCATCAATAAGATTGATAACGGTCTTGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGgtcagcgt                                                                      >  1:9665639/1‑94 (MQ=255)
                     aTGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGgtcagcgtc                                                                     >  2:8119001/1‑94 (MQ=255)
                       ggCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGgtcagcgtcag                                                                   <  2:5414938/94‑1 (MQ=255)
                         ccTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGtcagcgtcagcg                                                                 >  2:1791966/1‑94 (MQ=255)
                         ccTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGtcagcgtcagcg                                                                 <  2:6295698/94‑1 (MQ=255)
                          cTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTcagcgtcagcgt                                                                <  2:4424576/94‑1 (MQ=255)
                           tACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTa                                                               >  1:8509958/1‑94 (MQ=255)
                            aCGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTAt                                                              <  2:6107581/94‑1 (MQ=255)
                            aCGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTAt                                                              <  2:8620925/94‑1 (MQ=255)
                            aCGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTAt                                                              <  1:3824646/94‑1 (MQ=255)
                              gCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTg                                                            >  2:1952665/1‑94 (MQ=255)
                              gCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTg                                                            >  2:7735961/1‑94 (MQ=255)
                               tcATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGc                                                           <  1:3539410/93‑1 (MQ=255)
                                cATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCt                                                          >  2:9831937/1‑94 (MQ=255)
                                cATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCt                                                          <  1:8042732/94‑1 (MQ=255)
                                cATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCt                                                          >  2:1848031/1‑94 (MQ=255)
                                 aTGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTa                                                         >  1:7042426/1‑94 (MQ=255)
                                   ggACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATc                                                       <  1:10122235/94‑1 (MQ=255)
                                   ggACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATc                                                       <  1:4841366/94‑1 (MQ=255)
                                   ggACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATc                                                       <  2:6458367/94‑1 (MQ=255)
                                    gACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCg                                                      >  1:5022177/1‑94 (MQ=255)
                                      cTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCt                                                    >  1:3627691/1‑94 (MQ=255)
                                      cTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCt                                                    >  1:8202004/1‑94 (MQ=255)
                                      cTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCt                                                    >  2:6925625/1‑94 (MQ=255)
                                       ttCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTc                                                   <  2:699236/94‑1 (MQ=255)
                                        taatcaATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCg                                                  >  2:7342217/3‑94 (MQ=255)
                                         catcaATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGa                                                 >  2:8803773/1‑94 (MQ=255)
                                         catcaATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGa                                                 <  2:3685215/94‑1 (MQ=255)
                                          atcaATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAg                                                >  2:6021512/1‑94 (MQ=255)
                                          atcaATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAg                                                <  1:6118082/94‑1 (MQ=255)
                                          atcaATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCGCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAg                                                <  1:5617204/94‑1 (MQ=255)
                                           tcaATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGc                                               >  1:5279806/1‑94 (MQ=255)
                                             aaTAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCt                                             <  1:9510601/94‑1 (MQ=255)
                                              aTAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCtt                                            <  2:2984818/94‑1 (MQ=255)
                                              aTAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCtt                                            >  1:4353616/1‑94 (MQ=255)
                                                aaGATGTATAACGGTCTCGATACAGTGATTGGTTAAAACGGCGCGCTGCCCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCttgt                                          <  2:48327/94‑1 (MQ=39)
                                                aaGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCttgt                                          <  2:1312795/94‑1 (MQ=255)
                                                 aGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCttgtt                                         <  2:3923909/94‑1 (MQ=255)
                                                 aGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCttgtt                                         <  1:4774558/94‑1 (MQ=255)
                                                  gATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCttgttg                                        >  2:10266957/1‑94 (MQ=255)
                                                   aTGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGc                                       <  1:10179101/94‑1 (MQ=255)
                                                   aTGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGc                                       >  1:2880404/1‑94 (MQ=255)
                                                     ggATAGCGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGt                                     <  2:10024749/94‑1 (MQ=255)
                                                       aTAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGa                                   <  1:8643148/94‑1 (MQ=255)
                                                        tAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGAt                                  >  2:1671799/1‑94 (MQ=255)
                                                         aaCGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATa                                 <  2:269740/94‑1 (MQ=255)
                                                         aaCGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATa                                 <  1:7523310/94‑1 (MQ=255)
                                                         aaCGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATa                                 <  1:3871227/94‑1 (MQ=255)
                                                           cGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGc                               <  2:2162180/94‑1 (MQ=255)
                                                           cGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGc                               >  2:10314906/1‑94 (MQ=255)
                                                            ggTCTCGATACAGTGATTGGGGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTCTTGCGTGATAGcc                              >  2:6056393/1‑94 (MQ=255)
                                                               ctcGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCgt                           >  1:10625753/1‑93 (MQ=255)
                                                               ctcGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCga                           >  1:5241848/1‑94 (MQ=255)
                                                                 cGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCgatt                         >  2:1441311/1‑94 (MQ=255)
                                                                 cGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCgatt                         <  2:6548274/94‑1 (MQ=255)
                                                                   aTACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCgattct                       <  1:4539168/94‑1 (MQ=255)
                                                                   aTACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCgattct                       <  1:727680/94‑1 (MQ=255)
                                                                   aTACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCgattct                       >  1:1303365/1‑94 (MQ=255)
                                                                    tACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCgattctg                      <  1:4037279/94‑1 (MQ=255)
                                                                     aCAGTGATTGGTGAAAACGGCGTGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCgattctga                     >  2:10369360/1‑94 (MQ=255)
                                                                     aCAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCgattctga                     >  2:1258977/1‑94 (MQ=255)
                                                                      cAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCgattctgat                    >  1:6292432/1‑94 (MQ=255)
                                                                      cAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCgattctgat                    >  2:955331/1‑94 (MQ=255)
                                                                      cAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCgattctgat                    <  2:3459245/94‑1 (MQ=255)
                                                                      cAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCgattctgat                    >  1:6456104/1‑94 (MQ=255)
                                                                         tGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCgattctgattct                 >  1:7711712/1‑94 (MQ=255)
                                                                           aTTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCTgg               <  1:5815001/94‑1 (MQ=255)
                                                                            ttGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCTGGa              <  1:2355894/94‑1 (MQ=255)
                                                                             tGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCTGGAc             >  2:3190594/1‑94 (MQ=255)
                                                                             tGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCTGGAc             <  2:8301563/94‑1 (MQ=255)
                                                                             tGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCTGGAc             <  1:1277147/94‑1 (MQ=255)
                                                                              ggTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCTGGACg            >  2:5629993/1‑94 (MQ=255)
                                                                                 gAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCTGGACGAAg         >  1:2215159/1‑94 (MQ=255)
                                                                                 gAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCTGGACGAAg         >  1:10402909/1‑94 (MQ=255)
                                                                                  aaaaCGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCTGGACGAAGc        >  2:3406009/1‑94 (MQ=255)
                                                                                  aaaaCGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCTGGACGAAGc        >  1:9297881/1‑94 (MQ=255)
                                                                                    aaCGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCGCGATTCTGATTCTGGACGAAGCTa      >  1:9581168/1‑94 (MQ=255)
                                                                                     aCGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCTGGACGACGCTAc     >  1:921671/1‑94 (MQ=255)
                                                                                        gcgcgcTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTTCGTGATAGCCCGATTCTGATTCTGGACGAAGCTACCTc  <  2:5233877/94‑1 (MQ=255)
                                                                                        gcgcgcTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCTGGACGAAGCTACCTc  <  1:5769472/94‑1 (MQ=255)
                                                                                        gcgcgcTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCTGGACGAAGCTACCTc  <  2:3720794/94‑1 (MQ=255)
                                                                                        gcgcgcTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCTGGACGAAGCTACCTc  <  1:2095673/94‑1 (MQ=255)
                                                                                        gcgcgcTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCTGGACGAAGCTACCTc  <  2:7974826/94‑1 (MQ=255)
                                                                                           |                                                                                          
CAAATTGAAGAAGCGGCGCGTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGTGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCTGGACGAAGCTACCTC  >  NC_000913/967188‑967369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCAAATTGAAGAAGCGGCGCGTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGTGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCTGGACGAAGCTACCTCGGC  >  NC_000913/967186‑967372
                                                                                             |                                                                                             
AGCAAATTGAAGAAGCGGCGCGTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGC                                                                                               <  SRR4302545.4722284/94‑1 (MQ=60)
 GCAAATTGAAGAAGCGGCGCGTATGGACTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACAGCGCG                                                                                              >  SRR4302545.3134760/1‑94 (MQ=60)
   AAATTGAAGAAGCGGCGCGTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCT                                                                                            <  SRR4302545.2517281/94‑1 (MQ=60)
   AAATTGAAGAAGCGGCGCGTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCT                                                                                            <  SRR4302545.3563715/94‑1 (MQ=60)
   AAATTGAAGAAGCGGCGCGTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCT                                                                                            <  SRR4302545.3563716/94‑1 (MQ=60)
   AAATTGAAGAAGCGGCGCGTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCT                                                                                            >  SRR4302545.4917612/1‑94 (MQ=60)
   AAATTGAAGAAGCGGCGCGTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCT                                                                                            <  SRR4302545.10824447/94‑1 (MQ=60)
   AAATTGAAGAAGCGGCGCGTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCT                                                                                            >  SRR4302545.10890957/1‑94 (MQ=60)
   AAATTGAAGAAGCGGCGCGTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCT                                                                                            <  SRR4302545.4514806/94‑1 (MQ=60)
      TTGAAGAAGCGGCGCGTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCT                                                                                         <  SRR4302545.2899382/94‑1 (MQ=60)
      TTGAAGAAGCGGCGCGTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCT                                                                                         >  SRR4302545.1777733/1‑94 (MQ=60)
       TGAAGAAGCGGCGCGTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTC                                                                                        <  SRR4302545.6440044/94‑1 (MQ=60)
          AGAAGCGGCGCGTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCT                                                                                     >  SRR4302545.5399254/1‑94 (MQ=60)
          AGAAGCGGCGCGTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCT                                                                                     <  SRR4302545.10747171/94‑1 (MQ=60)
           GAAGCGGCGCGTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTG                                                                                    <  SRR4302545.5327765/94‑1 (MQ=60)
           GAAGCGGCGCGTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTG                                                                                    >  SRR4302545.6829095/1‑94 (MQ=60)
           GAAGCGGCGCGTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTG                                                                                    >  SRR4302545.6829096/1‑94 (MQ=60)
            aagctgttcgtttggcctgcggcgTGGACTTCATCAATAAGATGGATAGCGGTCTTGATACGGTGATTGGTGAAAACGGGGCGCTGCTCTCTGG                                                                                   <  SRR4302545.440004/70‑1 (MQ=58)
            AAGCGGCGCGTATGACCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAATCGGCGCGCTGCTCTCTGG                                                                                   <  SRR4302545.440005/94‑1 (MQ=60)
             AGCGGCGCGTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGG                                                                                  >  SRR4302545.9592134/1‑94 (MQ=60)
             AGCGGCGCGTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGC                                                                                  <  SRR4302545.5788874/94‑1 (MQ=60)
             AGCGGCGCGTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGC                                                                                  >  SRR4302545.10913757/1‑94 (MQ=60)
              GCGGCGCGTATGGCCTACGCCATCGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCG                                                                                 <  SRR4302545.5965921/94‑1 (MQ=60)
               CGGCGCGTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGG                                                                                <  SRR4302545.117125/94‑1 (MQ=60)
               CGGCGCGTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGG                                                                                >  SRR4302545.2660153/1‑94 (MQ=60)
                GGCGCGTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAGACGGCGCGCTGATCTCTGGCGGT                                                                               >  SRR4302545.7391345/1‑94 (MQ=60)
                 GCGCGTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTC                                                                              <  SRR4302545.4168924/94‑1 (MQ=60)
                 GCGCGTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTC                                                                              >  SRR4302545.3872496/1‑94 (MQ=60)
                 GCGCGTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTC                                                                              <  SRR4302545.7374503/94‑1 (MQ=60)
                   GCGTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAG                                                                            >  SRR4302545.2833997/1‑94 (MQ=60)
                    CGTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGC                                                                           >  SRR4302545.4062628/1‑94 (MQ=60)
                     GTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCG                                                                          >  SRR4302545.1236862/1‑94 (MQ=60)
                     GTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCG                                                                          <  SRR4302545.653696/94‑1 (MQ=60)
                     GTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCG                                                                          >  SRR4302545.7118103/1‑94 (MQ=60)
                     GTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCG                                                                          <  SRR4302545.7354671/94‑1 (MQ=60)
                     GTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCG                                                                          <  SRR4302545.7354672/94‑1 (MQ=60)
                      TATGGCCTATGCCATGGTCTTCATCAATAAGATTGATAACGGTCTTGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGT                                                                         >  SRR4302545.9952185/1‑94 (MQ=60)
                      TATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGT                                                                         <  SRR4302545.8678303/94‑1 (MQ=60)
                      TATGGCCTACGCAATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGT                                                                         >  SRR4302545.6205013/1‑94 (MQ=60)
                        TGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCA                                                                       <  SRR4302545.179732/94‑1 (MQ=60)
                           CCTACGCCATGGACTTCATCAATAAGATGGGTAACGGTCTCGATACAGTGATTGGTGAAAACGGCGTGCTGCTCTCTGGTGGTCAccgtgacgg                                                                    >  SRR4302545.10707366/1‑85 (MQ=60)
                            CTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGT                                                                   >  SRR4302545.9883406/1‑94 (MQ=60)
                            CTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCCCTCTGGCGGTCAGCGTCAGCGT                                                                   <  SRR4302545.8417688/94‑1 (MQ=60)
                             TACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTA                                                                  >  SRR4302545.8755825/1‑94 (MQ=60)
                              ACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTAT                                                                 <  SRR4302545.3919868/94‑1 (MQ=60)
                               CGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATT                                                                >  SRR4302545.3631283/1‑94 (MQ=60)
                                 TCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGC                                                              <  SRR4302545.3626052/94‑1 (MQ=60)
                                 CCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGC                                                              <  SRR4302545.1578495/94‑1 (MQ=60)
                                 CCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGC                                                              >  SRR4302545.5044014/1‑94 (MQ=60)
                                 CCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGC                                                              >  SRR4302545.8884134/1‑94 (MQ=60)
                                  CATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAACACGGAGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCT                                                             >  SRR4302545.10250176/1‑94 (MQ=60)
                                  CATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCT                                                             <  SRR4302545.8272220/94‑1 (MQ=60)
                                   ATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTA                                                            >  SRR4302545.7236977/1‑94 (MQ=60)
                                   ATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTA                                                            >  SRR4302545.7236974/1‑94 (MQ=60)
                                    TGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTAT                                                           <  SRR4302545.9711660/94‑1 (MQ=60)
                                     TGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATC                                                          <  SRR4302545.5502951/94‑1 (MQ=60)
                                     GGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATC                                                          <  SRR4302545.10421565/94‑1 (MQ=60)
                                     GGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATC                                                          <  SRR4302545.4964793/94‑1 (MQ=60)
                                      GACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCG                                                         >  SRR4302545.5149490/1‑94 (MQ=60)
                                      GACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCG                                                         >  SRR4302545.5149489/1‑94 (MQ=60)
                                        CTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTTAAAACGTCGCGCTGCTCTATGGCGGTTAGCCTCAGCGTATTGCTATCGCT                                                       >  SRR4302545.1223566/1‑94 (MQ=60)
                                        CTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCT                                                       >  SRR4302545.3716164/1‑94 (MQ=60)
                                        CTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCT                                                       >  SRR4302545.8439386/1‑94 (MQ=60)
                                           CATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGA                                                    >  SRR4302545.9213846/1‑94 (MQ=60)
                                           CATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGA                                                    >  SRR4302545.6016476/1‑94 (MQ=60)
                                            ATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAG                                                   <  SRR4302545.6284407/94‑1 (MQ=60)
                                            ATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAG                                                   <  SRR4302545.973889/94‑1 (MQ=60)
                                            ATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCGCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAG                                                   <  SRR4302545.5766406/94‑1 (MQ=60)
                                             TCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGC                                                  >  SRR4302545.361964/1‑94 (MQ=60)
                                             TCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGC                                                  >  SRR4302545.5416437/1‑94 (MQ=60)
                                             TCAATAAGATAGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGC                                                  <  SRR4302545.4463907/94‑1 (MQ=60)
                                               AATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCT                                                >  SRR4302545.10632137/1‑94 (MQ=60)
                                               AATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCT                                                <  SRR4302545.9790621/94‑1 (MQ=60)
                                                ATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTT                                               >  SRR4302545.4463628/1‑94 (MQ=60)
                                                 TAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTG                                              <  SRR4302545.5604860/94‑1 (MQ=60)
                                                  AAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGT                                             >  SRR4302545.4713867/1‑94 (MQ=60)
                                                   ATATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGtcgttgt                                            >  SRR4302545.9672195/1‑87 (MQ=60)
                                                   AGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTT                                            <  SRR4302545.10501964/94‑1 (MQ=60)
                                                   AGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTT                                            >  SRR4302545.1486125/1‑94 (MQ=60)
                                                   AGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTT                                            <  SRR4302545.4896615/94‑1 (MQ=60)
                                                    GATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTG                                           >  SRR4302545.2285045/1‑94 (MQ=60)
                                                     ATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGC                                          >  SRR4302545.2950103/1‑94 (MQ=60)
                                                     ATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGC                                          <  SRR4302545.10479946/94‑1 (MQ=60)
                                                     AGGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGC                                          <  SRR4302545.6666146/94‑1 (MQ=60)
                                                       GGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGT                                        <  SRR4302545.5784052/94‑1 (MQ=60)
                                                        GATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTG                                       >  SRR4302545.3346382/1‑94 (MQ=60)
                                                         ATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGA                                      >  SRR4302545.9537663/1‑94 (MQ=60)
                                                         ATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGA                                      <  SRR4302545.8893820/94‑1 (MQ=60)
                                                         ATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGA                                      <  SRR4302545.2385747/94‑1 (MQ=60)
                                                           AACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATA                                    <  SRR4302545.7736146/94‑1 (MQ=60)
                                                           AACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATA                                    <  SRR4302545.3967861/94‑1 (MQ=60)
                                                            ACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAG                                   <  SRR4302545.7726389/94‑1 (MQ=60)
                                                            ACGGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAG                                   <  SRR4302545.8954705/94‑1 (MQ=60)
                                                              GGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCC                                 >  SRR4302545.1779234/1‑94 (MQ=60)
                                                              GGTCTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCC                                 >  SRR4302545.9832048/1‑94 (MQ=60)
                                                                 CTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGT                              >  SRR4302545.10938560/1‑94 (MQ=60)
                                                                 CTCGATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGA                              >  SRR4302545.5377142/1‑94 (MQ=60)
                                                                    GATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTC                           >  SRR4302545.7760502/1‑94 (MQ=60)
                                                                     ATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCT                          >  SRR4302545.3262669/1‑94 (MQ=60)
                                                                     ATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCT                          <  SRR4302545.4655523/94‑1 (MQ=60)
                                                                     ATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCT                          <  SRR4302545.2641134/94‑1 (MQ=60)
                                                                     ATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCT                          <  SRR4302545.514957/94‑1 (MQ=60)
                                                                     ATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCT                          <  SRR4302545.744057/94‑1 (MQ=60)
                                                                     ATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCT                          >  SRR4302545.1332305/1‑94 (MQ=60)
                                                                     ATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCT                          >  SRR4302545.10477287/1‑94 (MQ=60)
                                                                     ATACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCT                          <  SRR4302545.4394726/94‑1 (MQ=60)
                                                                      TACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTG                         <  SRR4302545.4138362/94‑1 (MQ=60)
                                                                      TACAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTG                         <  SRR4302545.3759777/94‑1 (MQ=60)
                                                                        CAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGAT                       <  SRR4302545.2897354/94‑1 (MQ=60)
                                                                        CAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGAT                       >  SRR4302545.6463999/1‑94 (MQ=60)
                                                                        CAGTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGAT                       >  SRR4302545.6632619/1‑94 (MQ=60)
                                                                          GTGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTC                     >  SRR4302545.4415161/1‑94 (MQ=60)
                                                                           TGATTGGTGAAAACGGCGCTCTGCCCTCTGGCCGTCAGCGTCAGCGAATTGCTATCGCTCGAGCCTTGTTGCGTGATGGCGCGATTCTGATTCT                    <  SRR4302545.8417015/94‑1 (MQ=60)
                                                                           TGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCT                    <  SRR4302545.4721475/94‑1 (MQ=60)
                                                                           TGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCT                    >  SRR4302545.11027/1‑94 (MQ=60)
                                                                           TGATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCT                    >  SRR4302545.7930035/1‑94 (MQ=60)
                                                                           TAATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCT                    <  SRR4302545.2757996/94‑1 (MQ=60)
                                                                            GATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCTG                   <  SRR4302545.3935976/94‑1 (MQ=60)
                                                                            GATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGAGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCTG                   <  SRR4302545.8124718/94‑1 (MQ=60)
                                                                            GATTAGTGAAAACGGCGCGCCGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCTG                   <  SRR4302545.8051571/94‑1 (MQ=60)
                                                                             ATTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCTGG                  <  SRR4302545.5971455/94‑1 (MQ=60)
                                                                              TTGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCTGGA                 <  SRR4302545.2413193/94‑1 (MQ=60)
                                                                               TGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCTGGAC                <  SRR4302545.8319970/94‑1 (MQ=60)
                                                                               TGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCTGGAC                <  SRR4302545.1305635/94‑1 (MQ=60)
                                                                               TGGTGAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCTGGAC                <  SRR4302545.6365887/94‑1 (MQ=60)
                                                                               TGGTGAAAACGGCGCGCTCCTCGCTGGCGTTTAGCGTTAGCGTATTGCTAGCGTTCGAGCCTTGTTGCGTTATAGCCCGATTCTGATTCTGGAC                <  SRR4302545.8319971/94‑1 (MQ=53)
                                                                                   GAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCTGGACGAAG            >  SRR4302545.2269254/1‑94 (MQ=60)
                                                                                   GAAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCTGGACGAAG            >  SRR4302545.10709588/1‑94 (MQ=60)
                                                                                    AAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCTGGACGAAGC           <  SRR4302545.2214162/94‑1 (MQ=60)
                                                                                    AAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCTGGACGAAGC           >  SRR4302545.1256603/1‑94 (MQ=60)
                                                                                    AAAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCTGGACGAAGC           >  SRR4302545.9570466/1‑94 (MQ=60)
                                                                                     AAACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCTGGACGAAGCT          <  SRR4302545.4112420/94‑1 (MQ=60)
                                                                                      AACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCGCGATTCTGATTCTGGACGAAGCTA         >  SRR4302545.9863538/1‑94 (MQ=60)
                                                                                      AACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCTGGACGAAGCTA         <  SRR4302545.8788927/94‑1 (MQ=60)
                                                                                      AACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCTGGACGAAGCTA         <  SRR4302545.1097132/94‑1 (MQ=60)
                                                                                       ACGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCTGGACGACGCTAC        >  SRR4302545.942359/1‑94 (MQ=60)
                                                                                        CGGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCTGGACGAAGCTACC       <  SRR4302545.75618/94‑1 (MQ=60)
                                                                                         GGCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCTGGACGAAGCTACCT      <  SRR4302545.1331748/94‑1 (MQ=60)
                                                                                          GCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCTGGACGAAGCTACCTC     <  SRR4302545.5924517/94‑1 (MQ=60)
                                                                                          GCGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCTGGACGAAGCTACCTC     <  SRR4302545.2147203/94‑1 (MQ=60)
                                                                                           CGCGCTGCTCTGTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCTGGACGAAGCTACCTCG    >  SRR4302545.7051114/1‑94 (MQ=60)
                                                                                           CGCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCTGGACGAAGCTACCTCG    <  SRR4302545.726771/94‑1 (MQ=60)
                                                                                           CGCGCTCCTCTCTGGCGGTCAGCGTCAGCGTATTGCTAGCGCGCGATCCTTGTTGCGTGATAGCCCGATTCTGATTCTGGACGAAGCTACCTCG    <  SRR4302545.3298131/94‑1 (MQ=60)
                                                                                            GCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCTGGACGAAGCTACCTCGG   >  SRR4302545.7824956/1‑94 (MQ=60)
                                                                                            GCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCTGGACGAAGCTACCTCGG   <  SRR4302545.486025/94‑1 (MQ=60)
                                                                                            GCGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCTGGACGAAGCTACCTCGG   >  SRR4302545.9893892/1‑94 (MQ=60)
                                                                                             CGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCTGGACGAAGCTACCTCGGC  <  SRR4302545.5810639/94‑1 (MQ=60)
                                                                                             |                                                                                             
AGCAAATTGAAGAAGCGGCGCGTATGGCCTACGCCATGGACTTCATCAATAAGATGGATAACGGTCTCGATACAGTGATTGGTGAAAACGGCGTGCTGCTCTCTGGCGGTCAGCGTCAGCGTATTGCTATCGCTCGAGCCTTGTTGCGTGATAGCCCGATTCTGATTCTGGACGAAGCTACCTCGGC  >  NC_000913/967186‑967372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑