Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	647	270.1	21258240	98.4%	20918108	94.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,082,410	T→C	A22A (GCA→GCG)	yeeD ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,082,410	0	T	C	98.2%	762.9 / ‑2.0	226	A22A (GCA→GCG)	yeeD	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (3/1); new base C (102/120); total (105/121)
Fisher's exact test for biased strand distribution p-value = 3.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 240 total aligned reads displayed.

CTTCTGCCGCCCACTGTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCTGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACCTTACTTAATTTGCCGC  >  NC_000913/2082319‑2082500

cttctGCCGCCCACTGTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAgccgc                                                                                          <  1:7868742/94‑1 (MQ=255)

cttctGCCGCCCACTGTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAgccgc                                                                                          >  1:4528475/1‑94 (MQ=255)

 ttctGCCGCCCACTGTGGGATTGCTTCAGTTGTCTGGGGGCAATCAAACTCAATCTCTAACTCATCGCCGCTGACCATCTCTGCCTGAgccgct                                                                                         >  1:8041523/1‑94 (MQ=255)

  tctGCCGCCCACTGTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCtt                                                                                        <  1:5617688/94‑1 (MQ=255)

   ctGCCGCCCACTGTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCttt                                                                                       <  1:2896925/94‑1 (MQ=255)

    tGCCGCCCACTGTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCtttt                                                                                      >  2:2252336/1‑94 (MQ=255)

     gccgccCACTGTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTg                                                                                     <  1:9200828/94‑1 (MQ=255)

     gccgccCACTGTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTg                                                                                     >  2:10173409/1‑94 (MQ=255)

      ccgccCACTGTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGc                                                                                    <  1:8824035/94‑1 (MQ=255)

      ccgccCACTGTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGc                                                                                    >  1:317065/1‑94 (MQ=255)

      ccgccCACTGTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGc                                                                                    <  1:4709510/94‑1 (MQ=255)

        gccCACTGTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCtt                                                                                  >  1:1346700/1‑94 (MQ=255)

         cccACTGTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTc                                                                                 >  2:7723300/1‑94 (MQ=255)

         cccACTGTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTc                                                                                 <  1:6096682/94‑1 (MQ=255)

          ccACTGTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAATTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCt                                                                                <  1:4789754/94‑1 (MQ=255)

           cACTGTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTa                                                                               >  1:5064780/1‑94 (MQ=255)

             cTGTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATg                                                                             <  1:4072904/94‑1 (MQ=255)

              tgtgGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGa                                                                            <  2:435856/94‑1 (MQ=255)

              tgtgGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGa                                                                            <  2:5272035/94‑1 (MQ=255)

              tgtgGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGa                                                                            >  2:1743481/1‑94 (MQ=255)

               gtgtGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACGAACTCACCGCCGCTGACCATGGCTGCCAGAGCTGCTTTTGCTTCTATGAg                                                                           >  1:9370319/1‑94 (MQ=255)

               gtgGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAg                                                                           >  1:6391602/1‑94 (MQ=255)

                 gggATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTg                                                                         <  1:5973317/94‑1 (MQ=255)

                  ggATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTgg                                                                        >  1:7754008/1‑94 (MQ=255)

                  ggATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTgg                                                                        <  1:5720202/94‑1 (MQ=255)

                    aTTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGaa                                                                      >  2:1750710/1‑94 (MQ=255)

                     ttGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGaaa                                                                     <  2:2772799/94‑1 (MQ=255)

                     ttGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGaaa                                                                     >  2:4028637/1‑94 (MQ=255)

                       gCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACg                                                                   >  1:2315442/1‑94 (MQ=255)

                       gCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCGGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACg                                                                   <  1:1542146/94‑1 (MQ=255)

                          tCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGAc                                                                <  1:10135605/94‑1 (MQ=255)

                          tCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTACCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGAc                                                                <  2:6058747/94‑1 (MQ=255)

                             gTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGa                                                             >  2:5815131/1‑94 (MQ=255)

                                gCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCt                                                          <  2:9651240/94‑1 (MQ=255)

                                gCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCt                                                          <  2:3846201/94‑1 (MQ=255)

                                gCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCt                                                          <  2:9808625/94‑1 (MQ=255)

                                 ccTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTg                                                         >  1:5768488/1‑94 (MQ=255)

                                   tGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCg                                                       >  2:9264226/1‑94 (MQ=255)

                                   tGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCg                                                       >  2:1054271/1‑94 (MQ=255)

                                   tGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTAGGAGTGGAAACGGACCGACGTGCg                                                       >  2:1356228/1‑94 (MQ=255)

                                      gTGCAATCAAACTCAATCAATACCTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTca                                                    <  1:5865509/94‑1 (MQ=255)

                                       tGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTcac                                                   <  2:8804107/94‑1 (MQ=255)

                                          aaTCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTcaccac                                                <  1:4089289/94‑1 (MQ=255)

                                              aaaCTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATcc                                            <  2:2899263/94‑1 (MQ=255)

                                              aaaCTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATcc                                            >  1:8658587/1‑94 (MQ=255)

                                              aaaCTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATcc                                            >  2:371267/1‑94 (MQ=255)

                                              aaaCTCAATAACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATcc                                            <  1:7100361/94‑1 (MQ=255)

                                               aaCTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCa                                           <  1:6310991/94‑1 (MQ=255)

                                               aaCTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCa                                           <  2:9064131/94‑1 (MQ=255)

                                               aaCTCAAGCACTAACTCATCGCCGCTGACCATGTCTGCCAGAGCCGCGTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCa                                           <  2:5516090/94‑1 (MQ=255)

                                                aCTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAg                                          <  1:5759775/94‑1 (MQ=255)

                                                aCTCAATCACTAACTCATCGCCGCTGACCATCTCTGCCATTGCGGCTTTTGCGTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAg                                          <  2:8973917/94‑1 (MQ=255)

                                                 cccAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGc                                         <  2:2011708/92‑1 (MQ=255)

                                                 cTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGc                                         <  2:4725513/94‑1 (MQ=255)

                                                   cAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCtt                                       >  1:2084917/1‑94 (MQ=255)

                                                     aTCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCtttt                                     <  1:10390746/94‑1 (MQ=255)

                                                       cACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTa                                   <  2:5407405/94‑1 (MQ=255)

                                                       cACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTa                                   >  2:8530342/1‑94 (MQ=255)

                                                       cACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTa                                   >  2:3389997/1‑94 (MQ=255)

                                                       cACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTa                                   <  1:8415626/94‑1 (MQ=255)

                                                        aCTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTaa                                  <  1:7572494/94‑1 (MQ=255)

                                                          tAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATc                                >  1:343644/1‑94 (MQ=255)

                                                          tAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATc                                <  2:5539822/94‑1 (MQ=255)

                                                           aaCTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCa                               >  2:5570920/1‑94 (MQ=255)

                                                           aaCTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCa                               <  2:7248611/94‑1 (MQ=255)

                                                            aCTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCAc                              >  1:1620054/1‑94 (MQ=255)

                                                                aTCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATc                          <  2:507569/94‑1 (MQ=255)

                                                                  cgccgcTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCtt                        <  2:590023/94‑1 (MQ=255)

                                                                   gccgcTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCttt                       <  1:8736312/94‑1 (MQ=255)

                                                                   gccgcTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCttt                       >  2:8243415/1‑94 (MQ=255)

                                                                    ccgcTGACCATTTCTGCCAGAGCTGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTa                      >  1:5296356/1‑94 (MQ=255)

                                                                    ccgcTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTa                      <  1:10238355/94‑1 (MQ=255)

                                                                    ccgcTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTa                      >  2:431039/1‑94 (MQ=255)

                                                                    ccgcTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTa                      <  1:2632647/94‑1 (MQ=255)

                                                                     cgcTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTac                     <  2:1084829/94‑1 (MQ=255)

                                                                       cTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTacac                   >  2:7696654/1‑94 (MQ=255)

                                                                        tGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACAcc                  >  1:1794101/1‑94 (MQ=255)

                                                                        tGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACAcc                  <  1:3929006/94‑1 (MQ=255)

                                                                        tGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACAcc                  >  1:6911907/1‑94 (MQ=255)

                                                                        tGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACAcc                  <  1:2464700/94‑1 (MQ=255)

                                                                        tGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACAcc                  >  2:6374563/1‑94 (MQ=255)

                                                                          gccATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACctt                <  1:9682972/93‑1 (MQ=255)

                                                                          aCCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACctt                <  1:6697461/94‑1 (MQ=255)

                                                                            cATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACcttac              >  1:8169172/1‑94 (MQ=255)

                                                                             aTTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACcttcct             >  2:5646149/1‑94 (MQ=255)

                                                                             aTTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACcttact             <  2:10431979/94‑1 (MQ=255)

                                                                              tttCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACcttactt            <  1:8912613/94‑1 (MQ=255)

                                                                               ttCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACcttactta           <  1:9827058/94‑1 (MQ=255)

                                                                               ttCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACcttactta           <  2:9840919/94‑1 (MQ=255)

                                                                               ttCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACcttactta           <  1:4986032/94‑1 (MQ=255)

                                                                                tCTGCCAGAGCCGCTTTTGCTTCTATGAGTGAAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACCTTACTTaa          >  2:1618359/1‑94 (MQ=255)

                                                                                 cTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACCTTACTTAAt         >  1:4401538/1‑94 (MQ=255)

                                                                                  tGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACCTTACTTAAtt        <  1:10618910/94‑1 (MQ=255)

                                                                                   gCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACCTTACTTAAttt       <  1:4302175/94‑1 (MQ=255)

                                                                                   gCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACCTTACTTAAttt       <  1:6727354/94‑1 (MQ=255)

                                                                                   gCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACCTTACTTAAttt       <  2:1396288/94‑1 (MQ=255)

                                                                                     cAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACCTTACTTAATTTGc     >  2:6856038/1‑94 (MQ=255)

                                                                                      agagCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACCTTACTTAATTTGcc    <  2:6093636/94‑1 (MQ=255)

                                                                                       gagCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACCTTACTTAATTTGCCg   >  2:8149848/1‑94 (MQ=255)

                                                                                        agCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACCTTACTTAATTTGCCgc  >  1:900085/1‑94 (MQ=255)

CTTCTGCCGCCCACTGTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCTGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACCTTACTTAATTTGCCGC  >  NC_000913/2082319‑2082500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TCTTCTGCCGCCCACTGTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCTGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACCTTACTTAATTTGCCGCAG  >  NC_000913/2082318‑2082502

TCTTCTGCCGCCCACTGTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCG                                                                                             <  SRR4302545.5648746/94‑1 (MQ=60)

TCTTCTGCCGCCCACTGTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCG                                                                                             <  SRR4302545.1529991/94‑1 (MQ=60)

TCTTCTGCCGCCCACTGTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCG                                                                                             <  SRR4302545.9597053/94‑1 (MQ=60)

TCTTCTGCCGCCCACTGTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCG                                                                                             <  SRR4302545.7747533/94‑1 (MQ=60)

 CTTCTGCCGCCCACTGTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGC                                                                                            >  SRR4302545.4644510/1‑94 (MQ=60)

 CTTCTGCCGCCCACTGTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGC                                                                                            <  SRR4302545.8091975/94‑1 (MQ=60)

 CTTCTGCCGCCCACTGTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGC                                                                                            >  SRR4302545.10216077/1‑94 (MQ=60)

  TTCTGCCGCCCACTGTGGGATTGCTTCAGTTGTCTGGGGGCAATCAAACTCAATCTCTAACTCATCGCCGCTGACCATCTCTGCCTGAGCCGCT                                                                                           >  SRR4302545.8270942/1‑94 (MQ=60)

   TCTGCCGCCCACTGTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTT                                                                                          <  SRR4302545.5766907/94‑1 (MQ=60)

    CTGCCGCCCACTGTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTT                                                                                         <  SRR4302545.2967136/94‑1 (MQ=60)

      GCCGCCCACTGTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTG                                                                                       <  SRR4302545.9470527/94‑1 (MQ=60)

       CCGCCCACTGTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGC                                                                                      <  SRR4302545.9080255/94‑1 (MQ=60)

       CCGCCCACTGTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGC                                                                                      >  SRR4302545.323782/1‑94 (MQ=60)

       CCGCCCACTGTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGC                                                                                      <  SRR4302545.4829530/94‑1 (MQ=60)

         GCCCACTGTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTT                                                                                    >  SRR4302545.8306921/1‑94 (MQ=60)

         GCCCACTGTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTT                                                                                    >  SRR4302545.1376766/1‑94 (MQ=60)

          CCCACTGTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTC                                                                                   <  SRR4302545.6262345/94‑1 (MQ=60)

           CCACTGTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAATTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCT                                                                                  <  SRR4302545.4912222/94‑1 (MQ=60)

            CACTGTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTA                                                                                 >  SRR4302545.6454984/1‑94 (MQ=60)

            CACTGTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTA                                                                                 >  SRR4302545.5193728/1‑94 (MQ=60)

            CACTGTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTA                                                                                 <  SRR4302545.2139903/94‑1 (MQ=60)

            CACTCTGGGATTGCTTCAGTTGCCTGGGTGCAACAAAACTCAATCACTACCTCATCGCCGCTGACCATTCCTGCCAGAGCCGCTTTTGCTTCTT                                                                                 >  SRR4302545.5193723/1‑94 (MQ=60)

              CTGTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATG                                                                               <  SRR4302545.4174885/94‑1 (MQ=60)

              CTGTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATG                                                                               >  SRR4302545.2096182/1‑94 (MQ=60)

              CTGTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATG                                                                               >  SRR4302545.8135333/1‑94 (MQ=60)

               TGTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGA                                                                              <  SRR4302545.9887090/94‑1 (MQ=60)

                GTGTGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACGAACTCACCGCCGCTGACCATGGCTGCCAGAGCTGCTTTTGCTTCTATGAG                                                                             >  SRR4302545.9645239/1‑94 (MQ=60)

                GTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAG                                                                             >  SRR4302545.508678/1‑94 (MQ=60)

                GTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAG                                                                             >  SRR4302545.7798829/1‑94 (MQ=60)

                GTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAG                                                                             >  SRR4302545.6566375/1‑94 (MQ=60)

                 atggaaagcgccgggtgcgaggcaacaagtacACTCAATCACTAACCAATCGCCGCTGACCATTTCTACCAGAGCCGCTTTTGCcgctgtgagt                                                                            <  SRR4302545.8877075/62‑11 (MQ=33)

                  GGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTG                                                                           <  SRR4302545.6134464/94‑1 (MQ=60)

                   GGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGG                                                                          <  SRR4302545.5873626/94‑1 (MQ=60)

                   GGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGG                                                                          >  SRR4302545.7973447/1‑94 (MQ=60)

                   GGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGG                                                                          <  SRR4302545.5873627/94‑1 (MQ=60)

                      TTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAA                                                                       <  SRR4302545.9276516/94‑1 (MQ=60)

                        GCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACG                                                                     <  SRR4302545.1578631/94‑1 (MQ=60)

                        GCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACG                                                                     >  SRR4302545.2371847/1‑94 (MQ=60)

                        GCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCGGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACG                                                                     <  SRR4302545.1578330/94‑1 (MQ=60)

                          TTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGA                                                                   <  SRR4302545.4265709/94‑1 (MQ=60)

                          TTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGA                                                                   >  SRR4302545.10080617/1‑94 (MQ=60)

                           TCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGAC                                                                  <  SRR4302545.10435343/94‑1 (MQ=60)

                           TCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGAC                                                                  <  SRR4302545.3619500/94‑1 (MQ=60)

                             AGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAG                                                                <  SRR4302545.7277727/94‑1 (MQ=60)

                               TTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGAC                                                              <  SRR4302545.7983637/94‑1 (MQ=60)

                               TTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGAC                                                              <  SRR4302545.10455114/94‑1 (MQ=60)

                               TTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGAC                                                              <  SRR4302545.7121612/94‑1 (MQ=60)

                                 GCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCT                                                            <  SRR4302545.4505996/94‑1 (MQ=60)

                                 GCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCT                                                            <  SRR4302545.5338095/94‑1 (MQ=60)

                                 GCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCT                                                            <  SRR4302545.4637290/94‑1 (MQ=60)

                                  CCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTG                                                           <  SRR4302545.1741114/94‑1 (MQ=60)

                                  CCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTG                                                           >  SRR4302545.5923497/1‑94 (MQ=60)

                                  CCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCCATGAGTGGAAACGGACAGACCTG                                                           >  SRR4302545.5923496/1‑94 (MQ=60)

                                   CTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGC                                                          >  SRR4302545.8309781/1‑94 (MQ=60)

                                    TGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCG                                                         >  SRR4302545.6577054/1‑94 (MQ=60)

                                    TGGGGGCAATCAAACCCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCG                                                         <  SRR4302545.8682772/94‑1 (MQ=60)

                                     GGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGT                                                        >  SRR4302545.3328331/1‑94 (MQ=60)

                                       GTGCAATCAAGCTCACTCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCA                                                      <  SRR4302545.9643491/94‑1 (MQ=60)

                                       GTGCAATCAAACTCAATCAATACCTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCA                                                      <  SRR4302545.6023533/94‑1 (MQ=60)

                                         GCAATCAAACTCAATCACTACCTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACC                                                    <  SRR4302545.1465809/94‑1 (MQ=60)

                                         GCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACC                                                    >  SRR4302545.2427958/1‑94 (MQ=60)

                                         GCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACC                                                    >  SRR4302545.2537868/1‑94 (MQ=60)

                                         GCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACC                                                    <  SRR4302545.1465815/94‑1 (MQ=60)

                                          CAATCAAACTCAATCACTCACTCATCGGCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTGACCA                                                   >  SRR4302545.4878147/1‑94 (MQ=60)

                                          CAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCA                                                   >  SRR4302545.9115773/1‑94 (MQ=60)

                                           AATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCAC                                                  <  SRR4302545.4191684/94‑1 (MQ=60)

                                            ATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACA                                                 <  SRR4302545.591161/94‑1 (MQ=60)

                                             TCCAACTCAATCACTAACTCATCGCCGCTGTCCATTTCTCCCAGAGACGCTCTTGCTTCTATGAGTGGAAACTGACAGACatgagcaaccacat                                                >  SRR4302545.2200851/1‑80 (MQ=56)

                                              taaactacATCACTAACTCATCTGCGCTGACCAATACTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATC                                               <  SRR4302545.9071143/86‑1 (MQ=60)

                                              CAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATC                                               <  SRR4302545.3066272/94‑1 (MQ=60)

                                               AAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCC                                              >  SRR4302545.8909757/1‑94 (MQ=60)

                                               AAACTCAATAACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCC                                              <  SRR4302545.7297032/94‑1 (MQ=60)

                                                AACTCAATCTCTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCA                                             <  SRR4302545.4262471/94‑1 (MQ=60)

                                                AACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCA                                             >  SRR4302545.8668707/1‑94 (MQ=60)

                                                AACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCA                                             <  SRR4302545.6483049/94‑1 (MQ=60)

                                                 ACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAG                                            <  SRR4302545.5914490/94‑1 (MQ=60)

                                                  CTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGC                                           >  SRR4302545.5975870/1‑94 (MQ=60)

                                                   TCAATCACTAACTCATCGCCGCTGCCCATTTCTGCCAGAGCCGCCTTTGCTTCTATGAGTGGAAACGGACAGCCTTGCGTCACCACATTCAGCT                                          >  SRR4302545.9354413/1‑94 (MQ=60)

                                                    CAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTT                                         >  SRR4302545.4172983/1‑94 (MQ=60)

                                                    CAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTT                                         >  SRR4302545.2136218/1‑94 (MQ=60)

                                                    CAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTT                                         <  SRR4302545.995938/94‑1 (MQ=60)

                                                    AAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGAGTCACCACATCCAGCTT                                         <  SRR4302545.7045941/94‑1 (MQ=60)

                                                      ATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTT                                       <  SRR4302545.10697084/94‑1 (MQ=60)

                                                        CACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTA                                     <  SRR4302545.5822671/94‑1 (MQ=60)

                                                        CACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTA                                     <  SRR4302545.8659102/94‑1 (MQ=60)

                                                         ACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAA                                    <  SRR4302545.7786505/94‑1 (MQ=60)

                                                          CTAACTCATCGCCGCTGACCATTTCTGCCAGAGCTGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAAT                                   >  SRR4302545.7512044/1‑94 (MQ=60)

                                                           TAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATC                                  >  SRR4302545.3875444/1‑94 (MQ=60)

                                                           TAACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATC                                  >  SRR4302545.351002/1‑94 (MQ=60)

                                                            AACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCagcctttttattac                                 >  SRR4302545.7952445/1‑80 (MQ=60)

                                                            AACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCA                                 >  SRR4302545.5880204/1‑94 (MQ=60)

                                                            AACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCA                                 <  SRR4302545.4953100/94‑1 (MQ=60)

                                                             ACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCAC                                >  SRR4302545.1092681/1‑94 (MQ=60)

                                                             ACTCATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCAC                                >  SRR4302545.1658534/1‑94 (MQ=60)

                                                                CATCGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCAT                             >  SRR4302545.6354724/1‑94 (MQ=60)

                                                                   CGCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTT                          <  SRR4302545.8957231/94‑1 (MQ=60)

                                                                    GCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTT                         <  SRR4302545.6661975/94‑1 (MQ=60)

                                                                    GCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTT                         <  SRR4302545.8989904/94‑1 (MQ=60)

                                                                    GCCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTT                         >  SRR4302545.10810416/1‑94 (MQ=60)

                                                                     CCGCTGACCATTTCTGCCAGAGCTGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTA                        >  SRR4302545.5433544/1‑94 (MQ=60)

                                                                     CCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTA                        <  SRR4302545.10540694/94‑1 (MQ=60)

                                                                     CCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTA                        <  SRR4302545.10540695/94‑1 (MQ=60)

                                                                     CCGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTA                        <  SRR4302545.2696003/94‑1 (MQ=60)

                                                                      CGCTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTAA                       >  SRR4302545.8433231/1‑94 (MQ=60)

                                                                        CTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCTCCACATCCAGCTTTTTAATCACCATCTTTACAC                     <  SRR4302545.470772/94‑1 (MQ=60)

                                                                        CTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACAC                     >  SRR4302545.6514511/1‑94 (MQ=60)

                                                                        CTGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACAC                     >  SRR4302545.6036552/1‑94 (MQ=60)

                                                                         TGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACC                    >  SRR4302545.7102348/1‑94 (MQ=60)

                                                                         TGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACC                    >  SRR4302545.1837413/1‑94 (MQ=60)

                                                                         TGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACC                    >  SRR4302545.1837414/1‑94 (MQ=60)

                                                                         TGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACC                    <  SRR4302545.2524362/94‑1 (MQ=60)

                                                                         TGACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACC                    <  SRR4302545.4027104/94‑1 (MQ=60)

                                                                           GCCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACCTT                  <  SRR4302545.9970473/94‑1 (MQ=60)

                                                                           ACCATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACCTT                  <  SRR4302545.6881085/94‑1 (MQ=60)

                                                                             CATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACCTTAC                >  SRR4302545.8405375/1‑94 (MQ=60)

                                                                             CATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACCTTAC                >  SRR4302545.2409438/1‑94 (MQ=60)

                                                                              ATTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACCTTACT               >  SRR4302545.3078750/1‑94 (MQ=60)

                                                                               TTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACCTTACTT              <  SRR4302545.9171723/94‑1 (MQ=60)

                                                                               TTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACCTTACTT              <  SRR4302545.9171722/94‑1 (MQ=60)

                                                                               TTTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACCTTACTT              <  SRR4302545.4551159/94‑1 (MQ=60)

                                                                                TTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACCTTACTTA             <  SRR4302545.10119029/94‑1 (MQ=60)

                                                                                TTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACCTTACTTA             <  SRR4302545.5112306/94‑1 (MQ=60)

                                                                                TTCTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACCTTACTTA             <  SRR4302545.5285911/94‑1 (MQ=60)

                                                                                  CTGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACCTTACTTAAT           >  SRR4302545.4513190/1‑94 (MQ=60)

                                                                                   TGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACCTTACTTAATT          >  SRR4302545.5567967/1‑94 (MQ=60)

                                                                                   TGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACCTTACTTAATT          <  SRR4302545.10931515/94‑1 (MQ=60)

                                                                                   TGCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACCTTACTTAATT          >  SRR4302545.10817234/1‑94 (MQ=60)

                                                                                    GCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACCTTACTTAATTT         <  SRR4302545.4410488/94‑1 (MQ=60)

                                                                                    GCCAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACCTTACTTAATTT         <  SRR4302545.6911789/94‑1 (MQ=60)

                                                                                      CAGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCCGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACCTTACTTAATTTGC       >  SRR4302545.6149500/1‑94 (MQ=60)

                                                                                       AGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACGATCTTTACACATTACTTAATTTGCC      >  SRR4302545.4179714/1‑94 (MQ=60)

                                                                                       AGAGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACCTTACTTAATTTGCC      >  SRR4302545.687042/1‑94 (MQ=60)

                                                                                         AGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACCTTACTTAATTTGCCGC    >  SRR4302545.9090801/1‑94 (MQ=60)

                                                                                         AGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACCTTACTTAATTTGCCGC    >  SRR4302545.920253/1‑94 (MQ=60)

                                                                                         AGCCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACCTTACTTAATTTGCCGC    >  SRR4302545.2918061/1‑94 (MQ=60)

                                                                                           CCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACCTTACTTAATTTGCCGCAG  <  SRR4302545.3003495/94‑1 (MQ=60)

                                                                                           CCGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACCTTACTTAATTTGCCGCAG  >  SRR4302545.8819037/1‑94 (MQ=60)

TCTTCTGCCGCCCACTGTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCTGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACCTTACTTAATTTGCCGCAG  >  NC_000913/2082318‑2082502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`