Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,012,544	A→G	S432G (AGT→GGT)	fliF →	FliF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,012,544	0	A	G	100.0%	685.8 / NA	196	S432G (AGT→GGT)	fliF	FliF
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (82/114); total (82/114)

Only 100 of 202 total aligned reads displayed.

AAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACT  >  NC_000913/2012454‑2012632

aaGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGt                                                                                        >  1:6326257/1‑93 (MQ=255)

 aGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTg                                                                                       >  2:493874/1‑93 (MQ=255)

   gaaaGTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGAc                                                                                     <  2:6457902/92‑1 (MQ=255)

     aaTTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGa                                                                                   <  1:6096956/93‑1 (MQ=255)

     aaTTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGa                                                                                   <  1:5558581/93‑1 (MQ=255)

      atagAAGATCTGACCCGCGAGACGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGaa                                                                                  <  1:6471847/90‑1 (MQ=255)

       ttGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATACCGGTGACGaaa                                                                                 <  1:5119741/93‑1 (MQ=255)

        tGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAg                                                                                <  2:5602117/93‑1 (MQ=255)

         gAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAgc                                                                               <  1:5769212/93‑1 (MQ=255)

         gAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAgc                                                                               <  2:2490729/93‑1 (MQ=255)

         gAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAgc                                                                               <  1:3431374/93‑1 (MQ=255)

          aaGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAgcg                                                                              <  1:1921270/93‑1 (MQ=255)

            gATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAgcggc                                                                            <  2:3973421/93‑1 (MQ=255)

             aTCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAgcggcg                                                                           >  2:1296699/1‑93 (MQ=255)

              tCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGcggcgg                                                                          <  2:6248633/93‑1 (MQ=255)

              tCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGcggcgg                                                                          >  1:413114/1‑93 (MQ=255)

               cTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTACCTCGCCGTTCAATAGCGGTGACGAAAGCGGCGga                                                                         <  1:2359648/93‑1 (MQ=255)

                 gACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGgaga                                                                       >  1:2237834/1‑93 (MQ=255)

                 gACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGgaga                                                                       >  1:4228969/1‑93 (MQ=255)

                  aCCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGaa                                                                      <  1:1984551/93‑1 (MQ=255)

                   cccGCGAGGCGATGGGCTTTTCTGAAAAACGGGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAAc                                                                     <  1:3132869/93‑1 (MQ=255)

                   cccGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAAc                                                                     <  1:5363593/93‑1 (MQ=255)

                   cccGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAAc                                                                     <  2:1856364/93‑1 (MQ=255)

                      gcgAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTGACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGc                                                                  >  2:446608/1‑93 (MQ=255)

                       cgAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGcc                                                                 <  1:256941/93‑1 (MQ=255)

                        gAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCa                                                                >  2:5861795/1‑93 (MQ=255)

                        gAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCa                                                                <  1:381259/93‑1 (MQ=255)

                         aGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCAt                                                               <  1:1378090/93‑1 (MQ=255)

                           gCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCAttg                                                             >  2:5347962/1‑92 (MQ=255)

                            cGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACTAAAGCGGCGGAGAACTGCCATTCt                                                            <  2:3267280/93‑1 (MQ=255)

                            cGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCt                                                            <  1:437793/93‑1 (MQ=255)

                             gATTGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTg                                                           >  1:6371276/1‑93 (MQ=255)

                               tGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGc                                                         <  1:2348176/93‑1 (MQ=255)

                                gggCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCa                                                        >  1:1532572/1‑93 (MQ=255)

                                 ggCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCaa                                                       >  1:2189664/1‑93 (MQ=255)

                                  gCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAAc                                                      <  2:3157941/93‑1 (MQ=255)

                                  gCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAAc                                                      >  2:5400023/1‑93 (MQ=255)

                                   cTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAACCGGCGGAGAACTGCCATTCTGGCAACa                                                     >  1:4734567/1‑93 (MQ=255)

                                    ttttCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAg                                                    >  2:4137253/1‑93 (MQ=255)

                                    ttttCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAg                                                    >  1:2157646/1‑93 (MQ=255)

                                    ttttCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGCGACGAAAGCTGCGGAGAACTGCCATTCTGGCAACAg                                                    >  2:590150/1‑93 (MQ=255)

                                     tttCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGc                                                   <  1:131806/93‑1 (MQ=255)

                                     tttCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGc                                                   >  1:5918909/1‑93 (MQ=255)

                                       tCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCaa                                                 <  2:4493236/93‑1 (MQ=255)

                                       tCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCaa                                                 >  2:1704375/1‑93 (MQ=255)

                                       tCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCaa                                                 <  2:1054557/93‑1 (MQ=255)

                                       tCTAAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCaa                                                 <  2:258979/93‑1 (MQ=255)

                                        cTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAg                                                <  1:1069357/93‑1 (MQ=255)

                                        cTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAg                                                <  2:187390/93‑1 (MQ=255)

                                         tGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGc                                               <  1:2829627/93‑1 (MQ=255)

                                         tGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGc                                               >  1:339477/1‑93 (MQ=255)

                                            aaaaCGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGtt                                            <  2:2810622/93‑1 (MQ=255)

                                            aaaaCGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGtt                                            <  1:654206/93‑1 (MQ=255)

                                             aaaCGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGttt                                           >  1:1613468/1‑93 (MQ=255)

                                              aaCGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTa                                          <  1:2293696/93‑1 (MQ=255)

                                              aaCGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTa                                          <  1:480404/93‑1 (MQ=255)

                                               aCGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTAt                                         <  2:5072855/93‑1 (MQ=255)

                                               aCGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTAt                                         <  1:1792011/93‑1 (MQ=255)

                                                cgcgGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATc                                        <  2:3377279/93‑1 (MQ=255)

                                                  cgGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGa                                      >  1:1770748/1‑93 (MQ=255)

                                                  cgGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGa                                      >  2:3753625/1‑93 (MQ=255)

                                                  cgGTGACACGCTAAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGa                                      <  1:6180913/93‑1 (MQ=255)

                                                   ggTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGAt                                     >  2:4678430/1‑93 (MQ=255)

                                                    gTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATc                                    >  2:26125/1‑93 (MQ=255)

                                                    gTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATc                                    >  1:2951925/1‑93 (MQ=255)

                                                     tGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCa                                   <  1:4077920/93‑1 (MQ=255)

                                                     tGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCa                                   <  1:5895658/93‑1 (MQ=255)

                                                      gACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAg                                  <  1:523741/93‑1 (MQ=255)

                                                      gACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCCATCAg                                  >  1:1435122/1‑93 (MQ=255)

                                                        cTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGtt                                <  2:2862475/93‑1 (MQ=255)

                                                        cTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGtt                                >  2:1200020/1‑93 (MQ=255)

                                                         tCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTa                               <  2:5761458/93‑1 (MQ=255)

                                                         ccGCTAAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTa                               <  2:2369914/92‑1 (MQ=255)

                                                           gCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACt                             >  1:941983/1‑93 (MQ=255)

                                                           gCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACt                             <  2:4785967/93‑1 (MQ=255)

                                                           gCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACt                             <  2:1912383/93‑1 (MQ=255)

                                                            ctaaaTGTCGTTAACTCGCCGTTCTATAGAGGAGAAGAACGCGGCGGAGACCTGCCATTCTGGCAACAGCAACAATTTATTGCTCAGGTACtt                            >  2:593531/4‑93 (MQ=255)

                                                            cTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACtt                            <  1:5660253/93‑1 (MQ=255)

                                                            cTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACtt                            <  2:1619080/93‑1 (MQ=255)

                                                             tCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTtg                           <  1:4998680/93‑1 (MQ=255)

                                                              cAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTtgc                          <  2:4318651/93‑1 (MQ=255)

                                                               aaTGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTtgct                         >  2:5499819/1‑93 (MQ=255)

                                                                aTGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTtgctg                        >  2:3015323/1‑93 (MQ=255)

                                                                     gTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGt                   >  2:2031702/1‑93 (MQ=255)

                                                                      ttAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTc                  <  2:2757149/93‑1 (MQ=255)

                                                                      ttAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTc                  >  2:3204886/1‑93 (MQ=255)

                                                                            cGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGt            >  2:4620960/1‑93 (MQ=255)

                                                                                gTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCt        <  2:1801647/93‑1 (MQ=255)

                                                                                gTTCAATAGCGGTGACGAAAGCAGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCt        >  2:3498934/1‑93 (MQ=255)

                                                                                 ttCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTg       <  1:2694209/93‑1 (MQ=255)

                                                                                 ttCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTg       <  1:4692873/93‑1 (MQ=255)

                                                                                  tCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTgg      >  2:2280357/1‑93 (MQ=255)

                                                                                  tCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTgg      >  1:142535/1‑93 (MQ=255)

                                                                                    aaTAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTa    <  1:967747/93‑1 (MQ=255)

                                                                                     aTAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTAc   >  1:3533255/1‑93 (MQ=255)

                                                                                     aTAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTAc   <  2:4700961/93‑1 (MQ=255)

                                                                                     aTAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTAc   <  2:4067600/93‑1 (MQ=255)

                                                                                     aTAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTAc   <  2:141917/93‑1 (MQ=255)

                                                                                     aTAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTAc   >  1:5991710/1‑93 (MQ=255)

                                                                                     aTAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCATTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTAc   >  1:3700751/1‑93 (MQ=255)

AAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACT  >  NC_000913/2012454‑2012632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTGCTGG  >  NC_000913/2012451‑2012637

ATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCG                                                                                               <  SRR4302537.1297925/94‑1 (MQ=60)

ATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCG                                                                                               >  SRR4302537.1961832/1‑94 (MQ=60)

 TGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGG                                                                                              >  SRR4302537.5474254/1‑94 (MQ=60)

 TGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGG                                                                                              >  SRR4302537.1291495/1‑94 (MQ=60)

 TGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGG                                                                                              <  SRR4302537.3792004/94‑1 (MQ=60)

 TGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGG                                                                                              <  SRR4302537.1686341/94‑1 (MQ=60)

 TGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGG                                                                                              >  SRR4302537.5673739/1‑94 (MQ=60)

 TGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGG                                                                                              <  SRR4302537.1456751/94‑1 (MQ=60)

  GAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGT                                                                                             <  SRR4302537.1237115/94‑1 (MQ=60)

  GAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGT                                                                                             >  SRR4302537.6383732/1‑94 (MQ=60)

  GAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGT                                                                                             >  SRR4302537.6369526/1‑94 (MQ=60)

  GAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGT                                                                                             <  SRR4302537.2323026/94‑1 (MQ=60)

     GCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGAC                                                                                          >  SRR4302537.2196274/1‑94 (MQ=60)

        AATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAA                                                                                       <  SRR4302537.6138848/94‑1 (MQ=60)

        AATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAA                                                                                       <  SRR4302537.5596562/94‑1 (MQ=60)

         ATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAA                                                                                      >  SRR4302537.984432/1‑94 (MQ=60)

         ATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAA                                                                                      >  SRR4302537.142924/1‑94 (MQ=60)

         ATAGAAGATCTGACCCGCGAGACGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAA                                                                                      <  SRR4302537.6515738/94‑1 (MQ=60)

          TTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATACCGGTGACGAAAG                                                                                     <  SRR4302537.5155026/94‑1 (MQ=60)

            GAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCG                                                                                   <  SRR4302537.3455662/94‑1 (MQ=60)

            GAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCG                                                                                   <  SRR4302537.5808755/94‑1 (MQ=60)

             AAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGG                                                                                  <  SRR4302537.1934614/94‑1 (MQ=60)

             AAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGG                                                                                  <  SRR4302537.5132472/94‑1 (MQ=60)

               GATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCG                                                                                >  SRR4302537.4771928/1‑94 (MQ=60)

                ATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACTCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGG                                                                               <  SRR4302537.474605/94‑1 (MQ=60)

                ATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGG                                                                               >  SRR4302537.415973/1‑94 (MQ=60)

                ATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGAGACGAAAGCGGCGG                                                                               >  SRR4302537.5717783/1‑94 (MQ=60)

                  CTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTACCTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAG                                                                             <  SRR4302537.2376290/94‑1 (MQ=60)

                  CTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAG                                                                             >  SRR4302537.1745299/1‑94 (MQ=60)

                  CTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAG                                                                             >  SRR4302537.2905198/1‑94 (MQ=60)

                   TGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGA                                                                            >  SRR4302537.4258413/1‑94 (MQ=60)

                   TGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGA                                                                            >  SRR4302537.2253616/1‑94 (MQ=60)

                     ACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAAC                                                                          <  SRR4302537.1998341/94‑1 (MQ=60)

                     ACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAAC                                                                          <  SRR4302537.878050/94‑1 (MQ=60)

                     ACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAAC                                                                          <  SRR4302537.5634162/94‑1 (MQ=60)

                     ACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAAC                                                                          <  SRR4302537.1378239/94‑1 (MQ=60)

                      CCCGCGAGGCGATGGGCTTTTCTGAAAAACGGGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACT                                                                         <  SRR4302537.3154854/94‑1 (MQ=60)

                      CCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACT                                                                         <  SRR4302537.5400305/94‑1 (MQ=60)

                      CCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACT                                                                         >  SRR4302537.143569/1‑94 (MQ=60)

                          CGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCA                                                                     <  SRR4302537.258762/94‑1 (MQ=60)

                          CGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCA                                                                     >  SRR4302537.3022831/1‑94 (MQ=60)

                           GAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCAT                                                                    <  SRR4302537.2365496/94‑1 (MQ=60)

                           GAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCAT                                                                    >  SRR4302537.6211826/1‑94 (MQ=60)

                           GAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCAT                                                                    <  SRR4302537.383903/94‑1 (MQ=60)

                            AGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATT                                                                   <  SRR4302537.1387711/94‑1 (MQ=60)

                             GGCGATGGTCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGTCGGAGAACTGCCATTC                                                                  <  SRR4302537.1491703/94‑1 (MQ=60)

                             GGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTC                                                                  >  SRR4302537.6462437/1‑94 (MQ=60)

                              GCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCT                                                                 <  SRR4302537.1794726/94‑1 (MQ=60)

                               CGATTGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTG                                                                >  SRR4302537.6414727/1‑94 (MQ=60)

                               CGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTG                                                                <  SRR4302537.440843/94‑1 (MQ=60)

                                GATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGG                                                               <  SRR4302537.4906916/94‑1 (MQ=60)

                                  TGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCA                                                             <  SRR4302537.2364710/94‑1 (MQ=60)

                                  TGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCA                                                             >  SRR4302537.1543282/1‑94 (MQ=60)

                                   GGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAA                                                            >  SRR4302537.2205089/1‑94 (MQ=60)

                                   GGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAA                                                            >  SRR4302537.2747510/1‑94 (MQ=60)

                                     GCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACA                                                          <  SRR4302537.3421105/94‑1 (MQ=60)

                                     GCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACA                                                          <  SRR4302537.3821208/94‑1 (MQ=60)

                                     GCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAACCGGCGGAGAACTGCCATTCTGGCAACA                                                          >  SRR4302537.4767282/1‑94 (MQ=60)

                                      GTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGTTGCCGAAAGCGGCGGAGAACTGCCATTCTGGCAACAG                                                         <  SRR4302537.2443555/94‑1 (MQ=60)

                                      CTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAG                                                         >  SRR4302537.2172851/1‑94 (MQ=60)

                                      CTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAG                                                         >  SRR4302537.3678976/1‑94 (MQ=60)

                                      CTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAG                                                         <  SRR4302537.652637/94‑1 (MQ=60)

                                       TTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGC                                                        >  SRR4302537.5528000/1‑94 (MQ=60)

                                       TTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGC                                                        >  SRR4302537.5959526/1‑94 (MQ=60)

                                        TTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCA                                                       <  SRR4302537.132741/94‑1 (MQ=60)

                                           CTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGC                                                    >  SRR4302537.341831/1‑94 (MQ=60)

                                           CTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGC                                                    <  SRR4302537.1076728/94‑1 (MQ=60)

                                            TGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACCGCAAGCG                                                   >  SRR4302537.3877597/1‑94 (MQ=60)

                                            TGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCG                                                   <  SRR4302537.2849396/94‑1 (MQ=60)

                                            TGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCG                                                   <  SRR4302537.5828538/94‑1 (MQ=60)

                                               AAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTT                                                >  SRR4302537.1624744/1‑94 (MQ=60)

                                               AAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTT                                                <  SRR4302537.658662/94‑1 (MQ=60)

                                                 AACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTAT                                              <  SRR4302537.2309887/94‑1 (MQ=60)

                                                 AACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTAT                                              <  SRR4302537.483697/94‑1 (MQ=60)

                                                  ACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATC                                             <  SRR4302537.1804521/94‑1 (MQ=60)

                                                    GCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGA                                           >  SRR4302537.1783079/1‑94 (MQ=60)

                                                     CGGTGACACGCTAAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGAT                                          <  SRR4302537.6223489/94‑1 (MQ=60)

                                                      GGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATC                                         >  SRR4302537.2972642/1‑94 (MQ=60)

                                                        TGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAG                                       <  SRR4302537.4106391/94‑1 (MQ=60)

                                                        TGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAG                                       >  SRR4302537.1991203/1‑94 (MQ=60)

                                                        TGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAG                                       <  SRR4302537.5936109/94‑1 (MQ=60)

                                                        TGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCCATCAG                                       >  SRR4302537.1445102/1‑94 (MQ=60)

                                                         GACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGT                                      <  SRR4302537.527335/94‑1 (MQ=60)

                                                          ACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTT                                     <  SRR4302537.1226421/94‑1 (MQ=60)

                                                           CTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTA                                    <  SRR4302537.1562724/94‑1 (MQ=60)

                                                            TCGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTAC                                   <  SRR4302537.2327750/94‑1 (MQ=60)

                                                             CGCTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACT                                  >  SRR4302537.948480/1‑94 (MQ=60)

                                                               CTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTG                                <  SRR4302537.5267214/94‑1 (MQ=60)

                                                               CTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTG                                <  SRR4302537.2244346/94‑1 (MQ=60)

                                                               CTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTG                                <  SRR4302537.5698990/94‑1 (MQ=60)

                                                               CTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTG                                >  SRR4302537.4724485/1‑94 (MQ=60)

                                                               CTCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTATATCGATCAGTTACTTG                                >  SRR4302537.5963355/1‑94 (MQ=60)

                                                                TCAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGC                               <  SRR4302537.5033125/94‑1 (MQ=60)

                                                                 CAATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCT                              <  SRR4302537.2111416/94‑1 (MQ=60)

                                                                  AATGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTG                             >  SRR4302537.4206012/1‑94 (MQ=60)

                                                                    TGTCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCC                           >  SRR4302537.1896383/1‑94 (MQ=60)

                                                                      TCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGG                         <  SRR4302537.878578/94‑1 (MQ=60)

                                                                      TCGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGG                         >  SRR4302537.6343010/1‑94 (MQ=60)

                                                                       CGTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGT                        <  SRR4302537.6115419/94‑1 (MQ=60)

                                                                        GTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTC                       <  SRR4302537.3455718/94‑1 (MQ=60)

                                                                        GTTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTC                       >  SRR4302537.2291790/1‑94 (MQ=60)

                                                                         TTAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCG                      <  SRR4302537.5942237/94‑1 (MQ=60)

                                                                          TAACTCGCCGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGC                     >  SRR4302537.966582/1‑94 (MQ=60)

                                                                                  CGTTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCT             <  SRR4302537.4126456/94‑1 (MQ=60)

                                                                                    TTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGG           >  SRR4302537.143548/1‑94 (MQ=60)

                                                                                    TTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGG           <  SRR4302537.2713035/94‑1 (MQ=60)

                                                                                    TTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGG           <  SRR4302537.3407451/94‑1 (MQ=60)

                                                                                    TTCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGG           <  SRR4302537.4725333/94‑1 (MQ=60)

                                                                                     TCAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGT          <  SRR4302537.5310006/94‑1 (MQ=60)

                                                                                      CAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTA         >  SRR4302537.2669600/1‑94 (MQ=60)

                                                                                      CAATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTA         >  SRR4302537.4311855/1‑94 (MQ=60)

                                                                                       AATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTAC        >  SRR4302537.6032860/1‑94 (MQ=60)

                                                                                       AATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTAC        >  SRR4302537.3558222/1‑94 (MQ=60)

                                                                                       AATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTAC        <  SRR4302537.974418/94‑1 (MQ=60)

                                                                                       AATAGCGGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCATTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTAC        >  SRR4302537.3726784/1‑94 (MQ=60)

                                                                                             GGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTGCTGG  <  SRR4302537.6256131/94‑1 (MQ=60)

                                                                                             GGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTGCTGG  <  SRR4302537.2990852/94‑1 (MQ=60)

ATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTGCTGG  >  NC_000913/2012451‑2012637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`