Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,216,746 G→C S252S (TCC→TCG)  yehZ ← putative transport system permease protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,216,7460GC100.0% 656.3 / NA 189S252S (TCC→TCG) yehZputative transport system permease protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (103/86);  total (103/86)

Only 100 of 198 total aligned reads displayed.

GCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACGGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGCC  >  NC_000913/2216656‑2216835
                                                                                          |                                                                                         
gCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGa                                                                                         >  2:4473804/1‑93 (MQ=255)
 cTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGAc                                                                                        >  1:5883490/1‑93 (MQ=255)
 cTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGAc                                                                                        <  2:1408465/93‑1 (MQ=255)
 cTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGAc                                                                                        >  1:221924/1‑93 (MQ=255)
    gCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCa                                                                                     <  2:1390049/93‑1 (MQ=255)
      aTTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCAcc                                                                                   >  1:4967628/1‑92 (MQ=255)
       ttCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGc                                                                                  >  2:1542859/1‑93 (MQ=255)
         cAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATCCTCCCTCAACACCGACTCACGcac                                                                                <  2:5313223/93‑1 (MQ=255)
          aGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGGTGTAGCCACTGTGCCGTTTGCCGGTACTACCTCAAAACCGAATCACGcatc                                                                               >  2:651654/1‑91 (MQ=255)
           gTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGcacca                                                                              >  2:3506092/1‑93 (MQ=255)
            ttGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGcaccac                                                                             >  1:4674227/1‑93 (MQ=255)
            ttGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCCGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGcaccac                                                                             >  2:5260139/1‑93 (MQ=255)
              gctgcAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTg                                                                           >  2:2152181/1‑93 (MQ=255)
               ctgcAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTgg                                                                          <  1:4649334/93‑1 (MQ=255)
                tgcAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACAACGACTCACGCACCACTGGt                                                                         >  1:3350522/1‑93 (MQ=255)
                  cAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGc                                                                       <  2:3185053/93‑1 (MQ=255)
                   aaTGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCa                                                                      <  2:5991956/93‑1 (MQ=255)
                   aaTGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGGGCa                                                                      >  2:1348306/1‑93 (MQ=255)
                    aTGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAg                                                                     <  1:4020248/93‑1 (MQ=255)
                     tGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAgg                                                                    >  2:960107/1‑93 (MQ=255)
                     tGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACCGGTGCAgg                                                                    >  1:4572933/1‑93 (MQ=255)
                        ttttGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGgcgc                                                                 <  2:1118465/93‑1 (MQ=255)
                        ttttGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGgcgc                                                                 >  2:498383/1‑93 (MQ=255)
                         tttGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGcgcg                                                                <  1:2693705/93‑1 (MQ=255)
                          ttGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGt                                                               >  1:2998832/1‑93 (MQ=255)
                           tGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTa                                                              >  1:2452166/1‑93 (MQ=255)
                           tGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTCGTGCAAGCGCGTa                                                              >  2:5754387/1‑93 (MQ=255)
                              aTCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTTGAt                                                           >  2:1457129/1‑93 (MQ=255)
                              aTCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGaa                                                           >  1:196487/1‑92 (MQ=255)
                              aTCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGAt                                                           >  1:3621495/1‑93 (MQ=255)
                              aTCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGAt                                                           <  1:414882/93‑1 (MQ=255)
                              aTCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGAt                                                           >  1:3499547/1‑93 (MQ=255)
                              aTCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGAt                                                           >  2:4914535/1‑93 (MQ=255)
                              aTCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGAt                                                           <  2:4278692/93‑1 (MQ=255)
                              aTCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACCCACCACTGGTGCAGGCGCGTAGAt                                                           >  2:2262273/1‑93 (MQ=255)
                              aTCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATAATCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGag                                                           >  2:4235601/1‑92 (MQ=255)
                               tCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATa                                                          >  1:5396281/1‑93 (MQ=255)
                                 gAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCATGCGCGTAGATAgg                                                        >  2:5875350/1‑93 (MQ=255)
                                  aGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGt                                                       <  2:5486953/93‑1 (MQ=255)
                                  aGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGt                                                       >  2:798259/1‑93 (MQ=255)
                                   ggCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGtt                                                      >  1:5716104/1‑93 (MQ=255)
                                   ggCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGCGCAGGCGCGTAGATAGGtt                                                      >  1:936110/1‑93 (MQ=255)
                                   ggCTGGCAAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGtt                                                      <  2:5960332/93‑1 (MQ=255)
                                    gCTGGGGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGAGCGTAGATAGGGTg                                                     >  1:2845035/1‑93 (MQ=255)
                                      tGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGca                                                   >  1:3883839/1‑93 (MQ=255)
                                      tGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGca                                                   >  2:2163176/1‑93 (MQ=255)
                                      tGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGca                                                   <  1:573825/93‑1 (MQ=255)
                                       ggCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGcac                                                  >  2:2848970/1‑93 (MQ=255)
                                       ggCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGcac                                                  <  2:5560917/93‑1 (MQ=255)
                                       ggCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGcac                                                  <  2:184316/93‑1 (MQ=255)
                                          gAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACAcc                                               >  1:2156071/1‑93 (MQ=255)
                                            aGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCtt                                             <  2:2583592/93‑1 (MQ=255)
                                            aGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCtt                                             <  2:2377535/93‑1 (MQ=255)
                                             gACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTg                                            <  1:6182678/93‑1 (MQ=255)
                                              aCTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGc                                           <  1:6035556/93‑1 (MQ=255)
                                               cTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCg                                          <  2:5156627/93‑1 (MQ=255)
                                               cTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCg                                          <  2:2870793/93‑1 (MQ=255)
                                                tGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCgg                                         >  1:981407/1‑93 (MQ=255)
                                                tGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCgg                                         >  2:4699494/1‑93 (MQ=255)
                                                tGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCgg                                         >  1:1910210/1‑93 (MQ=255)
                                                 ggCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGa                                        <  2:4505845/93‑1 (MQ=255)
                                                 ggCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGa                                        <  2:4790865/93‑1 (MQ=255)
                                                 ggCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGa                                        >  2:1108303/1‑93 (MQ=255)
                                                   cTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATc                                      <  1:5893596/93‑1 (MQ=255)
                                                    tGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATTGGTTGCACACCTTGCGGATCg                                     >  2:4870659/1‑93 (MQ=255)
                                                    tGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCg                                     >  2:3881934/1‑93 (MQ=255)
                                                     gTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGc                                    <  2:5933060/93‑1 (MQ=255)
                                                      tAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCt                                   >  1:717939/1‑93 (MQ=255)
                                                        gCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCGCTTGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTa                                 <  1:4547908/93‑1 (MQ=255)
                                                         ccACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTaa                                <  1:3757003/93‑1 (MQ=255)
                                                         ccACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTaa                                <  2:3909371/93‑1 (MQ=255)
                                                          cACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAg                               >  1:1072436/1‑93 (MQ=255)
                                                          cACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAg                               <  1:1481438/93‑1 (MQ=255)
                                                           aCTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAgg                              >  1:1561088/1‑93 (MQ=255)
                                                            cTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGt                             <  1:4259048/93‑1 (MQ=255)
                                                            cTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGt                             >  1:610955/1‑93 (MQ=255)
                                                             tgtgCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGtt                            >  2:104534/1‑93 (MQ=255)
                                                             tgtgCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGtt                            >  2:4738116/1‑93 (MQ=255)
                                                              gtgCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGttt                           >  1:3116712/1‑93 (MQ=255)
                                                                 ccATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCa                        <  2:1880516/93‑1 (MQ=255)
                                                                   aTTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGc                      >  1:6411955/1‑93 (MQ=255)
                                                                    tttGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGcc                     >  2:1492046/1‑93 (MQ=255)
                                                                    tttGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGcc                     >  2:3813949/1‑93 (MQ=255)
                                                                    tttGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGcc                     >  2:3649954/1‑93 (MQ=255)
                                                                    tttGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGcc                     <  2:2083384/93‑1 (MQ=255)
                                                                    tttGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGcc                     <  1:4336987/93‑1 (MQ=255)
                                                                     ttGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGccc                    <  2:2772570/93‑1 (MQ=255)
                                                                     ttGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGccc                    >  1:1431288/1‑93 (MQ=255)
                                                                      tGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACCCCTTGCGGATCGCTTAAGGTTTGCAGcccc                   <  1:1337581/93‑1 (MQ=255)
                                                                      tGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGcccc                   <  2:3136873/93‑1 (MQ=255)
                                                                       gCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCa                  <  1:4083048/93‑1 (MQ=255)
                                                                         ggATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAgc                <  1:3228243/93‑1 (MQ=255)
                                                                          gATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAgcg               <  1:2549039/93‑1 (MQ=255)
                                                                            tACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGcgcc             <  2:4121595/93‑1 (MQ=255)
                                                                                cccTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTa         >  1:5260089/1‑93 (MQ=255)
                                                                                 ccTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTAc        >  2:3401195/1‑93 (MQ=255)
                                                                                 ccTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTAc        <  2:2428569/93‑1 (MQ=255)
                                                                                      acacCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGc   >  1:1062065/1‑93 (MQ=255)
                                                                                      acacCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGCTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGc   >  1:4760915/1‑93 (MQ=255)
                                                                                       cacCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTCCAGCCCCAGCGCCGCTACCGGGcc  <  2:6231602/93‑1 (MQ=255)
                                                                                          |                                                                                         
GCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACGGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGCC  >  NC_000913/2216656‑2216835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACGGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGCCGTCA  >  NC_000913/2216653‑2216839
                                                                                             |                                                                                             
AATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACC                                                                                               <  SRR4302537.3239196/94‑1 (MQ=60)
AATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACC                                                                                               <  SRR4302537.3746527/94‑1 (MQ=60)
 ATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGACGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCG                                                                                              >  SRR4302537.3749022/1‑94 (MQ=60)
   GCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGAC                                                                                            >  SRR4302537.223507/1‑94 (MQ=60)
   GCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGAC                                                                                            >  SRR4302537.5923861/1‑94 (MQ=60)
    CTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACT                                                                                           >  SRR4302537.736294/1‑94 (MQ=60)
     TGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTC                                                                                          >  SRR4302537.6482007/1‑94 (MQ=60)
        CATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACC                                                                                       >  SRR4302537.5001865/1‑94 (MQ=60)
         ATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACCC                                                                                      >  SRR4302537.144081/1‑94 (MQ=60)
         ATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCACTTGCGGATACTCCCTCAACACCGACTCACGC                                                                                      >  SRR4302537.5903311/1‑94 (MQ=60)
          TTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCA                                                                                     <  SRR4302537.1516457/94‑1 (MQ=60)
              GTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGGTGTAGCCACTGTGCCATTTGCGGATAGTCCCTCAACATCGACTCACGCACCAC                                                                                 >  SRR4302537.449518/1‑94 (MQ=60)
              GTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCAC                                                                                 >  SRR4302537.4706563/1‑94 (MQ=60)
                  CTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGT                                                                             <  SRR4302537.4681476/94‑1 (MQ=60)
                  CTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGT                                                                             >  SRR4302537.4205067/1‑94 (MQ=60)
                  CTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACCCACCACTGGG                                                                             >  SRR4302537.984824/1‑94 (MQ=60)
                  CTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACAACGACTCACGCACCACTGGT                                                                             >  SRR4302537.3374245/1‑94 (MQ=60)
                   TGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTG                                                                            >  SRR4302537.2280313/1‑94 (MQ=60)
                    GCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGA                                                                           >  SRR4302537.3945081/1‑94 (MQ=60)
                     CAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCA                                                                          >  SRR4302537.3907658/1‑94 (MQ=60)
                     CAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCA                                                                          >  SRR4302537.5769676/1‑94 (MQ=60)
                       ATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGG                                                                        <  SRR4302537.4048394/94‑1 (MQ=60)
                       ATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGG                                                                        <  SRR4302537.5700165/94‑1 (MQ=60)
                       ATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACCGGTGCAGG                                                                        >  SRR4302537.4604586/1‑94 (MQ=60)
                         GTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCG                                                                      <  SRR4302537.5938850/94‑1 (MQ=60)
                            TTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGT                                                                   <  SRR4302537.4705990/94‑1 (MQ=60)
                            TTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGT                                                                   <  SRR4302537.2712529/94‑1 (MQ=60)
                            TTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGT                                                                   >  SRR4302537.3019738/1‑94 (MQ=60)
                             TTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTA                                                                  >  SRR4302537.2469419/1‑94 (MQ=60)
                                CATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGAT                                                               >  SRR4302537.3524308/1‑94 (MQ=60)
                                CATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGAT                                                               >  SRR4302537.3647016/1‑94 (MQ=60)
                                CATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGAT                                                               >  SRR4302537.2509342/1‑94 (MQ=60)
                                CATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGAA                                                               >  SRR4302537.197885/1‑94 (MQ=60)
                                 ATCGAGGCTGGCGAAGACTGGGTGTAGTCACTGTGCCATTTGCGGATACTCCCTTTAAACCGACTCACGCACCACTAGTGCGGGCGCCTAGATA                                                              >  SRR4302537.4227219/1‑94 (MQ=53)
                                 ATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATA                                                              >  SRR4302537.5433126/1‑94 (MQ=60)
                                 ATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATA                                                              <  SRR4302537.417755/94‑1 (MQ=60)
                                 ATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATA                                                              <  SRR4302537.4306144/94‑1 (MQ=60)
                                    GAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGT                                                           >  SRR4302537.4976954/1‑94 (MQ=60)
                                     AGGCTGGCGAGGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTT                                                          <  SRR4302537.472653/94‑1 (MQ=60)
                                     AGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTT                                                          <  SRR4302537.304681/94‑1 (MQ=60)
                                     AGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTT                                                          >  SRR4302537.5755222/1‑94 (MQ=60)
                                     AGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGCGCAGGCGCGTAGATAGGTT                                                          >  SRR4302537.942561/1‑94 (MQ=60)
                                      GGCTGGGGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGAGCGTAGATAGGGTG                                                         >  SRR4302537.2864951/1‑94 (MQ=60)
                                        CTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCA                                                       >  SRR4302537.268288/1‑94 (MQ=60)
                                        CTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCA                                                       >  SRR4302537.3911007/1‑94 (MQ=60)
                                         TGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCAC                                                      <  SRR4302537.577765/94‑1 (MQ=60)
                                          GGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACA                                                     <  SRR4302537.4591464/94‑1 (MQ=60)
                                            CGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACC                                                   >  SRR4302537.2171267/1‑94 (MQ=60)
                                            CGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACC                                                   >  SRR4302537.2067468/1‑94 (MQ=60)
                                              AAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTT                                                 >  SRR4302537.2890026/1‑94 (MQ=60)
                                                GACTGGCTGTAGCCACTGTGTCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGC                                               <  SRR4302537.3729030/94‑1 (MQ=60)
                                                GACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGC                                               <  SRR4302537.6225264/94‑1 (MQ=60)
                                                 ACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCG                                              <  SRR4302537.2234323/94‑1 (MQ=60)
                                                 ACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCG                                              <  SRR4302537.6077029/94‑1 (MQ=60)
                                                  CTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGG                                             >  SRR4302537.988183/1‑94 (MQ=60)
                                                  CTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGG                                             >  SRR4302537.1923471/1‑94 (MQ=60)
                                                      CTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCG                                         <  SRR4302537.5934035/94‑1 (MQ=60)
                                                        GTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCT                                       >  SRR4302537.722844/1‑94 (MQ=60)
                                                           GCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCGCTTGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAA                                    <  SRR4302537.4579383/94‑1 (MQ=60)
                                                            CCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAG                                   >  SRR4302537.1079828/1‑94 (MQ=60)
                                                            CCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAG                                   <  SRR4302537.3783377/94‑1 (MQ=60)
                                                             TACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGG                                  >  SRR4302537.1571981/1‑94 (MQ=60)
                                                             CACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGG                                  >  SRR4302537.3329810/1‑94 (MQ=60)
                                                             CACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGG                                  >  SRR4302537.4160581/1‑94 (MQ=60)
                                                             CACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGG                                  <  SRR4302537.1491742/94‑1 (MQ=60)
                                                             CACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGG                                  >  SRR4302537.409202/1‑94 (MQ=60)
                                                              CCTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGT                                 <  SRR4302537.1332946/94‑1 (MQ=60)
                                                              ACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGT                                 >  SRR4302537.615128/1‑94 (MQ=60)
                                                               CTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTT                                <  SRR4302537.4288646/94‑1 (MQ=60)
                                                               CTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTT                                <  SRR4302537.1865352/94‑1 (MQ=60)
                                                                TGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTT                               <  SRR4302537.1955446/94‑1 (MQ=60)
                                                                TGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTT                               >  SRR4302537.6419949/1‑94 (MQ=60)
                                                                TGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTT                               <  SRR4302537.5324141/94‑1 (MQ=60)
                                                                TGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTT                               >  SRR4302537.3138478/1‑94 (MQ=60)
                                                                 GTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTG                              >  SRR4302537.3565075/1‑94 (MQ=60)
                                                                 GTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTG                              <  SRR4302537.4538376/94‑1 (MQ=60)
                                                                     CATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGC                          >  SRR4302537.6455575/1‑94 (MQ=60)
                                                                      ATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCC                         >  SRR4302537.3449919/1‑94 (MQ=60)
                                                                       TTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCC                        <  SRR4302537.4367094/94‑1 (MQ=60)
                                                                       TTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCC                        >  SRR4302537.1441253/1‑94 (MQ=60)
                                                                         TGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACCCCTTGCGGATCGCTTAAGGTTTGCAGCCCCA                      <  SRR4302537.1346898/94‑1 (MQ=60)
                                                                         TGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCA                      <  SRR4302537.4641755/94‑1 (MQ=60)
                                                                          GCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAG                     <  SRR4302537.4111551/94‑1 (MQ=60)
                                                                            GGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCG                   <  SRR4302537.3250992/94‑1 (MQ=60)
                                                                             GATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGC                  <  SRR4302537.2566896/94‑1 (MQ=60)
                                                                              ATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCC                 >  SRR4302537.5281257/1‑94 (MQ=60)
                                                                                 CTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCT              >  SRR4302537.5929531/1‑94 (MQ=60)
                                                                                  TCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTA             >  SRR4302537.5296283/1‑94 (MQ=60)
                                                                                       CAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGG        >  SRR4302537.1464452/1‑94 (MQ=60)
                                                                                       CAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGG        >  SRR4302537.2737488/1‑94 (MQ=60)
                                                                                        AACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGC       >  SRR4302537.1069387/1‑94 (MQ=60)
                                                                                        AACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGCTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGC       >  SRR4302537.4793799/1‑94 (MQ=60)
                                                                                         ACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGCC      >  SRR4302537.6176152/1‑94 (MQ=60)
                                                                                         ACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGCC      <  SRR4302537.6111451/94‑1 (MQ=60)
                                                                                         ACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGCC      <  SRR4302537.3202912/94‑1 (MQ=60)
                                                                                           ACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGCCGT    >  SRR4302537.3024598/1‑94 (MQ=60)
                                                                                            CCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGCCGTC   <  SRR4302537.3651648/94‑1 (MQ=60)
                                                                                             CGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGCCGTCA  >  SRR4302537.2705215/1‑94 (MQ=60)
                                                                                             CGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGCCGTCA  >  SRR4302537.6497773/1‑94 (MQ=60)
                                                                                             CGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGCCGTCA  >  SRR4302537.4067112/1‑94 (MQ=60)
                                                                                             CGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGCCGTCA  >  SRR4302537.4314198/1‑94 (MQ=60)
                                                                                             |                                                                                             
AATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACGGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGCCGTCA  >  NC_000913/2216653‑2216839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑