Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	647	270.1	21258240	98.4%	20918108	94.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,216,746	G→C	S252S (TCC→TCG)	yehZ ←	putative transport system permease protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,216,746	0	G	C	99.5%	665.8 / ‑5.0	195	S252S (TCC→TCG)	yehZ	putative transport system permease protein
Reads supporting (aligned to +/- strand): ref base G (0/0); major base C (84/110); minor base T (0/1); total (84/111)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

Only 100 of 206 total aligned reads displayed.

TGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACGGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGCCG  >  NC_000913/2216655‑2216836

  gtGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACt                                                                                        <  2:2877274/93‑1 (MQ=255)

  cTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACt                                                                                        <  1:4206383/94‑1 (MQ=255)

    ggCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTTCCTGAACACCGACTcc                                                                                      >  2:4350758/1‑93 (MQ=255)

    ggCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATAATCCCTCAACACCGACTcc                                                                                      >  1:87596/1‑93 (MQ=255)

     gCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCAc                                                                                     <  2:9626866/94‑1 (MQ=255)

      cATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACg                                                                                    <  2:7479135/94‑1 (MQ=255)

      cATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACg                                                                                    >  2:1752102/1‑94 (MQ=255)

       aTTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGc                                                                                   >  2:7300136/1‑94 (MQ=255)

       aTTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACCCACGc                                                                                   >  1:7388478/1‑94 (MQ=255)

        ttCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGca                                                                                  >  1:5548400/1‑94 (MQ=255)

         tCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGcac                                                                                 <  2:4066882/94‑1 (MQ=255)

           aGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGcaccc                                                                               >  1:377025/1‑93 (MQ=255)

            gTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGcaccac                                                                              <  2:9937622/94‑1 (MQ=255)

            gTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGcaccac                                                                              >  1:2656733/1‑94 (MQ=255)

             ttGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACt                                                                             <  1:3289380/94‑1 (MQ=255)

               gctgcAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTgg                                                                           >  2:6489751/1‑94 (MQ=255)

                 ggcAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTGGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCGCTGGTg                                                                         <  2:6531592/93‑1 (MQ=255)

                  gcAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGc                                                                        >  1:9248033/1‑94 (MQ=255)

                  gcAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGc                                                                        >  1:7085032/1‑94 (MQ=255)

                   cAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCa                                                                       <  2:3990213/94‑1 (MQ=255)

                    aaTGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAg                                                                      >  2:7435753/1‑94 (MQ=255)

                     aTGTTTTTGCATCGAGGCTGGCGAAGACCGGCTGTAGCCACTGTGCCATTTGCGGATAATCCCTCAACACCGACTCACGCACCACTGGTGCAgg                                                                     >  2:8600781/1‑94 (MQ=255)

                      tGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGgc                                                                    <  2:4919972/94‑1 (MQ=255)

                       gTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGgcg                                                                   <  1:699770/94‑1 (MQ=255)

                       gTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGgcg                                                                   >  2:7978202/1‑94 (MQ=255)

                       gTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGgcg                                                                   >  1:5606237/1‑94 (MQ=255)

                         ttttGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGcgcg                                                                 >  2:946141/1‑94 (MQ=255)

                           ttGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTa                                                               >  1:187910/1‑94 (MQ=255)

                            tGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAg                                                              >  1:8379299/1‑94 (MQ=255)

                            tGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCAAGCACCACTGGTGCAGGCGCGTAg                                                              >  1:1148022/1‑94 (MQ=255)

                              cATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGAt                                                            >  1:756970/1‑94 (MQ=255)

                               aTCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATa                                                           <  1:5786090/94‑1 (MQ=255)

                               aTCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATa                                                           <  1:1311773/94‑1 (MQ=255)

                                tCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAg                                                          <  1:4691770/94‑1 (MQ=255)

                                tCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAg                                                          <  2:2712786/94‑1 (MQ=255)

                                 ggAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCCCCGCTGGTGCAGGCGCGTAGATAgg                                                         <  2:4723797/93‑1 (MQ=255)

                                 cGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAgg                                                         >  1:6235149/1‑94 (MQ=255)

                                  gAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGt                                                        >  2:2229166/1‑94 (MQ=255)

                                   aGGCTGGCGAAGACTGTCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGtt                                                       <  2:6201445/94‑1 (MQ=255)

                                   aGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGtt                                                       >  2:1168546/1‑94 (MQ=255)

                                   aGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGtt                                                       >  1:439776/1‑94 (MQ=255)

                                     gCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGc                                                     <  1:914292/94‑1 (MQ=255)

                                     gCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGc                                                     >  2:4685612/1‑94 (MQ=255)

                                      cTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGca                                                    >  2:4146310/1‑94 (MQ=255)

                                         gCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCacac                                                 <  2:8274399/94‑1 (MQ=255)

                                          cGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACAcc                                                <  1:7505868/94‑1 (MQ=255)

                                             aGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTg                                             >  1:2180099/1‑94 (MQ=255)

                                             aGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTg                                             >  2:7511480/1‑94 (MQ=255)

                                              gACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGc                                            <  1:2304265/94‑1 (MQ=255)

                                              gACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGc                                            <  1:10182427/94‑1 (MQ=255)

                                               aCTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCg                                           <  1:6884012/94‑1 (MQ=255)

                                                cTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCgg                                          >  1:5939399/1‑94 (MQ=255)

                                                cTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCgg                                          <  2:3066008/94‑1 (MQ=255)

                                                cTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCgg                                          >  1:8464239/1‑94 (MQ=255)

                                                  ggCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGAt                                        >  1:5476681/1‑94 (MQ=255)

                                                   gCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATc                                       <  1:5584121/94‑1 (MQ=255)

                                                   gCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATc                                       <  2:1018981/94‑1 (MQ=255)

                                                   gCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATc                                       <  2:1685953/94‑1 (MQ=255)

                                                     tGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGc                                     >  1:2225641/1‑94 (MQ=255)

                                                      gTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCt                                    <  1:620004/94‑1 (MQ=255)

                                                        aGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTa                                  >  2:5350660/1‑94 (MQ=255)

                                                         gCCACTGTGCCATTTGCGGATGCTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTaa                                 <  2:8731490/94‑1 (MQ=255)

                                                         gCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTaa                                 <  1:10283894/94‑1 (MQ=255)

                                                          ccACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAg                                <  2:1002884/94‑1 (MQ=255)

                                                           cACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAgg                               >  2:1285670/1‑94 (MQ=255)

                                                           cACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAgg                               <  1:4601416/94‑1 (MQ=255)

                                                           cACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAgg                               >  2:5902855/1‑94 (MQ=255)

                                                            tcTGTGCCATTTGCGTATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGt                              <  1:4455557/93‑1 (MQ=255)

                                                            aCTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGt                              <  1:297116/94‑1 (MQ=255)

                                                            aCTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGt                              >  1:103738/1‑94 (MQ=255)

                                                             cTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGtt                             >  2:9307160/1‑94 (MQ=255)

                                                              tgtgCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGttt                            <  2:991524/94‑1 (MQ=255)

                                                              tgtgCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGttt                            >  1:10142440/1‑94 (MQ=255)

                                                              tgtgCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGttt                            <  2:3659508/94‑1 (MQ=255)

                                                                  ccATTTGCGAATACTCCCTCAACACCGACTCACGCCCCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGAGGATCGCTTAAGGTTTGCAg                        <  2:332222/94‑1 (MQ=255)

                                                                   cATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGc                       >  1:9055380/1‑94 (MQ=255)

                                                                    aTTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGcc                      <  2:5506189/94‑1 (MQ=255)

                                                                     tttGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGccc                     <  1:6469277/94‑1 (MQ=255)

                                                                        tgggATCCTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAg                  <  2:8148961/92‑1 (MQ=255)

                                                                        gCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAg                  >  1:6378644/1‑94 (MQ=255)

                                                                         cGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAgc                 <  1:9565894/94‑1 (MQ=255)

                                                                         cGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCACAgc                 >  2:8681673/1‑94 (MQ=255)

                                                                          ggATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAgcg                <  1:3172975/94‑1 (MQ=255)

                                                                          ggATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAgcg                <  1:2471431/94‑1 (MQ=255)

                                                                          ggATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAgcg                <  1:6935357/94‑1 (MQ=255)

                                                                           gATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAgcgc               >  1:8461138/1‑94 (MQ=255)

                                                                              tcTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGcgccgc            <  1:5757166/93‑1 (MQ=255)

                                                                              aCTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGcgccgc            >  1:1429687/1‑94 (MQ=255)

                                                                              aCTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGcgccgc            >  1:4170486/1‑94 (MQ=255)

                                                                              aCTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGcgccgc            >  1:3881826/1‑94 (MQ=255)

                                                                              aCTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGcgccgc            <  1:10437269/94‑1 (MQ=255)

                                                                                  ccTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTAcc        >  2:2455513/1‑94 (MQ=255)

                                                                                   cTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCg       <  1:8156368/94‑1 (MQ=255)

                                                                                   cTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCg       >  1:7548924/1‑94 (MQ=255)

                                                                                   cTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCg       <  2:8996950/94‑1 (MQ=255)

                                                                                   cTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGCTTGCAGCCCCCGCGCCCCTACCg       >  2:5889296/1‑94 (MQ=255)

                                                                                    tCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCgg      <  1:382201/94‑1 (MQ=255)

                                                                                     cAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCggg     <  1:887966/94‑1 (MQ=255)

                                                                                       acacCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGcc   <  1:5180087/94‑1 (MQ=255)

                                                                                        cacCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGCCg  <  1:8904785/94‑1 (MQ=255)

TGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACGGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGCCG  >  NC_000913/2216655‑2216836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACGGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGCCGTC  >  NC_000913/2216653‑2216838

AATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTTGAGCCACTGTGCCAATTGCCGATACTCCCTCAACACC                                                                                              >  SRR4302545.1380575/1‑94 (MQ=60)

AATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACC                                                                                              >  SRR4302545.3239969/1‑94 (MQ=60)

AATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACC                                                                                              >  SRR4302545.8998508/1‑94 (MQ=60)

AATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACC                                                                                              >  SRR4302545.3316888/1‑94 (MQ=60)

AATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACC                                                                                              >  SRR4302545.5284782/1‑94 (MQ=60)

  TGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGA                                                                                            >  SRR4302545.8592491/1‑94 (MQ=60)

    CTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACT                                                                                          <  SRR4302545.4311409/94‑1 (MQ=60)

      GGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATAATCCCTCAACACCGACTCC                                                                                        >  SRR4302545.89614/1‑94 (MQ=60)

        CATTTAGGTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACG                                                                                      <  SRR4302545.8899115/94‑1 (MQ=60)

        CATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACG                                                                                      <  SRR4302545.565446/94‑1 (MQ=60)

        CATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACG                                                                                      >  SRR4302545.6472933/1‑94 (MQ=60)

         ATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACCCACGC                                                                                     >  SRR4302545.7596942/1‑94 (MQ=60)

          TTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCACCACCGACTCACCCA                                                                                    >  SRR4302545.5735287/1‑94 (MQ=60)

          TTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCA                                                                                    >  SRR4302545.5694984/1‑94 (MQ=60)

           TCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCAC                                                                                   <  SRR4302545.6402225/94‑1 (MQ=60)

             AGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCC                                                                                 >  SRR4302545.385353/1‑94 (MQ=60)

             AGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCA                                                                                 >  SRR4302545.2000247/1‑94 (MQ=60)

              GTTGCTGCAATGTTTTTGCATTTAGGCTTGCGACGACTGGCTGTAGCAACTGTGCCGTTTGCGGATTCTCCCTCAACCCCGACTCACGCGCCAC                                                                                <  SRR4302545.5850358/94‑1 (MQ=42)

              GTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCAC                                                                                >  SRR4302545.2720732/1‑94 (MQ=60)

               TTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACT                                                                               >  SRR4302545.4411030/1‑94 (MQ=60)

               TTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACT                                                                               <  SRR4302545.3369917/94‑1 (MQ=60)

                 GCTGCAATGTTTTTGCATCGAGGCTTGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCCCGCACCACTGG                                                                             <  SRR4302545.7006201/94‑1 (MQ=60)

                  CTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGT                                                                            >  SRR4302545.3604196/1‑94 (MQ=60)

                    GCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGC                                                                          >  SRR4302545.9519194/1‑94 (MQ=60)

                    GCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGC                                                                          >  SRR4302545.7281180/1‑94 (MQ=60)

                      AATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAG                                                                        <  SRR4302545.10766361/94‑1 (MQ=60)

                       ATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGG                                                                       <  SRR4302545.10495463/94‑1 (MQ=60)

                       ATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGG                                                                       >  SRR4302545.3358778/1‑94 (MQ=60)

                       ATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGG                                                                       <  SRR4302545.10641783/94‑1 (MQ=60)

                         GTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCG                                                                     <  SRR4302545.715455/94‑1 (MQ=60)

                         GTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCG                                                                     >  SRR4302545.5755035/1‑94 (MQ=60)

                             TTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTA                                                                 >  SRR4302545.191884/1‑94 (MQ=60)

                              TGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAG                                                                >  SRR4302545.8621888/1‑94 (MQ=60)

                              TGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAG                                                                <  SRR4302545.5220996/94‑1 (MQ=60)

                              TGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCAAGCACCACTGGTGCAGGCGCGTAG                                                                >  SRR4302545.1173180/1‑94 (MQ=60)

                                CATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGAT                                                              >  SRR4302545.3681522/1‑94 (MQ=60)

                                CATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGAT                                                              >  SRR4302545.774003/1‑94 (MQ=60)

                                 ATCGAGGCTGGCGACGACTGGCCGTAGCCACTGTGCCATTTGCGGATACTCCCCCAACACCGACTCACGCACCACTCGTGCAGGCGCGTAGATA                                                             >  SRR4302545.3787927/1‑94 (MQ=60)

                                 ATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATA                                                             <  SRR4302545.1340890/94‑1 (MQ=60)

                                 ATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATA                                                             <  SRR4302545.5941663/94‑1 (MQ=60)

                                  TCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAG                                                            <  SRR4302545.4811345/94‑1 (MQ=60)

                                   CGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGG                                                           >  SRR4302545.6405081/1‑94 (MQ=60)

                                   CGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGG                                                           <  SRR4302545.5382107/94‑1 (MQ=60)

                                     AGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTT                                                         >  SRR4302545.449548/1‑94 (MQ=60)

                                     AGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTT                                                         <  SRR4302545.7754303/94‑1 (MQ=60)

                                      GGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTG                                                        <  SRR4302545.7216143/94‑1 (MQ=60)

                                      GGCTGGCGAAGACTCGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTG                                                        <  SRR4302545.4286906/94‑1 (MQ=60)

                                       GCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGC                                                       <  SRR4302545.934803/94‑1 (MQ=60)

                                         TGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCAC                                                     >  SRR4302545.386831/1‑94 (MQ=60)

                                          GGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACA                                                    <  SRR4302545.6056727/94‑1 (MQ=60)

                                          GGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACA                                                    <  SRR4302545.5698792/94‑1 (MQ=60)

                                            CGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACC                                                  <  SRR4302545.7718245/94‑1 (MQ=60)

                                            CGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACC                                                  >  SRR4302545.2334994/1‑94 (MQ=60)

                                             GAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCT                                                 >  SRR4302545.1399865/1‑94 (MQ=60)

                                               AGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTG                                               >  SRR4302545.4138112/1‑94 (MQ=60)

                                               AGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTG                                               >  SRR4302545.2233370/1‑94 (MQ=60)

                                                GACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGC                                              <  SRR4302545.2360388/94‑1 (MQ=60)

                                                GACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGC                                              <  SRR4302545.10483358/94‑1 (MQ=60)

                                                 ACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCG                                             >  SRR4302545.8668804/1‑94 (MQ=60)

                                                 ACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCG                                             <  SRR4302545.7073270/94‑1 (MQ=60)

                                                  CTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGG                                            >  SRR4302545.9389832/1‑94 (MQ=60)

                                                  CTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGG                                            >  SRR4302545.10461512/1‑94 (MQ=60)

                                                  CTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGG                                            <  SRR4302545.5927635/94‑1 (MQ=60)

                                                  CTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGG                                            >  SRR4302545.6099649/1‑94 (MQ=60)

                                                  CTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGG                                            >  SRR4302545.8708854/1‑94 (MQ=60)

                                                  CTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGG                                            >  SRR4302545.8708855/1‑94 (MQ=60)

                                                  CTCGCTGTATCCACTGTGCCATTTGCGGATTCTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTTCACACCTTGCGG                                            <  SRR4302545.7166844/94‑1 (MQ=60)

                                                    TGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCTACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGAT                                          <  SRR4302545.3911548/94‑1 (MQ=60)

                                                    TGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGAT                                          <  SRR4302545.3962978/94‑1 (MQ=60)

                                                    GGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGAT                                          <  SRR4302545.7290446/94‑1 (MQ=60)

                                                    GGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGAT                                          >  SRR4302545.5620523/1‑94 (MQ=60)

                                                     GCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATC                                         >  SRR4302545.2683210/1‑94 (MQ=60)

                                                     GCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATC                                         <  SRR4302545.5732024/94‑1 (MQ=60)

                                                     GCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATC                                         <  SRR4302545.8068890/94‑1 (MQ=60)

                                                       TGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGC                                       >  SRR4302545.2279946/1‑94 (MQ=60)

                                                        GTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCT                                      <  SRR4302545.633924/94‑1 (MQ=60)

                                                         TAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTT                                     <  SRR4302545.2502887/94‑1 (MQ=60)

                                                          AGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTA                                    >  SRR4302545.3068436/1‑94 (MQ=60)

                                                           GCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAA                                   >  SRR4302545.1245325/1‑94 (MQ=60)

                                                           GCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAA                                   <  SRR4302545.10587219/94‑1 (MQ=60)

                                                            CCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAG                                  >  SRR4302545.4658078/1‑94 (MQ=60)

                                                            CAACTGTGCCATTTGCGGATACTCCCTCAAAACCGACTCACGCACCAGTGGTGCAAGCGCGTCGCTAGGCTGCTCACCTTGCGGCTCGGTTAAG                                  >  SRR4302545.9206865/1‑94 (MQ=38)

                                                             CACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGG                                 >  SRR4302545.7152016/1‑94 (MQ=60)

                                                             CACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGG                                 <  SRR4302545.4719202/94‑1 (MQ=60)

                                                             CACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGG                                 <  SRR4302545.1879421/94‑1 (MQ=60)

                                                             CACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGG                                 >  SRR4302545.9108906/1‑94 (MQ=60)

                                                             CACTGTGACATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGAGCGTAGATAGGTTGCACAACTTGCGGAGCGGTTAAGG                                 >  SRR4302545.8309941/1‑94 (MQ=60)

                                                              TCTGTGCCATTTGCGTATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGT                                <  SRR4302545.4569137/94‑1 (MQ=60)

                                                              ACTGTGCCATTTGCGGATACTGCATCAACACCGACTCACGCTCCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGT                                <  SRR4302545.807012/94‑1 (MQ=60)

                                                              ACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGT                                <  SRR4302545.303336/94‑1 (MQ=60)

                                                              ACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGT                                >  SRR4302545.106134/1‑94 (MQ=60)

                                                              ACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGT                                <  SRR4302545.9512533/94‑1 (MQ=60)

                                                              ACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGT                                >  SRR4302545.9301193/1‑94 (MQ=60)

                                                                TGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTT                              >  SRR4302545.8874363/1‑94 (MQ=60)

                                                                TGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTT                              >  SRR4302545.10442391/1‑94 (MQ=60)

                                                                TGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTT                              <  SRR4302545.10288222/94‑1 (MQ=60)

                                                                 GTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTG                             <  SRR4302545.9138162/94‑1 (MQ=60)

                                                                   GCCATTTGCGGATACTCCATCATCACTGCCACACGCACATATGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCA                           <  SRR4302545.5193556/94‑1 (MQ=60)

                                                                     CATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGC                         >  SRR4302545.9319717/1‑94 (MQ=60)

                                                                     CATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGC                         >  SRR4302545.7783136/1‑94 (MQ=60)

                                                                      ATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCC                        <  SRR4302545.9837070/94‑1 (MQ=60)

                                                                       TTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCC                       <  SRR4302545.6646135/94‑1 (MQ=60)

                                                                          GCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAG                    >  SRR4302545.6553032/1‑94 (MQ=60)

                                                                           CGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGC                   <  SRR4302545.9847872/94‑1 (MQ=60)

                                                                           CGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGC                   <  SRR4302545.10687786/94‑1 (MQ=60)

                                                                            GGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCG                  <  SRR4302545.4548040/94‑1 (MQ=60)

                                                                            GGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCG                  <  SRR4302545.2531244/94‑1 (MQ=60)

                                                                            GGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCG                  <  SRR4302545.3250868/94‑1 (MQ=60)

                                                                            GGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCG                  <  SRR4302545.7126506/94‑1 (MQ=60)

                                                                            GGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCG                  <  SRR4302545.7126505/94‑1 (MQ=60)

                                                                             GATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGC                 <  SRR4302545.6367922/94‑1 (MQ=60)

                                                                             GATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGC                 >  SRR4302545.8705660/1‑94 (MQ=60)

                                                                                TCTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGC              <  SRR4302545.5911804/94‑1 (MQ=60)

                                                                                ACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGC              >  SRR4302545.4274561/1‑94 (MQ=60)

                                                                                ACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGC              >  SRR4302545.3978730/1‑94 (MQ=60)

                                                                                ACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGC              <  SRR4302545.5911803/94‑1 (MQ=60)

                                                                                ACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGC              <  SRR4302545.10744855/94‑1 (MQ=60)

                                                                                ACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGC              >  SRR4302545.1462122/1‑94 (MQ=60)

                                                                                 CTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCT             <  SRR4302545.7646135/94‑1 (MQ=60)

                                                                                 CTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCT             >  SRR4302545.6320070/1‑94 (MQ=60)

                                                                                    CCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACC          >  SRR4302545.6582432/1‑94 (MQ=60)

                                                                                    CCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACC          >  SRR4302545.2253363/1‑94 (MQ=60)

                                                                                     CTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCG         <  SRR4302545.4736175/94‑1 (MQ=60)

                                                                                     CTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCG         <  SRR4302545.8392140/94‑1 (MQ=60)

                                                                                     CTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCG         >  SRR4302545.7762353/1‑94 (MQ=60)

                                                                                      TCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGG        <  SRR4302545.390680/94‑1 (MQ=60)

                                                                                       CAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGG       >  SRR4302545.2920914/1‑94 (MQ=60)

                                                                                       CAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGG       <  SRR4302545.907881/94‑1 (MQ=60)

                                                                                         ACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGCC     <  SRR4302545.5313436/94‑1 (MQ=60)

                                                                                          CACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGCCG    <  SRR4302545.9163624/94‑1 (MQ=60)

                                                                                           ACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGCCGT   >  SRR4302545.3817498/1‑94 (MQ=60)

                                                                                            CCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGCCGTC  <  SRR4302545.5384611/94‑1 (MQ=60)

                                                                                            CCGACTCACACACCACTGGTGCAGGCGCGTAGATAGGTTGCACCCCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGCCGTC  <  SRR4302545.5384610/94‑1 (MQ=60)

AATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACGGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGCCGTC  >  NC_000913/2216653‑2216838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`