Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 971,671 T→C F233L (TTC→CTC)  b0919 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913971,6710TC99.2% 925.9 / ‑4.3 268F233L (TTC→CTC) b0919hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base C (148/117);  total (151/117)
Fisher's exact test for biased strand distribution p-value = 5.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

Only 100 of 274 total aligned reads displayed.

TACGCAGCATGTTGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGTTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCATCTGGCGGCGTT  >  NC_000913/971579‑971761
                                                                                            |                                                                                          
tACACAGCATGTTGAAAGATACGTGCAGAGATCAGTTACAGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGtt                                                                                           <  1:9084644/94‑1 (MQ=255)
  cGCAGCATGTTGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTcc                                                                                         <  2:2697929/94‑1 (MQ=255)
  cGCAGCATGTTGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTcc                                                                                         <  1:825545/94‑1 (MQ=255)
    cagcaTGTTGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCgc                                                                                       >  1:5249423/1‑93 (MQ=255)
    cagcaTGTTGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGa                                                                                       <  1:6576697/94‑1 (MQ=255)
    cagcaTGTTGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGa                                                                                       >  2:2416578/1‑94 (MQ=255)
    cagcaTGTTGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGa                                                                                       <  1:8664570/94‑1 (MQ=255)
       caTGTTGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTa                                                                                    >  1:2509768/1‑94 (MQ=255)
        aTGTTGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTaa                                                                                   >  1:437297/1‑94 (MQ=255)
        aTGTTGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTaa                                                                                   >  1:3381877/1‑94 (MQ=255)
        aTGTTGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTaa                                                                                   >  1:9666936/1‑94 (MQ=255)
        aTGTTGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTaa                                                                                   >  2:315108/1‑94 (MQ=255)
        aTGTTGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTaa                                                                                   >  2:6291074/1‑94 (MQ=255)
          gTTGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATg                                                                                 >  2:4201099/1‑94 (MQ=255)
            tGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGa                                                                               >  1:8146992/1‑94 (MQ=255)
            tGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGa                                                                               >  1:4877034/1‑94 (MQ=255)
             gAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGAt                                                                              <  1:8856652/94‑1 (MQ=255)
              aaaGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATa                                                                             >  1:154977/1‑94 (MQ=255)
               aaGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATaa                                                                            >  1:5180409/1‑94 (MQ=255)
                aGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAAt                                                                           <  2:7526617/94‑1 (MQ=255)
                 gATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAAtt                                                                          >  2:860163/1‑94 (MQ=255)
                    tCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATtctc                                                                       >  2:4299318/1‑94 (MQ=255)
                     cgcgCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTctct                                                                      <  1:7689393/94‑1 (MQ=255)
                     cgcgCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTctct                                                                      <  1:5873575/94‑1 (MQ=255)
                      gcgcAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTg                                                                     <  2:5579373/94‑1 (MQ=255)
                       cgcAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTgg                                                                    >  1:10243121/1‑94 (MQ=255)
                       cgcAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTgg                                                                    >  2:9853361/1‑94 (MQ=255)
                       cgcAGCGATCAGGTACTGGCGATGGTCGGGCCGGGACTAATGCTTTCGGCAACGCGAGAATACGAACTGTTCCGACTAATGGATAATTATCTgg                                                                    >  1:6918811/1‑94 (MQ=255)
                        gcAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGc                                                                   >  1:4095097/1‑94 (MQ=255)
                         cAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCg                                                                  >  1:9143681/1‑94 (MQ=255)
                             gATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAg                                                              <  1:4645299/94‑1 (MQ=255)
                             gATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAg                                                              >  2:7706661/1‑94 (MQ=255)
                             gATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAg                                                              >  1:10068825/1‑94 (MQ=255)
                              aTCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGa                                                             >  1:3909554/1‑94 (MQ=255)
                               tCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGAt                                                            <  2:3260141/94‑1 (MQ=255)
                                cAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGAtt                                                           >  2:2138096/1‑94 (MQ=255)
                                cAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGCGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGAtt                                                           >  1:7406213/1‑94 (MQ=255)
                                  gTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGTACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTg                                                         >  2:2869283/1‑94 (MQ=255)
                                  gTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTg                                                         <  1:2137478/94‑1 (MQ=255)
                                    tACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGct                                                       <  1:950359/94‑1 (MQ=255)
                                       tGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTg                                                    <  2:6724264/94‑1 (MQ=255)
                                       tGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTg                                                    <  2:1161272/94‑1 (MQ=255)
                                        ggCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTgg                                                   >  2:6402789/1‑94 (MQ=255)
                                         gCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGa                                                  <  1:1901222/94‑1 (MQ=255)
                                           gATGGTCGGGCCGGTGCTAATGCTGTGGGCACCGTGAGAATACGAACTGCTGCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGt                                                <  2:8659076/94‑1 (MQ=255)
                                             tGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTa                                              <  1:7288570/94‑1 (MQ=255)
                                             tGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTa                                              >  2:8766840/1‑94 (MQ=255)
                                              ggTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTAc                                             >  2:9105082/1‑94 (MQ=255)
                                               gTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTAcc                                            >  1:2447016/1‑94 (MQ=255)
                                               gTCGGACCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTAcc                                            >  1:3172679/1‑94 (MQ=255)
                                                tCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCt                                           <  1:1443706/94‑1 (MQ=255)
                                                tCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCt                                           <  2:4613850/94‑1 (MQ=255)
                                                tCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCt                                           <  2:4924336/94‑1 (MQ=255)
                                                 cGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTg                                          <  1:6263183/94‑1 (MQ=255)
                                                   ggCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCa                                        <  2:8386577/94‑1 (MQ=255)
                                                    gCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCaa                                       >  1:6744485/1‑94 (MQ=255)
                                                      cGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAAcg                                     <  2:5497717/94‑1 (MQ=255)
                                                      cGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAAcg                                     <  2:163337/94‑1 (MQ=255)
                                                      cGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAAcg                                     <  2:4470122/94‑1 (MQ=255)
                                                      cGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAAcg                                     >  1:7146950/1‑94 (MQ=255)
                                                      cGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAAcg                                     <  1:10398655/94‑1 (MQ=255)
                                                      cGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAAcg                                     >  1:560169/1‑94 (MQ=255)
                                                       gggACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAAcgc                                    >  2:9332688/1‑94 (MQ=255)
                                                       gggACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAAcgc                                    >  1:6062394/1‑94 (MQ=255)
                                                       gggACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAAcgc                                    >  1:1234939/1‑94 (MQ=255)
                                                       gggACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAAcgc                                    >  2:2090716/1‑94 (MQ=255)
                                                        ggACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAAcgcg                                   >  2:3461553/1‑94 (MQ=255)
                                                         gACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAAcgcgc                                  <  2:5517132/94‑1 (MQ=255)
                                                           cTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACgcgcgc                                >  2:515983/1‑94 (MQ=255)
                                                            tAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGcc                               >  1:3064318/1‑94 (MQ=255)
                                                             aaTGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCa                              >  2:1338730/1‑94 (MQ=255)
                                                              aTGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAg                             <  1:2229551/94‑1 (MQ=255)
                                                               tGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGt                            >  2:9568638/1‑94 (MQ=255)
                                                                 cTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTgg                          <  2:4332571/94‑1 (MQ=255)
                                                                  tttGGGCACCGCGAGAATACGAACTGCTCCGACTCATGGATAATTCTCTGGCGGAAGAGTTGCTCTGCAGTTACCTGCAAAGCGCGCCAGTGGc                         >  2:8210576/1‑94 (MQ=255)
                                                                  tttGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGc                         <  2:1055843/94‑1 (MQ=255)
                                                                    tGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCgg                       >  2:5850692/1‑94 (MQ=255)
                                                                    tGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCgg                       <  1:2709291/94‑1 (MQ=255)
                                                                     gggCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCACCAGTGGCGGa                      >  2:3983642/1‑94 (MQ=255)
                                                                      gtcACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAg                     <  1:6633907/92‑1 (MQ=255)
                                                                        cACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGtg                   >  2:7824342/1‑93 (MQ=255)
                                                                        cACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTc                   <  1:3529427/94‑1 (MQ=255)
                                                                         aCCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCg                  >  1:1649127/1‑94 (MQ=255)
                                                                           cgcgAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGtt                <  2:2767302/94‑1 (MQ=255)
                                                                             cgAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCa              <  1:10078943/94‑1 (MQ=255)
                                                                             cgAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCa              >  1:5003463/1‑94 (MQ=255)
                                                                              gagaATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCAt             <  2:8269208/94‑1 (MQ=255)
                                                                              gagaATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCAt             >  1:10359172/1‑94 (MQ=255)
                                                                                 aaTACGAACTGCTCCGACTAATGGATAATTCTCTGGCGTAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTAGTTCATCTg          >  2:1767272/1‑94 (MQ=255)
                                                                                 aaTACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCATCTg          <  2:6180839/94‑1 (MQ=255)
                                                                                 aaTACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCATCTg          <  2:4690313/94‑1 (MQ=255)
                                                                                 aaTACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCATCTg          >  1:4568821/1‑94 (MQ=255)
                                                                                  aTACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCATCTgg         <  2:7305900/94‑1 (MQ=255)
                                                                                  aTACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCATCTgg         >  2:2491321/1‑94 (MQ=255)
                                                                                  aTACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCATCTgg         >  1:6458928/1‑94 (MQ=255)
                                                                                  aTACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGGCGTTCCTCTgg         >  2:10261993/1‑94 (MQ=255)
                                                                                    aCGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCATCTggcg       <  2:846059/94‑1 (MQ=255)
                                                                                     cGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCATCTggcgg      >  2:9715792/1‑94 (MQ=255)
                                                                                       aaCTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGCTCATCTtgcctcc    >  1:6071246/1‑90 (MQ=255)
                                                                                         cTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCATCTGGCGGCgtt  >  2:10144671/1‑94 (MQ=255)
                                                                                            |                                                                                          
TACGCAGCATGTTGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGTTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCATCTGGCGGCGTT  >  NC_000913/971579‑971761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTACGCAGCATGTTGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGTTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCATCTGGCGGCGTTGGT  >  NC_000913/971578‑971764
                                                                                             |                                                                                             
TTACGCAGCATGTTGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGC                                                                                               >  SRR4302545.4271806/1‑94 (MQ=60)
TTACGCAGCATGTTGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGC                                                                                               >  SRR4302545.10502421/1‑94 (MQ=60)
 TACGCAGCATGTTGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCT                                                                                              >  SRR4302545.8457457/1‑94 (MQ=60)
 TACACAGCATGTTGAAAGATACGTGCAGAGATCAGTTACAGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGTT                                                                                              <  SRR4302545.9350437/94‑1 (MQ=60)
   CGCAGCATGTTGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCC                                                                                            <  SRR4302545.844056/94‑1 (MQ=60)
   CGCAGCATGTTGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCC                                                                                            <  SRR4302545.2107223/94‑1 (MQ=60)
   CGCAGCATGTTGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCC                                                                                            <  SRR4302545.2271043/94‑1 (MQ=60)
   CGCAGCATGTTGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCC                                                                                            >  SRR4302545.1292083/1‑94 (MQ=60)
   CGCAGCATGTTGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCC                                                                                            >  SRR4302545.2878925/1‑94 (MQ=60)
    GCAGCATGTTGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCG                                                                                           >  SRR4302545.10874139/1‑94 (MQ=60)
     CAGCATGTTGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGC                                                                                          >  SRR4302545.5385009/1‑94 (MQ=60)
     CAGCATGTTGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGA                                                                                          <  SRR4302545.6756687/94‑1 (MQ=60)
     CAGCATGTTGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGA                                                                                          <  SRR4302545.8915905/94‑1 (MQ=60)
     CAGCATGTTGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGA                                                                                          >  SRR4302545.10650961/1‑94 (MQ=60)
        CATGTTGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTA                                                                                       >  SRR4302545.2570441/1‑94 (MQ=60)
         ATGTTGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAA                                                                                      >  SRR4302545.9953563/1‑94 (MQ=60)
         ATGTTGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAA                                                                                      >  SRR4302545.3464298/1‑94 (MQ=60)
         ATGTTGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAA                                                                                      >  SRR4302545.6506210/1‑94 (MQ=60)
         ATGTTGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAA                                                                                      >  SRR4302545.10494475/1‑94 (MQ=60)
         ATGTTGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAA                                                                                      >  SRR4302545.4165214/1‑94 (MQ=60)
         ATGTTGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAA                                                                                      >  SRR4302545.447023/1‑94 (MQ=60)
          TGTTGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAAT                                                                                     >  SRR4302545.6868672/1‑94 (MQ=60)
          TGTTGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAAT                                                                                     <  SRR4302545.5007766/94‑1 (MQ=60)
          TGTTGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAAT                                                                                     >  SRR4302545.9486402/1‑94 (MQ=60)
           GTTGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATG                                                                                    >  SRR4302545.7978428/1‑94 (MQ=60)
           GTTGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATG                                                                                    <  SRR4302545.8772098/94‑1 (MQ=60)
           GTTGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATG                                                                                    <  SRR4302545.13241/94‑1 (MQ=60)
            TTGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGG                                                                                   <  SRR4302545.6151321/94‑1 (MQ=60)
             TGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGA                                                                                  >  SRR4302545.8382402/1‑94 (MQ=60)
             TGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGA                                                                                  >  SRR4302545.5001046/1‑94 (MQ=60)
             TGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGA                                                                                  >  SRR4302545.6190825/1‑94 (MQ=60)
              GAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGAT                                                                                 >  SRR4302545.3586299/1‑94 (MQ=60)
              GAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGAT                                                                                 <  SRR4302545.9113916/94‑1 (MQ=60)
               AAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATA                                                                                >  SRR4302545.158340/1‑94 (MQ=60)
               AAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATA                                                                                >  SRR4302545.2524911/1‑94 (MQ=60)
               AAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATA                                                                                <  SRR4302545.6979741/94‑1 (MQ=60)
                AAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAA                                                                               >  SRR4302545.5313771/1‑94 (MQ=60)
                  GATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATT                                                                             <  SRR4302545.5276002/94‑1 (MQ=60)
                     TCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTC                                                                          >  SRR4302545.1032613/1‑94 (MQ=60)
                      CGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCT                                                                         <  SRR4302545.6031888/94‑1 (MQ=60)
                      CGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCT                                                                         <  SRR4302545.7310362/94‑1 (MQ=60)
                      CGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCT                                                                         <  SRR4302545.7907111/94‑1 (MQ=60)
                       GCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTG                                                                        <  SRR4302545.1808675/94‑1 (MQ=60)
                       GCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTG                                                                        <  SRR4302545.8327161/94‑1 (MQ=60)
                        CGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGG                                                                       >  SRR4302545.10545536/1‑94 (MQ=60)
                        CGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGG                                                                       <  SRR4302545.468389/94‑1 (MQ=60)
                        CGCAGCGATCAGGTACTGGCGATGGTCGGGCCGGGACTAATGCTTTCGGCAACGCGAGAATACGAACTGTTCCGACTAATGGATAATTATCTGG                                                                       >  SRR4302545.7109440/1‑94 (MQ=60)
                         GCAGCGATCAGTTACTGGCGTTGGCCTGTCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACAGCTCCGACTAATTGATAATTATCTGGG                                                                      >  SRR4302545.7619218/1‑94 (MQ=60)
                         GCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGC                                                                      >  SRR4302545.6898679/1‑94 (MQ=60)
                         GCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGC                                                                      >  SRR4302545.4197631/1‑94 (MQ=60)
                          CAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCG                                                                     >  SRR4302545.9412160/1‑94 (MQ=60)
                          CAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCG                                                                     >  SRR4302545.6805156/1‑94 (MQ=60)
                           AGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGG                                                                    >  SRR4302545.7735151/1‑94 (MQ=60)
                           AGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGG                                                                    <  SRR4302545.5527330/94‑1 (MQ=60)
                           AGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGG                                                                    >  SRR4302545.147927/1‑94 (MQ=60)
                              GATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAG                                                                 <  SRR4302545.4763971/94‑1 (MQ=60)
                              GATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAG                                                                 >  SRR4302545.10366770/1‑94 (MQ=60)
                              GATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAG                                                                 >  SRR4302545.10366767/1‑94 (MQ=60)
                               ATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGA                                                                >  SRR4302545.4007138/1‑94 (MQ=60)
                                 CAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGCGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATT                                                              >  SRR4302545.7615441/1‑94 (MQ=60)
                                  AGTTACTGGCGATGGTCGGGCCGGGCCTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTT                                                             >  SRR4302545.1012469/1‑94 (MQ=60)
                                  AGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGGTCCGACTAATGGATAATTCTCTGGCGGAAGATTT                                                             >  SRR4302545.9038967/1‑94 (MQ=60)
                                  AGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTT                                                             >  SRR4302545.4249674/1‑94 (MQ=60)
                                   GTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTG                                                            <  SRR4302545.2189852/94‑1 (MQ=60)
                                   GTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTG                                                            <  SRR4302545.3582726/94‑1 (MQ=60)
                                   GTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTG                                                            <  SRR4302545.364642/94‑1 (MQ=60)
                                    TTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGC                                                           >  SRR4302545.2604847/1‑94 (MQ=60)
                                     TACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCT                                                          <  SRR4302545.971705/94‑1 (MQ=60)
                                     TACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCT                                                          <  SRR4302545.2667015/94‑1 (MQ=60)
                                       CTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCT                                                        >  SRR4302545.771969/1‑94 (MQ=60)
                                        TGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTG                                                       >  SRR4302545.434876/1‑94 (MQ=60)
                                          GCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGA                                                     <  SRR4302545.1948144/94‑1 (MQ=60)
                                           CGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAG                                                    >  SRR4302545.6001390/1‑94 (MQ=60)
                                            GATTGTCGGGCCGGGGCTAATGCTTTTGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGT                                                   <  SRR4302545.9376011/94‑1 (MQ=60)
                                              TGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGTAAGATTTGCTCTGGAGTTA                                                 <  SRR4302545.10673891/94‑1 (MQ=60)
                                              TGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTA                                                 <  SRR4302545.7492978/94‑1 (MQ=60)
                                              TGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTA                                                 >  SRR4302545.2558394/1‑94 (MQ=60)
                                               GGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTAC                                                >  SRR4302545.4695878/1‑94 (MQ=60)
                                                GTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACC                                               >  SRR4302545.2506296/1‑94 (MQ=60)
                                                GTCGGACCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACC                                               >  SRR4302545.3250565/1‑94 (MQ=60)
                                                 TCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCT                                              <  SRR4302545.1476595/94‑1 (MQ=60)
                                                 GCGGGCCGGGACTAATGCTGTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGATTTACCT                                              <  SRR4302545.1476597/94‑1 (MQ=60)
                                                  ggggcagtgagTAATGCTTTGGGCACTGCGATAATACGAACTGCTCTGAATAATGGGTAATTCTCTGGCATAAGATTTGCTCTGGAGTTAGCTG                                             <  SRR4302545.5671002/83‑1 (MQ=35)
                                                  CGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTG                                             <  SRR4302545.10552195/94‑1 (MQ=60)
                                                  CGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTG                                             <  SRR4302545.6433940/94‑1 (MQ=60)
                                                  CGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTG                                             <  SRR4302545.5491914/94‑1 (MQ=60)
                                                   GGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGACGGAAGATTTGCTCTGGAGTTACCTGA                                            >  SRR4302545.10912770/1‑94 (MQ=60)
                                                    GGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCA                                           <  SRR4302545.8470896/94‑1 (MQ=60)
                                                    GGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCA                                           <  SRR4302545.9109549/94‑1 (MQ=60)
                                                     GCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAA                                          >  SRR4302545.6929423/1‑94 (MQ=60)
                                                      CCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAGGATTTGCTCTGGAGTTACCTGCAAC                                         <  SRR4302545.9759702/94‑1 (MQ=60)
                                                       CGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGTAACATTTGCTCGTGAGTTACCTGCGACG                                        >  SRR4302545.7345304/1‑94 (MQ=60)
                                                       CGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACG                                        >  SRR4302545.572725/1‑94 (MQ=60)
                                                       CGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACG                                        >  SRR4302545.7345305/1‑94 (MQ=60)
                                                       CGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACG                                        <  SRR4302545.10705214/94‑1 (MQ=60)
                                                        GGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGC                                       >  SRR4302545.6226919/1‑94 (MQ=60)
                                                        GGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGC                                       >  SRR4302545.6226918/1‑94 (MQ=60)
                                                        GGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGC                                       >  SRR4302545.1262815/1‑94 (MQ=60)
                                                        GGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGC                                       >  SRR4302545.1593123/1‑94 (MQ=60)
                                                         GGACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCG                                      >  SRR4302545.3301958/1‑94 (MQ=60)
                                                          GACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGC                                     >  SRR4302545.6386530/1‑94 (MQ=60)
                                                           ACTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCG                                    <  SRR4302545.658000/94‑1 (MQ=60)
                                                            CTAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGC                                   >  SRR4302545.63158/1‑94 (MQ=60)
                                                             TAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCC                                  >  SRR4302545.4573632/1‑94 (MQ=60)
                                                             TAATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCC                                  >  SRR4302545.3139454/1‑94 (MQ=60)
                                                              AATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCA                                 <  SRR4302545.9129039/94‑1 (MQ=60)
                                                               ATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAG                                >  SRR4302545.9135817/1‑94 (MQ=60)
                                                               ATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAG                                >  SRR4302545.10158031/1‑94 (MQ=60)
                                                               ATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAG                                >  SRR4302545.3009311/1‑94 (MQ=60)
                                                               ATGCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAG                                <  SRR4302545.2283952/94‑1 (MQ=60)
                                                                TGCTTTGGGCACCGCGAGAATACTAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGT                               <  SRR4302545.5963665/94‑1 (MQ=60)
                                                                 GCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTG                              >  SRR4302545.5049346/1‑94 (MQ=60)
                                                                 GCTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTG                              >  SRR4302545.9823169/1‑94 (MQ=60)
                                                                  CTTTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGCGG                             <  SRR4302545.7563860/94‑1 (MQ=60)
                                                                    TTGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCG                           <  SRR4302545.8576643/94‑1 (MQ=60)
                                                                     TGGGCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGG                          <  SRR4302545.2774565/94‑1 (MQ=60)
                                                                       GTCACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAG                        <  SRR4302545.6815724/94‑1 (MQ=60)
                                                                         CACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTC                      <  SRR4302545.3615925/94‑1 (MQ=60)
                                                                         CACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGGC                      >  SRR4302545.4847732/1‑94 (MQ=60)
                                                                          ACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCG                     <  SRR4302545.2166243/94‑1 (MQ=60)
                                                                          ACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCG                     >  SRR4302545.1688194/1‑94 (MQ=60)
                                                                          ACCGCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCG                     <  SRR4302545.3419803/94‑1 (MQ=60)
                                                                             GCGAGAATACGAACTGTTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGGCGTTC                  >  SRR4302545.5370450/1‑94 (MQ=60)
                                                                             GCGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTC                  <  SRR4302545.7092161/94‑1 (MQ=60)
                                                                              CGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCA                 <  SRR4302545.10377103/94‑1 (MQ=60)
                                                                              CGAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCA                 >  SRR4302545.5130234/1‑94 (MQ=60)
                                                                               GAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCAT                <  SRR4302545.453187/94‑1 (MQ=60)
                                                                               GAGAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCAT                >  SRR4302545.10664557/1‑94 (MQ=60)
                                                                                 GAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCATCT              >  SRR4302545.3938903/1‑94 (MQ=60)
                                                                                 GAATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCATCT              >  SRR4302545.988055/1‑94 (MQ=60)
                                                                                  AATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCATCTG             >  SRR4302545.4685982/1‑94 (MQ=60)
                                                                                   ATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCATCTGG            >  SRR4302545.6635518/1‑94 (MQ=60)
                                                                                   ATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCATCTGG            >  SRR4302545.6635517/1‑94 (MQ=60)
                                                                                   ATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCATCTGG            >  SRR4302545.6635516/1‑94 (MQ=60)
                                                                                   ATACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCATCTGG            >  SRR4302545.2312425/1‑94 (MQ=60)
                                                                                    TACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCATCTGGC           <  SRR4302545.1715038/94‑1 (MQ=60)
                                                                                     ACGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCATCTGGCG          <  SRR4302545.2242738/94‑1 (MQ=60)
                                                                                      CGAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCATCTGGCGG         <  SRR4302545.5206333/94‑1 (MQ=60)
                                                                                       GAACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGAGGCGGAGTCGTTCATCTGGCGGC        >  SRR4302545.7457907/1‑94 (MQ=60)
                                                                                        AACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCATCTGGCGGCG       >  SRR4302545.3240964/1‑94 (MQ=60)
                                                                                        AACTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGCTCATCTtgcctcc       >  SRR4302545.6236041/1‑87 (MQ=60)
                                                                                          CTGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCATCTGGCGGCGTT     >  SRR4302545.752988/1‑94 (MQ=60)
                                                                                           TGCTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCATCTGGCGGCGTTG    >  SRR4302545.5652508/1‑94 (MQ=60)
                                                                                             CTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCATCTGGCGGCGTTGGT  >  SRR4302545.3162560/1‑94 (MQ=60)
                                                                                             |                                                                                             
TTACGCAGCATGTTGAAAGATTCGCGCAGCGATCAGTTACTGGCGATGGTCGGGCCGGGACTAATGCTTTGGGCACCGCGAGAATACGAACTGTTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCATCTGGCGGCGTTGGT  >  NC_000913/971578‑971764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑