Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	647	270.1	21258240	98.4%	20918108	94.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,008,480	+A	coding (637/654 nt)	yedK →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,008,476	1	.	A	96.7%	813.0 / 15.1	215	coding (633/654 nt)	yedK	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (2/5); new base A (94/114); total (96/119)
Fisher's exact test for biased strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 236 total aligned reads displayed.

TAGCGGTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑A‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTAG  >  NC_000913/2008383‑2008564

 aGCGGTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑a                                                                                           <  2:2579577/94‑1 (MQ=255)

 aGCGGTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑a                                                                                           >  1:7899690/1‑94 (MQ=255)

 aGCGGTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑a                                                                                           <  2:2606367/94‑1 (MQ=255)

  gCGGTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑a‑a                                                                                         <  1:1102795/94‑1 (MQ=255)

    gttAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGGTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑a‑aaa                                                                                       <  1:7345238/92‑1 (MQ=255)

    ggTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑a‑aaa                                                                                       <  2:5850111/94‑1 (MQ=255)

    ggTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑a‑aaa                                                                                       >  1:3030509/1‑94 (MQ=255)

     gTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑a‑aaaa                                                                                      <  1:6477810/94‑2 (MQ=255)

      tAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑a‑aaaac                                                                                     <  2:9071994/94‑3 (MQ=255)

       aaaGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑a‑aacacc                                                                                    >  2:7060902/1‑90 (MQ=255)

       aaaGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑a‑aaaacc                                                                                    <  2:5333588/94‑4 (MQ=255)

          gaagCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAgg                                                                                 <  2:5655257/94‑1 (MQ=255)

          gaagCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAgg                                                                                 <  2:5795523/94‑1 (MQ=255)

          gaagCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAgg                                                                                 <  2:5025076/94‑1 (MQ=255)

           aagCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAggg                                                                                >  2:5110495/1‑94 (MQ=255)

            agCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTGTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGgc                                                                               <  2:3451617/94‑1 (MQ=255)

            agCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGgc                                                                               <  1:2851587/94‑1 (MQ=255)

            agCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGgc                                                                               >  1:9395869/1‑94 (MQ=255)

            agCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGgc                                                                               >  1:3788482/1‑94 (MQ=255)

             gCCTCAGAAAGCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGgcg                                                                              <  2:4186196/94‑1 (MQ=255)

             accTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGgcg                                                                              <  2:6314112/93‑1 (MQ=255)

              ccTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGgcgc                                                                             >  1:4162831/1‑94 (MQ=255)

               cTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGcgcg                                                                            >  2:3929489/1‑94 (MQ=255)

               cTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGcgcg                                                                            >  2:1464333/1‑94 (MQ=255)

                 cAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCggg                                                                          >  1:7607226/1‑93 (MQ=255)

                 cAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGa                                                                          <  2:1256443/94‑1 (MQ=255)

                 cAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGa                                                                          <  1:6388176/94‑1 (MQ=255)

                  aGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAg                                                                         <  2:9601613/94‑1 (MQ=255)

                  aGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAg                                                                         <  1:7957951/94‑1 (MQ=255)

                   gAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGt                                                                        <  2:6755681/94‑1 (MQ=255)

                    aaaTCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGtt                                                                       >  2:6013303/1‑94 (MQ=255)

                    aaaTCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGtt                                                                       <  1:10021542/94‑1 (MQ=255)

                    aaaTCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGtt                                                                       <  1:699735/94‑1 (MQ=255)

                     aaTCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTa                                                                      <  2:790098/94‑1 (MQ=255)

                     aaTCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTa                                                                      <  2:8750876/94‑1 (MQ=255)

                       tCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAAt                                                                    <  1:4780519/94‑1 (MQ=255)

                       acgcgGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAAt                                                                    <  2:3390157/93‑1 (MQ=255)

                        cgcgGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAAtt                                                                   <  1:3221390/94‑1 (MQ=255)

                        cgcgGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑A‑AAA‑CCAGGGCGCGGAGTTAATTc                                                                  <  1:3430468/94‑1 (MQ=255)

                          cgGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCa                                                                 <  1:6876576/94‑1 (MQ=255)

                              cAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCt                                                             <  2:10594421/94‑1 (MQ=255)

                              cAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCt                                                             >  2:10265575/1‑94 (MQ=255)

                               aGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCtt                                                            >  2:1971151/1‑93 (MQ=255)

                               aGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGGTAATTCAACCTg                                                            >  2:4282689/1‑94 (MQ=255)

                                 tGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGtt                                                          >  2:2677186/1‑94 (MQ=255)

                                 tGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGtt                                                          >  2:6132214/1‑94 (MQ=255)

                                  tgCTTTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGttt                                                         <  1:8890652/93‑1 (MQ=255)

                                  ggCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGttt                                                         <  1:8890649/94‑1 (MQ=255)

                                  ggCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGttt                                                         >  2:6680758/1‑94 (MQ=255)

                                   gCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTg                                                        <  2:3671933/94‑1 (MQ=255)

                                    cTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGa                                                       >  2:847128/1‑94 (MQ=255)

                                    cTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGa                                                       <  1:10386769/94‑1 (MQ=255)

                                    cTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑ACAAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGa                                                       >  2:9623446/1‑94 (MQ=255)

                                     tgtgTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAACGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGAt                                                      >  2:9521968/1‑94 (MQ=255)

                                      gtgtTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑A‑AAACCCAGGGCGCGGAGTTAATTCAACCTGTTTGATa                                                     <  1:2665173/94‑1 (MQ=255)

                                       tgtTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATaa                                                    >  1:818568/1‑94 (MQ=255)

                                       tgtTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATaa                                                    >  1:8611149/1‑94 (MQ=255)

                                       tgtTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATaa                                                    >  2:818225/1‑94 (MQ=255)

                                           ccAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCt                                                <  1:4991932/94‑1 (MQ=255)

                                            cAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTg                                               <  1:3512132/94‑1 (MQ=255)

                                            cAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTg                                               <  2:5094835/94‑1 (MQ=255)

                                             aGCAAACCAGTTCTCCTGGCGCCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGTCTgg                                              <  1:1891121/94‑1 (MQ=255)

                                             aGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTgg                                              >  1:771775/1‑94 (MQ=255)

                                               cAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGgag                                            <  2:7009638/94‑1 (MQ=255)

                                               cAAACCAGTTCGACTGGCGCCAGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGgag                                            <  1:10571352/94‑1 (MQ=255)

                                                aaaCCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGgaga                                           >  1:4682121/1‑94 (MQ=255)

                                                aaaCCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGgaga                                           >  2:10083698/1‑94 (MQ=255)

                                                 aaCCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGat                                          <  2:8537179/94‑1 (MQ=255)

                                                        tctcCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGAt                                   <  1:7828773/94‑1 (MQ=255)

                                                         ctcCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATc                                  >  1:2385658/1‑94 (MQ=255)

                                                         ctcCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATc                                  <  1:5787571/94‑1 (MQ=255)

                                                         ctcCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATc                                  <  2:8922620/94‑1 (MQ=255)

                                                         ctcCTGGCACCCGGTATCGCGCGCGGTGGGT‑ATGTTAA‑AAA‑CCAAGGCGCCGAGGTAATTCAACCTGGTTGATAAGCCTGGAGATTATTGATca                                 >  2:9874481/1‑94 (MQ=255)

                                                          tcCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATca                                 >  2:4524336/1‑94 (MQ=255)

                                                          tcCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATca                                 <  2:736726/94‑1 (MQ=255)

                                                           ccTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATcaa                                <  1:8777364/94‑1 (MQ=255)

                                                           ccTGGCACCCGGTATCGCGAGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATcaa                                <  1:5393183/94‑1 (MQ=255)

                                                              ggCACCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATcaacaa                             >  2:8416448/1‑94 (MQ=255)

                                                                 ccccGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATAc                          <  2:2364978/93‑1 (MQ=255)

                                                                 aCCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATAc                          >  1:166877/1‑94 (MQ=255)

                                                                 aCCCGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATAc                          <  2:460612/94‑1 (MQ=255)

                                                                  cccGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACt                         <  2:1679028/94‑1 (MQ=255)

                                                                  cccGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACt                         >  1:9813553/1‑94 (MQ=255)

                                                                  cccGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACt                         >  2:6803990/1‑94 (MQ=255)

                                                                    cGGTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGc                       <  2:2917794/94‑1 (MQ=255)

                                                                     ggTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCg                      <  1:710457/94‑1 (MQ=255)

                                                                     ggTATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCg                      >  1:8543471/1‑94 (MQ=255)

                                                                       tATCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTc                    >  1:3384604/1‑94 (MQ=255)

                                                                        aTCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCa                   >  1:9234225/1‑94 (MQ=255)

                                                                        aTCGCGCGCGGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCa                   >  1:6280253/1‑94 (MQ=255)

                                                                          cgcgcgcgGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATa                 <  1:5688186/94‑1 (MQ=255)

                                                                            cgcgcgGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAg               <  1:6660997/94‑1 (MQ=255)

                                                                               gcgGTGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGaaa            >  1:5243627/1‑94 (MQ=255)

                                                                                   tGGGTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAAtctc        >  2:2780990/1‑94 (MQ=255)

                                                                                    gggTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATctct       <  1:1290530/94‑1 (MQ=255)

                                                                                    gggTAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATctct       <  2:2207890/94‑1 (MQ=255)

                                                                                       tAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTAtt    <  1:8305389/94‑1 (MQ=255)

                                                                                       tAATGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTAtt    <  2:6260971/94‑1 (MQ=255)

                                                                                        aaTGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTa   <  1:9531783/94‑1 (MQ=255)

                                                                                         aTGTTAA‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTAg  <  2:9813553/94‑1 (MQ=255)

TAGCGGTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑A‑AAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTAG  >  NC_000913/2008383‑2008564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GGTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TT‑AA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTAGACAAAGATT  >  NC_000913/2008387‑2008573

GTTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGGTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TT‑AA‑AA                                                                                                <  SRR4302545.7551502/94‑1 (MQ=60)

GGTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TT‑AA‑AA                                                                                                >  SRR4302545.3104685/1‑94 (MQ=60)

 GTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TT‑AA‑AA‑A                                                                                              >  SRR4302545.6889414/1‑94 (MQ=60)

 GTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TT‑AA‑AA‑A                                                                                              <  SRR4302545.6654907/94‑1 (MQ=60)

  TAAAGAAGCCTCAGAAATAGCGGCCAGCGGCTGTGTTCCAGCAAACCAGTTCTCCTCGCACCCGGTATCGCGCGCGGTGGGGAATG‑TT‑AA‑AA‑AC                                                                                             >  SRR4302545.588853/1‑94 (MQ=60)

       AAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGG                                                                                         >  SRR4302545.5075072/1‑94 (MQ=60)

        AGCCTCAGAAATCGCGGCTAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGC                                                                                        >  SRR4302545.5878736/1‑94 (MQ=60)

        AGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGC                                                                                        <  SRR4302545.2920528/94‑1 (MQ=60)

        AGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGC                                                                                        >  SRR4302545.3882300/1‑94 (MQ=60)

        AGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGC                                                                                        >  SRR4302545.5878735/1‑94 (MQ=60)

        AGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGC                                                                                        >  SRR4302545.9671675/1‑94 (MQ=60)

          CCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGC                                                                                      >  SRR4302545.4266744/1‑94 (MQ=60)

             CAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGG                                                                                   >  SRR4302545.7822414/1‑94 (MQ=60)

             CAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGA                                                                                   <  SRR4302545.6562849/94‑1 (MQ=60)

             CAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTAGCACCCGGTATCGCGCGCGGTGGGCAATG‑TTAAA‑AA‑CCAGGGCGCGGG                                                                                   >  SRR4302545.7855325/1‑94 (MQ=60)

              AGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAG                                                                                  <  SRR4302545.8444014/94‑1 (MQ=60)

              AGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAG                                                                                  <  SRR4302545.8184130/94‑1 (MQ=60)

              AGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAG                                                                                  <  SRR4302545.8184129/94‑1 (MQ=60)

              AGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAG                                                                                  <  SRR4302545.372169/94‑1 (MQ=60)

               GAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGT                                                                                 >  SRR4302545.2982581/1‑94 (MQ=60)

                AAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTT                                                                                >  SRR4302545.1711817/1‑94 (MQ=60)

                AAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTT                                                                                >  SRR4302545.8195635/1‑94 (MQ=60)

                AAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTT                                                                                >  SRR4302545.6480959/1‑94 (MQ=60)

                AAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTT                                                                                <  SRR4302545.715418/94‑1 (MQ=60)

                AAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTT                                                                                <  SRR4302545.10318555/94‑1 (MQ=60)

                  ATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAA                                                                              <  SRR4302545.10694270/94‑1 (MQ=60)

                   TCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAAT                                                                             <  SRR4302545.4902744/94‑1 (MQ=60)

                    CGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATT                                                                            <  SRR4302545.3300431/94‑1 (MQ=60)

                    CGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TT‑AA‑AA‑CCAGGGCGCGGAGTTAATTC                                                                           <  SRR4302545.3514310/94‑1 (MQ=60)

                      CGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCA                                                                          <  SRR4302545.7065569/94‑1 (MQ=60)

                       GGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAA                                                                         >  SRR4302545.5242461/1‑94 (MQ=60)

                           AGTGGCTGTGTTCCAGCAAACCAGTTCTCCTAGCGCCCGGTATCGGGCGCGGTGGATAATT‑TTAAA‑AA‑CCAGGTCGCGGAGTTAATACAACCTG                                                                     <  SRR4302545.9790173/94‑1 (MQ=57)

                              tgcttTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTT                                                                  <  SRR4302545.9149045/89‑1 (MQ=60)

                              GGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTT                                                                  <  SRR4302545.9149043/94‑1 (MQ=60)

                              GGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTT                                                                  <  SRR4302545.9149042/94‑1 (MQ=60)

                               GCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTG                                                                 <  SRR4302545.3545195/94‑1 (MQ=60)

                                CTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGA                                                                <  SRR4302545.10692975/94‑1 (MQ=60)

                                 TGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGGTAATTCAACCTGTTTGAT                                                               >  SRR4302545.7810289/1‑94 (MQ=60)

                                  GTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATA                                                              <  SRR4302545.2729386/94‑1 (MQ=60)

                                  GTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TT‑AA‑AACCCAGGGCGCGGAGTTAATTCAACCTGTTTGATA                                                              <  SRR4302545.2729385/94‑1 (MQ=60)

                                   TGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAA                                                             >  SRR4302545.836914/1‑94 (MQ=60)

                                   TGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAA                                                             >  SRR4302545.8860387/1‑94 (MQ=60)

                                    GTTGCAGCAATCCAGTCCTCCTGGCACCGGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGAGCGGAGTTAATTCAACCTGTTTGATAAG                                                            <  SRR4302545.7509954/94‑1 (MQ=60)

                                     TTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGC                                                           <  SRR4302545.712514/94‑1 (MQ=60)

                                     TTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGC                                                           <  SRR4302545.2699986/94‑1 (MQ=60)

                                       CCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCT                                                         <  SRR4302545.5118351/94‑1 (MQ=60)

                                        CAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTG                                                        <  SRR4302545.7213028/94‑1 (MQ=60)

                                        CAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTG                                                        >  SRR4302545.5194426/1‑94 (MQ=60)

                                        CAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTG                                                        <  SRR4302545.3598272/94‑1 (MQ=60)

                                        CAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTG                                                        >  SRR4302545.878652/1‑94 (MQ=60)

                                         AGCAAACCAGTTCTCCTGGCGCCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGTCTGG                                                       <  SRR4302545.1937705/94‑1 (MQ=60)

                                         AGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGG                                                       <  SRR4302545.1937706/94‑1 (MQ=60)

                                         AGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGG                                                       >  SRR4302545.8621681/1‑94 (MQ=60)

                                         AGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGG                                                       >  SRR4302545.789121/1‑94 (MQ=60)

                                           CAAACCAGTTCGACTGGCGCCAGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAG                                                     <  SRR4302545.10882759/94‑1 (MQ=60)

                                           CAAACAAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TT‑AA‑AATCCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAG                                                     <  SRR4302545.435545/94‑1 (MQ=60)

                                            AAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGA                                                    >  SRR4302545.4801535/1‑94 (MQ=60)

                                            AAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGA                                                    >  SRR4302545.112540/1‑94 (MQ=60)

                                             AACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGAT                                                   >  SRR4302545.6515457/1‑94 (MQ=60)

                                                 AGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATT                                               >  SRR4302545.1041156/1‑94 (MQ=60)

                                                 AGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATT                                               <  SRR4302545.3942359/94‑1 (MQ=60)

                                                 AGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATT                                               <  SRR4302545.344198/94‑1 (MQ=60)

                                                    TCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGAT                                            <  SRR4302545.8050624/94‑1 (MQ=60)

                                                     CTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATC                                           <  SRR4302545.5943196/94‑1 (MQ=60)

                                                     CTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATC                                           >  SRR4302545.1901483/1‑94 (MQ=60)

                                                     CTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATC                                           >  SRR4302545.2443536/1‑94 (MQ=60)

                                                       CCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAA                                         >  SRR4302545.9429740/1‑94 (MQ=60)

                                                       CCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAA                                         <  SRR4302545.9032073/94‑1 (MQ=60)

                                                       CCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAA                                         >  SRR4302545.9703051/1‑94 (MQ=60)

                                                       CCTGGCACCCGGTATCGCGCGCGGTGGGAAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATGAA                                         >  SRR4302545.286707/1‑94 (MQ=60)

                                                       CCTGGCACCCGGTATCGCGAGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAA                                         <  SRR4302545.5533679/94‑1 (MQ=60)

                                                         TGGCGCCCGGTATCGCGCGCGGTGGGTAATG‑TT‑AA‑AACCCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACA                                       <  SRR4302545.6654248/94‑1 (MQ=60)

                                                          GGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAA                                      <  SRR4302545.9606808/94‑1 (MQ=60)

                                                           GCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAAT                                     >  SRR4302545.8103544/1‑94 (MQ=60)

                                                           GCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAAT                                     >  SRR4302545.10225315/1‑94 (MQ=60)

                                                            CACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATA                                    <  SRR4302545.10294698/94‑1 (MQ=60)

                                                            CACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATA                                    >  SRR4302545.1115522/1‑94 (MQ=60)

                                                            CACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAACA                                    >  SRR4302545.6957741/1‑94 (MQ=60)

                                                             ACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATAC                                   <  SRR4302545.1147059/94‑1 (MQ=60)

                                                             ACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATAC                                   >  SRR4302545.170442/1‑94 (MQ=60)

                                                             ACCCGGGACCGCGCGCGGTGGGTAATGCTA‑AA‑AA‑CCAGGGCGCGGAGTTAAGACAACCTGTTTGATAAGCCTGGAGATTATTGACCAACAACAC                                   >  SRR4302545.9640794/1‑94 (MQ=60)

                                                              CCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACT                                  >  SRR4302545.10105143/1‑94 (MQ=60)

                                                              CCCGGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACT                                  <  SRR4302545.9065610/94‑1 (MQ=60)

                                                                 GGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCG                               <  SRR4302545.726418/94‑1 (MQ=60)

                                                                 GGTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCG                               >  SRR4302545.8790482/1‑94 (MQ=60)

                                                                  GTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGT                              >  SRR4302545.3591574/1‑94 (MQ=60)

                                                                  GTATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGT                              >  SRR4302545.997753/1‑94 (MQ=60)

                                                                   TATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTC                             >  SRR4302545.3467078/1‑94 (MQ=60)

                                                                    ATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCA                            >  SRR4302545.6451455/1‑94 (MQ=60)

                                                                    ATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCA                            >  SRR4302545.9504955/1‑94 (MQ=60)

                                                                    ATCGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCA                            >  SRR4302545.6451459/1‑94 (MQ=60)

                                                                      CGCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATA                          <  SRR4302545.5840336/94‑1 (MQ=60)

                                                                       GCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAA                         <  SRR4302545.4815903/94‑1 (MQ=60)

                                                                       GCGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAA                         <  SRR4302545.8772011/94‑1 (MQ=60)

                                                                        CGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAG                        <  SRR4302545.6843634/94‑1 (MQ=60)

                                                                        CGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAG                        <  SRR4302545.4736352/94‑1 (MQ=60)

                                                                        CGCGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAG                        >  SRR4302545.560213/1‑94 (MQ=60)

                                                                        CACGCGGTGGTTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAAATCAACCTGTTTGATAATCATGGAGATGATTGAGCACCAATTCTTCGTCATAAG                        <  SRR4302545.7597614/94‑1 (MQ=32)

                                                                          CGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAA                      >  SRR4302545.7958223/1‑94 (MQ=60)

                                                                          CGCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAA                      >  SRR4302545.7958221/1‑94 (MQ=60)

                                                                           GCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAA                     >  SRR4302545.3377692/1‑94 (MQ=60)

                                                                           GCGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAA                     >  SRR4302545.5378975/1‑94 (MQ=60)

                                                                            GGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAAT                    <  SRR4302545.6835164/94‑1 (MQ=60)

                                                                            CGGTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAAT                    <  SRR4302545.350224/94‑1 (MQ=60)

                                                                              GTGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCT                  >  SRR4302545.322954/1‑94 (MQ=60)

                                                                               TGGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTC                 >  SRR4302545.7498919/1‑94 (MQ=60)

                                                                                GGGTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCT                <  SRR4302545.1319208/94‑1 (MQ=60)

                                                                                  GTAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTAT              <  SRR4302545.156473/94‑1 (MQ=60)

                                                                                   TAATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATT             <  SRR4302545.8546007/94‑1 (MQ=60)

                                                                                    AATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTA            <  SRR4302545.9812455/94‑1 (MQ=60)

                                                                                    AATG‑TTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTA            <  SRR4302545.5524886/94‑1 (MQ=60)

                                                                                       G‑TT‑AACAA‑CCAGGGCGCGGAGTTAATTCAACCTGCTTGATAAGACTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTAGAC         >  SRR4302545.1212344/1‑94 (MQ=60)

                                                                                         TG‑AA‑AA‑CCCGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTAGACAA       >  SRR4302545.6650111/1‑94 (MQ=60)

                                                                                         TA‑AA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTAGACAA       >  SRR4302545.9103147/1‑94 (MQ=60)

                                                                                          A‑AA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTAGACAAA      <  SRR4302545.7012180/94‑1 (MQ=60)

                                                                                            AA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTAGACAAAG     >  SRR4302545.6331669/1‑94 (MQ=60)

                                                                                            AA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTAGACAAAG     >  SRR4302545.2735204/1‑94 (MQ=60)

                                                                                            AA‑AA‑CAAGGGCGCGGAGTTAGTTCATCCTGTTTGATAAGCCTGGAGATTATTGATCATCAATCCGGCGTCATAAGAAATCTCTATTAGACAAAG     <  SRR4302545.7182642/94‑1 (MQ=60)

                                                                                               AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTAGACAAAGAT   >  SRR4302545.7141247/1‑94 (MQ=60)

                                                                                               AA‑CCAGGGCGCAGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAGCAATACTGCGTCATAAGAAATCTCTATTAGACAAAGAT   <  SRR4302545.4823968/94‑1 (MQ=60)

                                                                                                A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTAGACAAAGATT  >  SRR4302545.6961321/1‑94 (MQ=60)

GGTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TT‑AA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTAGACAAAGATT  >  NC_000913/2008387‑2008573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`