Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,996,614 T→C D297G (GAC→GGC)  b1919 ← putative 1‑aminocyclopropane‑1‑carboxylate deaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,996,6140TC98.9% 622.5 / ‑2.3 186D297G (GAC→GGC) b1919putative 1‑aminocyclopropane‑1‑carboxylate deaminase
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (105/79);  total (106/80)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01

Only 100 of 192 total aligned reads displayed.

GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTCCATGCCTT  >  NC_000913/1996525‑1996704
                                                                                         |                                                                                          
ggggATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAAt                                                                                        <  2:9192093/94‑1 (MQ=255)
ggggATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAAt                                                                                        <  2:10378650/94‑1 (MQ=255)
ggggATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAAt                                                                                        <  1:4626305/94‑1 (MQ=255)
 gggATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATc                                                                                       >  2:5387646/1‑94 (MQ=255)
 gggATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATc                                                                                       <  1:9486134/94‑1 (MQ=255)
  ggATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATcg                                                                                      >  1:2798886/1‑93 (MQ=255)
  ggATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCa                                                                                      >  1:2253521/1‑94 (MQ=255)
  ggATGATAGGCAAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCa                                                                                      <  2:7838127/94‑1 (MQ=255)
    atgatAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGc                                                                                    >  1:4145841/1‑94 (MQ=255)
     tgatAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGcc                                                                                   >  2:5076301/1‑94 (MQ=255)
        tAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCACCg                                                                                >  2:7536163/1‑94 (MQ=255)
           gCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCa                                                                             >  1:4391405/1‑94 (MQ=255)
            cGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCAt                                                                            >  1:6564901/1‑94 (MQ=255)
             gAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATc                                                                           >  1:8667457/1‑94 (MQ=255)
             gAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATc                                                                           >  2:4018706/1‑94 (MQ=255)
               aCAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGc                                                                         >  2:4929095/1‑94 (MQ=255)
               aCAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGc                                                                         <  2:2769215/94‑1 (MQ=255)
                cAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCt                                                                        <  1:5162384/94‑1 (MQ=255)
                 aGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACGGCCAAGCAGCCCCGCCCTCGcat                                                                       >  1:8881233/1‑92 (MQ=255)
                  gcgcAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCttt                                                                      <  2:10533873/94‑1 (MQ=255)
                   cgcAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCtttt                                                                     <  2:9314498/94‑1 (MQ=255)
                      aGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCg                                                                  >  1:1494657/1‑94 (MQ=255)
                      aGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCg                                                                  >  1:8687778/1‑94 (MQ=255)
                      aGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCg                                                                  >  2:7297462/1‑94 (MQ=255)
                       ggCGCACCGCCGGTATGAATAAACAGAATCGGGCCTTCATCTTGGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCgg                                                                 <  2:534380/94‑1 (MQ=255)
                         cgcACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGta                                                               >  1:8131868/1‑94 (MQ=255)
                         cactccgccgGTATGAAAAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGta                                                               <  1:8850364/90‑1 (MQ=255)
                          gcACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGtat                                                              <  1:9657258/94‑1 (MQ=255)
                           cACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGtata                                                             >  1:2514499/1‑94 (MQ=255)
                            aCCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATac                                                            >  1:2856350/1‑94 (MQ=255)
                             ccgcctGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATaca                                                           <  2:1581698/94‑1 (MQ=255)
                             ccgccgGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATaca                                                           >  2:6434810/1‑94 (MQ=255)
                              cgccgGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATacac                                                          <  2:8925148/94‑1 (MQ=255)
                               gccgGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATAcaca                                                         <  1:4905848/94‑1 (MQ=255)
                               gccgGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATAcaca                                                         <  2:6419586/94‑1 (MQ=255)
                                ccgGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAg                                                        <  2:2137642/94‑1 (MQ=255)
                                 cgGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATATCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAgg                                                       >  1:4338137/1‑94 (MQ=255)
                                  ggTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGa                                                      <  2:7657432/94‑1 (MQ=255)
                                   gTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGAt                                                     <  1:9161975/94‑1 (MQ=255)
                                    tATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATc                                                    >  1:8228974/1‑94 (MQ=255)
                                     aTGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCa                                                   <  2:9393494/94‑1 (MQ=255)
                                       gAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAg                                                 >  1:9672856/1‑94 (MQ=255)
                                        aaTAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGc                                                >  2:2938705/1‑94 (MQ=255)
                                         aTAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATGCCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCa                                               <  2:2571696/94‑1 (MQ=255)
                                         aTAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCa                                               <  1:7870091/94‑1 (MQ=255)
                                          tAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAg                                              >  2:4256528/1‑94 (MQ=255)
                                          tAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAg                                              >  1:9360515/1‑94 (MQ=255)
                                           aaaCAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGa                                             >  1:9951201/1‑94 (MQ=255)
                                           aaaCAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGa                                             >  2:4783305/1‑94 (MQ=255)
                                            aaCAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGaa                                            <  2:10268519/94‑1 (MQ=255)
                                             aCAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAAt                                           <  2:3120623/94‑1 (MQ=255)
                                             aCAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAAt                                           <  2:3447870/94‑1 (MQ=255)
                                              cAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATg                                          >  1:7531276/1‑94 (MQ=255)
                                              cAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATg                                          <  2:8029468/94‑1 (MQ=255)
                                               aGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGc                                         <  2:3571614/94‑1 (MQ=255)
                                                gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGcc                                        >  2:1962277/1‑94 (MQ=255)
                                                  aTCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCtt                                      >  1:8499456/1‑94 (MQ=255)
                                                  aTCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCtt                                      <  1:3399274/94‑1 (MQ=255)
                                                   tCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTc                                     <  2:9839155/94‑1 (MQ=255)
                                                   tCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTc                                     <  1:3783742/94‑1 (MQ=255)
                                                    cGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTcc                                    <  2:2196404/94‑1 (MQ=255)
                                                     ggCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCa                                   >  2:6196319/1‑94 (MQ=255)
                                                     ggCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCa                                   >  1:8182118/1‑94 (MQ=255)
                                                       cccTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGc                                 <  2:5881350/94‑1 (MQ=255)
                                                        ccTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGcc                                >  2:5648277/1‑94 (MQ=255)
                                                        ccTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGcc                                >  1:10278979/1‑94 (MQ=255)
                                                        ccTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGcc                                <  1:8041965/94‑1 (MQ=255)
                                                        ccTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGcc                                >  1:9446552/1‑94 (MQ=255)
                                                         cTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCcg                               >  2:3347291/1‑94 (MQ=255)
                                                          ttCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCcgc                              >  2:2739723/1‑94 (MQ=255)
                                                           tCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCcgcg                             <  2:4589267/94‑1 (MQ=255)
                                                           tCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCcgcg                             >  1:6055585/1‑94 (MQ=255)
                                                            cATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCgcgc                            >  1:7851497/1‑94 (MQ=255)
                                                              tCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCa                          <  1:10267919/94‑1 (MQ=255)
                                                               cTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAg                         >  1:5407456/1‑94 (MQ=255)
                                                               cTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAg                         >  2:8486742/1‑94 (MQ=255)
                                                               cTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAg                         >  1:7230169/1‑94 (MQ=255)
                                                                tttGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGc                        >  1:822340/1‑94 (MQ=255)
                                                                 ttGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCa                       <  2:9240733/94‑1 (MQ=255)
                                                                  tGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCaa                      >  1:1197430/1‑94 (MQ=255)
                                                                  tGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCaa                      >  1:3023527/1‑94 (MQ=255)
                                                                   gAACCGTTTCTGACTTATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAAt                     <  1:2055363/94‑1 (MQ=255)
                                                                     aGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAAttt                   >  2:5838355/1‑94 (MQ=255)
                                                                     aGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAAttt                   >  1:8323250/1‑94 (MQ=255)
                                                                     aGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAAttt                   <  1:1921749/94‑1 (MQ=255)
                                                                       cGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCa                 <  2:10043227/94‑1 (MQ=255)
                                                                       cGTTTCTGACTGAGACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCa                 <  1:6024626/94‑1 (MQ=255)
                                                                        gTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCAc                >  1:54385/1‑94 (MQ=255)
                                                                        gTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCAc                >  2:9044340/1‑94 (MQ=255)
                                                                          ttCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTg              >  2:5454621/1‑94 (MQ=255)
                                                                            ctgacaaaTCCCGCCAACCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCt            <  1:3758210/94‑1 (MQ=255)
                                                                             tgactgaTACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCtt           >  2:3077442/1‑94 (MQ=255)
                                                                             tgactgaTACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCtt           >  1:6756733/1‑94 (MQ=255)
                                                                               actgaTACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTcc         <  2:8190221/94‑1 (MQ=255)
                                                                               actgaTACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTcc         >  1:3770415/1‑94 (MQ=255)
                                                                               actgaTACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTcc         >  1:7270879/1‑94 (MQ=255)
                                                                               actgaTACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTcc         >  1:9519417/1‑94 (MQ=255)
                                                                                 tgaTACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTCCAt       <  2:8352947/94‑1 (MQ=255)
                                                                                  gaTACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTCCATg      <  1:4040650/94‑1 (MQ=255)
                                                                                   aTACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTCCATGc     <  1:4211143/94‑1 (MQ=255)
                                                                                         |                                                                                          
GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTCCATGCCTT  >  NC_000913/1996525‑1996704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTCCATGCCTTCGT  >  NC_000913/1996522‑1996707
                                                                                            |                                                                                             
CGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGATTGATACCGCC                                                                                              >  SRR4302545.1898247/1‑94 (MQ=60)
   GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAAT                                                                                           <  SRR4302545.4744587/94‑1 (MQ=60)
    GGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATC                                                                                          <  SRR4302545.9621014/94‑1 (MQ=60)
    GGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATC                                                                                          <  SRR4302545.9765288/94‑1 (MQ=60)
     GGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCG                                                                                         >  SRR4302545.2866576/1‑94 (MQ=60)
     GGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCA                                                                                         >  SRR4302545.2308402/1‑94 (MQ=60)
       ATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGC                                                                                       >  SRR4302545.4249410/1‑94 (MQ=60)
          ATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCC                                                                                    <  SRR4302545.7754504/94‑1 (MQ=60)
              GCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCA                                                                                >  SRR4302545.4502671/1‑94 (MQ=60)
               CGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCAT                                                                               >  SRR4302545.6744525/1‑94 (MQ=60)
                GAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATC                                                                              >  SRR4302545.8918884/1‑94 (MQ=60)
                   CAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCT                                                                           <  SRR4302545.5295146/94‑1 (MQ=60)
                    AGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACGGCCAAGCAGCCCCGCCCTCGCAT                                                                          >  SRR4302545.9139307/1‑94 (MQ=60)
                    AGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTT                                                                          >  SRR4302545.4293722/1‑94 (MQ=60)
                      ccccagcgcccccgctgtaaaaaaAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTT                                                                        <  SRR4302545.5944793/70‑1 (MQ=60)
                        CAGGCGCACCGCCGGTATGAATAAACAGAATCGTCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCC                                                                      <  SRR4302545.5177336/94‑1 (MQ=60)
                         AGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCG                                                                     >  SRR4302545.1529213/1‑94 (MQ=60)
                         AGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCG                                                                     >  SRR4302545.1529214/1‑94 (MQ=60)
                         AGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCG                                                                     >  SRR4302545.8939794/1‑94 (MQ=60)
                          GGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGG                                                                    >  SRR4302545.6998620/1‑94 (MQ=60)
                            cactCCGCCGGTATGAAAAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTA                                                                  <  SRR4302545.9107432/90‑1 (MQ=60)
                            CGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTA                                                                  >  SRR4302545.8366737/1‑94 (MQ=60)
                            CGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCCGTA                                                                  >  SRR4302545.457384/1‑94 (MQ=60)
                             GCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTAT                                                                 <  SRR4302545.9943148/94‑1 (MQ=60)
                              CACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATA                                                                >  SRR4302545.2575277/1‑94 (MQ=60)
                              CACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATA                                                                <  SRR4302545.9117914/94‑1 (MQ=60)
                              CACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATA                                                                >  SRR4302545.4946803/1‑94 (MQ=60)
                               ACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATAC                                                               >  SRR4302545.2925400/1‑94 (MQ=60)
                                  GCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACA                                                            <  SRR4302545.5030363/94‑1 (MQ=60)
                                   CCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAG                                                           >  SRR4302545.4333146/1‑94 (MQ=60)
                                    CGGTATGATTATACAGAATCGGCCCTTCATCTTTGCAGCATATCTGACTGATCCCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGG                                                          <  SRR4302545.9679047/94‑1 (MQ=60)
                                    CGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATATCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGG                                                          >  SRR4302545.4447596/1‑94 (MQ=60)
                                     GGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGA                                                         <  SRR4302545.10173821/94‑1 (MQ=60)
                                     GGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGA                                                         <  SRR4302545.3875329/94‑1 (MQ=60)
                                      GTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGAT                                                        <  SRR4302545.9430875/94‑1 (MQ=60)
                                      GTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGAT                                                        <  SRR4302545.9430876/94‑1 (MQ=60)
                                       TATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATC                                                       >  SRR4302545.554698/1‑94 (MQ=60)
                                       TATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATC                                                       >  SRR4302545.8467265/1‑94 (MQ=60)
                                       TATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATC                                                       <  SRR4302545.969216/94‑1 (MQ=60)
                                        ATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCA                                                      >  SRR4302545.4500261/1‑94 (MQ=60)
                                         TGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAA                                                     >  SRR4302545.8444979/1‑94 (MQ=60)
                                          GAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAG                                                    >  SRR4302545.9959785/1‑94 (MQ=60)
                                            ATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCA                                                  <  SRR4302545.8093372/94‑1 (MQ=60)
                                            ATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCA                                                  <  SRR4302545.8093373/94‑1 (MQ=60)
                                             TAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAG                                                 >  SRR4302545.9635091/1‑94 (MQ=60)
                                              AAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGA                                                >  SRR4302545.10246530/1‑94 (MQ=60)
                                              AAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGA                                                >  SRR4302545.10246532/1‑94 (MQ=60)
                                                 CAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATG                                             >  SRR4302545.7744326/1‑94 (MQ=60)
                                                  AGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGC                                            >  SRR4302545.8170411/1‑94 (MQ=60)
                                                     ATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTT                                         <  SRR4302545.3482147/94‑1 (MQ=60)
                                                     ATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTT                                         <  SRR4302545.764645/94‑1 (MQ=60)
                                                     ATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTT                                         >  SRR4302545.8745077/1‑94 (MQ=60)
                                                     ATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTT                                         <  SRR4302545.8800674/94‑1 (MQ=60)
                                                     ATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTT                                         <  SRR4302545.9050507/94‑1 (MQ=60)
                                                      TCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTC                                        <  SRR4302545.3877402/94‑1 (MQ=60)
                                                       CGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCC                                       >  SRR4302545.6851634/1‑94 (MQ=60)
                                                        GGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCA                                      >  SRR4302545.8418836/1‑94 (MQ=60)
                                                        GGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCA                                      >  SRR4302545.8418835/1‑94 (MQ=60)
                                                          CCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGC                                    >  SRR4302545.2142494/1‑94 (MQ=60)
                                                          CCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGC                                    >  SRR4302545.7433310/1‑94 (MQ=60)
                                                           CCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCC                                   <  SRR4302545.1075551/94‑1 (MQ=60)
                                                           CCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCC                                   >  SRR4302545.9724221/1‑94 (MQ=60)
                                                           CCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCC                                   <  SRR4302545.8271406/94‑1 (MQ=60)
                                                           CCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCC                                   >  SRR4302545.10582160/1‑94 (MQ=60)
                                                              TCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCG                                >  SRR4302545.6219782/1‑94 (MQ=60)
                                                              TCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCG                                <  SRR4302545.2313298/94‑1 (MQ=60)
                                                               CATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGC                               >  SRR4302545.8074144/1‑94 (MQ=60)
                                                                ATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCC                              >  SRR4302545.6427719/1‑94 (MQ=60)
                                                                 TCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCA                             <  SRR4302545.10570849/94‑1 (MQ=60)
                                                                  CTTTGAAGCTTTTCTGACTGATGCCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAG                            <  SRR4302545.4037492/94‑1 (MQ=60)
                                                                  CTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAG                            >  SRR4302545.2915570/1‑94 (MQ=60)
                                                                  CTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAG                            >  SRR4302545.7432202/1‑94 (MQ=60)
                                                                  CTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAG                            >  SRR4302545.5548525/1‑94 (MQ=60)
                                                                   TTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGC                           >  SRR4302545.840779/1‑94 (MQ=60)
                                                                    TTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCA                          >  SRR4302545.2668633/1‑94 (MQ=60)
                                                                     TGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAA                         >  SRR4302545.1224287/1‑94 (MQ=60)
                                                                     TGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAA                         >  SRR4302545.8491712/1‑94 (MQ=60)
                                                                     TGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAA                         >  SRR4302545.3097486/1‑94 (MQ=60)
                                                                      GAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAAT                        >  SRR4302545.9813959/1‑94 (MQ=60)
                                                                      GAACCGTTTCTGACTTATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAAT                        <  SRR4302545.2106000/94‑1 (MQ=60)
                                                                       AAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATT                       <  SRR4302545.9787855/94‑1 (MQ=60)
                                                                       AAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATT                       >  SRR4302545.3493419/1‑94 (MQ=60)
                                                                        AGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTT                      >  SRR4302545.4181013/1‑94 (MQ=60)
                                                                        AGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTT                      <  SRR4302545.1969256/94‑1 (MQ=60)
                                                                        AGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTT                      >  SRR4302545.8564442/1‑94 (MQ=60)
                                                                        AGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTT                      <  SRR4302545.8649762/94‑1 (MQ=60)
                                                                          CGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCA                    >  SRR4302545.8937970/1‑94 (MQ=60)
                                                                          CGTTTCTGACTGAGACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCA                    <  SRR4302545.6187351/94‑1 (MQ=60)
                                                                           GTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCAC                   >  SRR4302545.55617/1‑94 (MQ=60)
                                                                           GTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCAC                   >  SRR4302545.5628544/1‑94 (MQ=60)
                                                                             TTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTG                 >  SRR4302545.2037628/1‑94 (MQ=60)
                                                                                TGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTT              >  SRR4302545.6942001/1‑94 (MQ=60)
                                                                                TGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTT              >  SRR4302545.3354405/1‑94 (MQ=60)
                                                                                  ACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTCC            >  SRR4302545.3863482/1‑94 (MQ=60)
                                                                                  ACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTCC            >  SRR4302545.9799710/1‑94 (MQ=60)
                                                                                  ACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTCC            >  SRR4302545.7474724/1‑94 (MQ=60)
                                                                                   CTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTCCA           >  SRR4302545.2980983/1‑94 (MQ=60)
                                                                                     GATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTCCATG         <  SRR4302545.4141817/94‑1 (MQ=60)
                                                                                      ATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTCCATGC        <  SRR4302545.4316312/94‑1 (MQ=60)
                                                                                       TACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTCCATGCC       >  SRR4302545.551587/1‑94 (MQ=60)
                                                                                         CCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTCCATGCCTT     <  SRR4302545.1930615/94‑1 (MQ=60)
                                                                                          CGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTCCATGCCTTC    <  SRR4302545.1666833/94‑1 (MQ=60)
                                                                                           GCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTCCATGCCTTCG   >  SRR4302545.7966784/1‑94 (MQ=60)
                                                                                           GCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTCCATGCCTTCG   <  SRR4302545.686120/94‑1 (MQ=60)
                                                                                            CCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTCCATGCCTTCGT  <  SRR4302545.1441901/94‑1 (MQ=60)
                                                                                            CCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTCCATGCCTTCGT  <  SRR4302545.2350333/94‑1 (MQ=60)
                                                                                            |                                                                                             
CGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTCCATGCCTTCGT  >  NC_000913/1996522‑1996707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑