Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,980,940 +C coding (1/363 nt)
coding (627/627 nt)		 araH ←
araH ←		 ABC‑type arabinose transport system permease component
ABC‑type arabinose transport system permease component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,980,9391.C99.0% 674.4 / ‑1.5 199coding (2/363 nt)araHABC‑type arabinose transport system permease component
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base C (121/76);  total (122/77)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 220 total aligned reads displayed.

GCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGG‑C‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTG  >  NC_000913/1980846‑1981027
                                                                                              |                                                                                         
gCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCgg                                                                                            >  2:10069052/1‑94 (MQ=255)
 ccAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGG‑c                                                                                          <  2:301775/94‑1 (MQ=255)
 ccAGAATAATTCCGGCTATCGCTGATAACAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGG‑c                                                                                          >  1:4048686/1‑94 (MQ=255)
        aatTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCg                                                                                   >  1:4899071/1‑94 (MQ=255)
        aatTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCg                                                                                   >  2:6541880/1‑94 (MQ=255)
         atTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGt                                                                                  >  2:6201733/1‑94 (MQ=255)
          ttCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGtt                                                                                 >  2:8444670/1‑94 (MQ=255)
             cGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTccc                                                                              <  2:7175456/94‑1 (MQ=255)
              cgCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCg                                                                             <  2:2872022/93‑1 (MQ=255)
               gCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGc                                                                            >  2:8165428/1‑94 (MQ=255)
               gCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGc                                                                            >  2:6127894/1‑94 (MQ=255)
                 tATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCa                                                                          >  2:5999484/1‑94 (MQ=255)
                  aTCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACAACCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCaa                                                                         >  2:1173988/1‑94 (MQ=255)
                   tCGCGGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAAt                                                                        <  1:721506/94‑1 (MQ=255)
                    cGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTcc                                                                               <  2:5696525/86‑1 (MQ=255)
                    cGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATc                                                                       >  2:3240437/1‑94 (MQ=255)
                     gCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCg                                                                      <  2:2675723/94‑1 (MQ=255)
                       tGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGcc                                                                    >  2:6682818/1‑94 (MQ=255)
                       tGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGcc                                                                    >  1:1190184/1‑94 (MQ=255)
                        gATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCa                                                                   >  1:7698179/1‑94 (MQ=255)
                          tACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAgg                                                                 <  2:5313313/94‑1 (MQ=255)
                          tACCAGGCCGGAGAGAACAAAGATAATAATTTTGGTCCGAACAACCGGTCCACCCCCCAGACGCGCGGCC‑TCTTCGTCCCCGCCAATCGCCAgg                                                                 <  2:9513630/94‑1 (MQ=255)
                            ccAGTCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGgt                                                               <  2:4004159/94‑1 (MQ=255)
                              aGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGgtgt                                                             >  1:8119492/1‑94 (MQ=255)
                              aGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGgtgt                                                             <  2:1242285/94‑1 (MQ=255)
                               ggCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACCCCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGtt                                                            <  1:7645799/94‑1 (MQ=255)
                               ggCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGtt                                                            >  1:211431/1‑94 (MQ=255)
                                gCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTa                                                           <  1:662718/94‑1 (MQ=255)
                                 ccTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTAc                                                          >  1:3767611/1‑94 (MQ=255)
                                 ccTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTAc                                                          <  1:2356228/94‑1 (MQ=255)
                                 ccTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTAc                                                          <  2:6408590/94‑1 (MQ=255)
                                  cTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACg                                                         <  2:13053/94‑1 (MQ=255)
                                   tGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGa                                                        >  2:1981463/1‑94 (MQ=255)
                                     agagaACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGAcc                                                      >  1:2230486/1‑94 (MQ=255)
                                     agagaACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGAcc                                                      >  2:7793033/1‑94 (MQ=255)
                                     agagaACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGAcc                                                      >  1:4006887/1‑94 (MQ=255)
                                      gagaACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCa                                                     >  2:3620878/1‑94 (MQ=255)
                                      gagaACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCa                                                     <  1:3287454/94‑1 (MQ=255)
                                       agaACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCaa                                                    >  1:6007915/1‑94 (MQ=255)
                                       agaACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCaa                                                    <  1:5523900/94‑1 (MQ=255)
                                        gaACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCAACCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCaaa                                                   >  2:5558732/1‑94 (MQ=255)
                                          aCAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAgg                                                 >  1:7524624/1‑94 (MQ=255)
                                          aCAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAgg                                                 <  2:4875637/94‑1 (MQ=255)
                                          aCAAAGATAATAATTTTGGTGCGAACAACCGGCACCCCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAgg                                                 <  1:5833975/94‑1 (MQ=255)
                                           cAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAggt                                                >  2:348006/1‑94 (MQ=255)
                                            aaaGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAggtg                                               <  2:4484903/94‑1 (MQ=255)
                                            aaaGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAggtg                                               >  2:8346307/1‑94 (MQ=255)
                                               gATAGTAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGtt                                            <  1:7181621/94‑1 (MQ=255)
                                                ataataATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TTCTCTCTCGCGCCTCTCCCCAGGGTGTTGACACCAAAGGTGtttt                                           >  1:4160041/1‑94 (MQ=255)
                                                ataataATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGttt                                           >  1:1010382/1‑94 (MQ=255)
                                                ataataATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCACGGAGTTACGACCAAAGGTGGttt                                           >  1:10556400/1‑94 (MQ=255)
                                                 taataaTTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGtttt                                          <  1:8763877/94‑1 (MQ=255)
                                                 taataaTTGTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGtttt                                          <  1:7410095/94‑1 (MQ=255)
                                                  aataatTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTa                                         >  1:3048556/1‑94 (MQ=255)
                                                  aataatTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTa                                         <  2:7290722/94‑1 (MQ=255)
                                                      atTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTca                                     >  1:10446824/1‑94 (MQ=255)
                                                      atTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTca                                     >  1:941703/1‑94 (MQ=255)
                                                        tttGGTGCTAACAACGGGTACCCCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTcagc                                   <  2:9258664/94‑1 (MQ=255)
                                                        tttGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTcagc                                   >  1:9649837/1‑94 (MQ=255)
                                                         ttGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTcagca                                  <  1:3240437/94‑1 (MQ=255)
                                                            gTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAgcagca                               >  2:9473590/1‑94 (MQ=255)
                                                             tGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCaa                              >  1:7030853/1‑94 (MQ=255)
                                                              gCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGGTTTATTCAGCAGCaaa                             >  1:669242/1‑94 (MQ=255)
                                                               cGAACAACCGGTACCCCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAAc                            <  1:8436125/94‑1 (MQ=255)
                                                               cGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAAc                            <  2:7516612/94‑1 (MQ=255)
                                                                 aacaacGGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCa                          <  2:4256784/94‑1 (MQ=255)
                                                                 aacaacCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCa                          >  1:6260942/1‑94 (MQ=255)
                                                                 aacaacCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCa                          >  1:4387278/1‑94 (MQ=255)
                                                                 aacaacCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCa                          >  1:9231804/1‑94 (MQ=255)
                                                                 aacaacCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCa                          >  2:1401593/1‑94 (MQ=255)
                                                                  acaacCGGTACCCCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCaa                         <  2:10578239/94‑1 (MQ=255)
                                                                    aacCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAg                       >  1:8263872/1‑94 (MQ=255)
                                                                     acCGGTACCCCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGa                      <  1:5114031/94‑1 (MQ=255)
                                                                     acCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGa                      >  1:5069988/1‑94 (MQ=255)
                                                                     acCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGa                      >  1:9712163/1‑94 (MQ=255)
                                                                       cGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCACAGATa                    >  2:9664281/1‑94 (MQ=255)
                                                                       cGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATa                    >  2:7002394/1‑94 (MQ=255)
                                                                        ggTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCACAGGTGGTTTTATTCAGCAGCAAACCAAAGATaa                   >  2:4326347/1‑94 (MQ=255)
                                                                        ggTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATaa                   >  1:287509/1‑94 (MQ=255)
                                                                        ggTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATaa                   <  1:5834525/94‑1 (MQ=255)
                                                                        ggTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATaa                   >  2:1813426/1‑94 (MQ=255)
                                                                           tcacCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAAGCa                <  2:8181038/93‑1 (MQ=255)
                                                                           acacCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCa                <  2:8859446/94‑1 (MQ=255)
                                                                           acacCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCa                <  1:3387922/94‑1 (MQ=255)
                                                                           acacCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCa                >  2:6247562/1‑94 (MQ=255)
                                                                            cacCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAg               >  2:6622959/1‑94 (MQ=255)
                                                                             acCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGa              >  1:7831395/1‑94 (MQ=255)
                                                                             acCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGa              >  1:2251615/1‑94 (MQ=255)
                                                                             acCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGa              >  2:10414768/1‑94 (MQ=255)
                                                                              cccGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGac             >  2:2478031/1‑94 (MQ=255)
                                                                               ccGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGaca            >  1:932913/1‑94 (MQ=255)
                                                                               ccGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGaca            >  1:4729171/1‑94 (MQ=255)
                                                                                cGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGacac           <  2:9218222/94‑1 (MQ=255)
                                                                                 gCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACAcg          >  2:2563088/1‑94 (MQ=255)
                                                                                  ccAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACAcgc         >  1:8872021/1‑94 (MQ=255)
                                                                                  ccAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACAcgc         <  2:2320710/94‑1 (MQ=255)
                                                                                   cAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACAcgcg        <  1:1256869/94‑1 (MQ=255)
                                                                                       cgcgcgGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACgg    <  1:2728897/94‑1 (MQ=255)
                                                                                        gcgcgGCC‑TCTCCGTCCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGt   <  2:174267/94‑1 (MQ=255)
                                                                                         cgcgG‑CTTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTg  <  1:497256/94‑1 (MQ=255)
                                                                                              |                                                                                         
GCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGG‑C‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTG  >  NC_000913/1980846‑1981027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GG‑C‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAG  >  NC_000913/1980847‑1981033
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CCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAAGAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GG‑C                                                                                                >  SRR4302545.4150053/1‑94 (MQ=60)
CCAGAATAATTCCGGCTATCGCTGATAACAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GG‑C                                                                                                >  SRR4302545.4150052/1‑94 (MQ=60)
 CAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GG‑C‑C                                                                                              >  SRR4302545.6800314/1‑94 (MQ=60)
  AGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GG‑Cct                                                                                              <  SRR4302545.1122775/94‑3 (MQ=60)
    AATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCT                                                                                            <  SRR4302545.9953935/94‑1 (MQ=60)
    AATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCT                                                                                            <  SRR4302545.8179693/94‑1 (MQ=60)
    AATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCT                                                                                            <  SRR4302545.2768889/94‑1 (MQ=60)
     ATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTT                                                                                           <  SRR4302545.10014696/94‑1 (MQ=60)
     ATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTT                                                                                           <  SRR4302545.5887487/94‑1 (MQ=60)
     ATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTT                                                                                           <  SRR4302545.5887486/94‑1 (MQ=60)
     ATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTT                                                                                           >  SRR4302545.4184603/1‑94 (MQ=60)
     ATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTT                                                                                           <  SRR4302545.3622231/94‑1 (MQ=60)
      TAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGT‑GGCC‑TCTTC                                                                                          <  SRR4302545.9661658/94‑1 (MQ=60)
      TAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTC                                                                                          <  SRR4302545.354530/94‑1 (MQ=60)
       AATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCG                                                                                         >  SRR4302545.5023451/1‑94 (MQ=60)
        ATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGT                                                                                        <  SRR4302545.8122004/94‑1 (MQ=60)
        ATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGT                                                                                        >  SRR4302545.4028318/1‑94 (MQ=60)
         TTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTT                                                                                       >  SRR4302545.7653420/1‑94 (MQ=60)
              GCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGC                                                                                  >  SRR4302545.9248539/1‑94 (MQ=60)
              GCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGC                                                                                  <  SRR4302545.6819714/94‑1 (MQ=60)
              GCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGC                                                                                  >  SRR4302545.6458908/1‑94 (MQ=60)
               CTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCC                                                                                 >  SRR4302545.5569153/1‑94 (MQ=60)
                TATCGCTGATACCAGGCCTGAGAGAACAAGGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCA                                                                                >  SRR4302545.4551268/1‑94 (MQ=60)
                TATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCA                                                                                >  SRR4302545.4130336/1‑94 (MQ=60)
                 ATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAA                                                                               >  SRR4302545.7009865/1‑94 (MQ=60)
                 ATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAA                                                                               >  SRR4302545.1615431/1‑94 (MQ=60)
                  TCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAAT                                                                              >  SRR4302545.2192901/1‑94 (MQ=60)
                  TCGCGGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAAT                                                                              <  SRR4302545.737731/94‑1 (MQ=60)
                   CGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCagatcaga                                                                             >  SRR4302545.5849047/1‑86 (MQ=60)
                     CTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGC                                                                           >  SRR4302545.3259573/1‑94 (MQ=60)
                      TGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCC                                                                          >  SRR4302545.1216716/1‑94 (MQ=60)
                       GATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCA                                                                         >  SRR4302545.7916120/1‑94 (MQ=60)
                        ATACCAGGCCTGAGAGAACAAAGATAATAATTTGGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAG                                                                        <  SRR4302545.728361/94‑1 (MQ=60)
                         TACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGG                                                                       >  SRR4302545.626960/1‑94 (MQ=60)
                           CCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGT                                                                     <  SRR4302545.235305/94‑1 (MQ=60)
                           CCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGT                                                                     <  SRR4302545.3754801/94‑1 (MQ=60)
                             AGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGT                                                                   >  SRR4302545.8353862/1‑94 (MQ=60)
                              GGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACCCCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTT                                                                  <  SRR4302545.7862163/94‑1 (MQ=60)
                              GGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTT                                                                  >  SRR4302545.215816/1‑94 (MQ=60)
                               GCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTA                                                                 <  SRR4302545.677477/94‑1 (MQ=60)
                                CCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTAC                                                                <  SRR4302545.8564819/94‑1 (MQ=60)
                                CCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTAC                                                                >  SRR4302545.3860609/1‑94 (MQ=60)
                                CCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTAC                                                                <  SRR4302545.2413534/94‑1 (MQ=60)
                                 CTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACG                                                               >  SRR4302545.8384609/1‑94 (MQ=60)
                                 CTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACG                                                               >  SRR4302545.2883913/1‑94 (MQ=60)
                                  TGAGAGAACAAAGATAATAATTTTGATGCGAACACCCGGTACACCCGCCAGCCGCGC‑GGCC‑TCTTCGTTCCCGCCAAGCGCCAGGGTGTTACGA                                                              <  SRR4302545.3704166/94‑1 (MQ=60)
                                    AGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACC                                                            >  SRR4302545.4107103/1‑94 (MQ=60)
                                    AGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACC                                                            >  SRR4302545.2284902/1‑94 (MQ=60)
                                     GAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCA                                                           <  SRR4302545.3367957/94‑1 (MQ=60)
                                     GAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCA                                                           >  SRR4302545.2302384/1‑94 (MQ=60)
                                      AGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAA                                                          >  SRR4302545.6170110/1‑94 (MQ=60)
                                      AGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAA                                                          <  SRR4302545.5669679/94‑1 (MQ=60)
                                         ACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTGCCGCCAATCGCCAGGGTGTTACGACCAAAGG                                                       >  SRR4302545.9778476/1‑94 (MQ=60)
                                         ACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGG                                                       >  SRR4302545.7737503/1‑94 (MQ=60)
                                         ACAAAGATAATAATTTTGGTGCGAACAACCGGCACCCCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGG                                                       <  SRR4302545.5991028/94‑1 (MQ=60)
                                          CAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCAGC‑C‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGG                                                      >  SRR4302545.786245/1‑94 (MQ=60)
                                          CAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGT                                                      >  SRR4302545.1237212/1‑94 (MQ=60)
                                           AAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTG                                                     >  SRR4302545.2879510/1‑94 (MQ=60)
                                            AAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGG                                                    >  SRR4302545.2007430/1‑94 (MQ=60)
                                            AAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGG                                                    <  SRR4302545.5572745/94‑1 (MQ=60)
                                            AAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGG                                                    <  SRR4302545.6155159/94‑1 (MQ=60)
                                              GATAGTAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTT                                                  <  SRR4302545.7381285/94‑1 (MQ=60)
                                               ATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTT                                                 >  SRR4302545.10867324/1‑94 (MQ=60)
                                               ATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTT                                                 >  SRR4302545.1032711/1‑94 (MQ=60)
                                               ATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCACGGAGTTACGACCAAAGGTGGTTT                                                 >  SRR4302545.10867331/1‑94 (MQ=60)
                                               ATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GG‑Ccttctctctcgcgcctctccccagggtgttgacaccaaaggtgtttt                                                 >  SRR4302545.4263891/1‑47 (MQ=60)
                                                TAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTT                                                <  SRR4302545.7619476/94‑1 (MQ=60)
                                                TAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTT                                                <  SRR4302545.9018247/94‑1 (MQ=60)
                                                TAATAATTGTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTT                                                <  SRR4302545.7619477/94‑1 (MQ=60)
                                                 AATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTA                                               >  SRR4302545.9326412/1‑94 (MQ=60)
                                                 AATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTA                                               >  SRR4302545.3123293/1‑94 (MQ=60)
                                                     ATTTTGGTGCGAACAACCGGTACCCCCGCCAGACGCGC‑GGCC‑TCTTCGTCCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCA                                           <  SRR4302545.6919722/94‑1 (MQ=60)
                                                     ATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTTCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCA                                           >  SRR4302545.10754695/1‑94 (MQ=60)
                                                     ATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCA                                           >  SRR4302545.10754696/1‑94 (MQ=60)
                                                     ATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCA                                           >  SRR4302545.962822/1‑94 (MQ=60)
                                                     ATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCA                                           >  SRR4302545.7858158/1‑94 (MQ=60)
                                                       TTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGC                                         >  SRR4302545.9934999/1‑94 (MQ=60)
                                                        TTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCA                                        >  SRR4302545.7541217/1‑94 (MQ=60)
                                                        TTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCA                                        <  SRR4302545.3320014/94‑1 (MQ=60)
                                                          GGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGC                                      >  SRR4302545.9577116/1‑94 (MQ=60)
                                                           gcgcaaacacccggtgccccccCCAGACGCGC‑GGCC‑TCTTCGTCCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCA                                     <  SRR4302545.131520/72‑1 (MQ=60)
                                                            TGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAA                                    >  SRR4302545.7224970/1‑94 (MQ=60)
                                                             GCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAA                                   >  SRR4302545.3418702/1‑94 (MQ=60)
                                                             GCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGGTTTATTCAGCAGCAAA                                   >  SRR4302545.684150/1‑94 (MQ=60)
                                                              CGAACAACCGGTACCCCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAAC                                  <  SRR4302545.8680052/94‑1 (MQ=60)
                                                                AACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCA                                >  SRR4302545.9502474/1‑94 (MQ=60)
                                                                AACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCA                                >  SRR4302545.6431627/1‑94 (MQ=60)
                                                                AACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCA                                >  SRR4302545.4498406/1‑94 (MQ=60)
                                                                 ACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAA                               >  SRR4302545.4872049/1‑94 (MQ=60)
                                                                   aacgggtgctCCCGCCAGACGCGC‑GGCC‑TCGCCGTTCCCGCCAATCGCCAGGGGGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAG                             <  SRR4302545.5358264/84‑1 (MQ=60)
                                                                   AACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAG                             >  SRR4302545.8503055/1‑94 (MQ=60)
                                                                    ACCGGTACCCCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGA                            <  SRR4302545.5245009/94‑1 (MQ=60)
                                                                    ACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGA                            >  SRR4302545.10001391/1‑94 (MQ=60)
                                                                    ACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGA                            <  SRR4302545.5245008/94‑1 (MQ=60)
                                                                    ACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGA                            >  SRR4302545.5199132/1‑94 (MQ=60)
                                                                      CGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATA                          >  SRR4302545.5195253/1‑94 (MQ=60)
                                                                       GGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAA                         >  SRR4302545.293532/1‑94 (MQ=60)
                                                                       GGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAA                         <  SRR4302545.5991589/94‑1 (MQ=60)
                                                                       GGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAA                         >  SRR4302545.4638456/1‑94 (MQ=60)
                                                                          ACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCA                      <  SRR4302545.3071753/94‑1 (MQ=60)
                                                                          ACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCA                      <  SRR4302545.3470480/94‑1 (MQ=60)
                                                                            ACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGA                    >  SRR4302545.2306451/1‑94 (MQ=60)
                                                                            ACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGA                    >  SRR4302545.8053340/1‑94 (MQ=60)
                                                                            ACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGA                    >  SRR4302545.3359569/1‑94 (MQ=60)
                                                                              CCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACA                  >  SRR4302545.8963517/1‑94 (MQ=60)
                                                                              CCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACA                  >  SRR4302545.953832/1‑94 (MQ=60)
                                                                              CCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACA                  >  SRR4302545.4849779/1‑94 (MQ=60)
                                                                                 CCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGC               >  SRR4302545.9129762/1‑94 (MQ=60)
                                                                                  CAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCATCAGCAAACCAAAGATAATCAGACACGCG              <  SRR4302545.9088690/94‑1 (MQ=60)
                                                                                  CAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCGAACCAAAGATAATCAGACACGCG              >  SRR4302545.5991408/1‑94 (MQ=60)
                                                                                  CAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCG              <  SRR4302545.1285033/94‑1 (MQ=60)
                                                                                  CAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCG              >  SRR4302545.5043691/1‑94 (MQ=60)
                                                                                  CAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACAAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCG              <  SRR4302545.790891/94‑1 (MQ=60)
                                                                                   AGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGA             >  SRR4302545.4379173/1‑94 (MQ=60)
                                                                                    GACGCGC‑GGCC‑TCTTCGTCCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGAC            <  SRR4302545.10142541/94‑1 (MQ=60)
                                                                                      CGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGG          <  SRR4302545.2794718/94‑1 (MQ=60)
                                                                                        CGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGGTTTATTCAGCAGAAAACCAAAGATAATCAGACACGCGACGGTG        >  SRR4302545.7393791/1‑94 (MQ=60)
                                                                                        CGC‑GG‑CTTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTG        <  SRR4302545.508387/94‑1 (MQ=60)
                                                                                         GC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGGTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGA       >  SRR4302545.6561696/1‑94 (MQ=60)
                                                                                          C‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAG      >  SRR4302545.8661870/1‑94 (MQ=60)
                                                                                            ggcC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGC     >  SRR4302545.532458/4‑94 (MQ=60)
                                                                                             C‑C‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCA   >  SRR4302545.6926466/1‑94 (MQ=60)
                                                                                               C‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAG  >  SRR4302545.3828909/1‑94 (MQ=60)
                                                                                               C‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAG  >  SRR4302545.2803621/1‑94 (MQ=60)
                                                                                               C‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAG  <  SRR4302545.4682954/94‑1 (MQ=60)
                                                                                               C‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAG  >  SRR4302545.1761840/1‑94 (MQ=60)
                                                                                               |                                                                                              
CCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GG‑C‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAG  >  NC_000913/1980847‑1981033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑