Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,953,793 T→C G413G (GGA→GGG)  b1872 ← biotin sulfoxide reductase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,953,7930TC98.9% 624.4 / ‑3.1 184G413G (GGA→GGG) b1872biotin sulfoxide reductase 2
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (80/102);  total (81/103)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

Only 100 of 194 total aligned reads displayed.

GATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGTCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCG  >  NC_000913/1953702‑1953883
                                                                                           |                                                                                          
gATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGccc                                                                                          <  2:5150819/94‑1 (MQ=255)
 atatTTGCCGCCAGGAGTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCg                                                                                         <  2:7868193/94‑1 (MQ=255)
  tatTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCgg                                                                                        <  1:8305049/94‑1 (MQ=255)
  tatTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCgg                                                                                        >  1:795663/1‑94 (MQ=255)
    tttGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCa                                                                                      >  2:9186504/1‑94 (MQ=255)
      tGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAAt                                                                                    <  2:467274/94‑1 (MQ=255)
       gccgccAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATc                                                                                   <  1:1330918/94‑1 (MQ=255)
       gccgccAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATc                                                                                   <  1:9740848/94‑1 (MQ=255)
       gccgccAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATc                                                                                   >  2:8024094/1‑94 (MQ=255)
         cctcAGGATGTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATcgc                                                                                 <  1:825676/91‑1 (MQ=255)
          gccAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATcgcc                                                                                <  2:8120349/94‑1 (MQ=255)
           ccAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATcgccg                                                                               >  1:321465/1‑94 (MQ=255)
           ccAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATcgccg                                                                               >  2:4838249/1‑94 (MQ=255)
           ccAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATcgccg                                                                               >  1:9332264/1‑94 (MQ=255)
            cAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATcgccgc                                                                              >  1:177426/1‑94 (MQ=255)
             aGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCa                                                                             >  1:4570520/1‑94 (MQ=255)
             aGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCa                                                                             >  2:9558332/1‑94 (MQ=255)
              ggATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAg                                                                            >  1:3192341/1‑94 (MQ=255)
              ggATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAg                                                                            <  2:6783275/94‑1 (MQ=255)
               gATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGa                                                                           >  1:8446998/1‑94 (MQ=255)
                aTTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGAc                                                                          <  2:5477432/94‑1 (MQ=255)
                aTTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGAc                                                                          <  1:999772/94‑1 (MQ=255)
                aTTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGAc                                                                          >  2:10188793/1‑94 (MQ=255)
                aTTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGAc                                                                          <  2:5702723/94‑1 (MQ=255)
                aTTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGAc                                                                          >  1:1935152/1‑94 (MQ=255)
                    tCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACAttt                                                                      <  1:10554543/94‑1 (MQ=255)
                     tcAGCGCATCGAAGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTc                                                                     <  2:3437767/93‑1 (MQ=255)
                     ccAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTc                                                                     <  2:6042077/94‑1 (MQ=255)
                     ccAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTc                                                                     >  2:8385868/1‑94 (MQ=255)
                      cggcgcATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTcc                                                                    <  2:352957/92‑1 (MQ=255)
                      cAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTcc                                                                    >  1:4961380/1‑94 (MQ=255)
                       aGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGTCCGGCAATCGCCGCAGACATTTCCg                                                                   >  1:5103498/1‑94 (MQ=255)
                        gcgcATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCgg                                                                  <  2:7146463/94‑1 (MQ=255)
                         cgcATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGgc                                                                 <  2:3873177/94‑1 (MQ=255)
                          gcATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGgca                                                                >  2:2308158/1‑94 (MQ=255)
                           cATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGgcag                                                               <  2:8678488/94‑1 (MQ=255)
                            aTCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGgcagc                                                              <  2:2533206/94‑1 (MQ=255)
                            aTCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGgcaga                                                              >  2:1299623/1‑93 (MQ=255)
                              cgacgaTACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCAc                                                            <  1:6109358/94‑1 (MQ=255)
                              cgacgaTACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCAc                                                            <  2:10539951/94‑1 (MQ=255)
                               cacgaTACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCAcg                                                           <  2:1536816/93‑1 (MQ=255)
                                acgaTACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCAcgc                                                          >  2:5809879/1‑94 (MQ=255)
                                 cgaTACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCAcgcc                                                         >  2:3001763/1‑94 (MQ=255)
                                  gagaCGGGCAACAGGGATCGCCGTCAGTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCAcgccg                                                        <  2:6263052/91‑1 (MQ=255)
                                  gaTACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCAcgccg                                                        >  1:2656925/1‑94 (MQ=255)
                                   aTACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCAcgccgc                                                       >  1:109550/1‑94 (MQ=255)
                                      cGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCaa                                                    >  2:848656/1‑94 (MQ=255)
                                       gggCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAac                                                   >  1:7478090/1‑94 (MQ=255)
                                         gCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAacac                                                 <  2:6839074/94‑1 (MQ=255)
                                         gCAACACGAATCGCTGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAacac                                                 <  1:5794/94‑1 (MQ=255)
                                          gaaCAGGAATCGGCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACAcg                                                <  1:8318193/93‑1 (MQ=255)
                                           aaCAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCCACAcgc                                               >  1:8185386/1‑94 (MQ=255)
                                             cAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGt                                             >  1:7318399/1‑94 (MQ=255)
                                             cAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGt                                             >  2:5202960/1‑94 (MQ=255)
                                              aGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTc                                            >  2:4324016/1‑94 (MQ=255)
                                                  aTCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGt                                        <  1:2421727/94‑1 (MQ=255)
                                                  aTCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGt                                        <  1:1241069/94‑1 (MQ=255)
                                                    cGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTg                                      >  2:3253653/1‑94 (MQ=255)
                                                     gCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGc                                     >  2:8914381/1‑94 (MQ=255)
                                                     gCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGc                                     >  2:1070409/1‑94 (MQ=255)
                                                     gCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGc                                     <  1:960813/94‑1 (MQ=255)
                                                        gTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCAc                                  >  1:10535487/1‑94 (MQ=255)
                                                         tCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCAcc                                 <  1:4854466/94‑1 (MQ=255)
                                                           aTTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGt                               >  1:991305/1‑94 (MQ=255)
                                                              ccGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTgg                            >  1:4471109/1‑94 (MQ=255)
                                                              ccGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTgg                            >  1:4471110/1‑94 (MQ=255)
                                                              ccGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTgg                            >  2:3626472/1‑94 (MQ=255)
                                                                 ccATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGt                         <  1:4828291/94‑1 (MQ=255)
                                                                 ccATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGt                         <  1:7629650/94‑1 (MQ=255)
                                                                 ccATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGt                         <  1:5428664/94‑1 (MQ=255)
                                                                  catcatCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTa                        <  1:5739849/94‑1 (MQ=255)
                                                                   atcatcAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTaa                       <  2:4570292/94‑1 (MQ=255)
                                                                   atcatcAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTaa                       <  2:4570290/94‑1 (MQ=255)
                                                                   atcatcAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTaa                       >  2:7758059/1‑94 (MQ=255)
                                                                    tcatcaGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAAt                      <  2:1874449/94‑1 (MQ=255)
                                                                     catcaGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATg                     <  2:6373703/94‑1 (MQ=255)
                                                                        caGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATa                  >  1:563803/1‑94 (MQ=255)
                                                                        caGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATa                  <  2:6223553/94‑1 (MQ=255)
                                                                         cgCGGCCTCGCTGTCATCCCCGGCAATCGCCGCATACATTTCCGGCAGCACCCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATaa                 <  1:3714969/93‑1 (MQ=255)
                                                                          gCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAag                <  2:4743180/94‑1 (MQ=255)
                                                                          gCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAag                <  2:8673073/94‑1 (MQ=255)
                                                                          gCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAag                <  2:1642691/94‑1 (MQ=255)
                                                                            ggCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAagag              <  1:3301132/94‑1 (MQ=255)
                                                                            ggCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAagag              >  2:9243494/1‑94 (MQ=255)
                                                                             gCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAgaga             <  1:8501480/94‑1 (MQ=255)
                                                                              ccTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGaa            >  1:8732428/1‑94 (MQ=255)
                                                                              ccTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGATTGCCACCGCTGGAGCAATGATAAGAGaa            >  1:4006693/1‑94 (MQ=255)
                                                                               cTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGaaa           <  1:9141571/94‑1 (MQ=255)
                                                                                tCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAAc          <  2:9001014/94‑1 (MQ=255)
                                                                                 cGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAAcc         <  2:82897/94‑1 (MQ=255)
                                                                                 cGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAAcc         >  2:9716823/1‑94 (MQ=255)
                                                                                  gCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCACCACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAACCCa        >  2:2643109/1‑94 (MQ=255)
                                                                                  gCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCa        >  2:5440197/1‑94 (MQ=255)
                                                                                   cTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCaa       >  1:5093116/1‑94 (MQ=255)
                                                                                    tGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCaaa      <  2:6954194/94‑1 (MQ=255)
                                                                                    cggCCTGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCaaa      <  2:4394545/93‑1 (MQ=255)
                                                                                     ggCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCaaaa     >  1:3869313/1‑94 (MQ=255)
                                                                                      gCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGGGTAATGATAAAAGAAACCAAAAc    >  1:602789/1‑94 (MQ=255)
                                                                                       cATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAAcc   <  2:176887/94‑1 (MQ=255)
                                                                                       aaTGCCCGGGAAGCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAAcc   <  1:2126004/93‑1 (MQ=255)
                                                                                           |                                                                                          
GATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGTCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCG  >  NC_000913/1953702‑1953883

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGTCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCG  >  NC_000913/1953700‑1953886
                                                                                             |                                                                                             
CTGATATTTGCCGCCAGGATTTTCAAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGC                                                                                               >  SRR4302545.7623357/1‑94 (MQ=60)
 TGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCC                                                                                              >  SRR4302545.2713204/1‑94 (MQ=60)
 TGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGAGGCCTCGCTGGCATGCC                                                                                              >  SRR4302545.6502963/1‑94 (MQ=60)
    TATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGG                                                                                           <  SRR4302545.8545661/94‑1 (MQ=60)
    TATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGG                                                                                           >  SRR4302545.813547/1‑94 (MQ=60)
         GCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATC                                                                                      <  SRR4302545.10030814/94‑1 (MQ=60)
         GCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATC                                                                                      <  SRR4302545.1360542/94‑1 (MQ=60)
         GCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATC                                                                                      <  SRR4302545.10030813/94‑1 (MQ=60)
           cctCAGGATGTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGC                                                                                    <  SRR4302545.844188/91‑1 (MQ=60)
            GCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCC                                                                                   >  SRR4302545.5105224/1‑94 (MQ=60)
            GCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCC                                                                                   >  SRR4302545.6125521/1‑94 (MQ=60)
             CCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCG                                                                                  >  SRR4302545.9605882/1‑94 (MQ=60)
             CCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCG                                                                                  >  SRR4302545.7707153/1‑94 (MQ=60)
             CCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCG                                                                                  >  SRR4302545.328286/1‑94 (MQ=60)
              CAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATTGCCGGCAATCGCCGC                                                                                 >  SRR4302545.1635737/1‑94 (MQ=60)
              CAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGC                                                                                 >  SRR4302545.181208/1‑94 (MQ=60)
               AGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCA                                                                                >  SRR4302545.4687710/1‑94 (MQ=60)
               AGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCA                                                                                >  SRR4302545.381525/1‑94 (MQ=60)
                GGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAG                                                                               >  SRR4302545.3270611/1‑94 (MQ=60)
                 GATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGA                                                                              >  SRR4302545.8691207/1‑94 (MQ=60)
                  ATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCGATCATCAGAGGCCTCGCTGGCATGCCCGGCAATGCCCGCAGAC                                                                             >  SRR4302545.1983105/1‑94 (MQ=60)
                  ATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGAC                                                                             <  SRR4302545.1021919/94‑1 (MQ=60)
                  ATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGAC                                                                             >  SRR4302545.6250454/1‑94 (MQ=60)
                  ATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGAC                                                                             >  SRR4302545.1983104/1‑94 (MQ=60)
                   TTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACA                                                                            >  SRR4302545.8537644/1‑94 (MQ=60)
                      TCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTT                                                                         <  SRR4302545.10865428/94‑1 (MQ=60)
                       CCAGCGCATTGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTC                                                                        <  SRR4302545.1003905/94‑1 (MQ=60)
                       CCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTC                                                                        <  SRR4302545.8447557/94‑1 (MQ=60)
                        CAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCC                                                                       >  SRR4302545.5087044/1‑94 (MQ=60)
                         AGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGTCCGGCAATCGCCGCAGACATTTCCG                                                                      >  SRR4302545.5233876/1‑94 (MQ=60)
                            GCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCG                                                                   >  SRR4302545.7921470/1‑94 (MQ=60)
                            GCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCA                                                                   >  SRR4302545.10244826/1‑94 (MQ=60)
                                CGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCAC                                                               <  SRR4302545.4957913/94‑1 (MQ=60)
                                CGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCAC                                                               <  SRR4302545.6275418/94‑1 (MQ=60)
                                 GACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACG                                                              >  SRR4302545.3314326/1‑94 (MQ=60)
                                    GATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCG                                                           >  SRR4302545.2720932/1‑94 (MQ=60)
                                    GATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCG                                                           <  SRR4302545.1309909/94‑1 (MQ=60)
                                     ATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGC                                                          >  SRR4302545.112052/1‑94 (MQ=60)
                                         GGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAAC                                                      >  SRR4302545.7689757/1‑94 (MQ=60)
                                         GGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAAC                                                      >  SRR4302545.3410990/1‑94 (MQ=60)
                                         GGGCAACAGGAATCGCCATCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAAC                                                      <  SRR4302545.4758631/94‑1 (MQ=60)
                                           GCAACACGAATCGCTGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACAC                                                    <  SRR4302545.5943/94‑1 (MQ=60)
                                            GAACAGGAATCGGCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACG                                                   <  SRR4302545.8559246/94‑1 (MQ=60)
                                            CAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACG                                                   <  SRR4302545.8559244/94‑1 (MQ=60)
                                             AACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCCACACGC                                                  >  SRR4302545.8422223/1‑94 (MQ=60)
                                              ACAGGAATCGCTGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCG                                                 <  SRR4302545.1918555/94‑1 (MQ=60)
                                              ACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCG                                                 <  SRR4302545.119389/94‑1 (MQ=60)
                                               CAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGT                                                >  SRR4302545.7523693/1‑94 (MQ=60)
                                                AGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTC                                               <  SRR4302545.5770073/94‑1 (MQ=60)
                                                    ATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGT                                           <  SRR4302545.2480401/94‑1 (MQ=60)
                                                    ATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGT                                           <  SRR4302545.4144151/94‑1 (MQ=60)
                                                    ATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGT                                           <  SRR4302545.1269016/94‑1 (MQ=60)
                                                    ATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGT                                           <  SRR4302545.3223682/94‑1 (MQ=60)
                                                     TCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTT                                          <  SRR4302545.5061134/94‑1 (MQ=60)
                                                       GCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGC                                        <  SRR4302545.982387/94‑1 (MQ=60)
                                                        CCGACATTCCGCCATCATCAGCGGCCTCTCTGGCATGCCCGGCCATCGCCGCAGACATCTCCGGCAGCACGCGCCCAACAGGCGTCGGGTTGGC                                       >  SRR4302545.10336926/1‑94 (MQ=60)
                                                         CGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCA                                      >  SRR4302545.5876745/1‑94 (MQ=60)
                                                          GTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCAC                                     <  SRR4302545.2487468/94‑1 (MQ=60)
                                                          GTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCAC                                     >  SRR4302545.10845843/1‑94 (MQ=60)
                                                           TCATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACC                                    <  SRR4302545.4978090/94‑1 (MQ=60)
                                                             ATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGT                                  >  SRR4302545.1013330/1‑94 (MQ=60)
                                                             ATTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGT                                  <  SRR4302545.9046464/94‑1 (MQ=60)
                                                              TTCCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTT                                 >  SRR4302545.6855889/1‑94 (MQ=60)
                                                                CCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGG                               >  SRR4302545.4585225/1‑94 (MQ=60)
                                                                CCGCCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGG                               >  SRR4302545.4585226/1‑94 (MQ=60)
                                                                  GCCATCATCAGCGGCCTCGCTGGCATGTCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAG                             <  SRR4302545.5326566/94‑1 (MQ=60)
                                                                   CCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGT                            <  SRR4302545.4951507/94‑1 (MQ=60)
                                                                   CCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGT                            <  SRR4302545.7845550/94‑1 (MQ=60)
                                                                   CCATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGT                            <  SRR4302545.5570612/94‑1 (MQ=60)
                                                                    CATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTA                           <  SRR4302545.5893951/94‑1 (MQ=60)
                                                                    CATCATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTA                           <  SRR4302545.5893950/94‑1 (MQ=60)
                                                                      ACATCAGCGGCCTCGGTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAAT                         <  SRR4302545.9428984/94‑1 (MQ=60)
                                                                        ATCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGGCACCGTTGGAGTAATGA                       >  SRR4302545.8738998/1‑94 (MQ=60)
                                                                         TCAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGAT                      <  SRR4302545.7586335/94‑1 (MQ=60)
                                                                          CAGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATA                     >  SRR4302545.576465/1‑94 (MQ=60)
                                                                           CGCGGCCTCGCTGTCATCCCCGGCAATCGCCGCATACATTTCCGGCAGCACCCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAA                    <  SRR4302545.3806168/94‑1 (MQ=60)
                                                                           AGCGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAA                    <  SRR4302545.8636272/94‑1 (MQ=60)
                                                                             CGGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGA                  <  SRR4302545.2217105/94‑1 (MQ=60)
                                                                              GGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAG                 <  SRR4302545.3381902/94‑1 (MQ=60)
                                                                              GGCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAG                 <  SRR4302545.10929787/94‑1 (MQ=60)
                                                                               GCCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGA                <  SRR4302545.8747149/94‑1 (MQ=60)
                                                                                CCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAA               >  SRR4302545.8985903/1‑94 (MQ=60)
                                                                                CCTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGATTGCCACCGCTGGAGCAATGATAAGAGAA               >  SRR4302545.4106902/1‑94 (MQ=60)
                                                                                 CTCGCTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAA              <  SRR4302545.9409996/94‑1 (MQ=60)
                                                                                 CTCGCTGGCATGCCCGGCAATCGCCGCAGACATTGCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAA              <  SRR4302545.8838338/94‑1 (MQ=60)
                                                                                     CTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAA          <  SRR4302545.5228111/94‑1 (MQ=60)
                                                                                     CTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAA          >  SRR4302545.5223052/1‑94 (MQ=60)
                                                                                     CTGGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAA          >  SRR4302545.4680161/1‑94 (MQ=60)
                                                                                       GGCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAA        >  SRR4302545.3965897/1‑94 (MQ=60)
                                                                                        GCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGGGTAATGATAAAAGAAACCAAAAC       >  SRR4302545.616361/1‑94 (MQ=60)
                                                                                        GCATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAAC       >  SRR4302545.4073264/1‑94 (MQ=60)
                                                                                          ATGCCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCG     >  SRR4302545.3290340/1‑94 (MQ=60)
                                                                                             CCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACCCGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCG  >  SRR4302545.3900829/1‑94 (MQ=60)
                                                                                             CCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCG  <  SRR4302545.10818267/94‑1 (MQ=60)
                                                                                             |                                                                                             
CTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGTCCGGCAATCGCCGCAGACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCG  >  NC_000913/1953700‑1953886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑