Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,996,614 T→C D297G (GAC→GGC)  b1919 ← putative 1‑aminocyclopropane‑1‑carboxylate deaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,996,6140TC100.0% 667.9 / NA 194D297G (GAC→GGC) b1919putative 1‑aminocyclopropane‑1‑carboxylate deaminase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (92/102);  total (92/102)

Only 100 of 199 total aligned reads displayed.

GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTCCATGCCT  >  NC_000913/1996525‑1996703
                                                                                         |                                                                                         
ggggATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCaa                                                                                        <  2:4888637/93‑1 (MQ=255)
ggggATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCaa                                                                                        >  1:5913410/1‑93 (MQ=255)
ggggATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCaa                                                                                        <  1:4010874/93‑1 (MQ=255)
 gggATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAAt                                                                                       >  2:446776/1‑93 (MQ=255)
 gggATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAAt                                                                                       >  1:2056803/1‑93 (MQ=255)
  ggATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATc                                                                                      >  2:5179301/1‑93 (MQ=255)
   gatgatAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCa                                                                                     >  1:6212967/1‑93 (MQ=255)
   gatgatAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCa                                                                                     >  1:2670062/1‑93 (MQ=255)
     tgatAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGc                                                                                   >  2:5004007/1‑93 (MQ=255)
     tgatAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGc                                                                                   >  2:6170857/1‑93 (MQ=255)
      gatAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAGTCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGcc                                                                                  <  1:3781376/93‑1 (MQ=255)
      gatAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGcc                                                                                  >  1:1020514/1‑93 (MQ=255)
      gatAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGcc                                                                                  <  2:5710102/93‑1 (MQ=255)
      gatAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGcc                                                                                  <  2:700681/93‑1 (MQ=255)
       atAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGccc                                                                                 <  2:4094124/93‑1 (MQ=255)
       atAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGccc                                                                                 <  1:5702039/93‑1 (MQ=255)
        tAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGcccc                                                                                <  2:5673744/93‑1 (MQ=255)
        tAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGcccc                                                                                <  2:480367/93‑1 (MQ=255)
          ggCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGc                                                                              >  2:3289269/1‑93 (MQ=255)
          ggCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGc                                                                              >  1:238868/1‑93 (MQ=255)
             gAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCAt                                                                           >  1:3606656/1‑93 (MQ=255)
              aaCAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATc                                                                          <  2:6457994/93‑1 (MQ=255)
              aaCAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATc                                                                          <  2:2571435/93‑1 (MQ=255)
              aaCAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATc                                                                          >  2:602800/1‑93 (MQ=255)
              aaCAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATc                                                                          <  1:171939/93‑1 (MQ=255)
               aCAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCg                                                                         >  1:833786/1‑93 (MQ=255)
                  gcgcAGGCGCGCCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATGCCGCCAATCAGCCCCGCCATCGCtt                                                                      <  2:2090840/93‑1 (MQ=255)
                  gcgcAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCtt                                                                      >  2:2042397/1‑93 (MQ=255)
                    gcAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCtttt                                                                    >  2:4667520/1‑93 (MQ=255)
                    gcAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCtttt                                                                    >  1:3076946/1‑93 (MQ=255)
                    gcAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCtttt                                                                    <  2:4392409/93‑1 (MQ=255)
                      aGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTcc                                                                  >  2:2205504/1‑93 (MQ=255)
                      aGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTcc                                                                  >  2:3634326/1‑93 (MQ=255)
                      aGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTcc                                                                  >  2:1804495/1‑93 (MQ=255)
                       ggCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCg                                                                 >  2:5937646/1‑93 (MQ=255)
                        gcgcACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCgg                                                                <  1:6286351/93‑1 (MQ=255)
                         cgcACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGt                                                               >  1:1336306/1‑93 (MQ=255)
                          gcACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGta                                                              <  2:1450368/93‑1 (MQ=255)
                          gcACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGta                                                              <  2:4609976/93‑1 (MQ=255)
                            aCCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGtata                                                            >  1:4338855/1‑93 (MQ=255)
                            aCCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGACCCGCCATCGCTTTTCCGGtata                                                            >  2:1517105/1‑93 (MQ=255)
                             ccgccgGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATac                                                           <  2:3036845/93‑1 (MQ=255)
                              cgccgGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATaca                                                          <  2:413364/93‑1 (MQ=255)
                              cgccgGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATaca                                                          <  2:4555949/93‑1 (MQ=255)
                               gccgGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATacac                                                         >  2:3758168/1‑93 (MQ=255)
                               cccgGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATacac                                                         <  2:6031914/92‑1 (MQ=255)
                                ccgGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATAcaca                                                        >  2:2405466/1‑93 (MQ=255)
                                ccgGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATAcaca                                                        >  1:4462899/1‑93 (MQ=255)
                                 cgGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAg                                                       <  2:268725/93‑1 (MQ=255)
                                 cgGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAg                                                       <  1:5055578/93‑1 (MQ=255)
                                 cgGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAg                                                       >  2:5383774/1‑93 (MQ=255)
                                  ggTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAgg                                                      >  1:2818644/1‑93 (MQ=255)
                                   gTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGa                                                     <  1:1784930/93‑1 (MQ=255)
                                    tATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGAt                                                    >  2:4044632/1‑93 (MQ=255)
                                      tGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCa                                                  <  2:1130187/93‑1 (MQ=255)
                                      tGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCa                                                  >  2:3339171/1‑93 (MQ=255)
                                       gAATAAACAGAATCGTCCCTTCATCTTTGAAGCGTTGCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCaa                                                 <  1:998334/93‑1 (MQ=255)
                                       gAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCaa                                                 <  1:286002/93‑1 (MQ=255)
                                       gAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCaa                                                 <  1:4615669/93‑1 (MQ=255)
                                       gAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACGGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCaa                                                 <  1:3395378/93‑1 (MQ=255)
                                        aaTAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAg                                                >  2:1255160/1‑93 (MQ=255)
                                        aaTAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAg                                                <  1:3472519/93‑1 (MQ=255)
                                         aTAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGc                                               <  1:2524540/93‑1 (MQ=255)
                                            aaCAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGa                                            >  2:5016306/1‑93 (MQ=255)
                                            aaCAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGa                                            >  1:199935/1‑93 (MQ=255)
                                             aCAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGaa                                           >  1:2994900/1‑93 (MQ=255)
                                             aCAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGaa                                           <  1:5434597/93‑1 (MQ=255)
                                             aCAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGaa                                           <  1:4804647/93‑1 (MQ=255)
                                             aCAGAATCGGCCCTTCATCTGTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGaa                                           >  2:6220969/1‑93 (MQ=255)
                                              cAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAAt                                          <  1:1593700/93‑1 (MQ=255)
                                                gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGc                                        >  2:3843807/1‑93 (MQ=255)
                                                gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGc                                        >  1:1454492/1‑93 (MQ=255)
                                                  aTCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCt                                      <  2:4945804/93‑1 (MQ=255)
                                                    ctgCCCTTCATCTTTCAAGCGTTTCTGACTGATACCGCCAATCACCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTc                                    <  1:562569/91‑1 (MQ=255)
                                                     tgCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTcc                                   <  2:3856612/92‑1 (MQ=255)
                                                        ccTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGc                                >  1:4856372/1‑93 (MQ=255)
                                                        ccTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGc                                >  2:5195271/1‑93 (MQ=255)
                                                        ccTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGc                                >  1:4043262/1‑93 (MQ=255)
                                                          ttCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCcg                              >  2:335838/1‑93 (MQ=255)
                                                           tCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCcgc                             <  1:3551632/93‑1 (MQ=255)
                                                             aTCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCgcgc                           <  2:2800121/93‑1 (MQ=255)
                                                              tCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGcc                          >  1:5553825/1‑93 (MQ=255)
                                                              tCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGcc                          >  1:4686434/1‑93 (MQ=255)
                                                               cTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCa                         <  2:5575314/93‑1 (MQ=255)
                                                                 ttGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGc                       <  1:5197752/93‑1 (MQ=255)
                                                                    aaGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAAt                    >  1:3816428/1‑93 (MQ=255)
                                                                      gCGTTTCTGACTGATCCCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAAttt                  <  2:774349/93‑1 (MQ=255)
                                                                      gCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAAttt                  >  2:510458/1‑93 (MQ=255)
                                                                      gCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAAttt                  <  2:4319239/93‑1 (MQ=255)
                                                                        gTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCa                <  1:321783/93‑1 (MQ=255)
                                                                          ttCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACt              <  1:6191323/93‑1 (MQ=255)
                                                                          ttCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACt              >  1:1353012/1‑93 (MQ=255)
                                                                            ctgactgaTACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGc            <  2:2376792/93‑1 (MQ=255)
                                                                               actgaTACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTc         <  2:1706559/93‑1 (MQ=255)
                                                                               actgaTACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTc         <  1:1150284/93‑1 (MQ=255)
                                                                                ctgaTACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTcc        <  1:5981373/93‑1 (MQ=255)
                                                                                    tACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTCCATGc    <  1:6355474/93‑1 (MQ=255)
                                                                                     aCCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTCCATGcc   <  2:1965703/93‑1 (MQ=255)
                                                                                     aCCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTCCATGcc   >  2:1329615/1‑93 (MQ=255)
                                                                                      ccGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTCCATGCCt  >  1:5631918/1‑93 (MQ=255)
                                                                                         |                                                                                         
GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTCCATGCCT  >  NC_000913/1996525‑1996703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTCCATGCCTTCGT  >  NC_000913/1996524‑1996707
                                                                                          |                                                                                             
TGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAA                                                                                            >  SRR4302537.5953997/1‑94 (MQ=60)
 GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAAT                                                                                           <  SRR4302537.4038962/94‑1 (MQ=60)
 GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAAT                                                                                           <  SRR4302537.1437556/94‑1 (MQ=60)
 GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAAT                                                                                           >  SRR4302537.2071239/1‑94 (MQ=60)
  GGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATC                                                                                          <  SRR4302537.280228/94‑1 (MQ=60)
   GGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCA                                                                                         >  SRR4302537.3443339/1‑94 (MQ=60)
   GGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCA                                                                                         >  SRR4302537.2688718/1‑94 (MQ=60)
   GGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCA                                                                                         >  SRR4302537.6255761/1‑94 (MQ=60)
   GGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCA                                                                                         <  SRR4302537.5602199/94‑1 (MQ=60)
    GATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAG                                                                                        <  SRR4302537.4060148/94‑1 (MQ=60)
    GATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAG                                                                                        <  SRR4302537.5211971/94‑1 (MQ=60)
     ATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGC                                                                                       >  SRR4302537.3208320/1‑94 (MQ=60)
      TGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCC                                                                                      >  SRR4302537.1027547/1‑94 (MQ=60)
       GATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAGTCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCC                                                                                     <  SRR4302537.3807931/94‑1 (MQ=60)
        ATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCC                                                                                    <  SRR4302537.5741059/94‑1 (MQ=60)
         TAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCG                                                                                   <  SRR4302537.630209/94‑1 (MQ=60)
          AGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGC                                                                                  >  SRR4302537.240557/1‑94 (MQ=60)
             CGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCAT                                                                               >  SRR4302537.3632077/1‑94 (MQ=60)
               AACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCG                                                                             <  SRR4302537.173161/94‑1 (MQ=60)
               AACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCG                                                                             >  SRR4302537.839493/1‑94 (MQ=60)
                   GCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTT                                                                         <  SRR4302537.4297343/94‑1 (MQ=60)
                    CGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTT                                                                        >  SRR4302537.3098381/1‑94 (MQ=60)
                       AGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCG                                                                     >  SRR4302537.1690409/1‑94 (MQ=60)
                       AGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCG                                                                     >  SRR4302537.5088260/1‑94 (MQ=60)
                         GCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGT                                                                   >  SRR4302537.1345614/1‑94 (MQ=60)
                         GCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGT                                                                   <  SRR4302537.6329448/94‑1 (MQ=60)
                         GCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGT                                                                   <  SRR4302537.1835280/94‑1 (MQ=60)
                            CGCCGCCGGTATCAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATA                                                                <  SRR4302537.3666356/94‑1 (MQ=60)
                            CACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATA                                                                >  SRR4302537.4368966/1‑94 (MQ=60)
                             ACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATAC                                                               >  SRR4302537.4848472/1‑94 (MQ=60)
                             ACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATAC                                                               <  SRR4302537.5707430/94‑1 (MQ=60)
                               CGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACAC                                                             <  SRR4302537.671975/94‑1 (MQ=60)
                                GCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACA                                                            >  SRR4302537.4493763/1‑94 (MQ=60)
                                  CGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGG                                                          <  SRR4302537.5090383/94‑1 (MQ=60)
                                  CGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGG                                                          >  SRR4302537.98168/1‑94 (MQ=60)
                                  CGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGG                                                          >  SRR4302537.2838351/1‑94 (MQ=60)
                                   GGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGA                                                         >  SRR4302537.2046652/1‑94 (MQ=60)
                                    GTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGAT                                                        <  SRR4302537.4726089/94‑1 (MQ=60)
                                    GTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGAT                                                        <  SRR4302537.1797376/94‑1 (MQ=60)
                                    GTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGAT                                                        >  SRR4302537.6331857/1‑94 (MQ=60)
                                    GTATGAATAAACAGAATCGCCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGAT                                                        <  SRR4302537.2846165/94‑1 (MQ=60)
                                     TATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATC                                                       <  SRR4302537.4452364/94‑1 (MQ=60)
                                        GAATAAACAGAATCGTCCCTTCATCTTTGAAGCGTTGCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAG                                                    <  SRR4302537.1005225/94‑1 (MQ=60)
                                        GAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAG                                                    >  SRR4302537.6067034/1‑94 (MQ=60)
                                        GAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAG                                                    <  SRR4302537.287995/94‑1 (MQ=60)
                                        GAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAG                                                    <  SRR4302537.4647636/94‑1 (MQ=60)
                                        GAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACGGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAG                                                    <  SRR4302537.3419427/94‑1 (MQ=60)
                                         AATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGC                                                   <  SRR4302537.3497091/94‑1 (MQ=60)
                                          ATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCA                                                  <  SRR4302537.2542262/94‑1 (MQ=60)
                                           TAAACAGAATCGGCCCTTCATCTTTGAAGCGCTTCTGCCTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCACGCAG                                                 >  SRR4302537.3603275/1‑94 (MQ=60)
                                            AAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGA                                                >  SRR4302537.201366/1‑94 (MQ=60)
                                             AACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAA                                               >  SRR4302537.3808154/1‑94 (MQ=60)
                                             AACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAA                                               >  SRR4302537.3015789/1‑94 (MQ=60)
                                              ACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAAT                                              <  SRR4302537.4837788/94‑1 (MQ=60)
                                              ACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAAT                                              <  SRR4302537.4645303/94‑1 (MQ=60)
                                              ACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAAT                                              <  SRR4302537.5471666/94‑1 (MQ=60)
                                               CAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATG                                             <  SRR4302537.1604839/94‑1 (MQ=60)
                                               CAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATG                                             <  SRR4302537.1479379/94‑1 (MQ=60)
                                                AGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGC                                            >  SRR4302537.1464627/1‑94 (MQ=60)
                                                 GAATCGTCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCC                                           <  SRR4302537.598961/94‑1 (MQ=60)
                                                  AATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCT                                          >  SRR4302537.3041970/1‑94 (MQ=60)
                                                     CTGCCCTTCATCTTTCAAGCGTTTCTGACTGATACCGCCAATCACCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCC                                       <  SRR4302537.566441/94‑1 (MQ=60)
                                                       GCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAG                                     <  SRR4302537.135694/94‑1 (MQ=60)
                                                        CCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGC                                    >  SRR4302537.4071536/1‑94 (MQ=60)
                                                        CCCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGC                                    >  SRR4302537.4889879/1‑94 (MQ=60)
                                                         CCTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCC                                   <  SRR4302537.3366101/94‑1 (MQ=60)
                                                          CTTCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCG                                  <  SRR4302537.2441379/94‑1 (MQ=60)
                                                            TCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCG                                >  SRR4302537.2931154/1‑94 (MQ=60)
                                                            TCATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCG                                <  SRR4302537.3576712/94‑1 (MQ=60)
                                                            TCATCTTTGAAGCGTTCCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCG                                >  SRR4302537.2567095/1‑94 (MQ=60)
                                                              ATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCC                              >  SRR4302537.5591749/1‑94 (MQ=60)
                                                              ATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCC                              >  SRR4302537.6253978/1‑94 (MQ=60)
                                                              ATCTTTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCC                              >  SRR4302537.4718850/1‑94 (MQ=60)
                                                                  TTGAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCA                          <  SRR4302537.5233513/94‑1 (MQ=60)
                                                                    GAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAAT                        <  SRR4302537.1241696/94‑1 (MQ=60)
                                                                    GAAGCGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAAT                        >  SRR4302537.3843194/1‑94 (MQ=60)
                                                                        CGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCA                    <  SRR4302537.1764435/94‑1 (MQ=60)
                                                                        CGTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCA                    >  SRR4302537.2244031/1‑94 (MQ=60)
                                                                         GTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCAC                   >  SRR4302537.5325543/1‑94 (MQ=60)
                                                                         GTTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCAC                   <  SRR4302537.324011/94‑1 (MQ=60)
                                                                          TTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACT                  <  SRR4302537.3515065/94‑1 (MQ=60)
                                                                          TTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACT                  >  SRR4302537.4669112/1‑94 (MQ=60)
                                                                          TTTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACT                  >  SRR4302537.1362436/1‑94 (MQ=60)
                                                                           TTCTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTG                 <  SRR4302537.6233977/94‑1 (MQ=60)
                                                                             CTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCT               <  SRR4302537.5838971/94‑1 (MQ=60)
                                                                             CTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCT               >  SRR4302537.5667179/1‑94 (MQ=60)
                                                                             CTGACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCT               >  SRR4302537.5532836/1‑94 (MQ=60)
                                                                                ACTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTCC            <  SRR4302537.1158281/94‑1 (MQ=60)
                                                                                 CTGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTCCA           <  SRR4302537.6022433/94‑1 (MQ=60)
                                                                                  TGATACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTCCAT          >  SRR4302537.3845885/1‑94 (MQ=60)
                                                                                     TACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTCCATGCC       <  SRR4302537.6398856/94‑1 (MQ=60)
                                                                                      ACCGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTCCATGCCT      >  SRR4302537.5670449/1‑94 (MQ=60)
                                                                                        CGCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTCCATGCCTTC    >  SRR4302537.6457613/1‑94 (MQ=60)
                                                                                         GCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTCCATGCCTTCG   <  SRR4302537.3816528/94‑1 (MQ=60)
                                                                                         GCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTCCATGCCTTCG   >  SRR4302537.4196679/1‑94 (MQ=60)
                                                                                         GCCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTCCATGCCTTCG   <  SRR4302537.1254244/94‑1 (MQ=60)
                                                                                         GCCAATCAGCCACGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTCCATGCCTTCG   <  SRR4302537.4489927/94‑1 (MQ=60)
                                                                                          CCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTCCATGCCTTCGT  >  SRR4302537.4814838/1‑94 (MQ=60)
                                                                                          |                                                                                             
TGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTCCATGCCTTCGT  >  NC_000913/1996524‑1996707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑