Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,012,544 A→G S432G (AGT→GGT)  fliF → FliF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,012,5440AG98.7% 785.4 / ‑2.2 229S432G (AGT→GGT) fliFFliF
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base G (108/118);  total (110/119)
Fisher's exact test for biased strand distribution p-value = 6.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 236 total aligned reads displayed.

GAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTGC  >  NC_000913/2012453‑2012634
                                                                                           |                                                                                          
gAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGt                                                                                          <  1:2332238/94‑1 (MQ=255)
gAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGt                                                                                          <  1:500951/94‑1 (MQ=255)
gAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGt                                                                                          >  1:473111/1‑94 (MQ=255)
 aaGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTg                                                                                         <  2:9534461/94‑1 (MQ=255)
 aaGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGGTAACTCGCCGTTCAATAGCGGTg                                                                                         >  1:5919578/1‑94 (MQ=255)
  aGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGa                                                                                        >  2:4644677/1‑94 (MQ=255)
   gCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGAc                                                                                       <  2:5781541/94‑1 (MQ=255)
     aaaTTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGa                                                                                     >  2:6246835/1‑94 (MQ=255)
       aTTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGaaa                                                                                   <  2:5111490/94‑1 (MQ=255)
       aTTGAAGATCTGACCCGCGAGGCGATGGGCGTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGaaa                                                                                   >  2:9482368/1‑94 (MQ=255)
        ttGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAg                                                                                  <  2:2584103/94‑1 (MQ=255)
         tGAAGATTTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGAAGAAAgc                                                                                 >  1:7410306/1‑94 (MQ=255)
          gAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAgcg                                                                                >  2:1160504/1‑94 (MQ=255)
           aaGATCTGACCCGCGAGGCTATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGCCGAAAgcgc                                                                               >  2:7127000/1‑93 (MQ=255)
            aGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAgcggc                                                                              <  1:633132/94‑1 (MQ=255)
               tCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGcggcggg                                                                           >  2:2225901/1‑93 (MQ=255)
               tCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGga                                                                           <  1:9873015/94‑1 (MQ=255)
               tCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGga                                                                           <  2:8871335/94‑1 (MQ=255)
                cTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGgag                                                                          >  2:7064988/1‑94 (MQ=255)
                cTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGgag                                                                          >  1:4881089/1‑94 (MQ=255)
                 tGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGgaga                                                                         >  1:814004/1‑94 (MQ=255)
                 tGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGgaga                                                                         >  2:8701378/1‑94 (MQ=255)
                  gACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGaa                                                                        <  2:5039897/94‑1 (MQ=255)
                  gACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGaa                                                                        >  1:8497131/1‑94 (MQ=255)
                    cccGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACt                                                                      <  1:6009974/94‑1 (MQ=255)
                     ccGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTg                                                                     >  2:7867601/1‑94 (MQ=255)
                         gAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCAt                                                                 >  1:5986167/1‑94 (MQ=255)
                         gAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCAt                                                                 >  1:5047719/1‑94 (MQ=255)
                          aGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCAtt                                                                >  2:10049176/1‑94 (MQ=255)
                          aGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCAtt                                                                <  2:7710395/94‑1 (MQ=255)
                           ggCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTc                                                               >  2:7570457/1‑94 (MQ=255)
                            gCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCt                                                              <  2:1048898/94‑1 (MQ=255)
                            gCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCt                                                              <  1:1454744/94‑1 (MQ=255)
                             cGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTg                                                             <  2:2507243/94‑1 (MQ=255)
                             cGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTg                                                             <  1:3860336/94‑1 (MQ=255)
                             cGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTg                                                             >  1:4514909/1‑94 (MQ=255)
                               aTGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGc                                                           <  2:6395289/94‑1 (MQ=255)
                                tGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCa                                                          >  2:4593942/1‑94 (MQ=255)
                                tGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCa                                                          <  2:10128029/94‑1 (MQ=255)
                                tGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCAGTGACGTAAGCGGCGGAGAACTGCCCATCTGGCa                                                          >  2:8983831/1‑94 (MQ=255)
                                 gggCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCaa                                                         <  2:8432732/94‑1 (MQ=255)
                                   gCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACa                                                       >  1:8676205/1‑94 (MQ=255)
                                    cTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAg                                                      <  2:1908875/94‑1 (MQ=255)
                                    cTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAg                                                      >  1:5318222/1‑94 (MQ=255)
                                     ttttCTGAAAAACTCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGc                                                     <  2:7288916/94‑1 (MQ=255)
                                     ttttCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGc                                                     >  2:4449489/1‑94 (MQ=255)
                                      tttCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCa                                                    <  2:5881411/94‑1 (MQ=255)
                                      tttCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCa                                                    <  2:6186624/94‑1 (MQ=255)
                                       ttCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCaa                                                   <  2:8348434/94‑1 (MQ=255)
                                        tCTGAAAAACGCGGTGCCCCGCTCAATGTCTTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAg                                                  <  2:2974047/94‑1 (MQ=255)
                                        tCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAg                                                  <  1:6245022/94‑1 (MQ=255)
                                          tGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCg                                                >  2:1284102/1‑94 (MQ=255)
                                           gAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGt                                               <  2:6310634/94‑1 (MQ=255)
                                            aaaaaCGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGtt                                              <  2:1965982/94‑1 (MQ=255)
                                             aaaaCGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGttt                                             >  2:1470785/1‑94 (MQ=255)
                                              aaaCGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTa                                            >  2:6010138/1‑94 (MQ=255)
                                              aaaCGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTa                                            >  2:3687184/1‑94 (MQ=255)
                                               aaCGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTAt                                           <  2:10343522/94‑1 (MQ=255)
                                               aaCGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTAt                                           <  1:6878333/94‑1 (MQ=255)
                                               aaCGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTAt                                           >  1:2984572/1‑94 (MQ=255)
                                                aCGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATc                                          <  1:10154672/94‑1 (MQ=255)
                                                     gTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCa                                     <  1:2661391/94‑1 (MQ=255)
                                                     gTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCa                                     <  1:4169879/94‑1 (MQ=255)
                                                     gTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCa                                     <  2:4662034/94‑1 (MQ=255)
                                                       gACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGt                                   <  2:1377551/94‑1 (MQ=255)
                                                          tCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTAc                                >  1:5262885/1‑94 (MQ=255)
                                                          tCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTAc                                <  1:917735/94‑1 (MQ=255)
                                                           cgcgCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACt                               <  2:3438066/94‑1 (MQ=255)
                                                            gCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACtt                              >  2:7672277/1‑94 (MQ=255)
                                                            gCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACtt                              >  2:1165325/1‑94 (MQ=255)
                                                             cTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTtg                             <  1:8915090/94‑1 (MQ=255)
                                                              tCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAATTACTtgc                            >  1:6640459/1‑94 (MQ=255)
                                                               cAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTtgct                           <  2:1165425/94‑1 (MQ=255)
                                                               cAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTtgct                           <  2:5329694/94‑1 (MQ=255)
                                                                aaTGTCGTTAACTCGCCGTTCAATAGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTtgctg                          <  1:10502484/94‑1 (MQ=255)
                                                                 aTGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTtgctgc                         <  1:10426924/94‑1 (MQ=255)
                                                                 aTGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTtgctgc                         >  1:363519/1‑94 (MQ=255)
                                                                  tGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGcc                        <  1:9801276/94‑1 (MQ=255)
                                                                   gTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCg                       <  1:7890054/94‑1 (MQ=255)
                                                                   gTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCg                       >  2:10612149/1‑94 (MQ=255)
                                                                     cggtAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGt                     <  2:7549178/91‑1 (MQ=255)
                                                                     cGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGt                     >  1:1340551/1‑94 (MQ=255)
                                                                     cGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGt                     >  2:409718/1‑94 (MQ=255)
                                                                     cGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTACCGGt                     >  1:1174188/1‑94 (MQ=255)
                                                                      gTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTc                    <  1:5608663/94‑1 (MQ=255)
                                                                          aCTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTg                >  1:9263600/1‑94 (MQ=255)
                                                                           cTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTgg               >  1:7133659/1‑94 (MQ=255)
                                                                           cTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTgg               <  2:8256989/94‑1 (MQ=255)
                                                                             cGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGtt             <  2:10237750/94‑1 (MQ=255)
                                                                             cGCCGTCCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGtt             <  2:6722527/94‑1 (MQ=255)
                                                                              gCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTg            <  2:4864063/94‑1 (MQ=255)
                                                                               ccGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGc           >  1:1994627/1‑94 (MQ=255)
                                                                                cGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCt          >  1:1543013/1‑94 (MQ=255)
                                                                                 gTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTg         >  2:6122831/1‑94 (MQ=255)
                                                                                    cAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTa      >  1:4038096/1‑94 (MQ=255)
                                                                                     aaTAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTAc     >  2:4392833/1‑94 (MQ=255)
                                                                                     aaTAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTAc     >  1:4118598/1‑94 (MQ=255)
                                                                                      aTAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTAct    >  1:1223875/1‑94 (MQ=255)
                                                                                       tAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTActg   <  1:7017911/94‑1 (MQ=255)
                                                                                        aGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTActgc  <  2:5405146/94‑1 (MQ=255)
                                                                                           |                                                                                          
GAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTGC  >  NC_000913/2012453‑2012634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTGCTGG  >  NC_000913/2012451‑2012637
                                                                                             |                                                                                             
ATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCG                                                                                               <  SRR4302545.10619148/94‑1 (MQ=60)
 TGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGG                                                                                              <  SRR4302545.5808668/94‑1 (MQ=60)
 TGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCCTTCAATAGCGG                                                                                              <  SRR4302545.7053834/94‑1 (MQ=60)
  GAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGT                                                                                             <  SRR4302545.512175/94‑1 (MQ=60)
  GAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGT                                                                                             <  SRR4302545.512174/94‑1 (MQ=60)
  GAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGT                                                                                             <  SRR4302545.2389038/94‑1 (MQ=60)
  GAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGT                                                                                             >  SRR4302545.483661/1‑94 (MQ=60)
   AAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGGTAACTCGCCGTTCAATAGCGGTG                                                                                            >  SRR4302545.6079246/1‑94 (MQ=60)
       AAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGA                                                                                        >  SRR4302545.4255125/1‑94 (MQ=60)
       AAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGA                                                                                        >  SRR4302545.5019034/1‑94 (MQ=60)
         ATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGGGACGAAA                                                                                      >  SRR4302545.2337797/1‑94 (MQ=60)
           TGAAGATTTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGAAGAAAGC                                                                                    >  SRR4302545.7619695/1‑94 (MQ=60)
           TGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGG                                                                                    >  SRR4302545.4157407/1‑94 (MQ=60)
           TGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGC                                                                                    <  SRR4302545.4453127/94‑1 (MQ=60)
           TGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGC                                                                                    >  SRR4302545.9538/1‑94 (MQ=60)
              AGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGC                                                                                 >  SRR4302545.3828452/1‑94 (MQ=60)
              AGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGC                                                                                 <  SRR4302545.647318/94‑1 (MQ=60)
               GATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCG                                                                                >  SRR4302545.6585349/1‑94 (MQ=60)
                ATCTGACCCGCGATGCGATGGGCTTCACTGAAAAACGCGGTGACTCGCTCAATGTCTTAATCTCTCCTTTCAATATCAGTGACGAAAGCGGCGG                                                                               <  SRR4302545.5636610/94‑1 (MQ=53)
                ATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGG                                                                               <  SRR4302545.10122213/94‑1 (MQ=60)
                ATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGG                                                                               >  SRR4302545.9120665/1‑94 (MQ=60)
                 TCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGA                                                                              <  SRR4302545.10166415/94‑1 (MQ=60)
                 TCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGA                                                                              <  SRR4302545.2298165/94‑1 (MQ=60)
                  CTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAG                                                                             >  SRR4302545.5005161/1‑94 (MQ=60)
                  CTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAG                                                                             >  SRR4302545.4965929/1‑94 (MQ=60)
                   TGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGA                                                                            >  SRR4302545.832264/1‑94 (MQ=60)
                   TGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGA                                                                            <  SRR4302545.1379897/94‑1 (MQ=60)
                   TGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGA                                                                            >  SRR4302545.6454978/1‑94 (MQ=60)
                    GACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAA                                                                           >  SRR4302545.6294097/1‑94 (MQ=60)
                    GACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAA                                                                           >  SRR4302545.8742691/1‑94 (MQ=60)
                     ACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAAC                                                                          >  SRR4302545.10266584/1‑94 (MQ=60)
                      CCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCTGTTCAATAGCGGTGACGAAAGCGGCGGAGAACT                                                                         <  SRR4302545.6172224/94‑1 (MQ=60)
                      CCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACT                                                                         <  SRR4302545.6172225/94‑1 (MQ=60)
                       CCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCTCTGTCGTTAACTCGCCGTTCAATCGCGGCGACGAAAGCGGCGGAGAACTG                                                                        >  SRR4302545.6003068/1‑94 (MQ=60)
                        CGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGC                                                                       >  SRR4302545.3573204/1‑94 (MQ=60)
                          CGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCA                                                                     >  SRR4302545.9067900/1‑94 (MQ=60)
                           GAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCAT                                                                    >  SRR4302545.6147667/1‑94 (MQ=60)
                           GAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCAT                                                                    >  SRR4302545.4840195/1‑94 (MQ=60)
                           GAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCAT                                                                    >  SRR4302545.5176017/1‑94 (MQ=60)
                             GGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTC                                                                  >  SRR4302545.8803096/1‑94 (MQ=60)
                              GCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCT                                                                 <  SRR4302545.9526859/94‑1 (MQ=60)
                              GCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCT                                                                 <  SRR4302545.1488008/94‑1 (MQ=60)
                              GCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCA                                                                 <  SRR4302545.1488009/94‑1 (MQ=60)
                               CGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTG                                                                <  SRR4302545.3956692/94‑1 (MQ=60)
                               CGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTG                                                                >  SRR4302545.4630499/1‑94 (MQ=60)
                                  TGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCA                                                             >  SRR4302545.9245446/1‑94 (MQ=60)
                                   GGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAA                                                            <  SRR4302545.7166514/94‑1 (MQ=60)
                                     GCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACA                                                          >  SRR4302545.8927893/1‑94 (MQ=60)
                                     GCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACA                                                          >  SRR4302545.8680100/1‑94 (MQ=60)
                                     GCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGACATTCTGGCAACA                                                          >  SRR4302545.5297330/1‑94 (MQ=60)
                                     GCTTTTCTGAAAAACGCGGTGACTCGCTAAATGTCGTTAACGCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCACCA                                                          <  SRR4302545.10664996/94‑1 (MQ=60)
                                      CTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAG                                                         >  SRR4302545.5456157/1‑94 (MQ=60)
                                          TCTGAAAAACGCGGTGACTCGCTCAATGTCGTTACCTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAG                                                     <  SRR4302545.6415235/94‑1 (MQ=60)
                                          TCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAG                                                     <  SRR4302545.5404870/94‑1 (MQ=60)
                                          TCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAG                                                     <  SRR4302545.5404869/94‑1 (MQ=60)
                                          TCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAG                                                     <  SRR4302545.6996657/94‑1 (MQ=60)
                                          TCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAG                                                     <  SRR4302545.6415236/94‑1 (MQ=60)
                                          TCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAG                                                     <  SRR4302545.5910644/94‑1 (MQ=60)
                                           CTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGC                                                    >  SRR4302545.1784472/1‑94 (MQ=60)
                                           CTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGC                                                    <  SRR4302545.1211953/94‑1 (MQ=60)
                                           CTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGC                                                    <  SRR4302545.434944/94‑1 (MQ=60)
                                             GAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGT                                                  <  SRR4302545.1302365/94‑1 (MQ=60)
                                              AAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTT                                                 >  SRR4302545.10904895/1‑94 (MQ=60)
                                                 AACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTAT                                              <  SRR4302545.7067367/94‑1 (MQ=60)
                                                 AACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTAT                                              <  SRR4302545.9946706/94‑1 (MQ=60)
                                                 AACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTAT                                              >  SRR4302545.2488234/1‑94 (MQ=60)
                                                 AACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTAT                                              >  SRR4302545.3057301/1‑94 (MQ=60)
                                                  ACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATC                                             <  SRR4302545.10454898/94‑1 (MQ=60)
                                                    GCGATGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGA                                           <  SRR4302545.5631998/94‑1 (MQ=60)
                                                       GTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCA                                        <  SRR4302545.4273936/94‑1 (MQ=60)
                                                       GTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCA                                        <  SRR4302545.2725511/94‑1 (MQ=60)
                                                          ACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTT                                     <  SRR4302545.8138633/94‑1 (MQ=60)
                                                            TCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTAC                                   <  SRR4302545.938318/94‑1 (MQ=60)
                                                            TCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTAC                                   >  SRR4302545.5398942/1‑94 (MQ=60)
                                                             CGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACT                                  >  SRR4302545.1119449/1‑94 (MQ=60)
                                                             CGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACT                                  <  SRR4302545.501992/94‑1 (MQ=60)
                                                               CTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTG                                <  SRR4302545.9174284/94‑1 (MQ=60)
                                                               CTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTG                                >  SRR4302545.1383513/1‑94 (MQ=60)
                                                                TCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAATTACTTGC                               >  SRR4302545.6822511/1‑94 (MQ=60)
                                                                 CAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCT                              <  SRR4302545.6133017/94‑1 (MQ=60)
                                                                 CAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCT                              <  SRR4302545.7911363/94‑1 (MQ=60)
                                                                  AATGTCGTTAACTCGCCGTTCAATAGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTG                             <  SRR4302545.10812044/94‑1 (MQ=60)
                                                                   ATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGC                            >  SRR4302545.371454/1‑94 (MQ=60)
                                                                   ATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGC                            <  SRR4302545.410482/94‑1 (MQ=60)
                                                                   ATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGC                            <  SRR4302545.10734248/94‑1 (MQ=60)
                                                                    TGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCC                           <  SRR4302545.10092505/94‑1 (MQ=60)
                                                                     GTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCG                          <  SRR4302545.6855344/94‑1 (MQ=60)
                                                                     GTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCG                          <  SRR4302545.8113956/94‑1 (MQ=60)
                                                                       CGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGT                        >  SRR4302545.1370411/1‑94 (MQ=60)
                                                                       CGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTACCGGT                        >  SRR4302545.1200210/1‑94 (MQ=60)
                                                                        GTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTC                       <  SRR4302545.5757539/94‑1 (MQ=60)
                                                                        GTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTC                       >  SRR4302545.4123895/1‑94 (MQ=60)
                                                                          TAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGC                     <  SRR4302545.7263057/94‑1 (MQ=60)
                                                                            ACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTG                   >  SRR4302545.9535181/1‑94 (MQ=60)
                                                                             CTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGG                  >  SRR4302545.7331498/1‑94 (MQ=60)
                                                                             CTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGG                  >  SRR4302545.7331499/1‑94 (MQ=60)
                                                                                 CCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGC              >  SRR4302545.2043964/1‑94 (MQ=60)
                                                                                  CGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCT             >  SRR4302545.1579224/1‑94 (MQ=60)
                                                                                  CGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCT             >  SRR4302545.3592458/1‑94 (MQ=60)
                                                                                   GTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATCCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTG            >  SRR4302545.7408178/1‑94 (MQ=60)
                                                                                    TTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGG           <  SRR4302545.2786489/94‑1 (MQ=60)
                                                                                     TCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGT          >  SRR4302545.8612906/1‑94 (MQ=60)
                                                                                      CAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTA         <  SRR4302545.10738966/94‑1 (MQ=60)
                                                                                      CAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTA         >  SRR4302545.4139203/1‑94 (MQ=60)
                                                                                       AATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTAC        >  SRR4302545.4221644/1‑94 (MQ=60)
                                                                                        ATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACT       >  SRR4302545.1251615/1‑94 (MQ=60)
                                                                                         TAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTG      <  SRR4302545.7211598/94‑1 (MQ=60)
                                                                                           GCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTGCT    <  SRR4302545.254891/94‑1 (MQ=60)
                                                                                           GCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTGCT    <  SRR4302545.156976/94‑1 (MQ=60)
                                                                                           GCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTGCT    >  SRR4302545.1026084/1‑94 (MQ=60)
                                                                                            CGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTGCTG   >  SRR4302545.3115628/1‑94 (MQ=60)
                                                                                            CGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTGCTG   <  SRR4302545.2966095/94‑1 (MQ=60)
                                                                                             GGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTGCTGG  <  SRR4302545.3417972/94‑1 (MQ=60)
                                                                                             GGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTGCTGG  >  SRR4302545.5684528/1‑94 (MQ=60)
                                                                                             |                                                                                             
ATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTGCTGG  >  NC_000913/2012451‑2012637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑