Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,153,206 T→C V439A (GTG→GCG)  b2074 → putative membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,153,2060TC100.0% 514.9 / NA 150V439A (GTG→GCG) b2074putative membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (68/82);  total (68/82)

Only 100 of 179 total aligned reads displayed.

AGAAAGTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTT  >  NC_000913/2153116‑2153295
                                                                                          |                                                                                         
aGAAAGTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAaagtggaagcgg                                                                                         <  1:1404511/93‑1 (MQ=255)
aGAAAGTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAaagtggaagcgg                                                                                         <  1:185936/93‑1 (MQ=255)
 gAAAGTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAaagtggaagcggg                                                                                        >  2:806242/1‑92 (MQ=255)
 gAAAGTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAaagtggaagcggg                                                                                        >  2:5811982/1‑92 (MQ=255)
 gAAAGTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAgcggt                                                                                        <  1:3372339/93‑1 (MQ=255)
    aGTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGa                                                                                     <  1:59550/93‑1 (MQ=255)
    aGTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGa                                                                                     <  2:3062065/93‑1 (MQ=255)
       ggtgATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGc                                                                                  <  2:1564976/93‑1 (MQ=255)
         tgATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGccc                                                                                >  1:4766140/1‑93 (MQ=255)
          gATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCa                                                                               <  2:1062775/93‑1 (MQ=255)
          gATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCa                                                                               <  1:2456125/93‑1 (MQ=255)
           aTCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCag                                                                              >  1:1536940/1‑93 (MQ=255)
           aTCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCag                                                                              <  1:2666875/93‑1 (MQ=255)
           aTCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCag                                                                              >  2:581667/1‑93 (MQ=255)
           aTCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCag                                                                              <  1:4970097/93‑1 (MQ=255)
            tCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCaga                                                                             <  2:3465202/93‑1 (MQ=255)
            tCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCaga                                                                             <  2:5419370/93‑1 (MQ=255)
             ccGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCagag                                                                            >  1:2616511/1‑93 (MQ=255)
             ccGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCagag                                                                            >  1:4610367/1‑93 (MQ=255)
              cGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAgcgc                                                                           >  1:6185561/1‑93 (MQ=255)
                tGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAgcgc                                                                         >  1:2945975/1‑93 (MQ=255)
                 gCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGcc                                                                        >  1:2354582/1‑93 (MQ=255)
                 gCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGcc                                                                        >  1:3645428/1‑93 (MQ=255)
                   aGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCac                                                                      <  2:5727156/93‑1 (MQ=255)
                   aGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCac                                                                      <  2:5079937/93‑1 (MQ=255)
                   aGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCac                                                                      >  1:3914301/1‑93 (MQ=255)
                   aGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCac                                                                      <  1:399792/93‑1 (MQ=255)
                   aGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCac                                                                      >  2:411430/1‑93 (MQ=255)
                    ggTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCact                                                                     >  2:3510132/1‑93 (MQ=255)
                     gTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCacta                                                                    <  1:509201/93‑1 (MQ=255)
                     gTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCacta                                                                    <  1:5043589/93‑1 (MQ=255)
                      tATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCactac                                                                   >  2:6343058/1‑93 (MQ=255)
                      tATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCactac                                                                   >  1:2118803/1‑93 (MQ=255)
                       aTTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCactact                                                                  >  1:641174/1‑93 (MQ=255)
                        tttCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTc                                                                 >  1:2872272/1‑93 (MQ=255)
                        tttCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTc                                                                 <  1:5283230/93‑1 (MQ=255)
                        tttCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTc                                                                 >  1:5509399/1‑93 (MQ=255)
                         ttCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTcc                                                                <  1:3314217/93‑1 (MQ=255)
                         ttCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTcc                                                                <  2:4793605/93‑1 (MQ=255)
                           cTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCgg                                                              <  2:2459922/93‑1 (MQ=255)
                               gggCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAaga                                                          <  1:773911/93‑1 (MQ=255)
                               gggCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAaga                                                          <  1:4258121/93‑1 (MQ=255)
                                ggCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAagag                                                         <  2:2123449/93‑1 (MQ=255)
                                 gCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAgaga                                                        >  1:3238685/1‑93 (MQ=255)
                                 gCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAgaga                                                        >  1:3259073/1‑93 (MQ=255)
                                  cGATCGCGTGGTAACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGaa                                                       <  1:4486119/93‑1 (MQ=255)
                                   gATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGaaa                                                      <  1:1579645/93‑1 (MQ=255)
                                   gATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGaaa                                                      <  2:1754429/93‑1 (MQ=255)
                                   gATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGACCAGAGCGCCACTACTCCGGAAGAGaaa                                                      >  1:472246/1‑93 (MQ=255)
                                    aTCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGaaaa                                                     >  2:6184527/1‑92 (MQ=255)
                                     tCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGc                                                    <  2:5549323/93‑1 (MQ=255)
                                         gtggtgACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGccacc                                                <  1:5403738/93‑1 (MQ=255)
                                         gtggtgACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGccacc                                                >  1:5024431/1‑93 (MQ=255)
                                          tggtgACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGccacca                                               >  1:827942/1‑93 (MQ=255)
                                          tggtgACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGccacca                                               <  1:913499/93‑1 (MQ=255)
                                           ggtgACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAg                                              <  1:1247330/93‑1 (MQ=255)
                                           ggtgACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAg                                              <  1:5265467/93‑1 (MQ=255)
                                            gtgACAGCCGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGc                                             <  2:1895488/93‑1 (MQ=255)
                                            gtgACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGc                                             >  1:4357833/1‑93 (MQ=255)
                                              gACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCcg                                           <  2:2935935/93‑1 (MQ=255)
                                                cAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCcgcg                                         >  2:2646577/1‑93 (MQ=255)
                                                 aGACGCCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGa                                        <  2:2158473/93‑1 (MQ=255)
                                                  gACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGaa                                       >  2:1179441/1‑93 (MQ=255)
                                                  gACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGaa                                       <  2:4706772/93‑1 (MQ=255)
                                                    cGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATa                                     <  1:4187017/93‑1 (MQ=255)
                                                    cGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATa                                     <  2:3770744/93‑1 (MQ=255)
                                                     ggCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATAc                                    <  1:5653520/93‑1 (MQ=255)
                                                     ggCATTGATCGCCTGACCGAAGGGCCGAAAGTGGAAGCGGTGGAACCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATAc                                    <  2:391856/93‑1 (MQ=255)
                                                      gCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATAcg                                   <  2:55887/93‑1 (MQ=255)
                                                         ttGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGa                                >  2:727637/1‑93 (MQ=255)
                                                          tGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGaa                               <  2:900651/93‑1 (MQ=255)
                                                           gATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTCCTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGaaa                              <  2:4978026/93‑1 (MQ=255)
                                                           gATCGCCTGACCGAAGGGGAGAAAGTGGAAGCGGTGGAAGCCGAGAGCGCCACTACTCCGGAAGAGAAAGCAACCAGCCGCGAATACGCGaaa                              <  1:819111/93‑1 (MQ=255)
                                                            aTCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGaaaa                             >  2:5849682/1‑93 (MQ=255)
                                                            aTCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGaaaa                             <  2:4578742/93‑1 (MQ=255)
                                                            aTCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGaaaa                             >  2:2865828/1‑93 (MQ=255)
                                                               gCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAg                          >  1:2153743/1‑93 (MQ=255)
                                                               gCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAg                          >  1:2819745/1‑93 (MQ=255)
                                                                 cTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGa                        <  2:3639578/93‑1 (MQ=255)
                                                                 cTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGa                        >  2:2953228/1‑93 (MQ=255)
                                                                  tGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAg                       >  2:4178810/1‑93 (MQ=255)
                                                                   gACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGc                      <  2:4766140/93‑1 (MQ=255)
                                                                    aCCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCa                     >  2:6335592/1‑93 (MQ=255)
                                                                         aGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTc                >  2:153817/1‑93 (MQ=255)
                                                                          ggggCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTcc               <  2:541273/93‑1 (MQ=255)
                                                                           gggCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCt              <  1:483755/93‑1 (MQ=255)
                                                                            ggCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTg             >  1:3469271/1‑93 (MQ=255)
                                                                             gCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGa            <  1:1280893/93‑1 (MQ=255)
                                                                              cGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGAt           >  2:5004113/1‑93 (MQ=255)
                                                                              cGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGAt           >  1:5732334/1‑93 (MQ=255)
                                                                                 aagtggaagcggTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCa        <  1:1051464/93‑1 (MQ=255)
                                                                                  agtggaagcggTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAg       <  1:3552732/93‑1 (MQ=255)
                                                                                  agtggaagcggTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAg       >  2:3902423/1‑93 (MQ=255)
                                                                                   gtggaagcggTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAgg      >  2:4253197/1‑93 (MQ=255)
                                                                                    tggaagcggTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGgt     <  1:3506934/93‑1 (MQ=255)
                                                                                    tggaagcggTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGgt     <  1:4167653/93‑1 (MQ=255)
                                                                                    tggaagcggTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGgt     <  1:4778396/93‑1 (MQ=255)
                                                                                    gggaagcggTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGgt     <  1:212931/92‑1 (MQ=255)
                                                                                       aagcggTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGtt  >  1:3575089/1‑93 (MQ=255)
                                                                                       aagcggTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGtt  >  2:6097819/1‑93 (MQ=255)
                                                                                          |                                                                                         
AGAAAGTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTT  >  NC_000913/2153116‑2153295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTCAGAAAGTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTT  >  NC_000913/2153113‑2153295
                                                                                             |                                                                                         
GTCAGAAAGTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGC                                                                                           <  SRR4302537.1680438/94‑1 (MQ=60)
  CAGAAAGTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGG                                                                                         <  SRR4302537.4700580/94‑1 (MQ=60)
   AGAAAGTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGT                                                                                        <  SRR4302537.1414292/94‑1 (MQ=60)
   AGAAAGTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGT                                                                                        <  SRR4302537.187257/94‑1 (MQ=60)
    GAAAGTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTG                                                                                       <  SRR4302537.3396237/94‑1 (MQ=60)
       AGTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAA                                                                                    <  SRR4302537.59989/94‑1 (MQ=60)
         TGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGC                                                                                  <  SRR4302537.690912/94‑1 (MQ=60)
           GTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCC                                                                                >  SRR4302537.4799056/1‑94 (MQ=60)
             GATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAG                                                                              <  SRR4302537.2473400/94‑1 (MQ=60)
             GATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAG                                                                              <  SRR4302537.4217224/94‑1 (MQ=60)
             GATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAG                                                                              >  SRR4302537.1547676/1‑94 (MQ=60)
              ATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGA                                                                             <  SRR4302537.2685507/94‑1 (MQ=60)
              ATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGA                                                                             >  SRR4302537.3397618/1‑94 (MQ=60)
              ATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGA                                                                             <  SRR4302537.5004350/94‑1 (MQ=60)
               TCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAG                                                                            >  SRR4302537.4642291/1‑94 (MQ=60)
               TCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAG                                                                            <  SRR4302537.889876/94‑1 (MQ=60)
               TCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAG                                                                            >  SRR4302537.2634810/1‑94 (MQ=60)
                CCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGCGC                                                                           >  SRR4302537.6228170/1‑94 (MQ=60)
                CCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGC                                                                           >  SRR4302537.5508702/1‑94 (MQ=60)
                 CGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCG                                                                          <  SRR4302537.4808307/94‑1 (MQ=60)
                  GTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGC                                                                         >  SRR4302537.2966654/1‑94 (MQ=60)
                   TGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCC                                                                        >  SRR4302537.2371183/1‑94 (MQ=60)
                   TGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCC                                                                        >  SRR4302537.3671094/1‑94 (MQ=60)
                    GCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCA                                                                       <  SRR4302537.3274890/94‑1 (MQ=60)
                     CAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCAC                                                                      >  SRR4302537.3941665/1‑94 (MQ=60)
                      AGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACT                                                                     <  SRR4302537.402563/94‑1 (MQ=60)
                      AGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACT                                                                     <  SRR4302537.3330959/94‑1 (MQ=60)
                       GGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTA                                                                    >  SRR4302537.5265189/1‑94 (MQ=60)
                        GTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTAC                                                                   >  SRR4302537.2133719/1‑94 (MQ=60)
                        GTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTAC                                                                   <  SRR4302537.5078326/94‑1 (MQ=60)
                        GTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTAC                                                                   <  SRR4302537.512699/94‑1 (MQ=60)
                         TATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACT                                                                  >  SRR4302537.645544/1‑94 (MQ=60)
                          ATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTC                                                                 >  SRR4302537.2892430/1‑94 (MQ=60)
                          ATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTC                                                                 >  SRR4302537.5547024/1‑94 (MQ=60)
                           TTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCC                                                                <  SRR4302537.5319566/94‑1 (MQ=60)
                            TTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCG                                                               <  SRR4302537.3337660/94‑1 (MQ=60)
                             TCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGG                                                              <  SRR4302537.4765790/94‑1 (MQ=60)
                             TCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGG                                                              >  SRR4302537.2928495/1‑94 (MQ=60)
                              CTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGA                                                             >  SRR4302537.4550536/1‑94 (MQ=60)
                                GCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAG                                                           <  SRR4302537.4511478/94‑1 (MQ=60)
                                 CGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGA                                                          >  SRR4302537.2555574/1‑94 (MQ=60)
                                  GGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAG                                                         <  SRR4302537.4287711/94‑1 (MQ=60)
                                  GGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAG                                                         <  SRR4302537.779213/94‑1 (MQ=60)
                                   GGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGA                                                        >  SRR4302537.3282054/1‑94 (MQ=60)
                                   GGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGA                                                        >  SRR4302537.3261516/1‑94 (MQ=60)
                                    GCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAA                                                       >  SRR4302537.4942154/1‑94 (MQ=60)
                                     CGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGACCAGAGCGCCACTACTCCGGAAGAGAAA                                                      >  SRR4302537.475480/1‑94 (MQ=60)
                                     CGATCGCGTGGTAACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAA                                                      <  SRR4302537.4517151/94‑1 (MQ=60)
                                      GATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAG                                                     >  SRR4302537.5110429/1‑94 (MQ=60)
                                      GATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAG                                                     <  SRR4302537.1590682/94‑1 (MQ=60)
                                       ATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGC                                                    >  SRR4302537.4097084/1‑94 (MQ=60)
                                           CGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACC                                                >  SRR4302537.5059038/1‑94 (MQ=60)
                                            GTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCA                                               <  SRR4302537.5440608/94‑1 (MQ=60)
                                            GTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCA                                               >  SRR4302537.5702898/1‑94 (MQ=60)
                                            GTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCA                                               >  SRR4302537.833612/1‑94 (MQ=60)
                                             TGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAG                                              <  SRR4302537.919805/94‑1 (MQ=60)
                                              GGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGC                                             <  SRR4302537.5301699/94‑1 (MQ=60)
                                              GGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGC                                             <  SRR4302537.1256016/94‑1 (MQ=60)
                                              GGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGC                                             >  SRR4302537.4387995/1‑94 (MQ=60)
                                                   CAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGA                                        >  SRR4302537.99371/1‑94 (MQ=60)
                                                     GACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAAT                                      <  SRR4302537.4824958/94‑1 (MQ=60)
                                                       CGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATAC                                    <  SRR4302537.4216249/94‑1 (MQ=60)
                                                       CGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATAC                                    <  SRR4302537.2472981/94‑1 (MQ=60)
                                                        GGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACG                                   <  SRR4302537.5692211/94‑1 (MQ=60)
                                                        GGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACG                                   <  SRR4302537.2699616/94‑1 (MQ=60)
                                                          CATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCG                                 <  SRR4302537.628559/94‑1 (MQ=60)
                                                           ATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGA                                >  SRR4302537.3561061/1‑94 (MQ=60)
                                                            TTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAA                               <  SRR4302537.2137512/94‑1 (MQ=60)
                                                              GATCGCCTGACCGAAGGGGAGAAAGTGGAAGCGGTGGAAGCCGAGAGCGCCACTACTCCGGAAGAGAAAGCAACCAGCCGCGAATACGCGAAAA                             <  SRR4302537.824736/94‑1 (MQ=60)
                                                                 CGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAG                          >  SRR4302537.2839462/1‑94 (MQ=60)
                                                                 CGCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAG                          >  SRR4302537.2168926/1‑94 (MQ=60)
                                                                  GCCTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGG                         <  SRR4302537.1914707/94‑1 (MQ=60)
                                                                    CTGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAG                       >  SRR4302537.5550996/1‑94 (MQ=60)
                                                                     TGACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGC                      <  SRR4302537.1268951/94‑1 (MQ=60)
                                                                      GACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGGCCAGAGCGCCACTACTCCGGAAGAGAAAGACACCAGCCGCGAATACGCGAAAAAAGGAGCA                     >  SRR4302537.369354/1‑94 (MQ=60)
                                                                       ACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCAC                    <  SRR4302537.1396634/94‑1 (MQ=60)
                                                                       ACCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCAC                    >  SRR4302537.1512283/1‑94 (MQ=60)
                                                                       ACCGAAGGAGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCAC                    <  SRR4302537.2650052/94‑1 (MQ=60)
                                                                        CCGAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACG                   >  SRR4302537.4012897/1‑94 (MQ=60)
                                                                          GAAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCT                 >  SRR4302537.5073963/1‑94 (MQ=60)
                                                                           AAGGGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTC                >  SRR4302537.6161687/1‑94 (MQ=60)
                                                                              GGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTG             <  SRR4302537.487070/94‑1 (MQ=60)
                                                                              GGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTG             >  SRR4302537.3493813/1‑94 (MQ=60)
                                                                              GGGCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTG             <  SRR4302537.3507209/94‑1 (MQ=60)
                                                                                GCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGAT           <  SRR4302537.1857057/94‑1 (MQ=60)
                                                                                GCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGAT           >  SRR4302537.5771578/1‑94 (MQ=60)
                                                                                GCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGAT           <  SRR4302537.811774/94‑1 (MQ=60)
                                                                                GCGAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGAT           <  SRR4302537.1289798/94‑1 (MQ=60)
                                                                                  GAAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGC         >  SRR4302537.3632810/1‑94 (MQ=60)
                                                                                   AAAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCA        <  SRR4302537.4372654/94‑1 (MQ=60)
                                                                                    AAGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAG       <  SRR4302537.1058717/94‑1 (MQ=60)
                                                                                     AGTGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGG      <  SRR4302537.3577817/94‑1 (MQ=60)
                                                                                       TGGAAGCGGTGGAAGCCCAGAGCGCCGCTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTG    <  SRR4302537.2996241/94‑1 (MQ=60)
                                                                                       TGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTG    <  SRR4302537.5358224/94‑1 (MQ=60)
                                                                                       TGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTG    <  SRR4302537.3531745/94‑1 (MQ=60)
                                                                                       TGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTG    <  SRR4302537.4811385/94‑1 (MQ=60)
                                                                                       TGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTG    <  SRR4302537.4196763/94‑1 (MQ=60)
                                                                                       GGGAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTG    <  SRR4302537.214450/94‑1 (MQ=60)
                                                                                         GAAGCGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTT  >  SRR4302537.3600301/1‑94 (MQ=60)
                                                                                             |                                                                                         
GTCAGAAAGTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTT  >  NC_000913/2153113‑2153295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑