Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,235,796 T→C H505R (CAC→CGC)  mglA ← MglA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,235,7960TC98.6% 921.9 / ‑0.7 275H505R (CAC→CGC) mglAMglA
Reads supporting (aligned to +/- strand):  ref base T (2/2);  new base C (148/123);  total (150/125)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.85e-01

Only 100 of 283 total aligned reads displayed.

TAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATAC  >  NC_000913/2235705‑2235886
                                                                                           |                                                                                          
tAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGgcgc                                                                                          <  2:9333526/94‑1 (MQ=255)
tAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGgcgc                                                                                          <  2:1905143/94‑1 (MQ=255)
 aaaaGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCa                                                                                         >  2:405457/1‑94 (MQ=255)
  aaaGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCaa                                                                                        >  2:3382659/1‑94 (MQ=255)
    aGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAaag                                                                                      <  1:2624216/94‑1 (MQ=255)
      aaCGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAaagac                                                                                    >  1:38930/1‑94 (MQ=255)
       acgacgTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAaagacg                                                                                   <  1:6726292/94‑1 (MQ=255)
       acgacgTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAaagacg                                                                                   <  2:3816589/94‑1 (MQ=255)
       acgacgTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAaagacg                                                                                   <  2:637342/94‑1 (MQ=255)
        cgacgTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAaagacgc                                                                                  <  2:10222604/94‑1 (MQ=255)
         gacgTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAaagacgca                                                                                 >  1:1814989/1‑94 (MQ=255)
          gcgTAAGGACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAaagacgcaa                                                                                <  2:1195698/93‑1 (MQ=255)
          acgTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAaagacgcaa                                                                                >  2:10112044/1‑94 (MQ=255)
           cgTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAaagacgcaag                                                                               <  1:1451986/94‑1 (MQ=255)
              aaaTACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAaagacgcaagacg                                                                            >  2:573183/1‑94 (MQ=255)
                  aCCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGa                                                                        <  1:9104280/94‑1 (MQ=255)
                  aCCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGa                                                                        >  1:1005432/1‑94 (MQ=255)
                    cGCCCTCTTTCAGGTAAGTAAGAAAGCTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAAt                                                                      <  1:6334559/94‑1 (MQ=255)
                      cccTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAAttt                                                                    <  2:8597828/94‑1 (MQ=255)
                       ccTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTc                                                                   >  1:3419585/1‑94 (MQ=255)
                       ccTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTc                                                                   >  2:6460492/1‑94 (MQ=255)
                        ctctTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCg                                                                  >  1:5017705/1‑94 (MQ=255)
                           tttCAGGTAAGTAAGAACACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGttt                                                               <  2:9381190/94‑1 (MQ=255)
                           tttCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGttt                                                               >  2:4339301/1‑94 (MQ=255)
                            ttCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGtttt                                                              >  1:2096387/1‑94 (MQ=255)
                             tCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTg                                                             <  2:6081599/94‑1 (MQ=255)
                             tCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTg                                                             <  2:7068440/94‑1 (MQ=255)
                              cAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGc                                                            >  2:6616072/1‑94 (MQ=255)
                              cAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGc                                                            >  1:10331053/1‑94 (MQ=255)
                              cAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGAAGCAAGACGCAGAATTTCGTTTTgg                                                            >  2:1477740/1‑93 (MQ=255)
                               aGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCg                                                           >  2:9979853/1‑94 (MQ=255)
                                 gtaagtaaGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCgtt                                                         >  1:10565186/1‑94 (MQ=255)
                                 gtaagtaaGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCgtt                                                         >  1:4205105/1‑94 (MQ=255)
                                   aagtaagAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCgttgt                                                       >  2:10458797/1‑94 (MQ=255)
                                   aagtaagAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCgttgt                                                       <  1:5507803/94‑1 (MQ=255)
                                   aagtaagAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCgttgt                                                       >  2:6684349/1‑94 (MQ=255)
                                    agtaagAAACCTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCgttgtt                                                      <  1:323594/94‑1 (MQ=255)
                                    agtaagAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCgttgtt                                                      >  2:1318732/1‑94 (MQ=255)
                                    agtaagAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCgttgta                                                      <  1:7051008/94‑2 (MQ=255)
                                     gtaagAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCgttgttg                                                     >  2:7417426/1‑94 (MQ=255)
                                     gtaagAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCgttgttg                                                     <  1:792350/94‑1 (MQ=255)
                                      taagAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCgttgttgt                                                    >  2:5523567/1‑94 (MQ=255)
                                      taagAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCgttgttgt                                                    <  2:9644461/94‑1 (MQ=255)
                                       aagAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCgttgttgtt                                                   >  1:3736959/1‑94 (MQ=255)
                                       aagAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCgttgttgtt                                                   >  1:6482934/1‑94 (MQ=255)
                                       aagAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCgttgttgtt                                                   >  2:2086527/1‑94 (MQ=255)
                                       aagAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCgttgttggt                                                   >  1:1437287/1‑92 (MQ=255)
                                        agAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGttt                                                  >  1:9637666/1‑94 (MQ=255)
                                        agAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGttt                                                  >  2:252936/1‑94 (MQ=255)
                                         gAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGtttt                                                 >  2:3200679/1‑94 (MQ=255)
                                         gAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGtttt                                                 >  1:7382232/1‑94 (MQ=255)
                                         aaaaaCCTTTTTTATTTAACGCCCTCATGGGAAGCCCCTAAGCCTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGtttt                                                 <  2:3486180/93‑1 (MQ=255)
                                          aaaaCTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGttttt                                                >  1:8861943/1‑94 (MQ=255)
                                              cTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTAt                                            >  2:7838594/1‑94 (MQ=255)
                                               ttttCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATc                                           <  1:7712552/94‑1 (MQ=255)
                                               ttttCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATc                                           >  1:1748319/1‑94 (MQ=255)
                                               ttttCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATc                                           <  1:4697776/94‑1 (MQ=255)
                                                tttCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCg                                          <  1:6215699/94‑1 (MQ=255)
                                                tttCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCg                                          <  2:2470614/94‑1 (MQ=255)
                                                tttCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCg                                          <  2:6068390/94‑1 (MQ=255)
                                                tttCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCg                                          <  2:2841392/94‑1 (MQ=255)
                                                   cTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACa                                       <  2:8353866/94‑1 (MQ=255)
                                                   cTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACa                                       >  2:3158999/1‑94 (MQ=255)
                                                    ttATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACaa                                      >  1:5598858/1‑94 (MQ=255)
                                                     tATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAAt                                     <  1:6054648/94‑1 (MQ=255)
                                                      aTTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAAtt                                    >  2:689531/1‑94 (MQ=255)
                                                       tttAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTc                                   >  2:9445654/1‑94 (MQ=255)
                                                        ttAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTcc                                  <  1:6626335/94‑1 (MQ=255)
                                                         tAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCg                                 <  2:2580229/94‑1 (MQ=255)
                                                           aCGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGa                               <  1:8514052/94‑1 (MQ=255)
                                                            cGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGaa                              >  2:6166519/1‑94 (MQ=255)
                                                             gCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTTCGTTGTTGTTTTTGTATCGACAATTCCGGaaa                             <  1:670238/94‑1 (MQ=255)
                                                              cACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAAc                            >  2:5203615/1‑94 (MQ=255)
                                                              cACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAAc                            >  1:4934516/1‑94 (MQ=255)
                                                               aCTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACg                           >  1:9291565/1‑94 (MQ=255)
                                                                cTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACga                          <  1:4115301/94‑1 (MQ=255)
                                                                 tCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACgag                         >  2:8951348/1‑94 (MQ=255)
                                                                 tCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACgag                         >  2:76291/1‑94 (MQ=255)
                                                                  cATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACgaga                        <  1:3334207/94‑1 (MQ=255)
                                                                  cATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACgaga                        >  2:8009809/1‑94 (MQ=255)
                                                                     gggAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCa                     <  2:2730923/94‑1 (MQ=255)
                                                                       gAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCAtt                   <  2:4761244/94‑1 (MQ=255)
                                                                          gCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGTAACGAGACCATTGCt                <  1:9546554/94‑1 (MQ=255)
                                                                            cccTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCa              >  1:9734113/1‑94 (MQ=255)
                                                                             ccTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCAt             <  2:4619555/94‑1 (MQ=255)
                                                                               tAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGa           >  1:1614370/1‑94 (MQ=255)
                                                                                aaTCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGAc          <  2:1672268/94‑1 (MQ=255)
                                                                                aaTCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGAc          >  1:10390538/1‑94 (MQ=255)
                                                                                aaTCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGAc          >  1:2902264/1‑94 (MQ=255)
                                                                                 aTCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGAcc         >  2:4207378/1‑94 (MQ=255)
                                                                                 aTCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGAca         >  1:867738/1‑93 (MQ=255)
                                                                                     tAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGaa     <  1:8341160/94‑1 (MQ=255)
                                                                                     tAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGaa     >  2:5433597/1‑94 (MQ=255)
                                                                                     tAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGaa     >  2:9580812/1‑94 (MQ=255)
                                                                                     tAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGaa     <  1:7496668/94‑1 (MQ=255)
                                                                                      aaaGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAAt    <  2:10130915/94‑1 (MQ=255)
                                                                                      aaaGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAAt    >  2:670598/1‑94 (MQ=255)
                                                                                      aaaGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAAt    >  2:10019988/1‑94 (MQ=255)
                                                                                       aaGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATa   >  2:6782195/1‑94 (MQ=255)
                                                                                        aGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATAc  <  1:1172590/94‑1 (MQ=255)
                                                                                           |                                                                                          
TAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATAC  >  NC_000913/2235705‑2235886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGG  >  NC_000913/2235703‑2235888
                                                                                             |                                                                                            
ACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGC                                                                                              >  SRR4302545.8981976/1‑94 (MQ=60)
    AAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAA                                                                                          >  SRR4302545.7696134/1‑94 (MQ=60)
     AAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAA                                                                                         >  SRR4302545.4553022/1‑94 (MQ=60)
      AGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAG                                                                                        <  SRR4302545.2687391/94‑1 (MQ=60)
        AACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGAC                                                                                      <  SRR4302545.8844825/94‑1 (MQ=60)
        AACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGAC                                                                                      <  SRR4302545.8844826/94‑1 (MQ=60)
        AACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGAC                                                                                      <  SRR4302545.1270451/94‑1 (MQ=60)
        AACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGAC                                                                                      >  SRR4302545.39828/1‑94 (MQ=60)
         ACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACG                                                                                     <  SRR4302545.6910702/94‑1 (MQ=60)
         ACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACG                                                                                     >  SRR4302545.1474406/1‑94 (MQ=60)
           GACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCA                                                                                   >  SRR4302545.10166676/1‑94 (MQ=60)
           GACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCA                                                                                   >  SRR4302545.1858964/1‑94 (MQ=60)
             CGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAG                                                                                 <  SRR4302545.1485158/94‑1 (MQ=60)
              GTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGA                                                                                >  SRR4302545.5127222/1‑94 (MQ=60)
               TAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGAC                                                                               <  SRR4302545.9847407/94‑1 (MQ=60)
                AAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAACGACGCAAGACG                                                                              <  SRR4302545.5411287/94‑1 (MQ=60)
                AAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACG                                                                              <  SRR4302545.8571302/94‑1 (MQ=60)
                AAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACG                                                                              >  SRR4302545.1524646/1‑94 (MQ=60)
                AAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACG                                                                              >  SRR4302545.2148306/1‑94 (MQ=60)
                AAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACG                                                                              <  SRR4302545.696498/94‑1 (MQ=60)
                  ATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCA                                                                            <  SRR4302545.7766126/94‑1 (MQ=60)
                  ATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCA                                                                            >  SRR4302545.7154022/1‑94 (MQ=60)
                   TACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAG                                                                           <  SRR4302545.119088/94‑1 (MQ=60)
                    ACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGA                                                                          <  SRR4302545.9371238/94‑1 (MQ=60)
                    ACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGA                                                                          >  SRR4302545.1027671/1‑94 (MQ=60)
                      CGCCCTCTTTCAGGTAAGTAAGAAAGCTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAAT                                                                        <  SRR4302545.6507355/94‑1 (MQ=60)
                      CGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAAT                                                                        <  SRR4302545.6507354/94‑1 (MQ=60)
                        CCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTT                                                                      <  SRR4302545.2722541/94‑1 (MQ=60)
                         CCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTC                                                                     >  SRR4302545.1740466/1‑94 (MQ=60)
                         CCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTC                                                                     >  SRR4302545.3503110/1‑94 (MQ=60)
                          TTCTTTCAGGTAAGTAAGAAATCGTTGCTTAGCTAACGCTCTCATGGAAAGCCCCTAATCTTAAAGGCGCTAAGTCGCAAGAAGCAGAATTTCG                                                                    <  SRR4302545.6906648/94‑1 (MQ=30)
                          CTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCG                                                                    >  SRR4302545.5144902/1‑94 (MQ=60)
                          CTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCG                                                                    <  SRR4302545.6906649/94‑1 (MQ=60)
                          CTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAAGTTCG                                                                    >  SRR4302545.5928993/1‑94 (MQ=60)
                            CTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTT                                                                  >  SRR4302545.5657128/1‑94 (MQ=60)
                            CTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTT                                                                  <  SRR4302545.9862109/94‑1 (MQ=60)
                            CTTACAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTT                                                                  <  SRR4302545.4225636/94‑1 (MQ=60)
                             TTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTT                                                                 <  SRR4302545.1737443/94‑1 (MQ=60)
                             TGTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTT                                                                 <  SRR4302545.6392638/94‑1 (MQ=60)
                              TTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTT                                                                >  SRR4302545.2147926/1‑94 (MQ=60)
                              TTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTT                                                                <  SRR4302545.9721937/94‑1 (MQ=60)
                                CAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGC                                                              >  SRR4302545.10635645/1‑94 (MQ=60)
                                 AGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCG                                                             >  SRR4302545.1804415/1‑94 (MQ=60)
                                  GGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGT                                                            <  SRR4302545.406024/94‑1 (MQ=60)
                                  GGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGT                                                            >  SRR4302545.3883929/1‑94 (MQ=60)
                                   GTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAACGACGCAAGACGCAGAATTTCGTTTTGCGTT                                                           >  SRR4302545.10876596/1‑94 (MQ=60)
                                   GTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTT                                                           >  SRR4302545.4310102/1‑94 (MQ=60)
                                   GTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTT                                                           >  SRR4302545.762125/1‑94 (MQ=60)
                                   GTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTT                                                           >  SRR4302545.10876420/1‑94 (MQ=60)
                                   GTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTT                                                           >  SRR4302545.3832296/1‑94 (MQ=60)
                                     AAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGT                                                         <  SRR4302545.5653035/94‑1 (MQ=60)
                                      AGTAAGAAACCTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTT                                                        <  SRR4302545.330471/94‑1 (MQ=60)
                                      AGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTA                                                        <  SRR4302545.7245896/94‑1 (MQ=60)
                                       GTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTG                                                       <  SRR4302545.810168/94‑1 (MQ=60)
                                       GTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTG                                                       >  SRR4302545.161960/1‑94 (MQ=60)
                                       GTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTG                                                       <  SRR4302545.2682819/94‑1 (MQ=60)
                                        TAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGT                                                      >  SRR4302545.9563792/1‑94 (MQ=60)
                                         AAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTT                                                     <  SRR4302545.10423923/94‑1 (MQ=60)
                                         AAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTT                                                     >  SRR4302545.6660201/1‑94 (MQ=60)
                                         AAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTT                                                     >  SRR4302545.3828894/1‑94 (MQ=60)
                                         AAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGGT                                                     >  SRR4302545.1469960/1‑94 (MQ=60)
                                          AGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTT                                                    >  SRR4302545.9921800/1‑94 (MQ=60)
                                           GAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTT                                                   <  SRR4302545.3477639/94‑1 (MQ=60)
                                           GAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTT                                                   >  SRR4302545.5553995/1‑94 (MQ=60)
                                           GAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTT                                                   >  SRR4302545.7590470/1‑94 (MQ=60)
                                            AAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTT                                                  >  SRR4302545.9119376/1‑94 (MQ=60)
                                            AAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTT                                                  >  SRR4302545.1934475/1‑94 (MQ=60)
                                             AAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTG                                                 >  SRR4302545.3187042/1‑94 (MQ=60)
                                               ACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTA                                               >  SRR4302545.6740638/1‑94 (MQ=60)
                                                CTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTAT                                              >  SRR4302545.9097509/1‑94 (MQ=60)
                                                ATTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCGGAATTTCGTTTTGCGTTGTTGTTTTTGTAT                                              <  SRR4302545.7540200/94‑1 (MQ=60)
                                                 TTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATC                                             >  SRR4302545.1790155/1‑94 (MQ=60)
                                                 TTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATC                                             <  SRR4302545.4817488/94‑1 (MQ=60)
                                                 TTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATC                                             <  SRR4302545.7930891/94‑1 (MQ=60)
                                                 TTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATC                                             >  SRR4302545.3675005/1‑94 (MQ=60)
                                                  TTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCG                                            <  SRR4302545.6384916/94‑1 (MQ=60)
                                                   TTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGA                                           >  SRR4302545.208158/1‑94 (MQ=60)
                                                     CTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACA                                         <  SRR4302545.10017809/94‑1 (MQ=60)
                                                     CTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACA                                         <  SRR4302545.9368462/94‑1 (MQ=60)
                                                      TTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAA                                        >  SRR4302545.5747377/1‑94 (MQ=60)
                                                       TATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAAT                                       <  SRR4302545.6218784/94‑1 (MQ=60)
                                                         TTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTC                                     >  SRR4302545.861679/1‑94 (MQ=60)
                                                         TTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTC                                     >  SRR4302545.6632580/1‑94 (MQ=60)
                                                         TTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTC                                     <  SRR4302545.3330805/94‑1 (MQ=60)
                                                          TTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCC                                    <  SRR4302545.1819327/94‑1 (MQ=60)
                                                          TTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCC                                    <  SRR4302545.6807954/94‑1 (MQ=60)
                                                           TAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCG                                   >  SRR4302545.7532750/1‑94 (MQ=60)
                                                           TAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCG                                   >  SRR4302545.10661001/1‑94 (MQ=60)
                                                            AACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGG                                  <  SRR4302545.9974340/94‑1 (MQ=60)
                                                             ACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGA                                 <  SRR4302545.8760037/94‑1 (MQ=60)
                                                             ACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGA                                 >  SRR4302545.6535539/1‑94 (MQ=60)
                                                             ACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGA                                 <  SRR4302545.6292656/94‑1 (MQ=60)
                                                               GCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTTCGTTGTTGTTTTTGTATCGACAATTCCGGAAA                               <  SRR4302545.685166/94‑1 (MQ=60)
                                                               GCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAA                               >  SRR4302545.4918290/1‑94 (MQ=60)
                                                                CACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAAC                              >  SRR4302545.5059636/1‑94 (MQ=60)
                                                                 ACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACG                             <  SRR4302545.891514/94‑1 (MQ=60)
                                                                 ACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACG                             >  SRR4302545.9563972/1‑94 (MQ=60)
                                                                  CTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGA                            >  SRR4302545.6810977/1‑94 (MQ=60)
                                                                  CTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGA                            <  SRR4302545.4218271/94‑1 (MQ=60)
                                                                  CTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGA                            >  SRR4302545.3405783/1‑94 (MQ=60)
                                                                    CATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGA                          <  SRR4302545.3415687/94‑1 (MQ=60)
                                                                      TGGGAGGCCCCTCATCTTACAGGCGCAAAGACGCAAGACGCAGTATTTCGTTTTGCGTTGTTGTTTTTGTATCGACCATTCCGGAAACGAAACC                        <  SRR4302545.9695334/94‑1 (MQ=60)
                                                                       GGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCA                       <  SRR4302545.4322012/94‑1 (MQ=60)
                                                                       GGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCA                       >  SRR4302545.10658339/1‑94 (MQ=60)
                                                                       GGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCA                       <  SRR4302545.865850/94‑1 (MQ=60)
                                                                        GGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCAT                      <  SRR4302545.8093008/94‑1 (MQ=60)
                                                                          AAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTG                    >  SRR4302545.1066336/1‑94 (MQ=60)
                                                                            GCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGTAACGAGACCATTGCT                  <  SRR4302545.9827795/94‑1 (MQ=60)
                                                                              CCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCA                >  SRR4302545.10023917/1‑94 (MQ=60)
                                                                              CCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCA                <  SRR4302545.4831397/94‑1 (MQ=60)
                                                                               CCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCAT               <  SRR4302545.5090261/94‑1 (MQ=60)
                                                                               CCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCAT               >  SRR4302545.6269538/1‑94 (MQ=60)
                                                                                CTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATG              >  SRR4302545.8394401/1‑94 (MQ=60)
                                                                                 TAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGA             <  SRR4302545.3428454/94‑1 (MQ=60)
                                                                                 TAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGA             >  SRR4302545.1652714/1‑94 (MQ=60)
                                                                                 TAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCCCATGA             <  SRR4302545.427586/94‑1 (MQ=60)
                                                                                  AATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTTGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGAC            >  SRR4302545.10697034/1‑94 (MQ=60)
                                                                                  AATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGAC            >  SRR4302545.10696866/1‑94 (MQ=60)
                                                                                  AATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGAC            >  SRR4302545.2972627/1‑94 (MQ=60)
                                                                                   ATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACC           <  SRR4302545.9130885/94‑1 (MQ=60)
                                                                                   ATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACA           >  SRR4302545.887200/1‑94 (MQ=60)
                                                                                     CTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAG         <  SRR4302545.10211761/94‑1 (MQ=60)
                                                                                     CTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAG         >  SRR4302545.6060347/1‑94 (MQ=60)
                                                                                       TAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAA       <  SRR4302545.7708821/94‑1 (MQ=60)
                                                                                       TAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAA       <  SRR4302545.8582835/94‑1 (MQ=60)
                                                                                        AAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAAT      >  SRR4302545.138119/1‑94 (MQ=60)
                                                                                        AAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAAT      >  SRR4302545.7920372/1‑94 (MQ=60)
                                                                                         AAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATA     >  SRR4302545.6772737/1‑94 (MQ=60)
                                                                                         AAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATA     <  SRR4302545.3630670/94‑1 (MQ=60)
                                                                                          AGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATAC    <  SRR4302545.1198572/94‑1 (MQ=60)
                                                                                          AGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATAC    >  SRR4302545.3312104/1‑94 (MQ=60)
                                                                                           GGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACG   >  SRR4302545.8216356/1‑94 (MQ=60)
                                                                                           GGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACG   <  SRR4302545.8468242/94‑1 (MQ=60)
                                                                                            GCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGG  <  SRR4302545.7029966/94‑1 (MQ=60)
                                                                                             |                                                                                            
ACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGG  >  NC_000913/2235703‑2235888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑