Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,153,206 T→C V439A (GTG→GCG)  b2074 → putative membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,153,2060TC99.5% 614.2 / ‑5.7 183V439A (GTG→GCG) b2074putative membrane protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (88/94);  total (89/94)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 214 total aligned reads displayed.

CAGAAAGTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTA  >  NC_000913/2153115‑2153296
                                                                                           |                                                                                          
cAGAAAGTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAaagtggaagcgg                                                                                          <  2:3903538/94‑1 (MQ=255)
 aGAAAGTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAaagtggaagcggg                                                                                         >  1:6396897/1‑93 (MQ=255)
 aGAAAGTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAgcggt                                                                                         >  1:7288370/1‑94 (MQ=255)
  gAAAGTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAgcggtg                                                                                        >  2:7145698/1‑94 (MQ=255)
   aaaGTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGcggtgg                                                                                       <  2:2189729/94‑1 (MQ=255)
    aaGTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGa                                                                                      <  1:8872786/94‑1 (MQ=255)
     aGTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAGGCGGTGGaa                                                                                     <  1:7978103/94‑1 (MQ=255)
     aGTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGaa                                                                                     <  1:4870826/94‑1 (MQ=255)
      gtggtgATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAg                                                                                    >  2:9615204/1‑94 (MQ=255)
      gtggtgATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAg                                                                                    <  2:5410393/94‑1 (MQ=255)
      gtggtgATCCGTGCAGGAATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAg                                                                                    >  1:8443729/1‑94 (MQ=255)
       gggtgATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGc                                                                                   <  2:9342666/93‑1 (MQ=255)
         gtgATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGccc                                                                                 <  2:5157561/94‑1 (MQ=255)
          tgATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCa                                                                                <  1:4760328/94‑1 (MQ=255)
           gATCCGTGCAGGTGTTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCag                                                                               <  2:8055813/94‑1 (MQ=255)
           gATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCag                                                                               <  1:9071845/94‑1 (MQ=255)
             tCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCagag                                                                             >  1:8976276/1‑94 (MQ=255)
             tCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCagag                                                                             <  1:3089194/94‑1 (MQ=255)
             tCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCagag                                                                             <  2:7780794/94‑1 (MQ=255)
             tCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCagag                                                                             <  1:1738067/94‑1 (MQ=255)
              ccGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAgc                                                                            >  2:4632304/1‑94 (MQ=255)
              ccGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAgc                                                                            <  2:8685701/94‑1 (MQ=255)
              ccGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAgc                                                                            >  1:9553156/1‑94 (MQ=255)
               cGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAgcg                                                                           >  2:1929311/1‑94 (MQ=255)
               cGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAgcg                                                                           >  1:774027/1‑94 (MQ=255)
                   cAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCac                                                                       >  1:5952536/1‑94 (MQ=255)
                    aGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCact                                                                      <  1:9213331/94‑1 (MQ=255)
                     ggTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCacta                                                                     >  1:578075/1‑94 (MQ=255)
                     ggTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCacta                                                                     >  1:9968264/1‑94 (MQ=255)
                     ggTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCacta                                                                     <  1:7890890/94‑1 (MQ=255)
                      gTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCactac                                                                    >  1:7113725/1‑94 (MQ=255)
                        aTTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTc                                                                  >  1:394707/1‑94 (MQ=255)
                         tttCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTcc                                                                 <  1:3027516/94‑1 (MQ=255)
                          ttCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCg                                                                >  1:862396/1‑94 (MQ=255)
                           tCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCgg                                                               <  2:9160970/94‑1 (MQ=255)
                                gggCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAagag                                                          <  2:8468328/94‑1 (MQ=255)
                                gggCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAagag                                                          >  1:1025155/1‑94 (MQ=255)
                                gggCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAagag                                                          >  1:741187/1‑94 (MQ=255)
                                 ggCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACCCCGGAAgaga                                                         >  2:7380151/1‑94 (MQ=255)
                                 ggCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAgaga                                                         <  1:1302160/94‑1 (MQ=255)
                                 ggCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAgaga                                                         <  1:3380131/94‑1 (MQ=255)
                                 ggCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAgaga                                                         <  2:1079475/94‑1 (MQ=255)
                                  gCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGaa                                                        >  2:4172916/1‑94 (MQ=255)
                                  gCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGaa                                                        <  2:6239583/94‑1 (MQ=255)
                                   cGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGaaa                                                       >  2:7742141/1‑94 (MQ=255)
                                     aTCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGc                                                     >  1:6786231/1‑94 (MQ=255)
                                       cgcgTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGcca                                                   >  2:2538964/1‑94 (MQ=255)
                                       cgcgTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGcca                                                   >  2:4197489/1‑94 (MQ=255)
                                       cgcgTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGcca                                                   >  2:5017857/1‑94 (MQ=255)
                                       cgcgTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGcca                                                   >  1:8188295/1‑94 (MQ=255)
                                         cgTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGccacc                                                 <  2:9524684/94‑1 (MQ=255)
                                         cgTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGccacc                                                 <  2:4356163/94‑1 (MQ=255)
                                         cgTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGccacc                                                 >  1:5043679/1‑94 (MQ=255)
                                         cgTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGccacc                                                 <  1:5012917/94‑1 (MQ=255)
                                         cgTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGccacc                                                 >  2:8198598/1‑94 (MQ=255)
                                          gtggtgACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGccacca                                                >  2:640495/1‑94 (MQ=255)
                                          gtggtgACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGccacca                                                <  1:4085808/94‑1 (MQ=255)
                                           tggtgACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAg                                               <  2:9279414/94‑1 (MQ=255)
                                           tggtgACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAg                                               >  1:10124985/1‑94 (MQ=255)
                                           cggggACAGCAGGCATTGATCGCCTGACCGAAGGGGCGCAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAg                                               <  2:2612402/90‑1 (MQ=255)
                                             gtgACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGcc                                             <  2:1593483/94‑1 (MQ=255)
                                               gACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCcgc                                           <  2:5813165/94‑1 (MQ=255)
                                               gACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAACCCACCAGCcgc                                           >  2:5385002/1‑94 (MQ=255)
                                                  aGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGaa                                        >  1:1892775/1‑94 (MQ=255)
                                                  aGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGaa                                        >  1:1514418/1‑94 (MQ=255)
                                                   gACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAAt                                       <  2:4559168/94‑1 (MQ=255)
                                                     cGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATAc                                     <  1:3283829/94‑1 (MQ=255)
                                                     cGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACAAGCCGCGAATAc                                     >  1:8964365/1‑94 (MQ=255)
                                                      ggCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATAcg                                    >  2:422618/1‑94 (MQ=255)
                                                      ggCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATAcg                                    >  2:5903365/1‑94 (MQ=255)
                                                       gCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAGGAGAAAGCCACCAGCCGCGAATAcgc                                   >  1:1041712/1‑94 (MQ=255)
                                                        cATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATAcgcg                                  <  1:1970485/94‑1 (MQ=255)
                                                        cATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATAcgcg                                  <  2:6352971/94‑1 (MQ=255)
                                                        cATGGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATAcgcg                                  <  2:3635323/94‑1 (MQ=255)
                                                         aTTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGa                                 <  1:2574988/94‑1 (MQ=255)
                                                             aTCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGaaaaa                             <  1:4384702/94‑1 (MQ=255)
                                                               cGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAg                           >  2:7113413/1‑94 (MQ=255)
                                                                gCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAgg                          <  2:10245456/94‑1 (MQ=255)
                                                                  cTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAg                        <  1:10302683/94‑1 (MQ=255)
                                                                  cTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAg                        <  2:6085709/94‑1 (MQ=255)
                                                                   tGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCAACCAGCCGCGAATACGCGAAAAAAGGAGc                       >  2:3813718/1‑94 (MQ=255)
                                                                    gACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCa                      <  2:3211603/94‑1 (MQ=255)
                                                                    gACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCa                      >  2:7557133/1‑94 (MQ=255)
                                                                     aCCGAAGGGGAGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCAc                     <  2:2844715/94‑1 (MQ=255)
                                                                       cGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGc                   <  1:3266316/94‑1 (MQ=255)
                                                                        gAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGTATACGCGAAAAAAGGAGCACGCt                  <  2:10126991/94‑1 (MQ=255)
                                                                        gAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCt                  <  1:7916211/94‑1 (MQ=255)
                                                                         aaGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTc                 >  1:6903860/1‑94 (MQ=255)
                                                                         aaGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTc                 >  2:126952/1‑94 (MQ=255)
                                                                            gggCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTg              >  1:6704396/1‑94 (MQ=255)
                                                                                gAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGc          >  1:5561272/1‑94 (MQ=255)
                                                                                gAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGc          >  2:1448771/1‑94 (MQ=255)
                                                                                   agtggaagcggTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAgg       >  1:1998557/1‑94 (MQ=255)
                                                                                    gtggaagcggTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGgt      <  2:7163037/94‑1 (MQ=255)
                                                                                    gtggaagcggTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGgt      <  2:7821467/94‑1 (MQ=255)
                                                                                     tggaaacggTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGgtg     <  1:2246455/94‑1 (MQ=255)
                                                                                        aagcggTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTa  >  1:3532070/1‑94 (MQ=255)
                                                                                        aagcggTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTa  <  2:6879150/94‑1 (MQ=255)
                                                                                        aagcggTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTa  <  2:8946492/94‑1 (MQ=255)
                                                                                        aagcggTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTa  <  1:10008593/94‑1 (MQ=255)
                                                                                           |                                                                                          
CAGAAAGTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTA  >  NC_000913/2153115‑2153296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCAGAAAGTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCC  >  NC_000913/2153114‑2153299
                                                                                            |                                                                                             
TCAGAAAGTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCG                                                                                              >  SRR4302545.7399858/1‑94 (MQ=60)
  AGAAAGTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGCAGCGGT                                                                                            >  SRR4302545.8488429/1‑94 (MQ=60)
  AGAAAGTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGT                                                                                            <  SRR4302545.2161062/94‑1 (MQ=60)
  AGAAAGTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGT                                                                                            >  SRR4302545.9030424/1‑94 (MQ=60)
  AGAAAGTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGT                                                                                            >  SRR4302545.7492772/1‑94 (MQ=60)
     AAGTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGA                                                                                         <  SRR4302545.9130550/94‑1 (MQ=60)
      AGTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAGGCGGTGGAA                                                                                        <  SRR4302545.8204950/94‑1 (MQ=60)
      AGTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAA                                                                                        >  SRR4302545.4815017/1‑94 (MQ=60)
      AGTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAA                                                                                        <  SRR4302545.4994709/94‑1 (MQ=60)
       GTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAG                                                                                       <  SRR4302545.316969/94‑1 (MQ=60)
       GTGGTGATCCGTGCAGGAATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAG                                                                                       >  SRR4302545.8687843/1‑94 (MQ=60)
         GGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCC                                                                                     <  SRR4302545.281009/94‑1 (MQ=60)
          GTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCC                                                                                    <  SRR4302545.6595332/94‑1 (MQ=60)
           TGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCA                                                                                   >  SRR4302545.10178505/1‑94 (MQ=60)
           TGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCA                                                                                   <  SRR4302545.4881954/94‑1 (MQ=60)
            GATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAG                                                                                  <  SRR4302545.9337008/94‑1 (MQ=60)
             ATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGA                                                                                 >  SRR4302545.455228/1‑94 (MQ=60)
              TCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAG                                                                                <  SRR4302545.3165232/94‑1 (MQ=60)
              TCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAG                                                                                <  SRR4302545.1779615/94‑1 (MQ=60)
              TCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAG                                                                                <  SRR4302545.6950648/94‑1 (MQ=60)
              TCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAG                                                                                >  SRR4302545.9237449/1‑94 (MQ=60)
               CCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGC                                                                               >  SRR4302545.9834654/1‑94 (MQ=60)
                CGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCG                                                                              >  SRR4302545.791424/1‑94 (MQ=60)
                CGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCG                                                                              >  SRR4302545.9236811/1‑94 (MQ=60)
                CGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCG                                                                              >  SRR4302545.1042519/1‑94 (MQ=60)
                CGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCG                                                                              >  SRR4302545.8579327/1‑94 (MQ=60)
                 GTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACCGAGGGCCTTGATCGCCTGACCGAAGGGGCGACAGTGGAAGGGGTGGAAGCCCAGcgggc                                                                             >  SRR4302545.3168151/1‑89 (MQ=60)
                 GTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGGGGAAGCGGTGGAAGCCCAGAGCGC                                                                             >  SRR4302545.6383075/1‑94 (MQ=60)
                  TGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCC                                                                            >  SRR4302545.5286388/1‑94 (MQ=60)
                  TGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCC                                                                            >  SRR4302545.8151267/1‑94 (MQ=60)
                    CAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCAC                                                                          >  SRR4302545.6113129/1‑94 (MQ=60)
                    CAGGTATTTCTGCGAGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACTGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCAC                                                                          <  SRR4302545.1726707/94‑1 (MQ=60)
                     AGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACT                                                                         <  SRR4302545.9483406/94‑1 (MQ=60)
                      GGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTA                                                                        >  SRR4302545.591121/1‑94 (MQ=60)
                      GGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTA                                                                        >  SRR4302545.10263966/1‑94 (MQ=60)
                      GGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTA                                                                        <  SRR4302545.8114814/94‑1 (MQ=60)
                       GTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTAC                                                                       <  SRR4302545.9345659/94‑1 (MQ=60)
                       GTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTAC                                                                       >  SRR4302545.7310859/1‑94 (MQ=60)
                        TATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCACAGAGCGCCACTAGT                                                                      >  SRR4302545.10672722/1‑94 (MQ=60)
                         ATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTC                                                                     >  SRR4302545.323505/1‑94 (MQ=60)
                         ATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTC                                                                     >  SRR4302545.3386871/1‑94 (MQ=60)
                         ATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTC                                                                     >  SRR4302545.403489/1‑94 (MQ=60)
                          TTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCC                                                                    <  SRR4302545.3101621/94‑1 (MQ=60)
                          TTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCC                                                                    <  SRR4302545.4247299/94‑1 (MQ=60)
                          TTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCC                                                                    <  SRR4302545.1974916/94‑1 (MQ=60)
                           TTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCG                                                                   >  SRR4302545.881740/1‑94 (MQ=60)
                                 GGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAG                                                             <  SRR4302545.4892346/94‑1 (MQ=60)
                                 GGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAG                                                             >  SRR4302545.757845/1‑94 (MQ=60)
                                 GGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAG                                                             >  SRR4302545.1047683/1‑94 (MQ=60)
                                  GGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGA                                                            <  SRR4302545.1331075/94‑1 (MQ=60)
                                  GGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGA                                                            >  SRR4302545.1862938/1‑94 (MQ=60)
                                  GGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGA                                                            <  SRR4302545.3462516/94‑1 (MQ=60)
                                    CGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAA                                                          >  SRR4302545.8126117/1‑94 (MQ=60)
                                      ATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGC                                                        >  SRR4302545.6972464/1‑94 (MQ=60)
                                      ATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGC                                                        >  SRR4302545.6972462/1‑94 (MQ=60)
                                        CGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCA                                                      >  SRR4302545.9092911/1‑94 (MQ=60)
                                        CGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCA                                                      >  SRR4302545.8425240/1‑94 (MQ=60)
                                         GCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCAC                                                     <  SRR4302545.10718366/94‑1 (MQ=60)
                                          CGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACC                                                    <  SRR4302545.5140010/94‑1 (MQ=60)
                                          CGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACC                                                    >  SRR4302545.5171808/1‑94 (MQ=60)
                                          CGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACC                                                    >  SRR4302545.5171809/1‑94 (MQ=60)
                                           GTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCA                                                   <  SRR4302545.4188115/94‑1 (MQ=60)
                                           GTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCA                                                   <  SRR4302545.3358878/94‑1 (MQ=60)
                                            TGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAG                                                  >  SRR4302545.10424415/1‑94 (MQ=60)
                                             GGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGC                                                 <  SRR4302545.7128710/94‑1 (MQ=60)
                                                GACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGC                                              >  SRR4302545.777021/1‑94 (MQ=60)
                                                 ACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCG                                             >  SRR4302545.10589454/1‑94 (MQ=60)
                                                   AGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAA                                           >  SRR4302545.1939411/1‑94 (MQ=60)
                                                   AGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAA                                           >  SRR4302545.1939406/1‑94 (MQ=60)
                                                   AGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAA                                           >  SRR4302545.1549731/1‑94 (MQ=60)
                                                    GACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAAT                                          <  SRR4302545.7639118/94‑1 (MQ=60)
                                                      CGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATAC                                        <  SRR4302545.3364273/94‑1 (MQ=60)
                                                      CGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATAC                                        >  SRR4302545.9225037/1‑94 (MQ=60)
                                                      CGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATAC                                        <  SRR4302545.3719729/94‑1 (MQ=60)
                                                      CGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACAAGCCGCGAATAC                                        >  SRR4302545.9225038/1‑94 (MQ=60)
                                                       GGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACG                                       >  SRR4302545.10275896/1‑94 (MQ=60)
                                                       GGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCAACTACTCCGGAAGAACAAGCCACCAGCCGCGCATACG                                       >  SRR4302545.10275897/1‑94 (MQ=60)
                                                        GCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAGGAGAAAGCCACCAGCCGCGAATACGC                                      >  SRR4302545.1064460/1‑94 (MQ=60)
                                                         CATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCG                                     <  SRR4302545.2019321/94‑1 (MQ=60)
                                                          ATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGA                                    <  SRR4302545.2637007/94‑1 (MQ=60)
                                                              ATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAA                                >  SRR4302545.10307611/1‑94 (MQ=60)
                                                              ATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAA                                <  SRR4302545.4495747/94‑1 (MQ=60)
                                                              ATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACGACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAA                                <  SRR4302545.3118247/94‑1 (MQ=60)
                                                                 GCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGG                             >  SRR4302545.8225163/1‑94 (MQ=60)
                                                                 GCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGG                             <  SRR4302545.8074406/94‑1 (MQ=60)
                                                                  CCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGA                            <  SRR4302545.750846/94‑1 (MQ=60)
                                                                   CTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCCCCAGCCGCGAATACGCGAAAAAAGGAG                           <  SRR4302545.2030854/94‑1 (MQ=60)
                                                                   CTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAG                           <  SRR4302545.10606520/94‑1 (MQ=60)
                                                                      CCCGAAGGGGCGAAAGTGGAAGCGATGGAAGCCCAGAGCGCCAATACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCAC                        <  SRR4302545.6352478/94‑1 (MQ=60)
                                                                      ACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCAC                        <  SRR4302545.404928/94‑1 (MQ=60)
                                                                       CCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACG                       <  SRR4302545.648541/94‑1 (MQ=60)
                                                                        CGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGC                      <  SRR4302545.3346407/94‑1 (MQ=60)
                                                                         GAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCT                     >  SRR4302545.10803883/1‑94 (MQ=60)
                                                                         GAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCT                     <  SRR4302545.8140954/94‑1 (MQ=60)
                                                                         GAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCT                     >  SRR4302545.1362077/1‑94 (MQ=60)
                                                                          AAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTC                    >  SRR4302545.7094033/1‑94 (MQ=60)
                                                                             GGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTG                 >  SRR4302545.6888228/1‑94 (MQ=60)
                                                                              GGCGAAAGGGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGA                <  SRR4302545.2326583/94‑1 (MQ=60)
                                                                                CGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATG              <  SRR4302545.348870/94‑1 (MQ=60)
                                                                                 GAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGC             >  SRR4302545.5708401/1‑94 (MQ=60)
                                                                                  AAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCA            <  SRR4302545.5798702/94‑1 (MQ=60)
                                                                                  AAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCA            <  SRR4302545.605662/94‑1 (MQ=60)
                                                                                    AGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGG          >  SRR4302545.2047992/1‑94 (MQ=60)
                                                                                     GTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGT         <  SRR4302545.7363234/94‑1 (MQ=60)
                                                                                      TGGAAACGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTG        <  SRR4302545.2301171/94‑1 (MQ=60)
                                                                                       GGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGT       >  SRR4302545.922184/1‑94 (MQ=60)
                                                                                        GAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTT      <  SRR4302545.8743497/94‑1 (MQ=60)
                                                                                         AAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTA     <  SRR4302545.10305297/94‑1 (MQ=60)
                                                                                         AAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTA     >  SRR4302545.3618611/1‑94 (MQ=60)
                                                                                          AGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTAC    <  SRR4302545.60448/94‑1 (MQ=60)
                                                                                          AGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTAC    <  SRR4302545.3496110/94‑1 (MQ=60)
                                                                                          AGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTAC    <  SRR4302545.3903569/94‑1 (MQ=60)
                                                                                           GCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACC   <  SRR4302545.10545829/94‑1 (MQ=60)
                                                                                           GCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACC   <  SRR4302545.3759039/94‑1 (MQ=60)
                                                                                           GCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACC   <  SRR4302545.9717918/94‑1 (MQ=60)
                                                                                            CGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCC  <  SRR4302545.8206076/94‑1 (MQ=60)
                                                                                            |                                                                                             
TCAGAAAGTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCC  >  NC_000913/2153114‑2153299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑