Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,235,796 T→C H505R (CAC→CGC)  mglA ← MglA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,235,7960TC100.0% 674.7 / NA 194H505R (CAC→CGC) mglAMglA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (109/85);  total (109/85)

Only 100 of 199 total aligned reads displayed.

AAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATA  >  NC_000913/2235706‑2235885
                                                                                          |                                                                                         
aaaaGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGgcgc                                                                                         >  1:5480972/1‑93 (MQ=255)
aaaaGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGgcgc                                                                                         >  2:2164780/1‑93 (MQ=255)
 aaaGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCa                                                                                        >  1:766873/1‑93 (MQ=255)
 aaaGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCa                                                                                        >  2:1052441/1‑93 (MQ=255)
  aaGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCaa                                                                                       <  2:4900726/93‑1 (MQ=255)
    gAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAaag                                                                                     >  1:2298362/1‑93 (MQ=255)
     aaCGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAaaga                                                                                    >  1:6395010/1‑93 (MQ=255)
       cgacgTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAaagacg                                                                                  <  2:5745130/93‑1 (MQ=255)
        gacgTTAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAaagacgc                                                                                 >  2:3711458/1‑93 (MQ=255)
         acgTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAaagacgca                                                                                >  2:4793683/1‑93 (MQ=255)
          cgTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAaagacgcaa                                                                               >  2:5710124/1‑93 (MQ=255)
          cgTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAaagacgcaa                                                                               <  1:827356/93‑1 (MQ=255)
            tAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAaagacgcaaga                                                                             <  1:2953584/93‑1 (MQ=255)
            tAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAaagacgcaaga                                                                             >  1:3080624/1‑93 (MQ=255)
            tAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAaagacgcaaga                                                                             >  1:6120182/1‑93 (MQ=255)
            tAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAaagacgcaaga                                                                             <  1:1524492/93‑1 (MQ=255)
             aaaTACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAaagacgcaagac                                                                            <  2:404872/93‑1 (MQ=255)
              aaTACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAaagacgcaagacg                                                                           <  1:1645491/93‑1 (MQ=255)
                 cccGCCCTCTTTCAGGTAATTAAGAAAACTTTTCTTATTTAACGCACTCATGTGAAGCCCCTAATCTTAAAGGCGCAAAGACGAAAGACGCAg                                                                        <  2:157796/92‑1 (MQ=255)
                 aCCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAg                                                                        >  2:4329967/1‑93 (MQ=255)
                  ccGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGa                                                                       <  1:6075410/93‑1 (MQ=255)
                   cGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGaa                                                                      <  2:334789/93‑1 (MQ=255)
                   cGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGaa                                                                      <  2:2620994/93‑1 (MQ=255)
                    gCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAAt                                                                     >  1:1151317/1‑93 (MQ=255)
                     cccTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCTCTCATGGGAAGCCCTTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAAtt                                                                    <  2:5760581/93‑1 (MQ=255)
                     cccTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGCCGCAGAAtt                                                                    >  1:1730683/1‑93 (MQ=255)
                     cccTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAAtt                                                                    >  1:883545/1‑93 (MQ=255)
                     cccTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAAtt                                                                    <  2:910881/93‑1 (MQ=255)
                     cccTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAAtt                                                                    <  2:4603821/93‑1 (MQ=255)
                     cccTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAAtt                                                                    >  1:2668499/1‑93 (MQ=255)
                      ccTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAAttt                                                                   <  2:164513/93‑1 (MQ=255)
                      ccTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAAttt                                                                   >  1:1288591/1‑93 (MQ=255)
                       ctctTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTc                                                                  >  1:2897960/1‑93 (MQ=255)
                         ctTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGt                                                                >  1:3493538/1‑93 (MQ=255)
                          tttCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGtt                                                               <  2:5003881/93‑1 (MQ=255)
                           ttCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGttt                                                              <  2:5961671/93‑1 (MQ=255)
                            tCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGtttt                                                             <  2:5653016/93‑1 (MQ=255)
                              aGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTga                                                           >  1:5027053/1‑92 (MQ=255)
                               ggTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCg                                                          >  1:226827/1‑93 (MQ=255)
                                gtaagtaaGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGCCGCAAGACGCAGAATTTCGTTTTGCgt                                                         >  2:4279615/1‑93 (MQ=255)
                                   agtaagAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCgttgt                                                      >  1:2072536/1‑93 (MQ=255)
                                   agtaagAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCgttgt                                                      <  1:3889769/93‑1 (MQ=255)
                                    gtaagAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCgttgtt                                                     <  1:5301272/93‑1 (MQ=255)
                                      aagAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCgttgttgt                                                   >  1:2455409/1‑93 (MQ=255)
                                      aagAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCgttgttgt                                                   <  2:3830275/93‑1 (MQ=255)
                                         aaaaCTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGtttt                                                >  1:3518934/1‑93 (MQ=255)
                                          aaaCTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGttttt                                               <  1:4648931/93‑1 (MQ=255)
                                          aaaCTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGttttt                                               >  2:6235122/1‑93 (MQ=255)
                                          aaaCTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGttttt                                               <  1:5279525/93‑1 (MQ=255)
                                            aCTTTTCTTATTTGACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGt                                             >  1:3390809/1‑93 (MQ=255)
                                            aCTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGt                                             >  2:5598660/1‑93 (MQ=255)
                                             cTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTa                                            <  2:1077184/93‑1 (MQ=255)
                                             cTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTa                                            <  2:3603241/93‑1 (MQ=255)
                                             cTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTa                                            >  2:2330639/1‑93 (MQ=255)
                                             cTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTa                                            >  1:2714434/1‑93 (MQ=255)
                                             cTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTa                                            <  1:4507885/93‑1 (MQ=255)
                                              ttttCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTAt                                           >  2:3072017/1‑93 (MQ=255)
                                                ttCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCg                                         >  1:6312048/1‑93 (MQ=255)
                                                    tATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACaa                                     >  1:2364223/1‑93 (MQ=255)
                                                     aTTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAAt                                    <  1:3296455/93‑1 (MQ=255)
                                                       ttAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAAttg                                  <  1:933949/93‑2 (MQ=255)
                                                       ttAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTc                                  <  1:1380634/93‑1 (MQ=255)
                                                        tAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTcc                                 <  1:2282633/93‑1 (MQ=255)
                                                         aaCGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCg                                <  2:2087524/93‑1 (MQ=255)
                                                           cGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGa                              >  1:557406/1‑93 (MQ=255)
                                                            gCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGaa                             >  1:1051925/1‑93 (MQ=255)
                                                             cACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGaaa                            <  1:5185164/93‑1 (MQ=255)
                                                              aCTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAAc                           >  1:5662212/1‑93 (MQ=255)
                                                               cTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACg                          >  2:6357186/1‑93 (MQ=255)
                                                               cTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACg                          >  1:467931/1‑93 (MQ=255)
                                                                tCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACga                         <  2:5370796/93‑1 (MQ=255)
                                                                tCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACga                         >  2:316807/1‑93 (MQ=255)
                                                                tCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACga                         >  2:6459198/1‑93 (MQ=255)
                                                                tCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACga                         >  2:691317/1‑93 (MQ=255)
                                                                   tGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGAc                      >  2:2439255/1‑93 (MQ=255)
                                                                    gggAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGAcc                     <  1:4717576/93‑1 (MQ=255)
                                                                     ggAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGAcct                    >  2:2200752/1‑92 (MQ=255)
                                                                     ggAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCa                    <  2:4255654/93‑1 (MQ=255)
                                                                     ggAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCa                    <  1:4430686/93‑1 (MQ=255)
                                                                     ggAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCa                    >  1:4109397/1‑93 (MQ=255)
                                                                     ggAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCa                    <  2:1772158/93‑1 (MQ=255)
                                                                      gAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCAt                   <  2:5207196/93‑1 (MQ=255)
                                                                      gAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCAt                   <  2:1356431/93‑1 (MQ=255)
                                                                         gCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGc                >  1:493663/1‑93 (MQ=255)
                                                                            ccTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCTTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCa             >  1:5736256/1‑93 (MQ=255)
                                                                            ccTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCa             <  1:4888195/93‑1 (MQ=255)
                                                                              tAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATg           >  2:3807929/1‑93 (MQ=255)
                                                                              tAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATg           >  2:1230492/1‑93 (MQ=255)
                                                                               aaTCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGa          >  2:1520558/1‑93 (MQ=255)
                                                                                aTCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGAc         <  2:4674233/93‑1 (MQ=255)
                                                                                aTCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGAc         <  2:3243449/93‑1 (MQ=255)
                                                                                  cTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCa       <  2:5508091/93‑1 (MQ=255)
                                                                                  cTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCa       <  1:6158623/93‑1 (MQ=255)
                                                                                   ttAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAg      >  2:2062880/1‑93 (MQ=255)
                                                                                    tAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGa     >  1:5332153/1‑93 (MQ=255)
                                                                                     aaaGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGaa    >  1:700925/1‑93 (MQ=255)
                                                                                     aaaGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGaa    <  1:3134607/93‑1 (MQ=255)
                                                                                     aaaGGCGAAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGaa    <  2:6012840/93‑1 (MQ=255)
                                                                                      aaGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAAt   <  2:4736311/93‑1 (MQ=255)
                                                                                       aGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATa  <  2:5894650/93‑1 (MQ=255)
                                                                                          |                                                                                         
AAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATA  >  NC_000913/2235706‑2235885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGT  >  NC_000913/2235703‑2235889
                                                                                             |                                                                                             
ACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGC                                                                                               >  SRR4302537.3059974/1‑94 (MQ=60)
ACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGC                                                                                               >  SRR4302537.2836802/1‑94 (MQ=60)
ACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGC                                                                                               <  SRR4302537.2400319/94‑1 (MQ=60)
ACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGC                                                                                               >  SRR4302537.2327516/1‑94 (MQ=60)
ACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGC                                                                                               <  SRR4302537.5398371/94‑1 (MQ=60)
ACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGC                                                                                               >  SRR4302537.4518785/1‑94 (MQ=60)
 CTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCG                                                                                              <  SRR4302537.3265007/94‑1 (MQ=60)
 CTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCG                                                                                              <  SRR4302537.2757797/94‑1 (MQ=60)
 CTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCG                                                                                              >  SRR4302537.4739250/1‑94 (MQ=60)
 CTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCG                                                                                              >  SRR4302537.172120/1‑94 (MQ=60)
  TAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGC                                                                                             >  SRR4302537.5518391/1‑94 (MQ=60)
   AAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCA                                                                                            >  SRR4302537.772122/1‑94 (MQ=60)
    AAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAA                                                                                           >  SRR4302537.2317344/1‑94 (MQ=60)
     AAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAA                                                                                          >  SRR4302537.3054241/1‑94 (MQ=60)
     AAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAA                                                                                          >  SRR4302537.6196152/1‑94 (MQ=60)
      AGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAG                                                                                         >  SRR4302537.2314585/1‑94 (MQ=60)
       GAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGA                                                                                        >  SRR4302537.6438562/1‑94 (MQ=60)
        AACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGAC                                                                                       <  SRR4302537.246607/94‑1 (MQ=60)
           GACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCA                                                                                    <  SRR4302537.5201811/94‑1 (MQ=60)
             CGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAG                                                                                  <  SRR4302537.833021/94‑1 (MQ=60)
              GTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGA                                                                                 >  SRR4302537.3102078/1‑94 (MQ=60)
              GTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGA                                                                                 >  SRR4302537.6162249/1‑94 (MQ=60)
               TAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGAC                                                                                <  SRR4302537.1535140/94‑1 (MQ=60)
               TAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGAC                                                                                <  SRR4302537.1426291/94‑1 (MQ=60)
               TAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGAC                                                                                <  SRR4302537.1742064/94‑1 (MQ=60)
               TAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGAC                                                                                <  SRR4302537.2974312/94‑1 (MQ=60)
                AAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACG                                                                               >  SRR4302537.3127696/1‑94 (MQ=60)
                AAATACCGACCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACG                                                                               <  SRR4302537.6473511/94‑1 (MQ=60)
                 AATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGC                                                                              <  SRR4302537.1657019/94‑1 (MQ=60)
                 AATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGC                                                                              >  SRR4302537.340612/1‑94 (MQ=60)
                  ATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCA                                                                             >  SRR4302537.265616/1‑94 (MQ=60)
                   TACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAG                                                                            >  SRR4302537.468284/1‑94 (MQ=60)
                     CCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAA                                                                          <  SRR4302537.6117162/94‑1 (MQ=60)
                     CCGCCCTCTTTCAGCTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAA                                                                          >  SRR4302537.5702419/1‑94 (MQ=60)
                      CGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAAT                                                                         >  SRR4302537.1159322/1‑94 (MQ=60)
                       GCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGCCGCAGAATT                                                                        >  SRR4302537.1742763/1‑94 (MQ=60)
                       GCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATT                                                                        >  SRR4302537.2687142/1‑94 (MQ=60)
                       GCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATT                                                                        >  SRR4302537.889625/1‑94 (MQ=60)
                        CCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTT                                                                       >  SRR4302537.1297555/1‑94 (MQ=60)
                        CCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTT                                                                       >  SRR4302537.2824021/1‑94 (MQ=60)
                        CCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTT                                                                       >  SRR4302537.1325994/1‑94 (MQ=60)
                         CCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTC                                                                      >  SRR4302537.2918322/1‑94 (MQ=60)
                         CCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTC                                                                      >  SRR4302537.5888217/1‑94 (MQ=60)
                         CCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTC                                                                      <  SRR4302537.5535818/94‑1 (MQ=60)
                          CTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCG                                                                     >  SRR4302537.4939215/1‑94 (MQ=60)
                           TCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGT                                                                    >  SRR4302537.3518250/1‑94 (MQ=60)
                            CTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTT                                                                   >  SRR4302537.3540879/1‑94 (MQ=60)
                                CAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGA                                                               >  SRR4302537.5061668/1‑94 (MQ=60)
                                 AGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCG                                                              >  SRR4302537.534090/1‑94 (MQ=60)
                                 AGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCG                                                              >  SRR4302537.228435/1‑94 (MQ=60)
                                   GTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTT                                                            >  SRR4302537.4729303/1‑94 (MQ=60)
                                   GTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTT                                                            >  SRR4302537.3949748/1‑94 (MQ=60)
                                     AAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGT                                                          >  SRR4302537.2087135/1‑94 (MQ=60)
                                     AAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGT                                                          >  SRR4302537.735576/1‑94 (MQ=60)
                                     AAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGT                                                          >  SRR4302537.6135294/1‑94 (MQ=60)
                                     AAGTAAGAAAACTATTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGT                                                          >  SRR4302537.3511367/1‑94 (MQ=60)
                                      AGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTT                                                         <  SRR4302537.784920/94‑1 (MQ=60)
                                      AGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTT                                                         <  SRR4302537.3916975/94‑1 (MQ=60)
                                       GTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTG                                                        <  SRR4302537.5337683/94‑1 (MQ=60)
                                        TAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGT                                                       >  SRR4302537.2472678/1‑94 (MQ=60)
                                          AGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTT                                                     >  SRR4302537.1997581/1‑94 (MQ=60)
                                           GAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTT                                                    >  SRR4302537.3543833/1‑94 (MQ=60)
                                             AAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTG                                                  >  SRR4302537.2290747/1‑94 (MQ=60)
                                             AAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTG                                                  <  SRR4302537.4681070/94‑1 (MQ=60)
                                             AAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTG                                                  <  SRR4302537.5315845/94‑1 (MQ=60)
                                             AAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTG                                                  >  SRR4302537.1242511/1‑94 (MQ=60)
                                              AACTTTTCTTATTTGACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGT                                                 >  SRR4302537.3414829/1‑94 (MQ=60)
                                               ACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTA                                                >  SRR4302537.2733389/1‑94 (MQ=60)
                                               ACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTA                                                >  SRR4302537.6428473/1‑94 (MQ=60)
                                               ACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTA                                                <  SRR4302537.912044/94‑1 (MQ=60)
                                                CTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTAT                                               <  SRR4302537.4539058/94‑1 (MQ=60)
                                                CTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTAT                                               <  SRR4302537.1645269/94‑1 (MQ=60)
                                                 TTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATC                                              >  SRR4302537.4975136/1‑94 (MQ=60)
                                                 TTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATC                                              >  SRR4302537.6323431/1‑94 (MQ=60)
                                                  TTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCG                                             >  SRR4302537.6355257/1‑94 (MQ=60)
                                                  TTTCTTATTTAACGCACTCATGGGAAACCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCG                                             >  SRR4302537.3728706/1‑94 (MQ=60)
                                                   TTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGA                                            >  SRR4302537.4281328/1‑94 (MQ=60)
                                                    TCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGAC                                           >  SRR4302537.2687666/1‑94 (MQ=60)
                                                     CTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACA                                          <  SRR4302537.857424/94‑1 (MQ=60)
                                                      TTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAA                                         >  SRR4302537.2380894/1‑94 (MQ=60)
                                                       TATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAAT                                        >  SRR4302537.2224244/1‑94 (MQ=60)
                                                        ATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATT                                       <  SRR4302537.3319756/94‑1 (MQ=60)
                                                          TTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTGC                                     <  SRR4302537.940383/94‑1 (MQ=60)
                                                          TTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCC                                     <  SRR4302537.1390268/94‑1 (MQ=60)
                                                           TAACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCG                                    <  SRR4302537.2298738/94‑1 (MQ=60)
                                                             ACGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGA                                  >  SRR4302537.561245/1‑94 (MQ=60)
                                                              CGCACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAA                                 >  SRR4302537.1059182/1‑94 (MQ=60)
                                                                CACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAAC                               <  SRR4302537.5220831/94‑1 (MQ=60)
                                                                CACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAAC                               >  SRR4302537.5700956/1‑94 (MQ=60)
                                                                 ACTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACG                              >  SRR4302537.471143/1‑94 (MQ=60)
                                                                  CTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCGGGAAACGA                             >  SRR4302537.4630668/1‑94 (MQ=60)
                                                                  CTCATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGA                             >  SRR4302537.4761571/1‑94 (MQ=60)
                                                                    CATGGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCCAGACGCAGAATTTCGTTTTGCGTTGTTGCTTTTGTATCGACAATTCCGGAAACGAGA                           >  SRR4302537.3302543/1‑94 (MQ=60)
                                                                       GGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCA                        <  SRR4302537.4750200/94‑1 (MQ=60)
                                                                       GGGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCA                        >  SRR4302537.4138108/1‑94 (MQ=60)
                                                                        GGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCAT                       <  SRR4302537.2970636/94‑1 (MQ=60)
                                                                        GGAAGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCAT                       <  SRR4302537.4461284/94‑1 (MQ=60)
                                                                           AGCCCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGC                    >  SRR4302537.497065/1‑94 (MQ=60)
                                                                              CCCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCTTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCA                 >  SRR4302537.5775532/1‑94 (MQ=60)
                                                                               CCTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCAT                <  SRR4302537.4921949/94‑1 (MQ=60)
                                                                                CTAATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATG               <  SRR4302537.4531901/94‑1 (MQ=60)
                                                                                CTAATATTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTACCGACAATTCCGGAAACGAGACCATTGCTCATG               <  SRR4302537.2312978/94‑1 (MQ=60)
                                                                                   ATCTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATAACC            >  SRR4302537.5223031/1‑94 (MQ=60)
                                                                                     CTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAG          >  SRR4302537.5642358/1‑94 (MQ=60)
                                                                                     CTTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAG          <  SRR4302537.6201005/94‑1 (MQ=60)
                                                                                      TTAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGA         >  SRR4302537.5368727/1‑94 (MQ=60)
                                                                                       TAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAA        >  SRR4302537.705720/1‑94 (MQ=60)
                                                                                       TAAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAA        <  SRR4302537.536966/94‑1 (MQ=60)
                                                                                        AAAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAAT       <  SRR4302537.3156604/94‑1 (MQ=60)
                                                                                         AAGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGANACCATTGCTCATGACCAGANTA      >  SRR4302537.5328332/1‑94 (MQ=60)
                                                                                          AGGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATAC     >  SRR4302537.3967603/1‑94 (MQ=60)
                                                                                           GGCGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACG    >  SRR4302537.4862731/1‑94 (MQ=60)
                                                                                             CGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGT  <  SRR4302537.4729280/94‑1 (MQ=60)
                                                                                             CGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGT  >  SRR4302537.3010261/1‑94 (MQ=60)
                                                                                             CGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGT  >  SRR4302537.2294625/1‑94 (MQ=60)
                                                                                             CGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGT  <  SRR4302537.4897368/94‑1 (MQ=60)
                                                                                             CGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGT  >  SRR4302537.4508679/1‑94 (MQ=60)
                                                                                             |                                                                                             
ACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGT  >  NC_000913/2235703‑2235889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑